Curriculum Vitae. Personal Record D. MANJULAKUMARI. Designation: Professor

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1 Curriculum Vitae Personal Record Name: D. MANJULAKUMARI Designation: Professor Address: Office: Dept. of Microbiology and Biotechnology Bangalore University Bangalore Date and place of Birth: Area of Specialization: Ethobiomolecules and Insect proteomics Academic Record Educational Qualification: M Sc., Ph.D. Professional Experience: Position Held Institution From To No. of Years Research Assistant Bangalore University Lecturer Bangalore University Lecturer (Senior Scale) Bangalore University Associate Professor Bangalore University Professor Bangalore University Date 7 th Research Experience: Research Guidance (Ph.D. awarded - 06) 1. Mr. Kumar Studies on physiological changes in the silkworm Bombyx mori L. following mating PhD awarded Ms. Shobha A Characterization of the accessory gland peptides in the insect Helicoverpa armigera PhD awarded Mr. Jagadeesh M.C Evaluation of the anticancer effects of embelin and curcumin PhD awarded Ms. Kumari Manju Isolation, characterization and identification of local isolates of Bacillus thuringensis Berliner active to Diptera, Coleoptera and Lepidoptera (Insecta) from Karnataka PhD awarded Ms. Medhini N Characterization of factors from Calendula officianilis influencing the physiology of a lepidopteran pest PhD awarded Mr. Divakara Y.G Isolation and Characterization of bioactive factors modifying physiology of oviposition in lepidopteran pest, Spodoptera litura PhD awarded Ms. Esther Shoba R Cloning and expression of chitinase gene from Helicoverpa armigera in yeast and study of insecticidal effect of purified chitinase on lepidopteran larvae Thesis submitted

2 8. Ms. Rama T Molecular Characterization of female reproductive behaviour modifying peptides in Helicoverpa armigera - Under progress 9. Ms. Tannavi Kiran Cloning and Expression of male factor influencing female reproductive physiology in Helicoverpa armigera H.- Under progress 10. Ms. Mamtha R Cloning and Expression of peptide inducing post mated behaviour in virgins of Spodoptera litura F., in a yeast system Under progress 11. Ms. Chaitra Protein profile of male accessory glands of Helicoverpa armigera H.: Purification and characterization of proteins transferred from male to female 12. Ms Saraswathi Studies on male origin proteins transferred to female in Leucinodes orbonalis Guenee, a pest on Solanum melongena L. Research project completed 1. Isolation and Characterization of Bioactive factors modifying physiology of oviposition in lepidopteran pest, Spodoptera litura (UGC) Lakhs Research project under progress: 1. Molecular characterization, cloning and expression of a male factor that affects female reproductive physiology and behaviour in lepidopteran pest, Helicoverpa armigera WOS-A, DST Mentor 24.5 Lakhs Research publications: 1. Esther Shoba R, Manmohan M, Santhosh S, Athmaram T N, Manjulakumari D. Heterologous expression of Helicoverpa armigera chitinase in Pichia pastoris. International Journal of Applied Research, Volume 2, Issue 3, , March Esther Shoba R, Athmaram T N, Manjulakumari D. Expression of a variant chitinase transcript of Helicoverpa armigera in E. coli. International Journal of Science and Research, Volume 5, Issue 3, , March Divakara Y.G. and Doddamane Manjulakumari. Artificial diet formulation and its efficacy evaluation on development and reproduction of Spodoptera litura F. A polyphagous pest. Indian journal of applied research, 5 (6) 55-57, June Divakara Y G, Mamtha R, Doddamane Manjulakumari. Characterization of Egg Maturation Substance from Spodoptera litura F. Males. International Journal of Science and Research. Volume 4 Issue 5, pages , May Manju kumari, D.Manjulakumari and ARV Kumar. Evaluation of local Bacillus thuringiensis from the soils of Western Ghats, Karnataka and their biocontrol potential against white grub, Holotrichia serrata (F.) (Coleoptera) and housefly, Musca domestica L. (Diptera) Asian Journal of Bioscience, 8(2), , Y.G. Divakara, N. Medhini and D. Manjulakumari. Bioactive factors from male accessory glands - a potent ecofriendly approach in the control of Spodoptera litura Fab. International Journal of Advanced Research. 1 (10), , Medhini N, Divakara Y.G., Prabha D and Manjulakumari D. Bioefficacy of Calendula officinalis Linn. (Asteraceae) extracts in the control of Spodoptera litura Fabricus (Noctuidae: Lepidoptera) under laboratory conditions. J. Biochem Tech. 3(5):S167-S169, Somayeh Dolatabadi and Manjulakumari D. Microbial Biosensors and Bioelectronics. Research Journal of Biotechnology, 7 (3), , Medhini N, Divakara Y.G. and Manjulakumari D. Effect of Calendula officinalis L. extracts on the nutrient components of different tissues of tobacco cutworm, Spodoptera litura. Journal of Biopesticides, 5: , N. Medhini, Y.G. Divakara and D. Manjulakumari. A profile of digestive enzymes activity in pot marigold, Calendula officinalis L. extracts treated tobacco cutworm, Spodoptera litura (F.). In: Insect Pest Management: A Current Scenario, (ed.) Dunston P. Ambrose, pp , 2011.

3 11. Divakara Y.G., Medhini N., and D. Manjulakumari. Mass rearing of tobacco cutworm, Spodoptera litura (fabricus.) under laboratory conditions on artificial diet and studies on their mating and egg laying behaviour. In: Insect Pest Management: A Current Scenario, (ed.): Dunston P. Ambrose, pp , Kumari manju., Manjulakumari D., Ravi Kumar B and Kumar A.R.V. Isolation of Bacillus thuringiensis (Berliner) colonies from the soil samples of Western Ghats, Karnataka and Evaluation of their biocontrol potential against White grub, Holotrichia serrata (F.) Coleoptera and House fly, Musca domestica L., Diptera. In: Insect Pest Management: A Current Scenario, (ed.): Dunston P. Ambrose, pp , Kumari manju., Manjulakumari D., Ravi Kumar B and Kumar A.R.V. Biocontrol potential of local strains of Bacillus thuringiensis (Berliner) against House fly, Musca domestica L. and Diamondback moth, Plutella xylostella L. In: Insect Pest Management: A Current Scenario, (ed.), Dunston P. Ambrose, pp , M.C. Jagadeesh, M. Sreepriya, Geetha Bali & D Manjulakumari. Protective Effects of Embelin and Curcumin Diethylnitrosamine / Phenobarbital Induced Experimental Hepatocarcinogenesis in Rats. International Journal of Life Sciences 5(1), 51-56, Shobha Anand., Geetha Bali and Manjulakumari Doddamane. Purification and partial characterization of a male derived peptide that enhances egg maturation and oviposition in female cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae). Entomon, 34(4), , Medhini N, Palaksha Prabhu K N, Divakara Y G, Kuntal Das and D Manjulakumari. Effect of Calendula officinalis L. extracts on consumption utilization indices of Spodoptera litura (Fab.) larvae. Karnataka Journal of Agricultural Sciences, 22, , Kumari Manju, Smitha George, A.R.V. Kumar and D Manjulakumari. Distribution of Bacillus thuringiensis Berliner strains in the soils of different habitats and their activity against white grubs. Karnataka Journal of Agricultural Sciences, 22, , M.C. Jagadeesh, M. Sreepriya, Geetha Bali and D. Manjulakumari. Biochemical studies on the effects of embelin and curcumin against N-nitrosodiethylamine(DENA)/ Phenobarbital (PB) induced hepatocarcinogenesis in rats. African Journal of Biotechnology, 8(18), pp , Manjulakumari Doddamane Shobha Anand and Geetha Bali. Relationship between egg maturation and oviposition and evidence of factors regulating these events in male ejaculatory duct duplex of Helicoverpa armigera. Entomon, 34(1), 1-10, Shobha,A., Manjulakumari D and Geetha Bali. Effect of mating in altering oviposition preference for substrata, photoperiod and diet in Helicoverpa armigera females. J.ent. Res. 33 (4): (2009). 21. Shobha,A., D. Manjulakumari and Geetha Bali. An inexpensive technique for Mass Rearing of Pod Borer, Helicoverpa armigera (Hubner). In: Recent Trends in Animal Behaviour. Ed., by Shakunthala Sridhara et.al., New India Publishing Agency, New Delhi. P , Manjulakumari D and Kumar. Male contribution of lipids to the female silkmoth, Bombyx mori L. during mating. Entomon, 32(4), , D. Manjulakumari and Geethabali. Innervation of hair sensilla present on the ovipositor of silkmoth, Bombyx mori.l Entomon 29(2): , Manjulakumari D. Ovipositional behavior of mulberry silkmoth Bombyx mori L. under different photic conditions Ann. Entomol., 19(2): 13-14, D. Manjulakumari and Geethabali. Mating: A mechanism that regulates oviposition behavior in the silk moth Bombyx mori L. In: Readings in behavior. Ed., by Ramamurthy and Geetha Bali, New Age International Ltd. P , G Varalakshmi, Geethabali and D. Manjulakumari. Neural mechanism of egg laying in silk moth Bombyx mori L. In: Readings in behavior. Ed., by Ramamurthy and Geetha Bali, New Age International Ltd. P , 1996.

4 27. D. Manjulakumari and Geethabali. Influence of mating on the temporal pattern of egg laying in the silk moth Bombyx mori L. Ann. Entomol, 11(2): D. Manjulakumari and Geethabali. Ovipositional response of Bombyx mori L. to different textures of the substratum. Entomon, 16(1), Geethabali and D. Manjulakumari. Ovipositon in silkworm and its control. Sericologia, 27(2), Impact of the research conducted either on the advance of knowledge or its practical significance Studies on the influences of disturbance, light and darkness, substratum on the oviposition behavior are of practical significance. The disturbance, light and rough substratum has a negative influence on the rate of egg laying. The use of plain substratum, maintenance of total darkness without disturbance in the grainages is cost effective as it saves a day labor (Manjulakumari D., 2001; D. Manjulakumari and Geethabali. 1991). For the first time, a reduced methylene blue technique was applied to study the innervation of hair sensilla on the ovipositor of silk moth by injecting the dye into the live animal. The technique revealed that each hair sensilla is innervated by a single bipolar neuron with the dendrite ending at the base of sensillum, a characteristic of mechanoreceptors. The axons of each nerve cell innervating these mechanoreceptors form a nerve bundle that enters the 9th abdominal ganglion (D. Manjulakumari and Geethabali, 2004) Egglaying is a segmental reflex in silkmoth and the last abdominal ganglion mediates this behavior. Brain is inhibitory for the function of egg laying in silkmoth. By isolating the last abdominal ganglion from the higher centers of nervous system the virgins are made to mimic the mated moths (G Varalakshmi, Geethabali and D. Manjulakumari. 1996). Mating is not a must for egg laying in silkmoths as virgins also lay eggs, but there is lot of difference in the egg laying behaviour of mated and virgin moths. Four parameters have been identified to quantify the egg laying behaviour and they are, the initiation of egg laying, rate of egg laying, total number of eggs laid and the life span (D.Manjulakumari and Geethabali, 1996 D. Manjulakumari and Geethabali, 1993, Geethabali and D. Manjulakumari, 1987). Very few laboratories in India have undertaken work on insect peptides/proteins in relation to reproduction in general and egg physiology in particular. The occurrence of an oviposition stimulating factor which also stimulates ovulation has been demonstrated in silkmoth Bombyx mori in our laboratory. Virgin moths injected with isolated male accessory gland factor could be made to exhibit mated behavior with respect to the pre-oviposition period, rate of egg laying and the number of eggs laid. (Bali and Manjulakumari, 1987; Manjulakumari and Bali, 1991, 1996). The chemical factor is identified as a protein with a molecular weight of 45KD. Male contribution of nutrients like proteins, lipids and glycogen to the female at the time of mating is evident from our studies on silk moth Bomyx mori. These nutrients provide energy for the post mating activities of female such as oviposition. The contribution of nutrients by male to female is of highly significance in lepidopteran insects which do not feed during their adult life (Manjulakumari D and Kumar, 2007). Two peptides in H.armigera, one that stimulated egg maturation and oviposition and the other that inhibited behaviors like calling and mating in treated virgin females have been isolated in our laboratory. (Shobha et al., 2008).

5 Identification of these peptides which modify the female behaviour opens up the possibility of employing them as biopesticides by introducing these peptides to virgins and thus inhibiting pre-mating and inducing Post- mating activities in virgins. This could be achieved through recombinant DNA technology by expressing these peptides possibly in baculovirus. The gene encoding chitinase protein in Helicoverpa armigera has been isolated and the protein is successfully expressed in bacteria (E. coli) and Yeast (Pichia pastoris) system. We have demonstrated the antifeedancy, oviposition deterrence and ovicidal activity of Calendula officinalis L. extracts against Spodoptera litura a polyphagus pest on Agicultural crops. Two compounds Alpha-antiarin and Oryzanol-A responsible for oviposition deterrent and ovicidal activity against the pest have been isolated which have potential to be used as biopesticides.