The Magic Erase-her The Erase Sperm Isolation Kit in DNA Casework. Shena Latcham Missouri State Highway Patrol Crime Lab Jefferson City
|
|
|
- Dayna Potter
- 6 years ago
- Views:
Transcription
1 The Magic Erase-her The Erase Sperm Isolation Kit in DNA Casework Shena Latcham Missouri State Highway Patrol Crime Lab Jefferson City
2 Meet the Erase Sperm Isolation Kit Purpose: More effectively and efficiently separate samples containing mixtures of sperm cells and epithelial cells into their respective components HOW??????? Use a DNase to chew up epithelial cells remaining in the sperm fraction without destroying the sperm cells Developed and distributed by the Paternity Testing Corporation, Columbia, MO
3 Why Should We Validate Erase? Easy to use Comes in a pre-made kit No reagent preparation Quicker Large sexual assault backlog ½ the time of an organic differential ~2 hr. hands-on ~1.5 hr. organic clean-up NO SPERM WASHES!!!!! Single-source male profiles in the sperm fractions
4 Validation on the Maxwell 16 LEV Let s be even MORE efficient! Clean-up on the Maxwell 16 LEV <30 min clean-up No filtration necessary (decreased inhibition) No PCI involved 40 samples with various.. Substrates Concentrations Body fluids Post-coital time intervals
5 Implementation Validation completed September 24, 2012 Competencies October November 2012 Proficiencies November December 2012 First used on case work November 13, 2012
6 In the Beginning. Everything Went Great!
7 Case #1: Sperm Fraction
8 Case #1: Non-sperm Fraction
9 And Then. The Honeymoon Was Over
10 What s Happening to Our Data?! Sperm fractions Decline in singlesource male profiles Increase in stops at quant samples Obtained female profiles in the sperm fraction Non-sperm fractions appeared unaffected Maybe some diminished peak heights
11 The Culprit: Inadequate Kit Storage New kits originally stored at a satellite lab Kits stored in a frost-free freezer for months Should be stored at -80 (ideal) Not discovered for ~1 month Precipitate in solution 3 19 samples affected Obtained new kit from PTC Data was BEAUTIFUL again!
12 Types of Profiles Obtained (Overall) 55 samples tested to date (110 fractions) Various substrates and times post-coital Sperm fractions: mostly single-source male Non-sperm fractions: male, female, and mixtures
13 Breaking Down the Data: Sperm Fractions (Overall) Profiles obtained from 62% of samples tested 79% of these were single-source male 36% of samples stopped at quant 13 different cases affected (20 samples) All but 4 samples could be explained PSA cards too sensitive (no sperm cells detected) Multiple samples in same case stopped at quant (vasectomy?) Extracted with the problem kit
14 Types of Profiles Obtained (Without Bad Kit Data) 36 samples tested (72 fractions) Sperm fractions: predominately single-source male Non-sperm fractions: male, female, and mixtures (no change from before)
15 Breaking Down the Data: Sperm Fractions (w/o Bad Kit Data) Profiles obtained from 69% of samples tested 84% of these were single-source male 30% of samples stopped at quant 11 samples affected (8 cases) 7 samples: cases with multiple samples that all stopped at quant Possible vasectomy? 2 samples: PSA test too sensitive not truly seminal fluid?
16 Current Case: Sperm Fraction Male on Male Sexual Assault
17 Current Case: Non-sperm Fraction Male on Male Sexual Assault
18 Sperm Cell Detection Sample removed from isolated sperm fraction After aspirating off the non-sperm fraction Prior to addition of solutions 1 and uL heat fixed to slide Stained with KPIC Visualized microscopically with a 20X or 40X lens
19 WE LOVE IT!! Faster and easier Summary Traditional organic differential - last resort Bringing satellite lab on-line now Some cons Dolphin nose tubes Once you add solutions 1 and 2, you CANNOT walk away from samples CANNOT stop at extraction; must continue through isolation
20 Paternity Testing Corporation Thank you!! MSHP Crime Laboratory -Jefferson City AFDAA
Independent Forensics 4600 West Roosevelt Road Suite 201 Hillside IL 60162, USA. Telephone: (708) Fax: (708)
Developmental validation of SPERM HY-LITER A Specific, Sensitive and Confirmatory Microscopic Screening Method for the Detection of Human Sperm from Sexual Assault Evidence Independent Forensics 4600 West
1.0 Purpose - This procedure specifies the method for conducting analysis for semen and sperm in forensic casework.
Procedure for Semen and Sperm Analysis 1.0 Purpose - This procedure specifies the method for conducting analysis for semen and sperm in forensic casework. 2.0 Scope - This procedure applies to those Forensic
DEVELOPMENTAL VALIDATION OF SPERM HY-LITER EXPRESS Jennifer Old, Chris Martersteck, Anna Kalinina, Independent Forensics, Lombard IL
DEVELOPMENTAL VALIDATION OF SPERM HY-LITER EXPRESS Jennifer Old, Chris Martersteck, Anna Kalinina, Independent Forensics, Lombard IL Background and Introduction SPERM HY-LITER is the first immunofluorescent
SPERM HY-LITER TM. Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual Assault Evidence. Jack Keehma Austin TX February 1, 2008
Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual Assault Evidence Jack Keehma Austin TX February 1, 2008 Introduction to Independent Forensics Forensic DNA laboratory Accreditations:
INSTRUCTION MANUAL. RNA Clean & Concentrator -5 Catalog Nos. R1015 & R1016. Highlights. Contents
INSTRUCTION MANUAL Catalog Nos. R1015 & R1016 Highlights Quick (5 minute) method for cleaning and concentrating RNA. Ideal for purification of RNA from aqueous phase following an acid phenol extraction.
Differential extraction using Alkaline Lysis and Pressure cycling
Differential extraction using Alkaline Lysis and Pressure cycling Deepthi Nori and Bruce McCord Department of Chemistry and Biochemistry Florida International University, Miami, FL mccordb@fiu.edu Issues
Application of Pressure Cycling Technology (PCT) in Differential Extraction
Application of Pressure Cycling Technology (PCT) in Differential Extraction Deepthi Nori, MFS; Dr. Bruce R. McCord, PhD Department of Chemistry International Forensic Research Institute Florida International
Shawn Montpetit, DNA Technical Manager San Diego Police Department Crime Laboratory
Shawn Montpetit, DNA Technical Manager San Diego Police Department Crime Laboratory 2014 Qiagen North American User s Group Meeting Houston, TX 2014 QIAGEN would like to thank our speaker, Shawn Montpetit,
Unit 7: Blood/ Serology
Unit 7: Blood/ Serology Forensic Serology the examination and analysis of body fluids including: saliva semen urine Blood 1950-1980 s, forensic serology = most important part of lab procedures (until DNA).
3. What is used to test for the presence of simple reducing sugars such as monosaccharides?
Lab 7-1 Analysis and s 1. What is used to test for the presence of starch? 2. How can you tell by using this test that a substance contains starch? 3. What is used to test for the presence of simple reducing
DNA TECHNIQUES AVAILABLE FOR USE IN FORENSIC CASE WORK
DNA TECHNIQUES AVAILABLE FOR USE IN FORENSIC CASE WORK FOR FORENSIC ASSISTANCE 24 HOURS A DAY, 7 DAYS A WEEK 0800 FORENSIC 0800 367 367 z KEY CONTACT Dr Stephen Cordiner Forensic Manager Service Centres
For Research Use Only Ver
INSTRUCTION MANUAL Quick-RNA Miniprep Kit Catalog Nos. R1054 & R1055 Highlights High-quality total RNA (including small RNAs) from a wide range of samples. You can opt to isolate small and large RNAs in
For Research Use Only Ver
INSTRUCTION MANUAL Quick-RNA Miniprep Kit Catalog Nos. R1054 & R1055 Highlights High-quality total RNA (including small RNAs) from a wide range of samples. You can opt to isolate small and large RNAs in
Midi Plant Genomic DNA Purification Kit
Midi Plant Genomic DNA Purification Kit Cat #:DP022MD/ DP022MD-50 Size:10/50 reactions Store at RT For research use only 1 Description: The Midi Plant Genomic DNA Purification Kit provides a rapid, simple
Grade 6 Standard 5 Unit Test Microorganisms. 1. Which of the following correctly describes the size of fungi compared to the size of bacteria?
Grade 6 Standard 5 Unit Test Microorganisms 1. Which of the following correctly describes the size of fungi compared to the size of bacteria? A. Fungi are larger. B. Bacteria are larger. C. They are about
Product Contents. 1 Specifications 1 Product Description. 2 Buffer Preparation... 3 Protocol. 3 Ordering Information 4
INSTRUCTION MANUAL Quick-RNA Midiprep Kit Catalog No. R1056 Highlights 10 minute method for isolating RNA (up to 1 mg) from a wide range of cell types and tissue samples. Clean-Spin column technology allows
ADVL0411 study. (temozolomide in patients with leukemia)
ADVL0411 study (temozolomide in patients with leukemia) I. Overview of study: We are interested in determining the level of MGMT activity and MRS mutations in leukemic samples (plasma and lymphoblasts)
Biol110L-Cell Biology Lab Spring Quarter 2012 Module 1-4 Friday April 13, 2012 (Start promptly; work fast; the protocols take ~4 h)
Biol110L-Cell Biology Lab Spring Quarter 2012 Module 1-4 Friday April 13, 2012 (Start promptly; work fast; the protocols take ~4 h) A. Microscopic Examination of the Plasma Membrane and Its Properties
Roadmap. Inbreeding How inbred is a population? What are the consequences of inbreeding?
1 Roadmap Quantitative traits What kinds of variation can selection work on? How much will a population respond to selection? Heritability How can response be restored? Inbreeding How inbred is a population?
The Importance of Hand Hygiene. Training material developed in collaboration with:
The Importance of Hand Hygiene Training material developed in collaboration with: Objectives Identify infection risks for staff and patients Understand basic steps to hand hygiene using Centers for Disease
Student Manual. Background STUDENT MANUAL BACKGROUND. Enzymes
Background Enzymes Enzymes are typically proteins (some nucleic acids have also been found to be enzymes) that act as catalysts, speeding up chemical reactions that would take far too long to occur on
How Can You Stay Fit?
How Can You Stay Fit? Introduction: In this lesson, students will learn about the health benefits of aerobic exercise, as well the importance of other types of physical activity such as stretching. This
TECHNICAL GUIDANCE FOR THE ACCREDITATION OF ANDROLOGY LABORATORIES
TECHNICAL GUIDANCE FOR THE ACCREDITATION OF ANDROLOGY LABORATORIES Approved By: Chief Executive Officer: Ron Josias Accreditation Executive: Mpho Phaloane Revised By: Medical Specialist Technical Committee
1. How many parents are involved in sexual reproduction? 2. What are the male and female sex cells called?
Warm up (3 minutes) 1. Pick up a warm up 2. Pick up the student work from the front table 3. Pick up your binder in the filing cabinet 4. Complete the warm up 5. Secure all handouts in your binder 1. How
RSID-Rapid Stain Test
RSID-Rapid Stain Test Prices valid in Germany 10. April 2015 RSID SINGLE BUFER SYSTEM Laboratory Kits: 0130 RSID SALIVA single buffer 25 Tests/Kit 0230 RSID SEMEN single buffer 25 Tests/Kit 0330 RSID BLOOD
Product Contents. 1 Specifications 1 Product Description. 2 Buffer Preparation... 3 Protocol. 3 Ordering Information 4 Related Products..
INSTRUCTION MANUAL Quick-RNA MidiPrep Catalog No. R1056 Highlights 10 minute method for isolating RNA (up to 1 mg) from a wide range of cell types and tissue samples. Clean-Spin column technology allows
Rescue IVF protocol for legacy stock
Rescue IVF protocol for legacy stock Sperm thawing/ivf protocol for MTG sperm samples (80ul per straw) from straw and conventional CPA from Vial (100ml per vial) This protocol is based on methods developed
TROUBLESHOOTING PGT CRB Workshop. PhD, HCLD, CC
TROUBLESHOOTING PGT 2018 CRB Workshop Jean M. Popwell PhD, HCLD, CC PREIMPLANTATION GENETIC TESTING (PGT) WHAT IS TROUBLESHOOTING? Planning ahead is always a great goal but we all should learn how to TROUBLESHOOT
Name: Enzymes. Class: Date: 101 minutes. Time: 100 marks. Marks: Level 1,2 and 3. Increasing demand. Comments:
Enzymes Name: Class: Date: Time: 0 minutes Marks: 00 marks Comments: Level,2 and 3. Increasing demand Q. Catalase is an enzyme. Catalase controls the following reaction: hydrogen peroxide water + oxygen
They know how to prevent colon cancer
They know how to prevent colon cancer and you can, too. Take a look inside. If you re 50 or older, you need to get tested for colon cancer. It s one cancer that can actually be prevented! Colon cancer:
Name: There are two things that will determine which particles will pass through and which will not:
18 Diffusion and Osmosis in Living Systems Name: Problem: How do substances move into and out of cells? Introduction: In order for cells to carry on their life processes, they must take in materials and
How Amylase Works. Copyright 2008 LessonSnips
How Amylase Works We chew our food to make the pieces smaller and saliva is added to make it softer and easier to swallow. Smaller softer pieces are easier to digest. To digest means to break down food
Pinpoint Slide RNA Isolation System II Catalog No. R1007
INSTRUCTION MANUAL Pinpoint Slide RNA Isolation System II Catalog No. R1007 Highlights Allows for the isolation of total RNA from paraffin-embedded tissue sections on glass slides Simple procedure combines
How would you prepare 455 grams of an aqueous solution that is 6.50% sodium sulfate by mass?
62 How would you prepare 455 grams of an aqueous solution that is 6.50% sodium sulfate by mass? Start a concentration calculation by writing the definition of the unit(s) you're using! We know everything
INFRAFRONTIER-I3 - Cryopreservation training course. Hosted by the Frozen Embryo and Sperm Archive, MRC - Harwell
Hosted by the Frozen Embryo and Sperm Archive, MRC - Harwell IVF recovery procedure incorporting methyl-β-cyclodextrin and reduced glutathione This protocol is based on the work published by Takeo et al.,
Welcome to the webinar. The presentation will begin shortly.
Welcome to the webinar. The presentation will begin shortly. You may listen via phone or via computer audio if your computer has speakers. This webinar will be in a listen only mode with opportunity to
Chromatin Immunoprecipitation (ChIPs) Protocol (Mirmira Lab)
Chromatin Immunoprecipitation (ChIPs) Protocol (Mirmira Lab) Updated 12/3/02 Reagents: ChIP sonication Buffer (1% Triton X-100, 0.1% Deoxycholate, 50 mm Tris 8.1, 150 mm NaCl, 5 mm EDTA): 10 ml 10 % Triton
Thawing MEFs (Mouse Embryonic Fibroblasts (MEFs)
1 FEEDER CULTURES The function of feeder cultures is to support the undifferentiated growth of hpsc. Typically primary fibroblasts are used for this purpose. We prepare our mouse feeder cells from ICR
What makes up our blood?
Serology Ch. 12 What makes up our blood? RED BLOOD CELLS (Erythrocytes) The most abundant cells in our blood; they are produced in the bone marrow and contain a protein called hemoglobin that carries oxygen
Having a vasectomy Urology department
Having a vasectomy Urology department 01935 384 345 yeovilhospital.nhs.uk Vasectomy is the permanent method of contraception for men. It is safe, simple and effective. What is vasectomy? Vasectomy is a
Blood Basics What makes up our blood? tissue infection wounds Karl Landsteiner
Blood Basics What makes up our blood? The most abundant cells in our blood; they are produced in the bone marrow and contain a protein called hemoglobin that carries oxygen to our cells. They are part
Collection of Dried Blood Spots from Infants for Diagnosis of HIV by DNA PCR. MOH Regional Trainings March 2013
Collection of Dried Blood Spots from Infants for Diagnosis of HIV by DNA MOH Regional Trainings March 2013 Outline Introduce DBS testing Rationale Testing algorithm Sample collection, storage and transportation
Single Cell Quantitative Polymer Chain Reaction (sc-qpcr)
Single Cell Quantitative Polymer Chain Reaction (sc-qpcr) Analyzing gene expression profiles from a bulk population of cells provides an average profile which may obscure important biological differences
ORAL HYGIENE SESSION 2
ORAL HYGIENE SESSION 2 BSCS_flipchart_session2.indd 1 dental FLOSS Floss is a special string used to clean between your teeth. This is a very hard space to get clean Floss can get to small spaces your
Human Alpha 1 microglobulin ELISA Kit
Human Alpha 1 microglobulin ELISA Kit Catalogue No.: EH4144 Size: 48T/96T Reactivity: Human Range:0.625-40ng/ml Sensitivity:
PROTOCOL: OPTIMIZATION OF LENTIVIRAL TRANSDUCTION USING SPINFECTION
Last Modified: April 2018 Last Review: October 2018 PROTOCOL: OPTIMIZATION OF LENTIVIRAL TRANSDUCTION USING SPINFECTION Table of Contents 1. Brief Description 1 2. Materials and Reagents.1 3. Optimization
Onion Root Tip Lab Report
Onion Root Tip Lab Report Hannah Scott Biology Ms. Carpenter February 25, 2013 Problem, Hypothesis and Prediction In this lab experiment, Hannah was to analyze an onion root tip under a microscope to observe
Methodology for the Extraction of Brain Tissue Protein. Learning Objectives:
Proteomics Extraction of Brain Tissue Protein Methodology for the Extraction of Brain Tissue Protein Extraction of the entire protein from the sample requires optimized protocol and many protocols have
Name Date Period. Macromolecule Virtual Lab. Name: Go to the website:
Macromolecule Virtual Lab Name: Go to the website: http://faculty.kirkwood.edu/apeterk/learningobjects/biologylabs.htm The most common organic compounds found in living organisms are lipids, carbohydrates,
A Guide to. Colon Cancer. Screening. Why should I get screened? a not too old adventure. ...because
A Guide to Colon Cancer Screening Why should I get screened? I have a all depend they lot on of cooking to do. me. I have a girls only my lothave of cooking to do. one mom. I have a practice we lot what
Physical activity and heart and circulatory diseases
Physical activity and heart and circulatory diseases UNDERSTANDING PHYSICAL ACTIVITY Heart and circulatory diseases kill 1 in 4 people in the UK. Not being active enough is one of the reasons people get
1 (b) (i) ACCEPT how long it took. time / time taken ; PhysicsAndMathsTutor.com
Question Answer Mark Guidance 1 (a) (works) outside cells ; 1 ACCEPT secreted / AW, from cells ACCEPT works in named extracellular environment e.g. digestive tract IGNORE doesn t work in cells 1 (b) (i)
Terminology-Amino Acids
Enzymes 1 2 Terminology-Amino Acids Primary Structure: is a polypeptide (large number of aminoacid residues bonded together in a chain) chain of amino acids linked with peptide bonds. Secondary Structure-
The author(s) shown below used Federal funds provided by the U.S. Department of Justice and prepared the following final report:
The author(s) shown below used Federal funds provided by the U.S. Department of Justice and prepared the following final report: Document Title: Author(s): DNA Profiling of the Semen Donor in Extended
REPRODUCTION: THE CONTINUITY OF LIFE
Suggested time allotment: 5 to 6 hours MODULE 4 REPRODUCTION: THE CONTINUITY OF LIFE Overview The beginning of a new life is truly a remarkable event. The sight of a chick making its way out of the cracked
AP BIOLOGY. Investigation #7 Cell Division: Mitosis and Meiosis. Slide 1 / 35. Slide 2 / 35. Slide 3 / 35. Investigation #1: Artificial Selection
New Jersey Center for Teaching and Learning Slide 1 / 35 Progressive Science Initiative This material is made freely available at www.njctl.org and is intended for the non-commercial use of students and
To provide you with necessary knowledge and skills to accurately perform 3 HIV rapid tests and to determine HIV status.
Module 9 Performing HIV Rapid Tests Purpose To provide you with necessary knowledge and skills to accurately perform 3 HIV rapid tests and to determine HIV status. Pre-requisite Modules Module 3: Overview
Procedure for Blood Analysis. 1.0 Purpose - This procedure specifies the methods for performing blood analysis in forensic casework.
Procedure for Blood Analysis 1.0 Purpose - This procedure specifies the methods for performing blood analysis in forensic casework. 2.0 Scope - This procedure applies to those Forensic Scientists who have
Instructions for Use. APO-AB Annexin V-Biotin Apoptosis Detection Kit 100 tests
3URGXFW,QIRUPDWLRQ Sigma TACS Annexin V Apoptosis Detection Kits Instructions for Use APO-AB Annexin V-Biotin Apoptosis Detection Kit 100 tests For Research Use Only. Not for use in diagnostic procedures.
Living Well with Diabetes. Meeting 12. Welcome!
12-1 Welcome! Welcome back and congratulations! Today is a time to celebrate all of your accomplishments. For the past few months we have learned a great deal about managing diabetes. Today, we will talk
Same Day, Cost-Effective Aneuploidy Detection with Agilent Oligonucleotide array CGH and MDA Single Cell Amplification Method
Same Day, Cost-Effective Aneuploidy Detection with Agilent Oligonucleotide array CGH and MDA Single Cell Amplification Method Presenter: Dr. Ali Hellani, Founder, Viafet Genomic Center, Dubai Wednesday,
Lipid (Oil Red O) staining Kit
Lipid (Oil Red O) staining Kit Catalog Number KA4541 1 Kit Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
Rotavirus Test Kit. Instructions For Use. Format: Cassette Specimen: Fecal Extract Catalog Number: VEL-001-ROTA
Rotavirus Test Kit Instructions For Use Format: Cassette Specimen: Fecal Extract Catalog Number: VEL-001-ROTA * Please read the instructions carefully before use INTENDED USE Velotest Rotavirus Test is
ExoQuick PLUS Exosome Purification Kit for Serum & Plasma
ExoQuick PLUS Exosome Purification Kit for Serum & Plasma Cat# EQPL10A-1 User Manual Store kit at +4 0 C Version 2 2/22/2017 A limited-use label license covers this product. By use of this product, you
DNA Profiling of the Semen Donor in Extended Interval (72 h) Post Coital Cervicovaginal Samples
DNA Profiling of the Semen Donor in Extended Interval (72 h) Post Coital Cervicovaginal Samples Jack Ballantyne University of Central Florida, Orlando, FL National Center for Forensic Science Problem some
year 7 REVISION Spring Assessment - Biology
year 7 REVISION Spring Assessment - Biology year 7 REVISION Microscope and Cells THE MICROSCOPE Used to view things too small for the naked eye. Consists of lenses of different magnification to make things
MOATT Card-Style Version: A single page MOATT (PDF) is also available at the MASCC website.
MOATT V1.2 9/9/16 MOATT MASCC Teaching Tool for Patients Receiving Oral Agents for Cancer This teaching tool has been prepared to assist healthcare providers in the assessment and education of patients
ASSET Student Laboratory
ASSET Student Laboratory The Effect of Cigarette Smoke and Alcohol on Tetrahymena Background Information While the general effects of smoking and alcohol use on humans are well documented, it is useful
Investigation of sickle cell disease
Investigation of sickle cell disease Sickle cell disease Sickle cell disease is an inherited disorder which affects people from Africa, India, Middle East and the Mediterranean region The main forms of
Title: Column Chromatography of Green Fluorescent Protein
Title: Column Chromatography of Green Fluorescent Protein Approvals: Preparer Date_07Oct06 Reviewer: Mary Jane Kurtz Date 09Jul13 Part I Crude Isolation of GFP from Lysed Cells q Page 1 of 6 1. Purpose:
TRACP & ALP double-stain Kit
Table of Content I. Description... 2 II. Introduction... 2 III. Principles... 2 IV. Kit components... 3 V. Storage... 3 VI. Preparation of reagents... 3 VII. Methods... 4-7 Cell fixation... 4 Activity
Medical Forensic Response in Minnesota How Are We Doing? Frequently Asked Questions About Sexual Assault Examinations in Minnesota
Medical Forensic Response in Minnesota How Are We Doing? Frequently Asked Questions About Sexual Assault Examinations in Minnesota 1) Why did MNCASA conduct a survey of the medical forensic response in
Subject: Science Scheme of Work: Y7 7A Cells, Tissues, Organs and Systems. Term: Autumn. 7A - Cells, Tissues, Organs and Systems.
Subject: Science Scheme of Work: Y7 7A Cells, Tissues, Organs and Systems Term: Autumn Topic / Unit(s) Overview / Context Assessment/Mastery Success criteria Curriculum Opportunities 7A - Cells, Tissues,
CoQ10(Coenzyme Q10) ELISA Kit
CoQ10(Coenzyme Q10) ELISA Kit Catalogue No.: EU0196 Size: 48T/96T Reactivity: Universal Detection Range: 0.781-50ng/ml Sensitivity:
2018 Oocyte Recovery and ICSI
2018 Oocyte Recovery and ICSI Equine Medical Services is one of the world s leading producers of in vitro produced embryos. While standard IVF techniques are ineffective with horses, ICSI (Intracytoplasmic
Immobilization of Samples on Slide 1.0 Hoskins Lab Last modified 03/28/2017 Chris DeCiantis
Immobilization of Samples on Slide 1. For a PEGylated slide, use 1X PBS sterile filtered buffer solution. 2. Wash the mpeg/peg-biotin using 1X PBS. Flow on 20 μl of the PBS using a 20 μl tip. The solution
Product Data For Research Use Only. Not For Use In Diagnostic Procedures
Product Data For Research Use Only. Not For Use In Diagnostic Procedures Cultrex Cell Staining Kit Related product available from Trevigen. Catalog# Description Size Cultrex In Vitro Angiogenesis Assay
How to Stay Leaner By Skipping Breakfast
How to Stay Leaner By Skipping Breakfast Jason Maxwell, CPT 2 Page How to Stay Leaner By Skipping Breakfast Copyright 2014 by Jason Maxwell All rights reserved. No part of this work may be reproduced or
AF HDL and LDL/VLDL Assay Kit
A HDL and LDL/VLDL Assay Kit Catalog Number KA1656 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3
Name: Period: Date: Testing for Biological Macromolecules Lab
Testing for Biological Macromolecules Lab Introduction: All living organisms are composed of various types of organic molecules, such as carbohydrates, starches, proteins, lipids and nucleic acids. These
Lab 2: Investigating Variation Across Spatial Scales
Lab 2: Investigating Variation Across Spatial Scales What are scales and variation in a biological context? The world around us displays incredible diversity across many scales. Today s lab investigates
The scientific discovery that changed our perception of anxiety
The scientific discovery that changed our perception of anxiety For many years I have suffered with a very severe form of OCD (Obsessive Compulsive Disorder), anxiety, and clinical depression. I had no
Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit
Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit Catalogue No.: EM0002 Size: 96T Reactivity: Mouse Application: This immunoassay kit allows for the qualitative determination of HBsAg in Mouse serum
E.Z.N.A. SQ Blood DNA Kit II. Table of Contents
E.Z.N.A. SQ Blood DNA Kit II Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Blood Storage and DNA Yield...4 Preparing Reagents...5 100-500 μl Whole Blood Protocol...6
BIOSTATISTICAL METHODS
BIOSTATISTICAL METHODS FOR TRANSLATIONAL & CLINICAL RESEARCH Introduction #2: DIAGNOSTIC MEDICINE PROSTATE The prostate is part of a man s reproductive system. It is a gland surrounding the neck of the
Name Date. In this lab investigation you will investigate the movement of water through a selectively permeable membrane.
This lab will be hand-written in your data book AP Osmosis Labs Part A (was done in previous a previous class: Dialysis tube + Starch + Glucose) Part B: Osmosis Unknowns In this lab investigation you will
Lab 6: Cellular Respiration
Lab 6: Cellular Respiration Metabolism is the sum of all chemical reactions in a living organism. These reactions can be catabolic or anabolic. Anabolic reactions use up energy to actually build complex
Exploring Mitosis Lab IMPORTANT LEARNING OBJECTIVES INTRODUCTION cell cycle interphase division (mitosis)
Exploring Mitosis Lab Today s lab will give you a chance to get an up-close view of mitosis, the process by which eukaryotic cells divide to generate identical daughter cells. We re using garlic (A. sativium)
Body Fluid ID by Mass Spectrometry
Body Fluid ID by Mass Spectrometry Kevin Legg, PhD & Phillip Danielson, PhD This research was supported in part by DNA R&D Grants 2006-DN-BX-K001, 2009-DN-BX-K165, 2011-CD-BX-0205, and 2012-DN-BX-K035
December th Quarter Newsletter. Harbor Pointer
Harbor Pointe Condominium December 2018 4 th Quarter Newsletter Harbor Pointer [Edition 1, Volume 1] GRAND OPENING OF THE NEWLY BUILT MARINA Editor: LCAM -Horvath Plan to attend our official Grand Opening
Lab: Organic Compounds
Lab: Organic Compounds Name(s) Date Period Benchmark: SC.912.L.18.1: Describe the basic molecular structures and primary functions of the four major categories of biological macromolecules. Background:
CytoSelect 48-Well Cell Adhesion Assay (Fibronectin-Coated, Colorimetric Format)
Product Manual CytoSelect 48-Well Cell Adhesion Assay (Fibronectin-Coated, Colorimetric Format) Catalog Number CBA-050 48 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction
24.3. Lesson 24.3 Amino Acids and Their Polymers. Overview. In this lesson, you will cover the topics of amino acids, peptides, proteins and enzymes.
24.3 Lesson 24.3 Amino Acids and Their Polymers Objectives 24.3.1 Diagram the general structure of an amino acid. 24.3.2 Identify what determines the differences in the chemical and physiological properties
Experimental Procedure
1 of 7 9/12/2018, 2:19 PM https://www.sciencebuddies.org/science-fair-projects/project-ideas/humbio_p043/human-biology-health/iron-rich-foods (http://www.sciencebuddies.org/science-fair-projects /project-ideas/humbio_p043/human-biology-health/iron-rich-foods)
Protect the quality and safety of your food
Protect the quality and safety of your food Description of tool This tool provides useful information about protecting the quality and safety of food. School teachers can use this information while teaching
Possible Precursors to One-on-One Violence
Reaction to Mariah Burton Nelson* Presentation at 1995 WSPECW Asilomar, California Jackie As I understand my assignment, I am to react to Mariah from the perspective of the chapter** I wrote in Sharon's
METABOLISM OF d-mannohepttjlose. EXCRETION OF THE SUGAR AFTER EATING AVOCADO
METABOLISM OF d-mannohepttjlose. EXCRETION OF THE SUGAR AFTER EATING AVOCADO BY N. R. BLATHERWICK, HARDY W. LARSON, AND SUSAN D. SAWYER (From the Biochemical Laboratory of the Metropolitan Life Insurance
SAMPLE LITERATURE Please refer to included weblink for correct version. Morphology of Cancer Cells. Edvo-Kit #990. Experiment Objective:
Edvo-Kit #990 Morphology of Cancer Cells Experiment Objective: 990 In this lab, students will explore the morphology of normal and cancerous cells by staining pre-fi xed cells with methylene blue and eosin.
2. Identify and distinguish between homogeneous and heterogeneous mixtures.
Food Explorations Lab II: Examining Emulsions STUDENT LAB INVESTIGATIONS Name: Lab Overview In this investigation, you will add substances to oil and water to determine which one(s) will act as an emulsifier