A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS.

Size: px
Start display at page:

Download "A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS."

Transcription

1 A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS. Martin R Green*, Keith Richardson, John Chipperfield, Nick Tomczyk, Martin Palmer. Waters Corporation, Wilmslow, UK, SK9 4AX OVERVIEW PURPOSE- Investigation of the use of common matrix ions for m/z drift correction in complex mixtures. METHODS- Construction of m/z, RT library from repetitive injections of human urine matrix. Accurate mass measurement of eight forensic toxicology standards spiked into urine using matrix library. Comparison of accurate mass measurement using external lock mass. RESULTS- 1ppm RMS mass accuracy and improved precision compared to external lock mass method. INTRODUCTION Orthogonal acceleration Time-of-Flight (oa ToF), accurate mass measurement is a well-established analytical technique. Compensation for temperature-dependent m/z drift is commonly performed by periodic measurement of internal or external reference peaks introduced using a purpose built interface. In many analyses, analytes of interest are minor components within a complex chemical matrix, the composition of which is largely invariant regardless of sample origin. Many common biological, environmental and food matrices are known. In this paper, a library of m/z and retention time (RT) is constructed for common components of a matrix and used to correct for m/z drift for subsequent analyses. This approach is compared with results from periodic external lock mass correction. TO DOWNLOAD A COPY OF THIS POSTER NOTE, VISIT 1

2 METHODS All experiments were performed in positive ion electrospray ionization on a Waters Xevo G2-S QToF mass spectrometer using an ACQUITY UPLC system. Urine samples from three different male subjects (A, B, C) were collected and diluted by 10x before analysis. An aliquot of urine sample (A) was spiked with an eight component mixture of standard forensic toxicology compounds at a concentration of 25pg/μL. Leucine Enkephalin m/z was used to provide a single point calibration correction at one minute intervals during the chromatographic separation introduced via a separate electropspray nebulizer. ToF data was acquired in MS E mode (alternating low and high collision energy) at 10 spectra / second. ACQUITY UPLC Conditions: Column: Mobile Phase: Gradient: ACQUITY HSS T3 2.1mm x 100mm (1.8μm). A. 100% Water + 5mM Ammonium Formate B. 100% Acetonitrile + 0.1% Formic Acid 87%A with a linear ramp to 50%B at 10 minutes then a linear ramp to 95%B at 11 minutes. Column temperature: 40 C Flow rate: 0.4mL/min Injection volume 10μL RESULTS Chromatographic peaks corresponding to matrix components are often intense and reproducible for a given matrix. Matrix peaks often appear in the same RT and m/z range as targeted analytes and are therefore ideal to use for internal m/z reference. Figure 1 shows the base peak chromatogram (BPI) of matrix components from three distinct types of matrix. Urine was used as the matrix in all further work presented. Figure 1. BPI chromatograms from three common matrices. 2

3 Figure 2 shows BPI chromatograms of the urine samples from the three subjects used in this study. Although differences may be seen there is clearly a great deal of commonality between the main components of this matrix. These peaks are from matrix compounds such as urea, creatinine, uric acid, citrate, host/pathogen DNA, host/ pathogen RNA and amino acids. To validate the method in Figure 3, results from ten repeat injections of Urine A were used to generate a m/z, RT library. Figure 4 shows a plot of m/z vs RT for entries in this library. Approximately 10 of the largest peaks in each 1min RT window were selected. Figure 4. Library containing 147 peaks from sample A Figures 5 and 6 show peaks in Urine samples B and C respectively, which can be matched to the library entries created from Urine A. The plots of m/z error (ppm) vs RT (min) show the high degree of commonality between samples from the three subjects. A more exhaustive library could have been constructed by combining peaks from the three samples and including solvent ions at each retention time. Figure 3 shows a simplified flow diagram of the method of m/z drift correction using matrix ions. Figure 5. m/z error (ppm) vs RT (min) for peaks in Urine B matched to matrix li-brary constructed from analysis of Urine A. 127 peaks matched. Figure 6. m/z error (ppm) vs RT (min) for peaks in Urine C matched to matrix li-brary constructed from analysis of Urine A. 131 peaks matched. Urine A was spiked with an eight component forensic toxicology mixture at 25pg/μL. 10 consecutive analyses of this mixture were performed. An artificially large m/z drift was forced by directing a cooling fan at the system. Analysis included monitoring an internal lock mass at one minute intervals for comparison. Peaks corresponding to those in the library (Figure 4) were identified. Figure 7 shows m/z error (ppm) vs. RT for the identified library peaks in each injection. Figure 8 shows the m/z error (ppm) for the external lock mass during each run. Figure 3. Outline of method used to correct for m/z drift using matrix ions. 3

4 Figure 10 shows the mass error (ppm) vs RT for the eight spiked compounds in each of the ten injections after correction using the library matched matrix peaks. For comparison Figure 11 shows the same data m/z corrected using the periodic external reference peak. Figure 7. Library matched data for 10 repeat injections Urine A with spiked standards Figure 8. External lock mass data for 10 repeat injections Urine A with spiked standards A trend line was calculated for each of the ten sets of library matched peaks. An example trend line is shown in Figure 9. The trend line in Figure 9 was generated by determining the median ppm error value of all data points in a 2 minute RT window. The centre of the window was shifted by 1 minute and the median recalculated to determine consecutive points. A linear extrapolation was used between each point. From this trend line a m/z correction factor was calculated at each RT and used to correct the measured values for the eight spiked compounds. For comparison the same data was corrected using the periodic external lock mass. Figure 10. Plot of mass measurement error (ppm) for eight spiked compounds corrected using matrix ions. Figure 11. Plot of mass measurement error (ppm) for eight spiked compounds corrected using external lock mass. Figure 12 shows a table of RMS mass measurement error in ppm for all eight spiked compounds over the ten injections. Results from both data corrected using identified matrix ions and correction using the external lock mass are presented. Figure 12. RMS mass measurement error (ppm) Figure 9. Example of calculated trend line for library matched data allowing calculation of m/z correction factor at each RT. 4

5 DISCUSSION The results in Figure 12 show an increased mass precision when using matrix ions for mass correction, compared to when using an external reference. This is in part due to the large number and intensity of the matrix ions. Introduction of a more intense external lock mass signal would also have resulted in improved precision. All experiments were preformed in MS E mode resulting in a second, high collision energy (CE) function associated with each data file. No attempt was made to use these data, however adding high CE data to the library would increase the number of library entries significantly. In addition, the m/z range of the matrix peaks at each RT would be increased compared to Figure 4. This would allow more detailed correction of mass calibration, if required. Over the course of the experiments presented no significant shift in RT was observed. However, the method discussed may also be used to compensate for RT time shifts as columns age or due to differences between systems. Although not shown in this study, the matrix library may also include ion mobility (IMS) data. The collision cross sections measured in LC-IMS-MS data have been shown to be highly reproducible. Using m/z, drift time and CCS and sophisticated pattern matching software allows matrix ions to be identified regardless of RT. For example, when measuring analytes from the same matrix but with different chromatographic conditions. CONCLUSION m/z drift correction using matrix ions has been shown to give comparable results to using a periodic external reference. The large number of matrix ions at each RT improves measurement precision allowing sub ppm accuracy. Matrix libraries may be built for many matrix types and transferred between different instruments. The high degree of reproducibility of CCS measurements using ion mobility may be used to add specificity to library identification and compensate for retention time changes. The method described removes the need for external or internal references, simplifying the process of mass measurement Waters Corporation 5

UPLC-MS/MS Analysis of Azole Antifungals in Serum for Clinical Research

UPLC-MS/MS Analysis of Azole Antifungals in Serum for Clinical Research Stephen Balloch and Gareth Hammond Waters Corporation, Wilmslow, UK APPLICATION BENEFITS Analytical selectivity afforded by mass selective detection Wide linear measuring range Simple, inexpensive sample

More information

Profiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry

Profiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry Profiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry Michael McCullagh, 1 Kieran Neeson, 2 and Antonietta Gledhill 1 Waters Corporation,

More information

Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition

Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition Evelyn Goh Waters Pacific, Singapore APPLICATION BENEFITS This method allows for the simultaneous analysis of 12 water-soluble

More information

[ APPLICATION NOTE ] APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS

[ APPLICATION NOTE ] APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS [ APPLICATI TE ] Ion Mobility-enabled Data-dependent Experiments Distinguishing Co-eluting Isomeric Metabolites Using an IMS-QTof Mass Spectrometer Jayne Kirk, 1 Russell Mortishire Smith, 1 Robert Beecher,

More information

Analysis of Testosterone, Androstenedione, and Dehydroepiandrosterone Sulfate in Serum for Clinical Research

Analysis of Testosterone, Androstenedione, and Dehydroepiandrosterone Sulfate in Serum for Clinical Research Analysis of Testosterone, Androstenedione, and Dehydroepiandrosterone Sulfate in Serum for Clinical Research Dominic Foley, Michelle Wills, and Lisa Calton Waters Corporation, Wilmslow, UK APPLICATION

More information

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes Catalin E. Doneanu, Weibin Chen, and Jeffrey R. Mazzeo Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F

More information

John Haselden 1, Gordon Dear 1, Jennifer H. Granger 2, and Robert S. Plumb 2. 1GlaxoSmithKline, Ware, UK; 2 Waters Corporation, Milford, MA, USA

John Haselden 1, Gordon Dear 1, Jennifer H. Granger 2, and Robert S. Plumb 2. 1GlaxoSmithKline, Ware, UK; 2 Waters Corporation, Milford, MA, USA Challenges of Polar Compound Analysis Routine LC/MS-based analyses usually rely on using reversed-phase column chemistries (e.g. C 18, C 8, C 4 ) in their LC methodology. However, one of the shortcomings

More information

Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis

Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS

More information

Lipidomic Analysis by UPLC-QTOF MS

Lipidomic Analysis by UPLC-QTOF MS Lipidomic Analysis by UPLC-QTOF MS Version: 1 Edited by: Oliver Fiehn Summary Reagents and Materials Protocol Summary:Lipidomic analysis by UPLC-QTOF mass spectrometry Reagents and Materials: Reagent/Material

More information

[ APPLICATION NOTE ] High Sensitivity Intact Monoclonal Antibody (mab) HRMS Quantification APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS

[ APPLICATION NOTE ] High Sensitivity Intact Monoclonal Antibody (mab) HRMS Quantification APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS Yun Wang Alelyunas, Henry Shion, Mark Wrona Waters Corporation, Milford, MA, USA APPLICATION BENEFITS mab LC-MS method which enables users to achieve highly sensitive bioanalysis of intact trastuzumab

More information

The Application of UPLC/MS E for the Analysis of Bile Acids in Biological Fluids

The Application of UPLC/MS E for the Analysis of Bile Acids in Biological Fluids The Application of UPLC/MS E for the Analysis of Bile Acids in Biological Fluids Elizabeth J. Want, 1 Muireann Coen, Perrine Masson, Hector C. Keun, Jake T.M. Pearce, Michael D. Reily, Donald Robertson,

More information

A UPLC/MS Approach for the Diagnosis of Inborn Errors of Metabolism

A UPLC/MS Approach for the Diagnosis of Inborn Errors of Metabolism Elizabeth Want, 1 Stephen McDonald 2 1 Imperial College, London, UK 2 Waters Corporation, Milford, MA, USA A P P L I C AT ION B E N E F I T S UPLC /MS E was used to profile diagnostic metabolites in a

More information

Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF

Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF PUSHER TOF DETECTOR ZSPRAY TM Ion Source SAMPLING CONE SKIMMER RF HEXAPOLE RF HEXAPOLE QUADRUPOLE IN NARROW BANDPASS MODE

More information

INTRODUCTION CH 3 CH CH 3 3. C 37 H 48 N 6 O 5 S 2, molecular weight Figure 1. The Xevo QTof MS System.

INTRODUCTION CH 3 CH CH 3 3. C 37 H 48 N 6 O 5 S 2, molecular weight Figure 1. The Xevo QTof MS System. Fast and Sensitive in vitro Metabolism Study of Rate and Routes of Clearance for Ritonavir using UPLC CUPLED with the Xevo QTof MS System Jose Castro-Perez, Kate Yu, John Shockcor, Henry Shion, Emma Marsden-Edwards,

More information

Determination of Amantadine Residues in Chicken by LCMS-8040

Determination of Amantadine Residues in Chicken by LCMS-8040 Liquid Chromatography Mass Spectrometry Determination of Amantadine Residues in Chicken by LCMS-8040 A method for the determination of amantadine in chicken was established using Shimadzu Triple Quadrupole

More information

Rapid, Simple Impurity Characterization with the Xevo TQ Mass Spectrometer

Rapid, Simple Impurity Characterization with the Xevo TQ Mass Spectrometer Robert Plumb, Michael D. Jones, and Marian Twohig Waters Corporation, Milford, MA, USA INTRODUCTION The detection and characterization of impurities and degradation products of an active pharmaceutical

More information

High-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring Buprenorphine and Norbuprenorphine in Urine

High-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring Buprenorphine and Norbuprenorphine in Urine High-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring and in Urine Xiaolei Xie, Joe DiBussolo, Marta Kozak; Thermo Fisher Scientific, San Jose, CA Application Note 627 Key Words, norbuprenorphine,

More information

High resolution mass spectrometry for bioanalysis at Janssen. Current experiences and future perspectives

High resolution mass spectrometry for bioanalysis at Janssen. Current experiences and future perspectives High resolution mass spectrometry for bioanalysis at Janssen. Current experiences and future perspectives Lieve Dillen Drug Safety Sciences Analytical Sciences, Non-regulated Bioanalysis Presentation outline

More information

Analysis of drugs of abuse in biological matrix using Time of Flight technology

Analysis of drugs of abuse in biological matrix using Time of Flight technology Analysis of drugs of abuse in biological matrix using Time of Flight technology Niclas Stephanson, PhD Department of Clinical Pharmacology Karolinska University Hospital Karolinska Institutet Stockholm,

More information

Rapid Lipid Profiling of Serum by Reverse Phase UPLC-Tandem Quadrupole MS

Rapid Lipid Profiling of Serum by Reverse Phase UPLC-Tandem Quadrupole MS Rapid Lipid Profiling of Serum by Reverse Phase UPLC-Tandem Quadrupole MS Mark Ritchie and Evelyn Goh Waters Pacific Pte Ltd., Singapore A P P L I C AT ION B E N E F I T S Delivers a rapid 10-min MRM method

More information

4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column

4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column Clinical, Forensic & Toxicology Applications 4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column By Amanda Rigdon Abstract A rapid, sensitive method

More information

SWATH Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids

SWATH Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids SWATH Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids Data Independent Acquisition on TripleTOF and X-Series QTOF Systems for Seized Drug Analysis Oscar G. Cabrices

More information

[ APPLICATION NOTE ] Oasis PRiME HLB Cartridge for Cleanup of Infant Formula Extracts Prior to UPLC-MS/MS Multiresidue Veterinary Drugs Analysis

[ APPLICATION NOTE ] Oasis PRiME HLB Cartridge for Cleanup of Infant Formula Extracts Prior to UPLC-MS/MS Multiresidue Veterinary Drugs Analysis Prior to UPLC-MS/MS Multiresidue Veterinary Drugs Analysis Michael S Young and Kim Van Tran Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Efficient, timesaving multiclass/ multiresidue methodology

More information

Fast Separation of Triacylglycerols in Oils using UltraPerformance Convergence Chromatography (UPC 2 )

Fast Separation of Triacylglycerols in Oils using UltraPerformance Convergence Chromatography (UPC 2 ) Fast Separation of Triacylglycerols in Oils using UltraPerformance Convergence Chromatography (UPC 2 ) Mehdi Ashraf-Khorassani, 1 Larry T. Taylor, 1 Jinchuan Yang, 2 and Giorgis Isaac 2 1 Department of

More information

Development of a Bioanalytical Method for Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid

Development of a Bioanalytical Method for Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid Development of a Bioanalytical Method for Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid Joanne ( 乔安妮 ) Mather Senior Scientist Waters Corporation Data courtesy of Erin Chambers and Mary

More information

Matrix Factor Determination with the Waters Regulated Bioanalysis System Solution

Matrix Factor Determination with the Waters Regulated Bioanalysis System Solution Matrix Factor Determination with the Waters Regulated Bioanalysis System Solution Joanne Mather, Steve Cubbedge, Debadeep Bhattacharya, and Robert S. Plumb Waters Corporation, Milford, MA, U.S. A P P L

More information

High-Throughput Quantitative LC-MS/MS Analysis of 6 Opiates and 14 Benzodiazepines in Urine

High-Throughput Quantitative LC-MS/MS Analysis of 6 Opiates and 14 Benzodiazepines in Urine High-Throughput Quantitative LC-MS/MS Analysis of and 14 Benzodiazepines in Urine Bill Yu, Kristine Van Natta, Marta Kozak, Thermo Fisher Scientific, San Jose, CA Application Note 588 Key Words Opiates,

More information

SYNAPT G2-S High Definition MS (HDMS) System

SYNAPT G2-S High Definition MS (HDMS) System SYNAPT G2-S High Definition MS (HDMS) System High performance, versatility, and workflow efficiency of your MS system all play a crucial role in your ability to successfully reach your scientific and business

More information

LCMS-8030 Application Report. Steroids: Testosterone, Progesterone, Estradiol, Ethinylestradiol

LCMS-8030 Application Report. Steroids: Testosterone, Progesterone, Estradiol, Ethinylestradiol LCMS-8030 Application Report Steroids: Testosterone, Progesterone, Estradiol, Ethinylestradiol Summary: Sensitivity was tested for four steroids on the LCMS-8030 triple quadrupole mass spectrometer coupled

More information

Neosolaniol. [Methods listed in the Feed Analysis Standards]

Neosolaniol. [Methods listed in the Feed Analysis Standards] Neosolaniol [Methods listed in the Feed Analysis Standards] 1 Simultaneous analysis of mycotoxins by liquid chromatography/ tandem mass spectrometry [Feed Analysis Standards, Chapter 5, Section 1 9.1 ]

More information

Quantitative Analysis of -Hydroxybutyrate in Hair Using Target Analyte Finding Processing of Comprehensive GC-HRT Data

Quantitative Analysis of -Hydroxybutyrate in Hair Using Target Analyte Finding Processing of Comprehensive GC-HRT Data Quantitative Analysis of -Hydroxybutyrate in Hair Using Target Analyte Finding Processing of Comprehensive GC-HRT Data LECO Corporation; Saint Joseph, Michigan USA Key Words: Pegasus GC-HRT, GHB, Hair,

More information

Dr. Erin E. Chambers Waters Corporation. Presented by Dr. Diego Rodriguez Cabaleiro Waters Europe Waters Corporation 1

Dr. Erin E. Chambers Waters Corporation. Presented by Dr. Diego Rodriguez Cabaleiro Waters Europe Waters Corporation 1 Development of an SPE-LC/MS/MS Assay for the Simultaneous Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid in Support of Alzheimer s Research Dr. Erin E. Chambers Waters Corporation Presented

More information

O O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions

O O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions Simplifying Qual/Quan Analysis in Discovery DMPK using UPLC and Xevo TQ MS Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION The determination of the drug metabolism

More information

[ APPLICATION NOTE ] UPLC-MS/MS Analysis of 45 Amino Acids Using the Kairos Amino Acid Kit for Biomedical Research INTRODUCTION APPLICATION BENEFITS

[ APPLICATION NOTE ] UPLC-MS/MS Analysis of 45 Amino Acids Using the Kairos Amino Acid Kit for Biomedical Research INTRODUCTION APPLICATION BENEFITS UPLC-MS/MS Analysis of 45 Amino Acids Using the Kairos Amino Acid Kit for Biomedical Research Padhraic Rossiter, 1 Jaime Salcedo Dominguez, 1 Jennifer Warren, 1 Norma Breen, 1 Lisa Calton 2 1 Waters Corporation,

More information

Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection. EPL-BAS Method No.

Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection. EPL-BAS Method No. Page 1 of 10 Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection EPL-BAS Method No. 205G881B Method Summary: Residues of 6-CPA are

More information

Rapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases

Rapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases Clinical, Forensic & Toxicology Applications Rapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases By Shun-Hsin

More information

PosterReprint OVERVIEW

PosterReprint OVERVIEW OVERVIEW A rapid multi-residue GC/MS method was developed for pesticides in foodstuffs. Plum extract was used as an example matrix. Analysis time was reduced from minutes to minutes. pesticides were analysed

More information

Dienes Derivatization MaxSpec Kit

Dienes Derivatization MaxSpec Kit Dienes Derivatization MaxSpec Kit Item No. 601510 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION

More information

Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE. PLD+ Prior to LC-MS/MS Analysis

Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE. PLD+ Prior to LC-MS/MS Analysis Application Note AN842.V. Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE PLD+ Prior to LC-MS/MS Analysis Page Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE PLD+ Prior to LC-MS/MS

More information

Analysis of Food Sugars in Various Matrices Using UPLC with Refractive Index (RI) Detection

Analysis of Food Sugars in Various Matrices Using UPLC with Refractive Index (RI) Detection Analysis of Food Sugars in Various Matrices Using UPLC with Refractive Index (RI) Detection Mark E. Benvenuti Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Using UPLC with RI detection enables

More information

Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations

Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations For Research Use Only. Not for use in diagnostic procedures. 1 2015 AB Sciex RUO-MKT-11-1018-A For research

More information

A Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS

A Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS A Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS By Shun-Hsin Liang and Frances Carroll Abstract Vitamin K₁ and K₂ analysis is typically complex and time-consuming because these

More information

Comprehensive Forensic Toxicology Screening in Serum using On-Line SPE LC-MS/MS

Comprehensive Forensic Toxicology Screening in Serum using On-Line SPE LC-MS/MS Comprehensive Forensic Toxicology Screening in Serum using On-Line SPE LC-MS/MS SCIEX QTRAP 4500 LC-MS/MS System and Spark Holland PICO Adrian M. Taylor 1, Peter Ringeling 2, Martin Sibum 2, Stefan Sturm

More information

A Simple and Accurate Method for the Rapid Quantitation of Drugs of Abuse in Urine Using Liquid Chromatography

A Simple and Accurate Method for the Rapid Quantitation of Drugs of Abuse in Urine Using Liquid Chromatography Application Note LCMS-109 A Simple and Accurate Method for the Rapid Quantitation of Drugs of Abuse in Urine Using Liquid Chromatography Time of Flight (LC-TOF) Mass Spectrometry Introduction Many clinical

More information

[Application Note] TOXICOLOGY SCREENING BY UPLC/PDA IN COMBINATION WITH AN EXTENSIVE COMPOUND LIBRARY

[Application Note] TOXICOLOGY SCREENING BY UPLC/PDA IN COMBINATION WITH AN EXTENSIVE COMPOUND LIBRARY TOXICOLOGY SCREENING BY UPLC/PDA IN COMBINATION WITH AN EXTENSIVE COMPOUND LIBRARY Camille Chatenay, 1,2 Fabien Bévalot, 1,2 Cyril Mounier, 1 Jean Michel Prévosto 1 1 Hôpital d Instruction des Armées Desgenettes,

More information

A Metabolomics Approach to Profile Novel Chemical Markers for Identification and Authentification of Terminalia Species

A Metabolomics Approach to Profile Novel Chemical Markers for Identification and Authentification of Terminalia Species A Metabolomics Approach to Profile Novel Chemical Markers for Identification and Authentification of Terminalia Species Bharathi Avula, 1 Kate Yu, 2 Yan-Hong Wang, 1 Jordan Blodgett, 2 Alan Millar, 2 and

More information

Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification

Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification 22 SEPARATION SCIENCE REDEFINED MAY 2005 Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification In the drug discovery process the detection and

More information

Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID

Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID James Little, Eastman Chemical Company, Kingsport, TN Overview Phospholipids and

More information

Meeting Challenging Requirements for the Quantitation of Regulated Growth Promoters Dexamethasone and Betamethasone in Liver and Milk

Meeting Challenging Requirements for the Quantitation of Regulated Growth Promoters Dexamethasone and Betamethasone in Liver and Milk Meeting Challenging Requirements for the Quantitation of Regulated Growth Promoters Dexamethasone and Betamethasone in Liver and Milk Yoann Deceuninck 1, Emmanuelle Bichon 1, Paul Silcock 2, Fabrice Monteau

More information

Quantitative Analysis of THC and Main Metabolites in Whole Blood Using Tandem Mass Spectrometry and Automated Online Sample Preparation

Quantitative Analysis of THC and Main Metabolites in Whole Blood Using Tandem Mass Spectrometry and Automated Online Sample Preparation Quantitative Analysis of THC and Main Metabolites in Whole Blood Using Tandem Mass Spectrometry and Automated Online Sample Preparation Valérie Thibert, Bénédicte Duretz Thermo Fisher Scientific, Courtaboeuf,

More information

CHARACTERIZATION AND DETECTION OF OLIVE OIL ADULTERATIONS USING CHEMOMETRICS

CHARACTERIZATION AND DETECTION OF OLIVE OIL ADULTERATIONS USING CHEMOMETRICS CHARACTERIZATION AND DETECTION OF OLIVE OIL ADULTERATIONS USING CHEMOMETRICS Paul Silcock and Diana Uria Waters Corporation, Manchester, UK AIM To demonstrate the use of UPLC -TOF to characterize and detect

More information

ApplicationNOTE EXACT MASS MEASUREMENT OF ACTIVE COMPONENTS OF TRADITIONAL HERBAL MEDICINES BY ORTHOGONAL ACCELERATION TIME-OF-FLIGHT.

ApplicationNOTE EXACT MASS MEASUREMENT OF ACTIVE COMPONENTS OF TRADITIONAL HERBAL MEDICINES BY ORTHOGONAL ACCELERATION TIME-OF-FLIGHT. Objective The aim of the study presented is to determine the presence of target Gingkolides in Gingko biloba leaf extract. The data presented shows Gingkolides determined to be present in the extract analyzed.

More information

Dry eye disease commonly known as atopic keratoconjunctivitis is an autoimmune disease of

Dry eye disease commonly known as atopic keratoconjunctivitis is an autoimmune disease of 4.1. Introduction Dry eye disease commonly known as atopic keratoconjunctivitis is an autoimmune disease of eyes. The disease is characterized by lesser or some time no-significant production of tear;

More information

ION MOBILITY COUPLED TO HIGH RESOLUTION MASS SPECTROMETRY: THE POSSIBILITIES, THE LIMITATIONS

ION MOBILITY COUPLED TO HIGH RESOLUTION MASS SPECTROMETRY: THE POSSIBILITIES, THE LIMITATIONS ION MOBILITY COUPLED TO HIGH RESOLUTION MASS SPECTROMETRY: THE POSSIBILITIES, THE LIMITATIONS Anton Kaufmann Official Food Control Authority of the Canton of Zurich (Kantonales Labor Zürich) Switzerland

More information

Characterization of an Unknown Compound Using the LTQ Orbitrap

Characterization of an Unknown Compound Using the LTQ Orbitrap Characterization of an Unknown Compound Using the LTQ rbitrap Donald Daley, Russell Scammell, Argenta Discovery Limited, 8/9 Spire Green Centre, Flex Meadow, Harlow, Essex, CM19 5TR, UK bjectives unknown

More information

Application Note. Abstract. Authors. Pharmaceutical

Application Note. Abstract. Authors. Pharmaceutical Analysis of xycodone and Its Metabolites-oroxycodone, xymorphone, and oroxymorphone in Plasma by LC/MS with an Agilent ZRBAX StableBond SB-C18 LC Column Application ote Pharmaceutical Authors Linda L.

More information

Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur

Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS Kayleigh Arthur K.Arthur@lboro.ac.uk Introduction LC-MS is a highly used technique for untargeted profiling analyses

More information

International Journal of Applied Pharmaceutical Sciences and Research

International Journal of Applied Pharmaceutical Sciences and Research International Journal of Applied Pharmaceutical Sciences and Research 2017; 2(3): 55-63 Anas et al/international Journal of Applied Pharmaceutical Sciences and Research 2017; 2(3): 55-63 International

More information

Quantitative Analysis of Opiates in Urine Using RRHT LC/MS/MS. Application. Authors. Introduction. Abstract. Forensics

Quantitative Analysis of Opiates in Urine Using RRHT LC/MS/MS. Application. Authors. Introduction. Abstract. Forensics Quantitative Analysis of piates in Urine Using RRHT LC/MS/MS Application Forensics Authors Sheher Mohsin Agilent Technologies, Inc. 10 N. Martingale Rd., Suite 550 Schaumburg, IL 60173 USA Yanan Yang Agilent

More information

Quantification of Budesonide Using UPLC and Xevo TQ-S

Quantification of Budesonide Using UPLC and Xevo TQ-S Tirupateswara Rao B., Sudarshan Mantha, and Gopal Vaidyanathan Waters India MS Application Laboratory, Bangalore, India A P P L I C AT ION B E N E F I T S This work demonstrates the benefits of the Regulated

More information

PosterREPRINT RAPID, SELECTIVE SCREENING OF URINE SAMPLES FOR GLUCURONIDES BY LC/MS/MS INTRODUCTION ABSTRACT

PosterREPRINT RAPID, SELECTIVE SCREENING OF URINE SAMPLES FOR GLUCURONIDES BY LC/MS/MS INTRODUCTION ABSTRACT ABSTRACT The traditional study of in vivo drug metabolism in plasma or urine samples is often complicated by the presence of many endogenous compounds. Several mass spectrometric techniques are often applied

More information

Analyze Barbiturates in Urine with Agilent 6430 LC/MS/MS and Poroshell 120 EC-C18

Analyze Barbiturates in Urine with Agilent 6430 LC/MS/MS and Poroshell 120 EC-C18 Analyze Barbiturates in Urine with Agilent 6 LC/MS/MS and Poroshell EC-C8 Application ote Forensic Toxicology Authors Elijah Steinbauer and Pat Friel Toxicology Laboratory at the Veterans Administration

More information

Direct Analysis of Folic Acid in Digestive Juices by LC/TOF-MS Application

Direct Analysis of Folic Acid in Digestive Juices by LC/TOF-MS Application Direct Analysis of Folic Acid in Digestive Juices by LC/TOF-MS Application Food Safety Authors M. H. Blokland, P. W. Zoontjes, S. S. Sterk, and L. A. van Ginkel, National Institute of Public Health and

More information

Direct Analysis of Urinary Opioids and Metabolites by Mixed-Mode µelution SPE Combined with UPLC/MS/MS for Forensic Toxicology

Direct Analysis of Urinary Opioids and Metabolites by Mixed-Mode µelution SPE Combined with UPLC/MS/MS for Forensic Toxicology Direct Analysis of Urinary Opioids and Metabolites by Mixed-Mode µelution SPE Combined with UPLC/MS/MS for Forensic Toxicology Jonathan P. Danaceau, Erin E. Chambers, and Kenneth J. Fountain Waters Corporation,

More information

Detection of Cotinine and 3- hydroxycotine in Smokers Urine

Detection of Cotinine and 3- hydroxycotine in Smokers Urine Detection of Cotinine and 3- hydroxycotine in Smokers Urine Behavioural and Situational Research Group School of Medicine, University of Tasmania Version number: 2 Effective date: 01/12/2015 Review due:

More information

MS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine

MS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine Application Note: 346 MS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine Gargi Choudhary and Diane Cho, Thermo Fisher Scientific, San Jose, CA Wayne Skinner and

More information

Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222

Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Joan Stevens, Ph.D.; Luke Roenneburg; Tim Hegeman; Kevin Fawcett

More information

Profiling Analysis of Polysulfide Silane Coupling Agent

Profiling Analysis of Polysulfide Silane Coupling Agent C146-E154 Profiling Analysis of Polysulfide lane Coupling Agent Technical Report vol.42 1. Introduction Recently, in line with the oil-conservation movement, silica, as a non-petroleum resource, is increasingly

More information

Using Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies.

Using Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies. Using Software Tools to Improve the Detection of Impurities Application Note Christine Miller Introduction The analysis of raw materials and finished products for or impurities presents a challenge in

More information

Quantitative Analysis of Drugs of Abuse in Urine using UHPLC Coupled to Accurate Mass AxION 2 TOF Mass Spectrometer

Quantitative Analysis of Drugs of Abuse in Urine using UHPLC Coupled to Accurate Mass AxION 2 TOF Mass Spectrometer application Note Liquid Chromatography/ Mass Spectrometry Authors Sharanya Reddy Blas Cerda PerkinElmer, Inc. Shelton, CT USA Quantitative Analysis of Drugs of Abuse in Urine using UHPLC Coupled to Accurate

More information

[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector

[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Mark Benvenuti, Gareth Cleland, and Jennifer Burgess Waters Corporation, Milford, MA, USA

More information

Chemical Analysis Business Operations Waters Corporation Milford MA

Chemical Analysis Business Operations Waters Corporation Milford MA The Detection and Identification of Unknown Contaminants During ToF Screening and Structural Elucidation for Pesticides in River Water Using an Integrated Software Approach Chemical Analysis Business Operations

More information

Ultra High Definition Optimizing all Analytical Dimensions

Ultra High Definition Optimizing all Analytical Dimensions Ultra High Definition Optimizing all Analytical Dimensions Sensitivity Dynamic Range Signal Response Linearity Separation Speed Peak Capacity Chromatogram Mass Spectrum Mass Accuracy Resolving Power Acquisition

More information

Fast, Robust and Reliable Method for the Identification and Quantitation of Sildenafil Residue in Honey using LC-MS/MS

Fast, Robust and Reliable Method for the Identification and Quantitation of Sildenafil Residue in Honey using LC-MS/MS Fast, Robust and Reliable Method for the Identification and Quantitation of Sildenafil Residue in Honey using LC-MS/MS Praveen K. Sharma 1, Neha Bhasin 1, Prasanth Joseph 1, Manoj G Pillai 1, and André

More information

Vitamin D3 and related compounds by ESI and APCI

Vitamin D3 and related compounds by ESI and APCI Liquid Chromatography Mass Spectrometry SSI-LCMS-9 Vitamin D and related compounds by ESI and APCI LCMS-8 Summary Vitamin D and related compounds were measured by LC-ESI/APCI-MS-MS. Background Accurate

More information

Amphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 6430 LC/MS/MS

Amphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 6430 LC/MS/MS Amphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 643 LC/MS/MS Application Note Forensics Authors Jason Hudson, Ph.D., James Hutchings, Ph.D., and Rebecca Wagner, Ph.D. Virginia

More information

Application Note LCMS-108 Quantitation of benzodiazepines and Z-drugs in serum with the EVOQ TM LC triple quadrupole mass spectrometer

Application Note LCMS-108 Quantitation of benzodiazepines and Z-drugs in serum with the EVOQ TM LC triple quadrupole mass spectrometer Application Note LCMS-108 Quantitation of benzodiazepines and Z-drugs in serum with the EVOQ TM LC triple quadrupole mass spectrometer Abstract This study demonstrates a sensitive, rapid and reliable research

More information

application Natural Food Colorants Analysis of Natural Food Colorants by Electrospray and Atmospheric Pressure Chemical Ionization LC/MS

application Natural Food Colorants Analysis of Natural Food Colorants by Electrospray and Atmospheric Pressure Chemical Ionization LC/MS application LC/MS Natural Food Colorants Analysis of Natural Food Colorants by Electrospray and Atmospheric Pressure Chemical Ionization LC/MS Introduction Many kinds of natural colors are used in beverages,

More information

SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine

SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 709 Key Words SPE, SOLA

More information

Workflow for Screening and Quantification of the SAMHSA (NIDA) Panel in Urine Using UHPLC-TOF

Workflow for Screening and Quantification of the SAMHSA (NIDA) Panel in Urine Using UHPLC-TOF APPLICATIO OTE Liquid Chromatography/ Mass Spectrometry Authors: Avinash Dalmia Joanne Mather PerkinElmer, Inc. Shelton, CT Workflow for Screening and Quantification of the SAMHSA (IDA) Panel in Urine

More information

SEPARATION OF BRANCHED PFOS ISOMERS BY UPLC WITH MS/MS DETECTION

SEPARATION OF BRANCHED PFOS ISOMERS BY UPLC WITH MS/MS DETECTION SEPARATION OF BRANCHED PFOS ISOMERS BY UPLC WITH MS/MS DETECTION Marian Twohig 1, Nicholas Ellor 1, Keith Worrall 2, Tim Jenkins 2, Gordon Kearney 2 1 Waters Corporation, 1 Cummings Center, Beverly, MA

More information

Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system

Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 9 Infinity II/63B LC/TOF system Application Brief Authors Mike Adams, Karen Kaikaris, and A. Roth CWC Labs Joan Stevens, Sue D

More information

A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids

A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids Jeremy Netto, 1 Stephen Wong, 1 Federico Torta, 2 Pradeep Narayanaswamy, 2

More information

Profiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography

Profiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography Profiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography Application Note Metabolomics Author Yuqin Dai Agilent Technologies, Inc. 31 Stevens Creek Blvd,

More information

Method Development for the Analysis of Endogenous Steroids Using Convergence Chromatography with Mass Spectrometric Detection

Method Development for the Analysis of Endogenous Steroids Using Convergence Chromatography with Mass Spectrometric Detection Method Development for the Analysis of Endogenous Steroids Using Convergence Chromatography with Mass Spectrometric Detection Christopher J. Hudalla, Stuart Chadwick, Fiona Liddicoat, Andrew Peck, and

More information

Sample Preparation is Key

Sample Preparation is Key PLOS ONE DOI: 10.1371/journal.pone.0117232 February 6, 2015 Presented by Katie Gibbs Sample Preparation is Key Sample extraction and instrumental analysis methods are well documented in metabolomics. Understanding

More information

Rapid Analysis of Bisphenols A, B, and E in Baby Food and Infant Formula Using ACQUITY UPLC with the Xevo TQD

Rapid Analysis of Bisphenols A, B, and E in Baby Food and Infant Formula Using ACQUITY UPLC with the Xevo TQD Rapid Analysis of Bisphenols A, B, and E in Baby Food and Infant Formula Using ACQUITY UPLC with the Xevo TQD Claude R. Mallet Waters Corporation, Milford, MA, USA A P P L I C AT ION B E N E F I T S This

More information

Quantitation of Four Regulated Phthalates in Plastic Consumer Products by Gas Chromatography Time-of-Flight Mass Spectrometry

Quantitation of Four Regulated Phthalates in Plastic Consumer Products by Gas Chromatography Time-of-Flight Mass Spectrometry Quantitation of Four Regulated Phthalates in Plastic Consumer Products by Gas Chromatography Time-of-Flight Mass Spectrometry LEC Corporation; Saint Joseph, Michigan USA Key Words: Phthalates, GC/MS, GC-MS,

More information

Fully Automated Online Sample Preparation AND Quantification of Amiodarone from Whole Blood using CLAM-LC-MS/MS

Fully Automated Online Sample Preparation AND Quantification of Amiodarone from Whole Blood using CLAM-LC-MS/MS PO-CON177E Fully Automated Online Sample Preparation AND Quantification of Amiodarone from Whole Blood using CLAM-LC-MS/MS ASMS 17 ThP 93 Christian Bunse 1, Lars Kröner 1 Shimadzu Deutschland GmbH, Duisburg,

More information

Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform

Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Abstract Targeted proteomics for biomarker verification/validation

More information

Analysis of Rosuvastatin in Dried Blood Spot and Plasma Using ACQUITY UPLC with 2D Technology

Analysis of Rosuvastatin in Dried Blood Spot and Plasma Using ACQUITY UPLC with 2D Technology Analysis of Rosuvastatin in Dried Blood Spot and Plasma Using ACQUITY UPLC with 2D Technology Claude Mallet, 1 Jennifer Simeone, 2 Paul Rainville 3 1 Workflow Integration Group, Separations Technologies,

More information

Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma Using μelution SPE and a CORTECS UPLC Column

Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma Using μelution SPE and a CORTECS UPLC Column Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma Using μelution SPE and a CORTECS UPLC Column Erin E. Chambers and Kenneth J. Fountain Waters Corporation, Milford, MA, USA

More information

Key Advantages of Comprehensive Cannabis Analysis

Key Advantages of Comprehensive Cannabis Analysis Comprehensive Cannabis Analysis: Pesticides, Aflatoxins, Terpenes, and High Linear Dynamic Range Potency from One Extract Using One Column and One Solvent System Robert Di Lorenzo 1, Diana Tran 2, KC Hyland

More information

Jose Castro-Perez, Henry Shion, Kate Yu, John Shockcor, Emma Marsden-Edwards, Jeff Goshawk Waters Corporation, Milford, MA, U.S. and Manchester, UK

Jose Castro-Perez, Henry Shion, Kate Yu, John Shockcor, Emma Marsden-Edwards, Jeff Goshawk Waters Corporation, Milford, MA, U.S. and Manchester, UK HIGH-THRUGHPUT REACTIVE METABLITE SCREEIG FR DICLFEAC BY UPLC AD XEV TQ MS WITH SCAWAVE Jose Castro-Perez, Henry Shion, Kate Yu, John Shockcor, Emma Marsden-Edwards, Jeff Goshawk Waters Corporation, Milford,

More information

Low-level environmental phthalate exposure associates with urine metabolome. alteration in a Chinese male cohort

Low-level environmental phthalate exposure associates with urine metabolome. alteration in a Chinese male cohort Supporting Information Low-level environmental phthalate exposure associates with urine metabolome alteration in a Chinese male cohort Jie Zhang*, Liangpo Liu, Xiaofei Wang, Qingyu Huang, Meiping Tian,

More information

Latest Innovations in LC/MS/MS from Waters for Metabolism and Bioanalytical Applications

Latest Innovations in LC/MS/MS from Waters for Metabolism and Bioanalytical Applications Latest Innovations in LC/MS/MS from Waters for Metabolism and Bioanalytical Applications Ignatius J. Kass Senior Field Marketing Manager Pharmaceutical MS Challenges in Pharmaceutical Sample Analysis Quantitative

More information

The Investigation of Factors Contributing to Immunosuppressant Drugs Response Variability in LC-MS/MS Analysis

The Investigation of Factors Contributing to Immunosuppressant Drugs Response Variability in LC-MS/MS Analysis The Investigation of Factors Contributing to Immunosuppressant Drugs Variability in LC-MS/MS Analysis Joseph Herman, Dayana Argoti, and Sarah Fair Thermo Fisher Scientific, Franklin, MA, USA Overview Purpose:

More information

SUPPORTING INFORMATION:

SUPPORTING INFORMATION: 1S SUPPORTING INFORMATION: The discovery of the once weekly glucagon like peptide 1 (GLP-1) analog semaglutide Jesper Lau*, Paw Bloch, Lauge Schäffer, Ingrid Pettersson, Jane Spetzler, Jacob Kofoed, Kjeld

More information

International Journal of Applied Pharmaceutical Sciences and Research

International Journal of Applied Pharmaceutical Sciences and Research International Journal of Applied Pharmaceutical Sciences and Research Research Article http://dx.doi.org/10.21477/ijapsr.v2i03.8254 Analytical Separation and Characterisation of Degradation Products and

More information

Probing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection INTRODUCTION

Probing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection INTRODUCTION Probing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection Michael Jones Waters Corporation, Wilmslow, UK APPLICATION BENEFITS The ACQUITY Arc System is

More information