Recent Developments in Biobank Technologies
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1 1 Recent Developments in Biobank Technologies Kristian Hveem, MD, PhD Professor in Clinical Epidemiology, NTNU Director of HUNT Biobank and Biobank Norway/BBMRI.no Scientific Counselor, Danish National Biobank
2 2 To be presented Biobank Networks Evaluation of biobanks Biobank processes Some significant technological improvements Evaluation platforms for biobank technologies
3 3 Biobank networks 2008: ESFRI road map for biobanks 2009: BBMRI.eu 2009: Early establishment of national BBMRI-nodes 2011: BioSHaRE.eu 2013: BBMRI-LPC 2014: BBMRI-ERIC A comprehensive working program recently introduced First calls for Common Services, ELSI and IT 2014: National biobank infrastructures in place in a significant number of European countries Funding by the ministries, national research councils and private trusts ranging from mill per country
4 4 Evaluation of biobanks it is not all about high-tech, highthroughput, automated solutions
5 5 Biobank evaluation criteria (ESBB) Quality (materials and operations) Transparency (outreach activities and donor relations) Usage (sample turnover) Connectivity (integration into Biobank networks and trans-institutional infrastructure) Innovation (innovative solutions for any kind of Biobank-related problems) Sustainability (outline of business model)
6 6 Biobank processes it is all about quality.. quality, the driving force of a successful biobank
7 7 The garbage in - garbage out challenge Garbage samples Perfect samples Perfect Biobank Garbage biobank Garbage Results Garbage Results Perfect samples Perfect Biobank Perfect Results In all cases, the main issue is correct annotation of sample quality
8 8 Improving operations through quality standards and improved technologies Certification by different ISOstandards? Accreditation? Best Biobank Practices? More advanced technologies? Find the best alternative for your biobank favoring maintained and improved quality
9 9 Sample collection 30 min 0-12 hours 37 O C 4 O C...
10 10 In-house sample processing and storage 8 hours 4 O C -20 O C/-80/-196
11 11 Sample retrieval and delivery Throughput Quality assessments Plating/Shipment
12 12 High-throughput automation for storage and retrieval Proven concept at -20 o C -80 o C -150 o C (?) Customized solutions
13 13 Automation at ultralow temperatures Temp below C LN2 gas phase Simple in design With courtesy of Liconic Instruments
14 14 Semi-Automated Biobank storage (SAB) With courtesy of Liconic Instruments
15 15 Labware developments
16 16 Simple, but significant improvements
17 17 Realistic or too futuristic?
18 18 Low-tech,but still significant developments Cutting of a 2 mm thick tissue-slices Snap-freezing between two precooled (LN2) steel plates, the whole instrument sinked into LN2
19 19 Analytic platforms In-house solutions may favor Reduced costs Less analytic variability Reduced sample consumption New omics- platforms Biobank friendly Focus on pre-analytic requirements
20 20 Energy consumption and recycling
21 21 Other challenges Improved annotation of samples Traceability Inventories/catalogues Biobank registries Big data Data access, storage and processing
22 22 Future steps
23 23 Analytic platform A scientific evidence based Evaluation Platform for biobank processes
24 24 BBMRI-LPC - WP 9 To establish a research program on the sample handling processes through the whole production chain used by service providers (biobanks) and balanced with customers demands on costs, speed, versatility and sample quality. To organize national and pan-european sample quality and sustainability studies for different biological materials e.g. serum, plasma, cells etc. Develop a protocol for sample quality assessment Both of the above activities will have a special focus on pre-analytic the quality requirements from various high-throughput -omics platforms To find the right balance between improved technological solutions, quality and costs
25 25 Finally Biobanks build on public trust, and high ethical awareness must govern all biobank processes to protect the safety, integrity and autonomy of sample donors
26 Thank you
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