Session 2016: Sections: Class Location: Days / Time: Instructor:

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Lee Matthews
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1 Student Author Name: Team Members Name(s): Student Author ID #: Team Members ID # (s): Lab Assignment #: 8 Team Name: Dream Team Lab Assignment Title: Blood Glucose Date: Background: In a properly functioning/healthy body, blood glucose levels are appropriately controlled by secretion of insulin. This lab was designed to investigate a group of subjects and how each of their bodies reacted after consuming a food or drink that contained a form of sugar. All the subject s blood glucose levels spiked after consuming a form of sugar except the one subject who only consumed water. Purpose and Objective: The purpose of this lab was to observe how the human body reacts after consuming carbohydrates. Over time, we observed how blood glucose levels spike and came back down over a period of time. Hypotheses: We hypothesize that each subject s blood glucose levels will significantly spike after consuming a form of sugar except the controlled subject who only consumed water. A person with a higher BMI will take longer to bring blood glucose levels back down to normal. Subjects of older age will have a higher blood glucose level right after consuming a form of sugar. Subjects: This table lists each female subject and her glucose dosage/form of sugar, age, and BMI. Page 1 of 14

2 Female Subjects Subject's Initials HL CR SA MS RT AA Glucose Dosage Grams of Candy 10 Grams of Sucrose Water Only 10 Grams of Sucrose 10 Grams of Sucrose 10 Grams of Sucrose and 10 Grams of Gatorade Age BMI This table lists each male subject and his glucose dosage/form of sugar, age, and BMI. Male Subjects Subject's Initials BR DV Glucose Dosage 10 Grams of Sucrose Grams of Candy and Grams of Gatorade Age BMI Materials: List of Disposable Materials Used Diabetic Lancets Blood Glucose Test Strips Hand Sanitizer/Soap Paper Towels Pierced subject s finger to produce blood Used to collect subject s blood for glucose meter To reduce bacteria/germs Dry/wipe hands Procedures: 1) Each subject checked their blood glucose level using a lancet to prick their finger to put on a test strip to insert Page 2 of 14

3 into the blood glucose meter to read what their blood glucose level was. 2) Each subject either ate 10 grams of sucrose, 16 grams of candy or 22 grams of Gatorade. The one diabetic subject drank water. 3) Each subject then checked their blood glucose level after 20 minutes and recorded the level. 4) Each subject then checked their blood glucose level after 40 minutes and recorded the level. Results and Data: Table 1: Consists all of subject s data including averages for each condition. Graph 1: Before, 20 minutes post, and 40 minutes post each subject had their blood glucose level taken. Notice in this graph how one of the subject s stands out. Subject SA had their blood glucose level drop during 20 minutes post glucose dosage while the remaining subject s level rose. Graph 2: A bar graph comparing all the subjects blood glucose level data and the mean. Graph 3: The linear trendline gives a better understanding of what subjects are above or below the average blood glucose level. BR had the highest average and MS had the lowest. Graph 4: A Bar graph shows the mean of every subject s average blood glucose level. The Linear trendline gives a better understanding who is above and below the average blood glucose level. Graph 5: This scatter graph shows the subjects BMI vs. their blood glucose level after 20 minutes. The trendline shows that the higher or lower a subjects BMi is that it does not affect a great amount of the subjects blood glucose level. Graph 6: This scatter graph shows the subjects BMI vs. their blood glucose level after 40 minutes. All subjects blood glucose level did return to normal. The trendline shows again, that BMI does not play a factor in subjects blood glucose level. Graph 7: All non diabetic subjects started at a normal blood glucose level, post 20 minutes all subject s blood glucose spiked and post 40 minutes all subjects blood glucose returned to normal. The one subject who was diabetic started with a normal blood glucose level, post 20 minutes it fell and post 40 minutes spiked a significant amount. Graph 8: The subject started off with a normal blood glucose level. Since the subject is type 1 Diabetic and needs to take insulin after consuming food the subjects blood glucose level decreased. Post 40 minutes the subject s blood glucose level returned to normal due to taking the correct amount of insulin for the food consumed. Graph 9: The non diabetic subjects blood glucose mean is much lower when starting and post 40 minutes, due to the fact their body regulates sugar on its own. The diabetic subject has a higher starting blood glucose level and a highper post 40 minutes due to the fact the subjects body can not regulate sugar on its own. Graph 10: The scatter graph shows the subjects BMI vs their average blood glucose level. Subjects with a higher BMI seem to have a lower Blood glucose level than the subject s with a lower BMI. The linear trend shows the lower the BMI the higher the blood glucose level Graph 11: The bar graph shows age vs. blood glucose level. The bar graph shows being older does affect your blood glucose level. Analysis and Discussion: Non diabetic subjects overall have a better blood glucose level. Non diabetic subjects blood glucose level did exactly what it should do after eating a substance with sugar. The subjects blood glucose level spiked and then returned to their starting blood glucose level. The subjects BMI did not have any affect on their blood glucose level. The subjects age does have an effect on their blood glucose level. The subject who has diabetes has an overall higher blood glucose level. The subject who has diabetes their blood glucose level dropped after they ate due to the fact the subject has to take insulin to counteract the sugar intake. When the subject checked their blood sugar 40 minutes after their blood glucose was back to normal. Page 3 of 14

4 Conclusions / Further Considerations: It was made very clear that being a non diabetic ones body does exactly what it was suppose to do. Eating a sugar substance spikes your blood glucose level. When 40 minutes had passed every non diabetic subjects blood glucose level was back to normal due to the insulin their body produced. The subject who was diabetic it was made clear that does not happen the same way as a non diabetic. The subjects blood glucose level dropped very low due to the fact they had to take insulin after eating. When 40 minutes had passed their blood glucose level was finally back at a normal blood glucose level, but was still higher than all the non diabetics. The subjects age or BMI did not have any effect on their blood glucose level, before, during or after when they took their blood glucose level. Page 4 of 14

5 ATTACHMENTS: Table 1 Graph 1 Page 5 of 14

6 Graph 2 Page 6 of 14

7 Graph 3 Page 7 of 14

8 Graph 4 Page 8 of 14

9 Graph 5 Page 9 of 14

10 Graph 6 Page 10 of 14

11 Graph 7 Page 11 of 14

12 Graph 8 Page 12 of 14

13 Graph 9 Page 13 of 14

14 Graph 10 Page 14 of 14

Test and graph the glucose levels in blood plasma samples collected during the patient s glucose tolerance test.

Test and graph the glucose levels in blood plasma samples collected during the patient s glucose tolerance test. Diagnosing Diabetes: The Case: Your patient reports problems with fatigue and increased urination. You suspect that she might have diabetes. The results of her blood tests indicate that her blood glucose