Immune receptors Y Y. Multiple immune responses CLR TLR NLR ITAM RLR. C-type lectin. ITAM: Immunoreceptor Tyrosine-based Activation Motif
|
|
- Adrian Norman
- 6 years ago
- Views:
Transcription
1 C
2 Immune receptors Innate immunity Acquired immunity TLR CLR FcR BCR TCR C-type lectin Y Y Ig Ig NLR ITAM ITAM ITAM RLR Multiple immune responses ITAM: Immunoreceptor Tyrosine-based Activation Motif
3 Immune receptors Innate immunity Acquired immunity TLR CLR FcR BCR TCR C-type lectin? Y Y Ig Ig NLR ITAM ITAM ITAM ITAM RLR? Multiple immune responses ITAM: Immunoreceptor Tyrosine-based Activation Motif
4 Mincle (Macrophage inducible C-type lectin) Matsumoto et al. J. Immunol M 0 LPS (ng/ml) anti-mincle Mincle C? N + R C-type lectin domain C + ITAM adaptors Mouse Rat Human LSWTIAGASILFLSGCFITRCVV LSWTMAGASILFLSVCFITRCVV FLWTVAGIPILFLSACFITRCVV + Trans-membrane region N Y Y Y Y FcR DAP10 DAP12
5 Mincle is associated with FcR by charge IP: anti-flag Mincle Mincle R42I Mincle Flag-DAP12 Flag-DAP10 Flag-FcR R D I D FcR FcR anti-mincle Mincle IP: : anti-fcr anti-fcr anti-flag WT FcR + + Mincle-Flag R42I Total lysates anti-flag mab anti-mincle anti-fcr
6 Mincle induces inflammatory cytokines Peritoneal M Mincle TLR FcR MyD88 Inflammatory cytokines
7 Mincle induces inflammatory cytokines Peritoneal M Mincle? TLR FcR MyD88 Inflammatory cytokines
8 Establishment of indicator cell lines Peritoneal M? Mincle TLR non-myeloid T cell hybridoma FcR NFAT-GFP
9 Establishment of indicator cell lines Peritoneal M Plate-coated anti-mincle Y Y Mincle TLR non-myeloid T cell hybridoma FcR NFAT-GFP anti- Mincle
10 Mincle recognizes Dead cells PI Yamasaki, et al. Nat. Immunol. 2008
11 Mincle recognizes DAMPs Damage-associated molecular patterns PI Yamasaki, et al. Nat. Immunol. 2008
12 Mincle recognizes DAMPs Damage-associated molecular patterns PAMPs Pathogen-associated molecular patterns Malassezia PI 5 μm Yamasaki, et al. Nat. Immunol Yamasaki, et al. Proc. Natl. Acad. Sci. USA. 2009
13 Mincle may be a dual sensor for the danger Danger Damaged-self Mincle Non-self Pathogens Ligand SAP130 Fungus Yamasaki, et al. Nat. Immunol Yamasaki, et al. PNAS Inflammation Physiological advantage Repair? Host Defense? Potential risk Autoimmune? Skin/Lung disease? Granulomatous disease?
14 Mincle may be a dual sensor for the danger Danger Ligand Damaged-self SAP130 Mincle Non-self Fungus Pathogens Bacteria? Yamasaki, et al. Nat. Immunol Yamasaki, et al. PNAS Inflammation Physiological advantage Repair? Host Defense? Potential risk Autoimmune? Skin/Lung disease? Granulomatous disease?
15 Mycobacterium tuberculosis Research Institute of Tuberculosis
16 Mincle recognizes mycobacteria Lab strain Mincle NFAT-GFP? Yasu Morita Taroh Kinoshita
17 Mincle recognizes mycobacteria Lab strain BCG strain Virulent strain H37Rv Yasu Morita Taroh Kinoshita Hisakata Yamada Yasunobu Yoshikai
18 Mincle recognizes mycobacteria Mycobacteria rat IgG anti-mincle Y Mincle anti-mincle NFAT-GFP Mycobacterium smegmatis
19 What is the ligand for Mincle? TDM Trehalose dimycolate GMM Glucose monomycolate LAM Lipoarabino mannan Mycolate MDP Muramyl dipeptide Arabinogalactan Peptideglycan PIM Phosphatidyl inositol mannoside Transmembrane Mycobacterial cell wall
20 What is the ligand for Mincle? TDM Trehalose dimycolate Mycolate GMM Glucose monomycolate LAM Lipoarabino mannan MDP Muramyl dipeptide Solvents - Chloroform:MeOH - Hexane - Acetone - BuOH Whole mycobacteria Arabinogalactan Peptideglycan PIM Phosphatidyl inositol mannoside Transmembrane Insoluble Soluble extract Ligand? Ligand?
21 What is the ligand for Mincle? Mycobacterium smegmatis Insoluble fractions Mincle NFAT-GFP?
22 What is the ligand for Mincle? Mycobacterium smegmatis Insoluble C:M ; Chloroform:MeOH = 9:1
23 What is the ligand for Mincle? Mycobacterium smegmatis Insoluble C:M ; Chloroform:MeOH = 9:1 Soluble fractions evaporate reporter assay coated
24 Ligand was extracted into lipophilic fraction Mycobacterium smegmatis Insoluble Soluble fractions Soluble evaporate reporter assay coated C:M ; Chloroform:MeOH = 9:1
25 Ligand was extracted into lipophilic fraction Mycobacterium smegmatis Insoluble C:M ; Chloroform:MeOH Soluble Lipid?
26 TLC (Thin Layer Chromatography) Solvent front Sample spot C:M = 9:1
27 TLC (Thin Layer Chromatography) Fraction# C:M = 9:1 Yasu Morita Tetsuaki Ishikawa
28 Active fraction contains sugar and lipid moiety Orcinol stain purple Fraction# C:M = 9:1 Yasu Morita Tetsuaki Ishikawa
29 Active fraction contains sugar and lipid moiety Orcinol stain Glycolipid Fraction# R f C:M = 9:1
30 Active fraction contains sugar and lipid moiety Orcinol stain Glycolipid TDM Trehalose dimycolate Fraction# R f C:M = 9:1
31 TDM (trehalose 6,6 dimycolate)
32 TDM (trehalose 6,6 dimycolate) - unique mycobacterial glycolipid TDM Trehalose dimycolate GMM Glucose monomycolate LAM Lipoarabino mannan Mycolate MDP Muramyl dipeptide Arabinogalactan Peptideglycan PIM Phosphatidyl inositol mannoside Transmembrane Mycobacteria
33 TDM (trehalose 6,6 dimycolate) - unique mycobacterial glycolipid - immunostimulant called cord factor
34 Kekkaku 9:521-4., 1955 Lung cavity Cord factor (= TDM)
35 TDM (trehalose 6,6 dimycolate) - unique mycobacterial glycolipid - immunostimulant called cord factor - major adjuvant component of CFA
36 TDM (trehalose 6,6 dimycolate) - unique mycobacterial glycolipid - immunostimulant called cord factor - major adjuvant component of CFA - specific receptor??
37 TDM (trehalose 6,6 dimycolate) - unique mycobacterial glycolipid - immunostimulant called cord factor - major adjuvant component of CFA - specific receptor?? Mincle FcR
38 Direct receptor?
39 Mincle is a direct receptor for TDM TDM OD450 binding nm (OD 450 nm) Y anti-higg Mincle-Ig TDM
40 Mincle is a direct receptor for TDM anti-higg Mincle-Ig TDM Y Trehalose TDM OD450 binding nm (OD 450 nm)
41 Mincle-deficient mice?
42 Mincle is essential for TDM-induced M activation BMM NO Shizuo Akira
43 In vivo response?
44 TDM-induced systemic inflammation TDM i.v. Serum (day 1)
45 TDM-induced lung inflammation TDM i.v. Lung (day 0, 1, 3, 7) Day 0 Day 1 Day 3 Day 7 H&E staining 1 mm
46 TDM-induced lung inflammation TDM i.v. Lung (day 7) Day 0 Day 7 Day 7 WT Mincle / 0.1 mm H&E staining / lung Granuloma
47 TDM-induced lung inflammation TDM i.v. Lung (day 7) Day 0 Day 7 Day 7 WT 0.1 mm Mincle /
48 Mincle mediates granuloma-forming signal in vivo TDM i.v. Lung (day 7) Mincle FcR Granuloma
49 Synthetic adjuvant, TDB TDB Trehalose dibehenate TDM Trehalose dimycolate
50 TDB acts through Mincle BMM TDB Trehalose dibehenate
51 Evolutional struggle between host and mycobacteria? Mycobacteria Host immunity
52 Evolutional struggle between host and mycobacteria? Matsunaga, et al. J. Biol. Chem Mycobacteria 3. Convert Moody, et al. Nature 2000 Host immunity
53 Evolutional struggle between host and mycobacteria? Matsunaga, et al. J. Biol. Chem Mycobacteria 3. Convert APC Host immunity Moody, et al. Nature 2000
54 Evolutional struggle between host and mycobacteria? Matsunaga, et al. J. Biol. Chem Mycobacteria 3. Escape? 3. Convert APC Host immunity Moody, et al. Nature 2000
55 In vitro mimic of bacterial conversion TDM Trehalose dimycolate Conversion GMM Glucose monomycolate Mycobacteria Mycolyl- transferease + Glucose Glucose Mycolate OH HO Glucose Glucose OH HO Glucose In vitro mimic Mycolate Trehalase Mycolate
56 GMM conversion may be an escape strategy TDM Trehalose dimycolate Conversion GMM Glucose monomycolate Glucose HO Glucose OH In vitro mimic Trehalase Mycolate Mycolate Mincle NFAT-GFP
57 Evolutional struggle between host and mycobacteria? Matsunaga, et al. J. Biol. Chem Mycobacteria 3. Escape APC Host immunity Moody, et al. Nature 2000
58 Evolutional struggle between host and mycobacteria? Matsunaga, et al. J. Biol. Chem Mycobacteria 3. Escape 3. Convert Adjuvant APC Host immunity Moody, et al. Nature 2000 Prime?
59 Acknowledgments Eri Ishikawa Tetsuaki Ishikawa Kenji Toyonaga Yasunobu Miyake Daiki Mori Masahiro Nagaat (Kyushu Univ.) Yasu Morita Taroh Kinoshita (Osaka Univ.) Machie Sakuma Takashi Saito (RCAI, RIKEN) Makoto Matsumoto Osamu Takeuchi Kiyoshi Takeda Shizuo Akira (Osaka Univ.) Hisakata Yamada Akiko Oyamada Yasunobu Yoshikai (Kyushu Univ.)
Innate immunity. Abul K. Abbas University of California San Francisco. FOCiS
1 Innate immunity Abul K. Abbas University of California San Francisco FOCiS 2 Lecture outline Components of innate immunity Recognition of microbes and dead cells Toll Like Receptors NOD Like Receptors/Inflammasome
More information2. Innate immunity 2013
1 Innate Immune Responses 3 Innate immunity Abul K. Abbas University of California San Francisco The initial responses to: 1. Microbes: essential early mechanisms to prevent, control, or eliminate infection;
More informationInnate Immunity & Inflammation
Innate Immunity & Inflammation The innate immune system is an evolutionally conserved mechanism that provides an early and effective response against invading microbial pathogens. It relies on a limited
More informationImmunology Basics Relevant to Cancer Immunotherapy: T Cell Activation, Costimulation, and Effector T Cells
Immunology Basics Relevant to Cancer Immunotherapy: T Cell Activation, Costimulation, and Effector T Cells Andrew H. Lichtman, M.D. Ph.D. Department of Pathology Brigham and Women s Hospital and Harvard
More informationRole of Innate Immunity in Control of Adaptive Immunity
Role of Innate Immunity in Control of Adaptive Immunity Innate Immunity The burden of pathogen sensing is placed on the innate immune system Danger hypothesis Missing Self Based on the detection of molecular
More informationJPEMS Nantes, Basic Immunology INNATE IMMUNITY
JPEMS Nantes, 2014- Basic Immunology INNATE IMMUNITY Teacher: Pr. Régis Josien, Laboratoire d Immunologie and INSERM U1064, CHU Nantes Regis.Josien@univ-nantes.fr 1 Contents 1. General features and specificity
More informationInnate Immunity. Hathairat Thananchai, DPhil Department of Microbiology Faculty of Medicine Chiang Mai University 2 August 2016
Innate Immunity Hathairat Thananchai, DPhil Department of Microbiology Faculty of Medicine Chiang Mai University 2 August 2016 Objectives: Explain how innate immune system recognizes foreign substances
More informationInnate Immunity: (I) Molecules & (II) Cells. Part II: Cells (aka the Sentinels)
Innate Immunity: (I) Molecules & (II) Cells Stephanie Eisenbarth, M.D., Ph.D. FOCIS Advanced Course 2/19/18 Department of Laboratory Medicine Yale School of Medicine Department of Immunobiology Yale School
More informationSponsored document from Microbes and Infection / Institut Pasteur
Sponsored document from Microbes and Infection / Institut Pasteur The Syk/CARD9-coupled receptor Dectin-1 is not required for host resistance to Mycobacterium tuberculosis in mice Mohlopheni J. Marakalala
More informationUnder the Radar Screen: How Bugs Trick Our Immune Defenses
Under the Radar Screen: How Bugs Trick Our Immune Defenses Session 3: Toll-like receptors (TLRs) Marie-Eve Paquet and Gijsbert Grotenbreg Whitehead Institute for Biomedical Research Introduction to Toll-like
More informationToll-like Receptors (TLRs): Biology, Pathology and Therapeutics
Toll-like Receptors (TLRs): Biology, Pathology and Therapeutics Dr Sarah Sasson SydPATH Registrar 23 rd June 2014 TLRs: Introduction Discovered in 1990s Recognise conserved structures in pathogens Rely
More informationInflammation: How to Cool the Fire Inside your Gut? REINVENTING DIAGNOSTICS
Inflammation: How to Cool the Fire Inside your Gut? REINVENTING DIAGNOSTICS Future of Healthcare REINVENTING DIAGNOSTICS Inflammation Gut Inflammation Basis of a Healthy
More informationStructure and Function of Antigen Recognition Molecules
MICR2209 Structure and Function of Antigen Recognition Molecules Dr Allison Imrie allison.imrie@uwa.edu.au 1 Synopsis: In this lecture we will examine the major receptors used by cells of the innate and
More informationAuthor(s) Yasunobu; Ose, Toyoyuki; Yamasaki, Sho; Maenaka, Kat. WoS_63102_Furukarwa-Mincle-MCL_KU47.pdf ( 本文 ) Instructions for use
Title Structural analysis for glycolipid recognition by th Furukawa, Atsushi; Kamishikiryo, Jun; Mori, Daiki; T Author(s) Yasunobu; Ose, Toyoyuki; Yamasaki, Sho; Maenaka, Kat CitationProceedings of the
More informationSelf and nonself recognition through C-type lectin receptor, Mincle
九州大学学術情報リポジトリ Kyushu University Institutional Repository Self and nonself recognition through C-type lectin receptor, Mincle Miyake, Yasunobu Division of Molecular Immunology, Medical Institute of Bioregulation,
More informationGout and Nucleic Acid Metabolism Vol.33 No
Gout and Nucleic Acid Metabolism Vol.33 No.1 2009 1 1 2 3 in vitro 14 IgM 1 IgM IgM 1 PAMPs Pattern recognition receptors PRRs PRRs PRRs PAMPs Toll Toll-like receptor TLR PAMPs Nod Nod-like receptor NLR
More informationImmunology Lecture 4. Clinical Relevance of the Immune System
Immunology Lecture 4 The Well Patient: How innate and adaptive immune responses maintain health - 13, pg 169-181, 191-195. Immune Deficiency - 15 Autoimmunity - 16 Transplantation - 17, pg 260-270 Tumor
More informationEffect of Mold Constituents on
Effect of Mold Constituents on Innate Immune Responses (Alphabet Soup for Fungal Exposure) Charles S. Barnes, Ph.D. Children s Mercy Hospitals and Clinics The Fungal (fifth) Kingdom Fungi include mold,
More informationInnate immune regulation of T-helper (Th) cell homeostasis in the intestine
Innate immune regulation of T-helper (Th) cell homeostasis in the intestine Masayuki Fukata, MD, Ph.D. Research Scientist II Division of Gastroenterology, Department of Medicine, F. Widjaja Foundation,
More informationMechanism of the adjuvant activity of the synthetic mycobacterial cord factor analog Trehalose-6,6-dibehenate (TDB)
TECHNISCHE UNIVERSITÄT MÜNCHEN Lehrstuhl für Biofunktionalität der Lebensmittel Mechanism of the adjuvant activity of the synthetic mycobacterial cord factor analog Trehalose-6,6-dibehenate (TDB) Kerstin
More informationAdaptive Immunity to Bacteria. T cell subsets
Adaptive Immunity to Bacteria Role of T cells in anti-bacterial host responses. Dr. C. Piccirillo Department of Microbiology & Immunology McGill University T cell subsets MHC I and II -restricted cells
More informationLecture on Innate Immunity and Inflammation. Innate Immunity: An Evolutionary View
Lecture on Innate Immunity and Inflammation Evolutionary View Epithelial barriers to infection Four main types of innate recognition molecules:tlrs, CLRs, NLRs, RLRs NF-κB, the master transcriptional regulator
More informationSUPPLEMENTARY INFORMATION
In the format provided by the authors and unedited. SUPPLEMENTARY INFORMATION VOLUME: 2 ARTICLE NUMBER: 17084 Metabolic anticipation in Mycobacterium tuberculosis Hyungjin Eoh, Zhe Wang, Emilie Layre,
More informationToll-like Receptor Signaling
Toll-like Receptor Signaling 1 Professor of Medicine University of Massachusetts Medical School, Worcester, MA, USA Why do we need innate immunity? Pathogens multiply very fast We literally swim in viruses
More informationPutting it Together. Stephen Canfield Secondary Lymphoid System. Tonsil Anterior Cervical LN s
Putting it Together Stephen Canfield smc12@columbia.edu Secondary Lymphoid System Tonsil Anterior Cervical LN s Axillary LN s Mediastinal/Retroperitoneal LN s Thoracic Duct Appendix Spleen Inguinal LN
More informationChapter 3 The Induced Responses of Innate Immunity
Chapter 3 The Induced Responses of Innate Immunity Pattern recognition by cells of the innate immune system Pattern recognition by cells of the innate immune system 4 main pattern recognition receptors
More informationInnate Immunity. Hathairat Thananchai, DPhil Department of Microbiology Faculty of Medicine Chiang Mai University 25 July 2017
Innate Immunity Hathairat Thananchai, DPhil Department of Microbiology Faculty of Medicine Chiang Mai University 25 July 2017 Objectives: Explain how innate immune system recognizes foreign substances
More informationToll-Like Receptor 9 Is Required for Full Host Resistance to Mycobacterium avium Infection but Plays No Role in Induction of Th1 Responses
INFECTION AND IMMUNITY, Apr. 2011, p. 1638 1646 Vol. 79, No. 4 0019-9567/11/$12.00 doi:10.1128/iai.01030-10 Copyright 2011, American Society for Microbiology. All Rights Reserved. Toll-Like Receptor 9
More informationInhibition of Mycolic acid transport across the Mycobacterium tuberculosis plasma membrane
Inhibition of Mycolic acid transport across the Mycobacterium tuberculosis plasma membrane Grzegorzewicz, A. E., H. Pham, V. A. K. B. Gundi, M. S. Scherman, E. J. North, T. Hess, V. Jones, V. Gruppo, S.
More informationLecture on Innate Immunity and Inflammation
Lecture on Innate Immunity and Inflammation Evolutionary View Epithelial barriers to infection Four main types of innate recognition molecules:tlrs, CLRs, NLRs, RLRs NF-κB, the master transcriptional regulator
More informationHost recognition of fungal pathogens
Drug Discovery Today: Disease Mechanisms Vol. 4, No. 4 2007 DRUG DISCOVERY TODAY DISEASE MECHANISMS Editors-in-Chief Toren Finkel National Heart, Lung and Blood Institute, National Institutes of Health,
More informationC-Type Lectin Receptor MCL Facilitates Mincle Expression and Signaling through Complex Formation
This information is current as of December 26, 2018. C-Type Lectin Receptor MCL Facilitates Mincle Expression and Signaling through Complex Formation Yasunobu Miyake, Masatsugu Oh-hora and Sho Yamasaki
More informationImmunology Part II. Innate Immunity. 18. April 2018, Ruhr-Universität Bochum Marcus Peters,
Immunology Part II Innate Immunity 18. April 2018, Ruhr-Universität Bochum Marcus Peters, marcus.peters@rub.de Conserved structures of pathogens PAMPs are detected by Pattern Recognition Receptors PRRs
More informationExosomes function in antigen presentation during an in vivo Mycobacterium tuberculosis infection
Exosomes function in antigen presentation during an in vivo Mycobacterium tuberculosis infection Victoria L. Smith, Yong Cheng, Barry R. Bryant and Jeffrey S. Schorey Supplementary Figure 1: Unprocessed
More informationPhagocytosis: An Evolutionarily Conserved Mechanism to Remove Apoptotic Bodies and Microbial Pathogens
Phagocytosis of IgG-coated Targets by s Phagocytosis: An Evolutionarily Conserved Mechanism to Remove Apoptotic Bodies and Microbial s 3 min 10 min Mast Cells Can Phagocytose Too! Extension of an F-actin-rich
More informationInnate Immunity: (I) Molecules & (II) Cells
Innate Immunity: (I) Molecules & (II) Cells Stephanie Eisenbarth, M.D., Ph.D. FOCIS Advanced Course 2/19/18 Department of Laboratory Medicine Yale School of Medicine Department of Immunobiology Yale School
More informationTD-BF01: Innate immunity to microorganisms
TD-BF01: Innate immunity to microorganisms I. Toll receptors (adapted from Takeuchi, O. et al. (1999) Immunity 11:443; Kawai, T. et al. (1999) Immunity 11:115; Hemmi, H. et al. (2000) Nature 408:740; Muzio,
More informationNewly Recognized Components of the Innate Immune System
Newly Recognized Components of the Innate Immune System NOD Proteins: Intracellular Peptidoglycan Sensors NOD-1 NOD-2 Nod Protein LRR; Ligand Recognition CARD RICK I-κB p50 p65 NF-κB Polymorphisms in Nod-2
More informationDOWNLOAD OR READ : LIPOPOLYSACCHARIDE ENDOTOXIN INDUCES IL 18 VIA CD14 IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS IN VITRO PDF EBOOK EPUB MOBI
DOWNLOAD OR READ : LIPOPOLYSACCHARIDE ENDOTOXIN INDUCES IL 18 VIA CD14 IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS IN VITRO PDF EBOOK EPUB MOBI Page 1 Page 2 lipopolysaccharide endotoxin induces il 18
More informationReview Article Nonprotein Structures from Mycobacteria: Emerging Actors for Tuberculosis Control
Clinical and Developmental Immunology Volume 2012, Article ID 917860, 9 pages doi:10.1155/2012/917860 Review Article Nonprotein Structures from Mycobacteria: Emerging Actors for Tuberculosis Control Luz
More informationBasic immunology. Lecture 7. Innate immunity, pattern recognition. Péter Engelmann
Basic immunology Lecture 7. Innate immunity, pattern recognition Péter Engelmann Different levels of the immune response Recognition molecules of the innate immunity The levels of host defense Anatomical
More informationIntracellular MHC class II molecules promote TLR-triggered innate. immune responses by maintaining Btk activation
Intracellular MHC class II molecules promote TLR-triggered innate immune responses by maintaining Btk activation Xingguang Liu, Zhenzhen Zhan, Dong Li, Li Xu, Feng Ma, Peng Zhang, Hangping Yao and Xuetao
More informationSiglec-15 Is A Potential Therapeutic Target For Postmenopausal Osteoporosis
Siglec-15 Is A Potential Therapeutic Target For Postmenopausal Osteoporosis Yusuke Kameda, Masahiko Takahata, Tomohiro Shimizu, Hiroki Hamano, Norimasa Iwasaki. Department of Orthopedic Surgery, Hokkaido
More informationFelix Yarovinsky. Department of Immunology, UT Southwestern Medical Center. Innate immune defense to Toxoplasma gondii
Felix Yarovinsky Department of Immunology, UT Southwestern Medical Center Innate immune defense to Toxoplasma gondii Pathogen recognition by innate immune cells Pathogen Parasites Viruses Bacteria Initiator
More informationActivation of inflammatory response by fungal cell wall components and toxins
Finnish Institute of Occupational Health Activation of inflammatory response by fungal cell wall components and toxins Sampsa Matikainen Innate Immunity Research Group Unit of Immunotoxicology Finnish
More informationInnate Immunity II. Integration. Lindsay Nicholson Advanced Immunology L2
Innate Immunity II Integration Lindsay Nicholson Advanced Immunology L2 l.nicholson@bristol.ac.uk Lecture 1 Defining Innate Immunity Recognition and effector mechanisms (I) Lecture 2 Recognition and effector
More informationIn Vivo Activity of Released Cell Wall Lipids. Calmette-Guérin Is Due Principally to Trehalose Mycolates
This information is current as of November 11, 2018. References Subscription Permissions Email Alerts In Vivo Activity of Released Cell Wall Lipids of Mycobacterium bovis Bacillus Calmette-Guérin Is Due
More informationReview Article Pattern Recognition Receptors and Cytokines in Mycobacterium tuberculosis Infection The Double-Edged Sword?
BioMed Research International Volume 2013, Article ID 179174, 18 pages http://dx.doi.org/10.1155/2013/179174 Review Article Pattern Recognition Receptors and Cytokines in Mycobacterium tuberculosis Infection
More informationIdentification of distinct ligands for the C-type lectin receptors Mincle and Dectin-2 in the pathogenic fungus Malassezia
Identification of distinct ligands for the C-type lectin receptors Mincle and Dectin-2 in the pathogenic fungus Malassezia Running title: Fungal ligands for Mincle and Dectin-2 Tetsuaki Ishikawa 1, Fumie
More informationIndex. Note: Page numbers of article titles are in boldface type.
Note: Page numbers of article titles are in boldface type. A Adaptive immune response biologic response modifiers and, 735 737 S-Adenosylmethionine (SAMe) for hepatitis, 825 826 Albinterferon for hepatitis,
More information1. TLR. TLR Toll-like receptors. Toll Toll-like receptor, TLR TLR TLR TLR. type I TLR TLR. Toll
54pp.145 152 2004 1. TLR T B TLR Toll-like receptors TLR TLR I IFN TLR T B B T Toll NF- B 1996 565-0871 3-1 TEL 06-6879-8303 FAX 06-6879-8305 E-mail uemattsu@biken.osaka-u.ac.jp Toll Toll-like receptor,
More informationTOLL-LIKE RECEPTORS AND CYTOKINES IN SEPSIS
TOLL-LIKE RECEPTORS AND CYTOKINES IN SEPSIS A/PROF WILLIAM SEWELL ST VINCENT S CLINICAL SCHOOL, UNSW SYDPATH, ST VINCENT S HOSPITAL SYDNEY GARVAN INSTITUTE INNATE VERSUS ADAPTIVE IMMUNE RESPONSES INNATE
More informationTime course of immune response
Time course of immune response Route of entry Route of entry (cont.) Steps in infection Barriers to infection Mf receptors Facilitate engulfment Glucan, mannose Scavenger CD11b/CD18 Allows immediate response
More informationD2 inhibits TLR2- initiated 12p40 transcription (-) TLR2 PGN MDP. MyD88 IRAK ECSIT TRAF6 NIK. Smallest unit of PGN muramyl dipeptide IKK.
D2 inhibits TLR2- initiated 12p40 transcription CARD CARD NOD2 LRR RICK/Rip2 NIK MDP TRAF6 PGN TLR2 MyD88 IRAK ECSIT (-) IKK Smallest unit of PGN muramyl dipeptide IκB NF-κB atanabe et al, 2004 NF-κB IL-12p40
More informationCD4+ T Helper T Cells, and their Cytokines in Immune Defense and Disease
CD4+ T Helper T Cells, and their Cytokines in Immune Defense and Disease Andrew Lichtman M.D., Ph.D. Brigham and Women s Hospital Harvard Medical School Lecture outline Intro to T cell mediated immunity
More informationBacterial immunostat: Mycobacterium tuberculosis lipids and their role in the host immune response
Rev Soc Bras Med Trop 50(1):9-18, January-February, 2017 doi: 10.1590/0037-8682-0230-2016 Review Article Bacterial immunostat: Mycobacterium tuberculosis lipids and their role in the host immune response
More informationThe Role of Rv2623 in Deciphering the Mechanisms of Tuberculosis Latency. By: Amulya Marellapudi Nyack High School
The Role of Rv2623 in Deciphering the Mechanisms of Tuberculosis Latency By: Amulya Marellapudi Nyack High School Abstract: Mycobacterium tuberculosis (Mtb), the bacterium that causes tuberculosis (TB),
More informationchapter 17: specific/adaptable defenses of the host: the immune response
chapter 17: specific/adaptable defenses of the host: the immune response defense against infection & illness body defenses innate/ non-specific adaptable/ specific epithelium, fever, inflammation, complement,
More informationComparison of Two Mice Strains, A/J and C57BL/6, in Caspase-1 Activity and IL-1
Mediators of Inflammation Volume, Article ID 7873, 5 pages doi:.55//7873 Research Article Comparison of Two Mice Strains, A/J and C57BL/6, in Caspase- Activity and IL-β SecretionofMacrophage to Mycobacterium
More informationAnti-Ly9 (CD229) antibody treatment reduces marginal zone B cell numbers and salivary gland inflammation in a mouse model of Sjögren s Syndrome
Anti-Ly9 (CD229) antibody treatment reduces marginal zone B cell numbers and salivary gland inflammation in a mouse model of Sjögren s Syndrome Joan Puñet-Ortiz, Manuel Sáez Moya, Marta Cuenca, Adriana
More informationThe Role of Syk/CARD9 Coupled C-type Lectins in Antifungal Immunity
The Role of Syk/CARD9 Coupled C-type Lectins in Antifungal Immunity Rebecca A. Drummond * 1, Shinobu Saijo* 2, Yoichiro Iwakura 2, and Gordon D. Brown 1 *These authors contributed equally to the writing
More informationT cell and Cell-mediated immunity
T cell and Cell-mediated immunity ( 第十章 第十二章第十二章 ) Lu Linrong ( 鲁林荣 ) PhD Laboratory of Immune Regulation Institute of Immunology Zhejiang University, School of Medicine Medical Research Building B815-819
More informationInnate Immunity. Jan 8 th Prof. dr. sc. Ivana Novak Nakir 1
Innate Immunity Jan 8 th 2018. Prof. dr. sc. Ivana Novak Nakir 1 Adaptive Innate 2 Immune system overview 1 st line of defense skin (2m 2 ) and mucosal membranes (~400m 2 ): physical barrier, lymphoid
More informationIdentification of Microbes
Identification of Microbes Recognition by PRR (pattern recognition receptors) Recognize conserved molecular patterns on microbes called pathogen associated molecular patterns (PAMPs) which are not present
More informationNovel function of NADPH oxidase in atherosclerosis. Yun Soo Bae Department of Life Science Ewha Womans University
Novel function of NADPH oxidase in atherosclerosis Yun Soo Bae Department of Life Science Ewha Womans University Recent understanding of ROS: act as second messengers e e Catalase/peroxidase O 2 H 2 O
More informationIntegrin CD11b negatively regulates TLR-triggered inflammatory responses by. activating Syk and promoting MyD88 and TRIF degradation via cbl-b
Integrin CD11b negatively regulates TLR-triggered inflammatory responses by activating Syk and promoting MyD88 and TRIF degradation via cbl-b Chaofeng Han, Jing Jin, Sheng Xu, Haibo Liu, Nan Li, and Xuetao
More informationHost-Pathogen Interactions in Tuberculosis
Host-Pathogen Interactions in Tuberculosis CNRS - Toulouse, France My presentation will focus on host-cell pathogen interactions in tuberculosis. However, I would first like offer a brief introduction
More informationMedical Bacteriology- Lecture 10. Mycobacterium. Actinomycetes. Nocardia
Medical Bacteriology- Lecture 10 Mycobacterium Actinomycetes Nocardia 1 Mycobacterium Characteristics - Large, very weakly gram positive rods - Obligate aerobes, related to Actinomycetes - Catalase positive
More informationVMC-221: Veterinary Immunology and Serology (1+1) Question Bank
VMC-221: Veterinary Immunology and Serology (1+1) Objective type Questions Question Bank Q. No. 1 - Fill up the blanks with correct words 1. The British physician, who developed the first vaccine against
More informationINFLAMMASOME IN INTESTINAL INFLAMMATION AND CANCER. Laura Stronati ENEA - Roma
INFLAMMASOME IN INTESTINAL INFLAMMATION AND CANCER Laura Stronati ENEA - Roma Inflammasome: definition, components and activation TLRs NODs RLRs CLRs PRRs LPS, Flagellin, DNA, RNA, etc) PAMPs DAMPs ATP,
More informationA Slfn2 mutation causes lymphoid and myeloid immunodeficiency due to loss of immune cell quiescence
Supplementary Information A Slfn mutation causes lymphoid and myeloid immunodeficiency due to loss of immune cell quiescence Michael Berger, Philippe Kres, Karine Crozat, Xiaohong Li, Ben A. Croker, Owen
More informationHelminth worm, Schistosomiasis Trypanosomes, sleeping sickness Pneumocystis carinii. Ringworm fungus HIV Influenza
Helminth worm, Schistosomiasis Trypanosomes, sleeping sickness Pneumocystis carinii Ringworm fungus HIV Influenza Candida Staph aureus Mycobacterium tuberculosis Listeria Salmonella Streptococcus Levels
More informationSupporting Information
Supporting Information Bansal-Mutalik and Nikaido 10.1073/pnas.1112572108 Relative lipid release (% ) Relative lipid release (%) 15 30 60 120 240 1440 Extraction time (minutes) Step 1 Step 2 Step 3 Number
More informationA Yersinia-Secreted Effector Protein Promotes Virulence by Preventing Inflammasome Recognition of the Type III Secretion System
Cell Host & Microbe, Volume 7 Supplemental Information A Yersinia-Secreted Effector Protein Promotes Virulence by Preventing Inflammasome Recognition of the Type III Secretion System Igor E. Brodsky, Noah
More informationMincle suppresses Toll-like receptor 4 activation
Article Mincle suppresses Toll-like receptor 4 activation Stephanie H. Greco,* Syed Kashif Mahmood,* Anne-Kristin Vahle,* Atsuo Ochi,* Jennifer Batel, Michael Deutsch,* Rocky Barilla,* Lena Seifert,* H.
More informationChapter 11. B cell generation, Activation, and Differentiation. Pro-B cells. - B cells mature in the bone marrow.
Chapter B cell generation, Activation, and Differentiation - B cells mature in the bone marrow. - B cells proceed through a number of distinct maturational stages: ) Pro-B cell ) Pre-B cell ) Immature
More informationThe Innate Immune Response is Conserved Throughout Evolution and is Triggered by Pattern Recognition. Lipopolysaccharide = Lipid + Polysaccharide
The Innate Immune Response is Conserved Throughout Evolution and is Triggered by Pattern Recognition Lipopolysaccharide = Lipid + Polysaccharide E.coli Cell wall organization Lipopolysaccharide Outer membrane
More informationChapter 1. Chapter 1 Concepts. MCMP422 Immunology and Biologics Immunology is important personally and professionally!
MCMP422 Immunology and Biologics Immunology is important personally and professionally! Learn the language - use the glossary and index RNR - Reading, Note taking, Reviewing All materials in Chapters 1-3
More informationSUPPLEMENTARY MATERIAL
SUPPLEMENTARY MATERIAL IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the T H 17 cell it reg cell balance Rajatava Basu 1,5, Sarah K.
More informationACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY
ACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY The recognition of specific antigen by naïve T cell induces its own activation and effector phases. T helper cells recognize peptide antigens through
More informationOverview of the Immune System
Overview of the Immune System Immune System Innate (Nonspecific) Adaptive (Specific) Cellular Components Humoral Components Cell-Mediated Humoral (Ab) Antigens Definitions Immunogen Antigen (Ag) Hapten
More informationMedical Bacteriology- lecture 13. Mycobacterium Actinomycetes
Medical Bacteriology- lecture 13 Mycobacterium Actinomycetes Mycobacterium tuberculosis Large, very weakly gram positive rods, Obligate aerobes, related to Actinomycetes, non spore forming, non motile
More informationMincle and human B cell function
Mincle and human B cell function Kazuhito Kawata, University of California Davis Petr Illarionov, University of Birmingham Thomas Kenny, University of California Davis Weici Zhang, University of California
More informationJournal club. Lama Nazzal
Journal club Lama Nazzal Background Kidney stone disease affects about 12% of men and 5% of women during their lifetimes in the United States Intrarenal nephrocalcinosis is often asymptomatic, but can
More informationMycobacterial infection: Immune evasion, host susceptibility and immunological markers of diagnostic importance
Doctoral thesis from the Department of Immunology, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden Mycobacterial infection: Immune evasion, host susceptibility and immunological markers
More informationThe Role of KEAP1 in Regulating Inflammation and Antigen-Presentation in Response to Mycobacterium avium Infection in Macrophages
Christina Dybdrodt Bjørnvall The Role of KEAP1 in Regulating Inflammation and Antigen-Presentation in Response to Mycobacterium avium Infection in Macrophages Master s Thesis Trondheim, June 2014 Principle
More informationCell Mediated Immunity CELL MEDIATED IMMUNITY. Basic Elements of Cell Mediated Immunity (CMI) Antibody-dependent cell-mediated cytotoxicity (ADCC)
Chapter 16 CELL MEDIATED IMMUNITY Cell Mediated Immunity Also known as Cellular Immunity or CMI The effector phase T cells Specificity for immune recognition reactions TH provide cytokines CTLs do the
More informationAllergy and Immunology Review Corner: Chapter 11 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti, MD.
Allergy and Immunology Review Corner: Chapter 11 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti, MD. Chapter 11: Advanced in Clinical Immunomodulation Prepared by
More informationChapter 11. B cell generation, Activation, and Differentiation. Pro-B cells. - B cells mature in the bone marrow.
Chapter B cell generation, Activation, and Differentiation - B cells mature in the bone marrow. - B cells proceed through a number of distinct maturational stages: ) Pro-B cell ) Pre-B cell ) Immature
More informationFc receptors, phagocytosis role 128
Subject Index Adaptive immunity dependence on innate immunity 9, 10 evolution 10 Aging anti-inflammatory agents in counteraction 202 beneficial polymorphisms 199 201 definition 18, 189 innate immunity
More informationReview Article Biosynthesis and Virulent Behavior of Lipids Produced by Mycobacterium tuberculosis: LAM and Cord Factor: An Overview
SAGE-Hindawi Access to Research Biotechnology Research International Volume 2011, Article ID 274693, 7 pages doi:10.4061/2011/274693 Review Article Biosynthesis and Virulent Behavior of Lipids Produced
More informationMouse Total IgA Antibody Detection Kit
Mouse Total IgA Antibody Detection Kit Catalog # 3019 For Research Use Only - Not Human or Therapeutic Use INTRODUCTION The total IgA levels in specimens are often determined in mouse disease models involving
More informationOverview of the immune system
Overview of the immune system Immune system Innate (nonspecific) 1 st line of defense Adaptive (specific) 2 nd line of defense Cellular components Humoral components Cellular components Humoral components
More informationInflammation - Molecular events
Academic lectures for students of medical schools 3rd Year updated 2004-2015 GENERAL PATHOPHYSIOLOGY Inflammation - Molecular events R. A. Benacka, MD, PhD Department of Pathophysiology Medical faculty
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationPathogen Strategies to Evade Innate Immune Response: A Signaling Point of View
6 Pathogen Strategies to Evade Innate Immune Response: A Signaling Point of View Bruno Miguel Neves 1,2, Maria Celeste Lopes 1 and Maria Teresa Cruz 1 1 Faculty of Pharmacy and Centre for Neuroscience
More informationstat6-dependent inhibition of Mincle expression in Mouse and human antigen-presenting cells by the Th2 cytokine il-4
Original Research published: 14 October 2016 doi: 10.3389/fimmu.2016.00423 stat6-dependent inhibition of Mincle expression in Mouse and human antigen-presenting cells by the Th2 cytokine il-4 Thomas Hupfer
More informationCharacteristics of Mycobacterium
Mycobacterium Characteristics of Mycobacterium Very thin, rod shape. Culture: Aerobic, need high levels of oxygen to grow. Very slow in grow compared to other bacteria (colonies may be visible in up to
More information