Facility and Management
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1 Training Course of Microbial Resources Information Management and Utilization for Developing Countries BSL-3 Laboratory Facility and Management Dr. Yiping Zhu Sep
2 Pathogens and BSL-3 laboratory There are about 10 million microbe species have been identified in the world. Among those, only about 400 microbe species are pathogenic to humans, and a little more than 100 species are high pathogenic, but they can ause very serious infecious diseases. Nowadays, merging and reemerging infecious diseases (SARS, Avian flu, Ebola, Zaka ) are big threat to human socity. We need to learn how to deal with, and how to control the diseases. A BSL-3 or BSL-4 laboratory is required when we handling high pathogenic microorganisms.
3 What does a BSL-3 Lab look like?
4 When you go into a BSL-3 Laboratory Biosafety Cabinet 2 Personal Protection Equipment 3 Biohazard Waste Container 4 Biohazard Warning Signs 5 Access control 6 Directed Air Flow
5 The Sandwich structure of BSL-3 Lab BSL-3 Lab Design Requirements: a. 2 door entry (interlock) b. Solid easily cleanable surfaces (floor, walls, ceiling) c. Coved floors d. HEPA (high efficiency particulate air filter) filtered air supply and exhaust e. Negative pressure and directed air flow f. Sealed penetrations g. Hands-free sink near exit door h. Eyewasher and Shower i. Pass through autoclave j. Sewage reatment system
6 Why build a BSL-3 Laboratory? P3 and 3 Ps: Physical Containment level 3 Lab Protect people Protect samples Protect environment BSL-3 Laboratories are designated for handling high pathogenic microbes that may cause serious or potentially lethal disease through the inhalation route of exposure.
7 What build up a BSL-3 Laboratory? Facility & Equipments Ensure Biosafety Biosafety Management BSL-3 Lab
8 Before start to build a BSL-3 Lab Seismic safety assessment 2
9 When design and build a BSL-3 Lab GB : Laboratories-General requirements for biosafety GB : Architectural and technical code for biosafety laboratories
10 Before start runing a BSL-3 Lab Commissioning and pass the Third Party Inspection Capability accreditation by CNAS China National Accreditation Service for Conformity Assessment Laboratory qualification approval by the Ministry of Health The approval of handling high pathogenic microorganisms by Beijing Municipal Health Bureau
11 When runing a BSL-3 Lab Access control Biosafety Management Biosafety Protects people from dangerous hazards Biosecurity Protects hazards from dangerous people Camera Local police
12 Key points of Biosafety Management 1 Staff 2 Material Biosafety Management system 3 4 Operation Routine work 5 Facility 6 Waste
13 1. Staff management Risk awearness Safe operition skill Emergency response Training Evaluation Quiz Operition test Ability assessment Health surveillance Regular health check Immunization Serum storage Counseling Medical services and first aid
14 Why staff training is primary efficient? Heinrich's Law: that in a workplace, for every accident that causes a major injury, there are 29 accidents that cause minor injuries and 300 accidents that cause no injuries.
15 2. Biohazard materials management Classifications Level of Hazard Risk Group 1 No or low individual and community risk. Risk Group 2 Moderate individual risk, low community risk. Risk Group 3 High individual risk, low community risk. Risk Group 4 High individual and community risk.
16 Handling of biohazard materials Pathogens Handling of biohazard materials Name Risk group Culture Animal trials clinical sample Inactived samples Noninfectious samples Mouse leukemia virus 1 BSL-1 ABSL-1 BSL-1 BSL-1 BSL-1 Hepatitis B virus 2 BSL-2 ABSL-2 BSL-2 BSL-1 BSL-1 High pathogenic avian influenza virus 3 BSL-3 ABSL-3 BSL-2 BSL-1 BSL-1 Ebola virus 4 BSL-4 ABSL-4 BSL-3 BSL-2 BSL-1
17 3. Operation management SOPs Emergency response. Risk assessment. and control Good practice
18 4. Routine work management Supplies Clean Decontamination emergency response Incident report
19 5. Facility equipment management a. Daily inspection b. Regular maintainence c. Annual check and test d. Annual maintinence
20 6. Waste management Waste. segregation a.. contaminated sharps b. solid wastes c. cultures, stocks d. liquid wastes e. animal carcasses Waste. decontamination a.. autoclave b. chemical c. incineration d. monitoring (autoclave tapes, biological indicators)
21 Support, Service, Serious Ensure the Biosafety of Your Research
22 Yiping Zhu ,
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