Virus Diseases Sub-Group Report. CORESTA Meeting, Izmir
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1 Virus Diseases Sub-Group Report CORESTA Meeting, Izmir
2 SG History SG Collaborative study on PVY ( ) Originally set up in 1995 Assessing in the field a set of inbred lines for PVY virus symptoms Interim report presented at Bucharest, 2003 Collaborative experiment pursued SG Collaborative study on virus diseases ( ) 2011: last year of the collaborative study in the field SG virus diseases (VIR) since 2013
3 Objectives Objectives: 1. To compare the viral data (1996 to 2011) with climatic / aphid data. To synthetize the viral data obtained and present results in a final report 2. To promote sharing of experience in coping with tobacco virus diseases. Coordinator & Liaison: Jean-Louis VERRIER, Bergerac Seed & Breeding, France
4 Collaborative Experiment Important & complex data set Field trials with data: 292 Countries: 29 o Plus description data: Location, Date of transplantation, Date of reading, etc. Interesting due to The differential host series 7 lines constantly studied 15 years The geographic extension and duration Studies concerning the duration of resistances
5 7 lines tested 15 years vava VaVa Balanced by tobacco type and presence-absence of va
6 Initial steps to complete objective 1
7 Steps to complete objective 1
8 Importance of data «cleaning» and selecting Discarded due to inconsistent results: 9 tests PVY study (resistance breaking) 39 tests: no to low PVY symptoms Discarded for this purpose Reaction of lines to CMV All tests with no CMV symptoms discarded Etc
9 Purpose of report and study Presentation of the experiment 1. Virus occurrence and distribution 2. Reaction of the tested lines to viruses 3. Evolution of viruses Annexes Collaborative virus study map of the synthetic report List of participant organizations Information regarding the differential host series Detailed results by geographic area
10 Collaborative study synthetic report map Virus occurrence and distribution PVY, CMV, TMV, TSWV, other PVY: variations of occurrence o Low and high years in Europe Synthesis by geographic area
11 Collaborative study synthetic report map Reaction of the tested lines to viruses PVY (VaVa and vava lines) CMV Other Comment for each line
12 Collaborative study synthetic report map Evolution of viruses PVY Evolution of «va» breaking strains, o o Europe Africa and Asia
13 PVY necrotic symptoms / VaVa line L2PVYne = Log 2 (frequency of PVY necrotic symptoms) L2PVYne 4,2 4 3,8 3,6 3,4 3,2 resistant BEY21 HAB92 VaVa lines Ky17 K326 MN944 NC95 NCTG52 Selected tests VaVa lines not all equivalent While all susceptible
14 PVY necrotic symptoms / vava line L2PVYne = Log 2 (frequency of PVY necrotic symptoms) susceptible L2PVYne 1,48 1,28 1,08 0,88 0,68 resistant PBD6 TN86 vava lines VAM VD WIS Selected tests vava lines not all equivalent In line with other findings
15 CMV susceptible % CMV symptoms resistant Over all tests starting in 2003 BEY21 HAB92 K17 K326 NC95 PBD6 TN86 VAM VD VRG2 VTA WIS VaVa vava PVY: S PVY: res Slight differences among lines Not less symptoms on vava To be considered by tobacco type
16 Variations of PVY incidence by country, Europe No test in 2008 Increase of % N on vava?
17 Acknowledgements Thanks to all participants in the Collaborative Experiment Thanks to the former Coordinator Teresa Doroszewska
Technical Report. Tobacco Virus Collaborative Study ( )
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