Sphingomonas yabuuchiae sp. nov. and Brevundimonas nasdae sp. nov., isolated from the Russian space laboratory Mir
|
|
- Virginia Harvey
- 6 years ago
- Views:
Transcription
1 International Journal of Systematic and Evolutionary Microbiology (2004), 54, DOI /ijs Sphingomonas yabuuchiae sp. nov. and Brevundimonas nasdae sp. nov., isolated from the Russian space laboratory Mir Ying Li, 1 Yoshiaki Kawamura, 1 Nagatoshi Fujiwara, 2 Takashi Naka, 2 Hongsheng Liu, 1 Xinxiang Huang, 1 Kazuo Kobayashi 2 and Takayuki Ezaki 1 Correspondence Takayuki Ezaki tezaki@cc.gifu-u.ac.jp 1 Department of Microbiology Bioinformatics, Regeneration and Advanced Medical Science, Gifu University, Graduate School of Medicine, Tsukasa-machi 40, Gifu , Japan 2 Department of Bacteriology, Osaka City University, Medical School, Abeno-ku, Osaka, , Japan On the basis of phenotypic and genotypic characteristics and 16S rrna gene sequence analysis, novel species belonging to the genera Sphingomonas and Brevundimonas were identified from samples taken from the Russian space laboratory Mir. Strain A1-18 T was isolated from the air. 16S rdna sequence analysis showed that strain A1-18 T formed a coherent cluster with Sphingomonas sanguinis, Sphingomonas parapaucimobilis, Sphingomonas paucimobilis and Sphingomonas roseiflava with sequence similarity of 97?5 98?6 %. Similar to other Sphingomonas species, the G+C content was 66?1 mol%, but DNA DNA hybridization rates at optimal temperatures among these related species were only 24?7 51?7 %. Strain A1-18 T can be differentiated biochemically from related species. Strain W1-2B T was isolated from condensation water. It forms a distinct lineage within the genus Brevundimonas, forming a coherent cluster with Brevundimonas vesicularis, Brevundimonas aurantiaca and Brevundimonas intermedia. 16S rdna sequence similarities were 98?6 99?5 % and the G+C content was 66?5 mol%, similar to other Brevundimonas species, but DNA DNA relatedness was only 50?2 54?8%. Strain W1-2B T also showed some differential biochemical properties from its related species. A series of polyphasic taxonomic studies led to the proposal of two novel species, Sphingomonas yabuuchiae sp. nov. (type strain A1-18 T =GTC 868 T =JCM T =DSM T ) and Brevundimonas nasdae sp. nov. (type strain W1-2B T =GTC 1043 T =JCM T =DSM T ). INTRODUCTION Exploring space is a challenge for human beings. The space station is a special closed environment where astronauts are constantly faced with the unfavourable effects of space flight and where medical treatment facilities are extremely limited. Basic data on the bacterial microflora inside the space station are essential to manage the astronauts health. As part of an international research effort between Japan and Russia, we investigated the bacterial microflora from condensation water and air on the Russian space laboratory Mir in The bacterial population in the space station has been reported previously by Kawamura et al. (2001). In this study, we determined the taxonomic status of two Published online ahead of print on 12 December 2003 as DOI /ijs The GenBank/EMBL/DDBJ accession numbers for the 16S rdna sequences of S. yabuuchiae sp. nov. A1-18 T and B. nasdae sp. nov. W1-2B T are AB and AB Detailed DNA DNA hybridization results are available as supplementary data in IJSEM Online. Gram-negative isolates that belong to the a-proteobacteria. Polyphasic taxonomic study of the isolates showed that they belonged to the genera Sphingomonas and Brevundimonas, both affiliated to the a-proteobacteria (Garrity & Holt, 2001), but neither isolate could be classified as any existing species. The genus Sphingomonas was proposed by Yabuuchi et al. (1990). Members of the genus are non-fermentative, Gram-negative, non-motile or motile rods and are characterized by the presence of 2-hydroxymyristic acid (2-OH 14 : 0), by the absence of 3-hydroxy fatty acids and by the presence of ubiquinone 10 (Q10). The genus Brevundimonas was proposed by Segers et al. (1994). All strains are characterized by two major fatty acids, 16 : 0 and 18 : 1, and the G+C content ranges from 65 to 68 mol% (Segers et al., 1994). In this study, we used morphological, biochemical, chemotaxonomic and genetic data to classify these two strains and propose two novel species, Sphingomonas yabuuchiae sp. nov. and Brevundimonas nasdae sp. nov G 2004 IUMS Printed in Great Britain 819
2 Y. Li and others METHODS Sample collection, strains, culture and DNA extraction. Air samples from the living environment of the Mir station were collected by an air vacuum sampler 16 and 3 days before the end of the mission and trapped on Millipore HA filters (0?45 mm pore size). The filters were placed in TGE medium (l 21 : 5?0 g beef extract, 10?0 g tryptone, 2?0 g glucose, 1 g triphenyl tetrazolium) and kept at room temperature under aerobic conditions for 7 days and then stored at 2 8 uc until the return to Earth. Three samples of condensation water were collected in different rooms 3 days before the end of the mission and stored at 2 8 uc. All samples were cultured directly on various selected media at 30 and 37 uc under aerobic, 5 % CO 2 and anaerobic conditions for 3 days to 4 weeks after transfer to the laboratory. Single colonies were transferred to brain heart infusion (BHI) agar plates and incubated at 30 or 37 uc. The following type strains were cultured on BHI agar plates at 30 uc: Sphingomonas sanguinis GTC 829 T (=IFO T ), Sphingomonas parapaucimobilis GTC 413 T (=JCM 7570 T ), Sphingomonas paucimobilis Gifu 2395 T (=ATCC T ), Sphingomonas trueperi EY 4281 T (=ATCC T ), Sphingomonas roseiflava GTC 1968 T (=IAM T ), Sphingomonas pituitosa EY 4370 T (=DSM T ), Brevundimonas intermedia GTC 1682 T (=DSM 4732 T ), Brevundimonas aurantiaca GTC 1681 T (=DSM 4731 T ), Brevundimonas diminuta GTC 14 T (=NCTC 8548 T ) and Brevundimonas vesicularis Gifu 2388 T (=ATCC T ). DNA was extracted as described previously (Ezaki et al., 1994). Sequencing of the 16S rrna gene, phylogenetic analysis and determination of DNA composition. The 16S rrna gene was amplified with universal primers and PCR products for all isolates were sequenced in both directions. The sequences were analysed with the FASTA search system (Pearson & Lipman, 1988) on the DDBJ website ( to find closely related bacterial 16S rdna sequences. CLUSTAL W software (Thompson et al., 1994) was also used to determine the phylogenetic relationships of the isolates. Phylogenetic trees were produced according to the neighbour-joining method (Saitou & Nei, 1987) and visualized using TreeView (Page, 1996). G+C content was measured by HPLC as described previously (Ezaki et al., 1990). Escherichia coli was used as a standard (G+C content 51?19 mol%). RESULTS AND DISCUSSION Thirty-six bacterial colonies were isolated from air samples and bacteria were found at concentrations of 2?1610 6, 5? and 3? c.f.u. ml 21 in three condensation water samples (Kawamura et al., 2001). After performing morphological observations, we selected 16 and seven representative isolates from air samples and condensation water samples, respectively, to characterize further. 16S rdna sequence similarity analysis showed that strain A1-18 T and W1-2B T were affiliated with two genera within the a-proteobacteria, Sphingomonas and Brevundimonas, respectively. Finally, A1-18 T and W1-2B T were confirmed to represent distinct species after assessment of their taxonomic status by polyphasic taxonomic study. Strain A1-18 T Strain A1-18 T was isolated from an air sample. 16S rdna sequence analysis showed that strain A1-18 T formed a coherent cluster with S. sanguinis, S. parapaucimobilis, S. paucimobilis and S. roseiflava (Fig. 1). 16S rdna sequence similarity was determined to be 98?6, 98?4, 97?5 and 98?3 %, respectively, by CLUSTAL W analysis and the similarity to other members of genus Sphingomonas was lower than 97 %. The DNA G+C content of strain A1-18 T (66?1 mol%) was within the range of values (61?6 67?8 mol%) reported for established Sphingomonas species Determination of biochemical characteristics. Biochemical characterization of these strains was performed using the Biolog GN2 MicroPlate assay, Nonfergram S-1 kit (Wako Chemical) and API 20 NE (API biomérieux). Preparations were performed according to the manufacturers instructions. Oxidase activity was determined with Oxidase test strips (Eiken Chemical). Catalase activity was estimated by the production of bubbles from 3 % hydrogen peroxide solutions. Determination of isoprenoid quinones and cellular fatty acids. Isoprenoid quinone analysis was performed as previously described (Yano et al., 1987). Fatty acid methyl esters were extracted and prepared using the standard protocol for the MIDI/Hewlett Packard Microbial Identification System. Fatty acid methyl ester extracts were analysed using a Hewlett Packard GC (model HP6890) equipped with a flame-ionization detector, an automatic sampler, an integrator and a computer, as described by Kämpfer & Kroppenstedt (1996). DNA DNA hybridization. Quantitative microplate DNA DNA hybridization for selected strains was carried out as described previously (Ezaki et al., 1989). Hybridization experiments were carried out under optimal and stringent temperatures calculated from the melting temperatures (T m ) based on the G+C content of each test strain. Fig. 1. Phylogenetic position of two Gram-negative isolates from the Mir space station and selected members of the genera Sphingomonas and Brevundimonas based on 16S rdna sequences. Distances were calculated by the neighbourjoining method. Numbers at branch points are bootstrap values (based on 1000 samplings). GenBank accession numbers are given in parentheses. Bar, 0?01 estimated substitutions per nucleotide position. Arthrobacter oxydans was used as an outgroup. 820 International Journal of Systematic and Evolutionary Microbiology 54
3 Novel Sphingomonas and Brevundimonas species from Mir (Denner et al., 2001). Therefore, strain A1-18 T is affiliated phylogenetically with the genus Sphingomonas. Strain A1-18 T showed some similar morphological properties to Sphingomonas species (see species description). Some common biochemical properties with related species (shown in Table 1) of the genus Sphingomonas were also found: the strain was positive for catalase and b-galactosidase activities, hydrolysis of aesculin and assimilation of glucose, L-arabinose, maltose and DL-malate and was negative for arginine dihydrolase and urease activities and assimilation of D-mannitol and phenylacetate. However, some biochemical characteristics also differentiated strain A1-18 T biochemically from other related species (Table 1). Reverse-phase TLC of isoprenoid quinones extracted from A1-18 T showed a major spot corresponding to Q10, the characteristic respiratory quinone of the genus Sphingomonas (Yabuuchi et al., 2002). Cellular fatty acid composition was unique and distinguished strain A1-18 T from its phylogenetic neighbours. The presence of 2-hydroxymyristic acid (2-OH 14 : 0) and the absence of 3-hydroxy fatty acids in strain A1-18 T are consistent with other related species of the genus Sphingomonas (Table 2), but quantitative differences in the fatty acid composition distinguished A1-18 T from related species (Table 2). It is necessary to perform DNA DNA hybridization to judge whether a novel isolate belongs to the same species Table 1. Comparison of properties between Sphingomonas yabuuchiae sp. nov. A1-18 T and closely related Sphingomonas species Strains: 1, S. yabuuchiae sp. nov. A1-18 T ;2,S. paucimobilis ATCC T ;3,S. parapaucimobilis JCM 7510 T ;4,S. sanguinis IFO T ;5,S. trueperi ATCC T ;6,S. pituitosa DSM T ;7,S. roseiflava IAM T. W, Weakly positive reaction; DY, deep yellow; LY, light yellow; PY, pink yellow; ND, not done. Data were taken from this study, Yabuuchi et al. (1990), Kämpfer et al. (1997), Denner et al. (2001) and Yun et al. (2000). Characteristic Colour of colonies DY DY DY DY LY DY PY Oxidase Nitrate reduction Glucose acidification Hydrolysis of: Gelatin Starch Tween Assimilation of: D-Mannose N-Acetyl D-glucosamine W D-Gluconate W Caprate Adipate W W Sodium citrate W Utilization of: i-erythritol ND ND 2 Lactulose W ND ND 2 D-Psicose ND ND 2 L-Rhamnose ND ND 2 D-Sorbitol 2 W 2 2 ND ND 2 Turanose W ND ND 2 Formic acid W ND ND 2 D-Glucuronic 2 2 W + ND ND 2 p-hydroxyphenylacetic acid ND ND 2 Itaconic acid ND ND 2 Propionic acid ND ND 2 Succinamic acid W ND ND W D-Alanine ND ND 2 Glycerol ND ND 2 DNA G+C content (mol%) 66?1 63?7 64?7 62?1 65?6 64?5 67?
4 Y. Li and others Table 2. Fatty acid composition of selected Sphingomonas and Brevundimonas species Values are percentages of total fatty acids. Strains: 1, S. yabuuchiae sp. nov. A1-18 T ;2,S. paucimobilis ATCC T ;3,S. parapaucimobilis JCM 7510 T ;4,S. sanguinis IFO T ;5,S. trueperi ATCC T ;6,B. nasdae sp. nov. W1-2B T ;7,B. aurantiaca DSM 4731 T ;8,B. intermedia DSM 4732 T ;9,B. diminuta NCTC 8548 T ; 10, B. vesicularis ATCC T. ECL, Equivalent chain length; tr, trace (<1?0 %);, not detected. Data were from the present study, Abraham et al. (1999) and Denner et al. (2001). Summed features represent groups of two or three fatty acids that could not be separated by GLC with the MIDI system (Microbial ID). Summed feature 4 contained one or more of the following fatty acids: 16 : 1v7t, 15 : 0 iso 2-OH and 16 : 1v7c. Summed feature 7 contained one or more of the following fatty acids: 18 : 1v7c, 18:1v9t and/or 18 : 1v12t. In addition, B. nasdae contained 15 : 0 iso (1?54 %) and 17 : 0 iso (1?05 %). Fatty acid : 0 3-OH 3?1 2?3 1?7 1?5 1?9 12 : 1 3-OH tr tr tr 14 : 0 1?3 1?4 1?0 1?0 1?7 3?4 1?5 tr 2?4 14 : 0 2-OH 8?7 6?4 6?7 5?0 6?7 15 : 0 1?1 3?0 2?8 7?6 4?0 16 : 0 9?9 8?7 9?6 13?6 9?8 19?7 21?3 24?3 10?1 20?7 16 : 1v5c tr 1?9 1?0 Summed feature 4 5?9 2?7 8?6 6?7 2?5 4?0 7?5 1?0 5?1 17 : 0 1?2 0?5 1?6 3?8 1?7 1?6 8?5 2?4 17 : 1v6c 5?1 3?0 3?8 2?4 13?6 2?0 1?0 1?1 6?2 1?4 17 : 1v8c tr 2?6 1?6 2?0 10?8 2?6 ECL 17?897 1?3 tr 1?1 1?1 18 : 0 tr 0?6 1?0 tr tr tr tr Summed feature 7 62?5 74? ?6 64?2 51?6 56?5 49?4 38?7 53?7 18 : 1v5c 2?3 3?2 3?5 3?5 4?0 ECL 18?080 1?0 3?5 6?3 4?5 ECL 18?797 3?1 19 : 0 cyclo v8c 7?6 6?2 when they share more than 97 % 16S rdna sequence similarity (Stackebrandt & Goebel, 1994). Thus, the four species S. sanguinis, S. parapaucimobilis, S. paucimobilis and S. roseiflava were selected for DNA DNA hybridization. DNA DNA hybridization rates at optimal temperatures among these related species were only 46?4, 51?7, 40?7 and 24?7 % (Supplementary Table A in IJSEM Online). All hybridization rates were below the threshold value (approximately 70 %) under optimal hybridization conditions (T m 225 uc) that has been suggested as delineating a bacterial species (Grimont, 1999; Wayne et al., 1987). This confirmed that this strain represents a genetically independent species. In summary, morphological, biochemical, chemotaxonomic and phylogenetic study demonstrated that strain A1-18 T (=GTC 868 T ) represents a novel species within the genus Sphingomonas, for which we propose the name Sphingomonas yabuuchiae sp. nov. Strain W1-2B T Strain W1-2B T was isolated from condensed water. 16S rdna sequence analysis indicated that strain W1-2B T forms a distinct lineage within the genus Brevundimonas, forming a coherent cluster with B. vesicularis, B. aurantiaca and B. intermedia (Fig. 1). 16S sequence similarity was respectively 99?5, 98?8, 98?6 % and the similarities to other species of genus Brevundimonas were less than 97 % by CLUSTAL W analysis. The G+C content was 66?5 mol%, which is within the characteristic range of the genus Brevundimonas (65 68 mol%; Segers et al., 1994). These genetic results indicated that strain W1-2B T is affiliated with the genus Brevundimonas. Some similar phenotypic and biochemical characteristics to the Brevundimonas species also supported the assignment of strain W1-2B T as a member of the genus Brevundimonas. Cells were short, neatly arrayed rods arranged in a rosette pattern, Gram-negative, 1?5 4 mm long, 0?5 mm in diameter and motile in 0?3 % semi-solid agar. Colonies were slightly yellow, circular and smooth. No growth was observed at 4 uc. Like other species of genus Brevundimonas (Segers et al., 1994), strain W1-2B T is oxidase- and catalasepositive and the strain does not form indole, produce lipase (Tween 80 hydrolysis) or liquefy gelatin. However, some biochemical characteristics differentiate strain W1-2B T from closely related species of the genus Brevundimonas (Table 3). Examination of the reverse-phase, thin-layer chromatogram of isoprenoid quinones extracted from strain W1-2B T indicated that only ubiquinone was present. Strain W1-2B T produced a major spot corresponding to Q10, characteristic 822 International Journal of Systematic and Evolutionary Microbiology 54
5 Novel Sphingomonas and Brevundimonas species from Mir Table 3. Comparison of properties between Brevundimonas nasdae sp. nov. W1-2B T and related species of the genus Brevundimonas Strains: 1, B. nasdae sp. nov. W1-2B T ; 2, B. aurantiaca DSM 4731 T ; 3, B. intermedia DSM 4732 T ; 4, B. diminuta NCTC 8548 T ;5,B. vesicularis ATCC T. All strains were positive for catalase activity and negative for reduction of nitrate and nitrite, production of indole, acid from glucose and assimilation of L-arabinose, potassium gluconate, adipic acid, sodium citrate and phenyl acetate. W, Weakly positive. Characteristic Colour of colonies Slightly yellow Yellow Cream Slightly yellow Orange Hydrolysis of: Starch Aesculin Gelatin Utilization of: Galactose Fructose Sucrose W W L-Alanine L-Aspartic acid W L-Proline Succinate Arginine dihydrolase b-galactosidase Assimilation of: Glucose D-Mannose N-Acetyl D-glucosamine Maltose DL-Malic acid D-Mannitol 2 W 2 2 W n-capric acid Urease Oxidase of the genus Brevundimonas, members of which are aerobic with a respiratory type metabolism and Q10 is an intermediate electron carrier (Segers et al., 1994; Abraham et al., 1999). Cellular fatty acid analysis showed that summed feature 7 (one or more of 18 : 1v9t,18:1v7c and 18 : 1v12t; 51?6 %) and 16 : 0 (19?7 %) were the major fatty acids. This strain contained no 2-hydroxy acids, but did contain 3-hydroxy fatty acids (Table 2). These cellular fatty acid profiles are consistent with the description of the genus Brevundimonas: all strains are characterized by two major fatty acids, 16 : 0 and 18 : 1 (18 : 1v7c, 18: 1v9t and 18 : 1v12t) (Segers et al., 1994). Quantitative differences in the fatty acid composition, however, distinguished strain W1-2B T from the most closely related species B. vesicularis and B. intermedia (Table 2). The DNA DNA hybridization data showed that strain W1-2B T had relatedness values of only 51?6, 54?8 and 50?2 %, respectively, with its closely related neighbours, B. vesicularis, B. aurantiaca and B. intermedia (Supplementary Table B in IJSEM Online). All hybridization rates were below the threshold value (approximately 70 %) that has been suggested as delineating a bacterial species (Wayne et al., 1987). The quantitative hybridization results indicate that this strain genetically represents an independent species. In summary, the characteristics of strain W1-2B T are consistent with the description of genus Brevundimonas with regard to morphology, biochemical and chemotaxonomic properties. They also support the conclusion from 16S rdna alignment that strain W1-2B T belongs to the genus Brevundimonas. However, some biochemical reactions and cellular fatty acid composition differentiate it from existing species of the genus. DNA DNA hybridization further confirmed that strain W1-2B T taxonomically represents an independent species. On the basis of these results, strain W1-2B T is proposed as the type strain of a novel species of the genus Brevundimonas, Brevundimonas nasdae sp. nov
6 Y. Li and others Description of Sphingomonas yabuuchiae sp. nov. Sphingomonas yabuuchiae (ya.bu.u9chi.ae. N.L. gen. n. yabuuchiae of Yabuuchi, in honour of Eiko Yabuuchi, a Japanese bacteriologist, who proposed the genus name Sphingomonas). Grows well under aerobic conditions at 30 uc on BHI agar plates. Gram-negative, has rod-shaped cells with rounded ends, 1 4 mm long and 0?5 mm in diameter. Colonies are deep yellow, circular and smooth. Hydrolyses glycerol, starch and Tween 80 but not gelatin or ornithine. Does not reduce nitrate. Utilizes L-arabinose, cellobiose, fructose, galactose, glucose, lactose, maltose, mannose, melibiose, raffinose, sucrose and trehalose but not adonitol, inositol, mannitol, rhamnose or sorbitol. Catalase activity is positive. Q10 is the major isoprenoid quinone. 18 : 1 is the major cellular fatty acid and the major 2-hydroxy acid is 2-OH 14 : 0, while 3-hydroxy fatty acids are absent. The G+C content is 66?1 mol%. The type strain, A1-18 T (=GTC 868 T =JCM T =DSM T ), was isolated from an air sample from the Russian space station Mir. Description of Brevundimonas nasdae sp. nov. Brevundimonas nasdae (nas9dae. N.L. gen. n. nasdae of NASDA, arbitrary name referring to the National Space Development Agency of Japan, which contributed to this project). Grows well under aerobic conditions at 30 uc on BHI agar plates. Gram-negative, has short rod-shaped cells and produces creamy white, circular and smooth colonies. No growth at 4 uc or in media containing 4 % NaCl. Positive for oxidase and catalase activity and negative for reduction of nitrate and nitrite, production of phenylalanine deaminase, urease, indole and acylamidase, hydrolysis of DNA, starch and arginine, decarboxylation of lysine and acid from mannitol. Utilizes acetate, pyruvate, methyl pyruvate, succinate and amino acids, including L-alanine, L-aspartic acid, glutamate and L-proline, but not arabinose, mannose, fructose or lactose. Produces acid from glucose, galactose, maltose and sucrose but is not able to hydrolyse Tween 80 or glycerol. Q10 is the major isoprenoid quinone. 18 : 1 (51?6 %) and 16 : 0 (19?7%) are the most abundant cellular fatty acids and the G+C content is 66?5 mol%. The type strain, W1-2B T (=GTC 1043 T =JCM T =DSM T ), was isolated from a condensation water sample from the Russian space station Mir. ACKNOWLEDGEMENTS This work was supported by the first Japanese and Russian collaboration on the Mir Utilization Project and a grant from the Japan Space Utilization Promotion Center (JSUP). REFERENCES Abraham, W.-R., Strömpl, C., Meyer, H. & 8 other authors (1999). Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. Int J Syst Bacteriol 49, Denner, E. B. M., Paukner, S., Kämpfer, P., Moore, E. R. B., Abraham, W.-R., Busse, H.-J., Wanner, G. & Lubitz, W. (2001). Sphingomonas pituitosa sp. nov., an exopolysaccharide-producing bacterium that secrets an unusual type of sphingan. Int J Syst Evol Microbiol 51, Ezaki, T., Hashimoto, Y. & Yabuuchi, E. (1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, Ezaki, T., Saidi, S. M., Liu, S. L., Hashimoto, Y., Yamamoto, H. & Yabuuchi, E. (1990). Rapid procedure to determine the DNA base composition from small amounts of Gram-positive bacteria. FEMS Microbiol Lett 55, Ezaki, T., Li, N., Hashimoto, Y., Miura, H. & Yamamoto, H. (1994). 16S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov. Int J Syst Bacteriol 44, Garrity, G. M. & Holt, J. G. (2001). The road map to the Manual. In Bergey s Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer. Grimont, P. A. D. (1999). Taxonomy and classification of bacteria. In Manual of Clinical Microbiology, 7th edn, pp Edited by P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover & R. H. Yolken. Washington, DC: American Society for Microbiology. Kämpfer, P. & Kroppenstedt, R. M. (1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, Kämpfer, P., Denner, E. B. M., Meyer, S., Moore, E. R. B. & Busse, H.-J. (1997). Classification of Pseudomonas azotocolligans Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47, Kawamura, Y., Li, Y., Liu, H., Huang, X., Li, Z. & Ezaki, T. (2001). Bacterial population in Russian space station Mir. Microbiol Immunol 45, Page, R. D. M. (1996). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, Pearson, W. R. & Lipman, D. J. (1988). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, Saitou, N. & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, Segers, P., Vancanneyt, M., Pot, B., Torck, U., Hoste, B., Dewettinck, D., Falsen, E., Kersters, K. & De Vos, P. (1994). Classification of Pseudomonas diminuta Leifson and Hugh 1954 and Pseudomonas vesicularis Büsing, Döll, and Freytag 1953 in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb. nov., respectively. Int J Syst Bacteriol 44, Stackebrandt, E. & Goebel, B. M. (1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rrna sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, International Journal of Systematic and Evolutionary Microbiology 54
7 Novel Sphingomonas and Brevundimonas species from Mir Thompson, J. D., Higgins, D. G. & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequencing weighing, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors (1987). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, Yabuuchi, E., Yano, I., Oyaizu, H., Hashimoto, Y., Ezaki, T. & Yamamoto, H. (1990). Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas. Microbiol Immunol 34, Yabuuchi, E., Kosako, Y., Fujiwara, N., Naka, T., Matsunaga, I., Ogura, H. & Kobayashi, K. (2002). Emendation of the genus Sphingomonas Yabuuchi et al and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola. Int J Syst Evol Microbiol 52, Yano, I., Yamada, Y. & Suzuki, K. I. (1987). Analysis of bacterial ingredients. In Bacterial Identification in Accordance with Recent Taxonomy: Use of Phenotypic, Chemical, and Genetic Analysis, pp Edited by E. Yabuuchi, T. Ezaki, Y. H. Park, H. Sugawara, K. I. Suzuki, Y. Yamada, H. Yamanoto & I. Yano. Tokyo: Saikon Publications. Yun, N. R., Shin, Y. K., Hwang, S. Y., Kuraishi, H., Sugiyama, J. & Kawahara, K. (2000). Chemotaxonomic and phylogenetic analyses of Sphingomonas strains isolated from ears of plants in the family Gramineae and a proposal of Sphingomonas roseoflava sp. nov. J Gen Appl Microbiol 46,
16S rdna-based phylogenetic analysis. The first 443 bp of the 16S rrna gene were
1 Supporting Information 2 16S rdna-based phylogenetic analysis. The first 443 bp of the 16S rrna gene were 3 briefly amplified from the bacterial genomic DNA by PCR using a PCR Master Mix including 4
More informationPseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria
International Journal of Systematic and Evolutionary Microbiology (2010), 60, 1177 1181 DOI 10.1099/ijs.0.015073-0 Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description
More informationHumibacter antri sp. nov., an actinobacterium isolated from a natural cave, and emended description of the genus Humibacter
International Journal of Systematic and Evolutionary Microbiology (2013), 63, 4315 4319 DOI 10.1099/ijs.0.050708-0 Humibacter antri sp. nov., an actinobacterium isolated from a natural cave, and emended
More informationDevosia insulae sp. nov., isolated from soil, and emended description of the genus Devosia
International Journal of Systematic and Evolutionary Microbiology (2007), 57, 1310 1314 DOI 10.1099/ijs.0.65028-0 Devosia insulae sp. nov., isolated from soil, and emended description of the genus Devosia
More informationMOTILE ENTEROCOCCI (STREPTOCOCCUS FAECIUM VAR. MOBILIS VAR. N.) ISOLATED FROM GRASS SILAGE
MOTILE ENTEROCOCCI (STREPTOCOCCUS FAECIUM VAR. MOBILIS VAR. N.) ISOLATED FROM GRASS SILAGE C. W. LANGSTON, JOYCE GUTIERREZ, AND CECELIA BOUMA Dairy Cattle Research Branch, Agricultural Research Center,
More informationTable 1: Colony morphology and cultural characteristics of isolated strains after incubation at 28 o C for 72 h.
Table 1: Colony morphology and cultural characteristics of isolated strains after incubation at 28 o C for 72 h. Bacterial Media used Colony morphology strains VR1 YEMA Small (2 mm), opaque, circular,
More information202 S. IsExi and T. IKEDA [Vol. 32,
No. 3] 201 47. On Bacterial Enzyme Specifically Decomposing Group B Substance By Shoei ISEKI and Tsukasa IKEDA Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan (Comm.
More informationPrevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces
International Journal of Systematic and Evolutionary Microbiology (2007), 57, 941 946 DOI 10.1099/ijs.0.64778-0 Prevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces Hidenori
More informationSphingomonas aestuarii sp. nov., isolated from tidal flat sediment
International Journal of Systematic and Evolutionary Microbiology (2009), 59, 1359 1363 DOI 10.1099/ijs.0.65528-0 Sphingomonas aestuarii sp. nov., isolated from tidal flat sediment Seong Woon Roh, 1 Kyoung-Ho
More informationPseudomonas psychrotolerans sp. nov.
International Journal of Systematic and Evolutionary Microbiology (2004), 54, 1633 1637 DOI 10.1099/ijs.0.03024-0 Pseudomonas psychrotolerans sp. nov. Elke Hauser, 1 Peter Kämpfer 2 and Hans-Jürgen Busse
More informationA report of 14 unrecorded bacterial species in Korea isolated in 2017
Journal of Species Research 7(2):161-180, 2018 A report of 14 unrecorded bacterial species in Korea isolated in 2017 Ju-Young Kim 1, Jun Hwee Jang 1, Soohyun Maeng 2, Myung-Suk Kang 3 and Myung Kyum Kim
More informationStrain DSM Genus. alimentaria Status Risk group Type strain 72, JCM 16360, KACC Reference Author
Strain DSM 45698 Genus Dietzia Species alimentaria Status Risk group L1 Type strain 72, JCM 16360, KACC 21126 Reference Author Title Journal Kim, J., Roh, S. W., Choi, J. H., Jung, M. J., Nam, Y. D., Kim,
More informationPsychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood
International Journal of Systematic and Evolutionary Microbiology (2003), 53, 449 454 DOI 10.1099/ijs.0.02242-0 Note Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented
More informationEiko Yabuuchi, 1 Yoshimasa Kosako, 2 Nagatoshi Fujiwara, 3 Takashi Naka, 3,4 Isamu Matsunaga, 5 Hisashi Ogura 5 and Kazuo Kobayashi 3
International Journal of Systematic and Evolutionary Microbiology (2002), 52, 1485 1496 DOI: 10.1099/ijs.0.01868-0 Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy
More informationPseudidiomarina donghaiensis sp. nov. and Pseudidiomarina maritima sp. nov., isolated from the East China Sea
International Journal of Systematic and Evolutionary Microbiology (2009), 59, 1321 1325 DOI 10.1099/ijs.0.005702-0 Pseudidiomarina donghaiensis sp. nov. and Pseudidiomarina maritima sp. nov., isolated
More informationKistimonas scapharcae sp. nov., isolated from a dead ark clam (Scapharca broughtonii), and emended description of the genus Kistimonas
International Journal of Systematic and Evolutionary Microbiology (2012), 62, 2865 2869 DOI 10.1099/ijs.0.038422-0 Kistimonas scapharcae sp. nov., isolated from a dead ark clam (Scapharca broughtonii),
More informationSphingopyxis soli sp. nov., isolated from landfill soil
International Journal of Systematic and Evolutionary Microbiology (2010), 60, 1682 1686 DOI 10.1099/ijs.0.013128-0 Sphingopyxis soli sp. nov., isolated from landfill soil Jung-Hye Choi, 1 Min-Soo Kim,
More informationSphingomonas hengshuiensis sp. nov., isolated from lake wetland
International Journal of Systematic and Evolutionary Microbiology (2015), 65, 4644 4649 DOI 10.1099/ijsem.0.000626 Sphingomonas hengshuiensis sp. nov., isolated from lake wetland Shuzhen Wei, 1 Tingting
More informationstaphylococci. They found that of 28 strains of staphylococci from foods STAPHYLOCOCCI AND RELATED VARIETIES
A COMPARATIVE STUDY OF KNOWVN FOOD-POISONING STAPHYLOCOCCI AND RELATED VARIETIES JAMES B. EVANS AND C. F. NIVEN, JR. Division of Bacteriology, American Meat Institute Foundation, and the Department of
More informationOVERVIEW OF CURRENT IDENTIFICATION SYSTEMS AND DATABASES
OVERVIEW OF CURRENT IDENTIFICATION SYSTEMS AND DATABASES EVERY STEP OF THE WAY 1 EVERY STEP OF THE WAY MICROBIAL IDENTIFICATION METHODS DNA RNA Genotypic Sequencing of ribosomal RNA regions of bacteria
More informationPseudonocardia kongjuensis sp. nov., isolated from a gold mine cave
International Journal of Systematic and Evolutionary Microbiology (2001), 51, 1505 1510 Printed in Great Britain Pseudonocardia kongjuensis sp. nov., isolated from a gold mine cave 1 School of Biological
More informationScholars Research Library. Purification and characterization of neutral protease enzyme from Bacillus Subtilis
Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res., 2012, 2 (4):612-618 (http://scholarsresearchlibrary.com/archive.html) Purification and characterization
More informationIsolation and Biochemical Characterization of Lactobacillus species Isolated from Dahi
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 4 (2016) pp. 1042-1049 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.504.119
More informationUreibacillus defluvii sp. nov., isolated from a thermophilic microbial fuel cell
International Journal of Systematic and Evolutionary Microbiology (2014), 64, 1617 1621 DOI 10.1099/ijs.0.056655-0 Ureibacillus defluvii sp. nov., isolated from a thermophilic microbial fuel cell Shungui
More informationIdentification of Unknown Indigenous Bacteria
April 29, 2009 Identification of Unknown Indigenous Bacteria Introduction Many bacteria can be found in and on nearly all areas of the healthy human body. These bacteria are referred to as normal flora
More informationAPI TEST OF LACTOBACILLI ISOLATED FROM TOP BRANDS COMMERCIAL YOGURT. Satchanska, G. and D. Illin
API TEST OF LACTOBACILLI ISOLATED FROM TOP BRANDS COMMERCIAL YOGURT Satchanska, G. and D. Illin I N T R O D U C T I O N Yogurt possess numerous health beneficial effects, incl. detoxification capability.
More informationSphingomonas gei sp. nov., isolated from roots of Geum aleppicum
International Journal of Systematic and Evolutionary Microbiology (2015), 65, 1160 1166 DOI 10.1099/ijs.0.000074 Sphingomonas gei sp. nov., isolated from roots of Geum aleppicum Lingfang Zhu,3 Meiru Si,3
More informationChryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., isolated from roots of sand-dune plants
International Journal of Systematic and Evolutionary Microbiology (2006), 56, 433 438 DOI 10.1099/ijs.0.63825-0 Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., isolated
More informationS. aureus NCTC 6571, E. coli NCTC (antibiotic
ISO Sensitivity Test Agar Code: KM1204 A semi-defined nutritionally rich sensitivity medium. It is composed of specially selected peptones with a small amount of glucose, solidified with a very pure agar
More informationDuganella ginsengisoli sp. nov., isolated from ginseng soil
International Journal of Systematic and Evolutionary Microbiology (2016), 66, 56 61 DOI 10.1099/ijsem.0.000669 Duganella ginsengisoli sp. nov., isolated from ginseng soil Jinglou Zhang, 1 3 Yeon-Ju Kim,3
More informationYUZO YAMADA AND MANAMI AKITA. Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Shizuoka University, Shizuoka 422, Japan
J. Gen. App!. Microbiol., 30,115-126 (1984) AN ELECTROPHORETIC COMPARISON IN STRAINS OF GL UCONOBACTER OF SPECIES' ENZYMES YUZO YAMADA AND MANAMI AKITA Laboratory of Applied Microbiology, Department of
More informationNov., and Emendation of the Genus Sphingomonas. Eiko Yabuuchi*,', Yoshimasa Kosako2, Takashi Naka3, Shinnichiro Suzuki4, and Ikuya Yano3
Microbiol. Immunol., 43(4), 339-349, 1999 Proposal of Sphingomonas suberifaciens (van Bruggen, Jochimsen and Brown 1990) Comb. Nov., Sphingomonas natatoria (Sly 1985) Comb. Nov., Sphingomonas ursincola
More information6/28/2016. Growth Media and Metabolism. Complex Media. Defined Media. Made from complex and rich ingredients
Growth Media and Metabolism Complex Media Made from complex and rich ingredients Ex. Soya protein extracts Milk protein extracts Blood products Tomato juice, etc. Exact chemical composition unknown Can
More informationSphingopyxis ummariensis sp. nov., isolated from a hexachlorocyclohexane dump site
International Journal of Systematic and Evolutionary Microbiology (2010), 60, 780 784 DOI 10.1099/ijs.0.008805-0 Sphingopyxis ummariensis sp. nov., isolated from a hexachlorocyclohexane dump site Pooja
More informationInternational Journal of Systematic and Evolutionary Microbiology (2010), 60,
International Journal of Systematic and Evolutionary Microbiology (2010), 60, 963 971 DOI 10.1099/ijs.0.014480-0 Taxonomic study of the genus Salinicola: transfer of Halomonas salaria and Chromohalobacter
More information1~~~~~~~~~~~~~~~~~~~~~~~~~~
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Nov. 1985, p. 1213-1218 0099-2240/85/111213-06$02.00/0 Copyright C) 1985, American Society for Microbiology Vol. 50, No. 5 Characterization of Dysgonic, Heterotrophic
More informationcepacia (Palleroni and Holmes 1981) comb. nov.
Microbiol. Immunol. Vol. 36 (12), 1251-1275, 1992 Proposal of Burkholderi a gen. nov. and Transfer of Seven Species of the Genus Pseudomonas Homology Group II to the New Genus, with the Type Species Burkholderia
More informationMicrobiology Activity #6 Metabolism of Small Molecules.
Microbiology Activity #6 Metabolism of Small Molecules. Analysis of Carbohydrate Metabolism Organisms that use CO 2 as a carbon source and fix the carbon into biomass are autotrophs, usually obtaining
More informationPRESENTER: DENNIS NYACHAE MOSE KENYATTA UNIVERSITY
18/8/2016 SOURCES OF MICROBIAL CONTAMINANTS IN BIOSAFETY LABORATORIES IN KENYA PRESENTER: DENNIS NYACHAE MOSE KENYATTA UNIVERSITY 1 INTRODUCTION Contamination occurs through avoidable procedural errors
More informationDietzia kunjamensis sp. nov., isolated from the Indian Himalayas
International Journal of Systematic and Evolutionary Microbiology (2006), 56, 1667 1671 DOI 10.1099/ijs.0.64212-0 Dietzia kunjamensis sp. nov., isolated from the Indian Himalayas S. Mayilraj, 1 K. Suresh,
More informationSalipiger nanhaiensis sp. nov., a bacterium isolated from deep sea water
International Journal of Systematic and Evolutionary Microbiology (2015), 65, 1122 1126 DOI 10.1099/ijs.0.000066 Salipiger nanhaiensis sp. nov., a bacterium isolated from deep sea water Xiaofeng Dai, Xiaochong
More informationPontibaca methylaminivorans gen. nov., sp. nov., a member of the family Rhodobacteraceae
International Journal of Systematic and Evolutionary Microbiology (2010), 60, 2170 2175 DOI 10.1099/ijs.0.020172-0 Pontibaca methylaminivorans gen. nov., sp. nov., a member of the family Rhodobacteraceae
More informationLab #9. Introduction. Class samples:
Lab #9 Introduction Food-borne illness is largely caused by the presence of bacteria in red meat. However, much of these harmful bacteria can be destroyed and prevented by sanitation and safe cooking practices.
More informationEvaluation of the Enteric-Tek System for Identifying Enterobacteriaceae
JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1982, p. 419-424 Vol. 15, No. 3 0095-1137/82/030419-06$02.00/0 Evaluation of the Enteric-Tek System for Identifying Enterobacteriaceae A. 0. ESAIAS,* D. L. RHODEN,
More informationPartial characterization and optimization of alkaline Amaylase from Bacillus spp. from Lonar Crater
INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACY, BIOLOGY AND CHEMISTRY Research Article Partial characterization and optimization of alkaline Amaylase from Bacillus spp. from Lonar Crater Tambekar DH, Chandurkar
More informationAcidomonas gen. nov. Incorporating Acetobacter methanolicus as Acidomonas methanolica comb. nov.
~NTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, Jan. 1989, p. 5-55 2-7713/89/15-6$2.OO/O Copyright 1989, International Union of Microbiological Societies Vol. 39. No. 1 Acidomonas gen. nov. Incorporating
More informationSTUDIES ON THE ASAKUSA GROUP OF ENTEROBACTERIACEAE (EDWARDSIELLA TARDA)
Japan. J. Med. Sci. Biol., 20, 205-212, 1967 STUDIES ON THE ASAKUSA GROUP OF ENTEROBACTERIACEAE (EDWARDSIELLA TARDA) RIICHI SAKAZAKI Department of Bacteriology I, National Institute of Health, Tokyo (Received:
More informationSHIGELLA. Bacillary dysentery is caused by genus Shigella, named after Shiga who isolated them.
24 SHIGELLA 24.1 INTRODUCTION Bacillary dysentery is caused by genus Shigella, named after Shiga who isolated them. OBJECTIVES After reading this lesson, you will be able to: describe the characteristics
More informationSCREENING OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)
Chapter 4 Results 4. RESULTS SCREENING OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) Totally 92 wound samples were collected from the major sites of coastal area such as Cuddalore, Pondicherry,
More informationCitrobacter koseri. II. Serological and biochemical examination of Citrobacter koseri strains from clinical specimens
J. Hyg., Camb. (1975), 75, 129 129 Printed in Great Britain Citrobacter koseri. II. Serological and biochemical examination of Citrobacter koseri strains from clinical specimens BY B. ROWE, R. J. GROSS
More informationTwo New Species of Pseudomonas: P. oryzihabitans Isolated from Rice Paddy and Clinical Specimens and P. luteola Isolated from Clinical Specimens
INERNAIONAL JOURNAL OF SYSEMAIC BACERIOLOGY, Oct. 1985, p. 467-474 0020-7713/85/040467-08$02.00/0 Copyright 0 1985, International Union of Microbiological Societies Vol. 35, No. 4 wo New Species of Pseudomonas:
More informationIMMUNOLOGICAL AND BACTERIOLOGICAL STUDIES ON ORNITHOBACTERIUM RHINOTRACHEALIS
IMMUNOLOGICAL AND BACTERIOLOGICAL STUDIES ON ORNITHOBACTERIUM RHINOTRACHEALIS PRESENTED BY RABAB AMIN KHALIFA UNDER THE SUPERVISION OF Prof. Dr. Mohamed Refai Prof. of Microbiology, Faculty of Vet. Med.
More informationSelective Growth Media for Differentiation and Detection of Escherichia Coli and Other Coliforms
Page 1 of 5 Page 1 of 5 Return to Web Version Selective Growth Media for Differentiation and Detection of Escherichia Coli and Other Coliforms By: Jvo Siegrist, AnalytiX Volume 8 Article 4 E. coli and
More informationUnderstanding Gallibacterium-Associated Peritonitis in the Commercial Egg-Laying Industry
Understanding Gallibacterium-Associated Peritonitis in the Commercial Egg-Laying Industry Timothy J. Johnson A, Lisa K. Nolan B, and Darrell W. Trampel C A University of Minnesota, Department of Veterinary
More informationCHARACTERISTICS OF RUMINAL ANAEROBIC CELLULOLYTIC
CHARACTERISTICS OF RUMINAL ANAEROBIC CELLULOLYTIC COCCI AND CILLOBACTERIUM CELLULOSOLVENS N. SP. M. P. BRYANT, NOLA SMALL,' CECELIA BOUMA, AND I. M. ROBINSON Dairy Cattle Research Branch, U. S. Department
More informationInternational Journal of Systematic and Evolutionary Microbiology (2009), 59, Miyahara, Fujieda, Shizuoka , Japan
International Journal of Systematic and Evolutionary Microbiology (2009), 59, 1748 1753 DOI 10.1099/ijs.0.007674-0 Butyricimonas synergistica gen. nov., sp. nov. and Butyricimonas virosa sp. nov., butyric
More informationCroceitalea litorea sp. nov., isolated from seashore sand
International Journal of Systematic and Evolutionary Microbiology (2015), 65, 4563 4567 DOI 10.1099/ijsem.0.000613 Croceitalea litorea sp. nov., isolated from seashore sand Minwook Kim, 1 3 In-Tae Cha,
More informationPromicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval Virgilkapelle in Vienna
International Journal of Systematic and Evolutionary Microbiology (2003), 53, 1503 1507 DOI 10.1099/ijs.0.02522-0 Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated
More informationDNA DNA Reassociation Methods Applied to Microbial Taxonomy and Their Critical Evaluation
2 DNA DNA Reassociation Methods Applied to Microbial Taxonomy and Their Critical Evaluation Ramon Rosselló-Mora 2.1 Introduction DNA DNA reassociation techniques are used for many purposes, but in the
More informationNew genomic typing method MLST
New genomic typing method MLST Bon KIMURA fingerprinting PFGE DNA multilocus sequence typingmlst alleles PFGE MLST 1990 PCR 1 PCR DNA PFGE 1 PFGE RAPDrandomly amplified polymorphic DNA 3 AFLPAmplified
More informationSections 11 & 12: Isolation and Identification of Enterobacteriaceae
Sections 11 & 12: Isolation and Identification of Enterobacteriaceae The family Enterobacteriaceae includes many genera and species. The last edition of Bergey s Manual of Systematic Bacteriology (Vol.
More informationActinomyces marimammalium sp. nov., from marine mammals
International Journal of Systematic and Evolutionary Microbiology (2001), 51, 151 156 Printed in Great Britain Actinomyces marimammalium sp. nov., from marine mammals Lesley Hoyles, 1 Cristina Pascual,
More informationBiochemical Testing Handout
Biochemical Testing Handout As you guys know, the purpose of a medical microbiology laboratory is to mainly isolate and identify organisms to provide proper treatment. For this week we will focus on five
More informationorganisms isolated from fermenting substances no characters PLANTARUM (ORLA-JENSEN) BERGEY
A STUDY OF THE SPECIES LACTOBACILLUS PLANTARUM (ORLA-JENSEN) BERGEY ET AL.1 CARL S. PEDERSON2 New York State Agricultural Experiment Station, Geneva, New York Received for publication, November 5, 1935
More informationGram-negative rods. Enterobacteriaceae. Biochemical Reactions. Manal AL khulaifi
Gram-negative rods Enterobacteriaceae Biochemical Reactions Bacteria Gram positive Gram negative Cocci Bacilli Cocci Rods Characters of Enterobacteriaceae All Enterobacteriaciae Gram-negative rods Reduce
More informationTween 40-based precipitate production observed on modified chromogenic agar and development of biological identification kit for Malassezia species
Medical Mycology May 2006, 44, 227231 Tween 40-based precipitate production observed on modified chromogenic agar and development of biological identification kit for Malassezia species TAKAMASA KANEKO*$,
More informationPhases Available Description Applications Additional Notes RCM-Monosaccharide (L19 packing)*
Carbohydrate and Organic Acid Analysis Excellent resolution Wide range of selectivities Excellent column-to-column reproducibility Recommended alternative to Bio-Rad, Supelco Supelcogel and Waters Sugar-Pak
More informationInternational Journal of Systematic and Evolutionary Microbiology (2015), 65, Tomohiro Watanabe, Hisaya Kojima and Manabu Fukui
International Journal of Systematic and Evolutionary Microbiology (2015), 65, 1504 1508 DOI 10.1099/ijs.0.000129 Sulfuriferula multivorans gen. nov., sp. nov., isolated from a freshwater lake, reclassification
More informationSeparation and analysis of free ceramides containing 2-hydroxy fatty acids in Sphingobacterium species
FEMS Microbiology Letters 20 (1983) 449-453 449 Published by Elsevier Separation and analysis of free ceramides containing 2-hydroxy fatty acids in Sphingobacterium species (Bacterial sphingolipid; ceramide;
More informationCarboxylicivirga linearis sp. nov., isolated from a sea cucumber culture pond
International Journal of Systematic and Evolutionary Microbiology (2015), 65, 3271 3275 DOI 10.10/ijsem.0.000407 Carboxylicivirga linearis sp. nov., isolated from a sea cucumber culture pond Feng-Qing
More informationA report of 28 unrecorded bacterial species, phylum Bacteroidetes, in Korea
104 Journal of Species Research 7(2):104-113, 2018 JOURNAL OF SPECIES RESEARCH Vol. 7, No. 2 A report of 28 unrecorded bacterial species, phylum Bacteroidetes, in Korea Soohyun Maeng 1, Chaeyun Baek 1,
More informationSphingomonas zeae sp. nov., isolated from the stem of Zea mays
International Journal of Systematic and Evolutionary Microbiology (2015), 65, 2542 2548 DOI 10.1099/ijs.0.000298 Sphingomonas zeae sp. nov., isolated from the stem of Zea mays Peter Kämpfer, 1 Hans-Jürgen
More informationNosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface of bottles of saline solution used in wound cleansing
International Journal of Systematic and Evolutionary Microbiology (2008), 58, 2939 2944 DOI 10.1099/ijs.0.65753-0 Nosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface of bottles of saline
More informationMedical Microbiology
Lecture 5!!!!!!ƒš!!Œ!!! š!!œ!! Œ!!!! Dr. Ismail I. Daood Medical Microbiology!! Systematic Bacteriology Gram-Positive Cocci : GENUS : Staphylococcus : The general properties of Staphylococcus are Gram-
More informationFirst Clinical Isolates of Nocardia carnea, Nocardia elegans, Nocardia paucivorans, Nocardia puris and Nocardia takedensis in Japan
Jpn. J. Med. Mycol. Vol. 47, 85 89, 2006 ISSN 0916 4804 Original Article First Clinical Isolates of Nocardia carnea, Nocardia elegans, Nocardia paucivorans, Nocardia puris and Nocardia takedensis in Japan
More informationMalassezia, 2. Malassezia
Jpn. J. Med. Mycol. Vol. 46, 163 167, 2005 ISSN 0916 4804 Malassezia Malassezia,,, SD, AD, Malassezia. SD, AD Malassezia PCR. AD, SD M. globosa, M. restricta,. AD 20, 2, Malassezia., 2 Malassezia 90, 70.
More informationUndibacterium aquatile sp. nov., isolated from a waterfall
International Journal of Systematic and Evolutionary Microbiology (2015), 65, 4128 4133 DOI 10.1099/ijsem.0.000545 Undibacterium aquatile sp. nov., isolated from a fall Juan Du, 1 3 Shahina Akter, 1 3
More informationInternational Journal of Systematic and Evolutionary Microbiology (2009), 59,
International Journal of Systematic and Evolutionary Microbiology (2009), 59, 509 516 DOI 10.1099/ijs.0.000950-0 Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov.
More information6 The chemistry of living organisms
Living organisms are composed of about 22 different chemical elements. These are combined to form a great variety of compounds. Six major elements make up almost 99% of the mass of the human body, as shown
More informationInternational Journal of Systematic and Evolutionary Microbiology (2013), 63,
International Journal of Systematic and Evolutionary Microbiology (2013), 63, 815 820 DOI 10.1099/ijs.0.030155-0 Sphingobacterium hotanense sp. nov., isolated from soil of a Populus euphratica forest,
More informationTAXONOMIC STUDIES ON CORYNEFORM BACTERIA IV. MORPHOLOGICAL, CULTURAL, BIOCHEMICAL, AND PHYSIOLOGICAL CHARACTERISTICS
J. Gen. Appl. iylicrobiol., 18, 399-416 (1972) TAXONOMIC STUDIES ON CORYNEFORM BACTERIA IV. MORPHOLOGICAL, CULTURAL, BIOCHEMICAL, AND PHYSIOLOGICAL CHARACTERISTICS KAZUHIKO YAMADA AND KAZUO KOMAGATA' Central
More informationMucilaginibacter carri sp. nov., isolated from a car air conditioning system
International Journal of Systematic and Evolutionary Microbiology (2016), 66, 1754 1759 DOI 10.1099/ijsem.0.000938 Mucilaginibacter carri sp. nov., isolated from a car air conditioning system Dong-Uk Kim,
More informationBifidobacterium Species Expressing Phenotypical Similarity to Bifidobacterium adolescentis Isolated from
Full Paper Bifidobacteria Microflora Vol. 11 (1), 25-32, 1992 Bifidobacterium Species Expressing Phenotypical Similarity to Bifidobacterium adolescentis Isolated from the Feces of Human Adults Tomoko YAESHIMA,1
More informationE.coli Core Model: Metabolic Core
1 E.coli Core Model: Metabolic Core 2 LEARNING OBJECTIVES Each student should be able to: Describe the glycolysis pathway in the core model. Describe the TCA cycle in the core model. Explain gluconeogenesis.
More informationMetabolic response induced by parasitic plant-fungus interactions hinder amino sugar and nucleotide sugar metabolism in the host
Supplementary information Metabolic response induced by parasitic plant-fungus interactions hinder amino sugar and nucleotide sugar metabolism in the host Dong-Kyu Lee, Soohyun Ahn, Hae Yoon Cho, Hye Young
More informationStudent Perspectives on the Use of Biolog GenIII Plates in Undergraduate Research and a General Microbiology Course
Student Perspectives on the Use of Biolog GenIII Plates in Undergraduate Research and a General Microbiology Course Jordan Krebs & Jeff Newman Lycoming College Williamsport, PA Talk Contents The use of
More informationGuido Funke* and Reinhard Frodl. Department of Medical Microbiology and Hygiene, Gärtner & Colleagues Laboratories, Ravensburg, Germany
JOURNAL OF CLINICAL MICROBIOLOGY, Feb. 2008, p. 638 643 Vol. 46, No. 2 0095-1137/08/$08.00 0 doi:10.1128/jcm.01491-07 Copyright 2008, American Society for Microbiology. All Rights Reserved. Comprehensive
More informationThe Characteristics of Lactobacillus plantarum, L. helveticus and L. casei
133 WHEATER, D. M. (1955). J. gen. Microbial. 12, 133-139. The Characteristics of Lactobacillus plantarum, L. helveticus and L. casei BY DOROTHY M. WHEATER* National Institute for Research in Dairying,
More informationJ. Environ. Res. Develop. Journal of Environmental Research And Development Vol. 8 No. 2, October-December 2013
LIPOLYTIC PSYCHROTROPHIC GRAM POSITIVE IN MILK AND FERMENTED MILK PRODUCTS Patil Sunita Hanamant* 1 and Gandhi Mohanlal Bansilal 2 1. Department of Microbiology, K.T.H.M. College, Nashik, Maharashtra (INDIA)
More informationPaenibacillus marinisediminis sp. nov., a Bacterium Isolated from Marine Sediment
Journal of Microbiology (2013) Vol. 51, No. 3, pp. 312 317 Copyright c 2013, The Microbiological Society of Korea DOI 10.1007/s12275-013-3198-2 Paenibacillus marinisediminis sp. nov., a Bacterium Isolated
More informationSaccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera)
International Journal of Systematic and Evolutionary Microbiology (2008), 58, 628 632 DOI 10.1099/ijs.0.64971-0 Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge
More informationID of Most Common Bacterial Pathogens. CLS 417- Clinical Practice in Microbiology Miss Zeina Alkudmani
ID of Most Common Bacterial Pathogens CLS 417- Clinical Practice in Microbiology Miss Zeina Alkudmani BACTERIA Gram Positive Gram Negative Cocci Bacilli Bacilli Cocci Coccobacilli - Staph - Strept - Clostridium
More informationMetabolomics approach reveals metabolic disorders and potential. biomarkers associated with the developmental toxicity of
Supplementary information for Metabolomics approach reveals metabolic disorders and potential biomarkers associated with the developmental toxicity of tetrabromobisphenol A and tetrachlorobisphenol A Guozhu
More informationEXERCISE. Proteins,Amino Acids, and Enzymes VII: Oxidase Test. Suggested Reading in Textbook. Pronunciation Guide. Materials per Student
EXERCISE 30 Proteins,Amino Acids, SAFETY CONSIDERATIONS Be careful with the Bunsen burner flame. No mouth pipetting. The oxidase reagent is caustic. Avoid contact with eyes and skin. In case of contact,
More informationSpecies Obtained from Clinical Specimens
JOURNAL OF CINICAL MicROBiowOGy, Dec. 1976, p. 492-52 Copyright 1976 American Society for Microbiology Vol. 4, No. 6 Printed in U.S.A. Cellular Fatty Acids and Metabolic Products ofpseudomonas Species
More informationSCREENING LACTIC ACID BACTERIA FOR ANTIMICROBIAL COMPOUND PRODUCTION K. KHALISANNI, K. LEE HUNG
SCREENING LACTIC ACID BACTERIA FOR ANTIMICROBIAL COMPOUND PRODUCTION K. KHALISANNI, K. LEE HUNG Department of Microbiology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam,
More information