Sangrokbiolab. 상록바이오랩 Tel Fax

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1 Sangrokbiolab Getting accurate and consistent result, every time running a PCR. Saving funds, by capturing vial particles on PCR tube without need of expensive RNA kits. Saving times, by eliminating the time-consuming sample RNA extraction procedure. Flexible applications, by providing universal PCR protocol to fit different PCR cycling systems. Alfalfa Mosaic Virus (AMV) PCR Kit Apple Mosaic Virus (ApMV) PCR Kit Arabis Mosaic Virus (ArMV) PCR Kit Barley Stripe Mosaic Virus (BSMV) PCR Kit Bean Common Mosaic Virus (BCMV) PCR Kit Bean Pod Mottle Virus (BPMV) PCR Kit Bean Yellow Mosaic Virus (BYMV) PCR Kit Beet Necrotic Yellow Vein Virus (BNYVV) PCR Kit Beet Western Yellows Virus (BWYV) PCR Kit Blueberry Necrotic Shock Virus (BlShV) PCR Kit Carnation Etched Ring Virus (CERV) PCR Kit Carnation Latent Virus (CLV) PCR Kit Carnation Mottle Virus (CarMV) PCR Kit Carnation Ringspot Virus (CRSV) PCR Kit Cauliflower Mosaic Virus (CaMV) PCR Kit Cherry Leaf Roll Virus (CLRV) PCR Kit Chrysanthemum Virus B (CVB) PCR Kit Citrus Tristeza Virus (CTV) PCR Kit Cowpea Mosaic Virus (CPMV) PCR Kit Cymbidium Mosaic Virus (CymMV) PCR Kit Garlic Common Latent Virus (GCLV) PCR Kit Impatiens Necrotic Spot Virus (INSV) PCR Kit Iris Yellow Spot Virus (IYSV) PCR Kit Johnsongrass Mosaic Virus (JGMV) PCR Kit Lettuce Mosaic Virus (LMV) PCR Kit Maize Chlorotic Mottle Virus (MCMV) PCR Kit Maize Dwarf Mosaic Virus (MDMV) PCR Kit Onion Yellow Dwarf Virus (OYDV) PCR Kit Papaya Ringspot Virus (PRSV) PCR Kit Pea Enation Mosaic Virus (PEMV) PCR Kit Peach Rosette Mosaic Virus (PRMV) PCR Kit Peanut Stunt Virus (PSV) PCR Kit Pepper Mottle Virus (PepMoV) PCR Kit Pepino Mosaic Virus (PepMV) PCR Kit Pepper Mild Mottle Virus (PMMoV) PCR Kit Potato Leafroll Virus (PLRV) PCR Kit Potato Virus A (PVA) PCR Kit Potato Virus M (PVM) PCR Kit Potato Virus S (PVS) PCR Kit Potato Virus X (PVX) PCR Kit Potato Virus Y (PVY) PCR Kit Prunus Necrotic Ringspot Virus (PNRSV) PCR Kit Southern Bean Mosaic Virus (SBMV) PCR Kit Soybean Mosaic Virus (SMV) PCR Kit Strawberry Latent Ringspot Virus (SLRSV) PCR Kit Strawberry Mild Yellow Edge Virus (SMYEV) PCR Kit Tobacco Etch Virus (TEV) PCR Kit Tobacco Rattle Virus (TRV) PCR Kit Tobacco Ringspot Virus (TRSV) PCR Kit Tobacco Streak Virus (TSV) PCR Kit Tomato Aspermy Virus (TAV) PCR Kit Tomato Ringspot Virus (ToRSV) PCR Kit Tomato Spotted Wilt Virus (TSWV) PCR Kit Turnip Mosaic Virus (TuMV) PCR Kit Wheat Soil-Borne Mosaic Virus (SBWMV) PCR Kit Wheat Streak Mosaic Virus (WSMV) PCR Kit Zucchini Yellow Mosaic Virus (ZYMV) PCR Kit Others: ELISA reagents, Immunostrip, Buffers

2 INSV Impatiens Necrotic Spot Virus TEV Tobacco Etch Virus SR- V028 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V140 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V028 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V140 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V028 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V140 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V028 M4 Amp Immunocapture RT-PCR Kit 48 SR- V140 M4 Amp Immunocapture RT-PCR Kit 48 SR- V028 M5 Amp Immunocapture RT-PCR Kit 96 SR- V140 M5 Amp Immunocapture RT-PCR Kit 96 SR- V028 M6 Amp Immunocapture RT-PCR Kit 288 SR- V140 M6 Amp Immunocapture RT-PCR Kit 288 SR- V028 M7 Complete Immunocapture RT-PCR Kit 48 SR- V140 M7 Complete Immunocapture RT-PCR Kit 48 SR- V028 M8 Complete Immunocapture RT-PCR Kit 96 SR- V140 M8 Complete Immunocapture RT-PCR Kit 96 SR- V028 M9 Complete Immunocapture RT-PCR Kit 288 SR- V140 M9 Complete Immunocapture RT-PCR Kit 288 PepMV Pepino Mosaic Virus ToRSV Tomato Ringspot Virus SR- V114 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V095 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V114 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V095 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V114 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V095 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V114 M4 Amp Immunocapture RT-PCR Kit 48 SR- V095 M4 Amp Immunocapture RT-PCR Kit 48 SR- V114 M5 Amp Immunocapture RT-PCR Kit 96 SR- V095 M5 Amp Immunocapture RT-PCR Kit 96 SR- V114 M6 Amp Immunocapture RT-PCR Kit 288 SR- V095 M6 Amp Immunocapture RT-PCR Kit 288 SR- V114 M7 Complete Immunocapture RT-PCR Kit 48 SR- V095 M7 Complete Immunocapture RT-PCR Kit 48 SR- V114 M8 Complete Immunocapture RT-PCR Kit 96 SR- V095 M8 Complete Immunocapture RT-PCR Kit 96 SR- V114 M9 Complete Immunocapture RT-PCR Kit 288 SR- V095 M9 Complete Immunocapture RT-PCR Kit 288 PMMoV Pepper Mild Mottle Virus AMV Alfalfa Mosaic Virus SR- V068 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V099 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V068 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V099 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V068 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V099 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V068 M4 Amp Immunocapture RT-PCR Kit 48 SR- V068 M5 Amp Immunocapture RT-PCR Kit 96 ApMV Apple Mosaic Virus SR- V068 M6 Amp Immunocapture RT-PCR Kit 288 Catalog No. Description Test SR- V068 M7 Complete Immunocapture RT-PCR Kit 48 SR- V003 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V068 M8 Complete Immunocapture RT-PCR Kit 96 SR- V003 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V068 M9 Complete Immunocapture RT-PCR Kit 288 SR- V003 M3 Cap Immunocapture Kit for RT-PCR 288 PVX Potato Virus X ArMV Arabis Mosaic Virus SR- V045 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V165 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V045 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V165 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V045 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V165 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V045 M4 Amp Immunocapture RT-PCR Kit 48 SR- V045 M5 Amp Immunocapture RT-PCR Kit 96 BSMV Barley Stripe Mosaic Virus SR- V045 M6 Amp Immunocapture RT-PCR Kit 288 Catalog No. Description Test SR- V045 M7 Complete Immunocapture RT-PCR Kit 48 SR- V193 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V045 M8 Complete Immunocapture RT-PCR Kit 96 SR- V193 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V045 M9 Complete Immunocapture RT-PCR Kit 288 SR- V193 M3 Cap Immunocapture Kit for RT-PCR 288

3 BCMV Bean Common Mosaic Virus CarMV Carnation Mottle Virus SR- V097 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V014 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V097 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V014 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V097 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V014 M3 Cap Immunocapture Kit for RT-PCR 288 BPMV Bean Pod Mottle Virus CRSV Carnation Ringspot Virus SR- V082 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V015 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V082 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V015 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V082 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V015 M3 Cap Immunocapture Kit for RT-PCR 288 BYMV Bean Yellow Mosaic Virus CaMV Cauliflower Mosaic Virus SR- V009 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V016 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V009 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V016 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V009 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V016 M3 Cap Immunocapture Kit for RT-PCR 288 BNYVV Beet Necrotic Yellow Vein Virus CLRV Cherry Leaf Roll Virus SR- V100 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V084 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V100 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V084 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V100 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V084 M3 Cap Immunocapture Kit for RT-PCR 288 BWYV Beet Western Yellows Virus CVB Chrysanthemum Virus B SR- V010 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V017 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V010 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V017 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V010 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V017 M3 Cap Immunocapture Kit for RT-PCR 288 BlShV Blueberry Necrotic Shock Virus CTV Citrus Tristeza Virus SR- V073 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V216 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V073 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V216 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V073 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V216 M3 Cap Immunocapture Kit for RT-PCR 288 CERV Carnation Etched Ring Virus CPMV Cowpea Mosaic Virus SR- V013 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V020 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V013 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V020 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V013 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V020 M3 Cap Immunocapture Kit for RT-PCR 288 CLV Carnation Latent Virus CymMV Cymbidium Mosaic Virus SR- V173 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V024 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V173 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V024 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V173 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V024 M3 Cap Immunocapture Kit for RT-PCR 288

4 CymRSV Cymbidium Ringspot Virus PRSV Papaya Ringspot Virus SR- V025 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V034 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V025 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V034 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V025 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V034 M3 Cap Immunocapture Kit for RT-PCR 288 GCLV Garlic Common Latent Virus PEMV Pea Enation Mosaic Virus SR- V026 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V076 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V026 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V076 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V026 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V076 M3 Cap Immunocapture Kit for RT-PCR 288 IYSV Iris Yellow Spot Virus PRMV Peach Rosette Mosaic Virus SR- V132 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V079 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V132 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V079 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V132 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V079 M3 Cap Immunocapture Kit for RT-PCR 288 JGMV Johnsongrass Mosaic Virus PSV Peanut Stunt Virus SR- V113 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V202 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V113 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V202 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V113 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V202 M3 Cap Immunocapture Kit for RT-PCR 288 LMV Lettuce Mosaic Virus PepMoV Pepper Mottle Virus SR- V174 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V039 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V174 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V039 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V174 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V039 M3 Cap Immunocapture Kit for RT-PCR 288 MCMV Maize Chlorotic Mottle Virus PLRV Potato Leafroll Virus SR- V219 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V071 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V219 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V071 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V219 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V071 M3 Cap Immunocapture Kit for RT-PCR 288 MDMV Maize Dwarf Mosaic Virus PVA Potato Virus A SR- V139 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V042 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V139 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V042 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V139 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V042 M3 Cap Immunocapture Kit for RT-PCR 288 OYDV Onion Yellow Dwarf Virus PVM Potato Virus M SR- V033 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V101 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V033 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V101 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V033 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V101 M3 Cap Immunocapture Kit for RT-PCR 288

5 PVS Potato Virus S TRSV Tobacco Ringspot Virus SR- V043 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V089 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V043 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V089 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V043 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V089 M3 Cap Immunocapture Kit for RT-PCR 288 PVY Potato Virus Y TSV Tobacco Streak Virus SR- V116 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V074 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V116 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V074 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V116 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V074 M3 Cap Immunocapture Kit for RT-PCR 288 PNRSV Prunus Necrotic Ringspot Virus TAV Tomato Aspermy Virus SR- V048 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V059 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V048 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V059 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V048 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V059 M3 Cap Immunocapture Kit for RT-PCR 288 SBMV Bean Southern Mosaic Virus TSWV Tomato Spotted Wilt Virus SR- V053 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V063 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V053 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V063 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V053 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V063 M3 Cap Immunocapture Kit for RT-PCR 288 SMV Soybean Mosaic Virus TuMV Turnip Mosaic Virus SR- V094 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V064 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V094 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V064 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V094 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V064 M3 Cap Immunocapture Kit for RT-PCR 288 SLRSV Strawberry Latent Ringspot Virus SBWMV Wheat Soil-Borne Mosaic Virus SR- V055 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V204 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V055 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V204 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V055 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V204 M3 Cap Immunocapture Kit for RT-PCR 288 SMYEV Strawberry Mild Yellow Edge Virus WSSMV Wheat Spindle Streak Mosaic Virus SR- V070 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V066 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V070 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V066 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V070 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V066 M3 Cap Immunocapture Kit for RT-PCR 288 TRV Tobacco Rattle Virus WSMV Wheat Streak Mosaic Virus SR- V058 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V067 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V058 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V067 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V058 M3 Cap Immunocapture Kit for RT-PCR 288 SR- V067 M3 Cap Immunocapture Kit for RT-PCR 288

6 ZYMV Zucchini Yellow Mosaic Virus Catalog No. Description Test SR- V090 M1 Cap Immunocapture Kit for RT-PCR 48 SR- V090 M2 Cap Immunocapture Kit for RT-PCR 96 SR- V090 M3 Cap Immunocapture Kit for RT-PCR 288 Components of Immunocapture PCR Kits Coated PCR Tubes/Plates Sample Buffer 1x8 PCR strips pre-coated with specific capture polyclonal antibody (PAb) or monoclonal antibody (MAb) PCR sample extraction buffer, in 5x concentrate liquid or in dry powder Washing Buffer Primers Master Mix Enzymes Positive Controls Negative Control Instruction PCR plate washing buffer, in 20x concentrate liquid or in dry powder Forward and reverse PCR or RT-PCR primers, in form of freezing-dried powder Master Mix, including RT-PCR buffer, dntps, Mg++, dh2o and/or Rnasin Reverse transcriptase, Taq polymerase ve control made by virus infected material or by viral cdna template, in freezing-dried powder Negative control made by healthy plant material, in freezing-dried powder Specific test instructions for each of the three immunocapture PCR kits Kit Formats Coated PCR Tubes/Plates Cap Immunocapture Kit Amp Immunocapture Kit Complete Immunocapture Kit Coated PCR Tubes/Plates PCR Sample Buffer PCR tube Washing Buffer PCR or RT-PCR Primers Master Mix Enzymes Positive Controls-virus Positive Controls-cDNA Negative Control Instruction

7 Preparing For the Test Make sure all laboratory equipments and facilities required are ready for the test. Prepare a humid box for incubation steps Prepare Buffers from Powders To prepare the 1x buffers, dissolve the buffer powder into D.H 2 O and add tween-20. For sample buffer, mix the powder with small amount of D.H 2 O into a paste (no clumps) before adding more D.H 2 O. Stir for minutes for dissolving completely and make up to final volume. Prepared 1x working solution can be stored at refrigerator (2-6 o C) for up to 3 months. If you have any questions about preparing and using the buffers. 1X Sample Buffer 1X Washing Buffer Buffer Powder (g) Tween-20 (g) Final Vol. (ml) Prepare Controls Add 1.0 ml of sample extraction buffer into the bottles of lyophilized positive and negative controls and mix by gently inverting the bottles until fully dissolved. The prepared control can be used immediately, or divided into aliquots and stored frozen (-10 to - 40oC). Each aliquot should be sufficient for at least one use. Control aliquots must be kept frozen until just before use. Do not refreeze controls once they have been thawed. Using the Control at the time you run the test, remove one control from storage and allow it to thaw. Add 50 μl of the prepared control to the appropriate control well. Prepare PCR Tubes Remove the PCR strips from foil pouch and seal the rests in the foil pouch with the desiccant. Put the strips in a PCR tube rack and mark the strips in case a strip becomes separate from the rack. Make a copy of the attached recording sheet and create a loading diagram by recording the locations of your samples, controls, and other reagents needed. Prepare Samples Select symptomatic and/or infective tissues for the test. Leaf tissue is often used in ELISA testing. How ever, plant tissues such as stem, sprout, seed, tuber, root and others can also be tested. Single sample is suggested to be used in each test well. In some cases, composites of up to ten leaves per test well can be used to make testing more economical. However, too many plant samples per well can reduce the sensitivity of the test. PCR sample buffer is used as extraction buffer. Grind sample with a mortar and pestle, or other grinding devices such as German Grinder or sample bag. If you are using a mortar and pestle, wash and rinse it thoroughly between samples. If you extract plant sap, dilute the sap into sample extraction buffer at a ratio of 1:10 (sap volume: buffer volume). Or you can grind plant tissue in extraction buffer at a 1:10 ratio (tissue weight: extraction Buffer volume).

8 Immunocapture PCR Procedure Sample Dispensing Following your loading diagram on your recording sheet, dispense 50 μl of prepared sample into sample tubes. Dispense 50 μl of positive control into positive control tubes, and 50 μl of negative control into negative control tubes. PCR Plate Incubation Put the plate inside the humid box and incubate for 2-3 hours at room temperature (21-24 C) or overnight in the refrigerator (4 C). Prepare PCR Reaction mixture Specific PCR primers are include in this kit. Enzymes such as reverse transcriptase and/or Taq polymerase, and a Master Mix including PCR buffer, dntps, Mg++, dh2o and/or RNasin, are needed for preparing the PCR reaction mixture. PCR reaction mixture will be prepared basing your lab protocol. 2 ul of the prepared primer solution is used for each PCR reaction. Prepare the reaction Mixture immediately before use. Washing PCR Plate Wash the plate when the incubation is complete. Push the PCR strip tubes down to the rack and make sure it will not separate from the rack during washing. Hold the rack and use a quick flipping motion to empty the wells into a sink or waste container without mixing the contents among tubes. Wash the plate by immediately filling the tubes with washing buffer, then quickly emptying them again. Repeat 6 to 8 times with the washing buffer. Then, rinse one time with distilled water. To remove trace drop of waster from the tubes after washing, hold the rack upside down and tap firmly on a folded paper towel. Thorough and careful washing is very important for removing all plant inhibitors from the tube. Make sure to completely empty the tube for each washing, and to avoid cross-contamination among the tubes. PCR Reaction mixture Dispensing Dispense μl of prepared PCR reaction mixture per tube to all of the tubes of each assay. PCR Cycling PCR cycling reaction is conducted by using the following thermal profile: 55 C for 20 min, 95 C for 5 min, then cycles of 94 C 30 second, and 55 C 30 second and 72 C 30 second. Result Evaluation PCR amplification product is analyzed by grose gel electrophoresis. Assay results of the samples are evaluated by comparing with the results of positive and negative controls. Test results are valid only if positive control tubes give a positive result and negative control tubes remain negative. BCMV, BNYVV, BYV, CMV, CGMMV, CymMV, INSV, ORSV, PRSV, PepMV, PMMoV, PPV, PVA, PVS, PVV, PVX, PVY, PVY-n, TMV, TNV-st A&D, ToMV, TSWV, TYLCV, TuMV, WMV-2, ZYMV Acidovorax avenae subsp. citrulli(aac), Erwinia amylovora (Ea), Ralstonia solanacearum (Rs), Botrytis alli (Ball), Phytophthora spp.(phyt), Pythium spp.(pyth), Rhizoctonia spp.(rhiz), Spongospora subterranea f. sp. subterranea (Sss), Xanthomonas campestris pv. pelargonii (Xcp), Xanthomonas fragariae (Xfr)