726 MIYAZAKI A et al. Circ J 2017; 81: ORIGINAL ARTICLE doi: /circj.CJ

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1 726 MIYAZAKI A et al. Circ J 2017; 81: ORIGINAL ARTICLE doi: /circj.CJ Pediatric Cardiology and Adult Congenital Heart Disease Mid-Term Follow-up of School-Aged Children With Borderline Long QT Interval Aya Miyazaki, MD; Heima Sakaguchi, MD; Yu Matsumura, MD; Yosuke Hayama, MD; Kanae Noritake, MD; Jun Negishi, MD; Etsuko Tsuda, MD; Yoshihiro Miyamoto, MD; Takeshi Aiba, MD; Wataru Shimizu, MD; Kengo Kusano, MD; Isao Shiraishi, MD; Hideo Ohuchi, MD Background: There are no definitive diagnostic criteria or follow-up strategies for long QT syndrome (LQTS) in children with a borderline long QT interval (b-lqt). Methods and Results: We retrospectively evaluated the clinical course, genetic testing results, corrected QT interval (QTc), and LQTS score of 59 school-aged children (5 18 years old) with a b-lqt (400 QTc<500 ms). Syncope, but neither aborted cardiac arrest nor sudden cardiac death, occurred in 2 patients during the follow-up (6±3.4 years) with LQTS scores 4.5 points. The genetic testing results were positive in 92%, 57%, and 67% of patients with high, intermediate, and low probabilities of LQTS, respectively. The maximum and mean QTc during the follow-up significantly differed among the categories with a probability of LQTS, but not the minimum QTc. However, the QTc at rest and at the recovery point after exercise stress testing dramatically changed at the last followup. Consequently, the probability of LQTS changed in half of the patients. Conclusions: The LQTS score is a reasonable indicator for evaluating school-aged children with a b-lqt, and patients with a low LQTS score appear to be at low risk for cardiac events. However, the LQTS score can change during follow-up. Therefore, when there is doubt or concern for patients with a b-lqt, it is preferable to continue following them. Guidelines on follow-up strategies are desired for b-lqt. Key Words: Borderline long QT interval; Long QT syndrome; LQTS score; Mid-term follow-up; Schoolchildren Recently, the probability of a diagnosis of long QT syndrome (LQTS) was reported to be 1:3,298 in children aged 6 years and 1:988 in children aged 12 years based on the ECG school screening program in Japan. 1 Another study reported a high estimated probability of an aborted cardiac arrest (ACA) or sudden cardiac death (SCD) in school-aged children with LQTS, especially in boys with a corrected QT interval (QTc) 500 ms. 2 In the international LQTS registry study, the 5-year cumulative probability of ACA or SCD in children and adolescents with a QTc <500 ms with and without a history of syncope was reported to be 11% and 1%, respectively. 3 However, there are no recognized definitions of the upper and lower limits of a normal QT interval. The distribution of the QTc in the normal population and in individuals with LQTS overlaps between 400 and 500 ms. 4 The QTc values of the majority of the general population fall within Editorial p 640 a range of ms, during which LQTS remains possible. Despite comprehensive genetic testing of the currently known genes, approximately 15 20% of LQTS remains genetically elusive. 5 In a review of channelopathies published in 2013, 6 the considerable problems related to genetic testing were described as follows: For a patient who presents with a marginal phenotype, a positive genetic test, indicating a genetic variant, may not mean that they have the disease. Worse, there are patients who have a clear clinical phenotype and have a mutation, but the mutation is not linked to the disease. Instead, it may just be a normal variation, inconveniently residing as a bystander in the genome of a patient with that phenotype. Received September 29, 2016; revised manuscript received January 6, 2017; accepted January 12, 2017; released online February 18, 2017 Time for primary review: 18 days Department of Pediatric Cardiology (A.M., H.S., Y. Matsumura, Y.H., K.N., J.N., E.T., I.S., H.O.), Department of Cardiovascular Medicine, Division of Arrhythmias and Electrophysiology (T.A., W.S., K.K.), Department of Preventive Cardiology (Y. Miyamoto), Department of Preventive Medicine and Epidemiologic Informatics (Y. Miyamoto), National Cerebral and Cardiovascular Center, Suita; Department of Cardiovascular Medicine, Graduate School of Medicine, Nippon Medical School, Tokyo (W.S.), Japan Mailing address: Aya Miyazaki, MD, Department of Pediatric Cardiology, National Cerebral and Cardiovascular Center, Fujishirodai, Suita , Japan. ayamiya@ncvc.go.jp ISSN All rights are reserved to the Japanese Circulation Society. For permissions, please cj@j-circ.or.jp

2 Schoolchildren With b-lqt 727 Genetic testing in asymptomatic patients with a borderline LQT interval (b-lqt) seems to have less diagnostic significance than in symptomatic patients. Thus, there are no definitive diagnostic criteria for LQTS in patients with b-lqt, and it is unclear whether follow-up is necessary. However, among children with b-lqt, some have LQTS and are at risk of SCD, and their identification is critical. Therefore, in this study, we retrospectively evaluated the clinical characteristics of school-aged children with a b-lqt (400 QTc<500 ms) to determine the appropriate follow-up strategy. We assessed the following issues: clinical course during a mid-term follow-up; genetic testing results; and changes in the QTc and LQTS score during the follow-up. for a long QT interval. The authors assert that all procedures related to this work complied with the relevant national guidelines on human experimentation (Japan) and with the Declaration of Helsinki of 1975 (as revised in 2008), and were approved by the institutional ethics committees (Reference no. M28 019). Clinical Characteristics The following parameters were assessed: sex; age at the initial presentation; reason for the initial presentation; a family history of LQTS; genetic testing results; LQTS score at the initial presentation and at the end of the follow-up; ECG findings at the initial presentation, during Methods Patients The 122 consecutive pediatric patients were followed from February 1994 to April 2016 because they had prolonged QT interval. The patients who presented only once, and in whom the physician denied the possibility of LQTS and judged no necessity for follow-up, were excluded. The age distribution and reasons for the initial presentation of all patients are shown in Figure 1. We excluded 36 patients aged <5 years from this study, because an exercise tolerance test was unavailable in this age group and they presented at the hospital mainly because of a family history or symptoms. Moreover, 26 patients with a QTc 500 ms and who had already received medication were excluded to eliminate any factors unrelated to b-lqt. In total, b-lqt was retrospectively evaluated in 59 patients aged 5 years with a QTc 400 ms and <500 ms and without medication Figure 1. Age distribution and reasons for the initial presentation of all pediatric patients with borderline long QT interval. The 36 patients aged <5 years old were presented at the hospital mainly because of a family history (Fx) or symptoms. Table. Pediatric Patients Characteristics According to the Probability of Long QT Syndrome Based on the Long QT Syndrome Score Total High probability (LQTS score 3.5 points) Intermediate probability (LQTS score points) Low probability (LQTS score 1 point) P value n Age at the initial presentation (years) (12.4) (12.6) (12.2) (9.7) NS Reason for the initial presentation (#) School screening 48 (81%) 17 (85%) 24 (83%) 7 (70%) Family history of LQTS 6 (10%) 1 (5%) 3 (10%) 2 (20%) Symptoms 5 (8%) 2 (10%) 2 (7%) 1 (10%) Sex (M:F) 22:37 7:13 12:17 3:7 Family history of LQTS at the initial presentation (#) ECG findings at the initial presentation QTc (ms) 471± (487) (470) (451) < Resting heart rate (beats/min) 77±13 72±13 79±12 81±17 NS Notched T-wave in 3 leads (#) 0 Follow-up period (years) 6± ± ± ±4.0 NS Family history of LQTS at the last follow-up (#) Medication during follow-up (#) Follow-up period in the patients 6.1± ± ± ±4.5 NS without medication (years) (n=48) No. of resting ECGs during the follow-up in the patients without medication 16±9 16±8 17±9 15±11 NS LQTS, long QT syndrome; QTc, QT interval corrected using Bazett s formula. 12

3 728 MIYAZAKI A et al. Figure 2. Relationship between the long QT syndrome score at the initial presentation and a history of syncope/convulsions at the initial presentation (A) and cardiac events during the follow-up (B). The follow-up period was 6±3.4 years in 59 patients. No aborted cardiac arrests were observed, but new syncopal events occurred in 1 patient and convulsions in another. Convulsions in patients with a low probability of long QT syndrome were most likely clinically unrelated to cardiac arrhythmias. Hx, history. Figure 3. Results of genetic testing. (A) Relationship between the long QT syndrome (LQTS) score at the initial presentation and the results of genetic testing. (B) Ratio of a positive genetic test in patients with a high, intermediate, or low probability of LQTS. RyR2, ryanodine receptor gene. follow-up, and at the end of the follow-up; findings of the treadmill-based exercise stress test, cardiac events or convulsions before the initial presentation and during the follow-up; and medical treatments for long QT interval. The symptoms that prompted the initial presentation included chest pain, convulsions, and syncope. The LQTS score was calculated based on the criteria published by Schwartz and Crotti in A criterion referred to by the original LQTS score 8,9 was used for a low heart rate according to age (resting heart rate below the second percentile for age). The LQTS score at the initial presentation was retrieved from the data collected during the examination conducted at the initial presentation. At that time, family members who were not diagnosed with LQTS were not counted as LQTS family members. At the end of the follow-up, the QTc and LQTS score were only evaluated in the patients who were followed without any medication for QT prolongation. The latest ECG findings and results of the exercise stress test were used at the end of the follow-up. A history of syncope and family history of LQTS were updated during the follow-up. The categories of the probability of LQTS were the same as those used by Schwartz and Crotti: 7 low probability of LQTS represented 1 point, an intermediate probability points, and a high probability 3.5 points. Measurement of the QT Interval The QT interval was measured manually using the tangent method in lead V5 or V6 at rest and at the 4-min recovery point after the exercise stress test. 10,11 Subsequently, the QTc values were calculated using Bazett s formula. 12 In the case of sinus arrhythmia, the QT interval immediately following the shortest RR interval was used. 13 School Screening Program for Heart Diseases in Japan In Japan, there is a nationwide school-based ECG screen- Figure 4. Comparisons of the maximum (A), minimum (B), and mean (C) corrected QT (QTc) intervals during the followup between a high, intermediate, and low probability of LQTS in 48 patients without medication. The maximum and mean QTc intervals significantly differed between a high, intermediate, and low probability of LQTS; however, the minimum QTc did not differ. Open circle=non-genotyped patients; red, yellow, and orange diamonds=qtc in the patients with LQT1, LQT2, and LQT3, respectively.

4 Schoolchildren With b-lqt 729 Figure 5. Changes in the corrected QT (QTc) interval at rest (A) and at the 4-min recovery point after the exercise stress test (B) between the initial presentation and last follow-up in patients with a high, intermediate, and low probability of long QT syndrome. The QTc at rest was evaluated in 48 patients without medication during the follow-up (6.1±3.6 years). The QTc at the 4-min recovery point after the exercise stress test was evaluated in 32 patients who underwent the exercise stress test more than twice without medication (time between the initial and last exercise stress tests: 5.9±3.0 years). Open circle=non-genotyped patients; red, yellow, and orange diamonds=qtc in the patients with LQT1, LQT2, and LQT3, respectively. ing program for heart diseases for all 1st, 7th, and 10th graders (6 7, and years old). 1,10 This system consists of 3 steps. First, a standard 12-lead ECG is performed, and the children complete a questionnaire to determine any history of cardiac symptoms or a family history of SCD. Children with abnormal ECG findings and abnormal clinical and familial findings proceed to the second step of the examination. When the children are suspected of having cardiac problems, they are referred to a hospital for a more detailed examination. In this program, the detection range for a long QT interval is a QTc 450 ms at a heart rate <75 beats/min and 500 ms at a heart rate 75 beats/min. Also, the age- and sex-specific criteria using Fridericia s formula 14 are evaluated. The 10th graders (15 16 years old) generally visit the adult cardiology department, because the upper age limit for the atten- dance of pediatric departments in Japan is 15 years old. In this study, when patients visited hospital because of the school screening system, the ECGs obtained during the school screening were used as the ones for the initial presentation. Genetic Testing Although information on genetic testing was given to all patients, the genetic analysis was only performed in patients who consented. Patients with syncopal episodes, a family history of LQTS, or a QTc at rest 500 ms during the follow-up were strongly recommended to undergo genetic testing. The protocol for the genetic analysis was approved by the institutional ethics committee (Reference no. M ) and performed under its guidelines. Genomic

5 730 MIYAZAKI A et al. Figure 6. Changes in the long QT syndrome score (A) and probability of long QT syndrome (B,C) during the follow-up period in 48 patients without medication. The follow-up period was 6.1±3.6 years in 48 patients without medication. Open circle=nongenotyped patients. In (A), the red, yellow, and orange diamonds=the score in patients with LQT1, LQT2, and LQT3, respectively. Probability of long QT syndrome in 31 females (B) and in 17 males (C). DNA was isolated from whole blood using a DNA analyzer (QIAGEN GmbH, Hilden, Germany). 15 Genetic screening for mutations in the KCNQ1, KCNH2, and SCN5A (and, if necessary, KCNE1, KCNE2, and KCNJ2) genes was performed by direct sequencing (ABI 3730 DNA Analyzer; Life Technologies, Carlsbad, CA, USA). The cdna sequence numbering was based on the GenBank reference sequence. One patient in whom no mutations of KCNQ1, KCNH2, and SCN5A were detected underwent a whole-exon sequencing analysis to identify all the mutations in the known LQTS and candidate genes. 16 The captured DNA was sequenced using the HiSeq TM 2000 (Illumina, San Diego, CA, USA), as described previously. 17 As a result, a rare variant in the cardiac ryanodine receptor gene was detected. We enrolled this patient in the current study, because her ECG had a b-lqt and we followed her as a case of LQTS. We considered the mutations for which a link with LQTS has already been established in previous publications as disease-causing. In the absence of references, novel variants were included when they were deemed to be pathogenic or likely pathogenic by applying the recently published ACMG guidelines, 18 which are based on criteria using typical types of variant evidence, including the frequencies in the population data and computational (in silico) predictive programs. 19 Statistical Analysis Data are presented as the mean ± standard deviation or median (range) as appropriate, depending on their distribution (Shapiro-Wilk test), using JMP version 11 software (SAS Institute Inc., Cary, NC, USA). A two-tailed unpaired Student s t-test or Wilcoxon signed-rank sum test was used to compare the patient characteristics and the QTc among the categories of a probability of LQTS, as appropriate. A two-tailed paired t-test was used to com- pare the QTc and LQTS score during the follow-up. However, a statistical analysis was not performed to compare the cardiac events during the follow-up and a genetic analysis, because of the small population size. Results Clinical Characteristics at the Initial Presentation The age at the initial presentations of the 59 patients aged 5 years with a QTc 400 ms and <500 ms was 12.3 (range: ) years (male to female ratio 22:37).The reason for the initial presentation was a school screening in 48 patients (81%), family history of LQTS in 6 (10%), and symptoms in 5 (9%). The symptoms as the reason for the initial presentation included chest pain in 2 patients, syncope in 2, and convulsions in 1. The QTc on the resting ECG was 471±18 ms, and the LQTS score was 3 (range: 0 6.5) points. Based on the LQTS score, a high probability of LQTS (LQTS score 3.5 points) was evident in 20 patients (34%), an intermediate probability (1.5 3 points) in 29 (49%), and a low probability ( 1 point) in 10 (17%). The patients characteristics according to the probability of LQTS and the detailed characteristics in each patient are shown in Table and Table S1, respectively. There were 4 patients with a high or intermediate probability of LQTS who had a history of syncope; 2 with an intermediate or low probability of LQTS had a history of convulsions (Figure 2A); and 6 patients (10%) had a family history of LQTS. Clinical Course During the Follow-up During the follow-up period (6±3.4 years), no cases of ACA or SCD were observed, but syncope occurred in 2 patients with an LQTS score 4.5 points (Figure 2B). Convulsions occurred in 2 patients with an LQTS 1 point, but were most likely clinically unrelated to cardiac

6 Schoolchildren With b-lqt 731 arrhythmias. β-blockers were administrated to 11 patients (propranolol in 6 and bisoprolol in 5) after the initial presentation, for syncope in 4 patients, a prolonged QTc in 3, ventricular tachyarrhythmias during the epinephrine loading test or exercise stress test in 3, and convulsions of unknown etiology in one. One patient with a history of syncope and an LQTS score of 3 was not treated, because the syncopal episode was strongly suspected to have a neurologic origin. Genetic Characteristics Genetic tests were performed in 31 patients (54%) and the results are shown in Figure 3. Genetic mutations related to LQTS were detected in 11 (92%) of 12 patients with a high probability of LQTS, in 8 (57%) of 14 patients with an intermediate probability of LQTS, and in 4 (67%) of 6 patients with a low probability of LQTS. Changes in QTc Interval and LQTS Score in 48 Patients Without Medication During the Follow-up Among the 48 patients not receiving medication, a total of 777 ECGs were evaluated (16±9 per patient) during the follow-up period (6.1±3.6 years). The maximum and mean QTc during the follow-up significantly differed among the categories with a probability of LQTS, but not the minimum QTc (Figure 4). The QTc at rest changed dramatically by the last followup, albeit, there were no statistical differences (Figure 5A). Regarding the QTc at the 4-min recovery point after the exercise stress test, a dramatic change was also observed in 32 patients who underwent an exercise stress test without medication (time between the initial and last exercise stress tests: 5.9±3.0 years; Figure 5B). Consequently, the LQTS score also changed during the follow-up (Figure 6A). The categories of the probability of LQTS at the last follow-up remained the same as at the initial presentation in 23 of 48 patients without medication (48% of the total). In the other 25 (52%) patients, it changed between the initial presentation and last follow-up (Figure 6B). In females, it changed from a high to an intermediate probability in 2 of 10 patients (20%), and to a low probability in 1 (10%). Among the 16 patients with an intermediate probability of LQTS, it changed to a high probability in 5 (31%), and to a low probability in 8 (50%). In one of 5 patients (20%) with a low probability of LQTS it changed to a high probability and in one to an intermediate probability. In males, it changed from a high to an intermediate probability in 3 of 4 patients (75%). Among the 10 patients with an intermediate probability of LQTS, it changed to a high probability in 1 patient (10%) and to a low probability in 3 (30%). Discussion We examined the clinical characteristics of 59 school-aged children with a b-lqt (400 QTc<500 ms) who were suspected of having LQTS. No cases of ACA or SCD were observed, but syncope occurred in 2 patients with a high probability of LQTS during the mid-term follow-up. The genetic test was positive in 92%, 57%, and 67% of the patients with a high, intermediate, and low probability of LQTS, respectively. The maximum and mean QTc during the follow-up significantly differed among the categories with a probability of LQTS, but not the minimum QTc. The QTc at rest and at the 4-min recovery point after the exercise stress test dramatically changed between the initial presentation and the last follow-up. Consequently, the categories of the probability of LQTS changed by the last follow-up in half of the patients who were followed without meditation. Definition and Incidence of b-lqt The definition of a b-lqt varies greatly among the published studies. In a study by Viskin, the QTc values of a b-lqt were defined as ms for males and ms for females. 4 In the expert consensus statement of the Heart Rhythm Society, European Heart Rhythm Association, and Asia Pacific Heart Rhythm Society, it was stated that patients with a QTc of ms can only be diagnosed when they have a history of syncope. 20 Therefore, according to that statement, a b-lqt corresponds to a QTc of ms without unexpected syncope and <480 ms with or without syncope. In a study of an exercise-based algorithm for the prediction of LQTS published in 2011, a b-lqt was defined as a QTc of ms in males and ms in females. 11 In the nationwide school-based ECG screening program in Japan, a b-lqt is defined as a QTc 450 ms at a heart rate <75 beats/min, and 500 ms at a heart rate 75 beats/min. 1,10 It is difficult to determine the incidence of b-lqt because there is no agreed upon definition of the condition. However, in a school-based ECG screening program conducted in Kanazawa, Japan, the prevalence of a long QT interval ( 450 ms for boys and 460 ms for girls) in 7,961 schoolchildren was 1.1% in boys and 0.5% in girls. Among those 71 children with a long QT interval, 85% had an LQTS score 1 point. 21 In another study of the prevalence of a long QT interval as identified by the school-based screening program in Kagoshima, Japan, it was present in 0.2% of 32,982 1st graders (6 7 years old) and 0.3% of 34,572 7th graders. 1 Patients with a QTc 500 ms are diagnosed with LQTS in many studies, but the lower range of the QTc for b-lqt remains unclear. In this study, we defined patients with a QTc 400 ms and <500 ms as having b-lqt. We included patients with a QTc 400 ms and <450 ms who were suspected to have LQTS, because some of them had LQTS genes and some of them a prolonged QTc during the follow-up (Table S1). Methods of Evaluating b-lqt To date, there are 3 main methods of evaluating b-lqt: exercise stress test, epinephrine test, and LQTS score. 7,11,22 Among these, the LQTS score appears to be more useful and better suited for use in clinical situations, because it incorporates symptoms, family history, and the results from the exercise stress test. In this study, we evaluated school-aged children with b-lqt based on their LQTS score. During the follow-up period, syncope only occurred in patients with a high probability of LQTS based on the LQTS score. A positive genetic test occurred in 92% of patients with a high probability of LQTS, but in only 57% and 67% of those with an intermediate and low probability of LQTS, respectively. However, in half of the patients, the categorization of the probability of LQTS changed at final follow-up. The LQTS score is a reasonable method to detect LQTS among school-aged children with b-lqt, but we should recognize that it is not the perfect method.

7 732 MIYAZAKI A et al. Follow-up Strategy for b-lqt Our results indicated the arduousness of identifying LQTS from b-lqts by a 1-point evaluation. As it stands, when there is doubt or concern about patients with b-lqt, it is preferable to continue following them, because the cumulative rate of ACA or SCD is reported to be around 1 2% at 13 years old in children with a QTc <500 ms. 2 We propose that follow-up of patients with b-lqt exhibiting an intermediate or high probability of LQTS at the initial presentation and during the follow-up should be continued. Even in patients with a low probability of LQTS we would perform repeated ECGs, because some of them may show a prolonged QT interval during the follow-up. Study Limitations First, the sample size was small and the event rate was low. Second, this study retrospectively evaluated patients subject to sample selection bias, that is, the patients with a prolonged QT interval were judged by the physician as worthy of follow-up. We excluded any patients in whom the physician denied the possibility of LQTS and judged no necessity for follow-up. Third, the assessments were performed at various ages and during differing follow-up periods. Nevertheless, this study yielded important findings about school-aged children with b-lqt. Conclusions The LQTS score is a reasonable method of evaluating school-aged children with b-lqt, and patients with a low LQTS score appear to be at lower risk of cardiac events. However, the examination of the LQTS score is not perfect, and the LQTS score can change during the follow-up. It is difficult to identify LQTS from b-lqt by a 1-point observation. Therefore, when there is doubt or concern regarding patients with b-lqt, it is preferable to continue following them. Guidelines are needed for follow-up strategies in patients with b-lqt based on prospective studies involving larger numbers of patients. None declared. Conflicts of Interest Acknowledgments We express our gratitude to Mr. John Martin for his assistance in preparing the manuscript. Grants This work was partly supported by the Research Grant for Cardiovascular Diseases (H26-040) from the Ministry of Health, Labour and Welfare, Japan. References 1. Yoshinaga M, Kucho Y, Nishibatake M, Ogata H, Nomura Y. Probability of diagnosing long QT syndrome in children and adolescents according to the criteria of the HRS/EHRA/APHRS expert consensus statement. Eur Heart J 2016; 37: Goldenberg I, Moss AJ, Peterson DR, McNitt S, Zareba W, Andrews ML, et al. Risk factors for aborted cardiac arrest and sudden cardiac death in children with the congenital long-qt syndrome. Circulation 2008; 117: Liu JF, Jons C, Moss AJ, McNitt S, Peterson DR, Qi M, et al. Risk factors for recurrent syncope and subsequent fatal or near-fatal events in children and adolescents with long QT syndrome. J Am Coll Cardiol 2011; 57: Viskin S. 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HRS/EHRA/APHRS expert consensus statement on the diagnosis and management of patients with inherited primary arrhythmia syndromes: Document endorsed by HRS, EHRA, and APHRS in May 2013 and by ACCF, AHA, PACES, and AEPC in June Heart Rhythm 2013; 10: Hayashi K, Fujino N, Uchiyama K, Ino H, Sakata K, Konno T, et al. Long QT syndrome and associated gene mutation carriers in Japanese children: Results from ECG screening examinations. Clin Sci (Lond) 2009; 117: Shimizu W, Noda T, Takaki H, Nagaya N, Satomi K, Kurita T, et al. Diagnostic value of epinephrine test for genotyping LQT1, LQT2, and LQT3 forms of congenital long QT syndrome. Heart Rhythm 2004; 1: Supplementary Files Supplementary File 1 Table S1. Results of genetic testing, LQTS score, and QTc in 59 pediatric patients with a borderline long QT interval Please find supplementary file(s);