51 st International Congress of Meat Science and Technology
|
|
|
- Leonard Cooper
- 5 years ago
- Views:
Transcription
1 Use of Comparative Genomics as a Tool to Assess the Clinical and Public Health Significance of Emerging Shiga toxin Producing Escherichia coli Serotypes 51 st International Congress of Meat Science and Technology Baltimore, MD, August 9 th, 2005 Mohamed A. Karmali, MB ChB, FRCP(C) Public Health Agency of Canada
2 Bloody Stools from a Patient with STEC O157:H7 associated Hemorrhagic Colitis
3 Clinical Features of Infection by Shiga toxin producing E. Coli (STEC) O157:H7 Abdominal pain Low grade or absent fever Diarrhea Hemorrhagic colitis Hemolytic uremic syndrome (HUS)
4 Advanced Hemorrhagic/necrotic Colitis in a Fatal Case of STEC associated HUS
5 Renal Histopathology in a Fatal Case of STECassociated HUS. Evidence of Glomerulus Microangiopathy
6
7 Public Health Impact of the Hemolytic Uremic Syndrome (HUS) Leading cause of acute renal failure in children Mortality rate 0.5 3% (higher in the elderly) Up to 40% of survivors develop chronic renal disease or hypertension Occasionally long term involvement of other organ systems, e.g., pancreas, GI tract, CNS
8 Epidemiology of Escherichia coli O157:H7 Outbreaks, United States, JM Rangel et al., Emerg. Infect. Dis. 11:603 9, to 2002 (350 outbreaks 49 States) Cases 8,598 Hospitalizations 1,493 (17%) HUS 354 (4%) Deaths 40 (0.5%)
9 Epidemiology of Escherichia coli O157:H7 Outbreaks, United States, JM Rangel et al., Emerg. Infect. Dis. 11:603 9, 2005 Transmission Routes (350 Outbreaks) 14% 21% 9% 3% 53% Foodborne Unknown Person to person Waterborne Animal contact
10 Epidemiology of Escherichia coli O157:H7 Outbreaks, United States, JM Rangel et al., Emerg. Infect. Dis. 11:603 9, 2005 Roast beef (5) Steak (2) Sirloin tip (1) Salami (1) 21% 6% 5% 4% Food vehicle (183 outbreaks) 23% 41% Ground Beef Unknown Produce Other beef Other food vehicle Dairy product
11
12 STEC Virulence Factors Shiga toxins Stx1, Stx2, Stx2 variants Attaching and effacing lesion (LEE PAI) TSSS, Intimin, Tir, effector molecules p O157 plasmid mediated factors hlycabd, katp, espp, etpc etpo, Product with homology to clostridial toxins Miscellaneous factors efa1, iha, chua, and many others
13 EVOLUTION OF BACTERIAL VARIANTS BY ACQUISITION AND LOSS OF GENETIC INFORMATION U. Dobrindt et al., Nature Reviews (Microbiology) 2: , 2004 Common bacterial ancestor Genome reduction by deletion events Gene acquisition by HGT Mutations, Rearrangements GEI Plasmid
14 HORIZONTAL GENE TRANSFER Plasmids Bacteriophages Transposons Integrons Genomic Islands Ecological islands Symbiosis islands Saprophytic islands Pathogenicity islands
15 Pathogenicity Islands (PAIs) Large blocks of apparently foreign genes, found uniquely in (more) pathogenic members of a genus or species Hacker J and Kaper J (2001) The Concept of Pathogenicity Islands. In JB Kaper and J Hacker (Eds.). Pathogenicity islands and other mobile virulence elements (pp 1 11). ASM Press, Washington DC.
16 Examples of PAIs UPEC kb, hemolysins, P fimbriae, and CNF EPEC/EHEC LEE (35 43 kb) Salmonella SPIs 1 5 (17 40 kb) Shigella flexneri SHI I and SHI II (~ 50 kb) Yersinia spp. HPI (~ 40 kb) Vibrio cholerae VPI (40 kb Tcp adhesin) H. pylori cag (40 kb) S. aureus SaPI 1 (15 kb; TSST 1)
17 The Locus of Enterocyte Effacement JB Kaper, Centre for Vaccine Development, U of Maryland School of Medicine
18 TYPE III SECRETION NEEDLE COMPLEX A. Blocker et al. Mol. Microbiol 39: , 2001 A. Blocker et al., Mol. Microbiol. 39: , 2001
19 Characteristic Attaching &Effacing Colonization of Epithelial Cells by LEE +ve EPEC S. Knutton, U of Birmingham
20 Classification of VTEC Serotypes Into Seropathotypes Seropathotype A Relative Incidence Outbreaks Severe Disease Serotypes LEE B Moderate Uncommon Yes O26:H11; O103:H2 O111:NM O111:H8 O145:NM C D E O91:H21 Low Rare Yes O104:H21 /+ O113:H21 others Low Rare No Multiple /+ High Common Yes O157:H7 O157:H Nonhuman N/A N/A Multiple /+ + +
21 Genome Sequence of Enterohaemorrhagic Escherichia coli O157:H7 Perna T, Et Al: Nature 2001, 409: EDL 933 genome (5.5 Mb) has 1 million more base pairs and 1387 more genes than the commensal E. coli K 12 strain MG1655 The extra genes are organized mostly in gene clusters known as O islands
22 Comparative Genomics Approaches to Investigate Non O157 VTEC Targeted survey of EDL 933 O islands Subtractive hybridization DNA Microarrays Genome walking Multi locus sequence typing (MLST)
23 O Island #122 in the genome of EDL 933 (O157:H7) 23,029 bp (26 ORFs) 0 5 kb 10 kb Z4316 Z4318 Z4323 phev trna Int pagc Z4322 Z4325 sen Z4313 Z4314 Z4315 Z4317 Z4321 Z4324 Z4326 ISA (ISEc8) Z4320 ISA (p. transposase) Z kb Z kb Z4331 efa1 efa1 Z4336 Z4338 Z4328 Z4329 Z4330 Z4332 Z4333 Z4334 Z4337 Z4340 ISA (p. transposase) IS629 ISA ISEc8
24 Study Strains (N=70) Karmali et al, J. Clin. Microbiol. 41: , 2003 SEROPATHO TYPE NUMBER STRAINS A 13 O157:H7 (10 strains) O157:NM (3 strains) B 15 SEROTYPES O26:H11 (3); O103:H2 (3); O111:NM (3); O121:H19 (3); O145:NM (3) C 14 D 14 E 14 O5:NM (2); O91:H21 (4); O104:H21 (1); O113:H21 (4); O121:NM (2); O165:H25 (1) O7:H4 (1), O69:H11 (1); O103:H25 (2); O113:H4 (1), O117:H7 (2);O119:H25 (1); O132:NM (1); O146:H21 (1), O171:H2 (1), O172:NM (1), O174:H8 (1), OR:H2 (1) O6:H34 (1); O8:H19 (1); O39:H49 (1): O46:H38 (1); O76:H7 (1); O84:NM (1); O88:H25 (1); O98:H25 (1); O113:NM (1) O136:NM (1); O136:H12 (1); O153:H31 (1) O156:NM (1); O163:NM (1)
25 Distribution of OI#122 genes in representative strains in different seropathotypes (A to E) and controls. Karmali et al, J. Clin. Microbiol. 41: , 2003 Control (A) Control (A) Control ( ) (B) EDL933 Sakai K12 CL1 L L L L (C) (D) (E) (D) CL3 EC2 267 EC3 377 N02 L L L L Kb 600bp Lanes: L, 100bp ladder; 1, pagc (Z4321); 2, sen (Z4326); 3, efa1 (Z4332); 4, efa1 (Z4333)
26 Frequency of Complete, Incomplete, and Absent OI 122 in 70 VTEC Strains Karmali et al, J. Clin. Microbiol. 41: , % Positive "Complete" "Incomplete" "Absent"
27 Seropathotype Distribution of Complete, Incomplete, and Absent OI 122 in 70 (N=70) Karmali et al, J. Clin. Microbiol. 41: , 2003 % +ve A B C D E Complete Incomplete Absent Seropathotype
28 Differences in Distribution of COI#122 in VTEC Seropathotypes Karmali et al, J. Clin. Microbiol. 41: , 2003 Seropathotype (% Strains +ve for COI#122) A, B (78.6) Associated with epidemic disease A, B, C (62.0) Associated with serious disease (HUS) Seropathotype (% Strains +ve for COI#122) C, D, E (14.3) Not associated with epidemic disease D, E (7.1) Not associated with serious disease (HUS) SIGNIFICANCE P< OR =22 95% CI P< OR = % CI
29 Relationship Between the Presence of Intact OI#122 and LEE Karmali et al, J. Clin. Microbiol. 41: , 2003 % +ve A B C D E Intact OI#122 LEE Seropathotype
30 Seropathotype Distribution of Stx1, Stx2, & HlyA Karmali et al, J. Clin. Microbiol. 41: , 2003 % +VE A B C D E Stx1 Stx2 hlya SEROPATHOTYPE
31 Frequency of Complete, Incomplete, and Absent OI 122 in 70 VTEC Strains Karmali et al, J. Clin. Microbiol. 41: , % Positive "Complete" "Incomplete" "Absent"
32 Analysis of Incomplete OI pagc +VE sen, efa1, efa1 VE No. Strains sen, efa1, efa1+ve pagc VE A B C D E Seropathotype 3 Genes 2 Genes 1 Gene
33 Genetic Organization of EPEC, EHEC, and RDEC 1 LEE C. Zhu et al. Infect. Immun. 69: , 2001 M. Tauschek et al. Mol. Microbiol. 44: , 2002 LEE EPEC (E2348/69) IS600 selc LEE EHEC (EDL 933) Prophage 933L selc int LEE RDEC 1 (63/39) efa1/ lifa sena pheu trna The LEE Core Region
34 Analysis of Incomplete OI pagc +VE sen, efa1, efa1 VE No. Strains sen, efa1, efa1+ve pagc VE A B C D E Seropathotype 3 Genes 2 Genes 1 Gene
35 Genetic and Physical Map of the Sequenced Region of the Genomic Island of E. coli O113:H21 Strain CL3 Shen et al. Infect. Immun. 72: , kb BsiWI EcoRI BsiWI EcoRI EcoRI BsiWI Probe E2 B1 Probe E1 Z1635 Z1637 S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 S12 S13 S14 Z Z1643 Z1644 Yp Yp Y. Pestis ad/ha t t X H Z4321 Z1636 Z Z H8dr 1 H8dr 2 Z DR12 I I DR12 OI#122 OI#48 GS I GS II OI#
36 Why do strains of Seropathotype A cause HUS and Epidemic Disease More Often than Seropathotype B Strains? SUBTRACTIVE HYBRIDIZATION between EDL 933 (O157:H7) Seropathotype A and CLI (O26:H11) Seropathotype B
37 Four Fimbria Encoding Genomic Islands that are Highly Specific for STEC O157 Strains Shen et al. J. Clin. Microbiol. 43: , 2005 OI 1 5M 0 OI 154 ORI OI 141 E. Coli O157:H7 EDL 933 1M OI 47 3M
38 Distribution of OI #s 1, 47, 141,& 154 in EDL 933 (O157:H7) and CL1 (O26:H11) bp A B M bp M OI 1 OI 141 OI Ctrl OI Ctrl EDL933 CL1 EDL933 CL1 Figure 1. Ethidium bromide stained agarose gel showing the results of PCR investigation of the existence of OIs 1, 47, 141 and 154 in E. coli strain CL1 (serotype O26:H11). EDL933 were used as positive control. ORFs are designaed as following: Panel A, OI 1: 1, Z ; 2, Z0022; 3, Z0023; 4, Z0024; 5, Z0025. OI 141: 6, Z4965; 7, Z4966; 8, Z4968m; 9, Z4969; 10, Z4971. OI 154: 11, Z5220; 12, Z5221; 13, Z5222; 14, Z , 15, Z5225. Panel B, ORFs in OI 47: 1, Z1528; 2, Z1530; 3, Z1531; 4, Z1533; 5, Z1534; 6, Z1535; 7, Z1536; 8, Z1537; 9, Z1538; 10, Z1539; 11, Z ; 12, Z1542; 13, Z1543; 14, Z1544; 15, Z1545; 16, Z1546; 17, Z1547; 18, Z1548; 19, Z1549; 20, Z1550; 21, Z1551; 22, Z1552; 23, Z1553; 24, Z1554; 25, Z1555; 26, Z1556. M, DNA size marker; Ctrl, 23S rrna gene.
39 Seropathotype distribution of OIs # 1, 47, 141, and SPT A SPT B SPT C SPT D SPT E OI 1 OI 47 OI 141 OI 154
40 Public Health Applications of Comparative Genomics Diagnosis & Epidemiology Molecular Risk Assessment Detection / surveillance of emerging pathogens Detection of engineered BT pathogens Treatment Elimination from reservoirs Microbiology as a predictive discipline
41 Acknowledgements LFZ M Karmali M Mascarenhas S Shen K Ziebell S Johnson R. Reid Smith K. Rahn S. Ralston Robyn Morgan Shirley Yung NML C Clark CPHLN J Isaac Renton U Maryland J Kaper CIHR LFZ M Karmali, V Gannon U of Guelph C. Whitfield, C. Gyles U of British Columbia B. Finlay, U of Toronto P Sherman VIDO A Potter
Applicability of Phylogenetic Methods for Characterizing the Public Health Significance of Verocytotoxin-Producing Escherichia coli Strains
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2008, p. 1671 1675 Vol. 74, No. 5 0099-2240/08/$08.00 0 doi:10.1128/aem.01619-07 Copyright 2008, American Society for Microbiology. All Rights Reserved. Applicability
Received 21 May 2003/Returned for modification 22 July 2003/Accepted 20 August 2003
JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 2003, p. 4930 4940 Vol. 41, No. 11 0095-1137/03/$08.00 0 DOI: 10.1128/JCM.41.11.4930 4940.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved.
Shiga Toxin producing E. coli (STEC) in food which serotypes are important? Lothar Beutin
BUNDESINSTITUT FÜR RISIKOBEWERTUNG Shiga Toxin producing E. coli (STEC) in food which serotypes are important? Lothar Beutin National Reference Laboratory for Escherichia coli Federal Institute for Risk
Shiga toxin-producing Escherichia coli
Shiga toxin-producing Escherichia coli Terry Arthur Research Microbiologist Meat Safety and Quality Research Unit U.S. Meat Animal Research Center Use of product names by USDA implies no approval to the
Discrimination of enterohemorrhagic E. coli (EHEC) from non-ehec strains. based on detection of various combinations of non-lee-encoded type III
JCM Accepts, published online ahead of print on 24 July 2013 J. Clin. Microbiol. doi:10.1128/jcm.01471-13 Copyright 2013, American Society for Microbiology. All Rights Reserved. 1 2 3 Discrimination of
Foodborne Outbreak of E. coli Infections and Hemolytic Uremic Syndrome in Germany, 2011
Foodborne Outbreak of E. coli Infections and Hemolytic Uremic Syndrome in Germany, 2011 Kirk Smith, DVM, MS, PhD Supervisor Foodborne, Vectorborne and Zoonotic Diseases Unit Minnesota Department of Health
Update on infections with and clinical lab guidelines for Shiga toxin-producing E. coli (STEC) in the United States
Update on infections with and clinical lab guidelines for Shiga toxin-producing E. coli (STEC) in the United States Patricia M. Griffin, MD Enteric Diseases Epidemiology Branch Centers for Disease Control
Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Apr. 2008, p. 2153 2160 Vol. 74, No. 7 0099-2240/08/$08.00 0 doi:10.1128/aem.02566-07 Copyright 2008, American Society for Microbiology. All Rights Reserved. Molecular
Are all VTEC created Equal?
PHL-HSE-Dublin Mid Leinster Are all VTEC created Equal? Anne Carroll Escherichia coli Commensal Microrganism but some strains are cause of infections in humans Syndromes associated to E. coli infections:
GI Bacterial Infections (part-1)
GI Bacterial Infections (part-1) Mohammed Abdulla Mehdi FIBMS (internal medicine), FIBMS (Gastroenterology & Hepatology) Acute diarrhea and vomiting Acute diarrhea, sometimes with vomiting, is the predominant
E. coli 0157:H7. By Christopher Tong
E. coli 0157:H7 By Christopher Tong The etiologic agent E. coli 0157:H7 have several transmissions that can be spread around to animals and humans. In humans this serotype of E. coli is transmitted to
True Pathogens of the Enterobacteriaceae: Salmonella, Shigella & Yersinia Salmonella
Lec. 6 Oral Microbiology Dr. Chatin True Pathogens of the Enterobacteriaceae: Salmonella, Shigella & Yersinia Salmonella General Characteristics of Salmonella جامعة تكريت كلية طب االسنان Coliform bacilli
Global food trade and emerging foodborne pathogens: The example of STEC O104
Global food trade and emerging foodborne pathogens: The example of STEC O104 Stefano Morabito EU Reference Laboratory for E. coli Dipartimento di Sanità Pubblica Veterinaria e Sicurezza Alimentare Istituto
Escherichia coli diagnostics
Escherichia coli diagnostics Workshop on Whole Genome Sequencing and Analysis, 19-21 Mar. 2018 Learning objective: After this lecture and exercise, you should be able to describe how the CGE methods for
Pathogenic mechanisms in VTEC-associated HUS
Pathogenic mechanisms in VTEC-associated HUS Maurizio Brigotti Department of Experimental Pathology University of Bologna, Italy The main cause of acute renal failure in children under 2-3 years is the
Title: The Operon Encoding SubAB a Novel Cytotoxin is Present in US STEC Isolates ACCEPTED
JCM Accepts, published online ahead of print on February 00 J. Clin. Microbiol. doi:./jcm.000-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
3/18/ Update: STEC Diagnosis and Surveillance in Wisconsin. Objectives. Objectives. Shiga toxin-producing Escherchia coli (STEC)
2014 Update: STEC Diagnosis and Surveillance in Wisconsin Mike Rauch Tim Monson WI State Laboratory of Hygiene Communicable Disease Division WCLN Teleconference March 19, 2014 WISCONSIN STATE LABORATORY
2014 Update: STEC Diagnosis and Surveillance in Wisconsin
2014 Update: STEC Diagnosis and Surveillance in Wisconsin Mike Rauch Tim Monson WI State Laboratory of Hygiene Communicable Disease Division WCLN Teleconference March 19, 2014 WISCONSIN STATE LABORATORY
IPCVA, Buenos Aires - 7 December Infections with Shiga toxin producing E.coli (STEC): emerging issues and reflections on the global food trade
IPCVA, Buenos Aires - 7 December 2012 Infections with Shiga toxin producing E.coli (STEC): emerging issues and reflections on the global food trade Alfredo Caprioli EU Reference Laboratory for Escherichia
coli (EHEC)Hemolysin Genes of a Shiga Toxin 1 (Stx1)- and Stx2Producing, Serotype 0128 Escherichia
Ada Me e log ica Bio tdica Vo l.2000 No. l, 18, 48, ll- The Enterohemorrhagic Escherichia coli (EHEC)Hemolysin Genes of a Shiga Toxin 1 (Stx1)- and Stx2Producing, Serotype 0128 Escherichia coli Strain
Microbial Pathogenesis. How do bacteria cause disease? How do E.coli become pathogens? Commensal flora
Microbial Pathogenesis How do E.coli become pathogens? Commensal flora Acquire genes that cause disease How do bacteria cause disease? 1- Direct toxic effects proteases flesh eating bacteria 2- Activation
Escherichia coli Verotoxigenic Infections
Revision Dates Case Definition Reporting Requirements Epidemiology/Public Health Management March 2011 May 2018 March 2011 Includes O157:H7 Case Definition Confirmed Case Laboratory confirmation of infection
SHIGA-TOXIN PRODUCING ESCHERICHIA COLI STEC Update. Roshan Reporter, MD, MPH Rita Bagby, PS-PHN Leticia Martinez, PS-PHN
SHIGA-TOXIN PRODUCING ESCHERICHIA COLI STEC Update Roshan Reporter, MD, MPH Rita Bagby, PS-PHN Leticia Martinez, PS-PHN Objectives At the conclusion of this presentation the participant should be able
Surveillance and outbreak investigation of Shiga toxin-producing Escherichia coli using whole genome sequencing- time for a change!
Surveillance and outbreak investigation of Shiga toxin-producing Escherichia coli using whole genome sequencing- time for a change! Dr Marie Anne Chattaway Deputy Head STEC Laboratory Gastrointestinal
Comparative genomics of E. coli and Shigella:
Comparative genomics of E. coli and Shigella: Identification and characterization of pathogenic variants based on whole genome sequence analysis David A. Rasko PhD. University of Maryland School of Medicine
Michael Weizenegger Laboratory Group Limbach, Heidelberg, Germany, Dept. of Microbiology and Molecular Genetic
Michael Weizenegger Laboratory Group Limbach, Heidelberg, Germany, Dept. of Microbiology and Molecular Genetic The story of the E. coli out break in Germany A novel strain of Escherichia coli O104.H4 (the
Position Statement Template
Submission Date: 7/6/2005 Committee: Infectious Diseases 05-ID-07 Position Statement Template Title: Revision of the Enterohemorrhagic Escherichia coli (EHEC) condition name to Shiga toxin-producing Escherichia
Improving the Detection of Shiga Toxin- Producing Escherichia coli (STEC)
Improving the Detection of Shiga Toxin- Producing Escherichia coli (STEC) C a ra W i l d e r, P h. D. Te c h n i c a l Wr i t e r, ATC C A u g u s t 1 8, 2016 About ATCC Founded in 1925, ATCC is a non-profit
Bacterial Enteric Pathogens: Clostridium difficile, Salmonella, Shigella, Escherichia coli, and others
GUIDE TO INFECTION CONTROL IN THE HOSPITAL CHAPTER 48 Bacterial Enteric Pathogens: Clostridium difficile, Salmonella, Shigella, Escherichia coli, and others Authors Olivier Vandenberg, MD, PhD Michèle
Zoonosis from the ground
Zoonosis from the ground Alex W. Friedrich Medical Microbiology University Medical Center Groningen alex.friedrich@umcg.nl Reported hospitalisation and case-fatality rates due to zoonoses in confirmed
Ayman Musleh. Osama Hussein, Saba Massimi ... Dr.Anas
14 Ayman Musleh Osama Hussein, Saba Massimi... Dr.Anas Enterobacteriaceae: *General properties: 1. Enterobacteriaceae are moderate-sized (0.3 to 1.0 1.0 to 6.0 μm). 2. non spore-forming. 3. gram-negative
DR. HUDA ABO- ALEES GRAM-NEGATIVE BACILLI THE ENTERICS:
DR. HUDA ABO- ALEES 214-2-15 GRAM-NEGATIVE BACILLI THE ENTERICS: Family Enterobacteriaceae: Genus Escherichia & Genus Klebsiella OBJECTIVES Describe the morphology & physiology for E.coli & Klebsiella
Gram-Negative rods Introduction to
Lec 5 Oral Microbiology Dr. Chatin Gram-Negative rods Introduction to Enterobacteriaceae Characteristics: جامعة تكريت كلية طب االسنان Small gram-negative rods (2-5 by 0.5 microns) Most motile with peritrichous
Gastrointestinal Disease from 2007 to 2014
Data Requested by Amber Erickson, Epidemiologist, North Central Health District Gastrointestinal Disease from 2007 to 2014 North Central Health District Aemon Weaver, Epidemiology Intern, NCHD September
GRAM-NEGATIVE BACILLI THE ENTERICS: Family Enterobacteriaceae: Genus Escherichia & Genus Klebsiella
DR. HUDA ABO- ALEES 214-2-15 Obgectives: GRAM-NEGATIVE BACILLI THE ENTERICS: Family Enterobacteriaceae: Genus Escherichia & Genus Klebsiella Describe the morphology & physiology for E.coli & Klebsiella
2. REVIEW OF LITERATURE
2. REVIEW OF LITERATURE 2.1 PROBLEM STATEMENT Diarrheal diseases are the major cause of death in children under 5 years of age in resourcepoor countries, resulting in approximately 2.5 million deaths each
Pathogens of the Digestive System
Pathogens of the Digestive System Chapter 24 (Pages 625-661) 1. Digestive System Review (Pages 627-629) A. Oral Cavity B. Esophagus C. Stomach D. Small Intestine E. Pancreas F. Liver G. Gall Bladder H.
Nucleotide Polymorphisms within the O-antigen Gene Cluster of Escherichia coli O26,
AEM Accepts, published online ahead of print on 13 July 2012 Appl. Environ. Microbiol. doi:10.1128/aem.01259-12 Copyright 2012, American Society for Microbiology. All Rights Reserved. 1 2 Nucleotide Polymorphisms
Bacteria Pathogen Virulence Primer
A D V A N C E S I N P A T H O G E N R E D U C T I O N Bacteria Pathogen Virulence Primer GREGORY R. SIRAGUSA * Introduction The manner in which bacterial pathogens caused human disease has been and is
Enterohemorrhagic Escherichia coli in Human Infection: In Vivo Evolution of a Bacterial Pathogen
MAJOR ARTICLE Enterohemorrhagic Escherichia coli in Human Infection: In Vivo Evolution of a Bacterial Pathogen Alexander Mellmann, 1,a Martina Bielaszewska, 1,a Lothar B. Zimmerhackl, 4 Rita Prager, 3
Enterovirulent Escherichia coli. Tom Cheasty Laboratory of Enteric Pathogens
Enterovirulent Escherichia coli Tom Cheasty Laboratory of Enteric Pathogens Classes of Enterovirulent E. coli Urinary Tract Septicaemia / Meningitis Enteropathogenic Enteroinvasive Enterotoxigenic Vero
SUMMARY OF FOODBORNE AND WATERBORNE DISEASE CHARACTERISTICS
SUMMARY OF FOODBNE AND WATERBNE DISEASE CHARACTERISTICS BACTERIAL Bacillus cereus Vomiting toxin Diarrheal toxin Brucella species Campylobacter species Clostridium botulinum Clostridium perfringens 1-6
Non-O157 Shiga toxin-producing E. coli: An emerging pathogen of public health importance
Non-O157 Shiga toxin-producing E. coli: An emerging pathogen of public health importance Public Health Ontario Grand Rounds June 17, 2014 Vanessa G. Allen MD MPH Objectives Outline the microbiology and
OPINION 1 / 19. AFSSA Request No SA-0031 Related Request No SA Maisons-Alfort, 27 May 2010
Maisons-Alfort, 27 May 2010 OPINION THE DIRECTOR GENERAL of the French Food Safety Agency on the advisability of revising the definition of pathogenic STEC, specified in AFSSA s Opinion of 15 July 2008.
E. coli O157:H7 Outbreak Associated with Consumption of Unpasteurized Milk, Kentucky, 2014
E. coli O157:H7 Outbreak Associated with Consumption of Unpasteurized Milk, Kentucky, 2014 Association of Food and Drug Officials of the Southern States Fall Educational Conference September 15, 2015 Speakers
Bacterial Enteric Infections Detected by Culture-Independent Diagnostic Tests FoodNet, United States,
Bacterial Enteric Infections Detected by Culture-Independent Diagnostic Tests FoodNet, United States, 2012 2014 Martha Iwamoto, MD 1, Jennifer Y. Huang, MPH 1, Alicia B. Cronquist, MPH 2, Carlota Medus,
Glycosphingolipid-mediated interaction of Shiga toxin with the human endothelium: status quo of receptor research
Glycosphingolipid-mediated interaction of Shiga toxin with the human endothelium: status quo of receptor research Ivan U. Kouzel, Andreas Bauwens, Helge Karch and Johannes Müthing Institute for Hygiene,
Non-O157 Shiga Toxin Producing Escherichia coli in Foods
1721 Journal of Food Protection, Vol. 73, No. 9, 2010, Pages 1721 1736 Copyright G, International Association for Food Protection Review Non-O157 Shiga Toxin Producing Escherichia coli in Foods EMILY C.
Escherichia coli: Great Diversity around a Common Core
Escherichia coli: Great Diversity around a Common Core Author G. Moriel, Danilo, Rosini, Roberto, L. Seib, Kate, Serino, Laura, Pizza, Mariagrazia, Rappuoli, Rino Published 2012 Journal Title mbio DOI
STEC Whole Genome Sequencing Project
STEC Whole Genome Sequencing Project Eija Trees, PhD, DVM Chief, PulseNet Next Generation Subtyping Methods Unit 16 th Annual PulseNet Update Meeting August 29 th, 2012 National Center for Emerging and
Associations between Virulence Factors of Shiga Toxin-Producing Escherichia coli and Disease in Humans
JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1999, p. 497 503 Vol. 37, No. 3 0095-1137/99/$04.00 0 Copyright 1999, American Society for Microbiology. All Rights Reserved. Associations between Virulence Factors
Medical Microbiology Coursework Essay High Class 1 essay. North Germany. This outbreak caused the highest frequency of HUS cases caused by
High Class 1 essay Discuss the new insights in the understanding of Haemolytic Uraemic Syndrome and its worldwide implications following the large scale outbreak of E.Coli O104:H4 diarrhea in Germany 2011
Shigella and salmonella
Sulaimani University College of Pharmacy Microbiology Lec. 9 & 10 Shigella and salmonella Dr. Abdullah Ahmed Hama PhD. Microbiology/Molecular Parasitology abdullah.hama@spu.edu.iq 1 Shigella Shigella species
EFSA Panel on Biological Hazards (BIOHAZ); Scientific Opinion on VTECseropathotype and scientific criteria regarding pathogenicity assessment
Downloaded from orbit.dtu.dk on: Jan 29, 2019 EFSA Panel on Biological Hazards (BIOHAZ); Scientific Opinion on VTEC and scientific criteria regarding pathogenicity assessment EFSA publication; Hald, Tine;
Received 26 May 2005/Returned for modification 1 August 2005/Accepted 7 September 2005
JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2005, p. 6098 6107 Vol. 43, No. 12 0095-1137/05/$08.00 0 doi:10.1128/jcm.43.12.6098 6107.2005 Copyright 2005, American Society for Microbiology. All Rights Reserved.
Robert Tauxe, MD, MPH
Robert Tauxe, MD, MPH Deputy Director, Division of Foodborne, Waterborne and Environmental Diseases National Center for Emerging and Zoonotic Infectious Diseases Centers for Disease Control and Prevention
E. coli O157:H7 shedding in beef cattle. Jane Heller, Geraldine Lammers and Craig McConnel
E. coli O157:H7 shedding in beef cattle Jane Heller, Geraldine Lammers and Craig McConnel Overview Background on E.coli O157:H7 Supershedding of E.coli O157:H7 Overview of collaborative study - MLA Future
One Health in Serbia-Scope of my talk
One Health in Serbia-Scope of my talk Well, I have been living in Serbia little bit shorter of 3 years after my absence of 26 years My prospective of OH in Serbia will be assessed by the comparison of
7th annual workshop of the NRL for E. coli in the EU Rome, 8th -9th November 2012
Prevalence of the 7 major serogroups of enterohemorrhagic Escherichia coli (EHEC) in fresh minced beef in France: A novel real-time PCR strategy for their early detection in food C.Mazuy-Cruchaudet 1,
Epidemiology of Verotoxigenic E. coli O157 in Ireland, 2003 Patricia Garvey and Paul McKeown
Epidemiology of Verotoxigenic E. coli O157 in Ireland, 2003 Patricia Garvey and Paul McKeown National Disease Surveillance Centre 25-27 Middle Gardiner Street, Dublin 1, Ireland Introduction Verotoxigenic
The 12 Most Unwanted Bacteria
The 12 Most Unwanted Bacteria Campylobacter jejuni Most common bacterial cause of diarrhea in the U.S. especially in young children. Raw milk, untreated water, raw and undercooked meat, poultry or shellfish.
Association of Enterohemorrhagic Escherichia coli Hemolysin with Serotypes of Shiga-Like-Toxin-Producing Escherichia coli of Human and Bovine Origins
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Nov. 1998, p. 4134 4141 Vol. 64, No. 11 0099-2240/98/$04.00 0 Copyright 1998, American Society for Microbiology. All Rights Reserved. Association of Enterohemorrhagic
Received 3 September 2010/Accepted 7 January 2011
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2011, p. 2035 2041 Vol. 77, No. 6 0099-2240/11/$12.00 doi:10.1128/aem.02089-10 Copyright 2011, American Society for Microbiology. All Rights Reserved. Detection
Meeting the Challenge of Changing Diagnostic Testing Practices and the Impact on Public Health Surveillance
National Center for Emerging and Zoonotic Infectious Diseases Meeting the Challenge of Changing Diagnostic Testing Practices and the Impact on Public Health Surveillance Aimee Geissler, PhD, MPH FoodNet
EXECUTIVE SUMMARY. The Significance of Non-O157 Shiga Toxinproducing Escherichia coli in Food
EXECUTIVE SUMMARY The Significance of Non-O157 Shiga Toxinproducing Escherichia coli in Food MICHAEL A. Grant, 1 CRAIG Hedberg, 2 ROGER Johnson, 3 Janet Harris, 3 Catherine M. Logue, 4 JIANGHONG Meng,
Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission
Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission Alfredo Caprioli, Stefano Morabito, Hubert Brugère, Eric Oswald To cite this version: Alfredo Caprioli, Stefano
Chapter 1 The Public Health Role of Clinical Laboratories
Chapter 1 The Public Health Role of Clinical Laboratories A. Epidemic Diarrhea The two most common types of epidemic diarrhea in developing countries are watery diarrhea caused by Vibrio cholerae serogroup
New Mexico Emerging Infections Program Overview. Joan Baumbach NM Department of Health September 23, 2016
New Mexico Emerging Infections Program Overview Joan Baumbach NM Department of Health September 23, 2016 Emerging Infections Program History Established in 1995 as population-based, scientific, public
3/08/2012. EHECO104: Lessons for Australia from the German outbreak. E. coli Pathotypes. EHEC Reservoirs & Transmission. EHEC Virulence Markers
E. coli Pathotypes E. coli O157:H7 EHECO104: Lessons for Australia from the German outbreak Rowland Cobbold Senior Lecturer - Veterinary Public Health School of Veterinary Science University of Queensland
Verocytotoxin (vtx)-producing Escherichia coli (VTEC),
FOODBORNE PATHOGENS AND DISEASE Volume 8, Number 3, 2011 ª Mary Ann Liebert, Inc. DOI: 10.1089=fpd.2010.0693 Virulence Profiling and Quantification of Verocytotoxin- Producing Escherichia coli O145:H28
Communicable diseases. Gastrointestinal track infection. Sarkhell Araz MSc. Public health/epidemiology
Communicable diseases Gastrointestinal track infection Sarkhell Araz MSc. Public health/epidemiology Communicable diseases : Refer to diseases that can be transmitted and make people ill. They are caused
Food Microbiology 101
Food Microbiology 101 Nina G. Parkinson NGP Consulting November 6, 2018 Food Safety and Sanitation Conference Summary Microbiological contamination of food Routes of contamination by pathogens Overview
Assessment of Non O157 Shiga Toxin Producing Escherichia coli Infection and Surveillance in Wisconsin
Assessment of Non O157 Shiga Toxin Producing Escherichia coli Infection and Surveillance in Wisconsin Sarah E. Koske, DVM Master of Public Health Candidate Class of 2013 University of Wisconsin Madison
overview Domain: Bacteria Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Enterobacteriales Family: Enterobacteriaceae
E.coli overviwe E. coli is a Gram negative rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms. Most E. coli strains are harmless, but some, such as serotype O157:H7,
FOOD QUALITY AND STANDARDS - Methods of Detection and Characterization of Pathogenic Escherichia Coli - Peter Feng, Nancy Strockbine, Pina Fratamico
METHODS OF DETECTION AND CHARACTERIZATION OF PATHOGENIC ESCHERICHIA COLI Peter Feng Division of Microbiology, U.S. Food and Drug Administration, College Park, MD, USA Nancy Strockbine Centers for Disease
Received 23 February 2011/Returned for modification 28 March 2011/Accepted 5 April 2011
JOURNAL OF CLINICAL MICROBIOLOGY, June 2011, p. 2274 2278 Vol. 49, No. 6 0095-1137/11/$12.00 doi:10.1128/jcm.00386-11 Copyright 2011, American Society for Microbiology. All Rights Reserved. Characterization
Trends in Toxin Profiles of Human Shiga Toxin- Producing Escherichia Coli (STEC) O157 Strains, United States,
Georgia State University ScholarWorks @ Georgia State University Public Health Theses School of Public Health 4-23-2009 Trends in Toxin Profiles of Human Shiga Toxin- Producing Escherichia Coli (STEC)
The Cost-effectiveness of a GI PCR panel in Detecting Necessary to Treat Infections
The Cost-effectiveness of a GI PCR panel in Detecting Necessary to Treat Infections Annie L. Andrews MD, MSCR Annie N. Simpson PhD Kit N. Simpson DrPH Daniel C. Williams MD, MSCR The authors have nothing
Understanding the Public Health Significance of Salmonella. Betsy Booren, Ph.D. Director, Scientific Affairs
Understanding the Public Health Significance of Salmonella Betsy Booren, Ph.D. Director, Scientific Affairs June 18, 2012 2011 Salmonella Outbreaks Ground Beef Salmonella Typhimurium Kosher Broiled Chicken
toxins ISSN
Toxins 2010, 2, 954-962; doi:10.3390/toxins2050954 Review OPEN ACCESS toxins ISSN 2072-6651 www.mdpi.com/journal/toxins The Role of Lymphostatin/EHEC Factor for Adherence-1 in the Pathogenesis of Gram
Hompes Method. Practitioner Training Level II. Lesson Seven Part A DRG Pathogen Plus Interpretation
Hompes Method Practitioner Training Level II Lesson Seven Part A DRG Pathogen Plus Interpretation Health for the People Ltd not for reuse without expressed permission Hompes Method is a trading name of
GENETICALLY ENGINEERED ENTEROPATHOGENIC ESCHERICHIA COLI STRAIN PROTECTS RABBITS AGAINST COLIBACILLOSIS.
Proceedings - th World Rabbit Congress September -1, Puebla, Mexico GENETICALLY ENGINEERED ENTEROPATHOGENIC ESCHERICHIA COLI STRAIN PROTECTS RABBITS AGAINST COLIBACILLOSIS. BOULLIER S., NOUGAYRÈDE J-P.,
EHEC O111 O157
194 16 7 8 16 9 24 (EHEC) (VT) VT RPLA EHEC 39 50 100 RPLA 1/4 1/16 EHEC VT 230 83 80, 70 VT Key words: (enterohemorrhagic Escherichia coli;ehec) 1996 2002 3185 1) EHEC O157 1) O157 (VT) VT RPLA 2) ELISA
Is Whole Genome Sequencing Really Replacing Traditional Microbiology?
Is Whole Genome Sequencing Really Replacing Traditional Microbiology? Peter Gerner-Smidt, MD, DSc Enteric Diseases Laboratory Branch InFORM II Phoenix, AZ, 18 November 2015 National Center for Emerging
Real-Time WGS for Routine Typing, Surveillance, and Outbreak Detection of VTEC. Katrine Grimstrup Joensen
Real-Time WGS for Routine Typing, Surveillance, and Outbreak Detection of VTEC Katrine Grimstrup Joensen Short about me Ph.d project: Application of WGS for Diagnostics, Surveillance and Outbreak Detection
Introduction. Future U.S. initiatives regarding the food safety for fresh produce. FoodNet Partners. FoodNet Partners
Introduction Future U.S. initiatives regarding the food safety for fresh produce This presentation is based upon FDA s testimony about the E. coli outbreaks to the U.S. Congress delivered on November 15,
Dear Healthcare Provider, The information contained here may be very important to your practice. Please take a moment to review this document.
February 2018 Dear Healthcare Provider, The information contained here may be very important to your practice. Please take a moment to review this document. TEST BULLETIN CHLAMYDIA/GONORRHEA SPECIMEN COLLECTION
stx 1c Is the Most Common Shiga Toxin 1 Subtype among Shiga Toxin-Producing Escherichia coli Isolates from Sheep but Not among Isolates from Cattle
JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 2003, p. 926 936 Vol. 41, No. 3 0095-1137/03/$08.00 0 DOI: 10.1128/JCM.41.3.926 936.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved. stx
Top 8 Pathogens. Print this document and study these pathogens. You will be better prepared to challenge the ADVANCED.fst exam.
Top 8 Pathogens The top 8 pathogens outlined in this document often cause foodborne illness in Canada. Take particular note of the bolded/underlined sections, as they are especially important. Print this
ABSTRACT. Non-O157 Shiga toxin-producing Escherichia coli (STEC) are emerging
ABSTRACT Title of Document: NON-O157 SHIGA TOXIN-PRODUCING ESCHERICHIA COLI: PRESENCE IN FOOD, PATHOGENICITY ISLAND AND MOLECULAR EVOLUTION Wenting Ju, Doctor of Philosophy, 2013 Directed By: Professor,
NATIONAL REFERENCE CENTRE FOR SHIGA TOXIN/VEROTOXIN- PRODUCING ESCHERICHIA COLI (NRC STEC/VTEC)
Laboratory of Microbiology and Infection Control, UZ Brussel NATIONAL REFERENCE CENTRE FOR SHIGA TOXIN/VEROTOXIN- PRODUCING ESCHERICHIA COLI (NRC STEC/VTEC) ANNUAL REPORT 2016 K. De Rauw Prof. Dr. D. Piérard
Those Pathogens, What You Should Know
Those Pathogens, What You Should Know Ted F. Beals, MS, MD Short 1 We are at war over our Food Most of us here are convinced that what we eat, and why we choose is our responsibility, not the responsibility
Produce Food Safety. Understand what you want to prevent
Produce Food Safety Dr. Karen Killinger Washington State University Understand what you want to prevent The better we understand how pathogens function, the better we can prevent their survival and growth
EPIDEMIOLOGY OF SHIGA TOXIN-PRODUCING E. COLI (STEC) IN THE FINISHING PIGS AND HUMANS. Marion Tseng
EPIDEMIOLOGY OF SHIGA TOXIN-PRODUCING E. COLI (STEC) IN THE FINISHING PIGS AND HUMANS By Marion Tseng A DISSERTATION Submitted to Michigan State University in partial fulfillment of the requirements for
Real-Time WGS for Routine Typing, Surveillance, and Outbreak Detection of VTEC. Katrine Grimstrup Joensen, Ph.d.student
Real-Time WGS for Routine Typing, Surveillance, and Outbreak Detection of VTEC Katrine Grimstrup Joensen, Ph.d.student Short about me DTU Food Division for Epidemiology and Microbial Genomics (Frank Møller
Gram-negative rods Ferment glucose with acid production Reduce nitrates into nitrites Oxidase negative Facultative anaerobic
Enterobacteriaceae Lecture -17 Dr.Baha,H. AL-Amiedi Ph. D.Microbiology Gram-negative rods Enterobacteriaceae Characters of Enterobacteriaceae EnterobacteriaciaeAll Gram-negative rods Ferment glucose with
#sheet (15) PART.1 Enterobacteriaceae
1Page #sheet (15) PART.1 Enterobacteriaceae Made by: marah al-hiary corrected by: Mohammad al-momani date: 10/11/2016 1 2Page *in this lecture we will talk about a big family which is Enterobacteriaceae:
CORRELATING GENETIC AND PHENOTYPIC CHARACTERISTICS IN AVIAN PATHOGENIC ESCHERICHIA COLI AS A MODEL ENVIRONMENTAL PATHOGEN. Kyle James LeStrange
CORRELATING GENETIC AND PHENOTYPIC CHARACTERISTICS IN AVIAN PATHOGENIC ESCHERICHIA COLI AS A MODEL ENVIRONMENTAL PATHOGEN by Kyle James LeStrange A thesis submitted to the Faculty of the University of
Foodborne Disease in the Region of Peel
Foodborne Disease in the Region of Peel HIGHLIGHTS The incidence of selected foodborne diseases was generally higher in Peel than in Ontario between 1993 and 22. A higher incidence was observed in Peel
University of Wollongong. Research Online
University of Wollongong Research Online Faculty of Engineering and Information Sciences - Papers: Part A Faculty of Engineering and Information Sciences 1993 Molecular epidemiology of escherichia coli