Improvement of the liquid-chromatographic analysis of protein tryptic digests by the use of long-capillary monolithic columns with UV and MS detection
|
|
- Cori Washington
- 5 years ago
- Views:
Transcription
1 Anal Bioanal Chem (2007) 388: DOI /s ORIGINAL PAPER Improvement of the liquid-chromatographic analysis of protein tryptic digests by the use of long-capillary monolithic columns with UV and MS detection M. H. M. van de Meent & G. J. de Jong Received: 10 November 2006 / Revised: 29 January 2007 / Accepted: 16 February 2007 / Published online: 13 March 2007 # Springer-Verlag 2007 Abstract Optimisation of peak capacity is an important strategy in gradient liquid chromatography (LC). This can be achieved by using either long columns or columns packed with small particles. Monolithic columns allow the use of long columns at relatively low back-pressure. The gain in peak capacity using long columns was evaluated by the separation of a tryptic bovine serum albumin digest with an LC UV mass spectrometry (MS) system and monolithic columns of different length (150 and 750 mm). Peak capacities were determined from UV chromatograms and MS/MS data were used for Mascot database searching. Analyses with a similar gradient slope for the two columns produced ratios of the peak capacities that were close to the expected value of the square root of the column length ratio. Peak capacities of the short column were 12.6 and 25.0 with 3 and 15 min gradients, respectively, and 29.7 and 41.0 for the long column with 15 and 75 min gradients, respectively. Protein identification scores were also higher for the long column, 641 and 750 for the 3- and 15-min gradients with the short column and 1,376 and 993 for the 15- and 75-min gradients with the long column. Thus, the use of long monolithic columns provides improved peptide separation and increased reliability of protein identification. Keywords Peak capacity. Monoliths. Long columns. Gradient liquid chromatography. Protein identification M. H. M. van de Meent (*) : G. J. de Jong Division of Biomedical Analysis, Department of Pharmaceutical Sciences, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands m.h.m.vandemeent@pharm.uu.nl Introduction Identification of unknown proteins is a key step in proteome analysis. The standard method of protein identification consists of enzymatic digestion of the protein(s), usually by using trypsin, followed by mass spectrometry (MS) analysis of the resulting digest. When analysing a single protein, e.g. from an excised 2D gel spot, a peptide separation is usually unnecessary. When analysing a digest of a mixture of proteins, the resolution of only a mass spectrometer is usually not sufficient. In those cases, a peptide separation is applied before MS detection. In shotgun proteomics, where a whole proteome is digested without prior protein fractionation, 2D liquid chromatography (LC) is the method of choice owing to the high complexity of the peptide mixtures. A drawback of multidimensional methods is the time frame, as typical analysis times for 2D-LC analysis of peptide mixtures range from several hours to more than 1 day [1 4]. 1D-LC methods can be used for separation of less complex samples (digests of fewer than 100 proteins), but this requires very efficient separations in combination with the additional separation power of MS detection. Such highefficiency separations have been reported for analyses with long, packed columns, using (ultra-) high pressure (about 70 MPa) systems [5, 6]. Peak capacity is the primary parameter for evaluation of efficiency in gradient chromatography [7]. Peak capacity was first defined by Giddings [8] as the maximum number of peaks that can be separated by a phase system. This theory was later adapted for gradient chromatography by Horváth and Lipsky [9]. There are generally two approaches for optimisation of the peak capacity. The first is increase of the gradient length; gradients of up to 10 h have been reported on single columns [10]. However,
2 196 Anal Bioanal Chem (2007) 388: according to theory, peak capacity increases to a maximum and then decreases as the gradients become longer [11, 12]. The second approach is the use of longer columns, as the peak capacity increases linearly with the square root of the plate number and thus with the square root of the column length. Wang et al. [13] illustrated this by connecting several columns packed with a pellicular stationary phase. They showed that the ratio of the peak capacity and the square root of the column length was constant for columns ranging in length from 7.5 to 60 cm. The main limitation to simply increasing column length is the higher backpressure. A possible solution for this problem is the use of monolithic columns. Monolithic columns have a higher permeability compared with packed columns, facilitating fast separations or the use of long columns [10, 14 16]. Wang et al. [13] reported a back-pressure of 28.5 MPa for a 60 cm 2.1 mm packed column at a linear flow of 0.94 mm/ s. In contrast, other groups have obtained 3 times higher linear flow rates for monolithic columns of similar length [10, 17]. The higher permeability of monolithic columns makes it possible to operate long columns at higher flow rates while using conventional LC equipment. Luo et al. [10] separated a bacterial protein digest at a linear flow rate of 2.4 mm/s with a 70-cm monolithic column at 34.5 MPa (5,000 psi). Tolstikov et al. [17] analysed plant metabolomic extracts with a flow rate of 2.6 mm/s for a 60-cm column. The quality of the analysis of a protein digest can be expressed in different ways. One way is to describe chromatographic efficiency in terms of parameters like peak width, peak capacity and resolution. Another way is to use the reliability of protein identification, like SEQUEST [18] or Mascot ( [19] scores. Such an approach is also useful, as the mass spectrometer adds a second dimension to the separation, which is overlooked when only chromatographic efficiency is measured. In this paper the evaluation of capillary monolithic silica columns of different lengths for the LC-UV-MS analysis of a bovine serum albumin (BSA) tryptic digest is described. Columns of 150- and 750-mm length were investigated using gradient times varying from 3 to 75 min. Chromatographic peak capacities, based on UV detection, and protein identification, based on Mascot scoring data of the MS detection, were determined as a measure for the efficiency of peptide separation. Theoretical aspects The peak capacity is defined as the maximum number of bands that will fit within a chromatogram with a resolution of R s =1.0 [20]. In gradient LC, where peak width is about constant throughout the separation, the theoretical peak capacity is given by Eq. 1: PC ¼ 1 þ t g ; ð1þ w av where t g is the gradient time and w av is the average baseline peak width. For large values of PC, this approaches t g /w av. Because sample peaks often do not occupy the entire length of the gradient, the sample peak capacity can then be defined as PC ð ¼ t z t a Þ w av ¼ Δt w av ; ð2þ where t a and t z are the retention times of the first and last eluting peaks, respectively. Wang et al. [13] investigated the effect of column length on peak capacity in packed columns pffiffiffi and found that the peak capacity is proportional to L if the gradient slope is proportional to L. The gradient slope can be defined as Δφt 0 ; ð3þ t g where Δφ is the change in organic modifier fraction during the gradient (0 φ 1) and t 0 is the column dead time. If the linear flow rate is constant, t 0 is only dependent on column length; therefore, t g should be varied proportionally to column length in order to keep the gradient slope constant. Experimental Materials and reagents BSA, trypsin (porcine, type IX-S, EC ) and 1,4- dithiothreitol (DTT) were purchased from Sigma (St. Louis, MO, USA), and iodoacetamide (IAA) and NH 4 HCO 3 from Fluka (Buchs, Switzerland). Acetonitrile was high-performance LC gradient grade (Biosolve, Valkenswaard, the Netherlands), and spectroscopy-grade trifluoroacetic acid (TFA) was obtained from Merck (Darmstadt, Germany). All solutions were prepared using water from a Milli-Q water-purifying system (Millipore, Bedford, MA, USA). All reagents for the digestion of BSA were prepared in 200 mm NH 4 HCO 3 buffer, ph 8. The tryptic digest was prepared as follows. A 100-μl aliquot of BSA stock solution (3.5 μg/μl in water) was set to ph 8 by adding 25 μl of1mnh 4 HCO 3 buffer (ph 8). After addition of 25 μl of 10 mm DTT solution, the sample was incubated at 50 C for 30 min to reduce disulfide bonds. After cooling to room temperature, 25 μl of 30 mm IAA solution was added and the sample was incubated in the dark for 60 min to alkylate the free thiols. Trypsin was dissolved in 10 μl of buffer to reach a trypsin-to-protein mass ratio of 1:50 in the final solution; the trypsin solution was added to the sample,
3 Anal Bioanal Chem (2007) 388: which was incubated at 37 C overnight for 15 h. The digestion was stopped by addition of 15 μl of 10% TFA. The sample was diluted to 200 ng/μl (3 μm) with LC mobile phase A (water plus 0.05% TFA) and injected without further purification. Apparatus and LC columns All analyses were performed with an Agilent 1100 nanolc system (Agilent Technologies, Waldbronn, Germany), consisting of a vacuum degasser, a binary Nano-Pump, a μ- well plate sampler and a column switching module with a trapping column in the 1 4 position of the six-port columnswitching valve. The trapping pump was a Gynkotek model 480 (Gynkotek, Germering, Germany). Detection was performed by UV and MS detection, with the detectors connected in series. The UV detector was an MU 701 UV VIS detector (ATAS GL International, Veldhoven, the Netherlands), equipped with an external optical-fibre flow cell (6 nl, 3-mm light path); peptides were detected at 215 nm. The mass spectrometer was an Agilent LC/MSD Trap XCT (Agilent Technologies, Waldbronn, Germany) ion-trap mass spectrometer, equipped with an orthogonal electrospray ionisation (ESI) interface. The external flow cell of the UV detector allows minimal time delay and band-broadening between UV and MS detection. The monolithic columns were provided by GL Sciences (Tokyo, Japan). The columns were a 150 mm 0.1 mm MonoCap for nano-flow C18-silica monolithic column and a 750 mm 0.2 mm MonoCap high resolution C18-silica monolithic column. For trapping of the digest a 5 mm 0.3 mm column packed with 5 μm Zorbax 300 SB-C18 (Agilent Technologies, Waldbronn, Germany) was used. Method and data analysis LC solvent A was water plus 0.05% TFA (v/v); solvent B was acetonitrile plus 0.04% TFA (v/v). The trapping a 75 b c 35 d Fig. 1 Liquid chromatography (LC) UV chromatograms of a tryptic bovine serum albumin (BSA) digest, separated by monolithic silica capillary columns of 150 mm 0.1 mm (a, c) and 750 mm 0.2 mm (b, d) using a gradient of 5 50% acetonitrile (0.04% v/v trifluoroacetic acid, TFA) in water (0.05% v/v TFA). a A 3-min gradient (15%/min); b, c 15-min gradient (3%/min); d 75-min gradient (0.6%/min). The run times include 5 min of trapping and 2.5 and 12.5 min of gradient delay time for the 150-mm and 750-mm columns, respectively
4 198 Anal Bioanal Chem (2007) 388: Table 1 Chromatographic parameters from liquid chromatography UV analysis 150 mm 0.1 mm 750 mm 0.2 mm t g (min) w av Δt PC** w av Δt PC t g gradient time, w av average width of seven to 12 peaks which were found to contain only a single peptide (based on mass spectrometry data), Δt elution window between the first and last eluting peptides, PC** sample peak capacity (Δt/w av ) solvent was a mixture of 5% (v/v) solvent B in solvent A. After injection of the digest (0.25 μl for the 0.1-mm inner diameter column and 1.0 μl for the 0.2-mm inner diameter column), the sample was trapped on the trapping column at a flow rate of 5 μl/min. After 5 min, the trapping column was switched on-line with the separation column and the gradient was started. All separations were performed at room temperature using a gradient of 5 50% solvent B with gradient times varying from 3 to 75 min. MS spectra were acquired in the positive ion mode over the 400 2,000 m/z range, after which the two most intense ions (with a preference for doubly charged ions) were selected for fragmentation. MS/MS fragmentation spectra were acquired over the m/z range. An ESI spray voltage of - 3 kv was used for all experiments. The effect of separation efficiency on protein identification was evaluated using the Mascot search engine [19]. LC-MS/MS data were converted to the Mascot generic format (.mgf file) using the data-analysis software, and the.mgf files were searched against the MSDB database using Mascot s MS/MS ion search module. The database was searched for tryptic peptides from all entries in the database, allowing one missed cleavage per peptide and containing carbamidomethyl cysteine as a variable modification. Mass tolerances were set to default values: peptide mass tolerance ±2.0 Da, MS/MS tolerance ±0.8 Da. Results and discussion Liquid chromatography UV analysis Because of the difference in diameter, the 150 mm 0.1 mm and the 750 mm 0.2 mm columns were used with different flow rates. For the 150- and the 750-mm columns, the flow rates were 0.5 and 2.0 μl/min, respectively, resulting in a linear flow rate of 1.06 mm/s. Injection volumes were also proportional to the square of the column diameter, 0.25 μl of the digest for the 0.1-mm column and 1.0 μl onto the 0.2-mm column. During the gradient, the maximum backpressure of the 750-mm column was below 20 Mpa, which is well below the manufacturers limit of 30 Mpa. Figure 1 shows the LC-UV chromatograms of 3- and 15- min gradients run on the 150-mm column and 15- and 75- min gradients run on the 750-mm column. When the chromatograms of the analyses with similar gradient slopes are compared (Fig. 1a,b, and Fig. 1c,d), it is clear that an increase in column length improves the peptide separation. In order to quantify the efficiency of the separation, the sample peak capacity was calculated for all analyses. Because of the incomplete resolution of the digest, the peak capacity was estimated by using the average peak width of a selected number of peaks that appeared to contain only a single peptide. Using this method, we calculated peak capacities for all analyses and the results are summarised in Table 1. The peak capacities found for the short column are comparable to those found in the literature for similar columns [21, 22]. As expected, the peak capacities of the long column are higher than those of the short column, but they are relatively low compared with the values reported in [10]. However, when gradient time is taken into consideration, the difference is significantly less: PC**/t g is 0.55 peaks per minute with the present system (75-min gradient on the 750-mm column) and 1.62 peaks per minute for the 260-min gradient reported in [10]. A possible negative effect on the resolution of our system is the use of a trapping column, filled with a different Table 2 Mascot database search results 150 mm 0.1 mm 750 mm 0.2 mm t g Mascot score Peptides a Coverage b (%) Mascot score Peptides a Coverage b (%) Infusion c min min , min a Number of unique peptides identified by database search. b Percentage of amino acid sequence covered by identified peptides. c Direct infusion of the digest into the mass spectrometer.
5 Anal Bioanal Chem (2007) 388: Intens 6. x10 3 a Intens. x b 2 * * Time [min] Fig. 2 LC mass spectrometry (MS) base peak chromatograms and mass spectra of tryptic BSA digest, separated by monolithic silica columns of 150 mm 0.1 mm (a) and 750 mm 0.2 mm (b), using a gradient of 5 50% acetonitrile (0.04% v/v TFA) in water (0.05% v/v Time [min] TFA). a A 15-min gradient (3%/min); b 75-min gradient (0.6%/min). The run times include 5 min of trapping and 2.5 and 12.5 min of gradient delay time for the 150-mm and 750-mm columns, respectively stationary phase, which probably has a selectivity that differs from that of the analysis column. If chromatograms with the same gradient slope are compared (3- and 15-min gradients for the 150-mm column and 15- and 75-min gradients for the 750-mm column, respectively), the ratio of the peak capacities should be close to the square root of the column length ratio (2.24). For the 3-/15-min gradient pair this ratio is 29.7/12.6=2.37; for the 15-/75-min gradient pair the ratio is 41.0/25.0=1.64. For the short column, no increase in peak capacity is observed with a gradient of more than 15 min, but for the long column the peak capacity increases up to a 75-min gradient. A possible explanation can be found in the study of Stadalius et al. [11, 12], who demonstrated that peak capacity will increase with gradient time, until it reaches a maximum, after which it will even decrease. The gradient time at which this maximum is obtained is greater for longer columns. Liquid chromatography mass spectrometry analysis To assess the influence of peptide separation on protein identification, MS/MS data were investigated by database- Fig. 3 Averaged mass spectra of peptide YICDNQDTISSK (m/z , M2H 2+ ) as identified from extracted ion chromatograms in the LC-MS analysis of a tryptic BSA digest. a A 150-mm 0.1-mm silica monolithic column, 15-min gradient of 5 50% acetonitrile (0.04% v/v TFA) in water (0.05% v/v TFA); b 750-mm 0.2-mm column, 75-min gradient
6 200 Anal Bioanal Chem (2007) 388: searching using the MS/MS ion search module from the Mascot search engine. The results are expressed as a Mascot score, the number of unique identified peptides and the percentage of the BSA amino acid sequence covered by these peptides (Table 2). These results were compared with the scores obtained for direct infusion of the BSA digest at the same flow rates as for the LC separations. The protein identification parameters for the infusion experiments were about similar for both flow rates. All database searches gave bovine albumin as the top protein match and the only other significant matches were albumins from other species. Figure 2 shows base peak chromatograms of separations with the same gradient slope, a 15-min gradient for the 150- mm column and a 75-min gradient for the 750-mm column. The Mascot scores for the 750-mm column are higher than those for the 150-mm column. The average score per identified peptide is between 52 and 60 for the long column and between 42 and 51 for the short column. Combined with the larger number of identified peptides on the long column, this adds up to a higher Mascot score (Table 2). Despite providing only a low resolution, even the shortest gradients cause a considerable increase in the reliability of protein identification. Increase in gradient length leads to cleaner mass spectra (Fig. 3) and a higher number of identified peptides and sequence coverage, compared with direct infusion. These numbers, however, decrease beyond a certain gradient time. This could possibly be attributed to the lower peak heights for longer gradient times. This result indicates that there is a gradient slope where an increase in chromatographic separation no longer improves protein identification. Conclusions The use of long silica-based capillary monolithic columns provides a clear advantage over use of shorter columns, i.e. an increase of chromatographic efficiency and reliability of protein identification. As expected from chromatography theory, a factor 5 longer column gives a times increase in peak capacity for separations with similar gradient slope. The use of longer gradients also leads to an initial improvement of the protein identification score, but the score seems to have a maximum at longer gradient times. While the use of longer columns for the separation of peptides has a clear advantage because of the gain in chromatographic efficiency, this also gives a longer analysis time. As maximum protein identification scores for rather simple digests are reached at relatively short gradient times, it is important to find a compromise between chromatographic efficiency and analysis time. However, if the sample is more complex, the use of longer columns is more attractive as longer gradients are necessary to achieve sufficient separation. In the near future, short and long columns of the same diameter (0.1-mm inner diameter) will be compared. Further improvement of the separation might be obtained by optimisation of the combination of the trap column and the analysis column. Moreover, the potential of longer monolithic capillary columns will be demonstrated by the analysis of more complex and real samples. Acknowledgement The authors would like to thank M. Kurano of ATAS GL International for generously providing the monolithic capillary columns. This work was supported by The Netherlands Proteomics Center ( References 1. Delahunty C, Yates JR III (2005) Methods 35: Dai J, Shieh CH, Sheng Q, Zhou H, Zeng R (2005) Anal Chem 77: Washburn MP, Wolters D, Yates JR III (2001) Nat Biotechnol 19: Opiteck GJ, Jorgenson JW, Anderegg RJ (1997) Anal Chem 69: Shen Y, Smith RD (2002) Electrophoresis 23: Shen Y, Zhao R, Belov ME, Conrads TP, Anderson GA, Tang K, Pasa-Tolic L, Veenstra TD, Lipton MS, Udseth HR, Smith RD (2001) Anal Chem 73: Neue UD (2005) J Chromatogr A 1079: Giddings JC (1967) Anal Chem 39: Horváth CG, Lipsky SR (1967) Anal Chem 39: Luo Q, Shen Y, Hixson KK, Zhao R, Yang F, Moore RJ, Mottaz HM, Smith RD (2005) Anal Chem 77: Stadalius MA, Quarry MA, Snyder LR (1985) J Chromatogr A 327: Snyder LR, Stadalius MA (1986) In: Horvath C (ed) Highperformance liquid chromatography, advances and perspectives. Academic, New York, pp Wang X, Barber WE, Carr PW (2006) J Chromatogr A 1107: Xiong L, Zhang R, Regnier F (2004) J Chromatogr A 1030: Hennessy TP, Boysen RI, Huber MI, Unger KK, Hearn MTW (2003) J Chromatogr A 1009: Leinweber FC, Schmid DG, Lubda D, Wiesmüller K, Jung G, Tallarek U (2003) Rapid Commun Mass Spectrom 17: Tolstikov VV, Lommen A, Nakanishi K, Tanaka N, Fiehn O (2003) Anal Chem 75: Eng JK, McCormack AL, Yates JR III (1994) J Am Soc Mass Spectrom 5: Perkins DN, Pappin DJC, Creasy DM, Cottrell JS (1999) Electrophoresis 20: Dolan JW, Snyder LR, Djordjevic NM, Hill DW, Waeghe TJ (1999) J Chromatogr A 857: Barroso B, Lubda D, Bischoff R (2003) J Protein Res 2: Rozenbrand J, van Bennekom WP, Unger KK, de Jong GJ (2006) Anal Bioanal Chem 385:
Characterization of Disulfide Linkages in Proteins by 193 nm Ultraviolet Photodissociation (UVPD) Mass Spectrometry. Supporting Information
Characterization of Disulfide Linkages in Proteins by 193 nm Ultraviolet Photodissociation (UVPD) Mass Spectrometry M. Montana Quick, Christopher M. Crittenden, Jake A. Rosenberg, and Jennifer S. Brodbelt
More information2D-LC as an Automated Desalting Tool for MSD Analysis
2D-LC as an Automated Desalting Tool for MSD Analysis Direct Mass Selective Detection of a Pharmaceutical Peptide from an MS-Incompatible USP Method Application Note Biologics and Biosimilars Author Sonja
More informationMaximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system
Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system A practical guide on how to use parameters to increase peak capacity Application Note Pharmaceutical and Chemical Author
More informationThe distribution of log 2 ratio (H/L) for quantified peptides. cleavage sites in each bin of log 2 ratio of quantified. peptides
Journal: Nature Methods Article Title: Corresponding Author: Protein digestion priority is independent of their abundances Mingliang Ye and Hanfa Zou Supplementary Figure 1 Supplementary Figure 2 The distribution
More informationEdgar Naegele. Abstract
Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast LC/TOF mass spectrometry Application ote Edgar aegele Abstract A recent trend in
More informationMass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System
Application Note Food Testing and Agriculture Mass-Based Purification of Natural Product Impurities Using an Agilent 126 Infinity II Preparative LC/MSD System Authors Florian Rieck and Jörg Hippler Agilent
More informationIncreasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 1290 Infinity LC System
Increasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 129 Infinity LC System Application Note Pharmaceutical and Chemical Analysis
More information4th Multidimensional Chromatography Workshop Toronto (January, 2013) Herman C. Lam, Ph.D. Calibration & Validation Group
4th Multidimensional Chromatography Workshop Toronto (January, 2013) Herman C. Lam, Ph.D. Calibration & Validation Group MDLC for Shotgun Proteomics Introduction General concepts Advantages Challenges
More informationPTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System
Application Note LC/MS PTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System Purpose This application note describes an automated workflow
More informationTransferring a Method for Analysis of DNPH-Derivatized Aldehydes and Ketones from HPLC to UHPLC
Transferring a Method for Analysis of DNPH-Derivatized Aldehydes and Ketones from HPLC to UHPLC Application Note Environmental Author Melanie Metzlaff Agilent Technologies, Inc. Waldbronn, Germany Abstract
More informationMS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine
Application Note: 346 MS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine Gargi Choudhary and Diane Cho, Thermo Fisher Scientific, San Jose, CA Wayne Skinner and
More informationHigh Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column
High Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column Application Note BioPharma Authors James Martosella, Phu Duong, and Alex Zhu Agilent Technologies, Inc. Abstract
More informationMaximizing Efficiency Using Agilent InfinityLab Poroshell 120 Columns
Maximizing Efficiency Using Agilent InfinityLab Poroshell 12 Columns 1, Plates in Less Than Minutes Using Coupled Column Technology Application Note Food, Environmental, Chemical, Pharmaceutical Authors
More informationApplication of a new capillary HPLC- ICP-MS interface to the identification of selenium-containing proteins in selenized yeast
Application of a new capillary HPLC- ICP-MS interface to the identification of selenium-containing proteins in selenized yeast Application note Food supplements Authors Juliusz Bianga and Joanna Szpunar
More informationIn-Solution Digestion for proteomics
In-Solution Digestion for proteomics Guidelines for sample preparation (How to protect your samples from contamination with keratin) 1. Try to avoid any contact of samples and solutions with dust, skin
More informationProtein and peptide separations,
A Quarterly Technical Newsletter Spring Grace Vydac: Leading the Way in Separations for Proteomics Protein and peptide separations, always important to protein chemists and enzymologists, have recently
More informationColumns for HPLC. Columns for HPLC
Basic principles of preparative HPLC Basically, preparative HPLC follows the same rules as analytical scale chromatography. However, there are important differences in the aims of the two techniques. In
More informationRITONAVIRI COMPRESSI RITONAVIR TABLETS. Final text for addition to The International Pharmacopoeia (July 2012)
July 2012 RITONAVIRI COMPRESSI RITONAVIR TABLETS Final text for addition to The International Pharmacopoeia (July 2012) This monograph was adopted at the Forty-sixth WHO Expert Committee on Specifications
More informationSUPPLEMENTARY MATERIAL
SUPPLEMENTARY MATERIAL Artepillin C, is it a good marker for quality control of Brazilian Green Propolis? Cui-ping Zhang 1, Xiao-ge Shen 1, Jia-wei Chen 1, Xia-sen Jiang 1, Kai Wang 2, Fu-liang Hu 1 *
More informationProteaseMAX Surfactant, Trypsin Enhancer
Technical Bulletin ProteaseMAX Surfactant, Trypsin Enhancer INSTRUCTIONS FOR USE OF PRODUCTS V2071 AND V2072. PRINTED IN USA. Revised 1/10 ProteaseMAX Surfactant, Trypsin Enhancer All technical literature
More informationTriptycene-Based Small Molecules Modulate (CAG) (CTG) Repeat Junctions
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2015 Triptycene-Based Small Molecules Modulate (CAG) (CTG) Repeat Junctions Stephanie A. Barros
More informationMass Spectrometry. Actual Instrumentation
Mass Spectrometry Actual Instrumentation August 2017 See also http://www.uni-bielefeld.de/chemie/analytik/ms f additional infmation 1. MALDI TOF MASS SPECTROMETRY ON THE ULTRAFLEX 2 2. ESI MASS SPECTROMETRY
More informationNeosolaniol. [Methods listed in the Feed Analysis Standards]
Neosolaniol [Methods listed in the Feed Analysis Standards] 1 Simultaneous analysis of mycotoxins by liquid chromatography/ tandem mass spectrometry [Feed Analysis Standards, Chapter 5, Section 1 9.1 ]
More informationAnalysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF
Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF Application Note 219 Joan Stevens, PhD; Luke Roenneburg; Kevin Fawcett (Gilson,
More informationEffective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate
Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate Application Note Pharmaceutical QA/QC Author Siji Joseph Agilent Technologies, Inc. Bangalore,
More informationTENOFOVIR TABLETS: Final text for addition to The International Pharmacopoeia (June 2010)
June 2010 TENOFOVIR TABLETS: Final text for addition to The International Pharmacopoeia (June 2010) This monograph was adopted at the Forty-fourth WHO Expert Committee on Specifications for Pharmaceutical
More informationTrypsin Mass Spectrometry Grade
058PR-03 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Trypsin Mass Spectrometry Grade A Chemically Modified, TPCK treated, Affinity Purified
More informationComputer-Assisted Optimization of a Gradient HPLC Method for the Separation of Flavonoids
25 Computer-Assisted Optimization of a Gradient HPLC Method for the Separation of Flavonoids Tomasz Baczek, Grazyna Lewandowska, Roman Kaliszan, Department of Biopharmaceutics and Pharmacodynamics, Medical
More informationShotgun Proteomics MS/MS. Protein Mixture. proteolysis. Peptide Mixture. Time. Abundance. Abundance. m/z. Abundance. m/z 2. Abundance.
Abundance Abundance Abundance Abundance Abundance Shotgun Proteomics Protein Mixture 1 2 3 MS/MS proteolysis m/z 2 3 Time µlc m/z MS 1 m/z Peptide Mixture m/z Block Diagram of a Mass Spectrometer Sample
More informationAntoine Bouchoux, Pierre-Emerson Cayemitte, Julien Jardin, Geneviève Gésan-Guiziou, and Bernard Cabane
Biophysical Journal, Volume 96 Supplementary Material Casein Micelle Dispersions under Osmotic Stress Antoine Bouchoux, Pierre-Emerson Cayemitte, Julien Jardin, Geneviève Gésan-Guiziou, and Bernard Cabane
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/NCHEM.2419 Diversification of Self-Replicating Molecules Jan W. Sadownik, Elio Mattia, Piotr Nowak, Sijbren Otto* University of Groningen, Center for Systems Chemistry, Stratingh Institute
More informationTargeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur
Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS Kayleigh Arthur K.Arthur@lboro.ac.uk Introduction LC-MS is a highly used technique for untargeted profiling analyses
More informationAuthors. Abstract. Introduction. Environmental
Determination of Ultratrace Amitrol in Water Samples by in situ Derivatization-Solid Phase Extraction-Liquid Chromatography-Mass Selective Detector Application Environmental Authors Gerd Vanhoenacker,
More informationAutomated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222
Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Joan Stevens, Ph.D.; Luke Roenneburg; Tim Hegeman; Kevin Fawcett
More informationQuantification of lovastatin in human plasma by LC/ESI/MS/MS using the Agilent 6410 Triple Quadrupole LC/MS system
Quantification of lovastatin in human plasma by LC/ESI/MS/MS using the Agilent 641 Triple Quadrupole LC/MS system Application Note Clinical Research Author Siji Joseph Agilent Technologies Bangalore, India
More informationApplication of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices
Application ote #LCMS-2 esquire series Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices Introduction The simple monitoring
More informationLecture 3. Tandem MS & Protein Sequencing
Lecture 3 Tandem MS & Protein Sequencing Nancy Allbritton, M.D., Ph.D. Department of Physiology & Biophysics 824-9137 (office) nlallbri@uci.edu Office- Rm D349 Medical Science D Bldg. Tandem MS Steps:
More informationLC/MS Method for Comprehensive Analysis of Plasma Lipids
Application Note omics LC/MS Method for Comprehensive Analysis of Plasma s Authors Tomas Cajka and Oliver Fiehn West Coast Metabolomics Center, University of California Davis, 451 Health Sciences Drive,
More informationPHOSPHOPEPTIDE ANALYSIS USING IMAC SAMPLE PREPARATION FOLLOWED BY MALDI-MS and MALDI PSD MX
PHOSPHOPEPTIDE ANALYSIS USING IMAC SAMPLE PREPARATION FOLLOWED BY MALDI-MS and MALDI PSD MX E. Claude 1, E. Emekwue 2, M. Snel 1, T. McKenna 1 and J. Langridge 1 1: Waters Corporation, Manchester, UK 2:
More informationAgilent Protein In-Gel Tryptic Digestion Kit
Agilent 5188-2749 Protein In-Gel Tryptic Digestion Kit Agilent Protein In-Gel Tryptic Digestion Kit Instructions Kit Contents The Protein In-Gel Tryptic Digestion Kit includes sufficient reagents for approximately
More informationAPPLICATIONS Improving Intact Biogeneric Protein Separations with Aeris WIDEPORE Core-Shell Columns
Improving Intact Biogeneric Protein Separations with Aeris WIDEPORE Core-Shell Columns Michael McGinley, Deborah Jarrett, and Jeff Layne Phenomenex, Inc., 411 Madrid Avenue, Torrance, CA 91 USA Aeris WIDEPORE
More informationZIDOVUDINE, LAMIVUDINE AND ABACAVIR TABLETS Draft proposal for The International Pharmacopoeia (September 2006)
September 2006 RESTRICTED ZIDOVUDINE, LAMIVUDINE AND ABACAVIR TABLETS Draft proposal for The International Pharmacopoeia (September 2006) This document was provided by a contracted quality control laboratory.
More informationUsing Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies.
Using Software Tools to Improve the Detection of Impurities Application Note Christine Miller Introduction The analysis of raw materials and finished products for or impurities presents a challenge in
More informationHeparin Sodium ヘパリンナトリウム
Heparin Sodium ヘパリンナトリウム Add the following next to Description: Identification Dissolve 1 mg each of Heparin Sodium and Heparin Sodium Reference Standard for physicochemical test in 1 ml of water, and
More informationApplication Note # ET-17 / MT-99 Characterization of the N-glycosylation Pattern of Antibodies by ESI - and MALDI mass spectrometry
Bruker Daltonics Application Note # ET-17 / MT-99 Characterization of the N-glycosylation Pattern of Antibodies by ESI - and MALDI mass spectrometry Abstract Analysis of the N-glycosylation pattern on
More informationSeparation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement
Separation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement Application Note Food Testing & Agriculture Author Edgar Naegele Agilent Technologies,
More informationRapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit
Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit Application Note General Chromatography, Food Industry Authors John W. Henderson Jr., Judy Berry, Anne Mack, William Long Agilent
More informationComparison of mass spectrometers performances
Comparison of mass spectrometers performances Instrument Mass Mass Sensitivity resolution accuracy Quadrupole 1 x 10 3 0.1 Da* 0.5-1.0 pmol DE-MALDI 2 x 10 4 20 ppm 1-10 fmol peptide 1-5 pmol protein Ion
More informationNew Solvent Grade Targeted for Trace Analysis by UHPLC-MS
New Solvent Grade Targeted for Trace Analysis by UHPLC-MS Subhra Bhattacharya, Deva H. Puranam, and Stephen C. Roemer Thermo Fisher Scientific Fisher Chemical, One Reagent Lane, Fair Lawn, NJ Material
More informationUltra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification
22 SEPARATION SCIENCE REDEFINED MAY 2005 Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification In the drug discovery process the detection and
More informationTrypsin Digestion Mix
G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name 239PR Trypsin Digestion Mix Provides optimal buffered conditions for in gel trypsin digestion
More informationGradient Elution LC-MS/MS Determination of Gefitinib in Rat Plasma and its Pharmacokinetic Study
Latin American Journal of Pharmacy (formerly Acta Farmacéutica Bonaerense) Lat. Am. J. Pharm. 32 (2): 292-6 (2013) Short Communication Received: November 20, 2012 Accepted: December 6, 2012 Gradient Elution
More informationSupporting Information
Notes Bull. Korean Chem. Soc. 2013, Vol. 34, No. 1 1 http://dx.doi.org/10.5012/bkcs.2013.34.1.xxx Supporting Information Chemical Constituents of Ficus drupacea Leaves and their α-glucosidase Inhibitory
More informationBioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS
Bioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS Jenny Chen, Hongxia Wang, Zhiqi Hao, Patrick Bennett, and Greg Kilby Thermo Fisher
More informationQuantitation of Protein Phosphorylation Using Multiple Reaction Monitoring
Quantitation of Protein Phosphorylation Using Multiple Reaction Monitoring Application Note Authors Ning Tang, Christine A. Miller and Keith Waddell Agilent Technologies, Inc. Santa Clara, CA USA This
More informationApplication Note. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice. Author. Abstract.
Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice Application Note Author Food Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India 8 6 4 2
More informationSupporting information
Supporting information Figure legends Supplementary Table 1. Specific product ions obtained from fragmentation of lithium adducts in the positive ion mode comparing the different positional isomers of
More informationProfiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography
Profiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography Application Note Metabolomics Author Yuqin Dai Agilent Technologies, Inc. 31 Stevens Creek Blvd,
More informationO O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions
Simplifying Qual/Quan Analysis in Discovery DMPK using UPLC and Xevo TQ MS Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION The determination of the drug metabolism
More informationAnalytical Method for 2, 4, 5-T (Targeted to Agricultural, Animal and Fishery Products)
Analytical Method for 2, 4, 5-T (Targeted to Agricultural, Animal and Fishery Products) The target compound to be determined is 2, 4, 5-T. 1. Instrument Liquid Chromatograph-tandem mass spectrometer (LC-MS/MS)
More informationQuantitative chromatin proteomics reveals a dynamic histone. post-translational modification landscape that defines asexual
Quantitative chromatin proteomics reveals a dynamic histone post-translational modification landscape that defines asexual and sexual Plasmodium falciparum parasites Nanika Coetzee 1, Simone Sidoli 2,
More informationIn-Gel Tryptic Digestion Kit
INSTRUCTIONS In-Gel Tryptic Digestion Kit 3747 N. Meridian Road P.O. Box 117 Rockford, IL 61105 89871 1468.2 Number Description 89871 In-Gel Tryptic Digestion Kit, sufficient reagents for approximately
More informationThiol-Activated gem-dithiols: A New Class of Controllable. Hydrogen Sulfide (H 2 S) Donors
Thiol-Activated gem-dithiols: A New Class of Controllable Hydrogen Sulfide (H 2 S) Donors Yu Zhao, Jianming Kang, Chung-Min Park, Powell E. Bagdon, Bo Peng, and Ming Xian * Department of Chemistry, Washington
More informationLC/MS/MS Separation of Cholesterol and Related Sterols in Plasma on an Agilent InfinityLab Poroshell 120 EC C18 Column
Application Note Clinical Research LC/MS/MS Separation of Cholesterol and Related Sterols in Plasma on an Agilent InfinityLab Poroshell EC C8 Column Authors Rongjie Fu and Zhiming Zhang Agilent Technologies
More informationImpact of Chromatography on Lipid Profiling of Liver Tissue Extracts
Impact of Chromatography on Lipid Profiling of Liver Tissue Extracts Application Note Clinical Research Authors Mark Sartain and Theodore Sana Agilent Technologies, Inc. Santa Clara, California, USA Introduction
More informationSupporting information
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Supporting information Glycan Reductive Isotope-coded Amino Acid Labeling (GRIAL) for Mass Spectrometry-based
More informationCHAPTER-6 IDENTIFICATION, AND CHARACTERISATION OF DEGRADATION IMPURITY IN VALSARTAN TABLETS
129 CHAPTER-6 IDENTIFICATION, AND CHARACTERISATION OF DEGRADATION IMPURITY IN VALSARTAN TABLETS 130 6.1. Introduction Valsartan is an orally active specific angiotensin II blocker effective in lowering
More informationMass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector
Mass Spectrometry MALDI-TOF ESI/MS/MS Mass spectrometer Basic components Ionization source Mass analyzer Detector 1 Principles of Mass Spectrometry Proteins are separated by mass to charge ratio (limit
More informationQualitative and quantitative determination of phenolic antioxidant compounds in red wine and fruit juice with the Agilent 1290 Infinity 2D-LC Solution
Qualitative and quantitative determination of phenolic antioxidant compounds in red wine and fruit juice with the Agilent 1290 Infinity 2D-LC Solution Application Note Food Testing Author Edgar Naegele
More informationDevelopment of a Cell-penetrating Peptide that Exhibits Responsive. Changes in its Secondary Structure in the Cellular Environment
Development of a Cell-penetrating Peptide that Exhibits Responsive Changes in its Secondary Structure in the Cellular Environment iroko Yamashita, 1 Takuma Kato, 2 Makoto ba, 2 Takashi Misawa, 1 Takayuki
More informationA Robustness Study for the Agilent 6470 LC-MS/MS Mass Spectrometer
A Robustness Study for the Agilent 7 LC-MS/MS Mass Spectrometer Application Note Clinical Research Authors Linda Côté, Siji Joseph, Sreelakshmy Menon, and Kevin McCann Agilent Technologies, Inc. Abstract
More informationMultiplex Protein Quantitation using itraq Reagents in a Gel-Based Workflow
Multiplex Protein Quantitation using itraq Reagents in a Gel-Based Workflow Purpose Described herein is a workflow that combines the isobaric tagging reagents, itraq Reagents, with the separation power
More informationApplication Note. Abstract. Authors. Pharmaceutical
Analysis of xycodone and Its Metabolites-oroxycodone, xymorphone, and oroxymorphone in Plasma by LC/MS with an Agilent ZRBAX StableBond SB-C18 LC Column Application ote Pharmaceutical Authors Linda L.
More informationMASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010
MASS SPECTROMETRY BASED METABOLOMICS Pavel Aronov ABRF2010 Metabolomics Research Group March 21, 2010 Types of Experiments in Metabolomics targeted non targeted Number of analyzed metabolites is limited
More informationIdentification of Steroids in Water by Ion Trap LC/MS/MS Application
Identification of Steroids in Water by Ion Trap LC/MS/MS Application Environmental Author Paul Zavitsanos and Christine Miller Agilent Technologies, Inc. Centerville Road Wilmington, DE 9- USA Abstract
More information4-Plex itraq Based Quantitative Proteomic Analysis Using an Agilent Accurate -Mass Q-TOF
4-Plex itraq Based Quantitative Proteomic Analysis Using an Agilent Accurate -Mass Q-TOF Application Note Authors H. C. Harsha, G. S. S. Kumar, and A. Pandey Institute of Bioinformatics Bangalore India
More informationSupporting Information for: Daniel Knappe, Stefania Piantavigna, Anne Hansen, Adam Mechler, Annegret Binas,
Supporting Information for: Oncocin (VDKPPYLPRPRPPRRIYNR-NH 2 ): a novel antibacterial peptide optimized against Gram-negative human pathogens Daniel Knappe, Stefania Piantavigna, Anne Hansen, Adam Mechler,
More informationRP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms
Asian Journal of Chemistry Vol. 22, No. 7 (2010), 5067-5071 RP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms A. LAKSHMANA RAO*, G. TARAKA RAMESH and J.V.L.N.S. RAO Department of Pharmaceutical
More informationDetermination of Aflatoxins in Food by LC/MS/MS. Application. Authors. Abstract. Experimental. Introduction. Food Safety
Determination of Aflatoxins in Food by LC/MS/MS Application Food Safety Authors Masahiko Takino Agilent Technologies 9-1 Takakura-Cho Hachiouji-Shi, Tokyo Japan Toshitsugu Tanaka Kobe Institute of Health
More informationCannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system
Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 9 Infinity II/63B LC/TOF system Application Brief Authors Mike Adams, Karen Kaikaris, and A. Roth CWC Labs Joan Stevens, Sue D
More informationIncreased Identification Coverage and Throughput for Complex Lipidomes
Increased Identification Coverage and Throughput for Complex Lipidomes Reiko Kiyonami, David Peake, Yingying Huang, Thermo Fisher Scientific, San Jose, CA USA Application Note 607 Key Words Q Exactive
More informationPosterREPRINT A NOVEL APPROACH TO MALDI-TOF-MS SAMPLE PREPARATION. Presented at ABRF 2002, Austin, Texas, USA, 9th - 12th March 2002.
Introduction A NOVEL APPROACH TO MALDI-TOF-MS SAMPLE PREPARATION Ed Bouvier 2, Jeff Brown 1, Emmanuelle Claude 1, John L. Gebler 2, Weibin Chen 2, *Dominic Gostick 1, Kevin Howes 1, James Langridge 1,
More informationSolid Phase Peptide Synthesis (SPPS) and Solid Phase. Fragment Coupling (SPFC) Mediated by Isonitriles
Solid Phase Peptide Synthesis (SPPS) and Solid Phase Fragment Coupling (SPFC) Mediated by Isonitriles Ting Wang a and Samuel J. Danishefsky a,b,* alaboratory for Bioorganic Chemistry, Sloan- Kettering
More informationCharged Surface Hybrid C18 for High Resolution LC and LC/MS Peptide Separations
Charged Surface Hybrid C18 for High Resolution LC and LC/MS Peptide Separations Higher Quality Peptide Separations Using Hybrid Particle-Based Reversed-Phase Columns and CSH Technology Matthew Lauber,
More informationProteomics Grade. Protocol. Catalog # Agilent Technologies. Research Use Only. Not for use in Diagnostic Procedures. Version A, January 2010
Proteomics Grade Trypsin Catalog #204310 Protocol Version A, January 2010 Research Use Only. Not for use in Diagnostic Procedures. Agilent Technologies Notices Agilent Technologies, Inc. 2010 No part of
More informationQuantitative Analysis of Underivatized Amino Acids in Plant Matrix by Hydrophilic Interaction Chromatography (HILIC) with LC/MS Detection
Application Note Food Testing, Metabolomics, Agricultural Chemistry, Environmental Quantitative Analysis of Underivatized Amino Acids in Plant Matrix by Hydrophilic Interaction Chromatography (HILIC) with
More informationSupplementary information Oxaliplatin reacts with DMSO only in the presence of water
Electronic Supplementary Material (ESI) for Dalton Transactions. This journal is The Royal Society of Chemistry 2017 Supplementary information Oxaliplatin reacts with DMSO only in the presence of water
More informationAnalysis of Counterfeit Antidiabetic Drugs by UHPLC with the Agilent 1220 Infinity Mobile LC
Analysis of Counterfeit Antidiabetic Drugs by UPLC with the Agilent 1 Infinity Mobile LC Application Note Small Molecule Pharmaceuticals & Generics Author Sonja Schneider Agilent Technologies, Inc. Waldbronn,
More informationStudies on Stationary Phase Selectivity for Solid-Core Particles
Studies on Stationary Phase Selectivity for Solid-Core Particles Richard A. enry, Wayne K. Way, Carmen T. Santasania, and David S. Bell Supelco, Div. of Sigma-Aldrich, Bellefonte, PA 6823 USA T4060 sigma-aldrich.com
More informationApplication Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform
Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Abstract Targeted proteomics for biomarker verification/validation
More informationEnhancing Sequence Coverage in Proteomics Studies by Using a Combination of Proteolytic Enzymes
Enhancing Sequence Coverage in Proteomics Studies by Using a Combination of Proteolytic Enzymes Dominic Baeumlisberger 2, Christopher Kurz 3, Tabiwang N. Arrey, Marion Rohmer 2, Carola Schiller 3, Thomas
More informationSeQuant ZIC -HILIC For all who expect more...
SeQuant ZIC -ILIC For all who expect more... The better choice for PLC and LC/MS of all types of polar and hydrophilic compounds EMD Millipore Corp. is a subsidiary of Merck KGaA, Darmstadt, Germany Poor
More informationSmall Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application
Small Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application Pharmaceutical Author Cliff Woodward Agilent Technologies, Inc. 285 Centerville
More informationAnalytical Method Development for USP Related Compounds in Paclitaxel Using an Agilent Poroshell 120 PFP
Analytical Method Development for USP Related Compounds in Paclitaxel Using an Agilent Poroshell 1 PFP Application Note Clinical Research Author Rongjie Fu Agilent Technologies (Shanghai) Co. Ltd Abstract
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2015, 6(1):6-10 ISSN: 0976-8688 CODEN (USA): PSHIBD Validated RP-HPLC method for simultaneous estimation of metformin hydrochloride
More informationLipidomic Analysis by UPLC-QTOF MS
Lipidomic Analysis by UPLC-QTOF MS Version: 1 Edited by: Oliver Fiehn Summary Reagents and Materials Protocol Summary:Lipidomic analysis by UPLC-QTOF mass spectrometry Reagents and Materials: Reagent/Material
More informationDry eye disease commonly known as atopic keratoconjunctivitis is an autoimmune disease of
4.1. Introduction Dry eye disease commonly known as atopic keratoconjunctivitis is an autoimmune disease of eyes. The disease is characterized by lesser or some time no-significant production of tear;
More informationSupporting Information. 58 Pages. 6 Figures 4 Tables
Light-Source-Dependent Effects of Main Water Constituents on Photodegradation of Phenicol Antibiotics: Mechanism and Kinetics Linke Ge, Jingwen Chen, * Xianliang Qiao, Jing Lin, Xiyun Cai Key Laboratory
More informationAnalysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column
Application Note Pharmaceutical and Food Testing Analysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column Author Lu Yufei Agilent Technologies, Inc. Abstract A liquid chromatographic
More informationLABORATÓRIUMI GYAKORLAT SILLABUSZ SYLLABUS OF A PRACTICAL DEMONSTRATION. financed by the program
TÁMOP-4.1.1.C-13/1/KONV-2014-0001 projekt Az élettudományi-klinikai felsőoktatás gyakorlatorientált és hallgatóbarát korszerűsítése a vidéki képzőhelyek nemzetközi versenyképességének erősítésére program
More information