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Transcription

1 6,,6 6,6 (p<.5).() fight or flight 9 (6 5 Corticosteroid.(7 Adaptaion reaction) 8

2 Pot.EDTA Battery brooder (Schalm et al. 975) Burton and Guion 8 8 Electrophoresis (CT) 6 CCID 5 9 ) Merial

3 P<.5 9. c b c b c b b.8. bc b. 9. a a. 6.5 a.7. a..7 b..8 b..6 b a a.9. a.7.7 c.. a. 8. a a bc.. a. 9. a a a.. a..8 a a. 6. a a.9.5 ab..5 a. ()

4 (P<.5 P<.5 P<.5 P<.5 () (, () () (7,6) (8) (9). (p<.5) ( gluconeogenesis ) (,9 )

5 - Cosgrove, A.S., (96). An apparently new disease of chickens avian nephrosis. Avian Dis., 6: Faragher, J.T. (97). Infectious bursal disease of chickens. Vet. Bull., : Williams, N.S., (98). Stress and behavior of domestic fowl. Wld`s Poultry Sci. J., : Ballanti, J.A. (97). Immunology. W.B. Saunders company.philadelphia London. Toronto Freeman, B. M., (976). Stress and the domestic fowl: A physiological reappraisal. Wld`s poultry Sci. J., : Siegel, H.S., (98). Physiological stress in birds. Bioscience. : Schalm, O.N. Jain, and E.Carrol (975): Veterinary Hematology, rd. ed. Lea and Febiger, Philadelphia. - Coles, E.H., (97). Veterinary Clinical Pathology. th. Ed. W.B. Saunders company. Philadelphia. London Hong Kong. - Archer, R.K. (965). Hematological Techniques for use on animals Oxford Blackwell Scientific Publications. - Natt, M.P. and C. A. Herrick (95). Anew blood diluent for counting the erythrocytes and leukocytes of the chicken. Poultry Sci. : Shen, P. F. and L. T. Patterson, (98). A simplified Wright`s stain technique for routine avian blood smear staining. Poultry Sci. 6 : Burton, R.R. and C. W. Guion, (968). The differential leukocyte blood count. It`s precision and individuality in the chicken. Poultry Sci., 7 : Asatoor, A. M. and E. J. King, (95). Simplified colorimetric blood sugar method. Biochem., J. 56 : XLIV. 8- Kachmar, J. F., (97). Protein and amino acids. In : Fundamentals of clinical chemistry. N. W. Tietz (ed). W. B. Saunders Company, Philadelphia. 9- Steel, R. G. D. and J. H. Torrie, (98). Principles and procedures of statistics A Biometrical Approach nd. ed. : Mc Graw Hill book company, New York. - Styrkie, P. D. (). Avian Physiology. 5th. ed. New York. ACADMIC press. - Cochrane, C. G., (977). Role of granulocytes in immune complex

6 induced tissue injuries. Inflammation, : Gross, W. B. and H. S. Siegel, (98). Evaluation of initial and secondary period fasting rations and body weight. Avian Dis., : Mcfarlane, J. M. and S. E. Curtis. (989). Multiple concurrent stressors in chicks. Effects of plasing corticosterone on the heterophil / lymphocyte ratio poultry Sci. 68 : Siegel, H. S. and P. B. Siegel, (966). Genetic variation in responses to repeated administrations of ACTH and hydrocortisone in immature chickens. Poultry Sci. 5 : Cahaner, A. and F. Leenstra, (99). Effects of high temperature on growth and feed efficiency of male and female broilers from lines selected for high weight gain. Favorable feed conversion and high or low fat content. Poultry Sci., 7 : Trout, J. M., M. M. Mashaly and H. S. Siegel, (988). Changes in the profile of circulating white blood cells corticosterone, T and T during the initiation of humoral immunity in immature male chickens. Development and comparative immunology. : Edens, F. W. and H. S. Siegel, (976). Modification of corticosterone and glucose response by sympatholytic agents in young chickens during acute heat exposure. Poultry Sci. 55 : Kandutsch, A. H. Chen and J. Heiniger, (978). Biological activity of same oxygenated steroids science, : Kaplan, L. A. and A. J. Pesce, (989). Clinical chemistry theory analysis and correlation. nd ed. Mosby company, Toronto. - O`Riordan, J. L. H., P. G. Malan and R. P. Gould, (98). Essentials of endocrinology, Blackwell Scientific publications, London. The effect of repeated vaccination against Infectious Bursal Disease on some physiological and hematological parameters in chicks A.A.H.Shalash* F.F.Ahmed A.M.Hussain *Department of pathology and poultry College of Veterinary Medicine. University of Baghdad. Abstract The present investigation aimed at study effect of repeated vaccination against Infectious Bursal Disease (I.B.D.) on some physiological, metabolic and hematological parametrs. Sixty chicks, one day old were equally divided into four groups (G, G, G, G) and treated as follows: G: was left unvaccinated against (I.B.D.)as a control group. G: was vaccinated once against (I.B.D.) on 6 day of age. G: was vaccinated twice against (I.B.D.)on 6 and day of age. G: was vaccinated against (I.B.D.)on 6,, day of age.

7 Two weeks after the third vaccination blood samples were collected and used for estimation of total leukocyte count, heterophil and lymphocytes numbers and heterophil to lymphocyte ratio. Also estimated hemoglobin concentration packed cells volume and estimation of glucose, cholesterol, total protein, albumin and globulin concentration. Total leukocyte counts significantly (p<.5) increased in vaccinated groups compared to the control. The heterophil to lymphocyte ration continued to decrease significantly (p<.5) with increasing number of vaccination. Glucose concentrations, cholesterol levels, and total protein values showed a tendency toward reduction with increasing number of vaccinations in vaccinated groups compared with control. We conclude that repeated vaccination against (I.B.D.) induce increase in leukocytes and lymphocytes. The decreasing heterophil to lymphocyte (stress factor) in vaccinated groups might be used to explain that increasing number of vaccinations against (I.B.D.) did not expose bird to any stress or noticeable changes in physiological and Metabolic parameters.