Multilayer SPE Tubes for Pesticide Analysis

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1 Page 1 of 6 Return to Web Version Solid Phase Extraction Multilayer SPE Tubes for Pesticide Analysis The multi-residue surveillance of pesticides in agricultural products (fruits, vegetables, meat, shellfish, grains, and dairy products) is an ongoing project for regulatory agencies, contract laboratories, and industrial laboratories worldwide. Hundreds of thousands of samples are analyzed annually to meet a variety of purposes including regulatory enforcement and surveillance monitoring. A number of procedures have been published for the extraction and analyses of multiresidue pesticides from a variety of food matrices. Most of them involve an initial liquid-liquid extraction step, and/or solid-liquid extraction step using a water miscible solvent such as acetonitrile or acetone. Subsequent analysis is conducted via GC-MS, GC-fluuorescence, GC-ESD, and other detection techniques. Further cleanup using SPE technology prior to GC analysis is necessary to: Decrease background levels for trace level pesticide detection Reduce matrix-induced signal enhancement Reduce down time on the GC system The Supelclean ENVI-Carb -II/PSA SPE product line consists of multi-layer SPE cartridges that were developed for superior cleanup when conducting multi-residue pesticide analysis in agricultural products (fruits, vegetables, meat, shellfish, grains, and dairy products). The technology acts as a chemical filter in which each layer plays a specific role for removing key interferences when conducting pesticide analysis using GC.

2 Page 2 of 6 View a comprehensive technical reort describing how multilayer SPE works, including example applications/protocols and suggested readings/references. Suel Shere arbon SPE etention Mechanis : Reversed-phase and anion-exchange SPE tube packed entirely with spherical, non-friable particles. Improved flow characteristics and faster flow for gravity filtration. Reduced susceptibility to the formation of fines. Dual layer SPE tube contains both spherical carbon (upper layer) & spherical aminopropyl (NH2) phase (lower layer). SPE sorbents are separated by PE frit. Developed to offer superior cleanup when conducting multi-residue pesticide analysis from food (e.g. agricultural products, meats, etc.). Carbon has a strong affinity towards planar molecules, and can isolate/remove pigments (e.g., chlorophyll and carotinoids) and sterols commonly present in foods and natural products. Aminopropyl (NH 2 retains fatty acids, organic acids, and some polar pigments and sugars common in food matrices. View addtional information Supel Sphere Carbon/NH 2 50 mg (spherical carbon) / 50 mg (spherical NH 2 ) / 6 ml U

3 2 Page 3 of 6 Suelclean E arb PSA SPE etention Mechanis : Reversed-phase and anion-exchange Dual layer SPE tube that contains both Supelclean ENVI-Carb-II (upper layer) and PSA (lower layer) SPE sorbents (separated by PE frit) Developed to offer superior cleanup when conducting multi-residue pesticide analysis in food (e.g., fruits, vegetables, etc.) ENVI-Carb-II a graphitized non-porous carbon (10/140 mesh, surface area 10 m /g) that has a strong affinity towards planar molecules, and has been quality controlled specifically for the isolation/removal of pigments (e.g., chlorophyll and carotinoids) and sterols commonly present in fruits, vegetables, and other natural products Supelclean PSA is a polymerically bonded, ethylenediamine-n-propyl phase that contains both primary and secondary amines Supelclean PSA has a strong affinity and high capacity for fatty acids, organic acids, and some polar pigments and sugars Tested for superior cleanliness using GC-FID and GC-MS Supelclean ENVI-Carb-II/PSA SPE Tube 30 mg / 60 mg / 6 ml U 50 mg / 50 mg / 6 ml U 50 mg / 30 mg / 6 ml U Suelclean E arb SAPSA SPE etention Mechanis : Reversed-phase and anion-exchange Tri-layer SPE tube that contains Supelclean ENVI-Carb-II (upper layer), SAX (middle layer) and PSA (lower layer) SPE sorbents (separated by PE frit)

4 2 Page 4 of 6 Developed to offer superior cleanup when conducting multi-residue pesticide analysis in food (e.g., fruits, vegetables, etc.) ENVI-Carb-II is a graphitized non-porous carbon (10/140 mesh, surface area 10 m /g) that has a strong affinity towards planar molecules, and has been quality controlled specifically for the isolation/removal of pigments (e.g., chlorophyll and carotinoids) and sterols commonly present in fruits, vegetables and other natural products Supelclean PSA has a strong affinity and high capacity for fatty acids, organic acids, and some polar pigments and sugars Supelclean SAX offers additional ion-exchange capacity for removing matrix components that may induce ion-suppression or enhancement during GC analysis Supelclean ENVI-Carb-II/SAX/PSA SPE Tube 50 mg / 50 mg / 50 mg / 12 ml U Suelclean SAPSA SPE etention Mechanis : Normal-phase and anion-exchange Dual layer SPE tube that contains both Supelclean SAX (upper layer) and PSA (lower layer) SPE sorbents (separated by PE frit) Supelclean SAX is a quarternary amine, C l - counter-ion Supelclean PSA is a polymerically bonded, ethylenediamine-n-propyl phase that contains both primary and secondary amines Ideal for removing matrix components (fatty acids, organic acids, polar pigments, and some sugars) when conducting multi-residue pesticide analysis in foods In compliance with Luke and Luke II methods that use SPE to reduce matrix induced ion-suppression and enhancement when conducting GC analysis of pesticides in food

5 Page 5 of 6 Supelclean SAX/PSA SPE Tube 520 mg / 250 mg / 6 ml U 50 mg / 50 mg / 6 ml U Suelclean PSA SPE (PSA Structure) etention Mechanis : Normal-phase and anion-exchange Polymerically bonded, ethylenediamine-n-propyl phase that contains both primary and secondary amines A weak anion exchanger with a pka of 10.1 and 10.9 Similar to aminopropyl SPE phases (NH 2 ) in terms of selectivity, but has a much higher capacity due to presence of secondary amine ( meq/g) Strong affinity and high capacity for removing fatty acids, organic acids, and some polar pigments and sugars when conducting multi-residue pesticide analysis in foods Has been shown to significantly reduce matrix-enhancement effects encountered during the GC analysis of food products Bidendate nature of ligands allow for chelation Supelclean PSA SPE Tube 20 mg / 3mL U 50 mg / 6 ml U Bulk packing 10 g U

6 Page 6 of 6 Supelclean ENVI-Carb SPE Retention Mechanism Sample Matrix Compatibility