Metanephrines HPLC Methode Urine & Plasma
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1 METANEPHRINES IN URINE EN / OF PLASMA M.B.V. HPLC EN FLUORESCENCENTIE DETECTIE Metanephrines Wat maakt onze Metanephrines reagensset zo uniek en veelzijdig. U kunt kiezen voor: De totale fracties in urine en/of De vrije fracties in urine en/of De vrije fracties in plasma Urine- en plasma controles met alle fractieconcentraties beschikbaar. De urinemethodieken zijn éénvoudig en snel uitvoerbaar. Deze worden het meest toegepast. Bij pathologisch verhoogde waarden worden steeds vaker aanvullend voor nog meer informatie de vrije fracties in plasma bepaald. METANEPHRINES IN URINE Products Product no. Quantity URINE Metanephrines Reagent Determinations Metanephrines Urine Control Normal Level x 2 ml Metanephrines Urine Control High Level x 2 ml In één reagensset voor de analyse van Metanephrines in Urine: Bepalen van de totale en/of vrije fracties Total Metanephrine Total Nor-Metanephrine METANEPHRINES IN PLASMA EN / OF: Free Metanephrine Free Nor-Metanephrine Products Product no. Quantity PLASMA Metanephrines Reagent Determinations Metanephrines Plasma Control Normal Level x 2 ml Metanephrines Plasma Control High Level x 2 ml In één reagensset voor de analyse van Metanephrines in Plasma: Bepalen van de vrije fracties Free Metanephrine Free Nor-Metanephrine METANEPHRINES URINE & PLASMA FEN Pagina 1 van 8
2 METANEPHRINES IN URINE SAMPLE MATERIAL Urine Total Metanephrine, Total Nor-Metanephrine en / of Free Metanephrine, Free Nor-Metanephrine ANALYTICAL CONDITIONS Column : Polaris C-18A, 5 µm packing Injection volume : 20 µl Flowrate : 0.7 ml/min Runtime Cal. Standard : 15 min Temperature : 30 C Runtime Urine Controls : 20 min Detection : Fluorescence Runtime Urine Samples : 30 min : excitation 280 nm : emission 310 nm EXPECTED URINE VALUES FOR ADULTS Total Nor-Metanephrine : < 5.0 µmol / liter, < 300 µmol / mol creatinine Total Metanephrine : < 2.0 µmol / liter, < 120 µmol / mol creatinine Free Nor-Metanephrine : < 0.5 µmol / liter, < 50 µmol / mol creatinine Free Metanephrine : < 0.2 µmol / liter, < 20 µmol / mol creatinine METANEPHRINES IN PLASMA SAMPLE MATERIAL Plasma Free Metanephrine, Free Nor-Metanephrine ANALYTICAL CONDITIONS Column : Polaris C-18A, 5 µm packing Injection volume : 35 µl Flowrate : 0.8 ml/min Runtime Cal. Standard : 30 min Temperature : 30 C Runtime Plasma Controls : 30 min Detection : Fluorescence Runtime Plasma Samples : 30 min : excitation 280 nm : emission 310 nm EXPECTED PLASMA VALUES FOR ADULTS Free Nor-Metanephrine : < 1,2 nmol / liter, < 1200 pmol / liter Free Metanephrine : < 1,0 nmol / liter, < 1000 pmol / liter. METANEPHRINES URINE & PLASMA FEN Pagina 2 van 8
3 Chromatogram Calibrator Metanephrines in Urine -1- CC2-015.CH1 : Calibratie curve Metanephrines in Urine : Nor-Metanephrine = 501 nmol / liter : Metanephrine = 501 nmol / liter METANEPHRINES URINE & PLASMA FEN Pagina 3 van 8
4 Chromatogram Totale Metanephrines in Urine -2- TN-058.CH1 : Total Metanephrines in Urine : Total Nor-Metanephrine = 1,23 µmol / liter : Total Metanephrine = 0,54 µmol / liter METANEPHRINES URINE & PLASMA FEN Pagina 4 van 8
5 Chromatogram Vrije Metanephrines in Urine -3- FN-059.CH1 : Free Metanephrines in Urine : Free Nor- Metanephrine = 0,38 µmol / liter : Free Metanephrine = 0,33 µmol / liter METANEPHRINES URINE & PLASMA FEN Pagina 5 van 8
6 Chromatogram Calibrator Vrije Metanephrines in Plasma -4- CC-013.CH1 : Calibratie curve Free Metanephrines in Plasma : Free Nor-Metanephrine = 5,0 nmol / liter : Free Metanephrine = 5,0 nmol / liter METANEPHRINES URINE & PLASMA FEN Pagina 6 van 8
7 Chromatogram Vrije Metanephrines in Plasma -5- N-017.CH1 : Metanephrines Plasma Control, Normal Level, 2966 : Free Nor-Metanephrine = 0,8 nmol / liter : Free Metanephrine = 0,8 nmol / liter METANEPHRINES URINE & PLASMA FEN Pagina 7 van 8
8 Chromatogram Vrije Metanephrines in Plasma -6- H-014.CH1 : Metanephrines Plasma Control, High Level, 2967 : Free Nor-Metanephrine = 2,7 nmol / liter : Free Metanephrine = 2,3 nmol / liter METANEPHRINES URINE & PLASMA FEN Pagina 8 van 8
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