ART TRAINING - DAILY SCHEDULE Trainee: Godwin Akhabhoa, MBBS Training Dates: September15 - October 24, 2014

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1 WEEK DATE TIME FACULTY NAME PROGRAM 08:00 AM - 10:00 AM International center Orientation Drs. Agarwal, Sharma, Gupta, 10:00 AM - 12:30 PM Anagnostopoulou Discussion of Program Goals and Daily Schedule Mon 9/15/ :00 PM - 03:00 PM Drs. Anagnostopoulou Introduction to IVF/ICSI Week 1: Module 5D Tue 9/16/2014 Wed 9/17/2014 Thu 9/18/2014 Fri 9/19/2014 ART TRAINING - DAILY SCHEDULE Trainee: Godwin Akhabhoa, MBBS Training Dates: September15 - October 24, :00 PM - 05:00 PM Drs. Anagnostopoulou/Maldonado Lecture and demonstration: Embryo grading, embryo culture 09:00 AM - 10:00 AM Kevin Ryan Lecture on Optics of Micromanipulation 10:00 AM - 12:00 PM Drs. Anagnostopoulou/Maldonado Preparation of dishes, Introduction to micromanipulator and injection system 12:00PM - 12:30 PM ART PHOTO Portrait Picture Scheduled 01:30 PM - 04:30PM Drs. Anagnostopoulou/Maldonado Preparation of dishes, setting up of micromanipulator 09:00 AM - 10:00 AM Drs. Anagnostopoulou/Maldonado Lecture: Setting up and running an IVF laboratory 10:00 AM - 12:30 PM Drs. Anagnostopoulou/Maldonado Oocyte handling, sperm immobilization and aspiration 01:30 PM - 02:30PM SELF-STUDY SELF-STUDY 02:30 PM - 05:00 PM Drs. Anagnostopoulou/Maldonado Oocyte handling, sperm immobilization and aspiration 9:00 AM - 9:30 AM Drs. Anagnostopoulou Lecture: Essential instruments and disposable supplies for an IVF laboratory 09:30 AM - 12:30 PM Drs. Anagnostopoulou/Maldonado ICSI practice, oocyte denudation 02:00 PM - 04:30 PM Drs. Anagnostopoulou/Maldonado ICSI practice, oocyte denudation 09:00 AM - 9:30 AM Dr Anagnostopoulou Lecture: Extended culture and single embryo transfer 10:00 AM - 12:30 PM Dr. Anagnostopoulou/ Maldonado ICSI practice, embryo transfer 12:30-1:30 PM LUNCH BREAK LUNCH BREAK 1:30-2:00PM SELF-STUDY SELF-STUDY 02:00 PM - 05:00 PM Drs. Anagnostopoulou/Maldonado ICSI practice, embryo transfer 1

2 Week 2: Module 5D Mon 9/22/2014 Tue 9/23/2014 Wed 9/24/2014 Thu 9/25/2014 Fri 9/26/ :00 AM - 10:00 AM Dr Maldonado Lecture: Embryo evaluation, grading, and assisted hatching 10:00 AM - 12:30 PM Dr. Anagnostopoulou/ Maldonado ICSI practice, embryo transfer 03:00 PM - 05:00 PM Dr. Anagnostopoulou/ Maldonado ICSI practice, embryo transfer 09:00 AM - 11:30 AM Dr. Anagnostopoulou /Maldonado ICSI practice 11:30 AM - 12:15 PM Dr. Sajal Gupta Lecture: Role of assisted reproduction in treatment of female infertility 12:00 PM - 01:00 PM LUNCH BREAK LUNCH BREAK 01:00 PM - 01:30PM SELF-STUDY SELF-STUDY 01:30 PM - 05:00 PM Dr. Anagnostopoulou Maldonado ICSI practice 09:00 AM - 12:30 PM Dr. Anagnostopoulou ICSI practice 01:30 PM - 02:15PM Dr Sharma Lecture: Selection of Best Spermatozoa for ICSI 02:15 PM - 04:45 PM Dr. Anagnostopoulou ICSI practice 04:45 PM - 05:45 PM Dr. Sajal Gupta Powerpoint presentation "Controlled Ovarian Hyperstimulation" 02:00 PM - 04:30 PM Dr. Anagnostopoulou Maldonado ICSI practice 08:00 AM - 9:00 AM Dr Sajal Gupta Powerpoint presentation: Ovulation Induction protocols 12:30-1:30 PM LUNCH BREAK LUNCH BREAK 1:30-2:00pm SELF-STUDY SELF-STUDY 2:00 PM - 04:00PM Dr. Anagnostopoulou/ Maldonado ICSI practice 04:00 PM - 05:00 PM SELF-STUDY SELF-STUDY 2

3 Week 3: Module 1 Mon 9/29/2014 Tue 9/30/2014 Wed 10/01/2014 Thu 10/02/2014 Fri 10/03/ :00 AM - 10:00 AM Dr Rakesh Sharma Lecture: New WHO Standards for Semen Analysis 10:00 AM - 12:30 PM Dr. Sharma Sperm count, motility, round cell - practical demonstration + training 02:00 PM - 03:00 PM Dr. Sharma Leukocyte detection - lecture + practical demonstration 03:00 PM - 05:00 PM Dr. Sharma Vitality - lecture + practical demonstration + training 09:00 AM - 10:00 AM Dr. Sajal Gupta QC and Safety in Andrology laboratory 10:00 AM - 12:00 PM Dr.Sharma Sperm preparation for IVF/ICSI - lecture + practical demonstration 12:00 PM - 01:00 PM LUNCH BREAK LUNCH BREAK 01:00 PM - 02:30PM Dr. Sajal Gupta Sperm morphology - lecture + practical demonstration + training 02:30 PM - 05:00 PM Dr.Sharma Sperm count, motility, round cell - practical demonstration + training 09:00 AM - 10:00 AM Dr. Sajal Gupta Hypo-osmotic swelling test - lecture + practical demonstration 10:00 AM - 12:30 PM Dr. Sharma Sperm count, motility, round cell - practical demonstration 02:00 PM - 03:00 PM Dr Rakesh Sharma Lecture: Sperm Retrival techniques in azoospermia 03:00 PM - 05:00 PM Dr. Gupta Sperm morphology practice 09:00 AM - 12:30 PM Dr. Sajal Gupta Evaluation of azoospermic specimen(nfpics), CASA, antisperm antibody testing: Sperm MAR test - discussion + demonstration 02:00 PM - 04:30 PM Dr Sharma Semen analysis practice + scoring of vitality slides 04:30 PM - 05:00 PM Dr. Sharma Feedback session 08:00 AM - 10:00 AM Dr Sharma/Gupta Skill Testing and Grading 10:00 AM - 12:30 PM Debbie Garlak, MT Sperm MAR Antibody test 12:30-1:30 PM LUNCH BREAK LUNCH BREAK 1:30-2:00pm SELF-STUDY SELF-STUDY 2:00 PM - 04:00PM Dr Sharma Semen analysis practice + scoring of vitality slides 04:00 PM - 05:00 PM Dr Sharma Sperm wash practice 3

4 Week 4: Module 5D, 6 and 7 Mon 10/06/2014 Tue 10/07/2014 Wed 10/08/2014 Thu 10/09/2014 Fri 10/10/ :00 AM - 12:00 AM Dr. Anagnostopoulou/ Maldonado Slow cryopreservation 12:00 PM - 12:30 AM SELF-STUDY SELF-STUDY 02:00 PM - 04:00 PM Dr. Anagnostopoulou/ Maldonado Slow cryopreservation 04:00 PM - 05:00 PM SELF-STUDY SELF-STUDY 09:00 AM - 10:00 AM Dr Ashok Agarwal Introduction to Reproductive Laboratory TQM 10:00 AM - 11:00 AM Vincent Oziga, MD Self review of general and reproductive lab checklists 11:30 AM - 12:15 PM Dr. Sajal Gupta Andrology Laboratory QA/QC program 02:00 PM - 03:00 PM Dr Ashok Agarwal Designing QA/QC program for the IVF lab 09:00 AM - 10:00 AM Dr. Anagnostopoulou Trouble shooting In IVF laboratory 01:30 PM - 02:15PM Dr. Anagnostopoulou Fundamentals of Vitrification 02:00 PM - 04:45 PM Dr. Anagnostopoulou Practice & Introduction to Slow cryopreservation,vitrification of oocytes and embryos 04:45 PM - 05:45 PM Dr. Anagnostopoulou Hands on training for Vitrification Oocytes/Embryos 09:00 AM - 12:30 PM Dr. Anagnostopoulou Practice Vitrification session 02:00 PM - 04:30 PM Dr. Anagnostopoulou Slow freezing/vitrification practice 04:30 PM - 05:45 PM SELF-STUDY SELF-STUDY 09:00 AM - 12:30 PM Drs. Sharma/Gupta Quiz on lab accreditation, certification and licensure, scoring or results and discussion 01:30 PM - 2:30PM SELF STUDY SELF STUDY 02:30 PM - 04:30 PM Prepare for Presentation Prepare for Presentation 03:30 PM - 04:30 PM Vincent Oziga, MD/Godwin Akhaboa, Power Point Presentations: QA/QC Plan for Laboratory MBBS/Efiok Efiok Accreditation in your institution 04:30 PM - 05:00 PM Drs Agarwal/Gupta Wrap up of module 7 and 8 4

5 Week 5: Module 5D, 3 and 4 Mon 10/13/2014 Tue 10/14/2014 Wed 10/15/2014 Thu 10/16/2014 Fri 10/17/ :00 PM - 05:00 PM Dr. Anagnostopoulou/ Maldonado ICSI practice 02:00 PM - 05:45 PM Dr. Anagnostopoulou/ Maldonado ICSI practice 09:00 AM - 12:30 PM Dr Sharma Sperm selection and seperation techniques: Lecture/demonstration 01:30 PM - 04:45PM Dr Sharma Practice of sperm density gradient preparation 04:45 PM - 05:00 PM SELF-STUDY SELF-STUDY 09:00 AM - 12:30 PM Dr. Sharma Demonstartion and practice: Swim-up preparation 02:00 PM - 05:00 PM Dr Gupta Demonstartion and practice: Swim-up preparation 09:00 AM - 10:00 AM Dr Gupta Sperm Cryopreservation: Fundamentals of cryobiology 10:00 AM - 12:30 PM Dr Gupta Demonstraion and practice of sperm cryopreservation with andrology center protocols 01:30 PM - 2:30PM SELF STUDY SELF STUDY 02:30 PM - 04:30 PM Dr Sharma Practice: Sperm cryoprservation 03:30 PM - 04:30 PM Dr Gupta Lecture and protocol discussion: TESE/PESA 04:30 PM - 05:30 PM Drs Gupta/Sharma Q and A regarding sperm cryo 5

6 Week 6: Module 5D Mon 10/20/2014 Tue 10/21/2014 Wed 10/22/2014 Thu 10/23/2014 Fri 10/24/ :00 AM - 12:00 AM Drs. Anagnostopoulou/Maldonado Assisted hatching (chemical and laser) 12:00 PM - 12:30 PM SELF-STUDY SELF-STUDY 02:00 PM - 05:00 PM Drs. Anagnostopoulou/Maldonado Embryo biopsy - demonstration 09:00 AM - 12:00 PM Drs. Anagnostopoulou/Maldonado Embryo biopsy - practice 12:00 PM - 12:30 PM SELF-STUDY SELF-STUDY 01:30 PM - 05:00PM Drs. Anagnostopoulou/Maldonado PGD (Blastomere biopsy, laser and acid Tyrode's for zona drilling, polar body aspiration and fixation techniques and cell spreading) 09:00 AM - 12:30 PM Drs. Anagnostopoulou/Maldonado Slow cryoprservation of embryos 02:00 PM - 04:30 PM Drs. Anagnostopoulou/Maldonado Slow cryoprservation of embryos 09:00 AM - 12:30 PM Drs. Anagnostopoulou/Maldonado Vitrification practice 02:00 PM - 04:30 PM Drs. Anagnostopoulou/Maldonado Vitrification practice 09:00 AM - 10:30 AM Drs Agarwal/Gupta/Sharma/Agmnastopolou Feedback session 10:30 AM - 12:30 AM Drs. Anagnostopoulou/Maldonado Practice upon request 01:30 PM - 2:00PM SELF STUDY SELF STUDY 02:00 PM - 03:30 PM Drs. Anagnastopoulou/Maldonado Skill Testing and Grading 3:30 PM - 4:00 PM Godwin Akhaboa Powerpoint presentation: Embryo biopsy 4:00 PM - 5:00 PM Drs.Agarwal/Gupta/Sharma Anagnastopoulou/Maldonado Award of Certificate 6

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