QuEchERS Solid Phase Extraction

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1 QuEchERS Solid Phase Extraction Silica Based SPE Non-Silica based SPE Polymer-based SPE Abel Industries (Canada) Ltd. PO Box 31692, Meadowvale S C Pitt Meadows, BC, V3Y 2H1, Canada Tel: Fax: info@abel-industries.com Web:

Abel Industries QuEChERS QuEChERS a Simplified Method of Sample Preparation for Pesticide Analysis In 2003, M Anastassisdes developed a simple way to prepare food samples for pesticide analysis.

2 Abel Industries QuEChERS QuEChERS a Simplified Method of Sample Preparation for Pesticide Analysis In 2003, M Anastassisdes developed a simple way to prepare food samples for pesticide analysis. It s called QuEChERS (pronounced as Catchers ), an acronym for Quick, Easy, Cheap, Effective, Rugged and Safe. With QuEChERS, you could prepare your samples in multi-class, multi-residue pesticide analysis while saving time and money. Why ABEL QuEChERS? Saving time and money for your sample preparation High quality, best reproducibility Lowest contamination Lower cost as compared to other brands ABEL QuEChERS How QuEChERS works? QuEChERS extraction method is designed for multi-residue pesticide analysis of fruits and vegetables with high water content (80%-95%). Some compounds will interfere the detection of target pesticide, such as chlorophyll, fatty acid, pigment, which could be removed by QuEChERS. 1

Abel Industries QuEChERS 2003 Anastassiades,Lehotay,etal 2007 Lehotay 2008 Anastassisdes Original AOAC 2007.

mgso 4 ABEL QuEChERS Shake Centrifuge 150mg/mL MgS0 4 +25mg/mL PSA Shake and Centrifuge +1.5g anh.naoac Shake Centrifuge 150mg/mL MgS0 4 +50mg/mL PSA Shake and Centrifuge +1g Na3Cit.2H 2 O +0.

3 Abel Industries QuEChERS 2003 Anastassiades,Lehotay,etal 2007 Lehotay 2008 Anastassisdes Original AOAC CEN g Subsample 15g Subsample 10g Subsample 10mL ACN 15mL ACN with 10mL ACN 4g anh.mgso 4 1%HOAC Shake +1g NaCl 6g anh.mgso 4 4g anh.mgso 4 ABEL QuEChERS Shake Centrifuge 150mg/mL MgS mg/mL PSA Shake and Centrifuge +1.5g anh.naoac Shake Centrifuge 150mg/mL MgS mg/mL PSA Shake and Centrifuge +1g Na3Cit.2H 2 O +0.5g Na2Cit.5H 2 O Shake Centrifuge 150mg/mL MgS mg/mL PSA Shake and Centrifuge Some QuEChERS methods add buffers during the extraction step. Two most common methods are the European Committee for Standardization (CEN 15662) method which applies citrate buffer for extraction and the Association of Analytical Communities (AOAC ) method using acetic acid buffer. For low-water content fruits and vegetables, additional water needs to be added to optimize the extraction. For high-fatty content fruits and vegetables, C18E should be added to remove it. For high-pigment content fruits and vegetables, graphicarb should be added. 2

4 Abel Industries QuEChERS ABEL QuECHERS Workflow Step 1: Extraction Transfer homogenized sample (10g or 15g) to 50ml tube, then add extraction solvent and salt, shake for one minute, centrifuge > 1500rcf for 1 minute. ABEL QuEChERS Extraction Kits: 50ml tubes Pre-packaged MgSO 4, NaCl or other salts ABEL QuEChERS Extraction Bags Ordering Information (without 50ml tubes) ABEL QuEChERS Part Numbers Pack Size MgSO Na Acetate Na Citrate Na Citrate Sesquihtdrate NaCl AOAC QuEChERS Extraction Bags for 15 g samples EN QuEChERS Extraction Bags for 10 g Samples Original QuEChERS Extraction Bags 10 g Samples ABEL-QE g 1.5 g ABEL-QE g 1 g 0.5 g 1 g ABEL-QE-03 4 g 1 g Step 2: Clean up Choose right clean up tube, transfer the extracted sample in Step 1 to clean up tube, shake for 30 seconds, then centrifuge > 1500 rcf for 1 minute. 3

5 Abel Industries QuEChERS ABEL QuEChERS Clean-up Tubes Ordering Information Part Numbers Tubes Pack Size MgSO PSA C18E GCB AOAC Method fruits and vegetables EN Method fruits and vegetables ABEL-QC ml mg 50 mg ABEL-QC ml mg 400 mg ABEL-QC ml mg 25 mg ABEL-QC ml mg 150 mg ABEL QuEChERS AOAC Method Waxy or fatty fruits and vegetables EN Method Waxy or fatty fruits and vegetables AOAC Method Pigment content fruits and vegetables AOAC Method Pigment content fruits and vegetables ABEL-QC ml mg 50 mg 50 mg ABEL-QC ml mg 400 mg 400 mg ABEL-QC ml mg 25 mg 25 mg ABEL-QC ml mg 150 mg 150 mg ABEL-QC ml mg 50 mg 50 mg ABEL-QC ml mg 400 mg 400 mg ABEL-QC ml mg 25 mg 2.5 mg ABEL-QC ml mg 150 mg 15 mg AOAC Method High pigment and fatty content fruits and vegetables EN Method High pigment content fruits and vegetables ABEL-QC ml mg 50 mg 50 mg 50 mg ABEL-QC ml mg 400 mg 400 mg 400 mg ABEL-QC ml mg 25 mg 7.5 mg ABEL-QC ml mg 150 mg 45 mg What are these sorbents used for? MgSO Remove H 2 O in the matrix PSA Adsorb the carbohydrate, fatty acid, organic acid and some pigment in the matrix C18E Remove non-polar compounds in the matrix GCB Remove pigment, sterol in the matrix 4

Abel Industries QuEChERS Bulk Sorbents Sorbent Name Part Number Unit Packing Size PSA

Sorbent Name Part Number Specification Packing Size ABEL-S46PS-0302 200 mg/3 ml 50 PSA

ABEL QuEChERS C18E ABEL-S4618E-0303 200 mg/3 ml 50 ABEL-S4618E-0605 500 mg/6 ml 30 GCB

6 Abel Industries QuEChERS Bulk Sorbents Sorbent Name Part Number Unit Packing Size PSA ABEL-S46PS-0100 g 100 NH ABEL-S46NH-0100 g 100 C18E ABEL-S4618E-0100 g 100 GCB ABEL-G g 100 Sorbent Name Part Number Specification Packing Size ABEL-S46PS mg/3 ml 50 PSA ABEL-S46PS mg/6 ml 30 ABEL-S46NH mg/3 ml 50 NH ABEL-S46NH mg/6 ml 30 ABEL QuEChERS C18E ABEL-S4618E mg/3 ml 50 ABEL-S4618E mg/6 ml 30 GCB ABEL-G ABEL-G mg/3 ml 500 mg/6 ml Carb/NH ABEL-GN ABEL-G0N mg/250 mg, 3 ml 500 mg/500 mg, 6 ml

Abel Industries QuEChERS the Clean-up result from Spinage using different content of Abel GraphiCarb ABEL QuEChERS No Clean-Up PSA Only PSA + 5 mg GCB PSA + 10 mg GCB PSA + 20 mg GCB Application: the

7 Abel Industries QuEChERS the Clean-up result from Spinage using different content of Abel GraphiCarb ABEL QuEChERS No Clean-Up PSA Only PSA + 5 mg GCB PSA + 10 mg GCB PSA + 20 mg GCB Application: the analysis of multi-residue pesticide in apple using Abel QuEChERS Kit PSA + 50 mg GCB Extraction 1. Transfer homogenized sample (10g or 15g) to 50ml tube 2. Add 15ml CAN with 1% HOAC 3. Add internal standard 4. Shake for 1 min 5. Add ABEL-QE-02 extraction salt into tube 6. Shake for 1 min 7. Centrifuge > 4000rpm for 5 min 8. Get the above liquid Clean up 1. Add 1 ml above liquid sample into ABEL-QC-1102 clean up tube, shake for 1 min 2. Add 8 ml above liquid sample into ABEL-QC-1115 clean up tube, shake for 1 min 3. Centrifuge > 13000rpm for 2 min 4. Get the above liquid for injection 6

8 Abel Industries QuEChERS GC Method Autosampler: Agilent 7683 AS Column: Agilent J W HP-5ms GC Column,30 m*0.25 mm, 0.25um Gas: He Temp: 70 (2min),25 /min to 150 (0min),3 /min to 200 (0min),8 /min to 280 Volume: 1.0ul Mass Method Mode: SIM Source: Quadrupole Interface Temp: 230, 150 and 280 ABEL QuEChERS 7

Abel Industries QuEChERS Results 10 ng/g 50 ng/g 200 ng/g Target Recovery RSD(n=6) Recovery RSD(n=6) Recovery RSD(n=6) Dichlorvos 86.8 7.0 83.9 11.6 81.5 5.5 2-Phenyl phenol 113.4 6.3 96.3 6.5 100.

0 Dichloroacetophenone N-(trichloromethylthio)phthalimide Endosulfan Dieldrin 97.8 7.5 95.5 6.2 104.5 4.5 74.4 9.3 94.6 10.3 9.6 4.7 88.8 4.6 95.3 3.4 69.8 9.2 91.3 5.4 96.2 5.4 90.5 10.3 86.6 3.4 92.

9 Abel Industries QuEChERS Results 10 ng/g 50 ng/g 200 ng/g Target Recovery RSD(n=6) Recovery RSD(n=6) Recovery RSD(n=6) Dichlorvos Phenyl phenol Diazinon Chlorothalonil ABEL QuEChERS Carbaryl Dichlofluanid Dichloroacetophenone N-(trichloromethylthio)phthalimide Endosulfan Dieldrin DDE Ethion Endosulfan sulfate Endrin Ketone Standard Permethrin Coumaphos

10 Solid Phase Extraction (SPE) Products 9

11 Introduction of Abel SPE products 10

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18 Abel Industries Diol ordering information Abel Industries SCX ordering information 17

19 Abel Industries SAX Abel Industries SAX ordering information Abel Industries WCX Abel Industries WCX ordering information Abel Industries PRS(propylsulfonic acid) Abel Industries PRS ordering information 18

20 2. Abel Industries inorganic non-silica based SPE Abel Industries Florisil Abel Industries Florisil ordering information Abel Industries Florisil PR Abel Industries Florisil PR ordering information 19

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Phenomenex roq QuEChERS Kits. Overview. Spinach Facts

Phenomenex roq QuEChERS Kits. Overview. Spinach Facts Optimisation of LC/MS Analysis of Pesticide Residues in Food Products When Using QuEChERS Technique Phenomenex roq QuEChERS Kits Aman Sharma Phenomenex ANZ amans@phenomenex.com 1 2 Background Overview