Request for Diagnostic Testing Pig / Swine

Transcription

1 Request for Diagnostic Testing Pig / Swine Testing laboratory accredited by the DAkkS according to DIN EN ISO/IEC To: IVD GmbH Innovative Veterinary Diagnostic Laboratory Albert-Einstein-Str Seelze-Letter GERMANY : + 49 (0) : -99 E Mail: service@ivd-gmbh.de Veterinarian / Sender: Address of animal owner: Other: Vet-ID-Nr.: (for Salmonella monitoring Qualiproof by QS) VVVO-Nr.: (for Salmonella monitoring Qualiproof by QS) Results to: Veterinarian by by fax Animal owner by by fax Others by by fax Invoice only to: Veterinarian / Sender by by fax Sampling date: Type of operation: breeding: sows boars multiplier production of gilts production of boars mast: farrowing operation nursery finishing operation closed system Herd size: number of sows / boars: number of piglets: number of fatteners: Symptoms: no symptoms (health check) respiratory enteric reproductive neurological other: Clinical history: Animals treated: no yes For how long / last on: with: Animals vaccinated: PRRSV no yes last vaccination on: vaccine: PCV2 no yes last vaccination on: vaccine: M. hyopneumoniae no yes last vaccination on: vaccine: Other: no yes last vaccination on: vaccine: Location and date Signature of veterinarian / sender The current price list is available on request. All services are subject to our General Terms and Conditions of Sale, which can be reviewed at Our privacy policy can be found at Diagnostic request form_swine50_eng_ page 1 of 5

2 Sample animal ID / bar code material * sex / age ** Sample animal ID / bar code material * sex / age ** * blood (serum, plasma), BALF, organs, urine, feces, etc. ** sow (s, gilt); boar (b); piglet (p): suckling piglet (sp), weaning piglet (wp), growing pig (gp); fattening pig (f) Diagnostic request form_swine50_eng_ page 2 of 5

3 Serological tests for specific agents (Detection of antibodies) AKV (Virus of Aujeszky s Disease or Pseudorabies also PRV) ELISA App (Actinobacillus pleuropneumoniae) ApxIV ELISA App LPS Mix ELISA App ELISA virulent plus screening: highly virulent serotypes 1/9/11, 5a/b & 2 (dominant in EU) App ELISA full screening: all 12 serotypes App ELISA serotypes 1/9/11 App ELISA serotype 2 App ELISA serotypes 3/6/8 App ELISA serotypes 4/7 PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) ELISA PRRSV ELISA (alternative) PRRSV OF ELISA for oral fluids only Salmonella ELISA Export into Qualiproof data base (QS) Sarcoptes scabiei var. suis ELISA SVDV (Virus of Swine Vesicular Disease) ELISA TGEV (Virus of Transmissible Gastroenteritis) ELISA Yersinia (pathogenic) ELISA App ELISA serotypes 5a/b App ELISA serotype 10 App ELISA serotype 12 Brucella RBT Chlamydia CFT CSFV (Virus of Classical or European Swine Fever) ELISA Erysipelothrix rhusiopathiae ( Erysipelas ) ELISA Hps (Haemophilus parasuis) ELISA Influenza A Virus (IAV) ELISA Influenza A Virus (IAV) OF-ELISA for oral fluids only Influenza A Virus (IAV) or Swine Influenza A Virus (SIV) HI (H1N1, H1N2, H3N2, ph1n1 & ph1n2) (recommended for vaccinated sows, no EDTA!) Lawsonia intracellularis ELISA Leptospira (pathogenic serovars) MAT Serological Screenings Screening for fattening pigs App, PMT, PRRSV, Salmonella Respiratory screening Ahlem : App, M. hyopneu., PRRSV, IAV: ELISA/ HI Respiratory screening Glässer : App, Hps, PRRSV, IAV: ELISA/ HI Reproductive tract screening Ery+Parvo : PRRSV, PPV, Leptospira, Erysipelas Reproductive tract screening Melle : PCV2 (IgM/IgG), PRRSV, Leptospira, IAV: ELISA/ HI Health Check Swine : App, M. hyopneu.,erysipelas, Leptospira, PRRSV, PPV, IAV: ELISA/ HI Important! The material best suited for all serological examinations is serum or blood without anticoagulants. Furthermore, detection of antibodies is also possible in bodily fluids, especially oral fluids. Please call us ( ) or see our website for more information. Mycoplasma hyopneumoniae ELISA M. hyopneumoniae ELISA (alternative) Mycoplasma hyorhinis ELISA PMT (Pasteurella multocida toxin) ELISA PCV2 (Porcine Circovirus 2) IgM/IgG ELISA PCV2 ELISA (titration) PEDV ELISA PPV (Porcine Parvovirus) ELISA Diagnostic request form_swine50_eng_ page 3 of 5

4 PCR tests for specific agents Please specify if a pool of (up to 5) individual samples is to be tested: e.g. pool 1-4, 6-10 or , etc. Please indicate if it is already pooled samples! A. pleuropneumoniae (App) Brachyspira hyodysenteriae Brachyspira pilosicoli Brachyspira spec., B. pilosicoli, B. hampsonii, B. intermedia, B. innocens, B. suanatina and B. murdochii Brachyspira with differentiation of all species above incl. Brachyspira hyodysenteriae Campylobacter sp., C. coli and C. jejuni Nucleotide sequence analysis of a PRRSV PCR product incl. alignment with the reference strain of each genotype (Lelystad or VR-2332 = Ingelvac PRRS MLV (BIV)) Alignment with other PRRSV sequences: Porcilis PRRS strain DV (MSD/Intervet) Unistrain PRRS strain VP-046 bis (Hipra) ReproCyc/Ingelvac Flex PRRS EU strain MSV (Boehringer Ingelheim) 96V198 (Zoetis) Chlamydia Rotavirus group A / C Chlamydia with differentiation of species Coronavirus (TGEV + PEDV) Haemophilus parasuis + potential virulence marker Influenza A Virus (IAV) SIV (Swine Influenza Virus) Subtyping (only subsequent to positive IAV PCR) Lawsonia intracellularis Lawsonia intracellularis quantitative PCR (Only testing of individual samples is useful!) Leptospira (pathogenic serovars) Mycoplasma hyopneumoniae Mycoplasma hyorhinis Mycoplasma hyosynoviae Mycoplasma suis ( Eperythrozoonosis ) Pasteurella multocida toxin A PCV2 PCV2 quantitative PCR (Only testing of individual samples is useful!) Nucleotide sequence analysis of a PCV2 PCR product (orf2) incl. alignment PPV PRRSV EU/NA/HP (European and North American genotype or rather species PRRSV-1 and PRRSV-2 as well as highly pathogenic PRRSV NA variants) PRRSV DV (PRRSV EU vaccine strain) Salmonella spec., Serovar Choleraesuis and Typhimurium Important! If swabs are used for PCR, they have to be dry and free of medium. PCR Screenings (combination of single PCRs) Multiplex PCRs PCR Respiration : App, M. hyopneu., IAV, PRRSV PCR Bakum M. hyopneu., IAV, PCV2, PRRSV PCR Dessau App, M. hyopneu., PCV2, PRRSV PCR Dysentery/Ileitis : Brachyspira hyodysenteriae, Lawsonia intracellularis PCR Enteritis : Brachyspira hyodysenteriae, Lawsonia intracellularis, Salmonella spec. PCR Reproduction : Chlamydia, Leptospira, PCV2, PPV, PRRSV PCR Rota-/Coronavirus Rotavirus group A / C, TGEV, PEDV Multiplex PCR Neumünster : M. hyopneu., M. hyorhinis, IAV, PCMV, PCV2, PRCV, PRRSV Multiplex PCR APP/HPS + vir : App, Hps + potential virulence marker Multiplex PCR Serositis : Hps + potential virulence marker, M. hyorhinis Multiplex PCR Arthritis : Hps, M. hyorhinis, M. hyosynoviae, Diagnostic request form_swine50_eng_ page 4 of 5

5 Bacteriological testing Examination by bacteriological culture in general Examination by bacteriological culture only for: (agents) Asservation of pathogenic isolates Antimicrobial susceptibility testing of pathogenic isolates Agar Diffusion Test Microdilution technique Typing of bacterial isolates App Apx toxin typing (PCR) App Serotyping (PCR) Clostridium difficile Toxin genes A/B and binary toxin genes A/B (PCR) Clostridium perfringens Genotyping and toxin production (PCR & Immunoblot) only PCR Parasitological testing Examination for parasite stages (feces) helminth eggs and coccidiae oocysts (Eimeria spec. and Isospora suis) Cryptosporidium detection (feces) Liver fluke eggs (Fasciola) (feces) Mites et al. Sarcoptes and Demodex mites (deep skin scraping) Diagnostic Profile Diarrhea Suckling pig Pathological testing Rotavirus and Coronavirus (PCR) E. coli and C. perfringens (culture) Detection of parasite (Flotation) Macroscopic examination only Immunoblot Histology with a simple report Escherichia coli Virulence-associated factors incl. Edema disease Haemophilus parasuis Serotyping (PCR) vtaa-ls-pcr Prognosis of the virulence potential of Haemophilus parasuis mcr-1 gene (PCR) (colistin resistance) MRSA Methicillin-Resistant Staphylococcus aureus meca gene and PBP2 expression (PCR & agglutination) Pasteurella multocida toxin Detection of toxa gene (PCR) Porcine Mycoplasma Differentiation of: M. flocculare, M. hyopneumoniae, M. hyosynoviae, M. hyorhinis (PCR) Salmonella Serotyping: Serogroups A-E and F-67 (AGG) Staphylococcus hyicus Exfoliative toxin genes A-D and virulence-associated factor (PCR) Capsular type (cps 1, 2, 7 or 9) and virulence-associated factors (PCR) 16S rrna typing for identification of any bacterial species Immunohistology Histology with a detailed report Actinobacillus pleuropneumoniae Influenza A Virus (IAV) Lawsonia intracellularis Leptospira M. hyopneumoniae PCV2 PRRSV Rotavirus group A Further testing and services on request: e.g. examinations for the international swine trade (incl. Classical Swine Fever, Aujezky s disease, TGE, SVD etc.); provision of characterized, bacterial isolates, etc. For information about suitable specimens for the detection of various agents see our website or call us ( ). On our website you will also find a request form for suitable packaging material for samples. Comments: Diagnostic request form_swine50_eng_ page 5 of 5