Osmosis, Plasmolysis and Turgidity

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1 Osmosis, Plasmolysis and Turgidity Question Paper 2 Level Subject Exam Board Topic Sub-Topic Booklet GCSE Biology CCEA Osmosis and plant transport Osmosis, plasmolysis and turgidity Question Paper Time Allowed: Score: Percentage: 53 minutes /44 /100

2 Higher Tier 1

3 1 A group of students used a weight potometer to investigate the water loss of three different plants after 5 days. The table shows the results. Plant Loss in mass after 5 days /g Average rate of water loss /g per day A 8.0 B C Adapted from: (a) Complete the table by calculating the average rate of water loss for plant A. Show your working. (b) Suggest two environmental factors the students should have controlled during this investigation. 1. [1] 2. [1] 2

4 The students then counted the number of stomata found on the leaves of each plant. They calculated the average number of stomata per mm 2. The table shows the results. Plant Average number of stomata per mm 2 of leaf surface A 51 B 74 C 18 (c) Use data from both tables to describe and explain the results of the investigation. [4] 3

5 2 Osmosis has a different effect on animal and plant cells. (a) Explain what happens to a red blood cell when it is placed in water. [4] (b) Describe and explain why osmosis has a different effect on plant cells when placed in water. [3] The diagrams show an experiment carried out over three hours. weak sugar solution strong sugar solution potato with hollow centre water in dish Start 3 hours later 4

6 (c) Describe and explain the changes that occurred during the three hours. Suggest why similar changes would not occur using a boiled potato. In this question you will be assessed on your written communication skills, including the use of specialist scientific terms. [6] 5

7 3 A pupil set up an experiment using pond weed. Each tube contained the same volume of indicator solution. The tubes were left in different light conditions for 24 hours. Light condition at start Colour of indicator after 24 hours bright light pond weed indicator solution dim light red purple red dark red yellow Illustrated Biology Questions (1986), ISBN , Philip Booth and Geoffrey Hall, publisher: Pearson. Duplication is prohibited other than for teaching and study. (a) Describe the contents of a control tube for this experiment. [1 6

8 (b) It may not be valid to compare the results for each tube. Suggest one reason why. [1] (c) Give two other variables which must be controlled (d) Name the indicator solution used in this experiment. [1] (e) Explain the colour change observed in the test tube placed in bright light. [3] (f) The tube in dim light represents the compensation point. (i) Give the colour of the indicator in the tube in dim light after 24 hours. [1] (ii) What is meant by the compensation point? 7

9 4 The table shows the results of an experiment carried out to investigate the effect of placing potato cylinders into a range of sugar solutions kept at the same temperature for 24 hours. All the potato cylinders were taken from the same potato. Concentration of sugar solution/m Initial mass of potato cylinder/g Final mass of potato cylinder/g Change in mass of potato cylinder/g Percentage change in mass of potato cylinder (a) Use the information given to suggest two ways the experiment was a fair test. (b) Complete the table by calculating the percentage change in mass of the potato cylinder placed in 0.2 M solution. Show your working. 8

10 (c) Explain the percentage change in mass of the potato cylinder placed in 0.6 M solution. [3] (d) Draw a line graph of these results on the grid provided. 25 Percentage change in mass of potato cylinder Concentration of sugar solution/m 20 [3] 9

11 (e) Use the graph to find the concentration of sugar solution which is equal to the concentration of the cytoplasm in potato cells. Explain your answer. Concentration M Explanation 10

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