Use of sheep horn hydrolysate as nitrogen source for lactic acid bacteria growth

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Sydney Wiggins
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1 Use of sheep horn hydrolysate as nitrogen source for lactic acid bacteria growth Ayat A A, Zaid A T, Hayder H I Biotechnology Research Center/ AlNahrain University ( SHH ) Sheep Horn Hydrolysate ( SHHM ) ( ) Lactobacillus acidophilus nm 540 Lactobacillus SHHM ( ) acidophillus Klebsiella sp ( 18, 13, 14 ) SHHM Candida sp Staphylococcus aureus Klebsiella sp ( 12,10,12 ) Candida sp Staphylococcus aureus Klebsiella sp ( 20, 14, 14 ) SHHM ( 16, 13, 13 ) Candida sp Staphylococcus aureus Candida sp Staphylococcus aureus Klebsiella sp / Abstract This study included used the sheep horn Hydrolysate as a nitrogen source in growth medium (SHHM) for cultivation of lactic acid bacteria, The results showed the optical density of growth in 540 nm for and lactobacillus acidophillus was 138 and 131 respectively after cultivation in (SHHM) medium Otherwise the optical density of growth for and lactobacillus acidophillus was 127 and 132, respectively after cultivation in () medium, Which contain peptone, yeast extract and meat extract as a nitrogen source In the inhibition capability test the results showed that the inhibition zone average of cell free extract for lactobacillus casei which cultivated in SHHM was (14, 13 and 18) mm against Klebsiella sp, Staphylococcus aureus and Candida sp respectively, while the inhibition zone average of cell free extract for lactobacillus casei which cultivated in was (12, 10 and 12 mm) against Klebsiella sp, Staphylococcus aureus and Candida sp Respectively The 79

2 inhibition zone average of cell free extract for lactobacillus acidophillus which cultivated in SHHM was (14, 14 and 20) mm against Klebsiella sp, Staphylococcus aureus and Candida sp respectively, and ( 13, 13 and 16 ) mm against Klebsiella sp, Staphylococcus aureus and Candida sp respectively after cultivated in medium 1 ],Glutamine ) Lactobacillus ( Fastidious ) Lactobacillus Lactobacillus [3] [ 2 ( Arginine,Leucine,Isoleucine, Valine, Phenyl alanine, Tryptophane Lactobacillus helveticus Lactobacillus [4] Lactobacillus plantarum acidophillus B12 [5] [6] [7] ( Prebiotic ) ) ( 6HCL No1 Sheep Horn [8] 4 Agar 15 % ) ( Candida sp Staphylococcus aureus Klebsiella sp? Horn Flour (HF) ( / ) % 4 Hydrolysate (SHH) 1 (Himedea) Mueller Hinton 2 (Difico) 80

3 3 () Sodium acetate trihydrate 5 ( / ) % 4 MgSO47H2O 02 Triammonum citrate 2 3 Tween80 1 Dglucose 20 4 (SHHM2) MnSO47H2O 005 Dglucose 20 ( / ) % 5 ( / ) % 4 (SHHM3) 02 Triammonum citrate 2 Tween80 1 Sodium acetate trihydrate MnSO47H2O 005 MgSO47H2O 15 2 ( / 15 ) 121 [9] Cell Free Extract (CFE) 1 37 % / 5000 µ m 045 [9] Well Assay LShape 37 5 (1) CFE [10] 540nm (2 1 ) 127 SHHM3 SHHM2 ( ) ( ) ( 132 ) %14, ( SHHM3 SHHM2 ) [8] % 22 [ 2 1 ] [11] Cysteine SHHM3 Keratin SHHM2 81

4 540nm 24 ( 1 ) nm ( 2 ) ( 1 ) Klebsiella sp ( ) Candida sp Staphylococcus aureus Candida sp Staphylococcus aureus Klebsiella sp ( ) ( 2 ) Klebsiella sp ( ) Candida sp Staphylococcus aureus Candida sp Staphylococcus aureus Klebsiella sp ( ) Prebiotic ( ) Probiotic 82

5 ( 1 ) Candida sp Staphylococcus aureus Klebsiella sp ( 2 ) Candida sp Staphylococcus aureus Klebsiella sp Holt, jc; Krieg, NR; Sneath, PH; Staley, JT and Williams, TS (1994) Bergey's manual of determinative bacteriology, 9 th Ed Williams and Wilkins Company Baltimore Maryland, USA 2 Teuber, M (1995) The Genus LactococucIn The Genera of Lactic Acid Bacteria Edited by Wood, BJ and Holzapfel, WH 3 Morishita, T; Kegushi, Y; Yajima, M; Sakurai, T and Yura, T (1981) Multiple nutritional requirements of Lactobacilli genetic lesion affecting amino acid biosynthetic pathways J Bact, ( cited from Neto and Yokoya, 1997) 4 Narendranath, NV; Hynes, SH; Thomas, KC and Ingledew, WM (1997) Effect of Lactobacilli and yeast Catalyzed ethanol fermentations J Appl Environ Microbiol, 63(11) De la Broise, D, Dauer, G, Gildberg, A, Guerard, F(1998) Evidence of positive effect of peptone hydrolysis rate on Escherichia coli culture kinetics J Mar Biotechnol Dufosse,L,DeLaBroisse,D,Guerard,F(1997)Fiproteinhydrolysates as nitrogen sources for microbial growthandmetabolite production Recent Res Devel In Microbiol KurbanoUlu, EB(2001) Production of singlecell protein from ram horn hydrolysate Turk J Biol Esabi, BK And Omer, FA(2002) Use of Ram Horn Hydrolysate as Peptone for Bacterial Growth Turk J Biol Gupta, PK ; Mital, BK and Garg, SK ( 1996) Characterization of Lactobacillus acidophillus strain for use as dietary adjunct Int J of Food Microbiology Harrigan, W F and McCance, M E (1976) Laboratory methods in microbiology Academic Press London UK 83

6 11 Dalev, P(1990) An enzymealkaline hydrolysis of feather keratin for obtaining a protein concentrate for fodder Biotechnol Lett Vanderola, C G; Bailo, N and Reinheimer, J A (2000) Survival of Probiotic microflora in yoghurts during refrigerated storage J Food Research International, Vol Casas, IA and Dobrogosz, WJ (2000) Validation of the Probiotic concept Lactobacillus reuteri confers broadspectrum protection against disease in humans and animals Microbial Ecol Health Dis

PROBIOTIC BACTERIA AS YOGHURT STARTER AND ITS IMPLICATION EFFECT TO THE PATHOGENIC AND NON PATHOGENIC BACTERIA IN MICE GASTROINTESTINAL

PROBIOTIC BACTERIA AS YOGHURT STARTER AND ITS IMPLICATION EFFECT TO THE PATHOGENIC AND NON PATHOGENIC BACTERIA IN MICE GASTROINTESTINAL Lovita Adriani, Hendronoto A.W. Lengkey Faculty of Animal Husbandry,