Biogents Sweetscent Lure Increases the Collection Rate of Aedes aegypti and Aedes albopictus in Commercially Available Mosquito Traps
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1 Biogents Sweetscent Lure Increases the Collection Rate of Aedes aegypti and Aedes albopictus in Commercially Available Mosquito Traps Ingeborg Schleip, Daniel Kline, Joyce Urban, Scott Willis, Charles Abadam, Jay Kiser, Karen Akaratovic, Scott Gordon, Martin Geier Joint International Tropical Medicine Meeting 2017
2 The mosquito attractant BG-Sweetscent The BG-Sweetscent developed to enhance the catches of mosquito traps for Asian tiger mosquitoes (Aedes albopictus) and yellow fever or dengue mosquitoes (Aedes aegypti). Both species are important vectors of diseases such as dengue and chikungunya imitates human scent with three components found on the skin lasts up to 2 months EPA registration in the USA since 2016 (EPA Reg. No.: ) 2
3 The mosquito attractant BG-Sweetscent The BG-Sweetscent works very well with Biogents mosquito traps. Question: Will the BG-Sweetscent also increase the catch rates for Aedes aegypti and Aedes albopictus with other commercially available mosquito traps? 3
4 Seven tested mosquito traps in the USA Test of six commercially available traps for homeowner use and one researcher trap: Price ranging from USD Bought from local home improvement stores online merchants Dynatrap DT2000XL / Dynatrap DT V Bite Shield MK06 Protector Blue Rhino Bite-Guard SVE6211 MosClean UV LED Galaxie Flowtron PV75 Black Flag Zapper CDC Miniature Light Trap USD / USD USD USD USD USD USD Suction trap Suction trap Suction trap Suction trap Suction trap Zapper trap Electrocution Suction trap 4
5 Field studies with traps for homeowner use Two studies from independent organisations tested different traps with similar study design: Gainesville, Florida (USDA (United States Department of Agriculture)): Aedes aegypti and Aedes albopictus 5 different traps, 20 trap nights Lake Charles, Louisiana (Calcasieu Mosquito Control): Aedes albopictus 3 different traps, 36 trap nights Each trap was tested with and without the BG-Sweetscent attractant Modified Latin square and a Latin square test Switching trap position/location No CO 2 was added to the traps 5
6 Results: Gainesville, Florida Dynatrap DT2000XL Bite Shield MK06 Protector Blue Rhino Bite-Guard SVE6211 MosClean UV LED Galaxie Flowtron PV75 Aedes aegypti Suction trap Suction trap Suction trap Suction trap Suction trap Increase in catch rates with BG-Sweetscent 6x 10x 1.2x 10x Total number collected Aedes albopictus Increase in catch rates with BG-Sweetscent 2x 9x 2x 4.5x Total number collected
7 Results: Gainesville, Florida For all trap types except the Bite Guard, catch rates for all mosquitoes could be improved. 7
8 Results from Lake Charles, Louisiana Dynatrap DT V Blue Rhino Bite-Guard SVE6211 Black Flag Zapper Aedes albopictus Suction trap Suction trap Zapper trap Increase in catch rates with BG-Sweetscent 2x 3x 4x Total number collected
9 Results from Lake Charles, Louisiana For two trap types, catch rates for all mosquitoes could be improved, for the Black Flag (zapper trap) it decreased. 9
10 Field study with a research trap: CDC light trap A study from an independent organisation tested the widely used research trap CDC miniature light trap : Suffolk, Virginia (Suffolk Mosquito Control): Aedes albopictus, 4 CDC traps, 8 trap nights Traps were tested without attractant, with BG-Sweetscent, with CO 2, and with BG-Sweetscent and CO 2 With incandescent light Latin square test Switching trap position/location 10
11 Results from Suffolk, Virginia Aedes albopictus x 3 4x fold increase for CDC light traps without CO 2 when run additionally with BG-Sweetscent 8.5 fold increase for CDC light traps with CO 2 when run additionally with BG-Sweetscent Great example for the synergistic effect of CO 2 and BG-Sweetscent 11
12 Summary Homeowner traps Study in Florida: times more captured Aedes albopictus times more captured Aedes aegypti Study in Louisiana: 2-4 times more captured Aedes albopictus CDC miniature light trap Study in Virginia: BG-Sweetscent and CO 2 are synergists 8.5 times more captured Aedes albopictus than just with CO 2 12
13 Summary BG-Sweetscent can greatly increase the catching success for Ae. aegypti and Ae. albopictus when using mosquito traps for homeowner use For mosquito control programs using CO 2 baited CDC light traps, the addition of BG-Sweetscent can dramatically increase collections of Ae. albopictus Additionally, in most traps tested, adding BG-Sweetscent also increased overall mosquito collections BG-Sweetscent is the first attractant for Ae. aegypti and Ae. albopictus that is scientifically proven to work 13
14 Acknowledgements Thank you to the authors of the studies: Daniel Kline and Joyce Urban from the CMAVE of the USDA (United States Department of Agriculture) Scott Willis from the Calcasieu Mosquito Control Charles Abadam, Jay Kiser, and Karen Akaratovic from the Suffolk Mosquito Control Scott Gordon and Martin Geier from Biogents AG Thank you for your attention! The presentation is available at www. biogents.com/pdf/ccc.pdf 14
15 Appendix
16 Study design in Gainesville, Florida A Daily switch over 4 nights Daily switch over 4 nights B weekly E weekly 2x (2x2) Latin square at 5 positions: 4 nights at 5 positions: 20 trap nights 18:00-8:00 weekly C Daily switch over 4 nights D Daily switch over 4 nights Daily switch over 4 nights
17 Study design in Lake Charles, Louisiana: Latin square B A C F daily 6x (6x6) Latin square: Each trap 6 x at each of the 6 locations: 36 trap nights 18:00-8:00 daily D E
18 Study design in Suffolk, Virginia A B daily daily 2x (4x4) Latin square: Each trap 2 x at each location: 8 trap nights 18:00-8:00 daily D C
19 Tested mosquito traps Dynatrap DT2000XL / Dynatrap DT V Bite Shield MK06 Protector Blue Rhino Bite-Guard SVE6211 MosClean UV LED Galaxie Flowtron PV75 Black Flag Zapper CDC Miniature Light Trap UV bulbs + TiO2, fan, warmth UV bulbs + TiO2, fan, warmth UV bulbs + TiO2, fan, warmth UV LEDs + TiO2, fan UV bulbs + fan, warmth UV bulbs + warmth Incandescent light, fan
20 Collected mosquito species Study in Louisiana: Psorophora ferox Ps. ciliata Ps. columbiae Mansonia titillans Culex quinquefasciatus Cx. salinarius Cx. nigripalpus Cx. erraticus Cx. coronator Aedes albopictus Ae. aegypti Ae. atlanticus Ae. fulvus pallens Ae. sollicitans Ae. taeniorhynchus Ae. vexans Anopheles quadrimaculatus An. crucians Coquillettidia perturbans Study in Florida: Aedes albopictus Ae. aegypti Ae. atlanticus Ae. infirmatus Mansonia perturbans Culex erraticus Uranotaenia spp Anopheles crucians
21 US EPA Approval We received US EPA approval for our BG-Sweetscent. The EPA allowed us to use following claims: Mosquito attractant Developed for hard-to-catch species of mosquitoes and other biting insects, including those which may transmit Zika virus, Chikungunya or Dengue Effective in attracting a broad range of mosquitoes species, including those which may transmit diseases such as West Nile virus, Encephalitis, Zika virus, Chikungunya or Dengue Designed for use with any mosquito trap Attracts Aedes albopictus, Asian Tiger Mosquito Increase your catch rates Mimics naturally occurring human skin scents which attract mosquitoes
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