Assured Quality Technically Supported Competitive Costs Customization

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1 Multi-Analyte Flow Assay Kits IL-6 Protein Structure Assured Quality Technically Supported Competitive Costs Customization KRISHGEN BIOSYSTEMS Unit Nos#318/319, Shah & Nahar, Off Dr E Moses Road, Worli, Mumbai Tel: sales@krishgen.com

2 KRISHPLEX ASSAYS EXPLAINED KRISHPLEX multiplex assay technology utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity. With two sizes of beads of 4 and 5 microns and twelve levels of fluorescence intensity in each bead size (4S and 5S), the KRISHPLEX technology can measure up to 24 analytes simultaneously in a single reaction using only 15µL of sample. The bead populations in the reaction are determined using a standard flow cytometer equipped with a blue 488nm and red 635nm laser or with only a 488nm laser. The maximum emission of the bead classification dye is at 700 nm. Similar to the principle of a sandwich ELISA, each bead population, conjugated with a specific capture antibody, traps the protein of interest, such as a cytokine, in the sample. The amount of the analyte captured is detected via a biotinylated antibody against a secondary epitope of the protein, followed by a streptavidin-r-phycoerythrin treatment. The fluorescent intensity of R- phycoerythrin on the beads is quantified on a flow cytometer equipped with a 488nm laser and measured using the PE channel detector. Concentrations of the protein of interest in the samples can be obtained by comparing the fluorescent signals to those of a standard curve generated from a serial dilution of a known concentration of the protein analyte. streptavidin-pe incubation The assay procedure consists of a 60-minute antigen and capture antibody-conjugated bead incubation step, a 30-minute biotinylated detection incubation step and a 20 minute step. Washing the beads after each incubation step via a flow-through filterbottom microplate provides the highest signal-to-noise ratio. Advantages of Bead Based Immunoassay Encoded microparticle arrays exhibits greater sensing flexibility where different capture ligands can be mixed-and-matched at different combinations within Groups. 1

3 Suspended particles with spherical surfaces benefit from faster diffusion and smaller steric hindrance whereas the reaction kinetics on a planar microplate is limited by the flat surface. Encoded microparticle arrays have greater reproducibility due to the use of hundreds to thousands of replicates for each target molecule in the same assay, which allows for high precision measurements Multiplex Immunoassays for Flow: Up to 24 Capture Beads (cab) are differentiated by size and levels of fluorescence intensity Two Bead Sizes 5 Micron (S5) and 4 Micron (S4) Beads 12 Peak Intensities (P1, P2, P3, etc.) for each bead size Each bead is conjugated to a unique antibody (e.g. TNFalpha) that coats the spherical bead Two Different Bead Sizes for Flow: 2

4 Capture Antibody Beads by Flow: PE-Cy5 and APC-like emission (665/30 nm bandpass filter with 488 nm laser and 665/30 nm filter (PE-Cy5 channel) with 640 nm laser and 665 / 30 nm filter (APC channel) Example of 24-plex cab beads analyzed on a conventional flow cytometer equipped with both a 488nm and 640nm laser 3

5 Streptavidin-PE is used as the reporter conjugated to the detection Antibody (dab) is measured with a flow cytometer using a 585/40 nm bandpass filter Reporter Detection in KRISHPLEX Kits Example of 7-plex mouse Th1, Th2, Th17 panel bivarent plots on the S5 bead as measured on a flow cytometer equipped with just a blue laser showing the dab using FL2 (PE) vs the cab using FL3 (PE-Cy5) channels with different Mean Fluorescence Intensities (MFI) on the X-axis R2 IL-1b R1 IL-6 IL-12P70 PE-Cy5 PE-Cy5 IL-8 FSC R1 4 micron beads R2 5 micron beads PE - MFI Gate on R2 (S5 beads) Available Immunoassay Kit Formats Plex-One Kits All available KrishPlex immunoassays are grouped as analytes per group. Each analyte in a group has a unique bead region and can be multiplexed together in any combination. Analytes in different groups may be 4

6 multiplexed together but may have some cross-reactivity because crossreactivity of all the assays across different groups was not validated. We offer custom multiplex assay panel design and development. Please contact us for details including pricing and lead time. Note: Bead set assignment may vary in a panel. Refer to packaging/product inserts and kit labels for details. Each Single-Plex Kit consists of 1 or more user selectable Analyte Kits, a Basic Kit and a sample matrix specific Diluent Kit. Each Analyte Kit contains analyte specific Ab-conjugated beads, detection antibody(ies) and corresponding antigen standard(s). The Basic Kit has species-specific detection antibody diluent, reading buffer, wash buffer, Streptavidin-PE (SAPE), filter plate and plate seals. The Diluent Kit contains sample-type specific Standard Diluent and sample Assay Buffer. Some of the single-plex kits are available in both 96- and 32-test sizes. Cat No Plex-One Kits Human Analyte Kits Group 1 (17 Analytes) KA11122 IFN-gamma KA11128 IL-1beta KA11131 IL-10/CSIF KA11137 IL-12p70 KA11146 IL-17A/CTLA-8 KA11155 IL-2 KA11173 IL-4 KA11179 IL-6 KA11209 TNF-alpha KA12224 IL-8/CXCL8 KA12227 CXCL10/IP-10 KA12233 CCL2/SYCA2/MCP-1 KA12245 CCL5/SCYA5/RANTES KB11152 IL-1RA KB11161 IL-22/IL-TIF KB12473 CCL11/SCYA11/Eotaxin KB12497 CCL7/SYCA7/MCP-3/MARC HG10007 Standard Mix - Human Group 1 Panel A, 7-Plex HG10011 Standard Mix - Human Group 1 Panel B Group 2 (20 Analytes) KA11110 G-CSF/CSF-3 KA11113 GM-CSF/CSF-2 KA11119 IFN-alpha 2 KA11125 IL-1alpha/IL-1F1 KA11134 IL-11 KA11140 IL-12/IL-23p40 KA11143 IL-13 KA11149 IL-17F KA11158 IL-21 KA11170 IL-3 5

7 Cat No Plex-One Kits Human Analyte Kits Group 2 (20 Analytes) KA11176 IL-5 KA11182 IL-7 KA11215 VEGF-A KB11405 IL-20/IL-10D KB11410 IL-28A/IFNlambda 2 KB11413 IL-29/IFNlambda 1 KB11419 IL-33 KB11428 M-CSF/CSF1 KB11434 MPO KB11461 TSLP HG2009A Standard Mix - Human Group 2 Panel A, 10-Plex HG20010 Standard Mix - Human Group 2 Panel B, 10-Plex Group 3 (20 Analytes) KA11185 IL-9 KA11212 TNF-beta/TNFSF1/LTA KA12221 CX3CL1/Fractalkine KA12230 CXCL11/I-TAC KA12239 CCL3/SCYA3/MIP-1alpha KA12242 CCL4/MIP-1beta KB11164 IL-23 KB11167 IL-27 KB11218 VEGF-D KB11398 IL-15 KB11401 IL-16/LCF KB11402 IL-17C KB11406 IL-24 KB11416 IL-31 KB11417 IL-32 KB11422 IL-34 KB11427 LIF KB11437 OSM/Oncostatin M KB11443 SCF/MGF/KIT-Ligand KB11452 TIM-1/HAVCR/KIM-1 HG30008 Standard Mix - Human Group 3 Panel A, 8-Plex HG3012B Standard Mix - Human Group 3 Panel B, 12-Plex Group 4 (18 Analytes) KA11107 FGF basic/fgf-2/hbgh-2 KB11101 beta-ngf/ngfb KB11104 EGF KB11188 OPG/Osteoprotegerin/TNFRSF11B KB11191 OPN/Osteopontin/BNSP KB11194 PDGF-AB * KB11203 Resistin/ADSF/XCP1 KB11362 BDNF KB11381 GASP-1/WFIKKNRP KB11389 IFN-beta KB11431 MIF/MMIF/GIF KB11446 Survivin/BIRC5 KB11449 TGF-alpha/TGF-type I/ETGF KB11455 THPO/Thrombopoietin/TPO 6

8 Cat No Plex-One Kits Human Analyte Kits Group 4 (18 Analytes) KB11523 VEGF-C KB14314 Insulin KB14317 Leptin/LEP KB16641 ANGPT-1/Angiopoietin-1 HG4009A Standard Mix - Human Group 4 Panel A, 8-Plex HG40010 Standard Mix - Human Group 4 Panel B, 10-Plex Group 5 (18 Analytes) KA13254 E-selectin/ELAM-1/sCD62E ** KA13257 TNFSF6/sCD178/sCD95L/sFasL KA13263 ICAM-1/sCD54 KA13278 CD14/sCD14 KA13293 TNFSF8/sCD154/sCD40L * KA13305 TNFRSF1A/sCD120a/sTNFRI KA13311 VCAM-1/sCD106 KB13200 TNFSF11/sCD254/RANKL KB13251 Endoglin/sCD105 KB13269 IL-2RA/sCD25 KB13290 PECAM-1/sCD31 KB13299 VEGFR2/Flk-1/sCD309/KDR KB13345 TNFRSF9/sCD137/4-1BB KB13366 BlyS/BAFF/sCD257/TNFSF13B KB13367 TNFSF14/sCD258/sLIGHT KB13368 TNFSF8/sCD153/sCD30L KB13369 TNFRSF10c/sCD263/TRAIL-R3 KB13387 E-Cadherin/CDH1/sCD324 HG50008 Standard Mix - Human Group 5 Panel A, 8-Plex HG50010 Standard Mix - Human Group 5 Panel B, 10-Plex Group 6 (19 Analytes) KA13284 IL-1R2/sCD121b KA13308 TNFRSF1B/sCD120b/sTNFR2/sTNFRII KB12464 CCL21/SCYA21/6Ckine KB12465 CXCL14/BRAK KB12467 CXCL13/BCA-1 KB12468 CCL27/SCYA27/CTACK KB12469 CCL28/SCYA28/MEC KB12470 CXCL5/ENA-78 KB12471 CXCL16/SCYB16 KB12476 CCL24/SCYA24/Eotaxin-2/MPIF-2 KB12500 CCL13/SCYA13/MCP-4 KB12503 CCL22/SCYA22/MDC KB12506 CCL15/SCYA15/MIP-1δ/MIP-5 KB12509 CCL20/SCYA20/MIP-3α KB12512 CCL19/SCYA19/MIP-3β KB13527 Gp130/sCD130 KB13536 IL-6Rα/IL-6R alpha/scd126 KB14557 IGFBP-1 KB14575 IGFBP-7 HG61007 Standard Mix - Human Group 6, Panel A, 7-Plex HG62013 Standard Mix - Human Group 6, Panel B, 12-Plex 7

9 Cat No Plex-One Kits Human Analyte Kits Group 7 (15 Analytes) KB12479 CCL26/SCYA26/Eotaxin-3 KB12482 CXCL6/GCP-2 KB12485 CXCL1/MGSA/GROα KB12488 CCL14/SCYA14/HCC-1 KB12489 CCL16/SCYA16/HCC-4 KB12491 CCL1/SCYA1/I-309 KB12494 CCL8/SCYA8/MCP-2 KB12513 CCL23/SCYA23/MPIF-1 KB12515 CXCL7/NAP-2 KB12516 CCL18/SCYA18/PARC/MIP-4 KB12517 CXCL4/PF4 KB12518 CXCL12/SDF-1 KB12521 CCL17/SCYA17/TARC KB12522 CCL25/SCYA25/TECK KB13385 CD279/sPD-1/sCD279 HG71008 Standard Mix - Human Group 7, Panel A, 8-Plex HG72006 Standard Mix - Human Group 7, Panel B, 7-Plex Group 8 (18 Analytes) KA17332 Granzyme B/GZYMB/CTL1 KB11374 FGF-1/FGF-acidic/HBGF-1 KB11377 FGF-19 KB11380 Flt-3 Ligand/Flt-3L/Flk-2L KB11383 GDF-15/MIC-1/PTGFB KB13542 RAGE/AGER/sRAGE KB14563 IGFBP-3 KB14569 IGFBP-5 KB14572 IGFBP-6 KB15323 TIMP-1 KB15581 BMP-2 KB15584 BMP-4 KB15605 Cystatin C KB15638 TIMP-2 KB16659 Lipocalin-2/LCN2/NGAL KB16665 PAPP-A KB17696 SLP1/ALP/BLPI KB36519 ST2/IL-1R4/IL-1RL1 HG81007 Standard Mix - Human Group 8, Panel A, 7-Plex HG82011 Standard Mix - Human Group 8, Panel B, 11-Plex Group 9 (17 Analytes) KA13260 TNFRSF6/sCD95/sFasR KA13266 ICAM-3/sCD50 KA13272 L-selectin/LECAM1/sCD62L KA13275 P-selectin/LECAM-3/sCD62P KA13281 IL-1RI/sCD121a KA13296 VEGFR1/Flt-1 * KB13330 TNFRSF5/sCD40 KB13331 CEACAM-1/sCD66a KB13336 EPO-R/Erythropoietin Receptor KB13357 ALCAM/sCD166 KB13365 SLAMF4/2B4/NAIL/sCD244 8

10 Cat No KB13379 KB13383 KB13384 KB13389 KB13395 KB13539 HG9008A HG9009B Plex-One Kits Human Analyte Kits Group 9 (17 Analytes) TNFRSF14/sCD270/sHVEM PDCD1LG2/PD-L2/sCD273 PD-L1/sCD274/sB7-H1 EpCAM/MK-1/TROP1/sCD326 SLAMF8/BLAME/SBBI42/sCD353 NCAM-1/sCD56 Standard Mix - Human Group 9, Panel A, 8-Plex Standard Mix - Human Group 9, Panel B, 9-Plex Group 10 (16 Analytes) KB11192 PDGF-AA * KB11197 PDGF-BB * KB11326 KGF/FGF-7 KB11358 Betacellulin/BTC KB11364 FGF-4 KB11365 CNTF KB11379 Follistatin/FST KB11404 IL-19 KB11432 Midkine/MK1/NEGF-2 KB11435 NRG1-beta1/HRG1-beta1 KB11442 PIGF/PIG-F KB11458 TNFSF10/sCD253/TRAIL KB12492 Lymphotactin/XCL1 KB13375 TNFRSF13B/sCD267/TACI KB14578 NOV/CCN3/IGFBP-9 KB16660 MIA/CD-RAP HG1009A Standard Mix - Human Group 10, Panel A, 9-Plex HG1007B Standard Mix - Human Group 10, Panel B, 7-Plex Additional Plex-One Kits KA12236 CXCL9/SCYB9/MIG KA13248 Adiponectin KA16326 CRP/C-Reactive Protein KA17329 Granzyme A/GZYMA/CTL3A KA17355 Amphiregulin KB11116 HGF/Hepatopoietin A KB11357 Activin A KB11359 TNFSF13/sCD256/APRIL KB11360 GDNF/ATF-1 KB11372 Artemin/ARTN/Neublastin KB11393 FGF-9/HBGF-9/GAF KB11407 IL-25/IL-17E KB11423 Galectin-1/Galaptin KB11426 IL-18 KB11453 LAP (TGF-beta1)/Latent TGF-beta1 KB11465 AFP/Alpha-fetoprotein KB12507 CXCL2/GROβ/MIP-2α/GRO2 KB12508 EG-VEGF/PROK1/PK1 KB13355 CD163/M130/sCD163 KB13377 CD269/BCMA/TNFRSF17/sCD269 KB13530 EGFR/ErbB1 KB14328 Procalcitonin/PCT 9

11 Cat No Plex-One Kits Human Analyte Kits Additional Plex-One Kits KB14560 IGFBP-2 KB15593 CA-125/MUC16 KB15596 CA15-3 KB15599 CA19-9 KB15602 CA 50 KB15603 CEA/CD66e/CEACAM5 KB15608 Endostatin KB15609 TWEAK/TNFSF12/Apo3 Ligand KB15611 MMP-1/CLGN (Total) KB15620 MMP-13/Collagenase 3 (Total) KB15626 MMP-3/Stomelysin-1 (Total) KB15629 MMP-7/PUMP-1 (Total) KB15632 MMP-8/Collagenase 2 (Total) KB15635 MMP-9/GELB (Total) KB17335 IgA KB17338 IgE KB17341 IgG1 KB17344 IgG2 KB17347 IgG3 KB17350 IgG4 KB17376 Pro-BDNF KB17490 Klotho/KLA/KL KB17686 LL37/CAMP/CAP18 KB17697 BIGH3/TGFBI/βIG-H3 KB17728 Ferritin KB Keratin 19/KRT19/CK-19 32test Human Analyte Kits Group 1 (17 Analytes) KA11122T IFN-gamma KA11128T IL-1beta KA11131T IL-10/CSIF KA11137T IL-12p70 KA11146T IL-17A/CTLA-8 KA11152T IL-1RA KA11155T IL-2 KA11161T IL-22/IL-TIF KA11173T IL-4 KA11179T IL-6 KA11209T TNF-alpha KA12224T IL-8/CXCL8 KA12227T CXCL10/IP-10 KA12233T CCL2/SCYA2/MCP-1 KA12245T CCL5/SCYA5/RANTES KA12473T CCL11/SCYA11/Eotaxin KA12497T CCL7/SCYA7/MCP-3/MARC Group 2 (17 of the 20 analytes available as 32 tests) KA11110T G-CSF/CSF-3 KA11113T GM-CSF/CSF-2 KA11119T IFN-alpha 2 KA11125T IL-1alpha/IL-1F1 10

12 Cat No KA11134T KA11140T KA11143T KA11149T KA11158T KA11170T KA11176T KA11182T KA11215T KA11405T KA11419T KA11428T KA11461T Plex-One Kits Human Analyte Kits 32 Test Human Analyte Kits IL-11 IL-12/IL-23p40 IL-13 IL-17F IL-21 IL-3 IL-5 IL-7 VEGF-A IL-20/IL-10D IL-33 M-CSF TSLP Cat No Mouse Analyte Kits Group 1 (16 Analytes) KA21110 IFN-gamma KA21113 IL-1alpha/IL-1F1 KA21119 IL-10/CSIF KA21122 IL-12p70 KA21134 IL-17A/CTLA-8 KA21140 IL-2 KA21161 IL-4 KA21167 IL-6 KA21176 TNF-alpha KA22179 CXCL1/KC/CINC1/KC/Gro alpha KA22185 MCP-1/JE/CCL2 KA22191 CXCL9/MIG KA22200 CCL5/SCYA5/RANTES KB22182 IP-10/CXCL10 KB22188 CCL7/SCYA7/MCP-3/MARC KB22296 CCL11/SCYA11/Eotaxin MG10007 Standard Mix - Mouse Group 1 Panel A, 7-Plex MG10009 Standard Mix - Mouse Group 1 Panel B, 9-Plex Group 2 (18 Analytes) KA21101 G-CSF/CSF-3 KA21104 GM-CSF/CSF-2 KA21116 IL-1beta/IL-1F2 KA21125 IL-12/IL-23p40 KA21128 IL-13 KA21137 IL-17F KA21143 IL-21 KA21158 IL-3 KA21164 IL-5 KA22194 MIP-1 alpha/ccl3 KA22197 MIP-1beta/CCL4 KB21131 IL-15 KB21146 IL-22 KB21155 IL-28A/B/IFNlambda 2/3 11

13 Cat No KB KB KB KB MG2009A KA21170 KB21149 KB21221 KB21239 KB21245 KB21254 KB21257 KB21272 KB22281 KB22289 KB22291 KB22299 KB22308 KB22311 KB23329 KB23338 KB23419 KB23437 MG3008A MG3010B KB21212 KB21216 KB21218 KB21226 KB21230 KB21236 KB21237 KB21242 KB21248 KB21249 KB21264 KB21276 KB22278 KB22287 KB22305 KB22314 KB22320 KB25491 MG4007A MG4011B KA23203 KA23206 KA23209 Mouse Analyte Kits Group 2 (18 Analytes) IL-7 M-CSF/CSF-1 OSM/Oncostatin M TSLP Standard Mix - Mouse Group 2 Panel A, 10-Plex Group 3 (18 Analytes) IL-9 IL-23p19 BTC/Betacellulin IL-1RA IL-25/IL-17E Leptin/LEP LIF VEGF CCL12/SCYA12/MCP-5 CCL27/SCYA27/CTACK CXCL16/SCYB16 CXCL5/LIX/GCP2 MIP-2/GRO beta/cinc3 CCL20/SCYA20/MIP-3α TNFSF13B/BlyS/BAF/sCD257 IL-7R/IL-7Rα/sCD127 TNFSF11/CD254/RANKL IL-2Rα/Ly43/sCD25 Standard Mix - Mouse Group 3 Panel A, 8-Plex Standard Mix - Mouse Group 3 Panel B, 10-Plex Group 4 (18 Analytes) Activin A FGF-9/HBGF-9/GAF BRAK/CXCL14/MIP-2γ/BMAC BDNF HGF/HPTA/SF IL-11 FGF-1/FGF-acidic/HBGF-1 IL-20/IL-10D IL-33/NFHEV/DVS27 FGF-2/FGF basic/hbgh-2 OPG/Osteoprotegerin/TNFRSF11B CCL21/SCYA21/Exodus-2 /6Ckine BLC/CXCL13 MIP-1γ/CCL9/CCL10/MRP-2/CCF18 MDC/CCL22/ABCD-1/STCP-1 MIP-3β/CCL19/ELC TARC/CCL17 TIMP-1 Standard Mix - Mouse Group 4 Panel A, 7-Plex Standard Mix - Mouse Group 4 Panel B, 11-Plex Group 5 (19 Analytes) CD62E/sE-selectin/ELAM1 CD62L/sL-selectin/LECAM1 CD62P/sP-selectin/ LECAM-3 12

14 Cat No KB23330 KB23331 KB23332 KB23334 KB23335 KB23353 KB23357 KB23362 KB23371 KB23386 KB23408 KB23422 KB23446 KB23449 KB23458 KB23461 MG5009A MG5010B KB21258 KB21261 KB22282 KB22290 KB22325 KB23428 KB25489 KB26494 KA22296T KA22179T KA21110T KA21119T KA21122T KA21134T KA21113T KA21140T KA21161T KA21167T KA22182T KA22185T KA22188T KA22191T KA22200T KA21176T KA21104T KA21125T KA21128T KA21131T KA21116T Mouse Analyte Kits Group 5 (19 Analytes) CD10/Neprilysin/sNEP/CALLA/sCD10 CD14/sCD14 CD105/Endoglin/sCD105 CD106/sVCAM-1 CD119/sIFNγR1/IFNγRa CD153/sCD30L/TNFSF8 CD154/sCD40L/TRAP/TNFSF5 CD266/sTWEAK-R/Fn14/TNFRSF12a CD27/sTNFRSF7/sCD27 CD309/sVEGFR2/KDR/Flk-1 CD54/sICAM-1 CD121a/sIL-1R1 RAGE/sRAGE/AGER VEGFR1/Flt-1/sVEGFR1 CD120a/sTNFRI/TNFRSF1A CD120b/sTNFRII/TNFRSF1B Standard Mix - Mouse Group 5 Panel A, 9-Plex Standard Mix - Mouse Group 5 Panel B, 10-Plex Single-Plex Kits EGF beta-ngf/ngfb CCL8/MCP-2 CCL28/MEC/CCK-1 Eotaxin-2/CCL24/MPIF-2 CD30/TNFRSF8/sCD30 ANGPT-1/Angiopoietin-1 CRP 32test Mouse Analyte Kits Group 1 (16 Analytes) Eotaxin/CCL11 Gro alpha/kc/cinc1/cxcl1 IFNgamma IL-10/CSIF IL-12p70 IL-17A/CTLA-8 IL-1alpha/IL-1F1 IL-2 IL-4 IL-6 IP-10/CXCL10 MCP-1/JE/CCL2 MCP-3/MARC/CCL7 MIG/CXCL9 RANTES/CCL5 TNFalpha Group 2 (9 of the 18 analytes available as 32 tests) GM-CSF/CSF-2 IL-12/IL-23p40 IL-13 IL-15 IL-1beta/IL-1F2 13

15 Cat No KA21143T KA21146T KA21164T KA22194T KB32217 KB31153 KB31157 KB32225 KA31101 KA31109 KB31173 KB31177 KB31113 KA31105 KB31165 KA31117 KA31121 KB31185 KA31125 KB32229 KB32237 KB32241 KA31129 RG10007 RG10012 KB31141 KB31138 KB31169 KB31178 KB31189 KB32257 KB31133 KB32233 KB32245 KB32246 KB33269 KB32249 KB31201 RG20006 RG20007 KB31190 KB35313 KB32218 KB31193 KB31186 KB41130 KB41090 Rat Analyte Kits Group 1 (19 Analytes) IL-21 IL-22 IL-5 MIP-1 alpha/ccl3 Eotaxin/CCL11 G-CSF/CSF-3 GM-CSF/CSF-2 GRO alpha/kc/cinc1 IFNgamma IL-10 IL-12p70 IL-13 IL-17A/CTLA-8 IL-1alpha/IL-1F1 IL-1beta/IL-1F2 IL-2 IL-4 IL-5 IL-6 IP-10/CXCL10 MIP-1alpha/CCL3 MIP-2/GRO beta/cinc3 TNFalpha Standard Mix - Rat Group 1 Panel A, 7-Plex Standard Mix - Rat Group 1 Panel B, 12-Plex Group 2 (13 Analytes) beta-ngf/ngfb CD254/RANKL/TNFSF11 IL-12p40 IL-15 Leptin LIX/GCP2/CXCL5 MCP-1/JE/CCL2 MCP-3/MARC/CCL7 RANTES/CCL5 SDF-1/CXCL12 sicam-1/scd54 svcam-1/scd106 VEGF Standard Mix - Rat Group 2 Panel A, 6-Plex Standard Mix - Rat Group 2 Panel B, 7-Plex Additional Plex-One Kits PDGF-AB TIMP-1 FGF-2/FGF basic/hbgh-2 PDGF-BB BDNF NHP Analyte Kits Group 1 (16 Analytes) IL-12p70 Eotaxin/CCL11 14

16 Cat No KA41050 KB41120 KB41145 KB41115 KB41155 KA41055 KA41060 KA41070 KB41160 KB41165 KB41175 KB41180 KB41185 KA41075 NG10007 NG10011 KB41085 KB41095 KA41065 KB41170 KB42245 KB42220 KB41195 Cat No KB51128 KB51113 KB51116 KB51119 KB51122 KB51125 KB51117 KB51134 KB51110 KB51131 KG10010 KB KB NHP Analyte Kits Group 1 (16 Analytes) IFNgamma IL-10/CSIF IL-17A/CTLA-8 IL-1beta IL-1RA IL-2 IL-4 IL-6 IL-8/CXCL8 IP-10/CXCL10 MCP-1/JE/CCL2 MCP-3/MARC/CCL7 RANTES/CCL5 TNFalpha Standard Mix - NHP Group 1 Panel A, 7-Plex Standard Mix - NHP Group 1 Panel B Additional Plex-One Kits beta-ngf/ngfb FGF basic/fgf-2/hbgh-2 IL-5 MIG/CXCL9 SDF-1 TGFalpha/TGF-type I/ETGF VEGF-A Canine Analyte Kits Group 1 (10 Analytes) IFNγ IL-2 IL-6 IL-8/CXCL8 IL-10/CSIF IL-12p40 IL-17A MCP-1/JE/CCL2 SCF/MGF/KITLG Standard Mix - Canine Group 1 Panel A, 10-Plex Additional Plex-One Kits VEGF Insulin Premixed Multiplex Kits Each Premixed Multiplex Kit has a predefined multiplex panel with premixed antibody-conjugated beads, antigens and detection antibodies. Except the Reading Buffer (10x) and Wash Buffer (10x), all the reagents are supplied as ready-to-use. Most of the premixed multiplex kits are available in both 96- and 32-test sizes. Note: Bead set assignment may vary in a panel. Refer to packaging/product inserts and kit labels for details. 15

17 Cat No Premixed Human Multiplex Kits KC11031 Human T Helper Cytokine 3-Plex Panel 1 1 x 96 wells IL-9, IL-10, HG10031 Standard Mix - Human T Helper Cytokine 3-Plex Panel 1 1 no Lyophilized premixed 3 Antigens (IL-9, IL-10, ) KC11032 Human T Helper Cytokine 3-Plex Panel 2 1 x 96 wells IL-6, IL-10, HG10032 Standard Mix - Human T Helper Cytokine 3-Plex Panel 2 1 no Lyophilized premixed 3 Antigens (IL-6, IL-10, ) KC11033 Human T Helper Cytokine 3-Plex Panel 3 1 x 96 wells IFNγ, IL-10, HG10033 Standard Mix - Human T Helper Cytokine 3-Plex Panel 3 1 no Lyophilized premixed 3 Antigens (IFNγ, IL-10, ) KC11041 Human T Helper Cytokine 4-Plex Panel 1 1 x 96 wells IFNγ, IL-4, IL-6, HG10041 Standard Mix - Human T Helper Cytokine 4-Plex Panel 1 1 no Lyophilized premixed 4 Antigens (IFNγ, IL-4, IL-6, ) KC11042 Human T Helper Cytokine 4-Plex Panel 2 1 x 96 wells IFNγ, IL-4, IL-10, HG10042 Standard Mix - Human T Helper Cytokine 4-Plex Panel 2 1 no Lyophilized premixed 4 Antigens (IFNγ, IL-4, IL-10, ) KC11043 Human T Helper Cytokine 4-Plex Panel 3 1 x 96 wells IFNγ, IL-10,, TNFβ HG10043 Standard Mix - Human T Helper Cytokine 4-Plex Panel 3 1 no Lyophilized premixed 4 Antigens (IFNγ, IL-10,, TNFβ) KC11014 Human T Helper Cytokine 14-Plex 1 x 96 wells GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL- 6, IL-9, IL-10, IL-12p70, IL-13, IL- 17A, IL-21, IL-22 and TNF-α HG10014 Standard Mix - Human T Helper Cytokine 14-Plex 1 no Lyophilized premixed 14 Antigens (GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL- 6, IL-9, IL-10, IL-12p70, IL-13, IL- 17A, IL-21, IL-22 and TNF-α) KC11051 Human Th1/Th2 5-Plex Panel 1 1 x 96 wells IFNγ, IL-2, IL-6, IL-10, HG10051 Standard Mix - Human T Helper Cytokine 5-Plex Panel 1 1 no Lyophilized premixed 5 Antigens (IFNγ, IL-2, IL-6, IL-10, ) KC11052 Human Th1/Th2 5-Plex Panel 2 1 x 96 wells IFNγ, IL-4, IL-6, IL-10, HG10052 Standard Mix - Human T Helper Cytokine 5-Plex Panel 1 1 no Lyophilized premixed 5 Antigens (IFNγ, IL-4, IL-6, IL-10, ) KC11053 Human Th1/Th2 5-Plex Panel 3 1 x 96 wells IFNγ, IL-4, IL-5, IL-10, HG10053 Standard Mix - Human T Helper Cytokine 5-Plex Panel 3 1 no Lyophilized premixed 5 Antigens (IFNγ, IL-4, IL-5, IL-10, ) KC11061 Human Th1/Th2 6-Plex Panel 1 1 x 96 wells IFNγ, IL-2, IL-4, IL-6, IL-10, KC11061T Human Th1/Th2 6-Plex Panel 1 1 x 32 wells IFNγ, IL-2, IL-4, IL-6, IL-10, HG10061 Standard Mix - Human Th1/Th2 6-Plex Panel 1 1 no Lyophilized premixed 6 Antigens (IFNγ, IL-2, IL-4, IL-6, IL-10, ) KC11062 Human Th1/Th2 6-Plex Panel 2 1 x 96 wells IFNγ, IL-2, IL-4, IL-5, IL-10, KC11062T Human Th1/Th2 6-Plex Panel 2 1 x 32 wells IFNγ, IL-2, IL-4, IL-5, IL-10, HG10062 Standard Mix - Human Th1/Th2 6-Plex Panel 2 1 no Lyophilized premixed 6 Antigens (IFNγ, IL-2, IL-4, IL-5, IL-10, ) KC11071 Human Th1/Th2 7-Plex Panel 1 x 96 wells IFNγ, IL-2, IL-4, IL-5, IL-6, IL-10, HG10071 Standard Mix - Human Th1/Th2 7-Plex Panel, 1 1 no Lyophilized premixed 7 Antigens (IFNγ, IL-2, IL-4, IL-5, IL-6, IL-10, ) KC10010 Human Th1/Th2 10-plex 1 x 96 wells IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-8, IL-10, IL-12p70, TNFalpha KC10010T Human Th1/Th2 10-plex 1 32 wells IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-8, IL-10, IL-12p70, TNFalpha HG1010 Standard Mix Set - Human TH1/Th2 10- Plex 1 no Lyophilized premixed 10 Antigens (IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-8, IL-10, IL-12p70, TNFalpha), 1 set of 2 vials 16

18 Cat No Premixed Human Multiplex Kits KC10011 Human Th1/Th2 11-plex 1 x 96 wells GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL- 5, IL-6, IL-9, IL-10, IL-12p70, HG19011 Standard Mix Set - Human TH1/Th2 11- Plex 1 no Lyophilized premixed 11 Antigens (GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, ), 1 set of 2 vials KC11107 Human Th1/Th2/Th17 7-plex Panel 1 x 96 wells IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, KC11107T Human Th1/Th2/Th17 7-plex Panel 1 x 32 wells IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, HG10007 Standard Mix - Human Group 1 Panel A, 7-Plex 1 no Lyophilized premixed 7 Antigens (IFNgamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFalpha) KC11111 Human Th1/Th2/Th17 11-plex 1 x 96 wells IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-10, IL-12p70, IL-17A, TNFalpha, TNFbeta HG19111 Standard Mix Set - Human TH1/Th2/Th17 11-plex 1 no Lyophilized premixed 11 Antigens (IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-10, IL-12p70, IL-17A, TNFalpha, TNFbeta), 1 set of 2 vials KC11112 Human Th1/Th2/Th17 12-plex 1 x 96 wells IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-8, IL-10, IL-12p70, IL- 17A, TNFalpha, TNFbeta HG19112 Standard Mix Set - Human TH1/Th2/Th17 12-plex 1 no Lyophilized premixed 12 Antigens (IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-8, IL-10, IL-12p70, IL- 17A, TNFalpha, TNFbeta) KC11114 Human Th1/Th2/Th17 14-plex 1 x 96 wells IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-8, IL-10, IL-12p70, IL- 17A, IL-17F, IL-22, TNFalpha, TNFbeta KC1114T Human Th1/Th2/Th17 14-plex 1 x 32 wells IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-8, IL-10, IL-12p70, IL- 17A, IL-17F, IL-22, TNFalpha, TNFbeta HG19114 Standard Mix Set - Human TH1/Th2/Th17 14-plex 1 no Lyophilized premixed 14 Antigens (IFNgamma, IL-1beta, IL-2, IL-4, IL- 5, IL-6, IL-8, IL-10, IL-12p70, IL- 17A, IL-17F, IL-22, TNFalpha, TNFbeta), 1 set of 2 vials KC11118 Human Th1/Th2/Th17 18-plex 1 x 96 wells GM-CSF, IFNgamma, IL-1beta, IL- 2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, IL- 33, TNFalpha, TNFbeta, TSLP KC11118T Human Th1/Th2/Th17 18-plex 1 x 32 wells GM-CSF, IFNgamma, IL-1beta, IL- 2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, IL- 33, TNFalpha, TNFbeta, TSLP HG19118 Standard Mix Set - Human TH1/Th2/Th17 18-plex 1 no Lyophilized premixed 18 Antigens (GM-CSF, IFNgamma, IL-1beta, IL- 2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, IL- 33, TNFalpha, TNFbeta, TSLP), 1 set of 3 vials KC12206 Human Inflammation 6-Plex Panel 1 x 96 wells IL-1β, IL-6, IL-8, IL-10, IL-12p70, KC12206T Human Inflammation 6-Plex Panel 1 x 32 wells IL-1β, IL-6, IL-8, IL-10, IL-12p70, HG19106 Standard Mix - Human Inflammation 6- Plex 1 no Lyophilized premixed 6 Ags (IL-1β, IL-6, IL-8, IL-10, IL-12p70, ) 17

19 Cat No Premixed Human Multiplex Kits KC12207 Human Inflammation 7-Plex Panel 1 x 96 wells IL-1β, IL-1RA, IL-6, IL-8, IL-10, IL- 12p70, KC12207T Human Inflammation 7-Plex Panel 1 x 32 wells IL-1β, IL-1RA, IL-6, IL-8, IL-10, IL- 12p70, HG1107 Premixed Human Inflammation 7-Plex lyophilized standards 1 no IL-1b, IL-1RA, IL-8, IL-6, IL-10, TNFalpha, IL-12p70 KC12211 Human Inflammation 11-Plex 1 x 96 wells IFNgamma, IL-1alpha, IL-1beta, IL- 6, IL-8, IL-10, IL-12p70, IL-27, IP- 10, MCP-1, TNFalpha KC12211T Human Inflammation 11-Plex 1 x 32 wells IFNgamma, IL-1alpha, IL-1beta, IL- 6, IL-8, IL-10, IL-12p70, IL-27, IP- 10, MCP-1, TNFalpha HG19211 Standard Mix Set - Human Inflammation 11-Plex 1 no Lyophilized premixed 11 Antigens (IFNgamma, IL-1alpha, IL-1beta, IL-6, IL-8, IL-10, IL-12p70, IL-27, IP-10, MCP-1, TNFalpha), 1 set of 2 vials KC12216 Human Inflammation 16-Plex 1 x 96 wells IFNgamma, IL-1alpha, IL-1beta, IL- 6, IL-8, IL-10, IL-12p70, IL-13, IL- 17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1alpha, and TNFalpha KC12216T Human Inflammation 16-Plex 1 x 32 wells IFNgamma, IL-1alpha, IL-1beta, IL- 6, IL-8, IL-10, IL-12p70, IL-13, IL- 17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1alpha, and TNFalpha HG19216 Standard Mix Set - Human Inflammation 16-Plex 1 no Lyophilized premixed 18 Antigens (IFNgamma, IL-1alpha, IL-1beta, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL- 17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1alpha, and TNFalpha), 1 set of 3 vials KC12218 Human Inflammation 18-Plex 1 x 96 wells IFNgamma, IL-1alpha, IL-1beta, IL- 4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1alpha, MIP-1beta, and TNFalpha KC12218T Human Inflammation 18-Plex 1 x 32 wells IFNgamma, IL-1alpha, IL-1beta, IL- 4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1alpha, MIP-1beta, and TNFalpha HG19218 Standard Mix Set - Human Inflammation 18-Plex 1 no Lyophilized premixed 18 Antigens (IFNgamma, IL-1alpha, IL-1beta, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL- 13, IL-17A, IL-27, IL-31, IL-33, IP- 10, MCP-1, MIP-1alpha, MIP-1beta, and TNFalpha), 1 set of 3 vials KC13306 Human Chemokine 6-Plex Panel 1 x 96 wells Eotaxin, IL-8, IP-10, MCP-1, MCP- 3, RANTES KC13306T Human Chemokine 6-Plex Panel 1 x 32 wells Eotaxin, IL-8, IP-10, MCP-1, MCP- 3, RANTES HG19306 KC13371 HG19371 KC13372 Premixed Human Chemokine 6-Plex lyophilized standards Human Inflammatory Chemokine 7-Plex Panel 1 Standard Mix - Human Inflammatory Chemokine 7-Plex Panel 1 Human Inflammatory Chemokine 7-Plex Panel 2 1 no Eotaxin, IL-8, RANTES, MCP-1, MCP-3, IP-10 1 x 96 wells BCA-1, MCP-1, MIP-1α, MIP-3β, PARC, PF4, RANTES 1 no Lyophilized premixed 7 Antigens (BCA-1, MCP-1, MIP-1α, MIP-3β, PARC, PF4, RANTES) 1 x 96 wells IP-10, I-TAC, MIP-1α, MIP-1β, 6Ckine, MDC, MIP-3β 18

20 Cat No HG19372 KC15514 HG19514 KC15516 KC15516T HG19516 Premixed Human Multiplex Kits Standard Mix - Human Inflammatory Chemokine 7-Plex Panel 2 Human CD8+ T Cell Related Cytokines, 14-plex Standard Mix Set - Human CD8+ T Cell Related Cytokines, 14-plex Human CD8+ T Cell Related Cytokines, 16-plex Human CD8+ T Cell Related Cytokines, 16-plex Standard Mix Set - Human CD8+ T Cell Related Cytokines, 16-plex KC14402 Human IGF I and IGF II 2-plex panel 1 no : IGF I (< 0.1% cross-reactivity with IGF II at 10,000pg/mL) : IGF II (< 1% cross-reactivity with IGF I at 10,000pg/mL) HG19402 Premixed Human IGF I and IGF II 2-plex 1 no lyophilized standard KB11206 TGFbeta1 1-Plex 1 x 96 wells UTGFB1AG Standard - TGFbeta1 1 no 1 no Lyophilized premixed 7 Antigens (IP-10, I-TAC, MIP-1α, MIP-1β, 6Ckine, MDC, MIP-3β) 1 x 96 wells GM-CSF, Granzyme B, IFNγ, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1α, MIP-1β, sfasl, 1 no Lyophilized premixed 14 Antigens (GM-CSF, Granzyme B, IFNγ, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1α, MIP-1β, sfasl, ) 1 x 96 wells GM-CSF, Granzyme B, IFNγ, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1α, MIP-1β, RANTES, scd137, sfasl, 1 x 32 wells GM-CSF, Granzyme B, IFNγ, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1α, MIP-1β, RANTES, scd137, sfasl, 1 no Lyophilized premixed 16 Antigens (GM-CSF, Granzyme B, IFNγ, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1α, MIP-1β, RANTES, scd137, sfasl, ) 19

21 Cat No Premixed Multiplex M-R-NHP KC21031 Mouse T Helper Cytokine 3-Plex Panel 1 1 x 96 wells IL-6, IL-10, MG28131 Standard Mix - Mouse T Helper Cytokine 3-Plex Panel 1 1 no Lyophilized premixed 3 Antigens (IL-6, IL-10, ) KC21032 Mouse T Helper Cytokine 3-Plex Panel 2 1 x 96 wells IFNγ, IL-10, MG2132 Standard Mix - Mouse T Helper Cytokine 3-Plex Panel 2 1 no Lyophilized premixed 3 Antigens (IFNγ, IL-10, ) KC21033 Mouse T Helper Cytokine 3-Plex Panel 3 1 x 96 wells IL-9, IL-10, MG28133 Standard Mix - Mouse T Helper Cytokine 3-Plex Panel 3 1 no Lyophilized premixed 3 Antigens (IL-9, IL-10, ) KC21041 Mouse T Helper Cytokine 4-Plex Panel 1 1 x 96 wells IFNγ, IL-4, IL-6, MG2141 Standard Mix - Mouse T Helper Cytokine 4-Plex Panel 1 1 no Lyophilized premixed 4 Antigens (IFNγ, IL-4, IL-6, ) KC21042 Mouse T Helper Cytokine 4-Plex Panel 2 1 x 96 wells IFNγ, IL-4, IL-10, MG28142 Standard Mix - Mouse T Helper Cytokine 4-Plex Panel 2 1 no Lyophilized premixed 4 Antigens (IFNγ, IL-4, IL-10, ) KC21043 Mouse T Helper Cytokine 4-Plex Panel 3 1 x 96 wells IL-6, IL-10, IL-22, MG28143 Standard Mix - Mouse T Helper Cytokine 4-Plex Panel 3 1 no Lyophilized premixed 4 Antigens (IL-6, IL-10, IL-22, ) KC20005 Mouse Th1/Th2 5-plex Panel 1 x 96 wells IFNγ, IL-2, IL-4, IL-5, KC20005T Mouse Th1/Th2 5-plex Panel 1 x 32 wells IFNγ, IL-2, IL-4, IL-5, MG28005 Standard Mix - Mouse Th1/Th2 5-plex 1 no Lyophilized premixed 5 Antigens (IFNγ, IL-2, IL-4, IL-5, ) KC21061 Mouse Th1/Th2 6-Plex Panel 1 1 x 96 wells IFNγ, IL-2, IL-4, IL-6, IL-10, MG28161 Standard Mix - Mouse Th1/Th2 6-Plex Panel 1 1 no Lyophilized premixed 6 Antigens (IFNγ, IL-2, IL-4, IL-6, IL-10, ) KC21062 Mouse Th1/Th2 6-Plex Panel 2 1 x 96 wells IFNγ, IL-2, IL-4, IL-5, IL-10, MG28162 Standard Mix - Mouse Th1/Th2 6-Plex Panel 2 1 no Lyophilized premixed 6 Antigens (IFNγ, IL-2, IL-4, IL-5, IL-10, ) KC20081 Mouse Th1/Th2 8-plex 1 x 96 wells IFNγ, IL-1α, IL-2, IL-4, IL-5, IL-6, IL- 10, and MG28181 Standard Mix - Mouse Th1/Th2 8-Plex 1 no Lyophilized premixed 8 Antigens (IFNγ, IL-1α, IL-2, IL-4, IL-5, IL-6, IL-10, and ) KC21107 Mouse Th1/Th2/Th17 7-plex Kit 1 x 96 wells IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, KC21107T Mouse Th1/Th2/Th17 7-plex Kit 1 x 32 wells IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, MG10007 Standard Mix - Mouse Group 1 Panel A, 7-Plex 1 no Lyophilized premixed 7 Antigens (IFNgamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFalpha) KC20010 Mouse Th1/Th2/Th17 10-plex 1 no GM-CSF, IFNgamma, IL-1alpha, IL- 2, IL-4, IL-5, IL-6, IL-10, IL-17A and TNFalpha KC20010T Mouse Th1/Th2/Th17 10-plex 1 x 32 wells GM-CSF, IFNgamma, IL-1alpha, IL- 2, IL-4, IL-5, IL-6, IL-10, IL-17A and TNFalpha MG28010 Standard Mix Set - Mouse TH1/Th2/Th17 10-Plex 1 no Lyophilized premixed 10 Antigens (GM-CSF, IFNgamma, IL-1alpha, IL-2, IL-4, IL-5, IL-6, IL-10, IL-17A and TNFalpha), 1 set of 2 vials KC21114 Mouse Th1/Th2/Th17 14-plex 1 x 96 wells IFNgamma, IL-1alpha, IL-1beta, IL- 2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22 and TNFalpha KC21114T Mouse Th1/Th2/Th17 14-plex 1 x 32 wells IFNgamma, IL-1alpha, IL-1beta, IL- 2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22 and TNFalpha 20

22 Cat No MG28114 Premixed Multiplex M-R-NHP Standard Mix Set - Mouse TH1/Th2/Th17 14-plex 1 no Lyophilized premixed 14 Antigens (IFNgamma, IL-1alpha, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL- 13, IL-17A, IL-21, IL-22 and TNFalpha), 1 set of 2 vials KC21116 Mouse Th1/Th2/Th17 16-plex 1 x 96 wells GM-CSF, IFNgamma, IL-1alpha, IL- 1beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL- 12p70, IL-13, IL-17A, IL-21, IL-23, KC, TNFalpha KC21116T Mouse Th1/Th2/Th17 16-plex 1 x 32 wells GM-CSF, IFNgamma, IL-1alpha, IL- 1beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL- 12p70, IL-13, IL-17A, IL-21, IL-23, KC, TNFalpha MG28116 Standard Mix Set - Mouse TH1/Th2/Th17 16-plex 1 no Lyophilized premixed 16 Antigens (GM-CSF, IFNgamma, IL-1alpha, IL- 1beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL- 12p70, IL-13, IL-17A, IL-21, IL-23, KC, TNFalpha), 1 set of 3 vials KC21118 Mouse Th1/Th2/Th17 18-plex 1 x 96 wells GM-CSF, IFNgamma, IL-1alpha, IL- 1beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL- 12p70, IL-13, IL-17A, IL-21, IL-22, IL- 23, KC, TNFalpha and TSLP KC21118T Mouse Th1/Th2/Th17 18-plex 1 x 32 wells GM-CSF, IFNgamma, IL-1alpha, IL- 1beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL- 12p70, IL-13, IL-17A, IL-21, IL-22, IL- 23, KC, TNFalpha and TSLP MG28118 Standard Mix Set - Mouse TH1/Th2/Th17 18-plex 1 no Lyophilized premixed 18 Antigens (GM-CSF, IFNgamma, IL-1alpha, IL- 1beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL- 12p70, IL-13, IL-17A, IL-21, IL-22, IL- 23, KC, TNFalpha and TSLP), 1 set of 3 vials KC22251 Mouse Inflammation 5-Plex Panel 1 1 x 96 wells IL-1β, IL-6, IL-10, MCP-1, MG28251 Standard Mix - Mouse Inflammation 5- Plex Panel 1 1 no Lyophilized premixed 5 Antigens (IL- 1β, IL-6, IL-10, MCP-1, ) KC22252 Mouse Inflammation 5-Plex Panel 2 1 x 96 wells IL-6, IL-10, IL-12p70, MCP-1, MG28252 Standard Mix - Mouse Inflammation 5- Plex Panel 2 1 no Lyophilized premixed 5 Antigens (IL- 6, IL-10, IL-12p70, MCP-1, ) KC22206 Mouse Inflammation 6-Plex Panel 1 x 96 wells IFNγ, IL-6, IL-10, IL-12p70, MCP-1, KC22206T Mouse Inflammation 6-Plex Panel 1 x 32 wells IFNγ, IL-6, IL-10, IL-12p70, MCP-1, MG19106 Standard Mix - Mouse Inflammation 6- Plex 1 no Lyophilized premixed 6 Antigens (IFNγ, IL-6, IL-10, IL-12p70, MCP-1, ) KC22271 Mouse Inflammation 7-Plex Panel 1 1 x 96 wells IFNγ, IL-1β, IL-6, IP-10, KC, MCP-1, MG28271 Standard Mix - Mouse Inflammation 7- Plex Panel 1 1 no Lyophilized premixed 7 Antigens (IFNγ, IL-1β, IL-6, IP-10, KC, MCP-1, ) KC22272 Mouse Inflammation 7-Plex Panel 2 1 x 96 wells IL-1α, IL-1β, IL-6, IL-10, IL-22, IL-23, MG28272 Standard Mix - Mouse Inflammation 7- Plex Panel 2 1 no Lyophilized premixed 7 Antigens (IL- 1α, IL-1β, IL-6, IL-10, IL-22, IL-23, ) KC22208 Mouse Inflammation 8-Plex Panel 1 x 96 wells IFNγ, IL-1α, IL-6, IL-10, IL-12p70, KC, MCP-1, KC22208T Mouse Inflammation 8-Plex Panel 1 x 32 wells IFNγ, IL-1α, IL-6, IL-10, IL-12p70, KC, MCP-1, 21

23 Cat No Premixed Multiplex M-R-NHP MG19108 Premixed mouse Inflammation 8-Plex lyophilized stnd 1 no IL-1α, IL-6, IL-10, KC, MCP-1, IFNgamma, TNFalpha, IL-12p70 KC22211 Mouse Inflammation 11-Plex 1 x 96 wells IFNgamma, IL-1alpha, IL-1beta, IL- 6, IL-10, IL-12p70, IL-23, IP-10, KC, MCP-1, TNFalpha KC22211T Mouse Inflammation 11-Plex 1 x 32 wells IFNgamma, IL-1alpha, IL-1beta, IL- 6, IL-10, IL-12p70, IL-23, IP-10, KC, MCP-1, TNFalpha MG28211 Standard Mix Set - Mouse Inflammation 11-Plex 1 no Lyophilized premixed 11 Antigens (IFNgamma, IL-1alpha, IL-1beta, IL-6, IL-10, IL-12p70, IL-23, IP-10, KC, MCP-1, TNFalpha), 1 set of 2 vials KC22217 Mouse Inflammation 17-Plex 1 x 96 wells IFNgamma, IL-1alpha, IL-1beta, IL- 6, IL-9, IL-10, IL-12p70, IL-13, IL- 15, IL-23, IP-10, KC, MCP-1, MIP- 1alpha, MIP-1beta, RANTES and TNFalpha KC22217T Mouse Inflammation 17-Plex 1 x 32 wells IFNgamma, IL-1alpha, IL-1beta, IL- 6, IL-9, IL-10, IL-12p70, IL-13, IL- 15, IL-23, IP-10, KC, MCP-1, MIP- 1alpha, MIP-1beta, RANTES and TNFalpha MG28217 Standard Mix Set - Mouse Inflammation 17-Plex 1 no Lyophilized premixed 17 Antigens (IFNgamma, IL-1alpha, IL-1beta, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL- 15, IL-23, IP-10, KC, MCP-1, MIP- 1alpha, MIP-1beta, RANTES and TNFalpha ), 1 set of 3 vials KC23308 Mouse Chemokine 7-Plex Panel 1 x 96 wells Eotaxin, KC, RANTES, MIG, MCP- 1, MCP-3, IP-10 KC23308T Mouse Chemokine 7-Plex Panel 1 x 32 wells Eotaxin, KC, RANTES, MIG, MCP- 1, MCP-3, IP-10 MG19307 Premixed Mouse Chemokine 7-Plex lyophilized stnd 1 no Eotaxin, KC, RANTES, MIG, MCP- 1, MCP-3, IP-10 KC Rat Th1/Th2/Th17 7-plex Kit 1 x 96 wells IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, KC31107T Rat Th1/Th2/Th17 7-plex Kit 1 x 32 wells IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, RG10007 Standard Mix - Rat Group 1 Panel A, 7- Plex 1 no Lyophilized premixed 7 Antigens (IFNgamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFalpha) KC41106 NHP Th1/Th2 6-plex Panel 1 x 96 wells IFNγ, IL-2, IL-4, IL-5, IL-6, KC41106T NHP Th1/Th2 6-plex Panel 1 x 32 wells IFNγ, IL-2, IL-4, IL-5, IL-6, NG10006 Standard Mix - NHP Th1/Th2 6-plex 1 no Lyophilized premixed 6 Antigens (IFNγ, IL-2, IL-4, IL-5, IL-6, ) KC41107 NHP Th1/Th2/Th17 7-plex Kit 1 x 96 wells IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, KC41107T NHP Th1/Th2/Th17 7-plex Kit 1 x 32 wells IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, NG10007 Standard Mix - NHP Group 1 Panel A, 7- Plex 1 no Lyophilized premixed 7 Antigens (IFNgamma, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFalpha) KC44402 NHP IGF I and IGF II 2-plex panel 1 x 96 wells NG46402 Premixed NHP IGF I and IGF II 2-plex 1 no lyophilized stnd KB11206 TGFbeta1 1-Plex 1 x 96 wells KB11206S Standard - TGFbeta1 1 no 22

24 Assay Specifications Sample types: Sensitivity (LOD): Quantitation range: LLOQ: ULOQ: Standard Dose Recovery: % Precision: Intra-assay CV: < 10% Inter-assay CV: < 20% Cross-reactivity of Analytes in a panel or group: Negligible Sample Volume: 15 µl Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate Majority of the assays < 10 pg/ml Majority of the assays < 20 pg/ml Majority of the assays > 5,000 pg/ml Representative Data Representative Standard Curves 23

25 Correlation with Luminex* Assays Cell culture supernatant samples from human PBMCs treated with various reagents were assayed by using KrishPlex human Th1/2/17 7-Plex assays and a commercially available Luminex multiplex assay kit. KRISHPLEX Assays were analysed on a BD FACS Calibur* *Trademarks are the property of their respective owners Correlation with CBA* Assays Human normal and abnormal serum samples were measured with both KrishPlex and CBA assays and analysed on a BD FACSCanto II. *Trademarks are the property of their respective owners 24

26 Advantages of KRISHPLEX over BD s Cytometric Bead Arrays (CBA) A more comprehensive menu Competitive pricing Expedited Custom Panel development More user-friendly assay kits based on microplate format, not BD s FACS Tubes Custom premixed panels at NO EXTRA CHARGE 3-fold Standard curve dilution increases low-end detection More flexible assay panels Quantitative Bead-Based Assays In The Market: Magnetic Bead Based Plate Technology Non Magnetic Bead Based FC Technology R&D Systems Bio- Plex* xmap* Procarta plex* Milliplex* KrishPlex CBA LegendPlex Many Flow Cytometers Luminex Platform only * Trademarks are the property of their respective owners 25

27 FAQs Which sample types are suitable for Krishplex Immunoassays? Human, non-human primate, mouse and rat cell culture supernatant, serum, plasma, cell/tissue lysate and other body fluid such as saliva, BALF, pleural fluid, peritoneal fluid, synovial fluid and cerebrospinal fluid. What is the maximum multiplex level? Up to 24-plex. What flow cytometer can be used? Any flow cytometry analyzer equipped with a 488nm laser. Does it need a plate loader? No. Process the assay on a filter plate. Transfer to sample tube and run on a flow cytometer without a plate loader. Can I use a Luminex* to analyze Krishplex assays? No. Any other equipment needed? Do NOT invert the plate to dump the liquid during the assay procedure. optional, you may purchase the following: i) Krishplex Ezprep Filter Plate Washer (PN: VM1001) and; ii) Plate shaker. The beads do not adhere to the plate. alternately, After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette. Can I create my own multiplex panel? Yes, by ordering multiple Plex-One kits within a group. Custom premixed panels are also available for 6 or more analytes in a panel without extra charge (please allow 10 business days for delivery). If the assay/panel I want is not available, what do I do? We offer custom premixed multiplex panels as well as custom assay development for analytes not currently available. We will work with you to design the panels and assays. Please contact us for pricing, lead time and details of those offerings. How do I analyze the data? We offer with every kit shipped a free of charge software. You may also on purchase, us for a link to download the software. 26