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1 Pyrus boissieriana Buhse HPLC محمد ا زادبخت(. Ph.D )* محمد رمضاني **(Ph.D.) مريم جهرمي مقدم(. Ph.D )*** چكيده سابقه و هدف : (Pyrus boissieriana Buhse).. Pyrus.... Ericaceae,Rosaceae.. مواد و روش ها : TLC HPLC. HPLC يافتهها : Arctostaphylos uva-ursi. / / Vaccinium myrtillus / Vaccinium vitis /. / / ( ) استنتاج : V. vitis A. uva-ursi. واژه هاي آليدي :.. مقدمه Pyrus boissieriana Buhse - : *** // :, * ** // : // : 1 مجله علمي- پژوهشي دانشگاه علوم پزشكي مازندران

2 تعيين مقدار ا ربوتين در برگ گياه تلكا..(). Pyrus.() (Arctostaphylos uva-ursi),. مواد و روش ها :(Merck ) ( -. ) : : Genesis2 : Rotary Evaporator (nm : : Laborata 4000: ) : : ) Freeze drying ( : : Zirbus: : ) ( - : : Desaga Knauer: )HPLC (nm ( ) : Shimadzue HPLC Pump K-1001 UV Detector K-2600 Eurospher- : (:) :...() Pyrus.() ().()... Ericaceae,Rosaceae Pyrus.(). Pyrus / C 12 H 16 O 7 nm..() :. 1. Arbutin 2. Whiting agent 2 مجله علمي- پژوهشي دانشگاه علوم پزشكي مازندران

3 . :HPLC ( ppm / ) ( ppm ). HPLC ( / /) ppm HPLC grade (...(). : ( / /.. / - /.. : C 18 : nm :.() (flow rate: 1 ml/ min : (. : (. Rotary evaporator HPLC : ( TLC. TLC. TLC /) (. ( : / :). ( ).() ( RT ) HPLC.. HPLC (Additional standard ) 4. Internal Standard 5. Emerson Reaction 1. High Performance liquid chromato graphy 2. Thin layer chromatography 3. Retention time 3 مجله علمي- پژوهشي دانشگاه علوم پزشكي مازندران سال سيزدهم/ شماره ٤٠/ پاييز ١٣٨٢

4 تعيين مقدار ا ربوتين در برگ گياه تلكا. ( ) Eurospher 100- C 18 : : HPLC ( : ) : : : : : : nm A.U.F.S= / ml/min: Knauer. / ml/min Shimadzue : ( ) ppm HPLC ( ) ( ) (. ( ). nm.() يافتهها TLC ( R f. R f / R f ) R f (. ). ( ( HPLC ( ) ). ( / / 4 مجله علمي- پژوهشي دانشگاه علوم پزشكي مازندران

5 : ( ) : ( ) ppm,. ( /,. / /,. / / /.. / : ( ppm : ) : ( : HPLC % Area Area(mV*min) RT (min) (ppm ) (ppm) (ppm ) (ppm) (ppm ) (ppm) (ppm ) (ppm) (ppm ) (ppm) (ppm) 5 مجله علمي- پژوهشي دانشگاه علوم پزشكي مازندران سال سيزدهم/ شماره ٤٠/ پاييز ١٣٨٢

6 تعيين مقدار ا ربوتين در برگ گياه تلكا.() () Harborne GLC TLC,HPLC () Lubsandorzhieva.(), () Petricic.() Sticher, () Sun () Schieber ().(,,,) HPLC TLC HPLC HPLC. Arctostaphilos uva-ursi / Pyrus piraster / Pyrus amigdaliformis / Bergenia crossifolia.() Vaccinium vitis. % / Pyrus biossieriana /,,. HPLC / HPLC. (E 1 = )., HPLC., = *E*/ b**e 1 = E / b*e 1 =E = E 1. =b, b E 1,.() = / * E E=/. بحث.,.(),...(),., Pyrus 6 مجله علمي- پژوهشي دانشگاه علوم پزشكي مازندران

7 / HPLC..,..١.- 2. Petricic J, Apostolski R, Srepel B. The leaf of the wild pear-tree as an arbutinic herb. Farmacevtski Vestnik Ljubljana Journal, 1981; 32(2): Lubsandorzhieva P.B, Zhigzhitov B.S, Dargaeva Zh.G, Nagaslaeva L.A. Chromatospectrophotometric determination of arbutin in the leaves of Bergenia crassifolia (L.). Pharmaceutical Chemistry Journal, 2000; 34(5): pp Couteau C, Coifard L. Photostability determination of arbutin, a vegetable whitening agent. Farmaco Journal, 2000; 55(5): Bilia A.R, Rubio M, Alvarez M, Morelli I, Gonzalez M. New benzyl alcohol glycosides from Pyrus bourgaeana. Planta Medica Journal, 1994; 60(6): Sticher O, Soldati F, Lehmann D. HPLC separation and quantitive determination of arbutin, methyl arbutin, hydroquinone and hydroquinone monomethylether in Arctostaphylos, Bergenia, Callena and Vaccinium species. Planta Medica Journal, 1994; 60(6): Wagner H, Bladt S. Drugs containing arbutin, salicin and salycoil derivatives. فهرست منابع In Plant Drug Analysis. 2 th ed. Berlin: Springer. 1996: ٨ : Stambergova A, Supcikova M, Leifetova I. Evaluation of phenolic substances in Arctostaphylos uva ursi, determination of arbutin, methylarbutin and hydroquinone in the leaves by HPLC.Ceska-a-slovenskafarmacie, 1985; 34(5): Harborne J.B. Phenolic compounds. In Phytochemical methods, 3 th ed, London: Chapman and Hall. 1998: Schieber A, Keller P, Carle R. Determination of phrnolic asids and flavonoids of apple and pear by HPLC. Journal of Chromatography, 2001; 910(2): Sun H, Wang X, Huang R, Yuan C. Determinatiom of arbutin in the herbs of Vaccinium vitis-idea by HPLC. Zhongguo-zhong-yao-za-zhi, 1997; 22(9): مجله علمي- پژوهشي دانشگاه علوم پزشكي مازندران سال سيزدهم/ شماره ٤٠/ پاييز ١٣٨٢