Success of intracytoplasmic sperm injection in couples with male and/or female chromosome aberrations
|
|
- Damian Lee
- 6 years ago
- Views:
Transcription
1 Human Reproduction vol.12 no.12 pp , 1997 Success of intracytoplasmic sperm injection in couples with male and/or female chromosome aberrations M.Montag 1,5, K.van der Ven 1, S.Ved 1, A.Schmutzler 1, G.Prietl 1, D.Krebs 1, B.Peschka 2, G.Schwanitz 2, P.Albers 3, G.Haidl 4 and H.van der Ven 1 1 Department of Endocrinology and Reproductive Medicine, 2 Department of Human Genetics, 3 Department of Urology, 4 Department of Dermatology, University of Bonn, Bonn, Germany 5 To whom correspondence should be addressed This paper reports on results of intracytoplasmic sperm injection (ICSI) in patients in whom constitutional or secondary chromosome aberrations were detected in the male and/or female partner. Out of 434 couples treated by ICSI (590 cycles), 16 couples (3.7%) were affected by constitutional chromosome aberrations and 96 (22.1%) by secondary chromosome aberrations. Constitutional chromosome aberrations were found in eight male and eight female patients. Couples with the aberration in the male showed significantly lower fertilization, implantation and pregnancy rates (P < 0.05). The occurrence of female constitutional chromosome aberrations led to lower fertilization rates but implantation and pregnancy rates were similar to a control group; however, a higher abortion rate was noted. In the group with secondary chromosome aberrations, 22 males and 59 females carried an abnormality and in 15 couples, both partners. Compared to the remaining (unaffected) 322 couples, fertilization and embryo transfer rates were reduced but implantation rates and pregnancy rates were not different. In all couples where an abortion occurred, mainly parental autosomal aberrations were involved (six out of eight). Our retrospective analysis shows that an unexpectedly high number of infertile couples in an ICSI programme are affected by chromosome aberrations, which in turn may explain the reduced fertilization rates observed in this subgroup of patients. Key words: constitutional chromosome aberrations/genetic risk/ intracytoplasmic sperm injection/secondary chromosome aberrations Introduction Since its introduction in 1992 (Palermo et al., 1992), intracytoplasmic sperm injection (ICSI) has turned out to be one of the most successful assisted reproductive techniques. This has raised the question of the genetic consequences of ICSI, not only with regard to the karyotype of the children born, but also to a possible predisposition of the infertile couple (Silber et al., 1995; Chandley and Hargreave, 1996; Fishel et al., 1996; Martin, 1996; Persson et al., 1996). Several studies have shown a higher incidence of chromosome abnormalities in infertile males (Chandley et al., 1975), with an overall incidence of 7.1% constitutional aberrations (Retief et al., 1984). Most frequently, sex chromosome aneuploidies have been reported (De Braekeleer and Dao, 1991). As a consequence, in cases of male infertility it is now common practice in most in-vitro fertilization (IVF) centres to perform a cytogenetic screening of the male partner prior to ICSI treatment. It has previously been recognized that the outcome of ICSI might be influenced by the occurrence of chromosome abnormalities in the female partner (Meschede et al., 1995). Recently, we reported an increased frequency of constitutional chromosome aberrations in male and female partners of couples examined prior to ICSI (Peschka et al., 1996; van der Ven et al., 1998). In 19 out of 305 couples (6.23%), at least one partner was affected by a constitutional chromosome aberration and in one case both were affected. Whereas the rate of abnormality in the male (3.27%) was within the expected range for men with impaired semen parameters, the rate for the females (3.27%) was unexpectedly high. The data indicate that the potential contribution of maternal chromosome aberrations in cases of poor reproductive outcome cannot be neglected. Further research is needed concerning the role of maternal aneuploidy in failed human embryo implantation. Oocyte aneuploidy is found commonly among older women, and similar poor reproductive outcomes apply to women affected by a structural chromosome abnormality (Plachot et al., 1988; Angell et al., 1993; Munné et al., 1995). Routine cytogenetic analysis of both the male and the female partner is part of our infertility programme. In this paper, we have evaluated the relevance of the occurrence of constitutional as well as secondary chromosome aberrations (SCA) in the male and female for the success rate of ICSI. Materials and methods Andrological, gynaecological and cytogenetic examination Between June 1994 and October 1996, a total of 434 couples underwent 590 ICSI treatment cycles at the University of Bonn. The selection for ICSI treatment was based on the diagnosis of male factor infertility with reduced sperm quality. In a collaborative approach, all patients underwent an extensive andrological, gynaecological and cytogenetic examination prior to ICSI. For all patients a chromosome analysis was performed on peripheral lymphocytes. Up to 50 metaphases ( bands per genome) European Society for Human Reproduction and Embryology 2635
2 M.Montag et al. were analysed per patient. The classification of secondary chromosome aberration (SCA) comprises the occurrence of one or more single cells showing either a structural or numerical chromosome abnormality of the same karyotype. However, in some cases we found several abnormal cells which exhibited a combination of different chromosome aberrations, mainly combined autosomal and sex chromosomal aberrations. The occurrence of two abnormal single cells within one preparation exhibiting a complementary aberration (e.g. 45, XO and 47, XXX) was considered to be an artefact. In complex structural rearrangements, additional molecular cytogenetic analysis by fluorescence in-situ hybridization (FISH) was performed. Patients identified as carriers for chromosomal abnormalities underwent genetic counselling to explain the importance of the findings and the implications for their offspring. Furthermore, in all individuals with constitutional aberrations, additional investigations of first and second degree relatives were recommended. Ovarian stimulation Follicular stimulation was carried out by the combination of the gonadotrophin releasing hormone agonist (GnRHa) triptorelin acetate (Decapeptyl, Ferring, Germany), human menopausal gonadotrophin (HMG; Humegon, Organon) and/or follicular stimulating hormone (FSH; Fertinorm, Serono) and human chorionic gonadotrophin (HCG). Triptorelin acetate (0.1 mg/day) was administered from day 22 of the previous cycle. Twelve to 15 days later, HMG/FSH (225 IU) was administered daily. Ovarian response was monitored by transvaginal ultrasound and HMG/FSH was adjusted according to the patient s individual response based on follicular size and oestradiol levels. HCG ( IU) was administered when the leading follicles were 18mm in diameter. Intracytoplasmic sperm injection All media used for oocyte retrieval, denuding, ICSI treatment and subsequent culture were of pharmaceutical grade and free of phenol red (IVF-50; Gamete-100, ICSI-1; Scandinavian IVF Science, Göteborg, Sweden). For injection, sperm cells were prepared by a modified mini-swim-up technique. The liquefied ejaculate was washed once with Gamete-100. The sperm pellet was dissolved in 1 ml of medium, recentrifuged in a microfuge (Biofuge 13, Heraeus, Osterode, Germany) and the final pellet was resuspended in µl of medium and stored in a CO 2 incubator. A few microlitres of the sperm suspension was placed into a central polyvinylpyrrolidone (PVP) droplet (ICSI-1) in the injection dish. The technique used for injection followed essentially the protocol published by Palermo et al. (1995). ICSI was carried out on the heated stage of an inverted microscope (DMIRB; Leica, Bensheim, Germany) equipped with microinjection devices for holding the oocyte and sperm injection (Narishige, Tokyo, Japan). Following injection, oocytes were cultured in IVF-50 up to the time of transfer. Transvaginal intrauterine embryo transfer of a maximum of three embryos took place 2 days following oocyte retrieval. Luteal phase support was performed with progesterone vaginal suppositories (2 200 mg per day) starting on the day following ovulation induction with HCG. Pregnancy was defined as the occurrence of a positive β-hcg ( 10) value at day 12 after transfer and a second, higher value 2 days later. Only pregnancies reaching HCG values 100 were considered for the evaluation. Implantation was determined after ultrasonic detection of a gestational sac and viability was demonstrated by a positive heart beat. Statistics Couples with constitutional chromosome aberrations were compared with the total number of patients without chromosome aberrations (unaffected couples) and a matched control group of patients identified in a computer database and matched by sperm analysis, women s age, number of treatment cycles (16 patients; 30 cycles) and number of oocytes injected. Data were analysed by χ 2 test and P values of 0.05 were considered significant. Results Type and frequency of chromosome aberrations A total of 434 couples undergoing 590 ICSI cycles from June 1995 to October 1996 were evaluated cytogenetically. In all, 322 couples (74.2%) showed no chromosome aberrations and were regarded as unaffected (Table I). In 112 couples (25.8%), a chromosome abnormality was detected. Out of these, 16 couples (3.7%) were affected by a constitutional aberration, present in either the male (eight couples) or the female partner (eight couples). All patients with constitutional aberrations are listed in detail in Table II. The remaining 96 couples (22.1%) showed SCA. The type and frequency of SCA are shown in Table III. The frequency of SCA ranged from 2 to 20% of all cells examined and the mean value of abnormal cells per affected patient was 3.1% for autosomal, 3.6% for sex chromosomal and 6.6% for combined autosomal and sex chromosomal aberrations. In cases of sex chromosomal SCA, we found mainly numerical aberrations (76 versus 24% structural aberrations), whereas for autosomal SCA, structural abnormalities were dominant (76%). Patients with SCA were further allocated to subgroups. Accordingly, groups were defined with couples where the male partner (22 couples), the female partner (59 couples) or both partners (15 couples) were affected (see Table I). ICSI results in patients with constitutional chromosome aberrations We first addressed the question of whether constitutional chromosome aberrations had a deleterious effect on ICSI results (Table IV). A total of 30 treatment cycles was performed in 16 couples, with 20 male and 10 female affected cycles. Fertilization rates were significantly higher in the matched control group and in the unaffected group than in the male and female affected group (P 0.05). For all groups, the transfer rates were not significantly different. The implantation rates per embryo transferred were comparable in the female affected group, the matched control group and the unaffected group, whereas the male affected group exhibited a significantly lower implantation rate (P 0.05). Pregnancy rates for the matched control group, the unaffected group and the female affected couples were comparable (28.6, 26.1 and 33.3%), Table I. Incidence and type of chromosome aberration of males and females in 434 male infertility couples Unaffected Constitutional Single cell aberrations aberrations Couples (%) 322/434 (74.2) 16/434 (3.7) 96/434 (22.1) Males (%) 322/434 8/434 (1.9) 37/434 a (8.5) Females (%) 322/434 8/434 (1.9) 74/434 a (17.1) a In 15 couples both partners were affected. 2636
3 ICSI in couples with chromosome aberrations Table II. Type of constitutional chromosome aberration in 16 male and female patients Patient Age Karyotype No. of Pregnancy cycles Sex chromosomal C95/ ,XXX 1 0 (numerical) C96/ ,XXY 2 0 Autosomal C95/ ,XY,t(1;2)(p34.1;p21) 2 0 Reciprocal C95/ ,XY,t(4;5)(q21;q11.2) 1 0 translocations C95/ ,XY,t(1;21)(1;9;21) 3 0 C96/ ,XY,t(3;12)(p24;p12) 3 0 C96/ ,XY,t(1;5)(p32;q31) 3 1 C95/ ,XX,t(5;19)(p10;q10) 3 1 a C96/ ,XX,t(3;18)(q24;p11.3) 1 1 a Robertsonian C95/ ,XY,der(13;14)(q10;q10) 2 0 translocations C95/ ,XX,der(13;14)(q10;q10) 2 0 C96/ ,XX,der(14;15)(q10;q10) 1 1 Inversions C95/ ,XY,inv(5)(p14.2;q22) 1 0 Other C95/ ,XY,der(9) 3 0 structural C95/ ,XX,der(9)add(9)(p12) 1 0 aberrations C95/ ,XX,der(9)add(9)(p12) 1 0 a Aborted in the first trimester. Table III. Type and frequency of abnormal single cells detected in lymphocytes in couples with SCA Chromosomes No. of patients Specification and Mean percentage of Range of abnormal affected a frequency of SCA abnormal single cells single cells (%) (%) per patient (%) Autosomes 23 females Structural: males Numerical: 24 Sex chromosomes 24 females Structural: males Numerical: 76 Auto- and 12 females Structural: 23 sex chromosomes 5 males Numerical: (combined) Combined: 39 (structural and numerical) a In another 15 couples both partners were affected. SCA secondary chromosome aberrations. Table IV. Results of intracytoplasmic sperm injection in patients with constitutional chromosome aberration compared to patients with no chromosome aberration (matched control group and unaffected couples) Constitutional chromosome aberration Control group Total Male partner Female partner Matched couples Unaffected couples affected affected No. of couples Mean age of the female No. of cycles Fertilization rate 93/252 a 61/156 a 32/96 a 125/251 a 2298/4016 a (36.9) (39.1) (33.3) (49.8) (57.2) No. of transfers No. embryos transferred/cycle 76/28 50/19 26/9 74/28 113/ Implantation rate per 4/76 c 1/50 b 3/26 c 11/74 b,c 118/387 embryo transferred (5.3) (2) (7.7) (14.9) (10.6) Clinical pregnancy rate 4/28 1/19 d 3/9 e 8/28 d,e 101/387 per transfer (14.3) (5.3) (33.3) (28.6) (26.1) Abortions Ongoing pregnancy rate (%) a P 0.01 for Fertilization rate. b P 0.05; c n.s. for Implantation rate per embryo transferred. d P 0.05; e n.s. for Clinical pregnancy rate per transfer. Values in parentheses are percentages. 2637
4 M.Montag et al. Table V. Results of intracytoplasmic sperm injection in patients with secondary chromosome aberration compared to the remaining unaffected couples Secondary chromosome aberration Total affected Female affected Male affected Male and female Control unaffected affected couples No. of couples Mean age of the female No. of cycles Fertilization rate 627/ / / / /4016 (36.5) b (36.2) ab (35.6) ab (38.6) ab (57.2) b No. of transfers (91.6) d (96.9) cd (81.3) cde (90.9) de (98.2) d No. embryos transferred 391/ /93 95/39 54/ /387 per transfer cycle Implantation rate 48/391 33/242 9/95 6/54 118/387 per embryo transferred (12.3) (13.6) (9.5) (11.1) (10.6) Clinical pregnancy rate 37/152 26/939 6/39 5/20 101/387 per transfer (24.3) (27.9) (15.4) (25) (26.1) Abortions 8/37 5/26 1/6 2/5 19/101 (21.6) (19.2) (16.7) (40) (18.8) Ongoing pregnancy rate (%) a n.s.; b P 0.05 for fertilization rate. c,d P 0.05; e n.s. for no. of transfers. Values in parentheses are percentages. whereas the male affected group showed a significantly lower pregnancy rate (5.3%) (P 0.05). The correlation of the type of constitutional aberration and the ICSI results is also shown in Table II. We have observed one pregnancy in the male affected group and three in the female affected group. For the latter, one pregnancy occurred in a case of Robertsonian translocation and two in females with reciprocal translocations; these fetuses were both aborted. ICSI results in patients with secondary chromosome aberrations We then examined the effect of SCA on ICSI. The 96 couples with this type of aberration underwent 166 ICSI cycles (Table V). In the majority of ICSI treatment cycles, chromosomal affection originated from the female partner (96 cycles versus 48 cycles). Fertilization rates were between 35.6 and 38.6% and not significantly different between couples where the male, the female or both partners were affected. However, when compared to the remaining, unaffected patients, all subgroups showed significantly lower fertilization rates (P 0.05). The same was noted for the transfer rates, which were even significantly different between the subgroups of male and female affected couples. Implantation rates and pregnancy rates were not significantly different within affected groups or when compared to the group of unaffected couples. A high abortion rate was noted when both partners were affected (40%). Overall, most abortions (six out of eight) occurred in couples where autosomal aberrations were found (data not shown). In the unaffected couples, the abortion rate was 18.8%. Discussion This paper reports the results obtained by ICSI in couples where both male and female partners received a routine 2638 cytogenetic examination prior to ICSI treatment. Out of 590 ICSI treatment cycles which we performed, 196 (33.2%) involved patients with either constitutional or secondary chromosome abnormalities. Abnormalities were found in male and female partners of couples treated for male infertility. Reduced fertilization rates in affected patients We observed reduced fertilization rates in all couples with chromosome aberrations. It is tempting to speculate that this might be an indicator for a developmental mechanism leading to selection at the earliest stage of gamete interaction following ICSI. Several authors have reported on the occurrence of chromosomal disorders in seemingly unfertilized or failedfertilized oocytes (Pellestor, 1991; Asch et al., 1995; Wall et al., 1996). These anomalies correlate with maternal age (Angell et al., 1993; Munné et al., 1995) or ovarian stimulation therapy (Wramsby et al., 1987), and were shown to cause reduced fertilization rates (Plachot and Crozet, 1992). It is uncertain whether this applies to our female affected groups, as the mean maternal age for women with SCA was on average years and for women with constitutional aberrations years. The mean age of the female in the unaffected control groups ( years) was within the same age range and the protocols for ovarian stimulation were identical for all patients. Therefore, the reduced fertilization rate may possibly be caused by the presence of chromosome aberrations. This hypothesis holds true for male constitutional aberrations, which showed a significantly lower fertilization rate when compared to the female affected groups. It is well known and widely accepted that fertilization failures can also be due to a reduced fertilization potential of the spermatozoa. This can be caused either directly by constitutional chromosome aberrations (Estop et al., 1995) or by failures during spermatogenesis, for example, defects of the sperm centrosome (Van Blerkom, 1996), sperm nuclear chromatin packaging (Bianchi et al.,
5 ICSI in couples with chromosome aberrations 1996) or genomic imprinting abnormalities (Tesarik and Mendoza, 1996). Both autosomal and sex chromosomal genes appear important in the evolving genetic understanding of spermatogenesis (Tiepolo and Zuffardi, 1976; Saxena et al., 1996). The role of secondary chromosome aberrations To date, the significance of SCA has not been assessed in detail, although Toncheva et al. (1994) reported that the presence of sex chromosomal aberrations at low frequency might be an underestimated cause for failure in assisted reproduction. However, it needs to be clarified whether the incidence of SCA (sex chromosomal and autosomal) is increased in ICSI couples. Our results, and those of others, indicate that the presence of SCA in lymphocyte cultures is highly unlikely to be an artificial result attributed to cellular stimulation and culture or preparation of chromosomes for karyotyping (Iskra, 1985). We cannot precisely state at present whether SCA is an indicator of low-frequency mosaicism or whether it simply reflects a greater chromosomal instability within affected individuals. To what extent SCA might cause a reduced fertilization rate is still unclear. It is possible that SCA also occur in cells in the germ line and therefore might have an impact on germ cell development and subsequent fertilization ability. Therefore, it would be interesting to analyse the chromosomal constitution of germ cells derived from male and female patients with documented SCA and to compare the results to those reported in the literature for normal individuals (Brandriff et al., 1990; Martin et al., 1992; Lee et al., 1996). New techniques for the analysis of aneuploidy in spermatozoa, for example, multicolour FISH, are promising and allow the discrimination of diploid, disomic or haploid spermatozoa (Rademaker et al., 1997). Pregnancy rates in affected couples In our study, couples with male constitutional aberrations have an especially poor prognosis to achieve an ongoing pregnancy by ICSI. At first sight, our results are in contradiction to those recently reported by Testart et al. (1996). These authors did not find a difference between pregnancy rates in couples with male and/or female structural aberrations and a control group. However, both study groups differ in their composition, especially in regard to the type of male chromosome aberration. In our study group, we report five cases with male reciprocal translocations (versus two cases in Testart s group) resulting in one pregnancy following 12 ICSI attempts. With regard to Robertsonian translocations, Testart and colleagues identified only affected males (nine ICSI attempts, three clinical pregnancies), whereas we found only one male and two females affected, and for the females one pregnancy occurred in three ICSI attempts. Experimental data are available which suggest a selection against unbalanced spermatozoa during spermatogenesis in males carrying Robertsonian translocations (Pellestor et al., 1987). This may explain the high pregnancy rates reported by Testart et al. for this type of translocation. However, this indicates the need for additional studies to establish the relationship between the type of constitutional chromosome aberration and the results after ICSI. Abortion rates in affected couples We noted high abortion rates when female patients were affected by constitutional chromosome aberrations, mainly reciprocal translocations, or when both partners were affected by SCA. Data are insufficient to draw conclusions regarding the risk of spontaneous abortion in individual subgroups. However, most abortions were found in couples with autosomal (structural) aberrations and these types of aberrations are probably less viable than sex chromosomal ones. Unfortunately, it was not possible to perform cytogenetic analysis of aborted tissue. So far, we have been unable to perform a cytogenetic analysis of the children born from SCA couples in order to evaluate whether the same type of SCA detected in the male or female could be transmitted to the offspring. Based on the results of this study, it may be useful to perform pre-implantation genetic diagnosis in patients with constitutional chromosome aberrations which showed low implantation rates and high abortion rates. Our data clearly support the argument for strict cytogenetic screening of male and female ICSI patients prior to treatment. This may allow better counselling, especially of patients with a chromosome aberration who are at high risk of miscarriage. Acknowledgements The authors would like to thank Ulla Dieckmann and Dr Ricarda Lange for their help in the data acquisition process and the staff of the IVF Unit and the Institute of Human Genetics for their assistance. References Angell, R.R., Xian, J. and Keith, J. (1993) Chromosome anomalies in human oocytes in relation to age. Hum. Reprod., 8, Asch, R., Simerly, C., Ord, T. et al. (1995) The stages at which human fertilization arrests: microtubule and chromosome configuration in inseminated oocytes which failed to complete fertilization and development in humans. Mol. Hum. Reprod., 1 in Hum. Reprod., 10, Bianchi, P.G., Manicardi, G.C., Urner, F. et al. (1996) Chromatin packaging and morphology in ejaculated human spermatozoa: evidence of hidden anomalies in normal spermatozoa. Mol. Hum. Reprod., 2, Brandriff, B.F., Gordon, L.A. and Carrano, A.V. (1990) Cytogenetics of human sperm: structural aberrations and DNA replication. In Mendelsohn, M.L. and Albertini, R.J. (eds), Mutation and Environment. Part B: Metabolism, Testing Methods and Chromosomes. Wiley-Liss, New York, NY, pp Chandley, A.C. and Hargreave, T.B. (1996) Genetic anomaly and ICSI. Hum. Reprod., 11, Chandley, A.C., Edmond, P., Christie, S. et al. (1975) Cytogenetics and infertility in man. Ann. Hum. Genet., 39, De Braekeleer, M. and Dao, T.N. (1991) Cytogenetic studies in male infertility: a review. Hum. Reprod., 6, Estop, A.M., Marquez, C., Munné, S. et al. (1995) An analysis of human sperm chromosome breakpoints. Am. J. Hum. Genet., 56, Fishel, S., Aslam, I. and Tesarik, J. (1996) Spermatid conception: a stage too early, or a time too soon? Hum. Reprod., 11, Iskra P. (1985) Somatic chromosome rearrangements detected in human lymphocytes. Clin. Genet., 28, 438A. Lee, J.D., Kamiguchi, Y. and Yanagimachi, R. (1996) Analysis of chromosome constitution of human spermatozoa with normal and aberrant head morphologies after injection into mouse oocytes. Hum. Reprod., 11, Martin R.H. (1996) The risk of chromosomal abnormalities following ICSI. Hum. Reprod., 11,
6 M.Montag et al. Martin, R.H., Rademaker, A.W., Ko, E. et al. (1992) A comparison of the frequency and type of chromosomal abnormalities in human sperm after different sperm capacitation conditions. Biol. Reprod., 47, Meschede, D., De Geyter, C., Nieschlag, E. et al. (1995) Genetic risk in micromanipulative assisted reproduction. Hum. Reprod., 10, Munné S., Dailey, T., Sultan, K.M. et al. (1995) The use of first polar bodies for preimplantation diagnosis of aneuploidy. Mol. Hum. Reprod., 1 in Hum. Reprod., 10, Palermo, G., Joris, H., Devroey, P. et al. (1992) Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet, 340, Palermo, G., Cohen, J., Alikani, M. et al. (1995) Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility. Fertil. Steril., 63, Pellestor, F. (1991) Differential distribution of aneuploidy in human gametes according to their sex. Hum. Reprod., 6, Pellestor, F., Sèle, B. and Jalbert, H. (1987) Chromosome analysis of spermatozoa from a male heterozygous for a 13;14 Robertsonian translocation. Hum. Genet., 76, Persson J.W., Peters G.B. and Saunders D.M. (1996) Is ICSI associated with risks of genetic disease? Implications for counselling, practice and research. Hum. Reprod., 11, Peschka, B., Schwanitz, G., van der Ven, K. et al. (1996) Type and frequency of constitutional chromosome aberrations in couples undergoing ICSI. Hum. Reprod., 11, Plachot, M. and Crozet, N. (1992) Fertilization abnormalities in human invitro fertilization. Hum. Reprod., 7, Plachot, M., de Grouchy, J., Junca, A.M. et al. (1988) Chromosome analysis of human oocytes and embryos: does delayed fertilization increase chromosome imbalance. Hum. Reprod., 3, Rademaker, A., Spriggs, E., Ko, E. et al. (1997) Reliability of estimates of diploid human spermatozoa using multicolour fluorescence in-situ hybridization. Hum. Reprod., 12, Retief, A.E., van Zyl, J.A., Menkveld, M.F. et al. (1984) Chromosome studies in 496 infertile males with a sperm count below 10 million/ml. Hum. Genet., 66, Saxena, R., Brown, L.G., Hawkins, T. et al. (1996) The DAZ gene cluster on the human Y chromosome arose from an autosomal gene that was transposed, repeatedly amplified and pruned. Nature. Genet., 14, Silber, S.J., Nagy, Z., Liu, J. et al. (1995) The use of epididymal and testicular sperm injection: the genetic implications for male infertility. Hum. Reprod., 10, Tesarik, J. and Mendoza, C. (1996) Genomic imprinting abnormalities: a new potential risk of assisted reproduction. Mol. Hum. Reprod., 2, Testart, J., Gautier, E., Brami, C. et al. (1996) Intracytoplasmic sperm injection in infertile patients with structural chromosome abnormalities. Hum. Reprod., 11, Tiepolo, L. and Zuffardi, O. (1976) Localization of factors controlling spermatogenesis in the nonfluorescent portion of the Y chromosome long arm. Hum. Genet., 34, Toncheva, D., Ilieva, P. and Mavrudieva, M. (1994) Detection of low level sex-chromosomal mosaicism. Genet. Couns., 5, Van Blerkom, J. (1996) Sperm centrosome dysfunction: a possible new class of male factor infertility in the human. Mol. Hum. Reprod., 2, van der Ven K., Peschka B., Lange R. et al. (1998) Increased frequency of constitutional chromosomal aberrations in female partners of couples undergoing intracytoplasmic sperm injection (ICSI). Hum. Reprod., 13, in press. Wall, M.B., Marks, K., Smith, T.A. et al. (1996) Cytogenetic and fluorescent in-situ hybridization chromosomal studies on in-vitro fertilized and intracytoplasmic sperm injected failed-fertilized human oocytes. Hum. Reprod., 11, Wramsby, H., Fredga, K. and Liedholm, P. (1987) Chromosome analysis of human oocytes recovered from preovulatory follicles in stimulated cycles. N. Engl. J. Med., 316, Received on March 7, 1997; accepted on August 22,
IVF AND PREIMPLANTATION GENETIC TESTING FOR ANEUPLOIDY (PGT-A) WHAT THE COMMUNITY PHYSICIAN NEEDS TO KNOW
IVF AND PREIMPLANTATION GENETIC TESTING FOR ANEUPLOIDY (PGT-A) WHAT THE COMMUNITY PHYSICIAN NEEDS TO KNOW Jon Havelock, MD, FRCSC, FACOG Co-Director - PCRM Disclosure No conflict of interest in relation
More informationFertility of ejaculated and testicular megalohead spermatozoa with intracytoplasmic sperm injection
Human Reproduction vol.14 no.3 pp.726 730, 1999 Fertility of ejaculated and testicular megalohead spermatozoa with intracytoplasmic sperm injection S.Kahraman 1,4, C.Akarsu 1, G.Cengiz 1, K.Dirican 1,
More informationP.M.M.Kastrop 1, S.M.Weima, R.J.Van Kooij and E.R.Te Velde
Human Reproduction vol.14 no.1 pp.65 69, 1999 Comparison between intracytoplasmic sperm injection and in-vitro fertilization (IVF) with high insemination concentration after total fertilization failure
More informationChromosome translocations in couples with in-vitro fertilization implantation failure
Human Reproduction vol.14 no.8 pp.2097 2101, 1999 Chromosome translocations in couples with in-vitro fertilization implantation failure C.Stern 1,4, M.Pertile 2, H.Norris 1, L.Hale 1 and H.W.G.Baker 3
More informationInfertility treatment
In the name of God Infertility treatment Treatment options The optimal treatment is one that provide an acceptable success rate, has minimal risk and is costeffective. The treatment options are: 1- Ovulation
More informationH.Van de Velde 1, Z.P.Nagy, H.Joris, A.De Vos and A.C.Van Steirteghem
Human Reproduction vol.12 no.10 pp.2246 2250, 1997 Effects of different hyaluronidase concentrations and mechanical procedures for cumulus cell removal on the outcome of intracytoplasmic sperm injection
More informationS.Kahraman 1,4, M.Bahçe 2,H.Şamlı 3, N.İmirzalıoğlu 2, K.Yakısn 1, G.Cengiz 1 and E.Dönmez 1
Human Reproduction vol.15 no.9 pp.2003 2007, 2000 Healthy births and ongoing pregnancies obtained by preimplantation genetic diagnosis in patients with advanced maternal age and recurrent implantation
More informationPreimplantation genetic diagnosis: polar body and embryo biopsy
Human Reproduction, Vol. 15, (Suppl. 4), pp. 69-75, 2000 Preimplantation genetic diagnosis: polar body and embryo biopsy Luca Gianaroli SISMER, Via Mazzini 12, 40138 Bologna, Italy Scientific Director
More informationArticles Impact of parental gonosomal mosaicism detected in peripheral blood on preimplantation embryos
RBMOnline - Vol 5. No 3. 306 312 Reproductive BioMedicine Online; www.rbmonline.com/article/699 on web 12 September Articles Impact of parental gonosomal mosaicism detected in peripheral blood on preimplantation
More informationSynchronization between embryo development and endometrium is a contributing factor for rescue ICSI outcome
Reproductive BioMedicine Online (2012) 24, 527 531 www.sciencedirect.com www.rbmonline.com ARTICLE Synchronization between embryo development and endometrium is a contributing factor for rescue ICSI outcome
More informationThe study of relationship between chromosomal abnormality in lymphocyte cells of infertile men with intra-cytoplasmic sperm injection outcomes
The study of relationship between chromosomal abnormality in lymphocyte cells of infertile men with intra-cytoplasmic sperm injection outcomes Fallahi P, Rezaeian Z, *Moghbelinejad S Fertility and infertility
More informationArticle Influence of spermatogenic profile and meiotic abnormalities on reproductive outcome of infertile patients
RBMOnline - Vol 10. No 6. 2005 735 739 Reproductive BioMedicine Online; www.rbmonline.com/article/1678 on web 13 April 2005 Article Influence of spermatogenic profile and meiotic abnormalities on reproductive
More informationAssisted Reproduction. By Dr. Afraa Mahjoob Al-Naddawi
Assisted Reproduction By Dr. Afraa Mahjoob Al-Naddawi Learning Objectives: By the end of this lecture, you will be able to: 1) Define assisted reproductive techniques (ART). 2) List indications for various
More informationUnderstanding eggs, sperm and embryos. Marta Jansa Perez Wolfson Fertility Centre
Understanding eggs, sperm and embryos Marta Jansa Perez Wolfson Fertility Centre What does embryology involve? Aims of the embryology laboratory Creation of a large number of embryos and supporting their
More informationCandido Tomás 1,3, Mauri Orava 1, Leena Tuomivaara 2 and Hannu Martikainen 1
Human Reproduction vol.13 no.1 pp. 65 70, 1998 Low pregnancy rate is achieved in patients treated with intracytoplasmic sperm injection due to previous low or failed fertilization in in-vitro fertilization
More informationGenetics Aspects of Male infertility
Genetics Aspects of Male infertility A. Ebrahimi, Molecular Genetic SM Kalantar, Prof. Molecular Cytogenetic Research & Clinical Centre for Infertility, Reproductive & Genetic Unit, Yazd Medical Sciences
More informationPreimplantation Genetic Testing
Protocol Preimplantation Genetic Testing (40205) Medical Benefit Effective Date: 01/01/14 Next Review Date: 09/14 Preauthorization No Review Dates: 09/11, 09/12, 09/13 The following Protocol contains medical
More informationExtended sperm preparation: an alternative to testicular sperm extraction in non-obstructive azoospermia
Human Reproduction vol.12 no.6 pp.1222 1226, 1997 Extended sperm preparation: an alternative to testicular sperm extraction in non-obstructive azoospermia R.Ron-El 1, D.Strassburger, S.Friedler, D.Komarovski,
More informationFertilization failures and abnormal fertilization after intracytoplasmic sperm injection
Fertilization failures and abnormal fertilization after intracytoplasmic sperm injection Sean P.Flaherty 1, Dianna Payne and Colin D.Matthews Reproductive Medicine Unit, Department of Obstetrics and Gynaecology,
More informationAbstract. Introduction
RBMOnline - Vol 13 No 6. 2006 869-874 Reproductive BioMedicine Online; www.rbmonline.com/article/2507 on web 18 October 2006 Article Preimplantation genetic diagnosis significantly improves the pregnancy
More informationSNP array-based analyses of unbalanced embryos as a reference to distinguish between balanced translocation carrier and normal blastocysts
J Assist Reprod Genet (2016) 33:1115 1119 DOI 10.1007/s10815-016-0734-0 TECHNOLOGICAL INNOVATIONS SNP array-based analyses of unbalanced embryos as a reference to distinguish between balanced translocation
More informationIncidence of Chromosomal Abnormalities from a Morphologically Normal Cohort of Embryos in Poor- Prognosis Patients
Incidence of Chromosomal Abnormalities from a Morphologically Normal Cohort of Embryos in Poor- Prognosis Patients M. C. MAGLI,1 L. GIANAROLI,1,3 S. MUNNE,2 and A. P. FERRARETTI1 Submitted: December 29,
More informationChromosomal aberrations in couples undergoing intracytoplasmic sperm injection: influence on implantation and ongoing pregnancy rates
FERTILITY AND STERILITY VOL. 70, NO. 5, NOVEMBER 1998 Copyright 1998 American Society for Reproductive Medicine Published by Elsevier Science Inc. Printed on acid-free paper in U.S.A. Chromosomal aberrations
More informationChromosomal Structural Abnormalities among Filipino Couples with Recurrent Pregnancy Losses
ORIGINAL CASE REPORT ARTICLE Chromosomal Structural Abnormalities among Filipino Couples with Recurrent Pregnancy Losses Eva Maria Cutiongco-dela Paz,,2 April Grace Dion-Berboso, Edsel Allan G. Salonga
More informationCommittee Paper SCAAC(05/09)01. ICSI guidance. Hannah Darby and Rachel Fowler
Committee Paper Committee: Scientific and Clinical Advances Advisory Committee Meeting Date: 12 May 2009 Agenda Item: 4 Paper Number: SCAAC(05/09)01 Paper Title: ICSI guidance Author: Hannah Darby and
More informationIN VITRO FERTILIZATION
FERTILITY AND STERILITY VOL. 78, NO. 3, SEPTEMBER 2002 Copyright 2002 American Society for Reproductive Medicine Published by Elsevier Science Inc. Printed on acid-free paper in U.S.A. IN VITRO FERTILIZATION
More informationINDICATIONS OF IVF/ICSI
PROCESS OF IVF/ICSI INDICATIONS OF IVF/ICSI IVF is most clearly indicated when infertility results from one or more causes having no other effective treatment; Tubal disease. In women with blocked fallopian
More informationPOST - DOCTORAL FELLOWSHIP PROGRAMME IN REPRODUCTIVE MEDICINE. Anatomy : Male and Female genital tract
POST - DOCTORAL FELLOWSHIP PROGRAMME IN REPRODUCTIVE MEDICINE DURATION OF THE COURSE : TWO YEARS Detailed syllabus: Part 1 Basic Sciences: Anatomy : Male and Female genital tract Physiology Endocrinology
More informationChromosome Abnormalities
Chromosome Abnormalities Chromosomal abnormalities vs. molecular mutations Simply a matter of size Chromosomal abnormalities are big errors Two types of abnormalities 1. Constitutional problem present
More informationReproductive Technology, Genetic Testing, and Gene Therapy
Michael Cummings Chapter 16 Reproductive Technology, Genetic Testing, and Gene Therapy David Reisman University of South Carolina 16.1 Infertility Is a Common Problem In the US, about 13% of all couples
More informationAbstract. Introduction. Materials and methods. Patients and methods
RBMOnline - Vol 8. No 3. 344-348 Reproductive BioMedicine Online; www.rbmonline.com/article/1178 on web 20 January 2004 Article Cumulative live birth rates after transfer of cryopreserved ICSI embryos
More informationCYTOGENETICS Dr. Mary Ann Perle
CYTOGENETICS Dr. Mary Ann Perle I) Mitosis and metaphase chromosomes A) Chromosomes are most fully condensed and clearly distinguishable during mitosis. B) Mitosis (M phase) takes 1 to 2 hrs and is divided
More informationInduction of the human sperm acrosome reaction by human oocytes*
FERTILITY AND STERILITY Copyright C> 1988 The American Fertility Society Vol. 50, No.6, December 1988 Printed in U.S.A. Induction of the human sperm acrosome reaction by human oocytes* Christopher J. De
More informationInternational Journal of Technical Research and Applications e Milat Ismail Haje1, Kameel M Naoom2
THE EFFECT OF SPERM PARAMETERS AND BOTH MATERNAL AND PATERNAL AGE ON OUTCOME OF INTRACYTOPLASMIC SPERM INJECTION Milat Ismail Haje 1, Kameel M Naoom 2 1 Postgraduate student in College of Medicine, Hawler
More informationCopyright 1995 American Society for Reproductive Medicine
FERTILITY AND STERILITY Vol. 64, No.6, December 1995 Copyright 1995 American Society for Reproductive Medicine Printed on acid free paper in U. S. A. Prospective, auto-controlled study on reinsemination
More informationAgonist versus antagonist in ICSI cycles: a randomized trial and cost effectiveness analysis Badrawi A, Zaki S, Al-Inany H, Ramzy A M, Hussein M
Agonist versus antagonist in ICSI cycles: a randomized trial and cost effectiveness analysis Badrawi A, Zaki S, Al-Inany H, Ramzy A M, Hussein M Record Status This is a critical abstract of an economic
More informationIN VITRO FERTILISATION (IVF)
IN VITRO FERTILISATION (IVF) Pre Treatment - first cycle 785 Medical Consultation 225 Nurse Planning 235 Baseline ultrasound scan of uterus and ovaries HIV, Hep B antibodies, Hep B antigen, Hep C blood
More informationPredictive factors of successful pregnancy after assisted reproductive technology in women aged 40 years and older
Reprod Med Biol (2009) 8:145 149 DOI 10.1007/s12522-009-0023-z ORIGINAL ARTICLE Predictive factors of successful pregnancy after assisted reproductive technology in women aged 40 years and older Akihisa
More informationBiology of fertility control. Higher Human Biology
Biology of fertility control Higher Human Biology Learning Intention Compare fertile periods in females and males What is infertility? Infertility is the inability of a sexually active, non-contracepting
More informationProblem Challenge Need. Solution Innovation Invention
Problem Challenge Need Solution Innovation Invention Tubal Infertility In-vitro Fertilisation Steptoe and Edwards Birth after the reimplantation of a human embryo. Lancet 1978 Louise Brown, 25. Juli 1978
More informationRejuvenation of Gamete Cells; Past, Present and Future
Rejuvenation of Gamete Cells; Past, Present and Future Denny Sakkas PhD Scientific Director, Boston IVF Waltham, MA, USA Conflict of Interest I have no conflict of interest related to this presentation.
More informationFertility 101. About SCRC. A Primary Care Approach to Diagnosing and Treating Infertility. Definition of Infertility. Dr.
Dr. Shahin Ghadir A Primary Care Approach to Diagnosing and Treating Infertility St. Charles Bend Grand Rounds November 30, 2018 I have no conflicts of interest to disclose. + About SCRC State-of-the-art
More informationSherman J.Silber 1,3, Zsolt Nagy 2, Paul Devroey 2, Michel Camus 2 and André C.Van Steirteghem 2
Human Reproduction vol.12 no.12 pp.2693 2700, 1997 The effect of female age and ovarian reserve on pregnancy rate in male infertility: treatment of azoospermia with sperm retrieval and intracytoplasmic
More informationChromosome pathology
Chromosome pathology S. Dahoun Department of Gynecology and Obstetrics, University Hospital of Geneva Cytogenetics is the study of chromosomes and the related disease states caused by abnormal chromosome
More informationEasily decapitated spermatozoa defect: a possible cause of unexplained infertility
Human Reproduction vol.4 no. pp.79-795, 999 Easily decapitated spermatozoa defect: a possible cause of unexplained infertility A.Kamal, R.Mansour, I.Fahmy, G.Serour, C.Rhodes and M.Aboulghar,3 The Egyptian
More informationIVF (,, ) : (HP-hMG) - (IVF- ET) : GnRH, HP-hMG (HP-hMG )57, (rfsh )140, (Gn)
34 11 Vol.34 No.11 2014 11 Nov. 2014 Reproduction & Contraception doi: 10.7669/j.issn.0253-3X.2014.11.0892 E-mail: randc_journal@163.com IVF ( 710003) : (H-hMG) - (IVF- ET) : GnRH H-hMG (H-hMG ) (rfsh
More informationPre-implantation genetic diagnosis and pre-implantation genetic screening: two years experience at a single center
Original Article Obstet Gynecol Sci 2018;61(1):95-101 https://doi.org/10.5468/ogs.2018.61.1.95 pissn 2287-8572 eissn 2287-8580 Pre-implantation genetic diagnosis and pre-implantation genetic screening:
More informationClinical Policy Committee
Northern, Eastern and Western Devon Clinical Commissioning Group South Devon and Torbay Clinical Commissioning Group Clinical Policy Committee Commissioning policy: Assisted Conception Fertility treatments
More informationIncidence and development of zygotes exhibiting abnormal pronuclear disposition after identification of two pronuclei at the fertilization check
Incidence and development of zygotes exhibiting abnormal pronuclear disposition after identification of two pronuclei at the fertilization check David E. Reichman, M.D., Katharine V. Jackson, B.A., and
More informationMenstruation-free interval and ongoing pregnancy in IVF using GnRH antagonists
Human Reproduction Vol.21, No.4 pp. 1012 1017, 2006 Advance Access publication December 8, 2005. doi:10.1093/humrep/dei415 Menstruation-free interval and ongoing pregnancy in IVF using GnRH antagonists
More informationMSOME I+II: A NEW CUT-OFF VALUE FOR MALE INFERTILITY AND EMBRYO DEVELOPMENT PREDICTION ON INTRACYTOPLASMIC SPERM INJECTION CYCLES
MSOME I+II: A NEW CUT-OFF VALUE FOR MALE INFERTILITY AND EMBRYO DEVELOPMENT PREDICTION ON INTRACYTOPLASMIC SPERM INJECTION CYCLES Edson Borges Jr 1,2, ; Bianca Ferrarini Zanetti 1,2, Daniela Paes de Almeida
More informationChromosomal abnormalities in infertile men and preimplantation embryos Dul, Elsbeth
University of Groningen Chromosomal abnormalities in infertile men and preimplantation embryos Dul, Elsbeth IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish
More informationComparison between day-2 embryos obtained either from ICSI or resulting from short insemination IVF: influence of maternal age*
Human Reproduction vol.15 no.8 pp.1776 1780, 2000 Comparison between day-2 embryos obtained either from ICSI or resulting from short insemination IVF: influence of maternal age* Yves Ménézo 1,3 and Yona
More informationAbstract. Introduction. Materials and methods
RBMOnline - Vol 10. No 5. 2005 645 649 Reproductive BioMedicine Online; www.rbmonline.com/article/1518 on web 18 March 2005 Article Factors predicting IVF treatment outcome: a multivariate analysis of
More informationRelation between the Number and Size of Follicles in Ovulation Induction and the Rate of Pregnancy
Relation between the Number and Size of Follicles in Ovulation Induction and the Rate of Pregnancy Aseel Mosa Jabber M.SC.G.O. The department of Obstetrics and Gynecology, Faculty of Medicine Thi-qar university
More informationThe paternal effect of chromosome translocation carriers observed from meiotic segregation in embryos
Human Reproduction, Vol.25, No.7 pp. 1843 1848, 2010 Advanced Access publication on May 28, 2010 doi:10.1093/humrep/deq111 ORIGINAL ARTICLE Reproductive genetics The paternal effect of chromosome translocation
More informationK.W.Fuh, X.Wang, A.Tai, I.Wong and R.J.Norman 1
Human Reproduction vol.12 no.10 pp.2162 2166, 1997 Intrauterine insemination: effect of the temporal relationship between the luteinizing hormone surge, human chorionic gonadotrophin administration and
More informationAdoption and Foster Care
GLOSSARY Family building via Adoption and Foster Care October 2018 www.familyequality.org/resources A Anonymous Donor: A person who donated sperm or eggs with the intention of never meeting resulting children.
More informationStructural chromosomal abnormalities in couples in cases of recurrent spontaneous abortions in Jilin Province, China
Structural chromosomal abnormalities in couples in cases of recurrent spontaneous abortions in Jilin Province, China H.-T. Fan, M. Zhang, P. Zhan, X. Yang, W.-J. Tian and R.-W. Li Andrology Laboratory,
More informationNICE fertility guidelines. Hemlata Thackare MPhil MSc MRCOG Deputy Medical Director London Women s Clinic
NICE fertility guidelines Hemlata Thackare MPhil MSc MRCOG Deputy Medical Director London Women s Clinic About the LWC 4 centres around the UK London Cardiff Swansea Darlington The largest sperm bank in
More informationOriginal Policy Date
MP 2.04.77 Preimplantation Genetic Testing Medical Policy Section OB/Gyn/Reproduction Issue 12:2013 Original Policy Date 12:2013 Last Review Status/Date Reviewed with literature search/12:2013 Return to
More informationUnderstanding the Human Karyotype Colleen Jackson Cook, Ph.D.
Understanding the Human Karyotype Colleen Jackson Cook, Ph.D. SUPPLEMENTAL READING Nussbaum, RL, McInnes, RR, and Willard HF (2007) Thompson and Thompson Genetics in Medicine, 7th edition. Saunders: Philadelphia.
More informationA Retrospective Cytogenetic Study of Chromosomal Abnormalities in Infertile Couples of Indian Origin
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2017, 9 [4]:44-56 [http://scholarsresearchlibrary.com/archive.html] ISSN 0975-5071 USA CODEN: DPLEB4
More informationStudy on Several Factors Involved in IVF-ET of Human Beings
Study on Several Factors Involved in IVF-ET of Human Beings Lei X 1, Zhuoran W 1, Bin L 1, Huiming L 1, Hongxiu Z 1, Yajuan Z 1, Yingbo Q 1, Guixue Z 2 1 The First Clinical College of Harbin Medical University,
More informationFemale Reproductive Physiology. Dr Raelia Lew CREI, FRANZCOG, PhD, MMed, MBBS Fertility Specialist, Melbourne IVF
Female Reproductive Physiology Dr Raelia Lew CREI, FRANZCOG, PhD, MMed, MBBS Fertility Specialist, Melbourne IVF REFERENCE Lew, R, Natural History of ovarian function including assessment of ovarian reserve
More information% Oocyte Donation Pregnancyes (days 3)
Ovulation induction in oocyte donors Roma- September 2007 Dr. José Remohí Dr. Carmen Rubio Dr. Amparo Mercader Dr. Pilar Alama Dr. Marco Melo Evolution of oocyte donation cycles 1500 1500 1000 58% 661
More informationIntracytoplasmic Sperm Injection Outcome Using Ejaculated Sperm and Retrieved Sperm in Azoospermic Men
Sexual Dysfunction and Infertility Intracytoplasmic Sperm Injection Outcome Using Ejaculated Sperm and Retrieved Sperm in Azoospermic Men Tahira Naru, 1 M Nasir Sulaiman, 2 Atiya Kidwai, 3 M Hammad Ather,
More informationAssisted reproductive technology
Assisted reproductive technology FERTILITY AND STERILITY Copyright 1994 The American Fertility Society Vol. 62, No.4, October 1994 Printed on acid-free paper in U. S. A. Cryopreservation of supernumerary
More informationDual color fluorescence in situ hybridization to investigate aneuploidy in sperm from 33 normal males and a man with a t(2;4;8)(q23;q27; p21)*
FERTILITY AND STERILITY Copyright" 1994 The American Fertility Society Printed on acid-free paper in U. S. A. Dual color fluorescence in situ hybridization to investigate aneuploidy in sperm from 33 normal
More informationKersti Lundin 1, Brita Söderlund and Lars Hamberger
Human Reproduction vol.12 no.12, pp.2676 2681, 1997 The relationship between sperm morphology and rates of fertilization, pregnancy and spontaneous abortion in an in-vitro fertilization/intracytoplasmic
More informationIndications for chromosome screening Dagan Wells, PhD, FRCPath dagan.wells@obs-gyn.ox.ac.ukgyn.ox.ac.uk Chromosome imbalance (aneuploidy) Uncontroversial data The incidence of aneuploidy Aneuploidy is
More informationEffects of triploidy after intracytoplasmic sperm injection on in vitro fertilization cycle outcome
Effects of triploidy after intracytoplasmic sperm injection on in vitro fertilization cycle outcome Molina B. Dayal, M.D., M.P.H., Paul R. Gindoff, M.D., Abbaa Sarhan, M.D., Anil Dubey, Ph.D., Douglas
More informationBenefit of intracytoplasmic sperm injection in patients with a high incidence of triploidy in a prior in vitro fertilization cycle
IN VITRO FERTILIZATION Benefit of intracytoplasmic sperm injection in patients with a high incidence of triploidy in a prior in vitro fertilization cycle Sunny H. Jun, M.D., a Thomas O Leary, B.S., b Katharine
More informationThe benefit of artificial oocyte activation is dependent on the fertilization rate in a previous treatment cycle
Reproductive BioMedicine Online (2012) 24, 521 526 www.sciencedirect.com www.rbmonline.com ARTICLE The benefit of artificial oocyte activation is dependent on the fertilization rate in a previous treatment
More informationSubmitted April 2, 2003; accepted June 5, KEY WORDS: Fertility; fertilization; oocyte; reproductive techniques. INTRODUCTION
( C 2003) Assisted Reproduction Estimation of Second Polar Body Retention Rate After Conventional Insemination and Intracytoplasmic Sperm Injection: In Vitro Observations From more than 5000 Human Oocytes
More informationInfluence ovarian stimulation on oocyte and embryo quality. Prof.Dr. Bart CJM Fauser
Influence ovarian stimulation on oocyte and embryo quality Prof.Dr. Bart CJM Fauser How to balance too much vs too little? Lecture Outline Context ovarian stimulation Impact ovarian stimulation on oocyte
More informationPaternal effects acting during the first cell cycle of human preimplantation development after ICSI
Human Reproduction Vol.17, No.1 pp. 184 189, 2002 Paternal effects acting during the first cell cycle of human preimplantation development after ICSI Jan Tesarik 1,2,5, Carmen Mendoza 2,3 and Ermanno Greco
More informationLuteal phase rescue after GnRHa triggering Progesterone and Estradiol
Luteal phase rescue after GnRHa triggering Progesterone and Estradiol L. Engmann University of Connecticut Disclaimer Fertility Speaker Bureau Merck Pharmaceuticals Introduction GnRH agonist is effective
More informationLOW RESPONDERS. Poor Ovarian Response, Por
LOW RESPONDERS Poor Ovarian Response, Por Patients with a low number of retrieved oocytes despite adequate ovarian stimulation during fertility treatment. Diagnosis Female About Low responders In patients
More informationThe work of a fertility specialist Steven Fleming PhD Honorary Associate, University of Sydney Director of Embryology, ORIGIO a/s
The work of a fertility specialist Steven Fleming PhD Honorary Associate, University of Sydney Director of Embryology, ORIGIO a/s sfleming@origio.com Scope of work Evaluation and diagnosis of the infertile
More informationSperm Function Tests Beyond the Semen Analysis
Sperm Function Tests Beyond the Semen Analysis Edmund Sabanegh, Jr., M.D. Chairman, Department of Urology Glickman Urological and Kidney Institute Cleveland Clinic Routine Semen Analysis: Gap in Knowledge?
More informationOocyte maturation. A.Trounson 1 ' 3, C.Anderiesz 1, G.MJones 1, A.Kausche 1, N.Lolatgis 2 and C.Wood 2
A.Trounson 1 ' 3, C.Anderiesz 1, G.MJones 1, A.Kausche 1, N.Lolatgis 2 and C.Wood 2 Centre for Early Human Development, Institute of Reproduction and Development, Monash University, Monash Medical Centre,
More informationConsultations and Assessment Fertility Specialist consultation 180 Ultrasound scan of uterus and ovaries 100 AMH measurement 80 Semen analysis 100
We hope this price list will help you assess the cost of your consultations, investigations and treatment. It provides information about what is included in the cost and how we make a refund if your treatment
More informationPatient Price List. t: e: w:
Patient Price List t: 0333 015 9774 e: enquires@ivi.uk w: www.ivi.uk fertility treatments Pre Treatment Medical Consultation 250 Nurse Planning 200 Baseline ultrasound scan of uterus and ovaries included
More informationShould we advise patients undergoing IVF to start a cycle leading to a day 3 or a day 5 transfer?
Human Reproduction Vol.19, No.11 pp. 2550 2554, 2004 Advance Access publication August 6, 2004 doi:10.1093/humrep/deh447 Should we advise patients undergoing IVF to start a cycle leading to a day 3 or
More informationPrenatal testing in ICSI pregnancies: incidence of chromosomal anomalies in 1586 karyotypes and relation to sperm parameters
Human Reproduction Vol.17, No.10 pp. 2600 2614, 2002 Prenatal testing in ICSI pregnancies: incidence of chromosomal anomalies in 1586 karyotypes and relation to sperm parameters Maryse Bonduelle 1,3, Elvire
More informationAbstract. Introduction. RBMOnline - Vol 19. No Reproductive BioMedicine Online; on web 24 August 2009
RBMOnline - Vol 19. No 4. 2009 599 603 Reproductive BioMedicine Online; www.rbmonline.com/article/3872 on web 24 August 2009 Article Assisted reproduction in women over 40 years of age: how old is too
More informationCase report Successful pregnancy after ICSI with strontium oocyte activation in low rates of fertilization
RBMOnline - Vol 13 No 6. 2006 801-806 Reproductive BioMedicine Online; www.rbmonline.com/article/2369 on web 19 October 2006 Case report Successful pregnancy after ICSI with strontium oocyte activation
More informationWOMEN & INFANTS HOSPITAL Providence, RI CONSENT FOR IVF WITH EMBRYO TRANSFER
*40639* 40639 WOMEN & INFANTS HOSPITAL Providence, RI 02905 CONSENT FOR IVF WITH EMBRYO TRANSFER I have requested treatment by the physicians and (Print Patient s name) staff of the Women & Infants Fertility
More informationPredictive value of embryo grading for embryos with known outcomes
Predictive value of embryo grading for embryos with known outcomes Vanessa N. Weitzman, M.D., Jennifer Schnee-Riesz, M.D., Claudio Benadiva, M.D., John Nulsen, M.D., Linda Siano, M.S., and Donald Maier,
More informationShould cryopreserved epididymal or testicular sperm be recovered from obstructive azoospermic men for ICSI?
BJOG: an International Journal of Obstetrics and Gynaecology November 2004, Vol. 111, pp. 1289 1293 DOI: 10.1111/j.1471-0528.2004.00411.x Should cryopreserved epididymal or testicular sperm be recovered
More informationOptimize your success
ANDROLOGY BY ORIGIO Optimize your success by selecting the highest quality sperm QUALITY CONTROL OOCYTE RETRIEVAL ANDROLOGY & IUI FERTILIZATION CULTURE PGD & PGS CRYOPRESERVATION EMBRYO TRANSFER The importance
More informationFailed fertilization after clinical intracytoplasmic sperm injection
Failed fertilization after clinical intracytoplasmic sperm injection Reproductive BioMedicine Online 2009 Vol. 19 No.2. 216 220 Present by R4 郭恬妮 Introduction intracytoplasmic sperm injection (ICSI) choice
More informationA Retrospective Study of Balanced Chromosomal Translocations in a Turkish Population
Kamla-Raj 2012 Int J Hum Genet, 12(4): 319-323 (2012) A Retrospective Study of Balanced Chromosomal Translocations in a Turkish Population N. Karakus 1, N. Kara 1, S. Tural 1, I. Kocak 2 and M. Elbistan
More informationFAILED OOCYTE MATURATION. A Fekih, N Farah, D Chardonnens, F Urner, D De Ziegler, PG Bianchi, P Mock, A Campana, H Lucas
FAILED OOCYTE MATURATION A Fekih, N Farah, D Chardonnens, F Urner, D De Ziegler, PG Bianchi, P Mock, A Campana, H Lucas INTRODUCTION In our laboratory,we perform (X) IVF and (Y) ICSI per year with a success
More informationChromosomal Aneuploidy
The Many Advantages of Trophectoderm Biopsy Compared to Day 3 Biopsy for Pre- Implantation Genetic Screening (PGS) Mandy Katz-Jaffe, PhD Chromosomal Aneuploidy Trisomy 21 Fetus Aneuploidy is the most common
More informationI. ART PROCEDURES. A. In Vitro Fertilization (IVF)
DFW Fertility Associates ASSISTED REPRODUCTIVE TECHNOLOGY (ART) Welcome to DFW Fertility Associates/ Presbyterian-Harris Methodist Hospital ARTS program. This document provides an overview of treatment
More informationPreimplantation Genetic Testing Where are we going? Genomics Clinical Medicine Symposium Sept 29,2012 Jason Flanagan, MS,CGC
Preimplantation Genetic Testing Where are we going? Genomics Clinical Medicine Symposium Sept 29,2012 Jason Flanagan, MS,CGC Overview Discuss what PGD and PGS are Pt examples What we have learned Where
More information