Asian Journal of Ecotoxicology. Cristaria plicata Cu Zn. Cu Zn. 8d p<0.05. Cu. Cu Zn. Zn. X52

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1 Asian Journal of Ecotoxicology Vol 5, 2010 No 2, Cu Zn * Cristaria plicata Cu Zn Cu Zn 8d p<005 Cu Zn p<005 Cu Zn Cu > > > p< Zn > > > p< Cu Zn Cu Zn Cu Zn Zn BCF Cu X52 A Accumulation and Distribution of Copper and Zinc in the Freshwater Mussel, Cristaria Plicata, Exposed to Individual and Mixed Metals XIA Tian-xiang 1 2 LIU Xue-hua 1 * ZHAO Meng-bin 2 1 Department of Environmental Science and Engineering, Tsinghua University, Beijing Beijing Glorious Land Agricultural Co, LTD Enterprise Postdoctoral Workstation of Zhongguancun Haidian Science Park, Beijing Received 8 January 2009 accepted 2 March 2009 Abstract Characteristics of accumulation and distribution of Cu and Zn in the freshwater mussel, Cristaria plicata, exposed to individual and mixed metals were investigated under the laboratory conditions Results showed that the Cristaria plicata mussel could concentrate Cu and Zn in each soft tissue, the contents in which linearly increased with exposure times from 0 to 8 days The tissues of gill and mantle accumulated Cu and Zn more rapidly than the other tissues p < 005 Significant differences in accumulation rates between Cu and Zn were found at the tissues of gill, viscera and gonad p <005 and the interaction of the two metals during accumulation was antagonistic Before the metal exposure, metal contents in different tissues were with the sequences of gonad >mantle, viscera >gill, foot >muscle and the contents varied 10 -fold range for Cu p<005, and gill>viscera >gonad>foot, mantle >muscle and the contents varied 4 -fold range for Zn p <005 The metal exposures caused Cu and Zn contents reached the highest values in the mantle and gill respectively and the differences in Cu contents and Zn contents among each tissue were improved The ranges of Cu contents varied in different tissues were higher than that of Zn contents varied before and after the exposure At the different metal exposures, the bioconcentration factor BCF of Zn is higher than that of Cu obviously in each tissue of the test mussel Keywords Cristaria plicata accumulation rate tissue copper zinc bioconcentration factor BCF No No K * Corresponding author E -mail: xuehua - hjx@mailtsinghuaeducn

2 Introduction Cu Zn Lecoeur et al, 2004 Boening, 2 Materials and methods Wang and Rainbow, d Dreissena polymorpha Roditi and Fisher, 1999 Hyriopsis cumingii 2002 Corbicula fluminea Cd Ag Hg Pb 22 Cristaria plicata CuSO 4 5H 2 O ZnSO 4 7H 2 O Cu Zn 23 8L Harrison and Hoare, 5L 1980 Loayza -Muro Elías -Letts 2007 Herkovits and Helguero, Cu Ojo et al, μg L -1 Zn 2000μg L -1 Cu Zn Cu Zn Cu Zn Zn Cu 10 Cu Herkovits and Helguero, μg L -1 Zn 2000μg L -1 Cu +Zn 2000μg L μg L -1 CK CK 12 Philips, 1976; Ahsannullah et al, 1981; Elliott et al, d 3 8d Yap et al, 2003; Gundacker, 1999; 1 400~500g 16cm 4d GB d Kádár et al, 2006 Widdows Cu Zn 2

3 2 Cu Zn ; Clason et al, 2003; 2004; Ruelas -Inzunza and Páez -Osuna, ~ 02g 5mL 70% 25mL PE AA -300 USA 1999 Liu and Deng, 2007 Cu Zn Cu Zn 937% 955% 25 R 2 067~097 EXCEL SPSS 130 Zn R 2 046~097 Zn Cu Yap et al, 2003 Zn t BCF Bioconcentration factor 1 BCF = C e C s Cu Zn t C e mg kg -1 Cu C s mg L -1 Zn 3 Results and analysis 31 Cu Zn K 1 Cu Cu Zn p < p<005 K 1 -K 2 K 3 -K 4 Cu Zn K 1 -K 3 K 2 -K 4 Cu Zn Cu Zn Cu Zn 1 2 MT Kǎgi and Kojima, 1987 MT 2004 Lecoeur et al, 2004 Table 1 1 Cu Zn K mg kg -1 d -1 Bioaccumulation rates K mg kg -1 d -1 of Cu and Zn in each tissue of Cristaria plicata exposed to individual and mixed metals a Cu Cu Zn Zn Cu+Zn Cu Cu+Zn Zn K K 1 R 2 K 2 R 2 K 3 R 2 K 4 R 2 K 1 - K 3 K 2 - K 4 K 1 - K 2 K 3 - K 4 22 d c c c * 288 b a a a ** 003 * 003 * 394 a b a b ** * 36 d c c c ** 001 * 18 d c c d c c b d * * 002 * K p>005 K p<005 a t * ** p<005 p<001

4 172 5

5 2 Cu Zn 173 Roesijadi, 1994 > > p<005 Cu Cu Zn Cu Zn K 1 -K 3 p <005 Zn Cu K 2 -K 4 p<001 Zn p>005 Yap et al, 2003; Gundacker, 1999; Kádár et al, MT 2006 Yap et al, Cu Zn 300μg L -1 Cu 300μg L -1 Cu Zn Zn Cu 2 Cu > > > MT p<005 Zn > > Philips et al, 1993 Cu Zn Zn Table 2 2 Cu Zn mg kg -1 BCF Contents and bioconcentration factor BCF of Cu and Zn in each tissue of Cristaria Plicata before and after exposure to individual and mixed metals C i-cu C i-zn Cu Zn Cu + Zn Cu + Zn C e-cu BCF C e-zn BCF C e-cu BCF C e-zn BCF 85 c 1338 d 167 c c c c c 3020 a 1094 b a a a b 1320 d 1779 a b a b b 1657 b 271 c c c c d 732 e 87 c e c e a 1488 c 846 b c b c 8735 C i C e p>005 p<005 Zn Cu 7~ 30 Cu 10 Cu Zn 435~ ~24545 Zn 4 Cu Shi Wang Cu Zn 16 Zn Perna viridis 6 5 Cu Zn BCF Cu Cu Zn Zn Yap et al, 2003; Philips, 1985; Rees et al, 1999 Yap et Cu Zn, Zn Cu Zn Cu Cd Cu Zn al, 2003 Cu Zn BCF McGeer et al, 2003 Cu Zn Zn Cu

6 174 5 Liu W, Deng P Y 2007 Accumulation of cadmium, copper, lead and zinc in the Pacific Oyster, Crassostrea gigas, collected from the Pearl River Estuary, Southern China J Bulletin of 1964 Environmental Contamination and Toxicology, 78 6 : Loayza -Muro R, Elías -Letts R 2007 Responses of the mussel Anodontites trapesialis Unionidae to environmental stressors: effects of ph, temperature and metals on filtration rate J Environmental Pollution, : References Ahsannullah M, Negilski D S, Mobley M C 1980 Toxicity of zinc, cadmium and copper to the shrimp Callianassa australiensis Ш Accumulation of metals J Marine Biology, 64 3 : Boening D W 1999 An evaluation of bivalves as biomonitors of heavy metals pollution in marine waters J Environmental Monitoring and Assessment, 55 3 : Cai L Z, Hong H S, Hong L Y 1999 Accumulation of zinc and lead in Ruditaps philippinarun and its tolerance J Acta Scientiae Circumstantiae, 19 3 : in Chinese Chen H G, Jia X P, Lin Q, Ma S W, Cai W G, Wang Z H 2008 Accumulation and release characteristics of heavy metals in Crassostrea rivalaris under mixed exposure J Chinese Journal of Applied Ecology, 19 4 : in Chinese Clason B, Duquesne S, Liess M, Schulz R, Zauke G P 2003 Bioaccumulation of trace metals in the Antarctic amphipod Paramoera walkeri Stebbing, 1906 : Comparison of two - compartment and hyperbolic toxicokinetic models J Aquatic Toxicology, 65 2 : Clason B, Gulliksen B, Zauke G P Assessment of two - compartment models as predictive tools for the bioaccumulation of trace metals in the amphipod Gammarus oceanicus Segerstrale, 1947 from Grunnfjord Northern Norway J Science of the Total Environment, : Elliott N G, Swain R, Ritz D A 1986 Metal interaction during accumulation by the mussel Mytilus edulis planulatus J Marine Biology, 93 3 : Gundacker C 1999 Tissue -specific heavy metals Cd, Pb, Cu, Zn deposition in a natural population of the zebra mussel Dreissena polymorpha Pallas J Chemosphere, : Harrison P M, Hoare R J 1980 Metals in Biochemistry M London: Chapman & Hall Herkovits J, Helguero L A 1998 Copper toxicity and copper - zinc interactions in amphibian embryos J Science of the Total Environment, : 1-10 Kádár E, Costa V, Santos R S 2006 Distribution of micro - essential Fe, Cu, Zn and toxic Hg metals in tissues of two nutritionally distinct hydrothermal shrimps J Science of the Total Environment, 358: Kǎgi J H, Kojima Y 1987 Chemistry and biochemistry of metallothionein J Experientia Supplementum, 52: Lecoeur S, Videmann B, Berny P 2004 Evaluation of metallothionein as a biomarker of single and combined Cd/Cu exposure in Dreissena polymorpha J Environmental Research, 94 2 : McGeer J C, Brix K V, Skeaf J M, DeForest D K, Brigham S I, Adams W J, Green A 2003 Inverse relationship between bioconcentration factor and exposure concentration for metals: Implications for hazard assessment of metals in the aquatic environment J Environmental Toxicology and Chemistry, 22 5 : Ministry of Environmental Protection of the People s Republic of China 2002 GB Environmental quality standards for surface water S Beijing: China Environmental Science Press in Chinese Ojo A A, Nadella S R, Wood C M 2008 In vitro examination of interactions between copper and zinc uptake via the gastrointestinal tract of the Rainbow Trout Oncorhynchus mykiss J Archives of Environmental Contamination and Toxicology, 56 2 : Philips D J H 1976 The common mussel Mytilus edulis as an indicator of pollution by zinc, cadmium, lead and copper Effects of environmental variables on uptake of metals J Marine Biology, 38 1 : Philips D J H 1985 Organochlorines and trace metals in green lipped mussels Perna viridis from Hong Kong waters: a test of indicator ability J Marine Ecology Progress Series, 21: Phillips D J H, Rainbow P S 1993 Biomonitoring of Trace Aquatic Contaminants M London: Elsevier Science Publishers limited Rees J G, Setiapermana V A, Sharp T M, Weeks J M, Williams T M 1999 Evaluation of the impacts of land -based contaminants on the benthic faunas of Jakarta Bay, Indonesia J Oceanologica Acta, 22 6 : Roditi H A, Fisher N S 1999 Rates and routes of trace element uptake in zebra mussels J Limnology and Oceanography, 44 7 : Roesijadi G 1994 Behavior of metallothonein -bound metals in a natural population of an estuarine mollusca J Marine Environmental Research, 38 4 : Ruelas-Inzunza J R, Páez-Osuna F 2000 Comparative bioavailability of trace metals using three filter -feeder organisms in a subtropical coastal environment Southeast Gulf of California J Environmental Pollution, 107: Shi D, Wang W 2004 Modification of trace metal accumulation in the green mussel Perna viridis by exposure to Ag, Cu, and Zn J Environmental Pollution, 132: Wang W, Rainbow P S 2008 Comparative approaches to understand metal bioaccumulation in aquatic animals J Comparative Biochemistry and Physiology -Part C, :

7 2 Cu Zn 175 Widdows J, Donkin P 1992 Mussel and environmental contaminants: bioaccumulation and physiological aspects A // Gosling E The Mussel Mytilus: Ecology, Physiology, Genetics and Culture C Amsterdam: Elseviers Science Publishers, Xiang L X, Shao J Z 2002 Decontamination and absorption of water Cr, Pb and Cd pollution by mussel Hyriopsis cumin Lea J Journal of Zhejiang University Science Edition, 29 5 : in Chinese Yap C K, Ismail A, Tan S G, Omar H 2003 Accumulation, depuration and distribution of cadmium and zinc in the green - lipped mussel Perna viridis Linnaeus under laboratory conditions J Hydrobiologia, : Zeng L X, Chen G Z, Yu R Q, Lu J 2004 Accumulation effects of biomonitoring indicator Asian Clam Corbicula fluminea under different Cd and Cu pollution conditions J Journal of Agro -Environment Science, 23 5 : in Chinese Zhang S N, Sun Y, Song Y L, Yu Z G 2004 Kinetic features of four heavy metals bioaccumulation of mussel Mytilus edulis J Oceanologia et Limnologia Sinica, 35 5 : in Chinese,, 1999 J, 19 3 : ,,,,, 2008 J, 19 4 : GB S :, 2002 Cr, Pb Cd J, 29 5 : ,,, 2004 Cd Cu J, 23 5 : ,,, 2004 Mytilus edulis 4 J, 35 5 :