RECOMMENDED METHODS OF ANALYSIS AND SAMPLING CXS Adopted in 1999.

Transcription

1 RECOMMENDED METHODS OF ANALYSS AND SAMPLNG CXS Adopted in The most updated version of the method should be used, in application of SO/EC The present list of methods reflects the amendments adopted by the 40 th Session of the Codex Alimentarius Commission in 2017.

2 CXS Table of Contents 1. PART A METHODS OF ANALYSS BY COMMODTY CATEGORES AND NAMES All Foods Cereals, Pulses and Legumes and Derived Products Cocoa Products and Chocolate Fats and Oils and Related Products Fish and Fishery Products Foods for Special Dietary Uses Fruit Juices Milk and Milk Products Natural Mineral Waters Processed Fruits and Vegetables Processed Meat and Poultry Products and Soups and Broths Quick Frozen Fruits and Vegetables Spices and Culinary Herbs Sugars and Honey Miscellaneous Products 2. PART B METHODS OF SAMPLNG BY COMMODTY CATEGORES AND NAMES

3 CXS PART A METHODS OF ANALYSS BY COMMODTY CATEGORES AND NAMES Commodity Provision Method Principle Type All Foods All foods Acesulfame K, Aspartame EN All foods Cyclamate EN High performance liquid chromatography High performance liquid chromatography All foods Cyclamate NMKL 123 Spectrophotometry All foods Saccharin EN High performance liquid chromatography All Foods (see also meat products) Nitrates and/or Nitrites EN Part 1- General considerations N/A ndividual Foods 2 ndividual Foods 3 Sulphites Sulphites Cereals, Pulses and Legumes and Derived Products EN AOAC EN NMKL 135 Part 1: Optimized Monier-Williams method Part 2: Enzymatic method Certain pulses Moisture SO 665 Degermed maize (corn) meal and maize (corn) grits Degermed maize (corn) meal and maize (corn) grits Degermed maize (corn) meal and maize (corn) grits Degermed maize (corn) meal and maize (corn) grits Ash AOAC SO 2171 CC Method No 104/1 Fat, crude AOAC F; C (ether extraction) Moisture SO 712 CC Method No 110/1 Particle size (granularity) AOAC Sieving 2 Hominy, fruit juice, sea food 3 Wine, dried apples, lemon juice, potato flakes, sultanas, beer

4 CXS Cereals, Pulses and Legumes and Derived Products Degermed maize (corn) meal and maize (corn) grits Durum wheat semolina and durum wheat flour Durum wheat semolina and durum wheat flour Durum wheat semolina and durum wheat flour Protein CC Method No 105/1 Titrimetry, Kjeldahl digestion Ash (semolina) AOAC SO 2171 Moisture SO 712 CC 110/1 Protein (N x 5.7) CC 105/1 Titrimetry, Kjeldahl digestion nstant Noodles Extraction of oil from instant described in the standard noodles nstant Noodles Acid Value described in the standard Titrimetry nstant Noodles Moisture described in the standard Maize (corn) Moisture SO 6540 Peanuts (raw) Aflatoxins, total AOAC mmunoaffinity column (Aflatest) Peanuts (raw) Aflatoxins, total AOAC Thin layer chromatography Peanuts (intended for further processing) Peanuts (Cereals, shell-fruits and derived products ( including peanuts)) Peanuts (intended for further processing) Aflatoxins, total AOAC Romer minicolmn Sum of aflatoxins B1, B2, G1 and G2 EN SO HPLC with post column derivatization and immunoaffinity column clean up Aflatoxins, total AOAC Holaday-Velasco minicolumn Pearl millet flour Ash AOAC Pearl millet flour Colour Modern Cereal Chemistry, 6th Ed., D.W. Kent-Jones and A.J. Amos (Ed.), pp , Food Trade Press Ltd, London, Colorimetry using specific colour grader Pearl millet flour Fat, crude AOAC F; C (ether extraction) Pearl millet flour Fibre, crude SO 5498: (B.5 Separation) Pearl millet flour Moisture SO 712: CC 110/1 V

5 CXS Cereals, Pulses and Legumes and Derived Products Pearl millet flour Protein AOAC Titrimetry, Kjeldahl digestion Sorghum flour Ash AOAC SO 2171 CC 104/1 Sorghum flour Colour Modern Cereal Chemistry, 6th Ed., D.W. Kent-Jones and A.J. Amos (Ed.), pp , Food Trade Press Ltd, London, Colorimetry using specific colour grader Sorghum flour Fat, crude AOAC F; C (ether extraction) Sorghum flour Fibre, crude CC 113 SO 6541 Sorghum flour Moisture SO 712 CC 110/1 Sorghum flour Particle size (granularity) AOAC Sieving Sorghum flour Protein CC 105/1 Titrimetry, Kjeldahl digestion Sorghum flour Tannins SO 9648 Spectrophotometry Sorghum grains Ash AOAC SO 2171 CC 104/1 Sorghum grains Fat, crude AOAC F, C (ether extraction) Sorghum grains Moisture SO 6540 Sorghum grains Protein CC 105/1 Titrimetry, Kjeldahl digestion Sorghum grains Tannins SO 9648 Spectrophotometry Soy protein products Ash AOAC SO 2171: (Method B) Soy protein products Fat CAC/RM 55 - Method 1 (extraction) Soy protein products Fibre, crude SO 5498 Soy protein products Moisture AOAC (vacuum oven) Soy protein products Protein AOAC D (using factor 6.25) Titrimetry, Kjeldahl digestion V

6 CXS Cereals, Pulses and Legumes and Derived Products Vegetable protein products Ash AOAC SO 2171 (Method B), Direct Vegetable protein products Fat CAC/RM 55 - Method 1 (extraction) Vegetable protein products Fibre, crude AACC Ceramic fiber filteration Vegetable protein products Moisture AOAC (vacuum oven) Vegetable protein products Protein AOAC D (using factor 6.25) Titrimetry, Kjeldahl digestion Wheat flour Ash AOAC SO 2171 CC 104/1 Wheat flour Fat acidity AOAC Titrimetry Wheat flour Moisture SO 712: CC 110/1 Wheat flour Particle size (granularity) AOAC Sieving Wheat flour Protein CC 105/1 Titrimetry, Kjeldahl digestion Wheat protein products including wheat gluten Wheat protein products including Wheat gluten Protein Vital wheat gluten and devitalized wheat gluten AOAC (wheat protein in grain N x 5.7) Solubilized wheat protein AOAC (wheat protein in flour N x 5.7) Kjeldahl Kjeldahl Fibre, crude AOAC Ceramic fiber filteration Wheat protein products including Wheat gluten Ash AOAC SO 2171: method B Whole and decorticated pearl millet grains Ash AOAC

7 CXS Cereals, Pulses and Legumes and Derived Products Whole and decorticated pearl millet grains Whole and decorticated pearl millet grains Whole and decorticated pearl millet grains Whole and decorticated pearl millet grains Fat, crude AOAC F; C (ether extraction) Fibre, crude SO 5498 (B.5 Separation) Moisture SO 712 CC 110/1 Protein AOAC Titrimetry, Kjeldahl digestion Whole maize (corn) meal Ash AOAC SO 2171 CC 104/1 Whole maize (corn) meal Fat, crude AOAC F; C (ether extraction) Whole maize (corn) meal Moisture SO 712 CC 110/1 Whole maize (corn) meal Particle size (granularity) AOAC Sieving Whole maize (corn) meal Protein CC 105/1 Titrimetry, Kjeldahl digestion Cocoa Products and Chocolate Chocolate and chocolate products Cocoa butter AOAC OCCC 14 (Soxhlet extraction) Chocolate and chocolate products Fat-free cocoa solids AOAC Oven evaporation and factor Chocolate and chocolate products Fat-free milk solids OCCC 17 or AOAC Titrimetry, Kjeldahl digestion; after extraction of milk proteins Chocolate and chocolate products Fat, total AOAC (Soxhlet extraction) Chocolate and chocolate products Milkfat OCCC 5 AOAC ; B; Chocolate and chocolate products Moisture OCCC 26 or AOAC (Karl Fischer method); or AOAC or OCCC 1 Titrimetry/Distillation

8 CXS Cocoa Products and Chocolate Chocolate and chocolate products Cocoa (Cacao) Mass or Cocoa/ Chocolate Liquor, and Cocoa Cake Cocoa (Cacao) Mass or Cocoa/ Chocolate Liquor, and Cocoa Cake Non-cocoa butter vegetable fat AOCS Ce 10/02 and described in the Standard Described in the Standard Cocoa shell AOAC and Spiral vessel count, Stone cell count Fat AOAC or OCCC 14 (Soxhlet extraction) Cocoa butter Free fatty acids SO 660 or AOCS Cd 3d-63 Titrimetry Cocoa butter Unsaponifiable matter SO 3596 or SO or AOCS Ca 6b-53 Titrimetry after extraction with diethyl ether Cocoa powders (cocoa) and dry cocoa-sugar mixtures Moisture OCCC 26 or AOAC (Karl Fischer method) Fats and Oils and Related Products Fats and Oils (all) Arsenic AOAC (Codex general method) Colorimetry (diethyldithiocarbamate) Fats and Oils (all) Arsenic AOAC (Codex general method) Colorimetry (molybdenum blue) Fats and Oils (all) Arsenic AOAC (Codex general method) Atomic absorption spectrophotometry Fats and oils Butylhydroxyanisole, butylhydroxytoluene, tertbutylhydroquinone, & propyl gallate AOAC ; or AOCS Ce-6-86 Liquid chromatography Fats and Oils (all) nsoluble impurities SO 663 Fats and Oils (all) Lead AOAC SO (Codex general method) or AOCS Ca 18c-91 Atomic absorption spectrophotometry (direct graphite furnace) Fats and Oils (all) Matter volatile at 105 C SO 662 (open-drying) Fats and Oils (all) Soap content BS 684 Section 2.5; or AOCS Cc Fats and oils not covered by individual standards Acid Value SO 660; or AOCS Cd 3d-63 Titrimetry

9 CXS Fats and Oils and Related Products Fats and oils not covered by individual standards Copper and ron AOAC SO 8294 or AOCS Ca 18b-91 (Codex general method) Atomic absorption Spectrophotometry (direct graphite furnace) Fats and oils not covered by individual standards Peroxide value AOCS Cd 8b-90 SO 3960 Titrimetry using iso-octane Fat spreads and blended spreads Fat content SO DF 194 Fish oils Fatty acid composition SO 5508 Gas chromatography Fish oils Fatty acid composition SO Gas chromatography Fish oils Fatty acid composition AOCS Ce 1b-89 GLC Fish oils Fatty acid composition AOCS Ce 1-07 Capillary GLC Fish oils Fatty acid composition AOCS Ce 2b-11 Alkali hydrolysis Fish oils Fatty acid composition AOCS Ce 1a-13 Capillary GLC Fish oils Fatty acid composition AOCS Ce 2-66 Preparation of methyl esters by fatty acids Fish oils Acid value AOCS Ca 5a-40 AOCS CD 3D-63 SO 3960 NMKL 38 Fish oils Peroxide value AOCS Cd 8b-90 SO 3960 NMKL 158 Fish oils Peroxide value European Pharmacopeia (Part B sooctane as solvent) Fish oils Phospholipids USP-FCC10 2S(Krill oil): Phospholipids, Nuclear Magnetic Resonance, Appendix C Fish oils P-Anisidine value European Pharmacopoeia / AOCS Cd 18-90/ SO 6885 Titration Titration Titration NMR Spectroscopy Spectrophotometry V

10 CXS Fats and Oils and Related Products Fish oils Triglycerides USP 40-NF35(Omega-3 Acid Triglycerides): Content of oligomers and partial glyceride European Pharmacopoeia 1352 (Omega3 acid triglycerides): Oligomers and partial glycerides Fish oils Vitamin A European Parharmcopeia Monograph on Cod Liver Oil (Type A), monograph 01/2005:1192, with LC end-point Fish oils Vitamin A EN (Determination of vitamin A by high performance liquid chromatograph Part 1: Measurement of all-e-retinol and 13- Z-retinol Fish oils Vitamin D EN (Determination of vitamin D by high performance liquid chromatography Measurement of cholecalciferol (D3) or ergocalciferol (D2)) Fish oils Vitamin D NMKL 167 (Cholecalciferol (vitamin D3) and Ergocalciferol (vitamin D2). Determination by HPLC in foodstuffs HPLC-R HPLC R AOCS Cd 11d-96 HPLC-ELSD Named Animal Fats Acidity SO 660; or AOCS Cd 3d-63 Titrimetry Named Animal Fats Copper and ron AOAC SO 8294; or AOCS Ca 18b-91 (Codex general method) Named Animal Fats GLC ranges of fatty acid composition SO 5508 and SO or AOCS Ce 2-66 and Ce 1e-91 or Ce 1f-96 Named Animal Fats odine value (V) SO 3961; or AOAC ; or AOCS Cd 1d-92 Named Animal Fats Peroxide value AOCS Cd 8b-90 SO 3960 Named Animal Fats Relative density SO/AOCS method for apparent density to be inserted LC LC LC LC Atomic absorption Spectrophotometry (direct graphite furnace) Gas chromatography of methyl esters Wijs-Titrimetry Titrimetry using iso-octane Pycnometry

11 CXS Fats and Oils and Related Products Named Animal Fats Refractive index SO 6320; or AOCS Cc 7-25 Refractometry Named Animal Fats Saponification value SO 3657; or AOCS Cd 3-25 Titrimetry Named Animal Fats Unsaponifiable matter SO 3596 or SO 18609; or AOCS Ca 6b-53 Titrimetry after extraction with diethyl ether Named Animal Fats Titre SO 935; or AOCS Cc Thermometry Named Vegetable Oils Acidity SO 660; or AOCS Cd 3d-63 Titrimetry Named Vegetable Oils Apparent density SO 6883, with the appropriate conversion factor; or AOCS Cc 10c-95 Named Vegetable Oils Baudouin test (modified Villavecchia or sesameseed oil test) Pycnometry AOCS Cb 2-40 Colour reaction Named Vegetable Oils Carotenoids, total BS 684 Section 2.20 Spectrophotometry Named Vegetable Oils Copper and iron SO 8294; or AOAC ; or AOCS Ca AAS 18b-91 Named Vegetable Oils Crismer value AOCS Cb 4-35 and AOCS Ca 5a-40 Turbidity Named Vegetable Oils GLC ranges of fatty acid composition SO 5508 and SO ; or AOCS Ce 2-66 and Ce or Ce 1h-05 Gas chromatography of methyl esters Named Vegetable Oils Halphen test AOCS Cb 1-25 Colorimetry Named Vegetable Oils nsoluble impurities SO 663 Named Vegetable Oils odine value (V) Wijs - SO 3961; or AOAC ; or AOCS Cd 1d-92; or NMKL 39 Named Vegetable Oils Lead AOAC ; or SO 12193; or Named Vegetable Oils Moisture & volatile matter at 105 C Wijs-Titrimetry 4 Atomic Absorption AOCS Ca 18c-91 SO 662 Named Vegetable Oils Peroxide value (PV) AOCS Cd 8b-90 or SO 3960 Titrimetry Named Vegetable Oils Refractive index SO 6320 or AOCS Cc 7-25 Refractometry 4 t is possible to calculate the odine Value from fatty acid composition data obtained by gas chromatography e.g. using AOCS Cd 1b-87

12 CXS Fats and Oils and Related Products Named Vegetable Oils Reichert value and Polenske value AOCS Cd 5-40 Titrimetry Named Vegetable Oils Relative density UPAC with the appropriate conversion factor See comment above (Named Animal Fats) 5 Pycnometry Named Vegetable Oils Saponification value (SV) SO 3657 or AOCS Cd 3-25 Titrimetry Named Vegetable Oils Slip point SO 6321 for all oils; AOCS Cc 3b-92 for all oils except palm oils; AOCS Cc 3-25 for palm oils only Open ended capillary tube Named Vegetable Oils Soap content BS 684 Section 2.5; or AOCS Cc Named Vegetable Oils Sterol content SO 12228; or AOCS Ch 6-91 Gas chromatography Named Vegetable Oils Tocopherol content SO 9936 HPLC or AOCS Ce 8-89 Named Vegetable Oils Unsaponifiable matter SO 3596; or SO 18609; or AOCS Ca 6b-53 Olive Oils and Olive Pomace Oils Absorbency in ultra-violet CO/T.20/Doc. No. 19 or SO 3656 or AOCS Absorption in ultra violet Ch 5-91 Olive Oils and Olive Pomace Oils Acidity, free (acid value) SO 660 Titrimetry or AOCS Cd 3d-63 Olive Oils and Olive Pomace Oils Alpha-tocopherol SO 9936 HPLC Olive Oils and Olive Pomace Oils Difference between the actual and theoretical ECN 42 triglyceride content CO/T.20/Doc. no. 20 or AOCS Ce 5b-89 Analysis of triglycerides of HPLC and calculation Olive Oils and Olive Pomace Oils Erythrodiol + uvaol CO/T.20/Doc.no. 30 Gas chromatography Olive Oils and Olive Pomace Oils Halogenated solvents, traces CO/T.20/Doc. no. 8 Gas chromatography Olive Oils and Olive Pomace Oils nsoluble impurities in light SO 663 petroleum Olive Oils and Olive Pomace Oils odine value SO 3961 or AOAC or AOCS Cd 1d- Wijs-Titrimetry 92 or NMKL 39 Olive Oils and Olive Pomace Oils ron and copper SO 8294 or AOAC AAS 5 The method is no longer available.

13 CXS Fats and Oils and Related Products Olive Oils and Olive Pomace Oils Lead AOAC or SO or AOCS Ca AAS 18c-91 Olive Oils and Olive Pomace Oils Moisture and volatile matter SO 662 Olive Oils and Olive Pomace Oils Organoleptic characteristics CO/T.20/Doc. no. 15 Panel test Olive Oils and Olive Pomace Oils Peroxide value SO 3960 or AOCS Cd 8b-90 Titrimetry Olive Oils and Olive Pomace Oils Relative density UPAC 2.101, with the appropriate Pycnometry conversion factor. See comment above Olive Oils and Olive Pomace Oils Refractive index SO 6320 or AOCS Cc 7-25 Refractometry Olive Oils and Olive Pomace Oils Saponification value SO 3657 or AOCS Cd 3-25 Titrimetry Olive Oils and Olive Pomace Oils Sterol composition and total sterols CO/T.20/Doc. no. 30 SO or AOCS Ch 6-91 Gas chromatography Olive Oils and Olive Pomace Oils Stigmastadienes COl/T.20/Doc. no. 11 or SO or Gas chromatography AOCS Cd Olive Oils and Olive Pomace Oils Stigmastadienes SO HPLC Olive Oils and Olive Pomace Oils Trans fatty acids content CO/T.20/Doc no. 17 or SO or AOCS Gas chromatography of methyl esters Ch 2a-94 Olive Oils and Olive Pomace Oils Unsaponifiable matter SO 3596 or SO or AOCS Ca 6b-53 Olive Oils and Olive Pomace Oils Wax content CO/T.20/Doc. no. 18 or AOCS Ch 8-02 Gas chromatography Fish and Fishery Products Fish and fishery products Histamine AOAC Fluorimetry Fish and fishery products Mercury AOAC Flameless atomic absorption spectrophotometry Fish and fishery products: canned products Fish and fishery products: canned products Boiled Dried Salted Anchovies Drained weight Described in the Standard Weighing Net weight Described in the Standard Weighing Sodium Chloride (chloride expressed as sodium chloride) AOAC Titrimetry Canned shrimps or prawns Size, determination of Described in the Standard Number per 100 g

14 CXS Fish and Fishery Products Fish Sauce total nitrogen AOAC digestion Fish Sauce amino acid nitrogen AOAC and AOAC determining formaldehyde titration method subtracting by ammoniacal nitrogen (magnesium oxide method) Fish Sauce ph AOAC The ph shall be measured in a sample of fish sauce diluted with water to 1:10 using a ph meter. The dilution of fish sauce is necessary because of the high ionic strength in the undiluted sauce. electrometry Fish Sauce sodium chloride AOAC potentiometry Fish Sauce sodium chloride AOAC titrimetry V Fish Sauce histamine AOAC Fluorimetry Frozen abalone (covered by glaze) Net weight AOAC Frozen fish and fishery products Quick frozen blocks of fish fillet, minced fish flesh and mixtures of fillets and minced fish flesh Quick frozen blocks of fish fillet, minced fish flesh and mixtures of fillets and minced fish flesh Quick frozen blocks of fish fillet, minced fish flesh and mixtures of fillets and minced fish flesh Quick frozen fish fillets Quick Frozen Fish sticks (fish fingers) and fish portions - breaded or in batter Thawing and cooking procedures Proportion of fish fillet and minced fish Net content of frozen fish blocks covered by glaze Described in the Standards Thawing and heating AOAC Physical separation Described in the Standard Sodium chloride AOAC (Codex general method) Potentiometry Net weight of products covered by glaze Fish content (declaration) Described in the Standard Water spraying and sieving AOAC and calculation (described in the standard)

15 CXS Fish and Fishery Products Quick frozen fish sticks (fish fingers) and fish portions - breaded or in batter Quick Frozen Fish Sticks (fish fingers) and Fish Portions-Breaded and in Batter (except for certain fish species with soft flesh) Quick frozen fish sticks (fish fingers) and fish portions - breaded or in batter Salted Atlantic Herring and Salted Sprat Net weight Described in the Standard Weighing Proportion of fish fillet and minced fish WEFTA Method (described in the Stnadard) Sodium chloride AOAC (Codex general method) Potentiometry Water content AOAC B air drying Salted Fish of the Gadidae Family Salt Described in CXS Titrimetry (Mohr) Salt determined as chloride expressed as sodium chloride Salted Fish and Dried Salted Fish of the Gadidae Family of Fishes Smoked Fish, Smoke-Flavoured fish and Smoke-dried fish Salt Content Water content Sampling and method described in the Standard Water phase salt AOAC AOAC Described in standard 6 Calculation Water activity NMKL 168 SO Electrometry Smoked Fish, Smoke-Flavoured fish and Smoke-dried fish Sturgeon Caviar Salt content Described in CXS Titrimetry (Mohr) Salt determined as chloride expressed as sodium chloride Live and raw bivalve molluscs Paralytic shellfish toxicity AOAC Mouse bioassay V Live and raw bivalve molluscs Paralytic shellfish toxicity AOAC Receptor binding assay V Method Performance Criteria for histamine for fish and fishery products Provision ML (mg/100 g) Minimum applicable range (mg/100 g) LOD (mg/100 g) LOQ (mg/100 g) RSDR(%) Recovery Applicable methods that meet the criteria Histamine 10 (average) AOAC NMKL 99, NMKL 196, Principle Fluorometric HPLC 6 % salt 100 / (%water + %salt)

16 CXS Histamine 20 (each unit) AOAC NMKL 99, NMKL 196, Fluorometric HPLC Determination of Biotoxins in live and raw bivalve molluscs The method selected should be chosen on the basis of practicability and preference should be given to methods which have applicability for routine use. Criteria for determination of Toxin Analogues by chemical methods Methods shall meet the numerical criteria listed in Table 1 and may either meet the minimum applicable range, or LOD and LOQ criteria listed.

17 CXS Table 1. Criteria for determination of Toxin Analogues by Chemical Methods Toxin Group Toxin Minimum applicable range (mg/kg) STX Group Saxitoxin LOD (mg/kg) LOQ (mg/kg) Precision (RSDR) (%) No more than Recovery percent % AOAC (STX) NEO % dcstx % GTX % GTX % GTX % GTX % GTX % GTX % dcgtx % dcgtx % C % C % C % C % Applicable methods that meet the criteria NMKL 182, EN AOAC NMKL 197 OA Group OA % See reference below DTX % DTX % Domoic Acid DA % AZA Group AZA % See reference below AZA % AZA % Reference: Harmonised-SOP-LCMS-OA- Version4.pdf Total toxicity is estimated as the sum of the molar concentrations of detected analogs multiplied by the relevant specific toxicity equivalency factors (TEFs). nternationally scientifically validated TEFs must be used. The science behind TEFs is developing. Current internationally validated TEF s will be found on the FAO website. nformation on TEFs could be incorporated in this standard at a future date. Methods should be validated and used for the relevant toxin analogues that may contribute to total toxicity. Currently known toxin analogues to consider are listed in Table 1. Where toxin analogues that are not listed in Table 1 are determined the competent authority must assess the contribution of these analogs to total toxicity whilst conducting further investigations.

18 CXS Foods for Special Dietary Uses Special foods Ash AOAC Special foods Calcium AOAC CP emission spectrometry Special foods Calories by calculation Method described in CAC/VOL X-Ed.1, Part Special foods Carbohydrates Method described in CAC/VOL X-Ed.1, Part Calculation method Calculation Special foods Chloride AOAC (Codex general method) Potentiometry Special foods Dietary fibre, total AOAC (enzymatic digestion) Special foods Fat CAC/RM 55 (extraction) Special foods Fat in foods not containing starch, meat or vegetable products CAC/RM 1, B-2 Special foods Fill of containers CAC/RM 46 Weighing Special foods Folic acid AOAC Microbioassay Special foods Linoleate (in the form of glycerides) AOAC ; ; Acid hydrolysis, preparation of methyl esters and gas chromatography Special foods Linoleate (in the form of glycerides) AOAC ; Acid hydrolysis and spectrophotometry Special foods Loss on drying (milk based) AOAC SO 6731 DF 21 Special foods Nicotinamide for foods not based on milk AOAC Colorimetry Special foods Nicotinamide for milk-based foods AOAC Microbioassay Special foods Pantothenic acid/enriched foods AOAC Microbioassay Special foods Pantothenic acid/non-enriched foods The Analyst 89 (1964):1, 3-6, ibid. 232 US Dept Agr., Agr. Handbook 97 (1965) Microbioassay V Special foods Phosphorous AOAC Colorimetry (molybdovanadate)

19 CXS Foods for Special Dietary Uses Special foods Protein efficiency ratio (PER) AOAC Rat bioassay Special foods Protein, crude Method described in CAC/VOL X-Ed. 1,Part Titrimetry, Kjeldahl digestion Special foods Riboflavin AOAC Fluorometry Special foods Sodium and Potassium SO 8070 DF 119 Flame atomic absorption spectrometry Special foods Sodium and potassium AOAC CP emission spectrometry Special foods Vitamin A AOAC Colorimetry V Special foods Vitamin A in foods in which carotenes have been added as a source of vitamin A AOAC Spectrophotometry Special foods Vitamin B12 AOAC Microbioassay Special foods Vitamin B6 AOAC Microbioassay Special foods Vitamin C AOAC Microfluorometry Special foods Vitamin C AOAC Colorimetry (dichloroindophenol) Special foods Vitamin D AOAC Rat bioassay V Special foods Vitamin D (D3, milk based infant formula) AOAC Liquid chromatography Special foods Vitamin E AOAC Colorimetry V Special foods Vitamin E (milk based infant formula) AOAC Liquid chromatography Special foods Sodium and Potassium SO 8070 DF 119 Flame atomic absorption spectrometry Follow-up formula Dietary fibre, total AOAC (enzymatic digestion) Follow-up formula odine (milk based formula) AOAC on-selective potentiometry Follow-up formula Pantothenic acid AOAC Measures total pantothenate (free pantothenic acid + CoA- + ACP-bound) and measured as D-pantothenic acid (or calcium D-pantothenate) Microbioassay

20 CXS Foods for Special Dietary Uses Follow-up formula Vitamin A AOAC Colorimetry V Follow-up formula Vitamin A (retinol isomers) AOAC HPLC Follow-up formula Vitamin A (retinol) (above 500 U/l milk after reconstitution) Follow-up formula Vitamin K AOAC EN (vitamin K1) Foods with low-sodium content (including salt substitutes) Foods with low-sodium content (including salt substitutes) Gluten-free foods AOAC HPLC (Measures either aggregated cis + trans K1 or can measure individual cis and trans forms depending on LC column.) HPLC with C30 column to separate the cis- and the trans- K vitamins odine AOAC Titrimetry Silica (colloidal, calcium silicate) AOAC N V Gluten Enzyme-Linked mmunoassay R5 Mendez (ELSA) Method Eur J Gastroenterol Hepatol 2003; 15: nfant formula Biotin EN (d-biotin) (Measures total D-biotin (free + D-biocytin) nfant formula Calories (by calculation) Method described in CAC/Vol X-Ed.1, Part 7 mmunoassay HPLC Calculation 7 Section 9. Calories by calculation Section 9.2 Conversion Factors (a) protein 4 kcal per g (b) carbohydrate 4 kcal per g (c) fat 9 kcal per g (d) monosaccharides 3.75 kcal per g (e) specific food ingredients See Energy and Protein Requirements (FAO Nutrition Meeting Report Series No. 52 or WHO Technical Report Series No. 522) (f) other specific calorie conversion factors maybe used where the formulation of the food and the nutrient content are known and where such specific conversion factors are physiologically more meaningful than the factors listed above

21 CXS Foods for Special Dietary Uses nfant formula Calcium SO 8070 DF 119 Flame atomic absorption spectrophotometry nfant formula Calcium AOAC Flame atomic absorption spectroscopy nfant formula Calcium AOAC CP emission spectroscopy nfant formula Chloride AOAC Potentiometry nfant formula Choline AOAC Enzymatic Colorimetric Method with limitations on applicability due to choline and ascorbate concentration. nfant formula Copper AOAC Flame atomic absorption spectroscopy nfant formula Copper AOAC CP emission spectroscopy nfant formula Chromium (Section B of CXS only) EN Graphite furnace atomic absorption after dry ashing nfant formula Chromium (Section B of CXS only) EN Graphite furnace AAS after pressure digestion nfant formula Chromium (Section B of CXS only) AOAC CP emission spectroscopy nfant formula Chromium (Section B of CXS only) AOAC SO DF 235 CP-MS nfant formula Crude protein 8 SO DF 20-1 Titrimetry (Kjeldahl) nfant formula Fatty acids (including trans fatty acid) AOAC Gas chromatography nfant formula Fatty acids (including trans fatty acid) AOCS Ce 1i-07 Gas chromatography 8 Determination of Crude Protein The calculation of the protein content of infant formulas prepared ready for consumption may be based on N x 6.25, unless a scientific justification is provided for the use of a different conversion factor for a particular product. The value of 6.38 is generally established as a specific factor appropriate for conversion of nitrogen to protein in other milk products, and the value of 5.71 as a specific factor for conversion of nitrogen to protein in other soy products

22 CXS Foods for Special Dietary Uses nfant formula Folic acid AOAC (Measures free folic acid + free, unbound natural folates, aggregated and measured as folic acid) EN (Total folate (free + bound), aggregated and measured as folic acid) nfant formula Folic acid J AOAC nt. 2000:83; (Measures free folic acid + proportion of free, natural folate) nfant formula Folic acid J Chromatogr. A., 928, 77-90, 2001 (Measures total folates after conversion to, and measurement as 5-Me-H4PteGlu) nfant formula odine (for milk-based formula) Microbioassay Optical Biosensor mmunoassay HPLC, incorporating immunoaffinity clean-up and conversion to 5- methyltetrahydrofolate AOAC SO DF 234 CP-MS nfant formula ron 9 AOAC Flame atomic absorption spectrophotometry nfant formula ron AOAC CP emission spectroscopy nfant formula ron AOAC NMKL139 AAS after dry ashing nfant formula Magnesium SO 8070 DF 119 Flame atomic absorption spectrophotometry nfant formula Magnesium AOAC Flame atomic absorption spectroscopy nfant formula Magnesium AOAC CP emission spectroscopy nfant formula Manganese AOAC Flame atomic absorption spectrophotometry nfant formula Manganese AOAC CP emission spectroscopy nfant formula Melamine SO/TS DF/RM 230 LC-MS/MS V V V 9 General Codex methods are also available

23 CXS Foods for Special Dietary Uses nfant formula Molybdenum (Section B of CXS only) nfant formula Molybdenum (Section B of CXS only) Molybdenum (Section B of CXS only) EN Graphite furnace AAS after pressure digestion AOAC CP emission spectroscopy AOAC SO DF 235 CP-MS nfant formula Myo-nositol AOAC SO LC-pulsed amperometry nfant formula Niacin AOAC (niacin (preformed) and nicotinamide) nfant formula Niacin EN (Free and bound and phosphorylated forms measured either as aggregate of nicotinic acid + nicotinamide, or as individual forms) nfant formula Pantothenic acid AOAC SO Microbioassay and turbidimetry HPLC 10 UHPLC-MS/MS nfant formula Phosphorus AOAC Spectrophotometry (molybdovanadate) nfant formula Phosphorus AOAC CP emission spectroscopy nfant formula Riboflavin AOAC Fluorimetry nfant formula Riboflavin EN (Measures natural and supplemental forms, free, bound and phosphorylated (FMN and FAD) aggregated and measured as riboflavin.) nfant formula Selenium AOAC or AOAC Continuous hydride generation Flame atomic absorption spectrometry (HGAAS) nfant formula Selenium EN Hydride generation atomic absorption spectrometry (HGAAS) nfant formula Selenium AOAC CP emission spectroscopy HPLC 10 When published as EN method 11 Care should be taken in the application of the method due to spectral interference

24 CXS Foods for Special Dietary Uses Selenium AOAC SO DF 235 CP-MS nfant formula Sodium and potassium AOAC CP emission spectrometry nfant formula Sodium and potassium SO 8070 DF 119 Flame atomic absorption spectrophotometry nfant formula Thiamine AOAC Fluorimetry nfant formula Thiamine EN (Measures all vitamin B1 forms (natural and added free, bound and phosphorylated) following extraction and conversion to thiamine) nfant formula Total carbohydrates Moisture/Total Solids Ash AOAC nfant formula Total fat AOAC SO 8381 DF 123 nfant formula Total fat for milk-based infant formula (Products not completely soluble in ammonia) AOAC AOAC or AOAC SO 6731 DF 21 HPLC with pre-or post column derivatization to thiochrom Determination by difference (Röse-Gottlieb) SO DF (Weibull-Berntrop) nfant formula Total fatty acids AOAC Gas Chromatography nfant formula Total fatty acids nfant formula Total nucleotides AOAC SO AOAC SO DF231 Gas Chromatography LC 12 Care should be taken in the application of the method due to spectral interference

25 CXS Foods for Special Dietary Uses nfant formula Total phospholipids AOCS Ja7b-91 Gas chromatography with suitable extraction and preparation procedures nfant formula Vitamin A EN (all-trans-retinol and 13-cisretinol) Vitamin A (both natural + supplemental ester forms) aggregated and quantified as individual retinol isomers (13 - cis and alltrans) nfant formula Vitamin A Palmitate (Retinyl Palmitate), Vitamin A Acetate (Retinyl Acetate) AOAC SO nfant formula Vitamin C AOAC SO/DS HPLC-UV nfant formula Vitamin D AOAC D3 measured nfant formula Vitamin D EN (D2 and/or D3 measured as single components. Hydroxylated forms not measured.) NMKL 167 nfant formula Vitamin D AOAC D2 and D3 measured nfant formula Vitamin E AOAC Measures all rac-vitamin E (both natural + supplemental ester forms) aggregated and quantified as α-congeners nfant formula Vitamin E EN (Measures Vitamin E (both natural + supplemental ester forms) aggregated and quantified as individual tocopherol congeners (α, β, γ, δ). HPLC HPLC HPLC HPLC HPLC HPLC HPLC

26 CXS Foods for Special Dietary Uses nfant formula Vitamin E AOAC SO HPLC nfant formula Vitamin B6 AOAC Microbioassay nfant formula Vitamin B6 EN (Aggregates free and bound pyridoxal, pyridoxine and pyridoxamine and measures as pyridoxine) nfant formula Vitamin B6 AOAC EN (Free and bound phosphorylated forms (pyridoxal, pyridoxine and pyridoxamine) converted and measured as pyridoxine) nfant formula Vitamin B6 EN (includes glycosylated forms) (Free and bound phosphorylated and glycosylated forms measured as the individual forms pyridoxal, pyridoxine and pyridoxamine) nfant formula Vitamin B12 AOAC (Measures total vitamin B12 as cyanocobalamin) Microbioassay HPLC HPLC Turbidimetric Method nfant formula Vitamin B12 AOAC SO HPLC nfant formula Zinc AOAC Flame atomic absorption spectroscopy nfant formula Zinc AOAC CP emission spectroscopy

27 CXS Methods of analysis for dietary fibre: Guidelines for Use of Nutrition and Health Claims: Table of Conditions for Claims Standard Provisions Method Principle Type General methods that do not measure the lower molecular weight fraction (i.e. monomeric units<=9) (2) All foods (1) All foods (1) All foods (1) All foods (1) Method applicable for determining dietary fibres that do not include the lower molecular weight fraction. (4) Method applicable for determining dietary fibres that do not include the lower molecular weight fraction and also includes determination for soluble and insoluble dietary fibres (4) Method applicable for determining dietary fibres that do not include the lower molecular weight fraction, in foods and food products containing more than 10% dietary fibres and less than 2% starch (e.g. fruits) (4) Method applicable for determining dietary fibres that do not include the lower molecular weight fraction. Provides sugar residue composition of dietary fibre polysaccharides, as well as content of Klason lignin (4). AOAC AACC ntl AOAC AACC ntl NMKL 129 Enzymatic gravimetry Enzymatic gravimetry Type Type AOAC gravimetry Type AOAC AACC ntl NMKL 162 All foods (1) nsoluble dietary fibres in food and food products (4) AOAC (Specific for insoluble fibre) AACC ntl All foods (1) Soluble dietary fibres in food and food products (4) AOAC (Specific for soluble fibre) Enzymatic GC/ colorimetry gravimetry Enzymatic gravimetry Enzymatic gravimetry General methods that measure both the higher (monomeric units > 9) and the lower molecular weight fraction (monomeric units <=9) (2) Type Type Type All foods (1) Method applicable for determining the content of dietary fibres of higher and lower molecular weight, in food where resistant starches are not present AOAC AACC ntl Enzymatic gravimetry and Liquid chromatography Type All foods (1) Method applicable for determining the content of dietary fibres of higher and lower molecular weight. The method is applicable in food that may, or may not, contain resistant starches. AOAC AACC ntl Enzymatic- High Pressure Liquid Chromatography Type All foods (1) Method applicable for determining the content of insoluble and soluble dietary fibres of higher and lower molecular weight. The method is applicable in food that may, or may not, contain resistant starches AACC ntl AOAC Enzymatic- High Pressure Liquid Chromatography Type

28 CXS Standard Provisions Method Principle Type Methods that measure individual specific components (monomeric units: the whole range for each type of components is covered) (2) All foods (1) (1 3)(1 4) Beta-D-Glucans AOAC AACC ntl All foods (1) All foods (1) Fructans (oligofructoses, inulin, hydrolyzed inulin, polyfructoses, fructooligosaccharides) (applicable to added fructans) Fructans (oligofructoses, inulin, hydrolyzed inulin, polyfructoses, fructooligosaccharides) (not applicable highly depolymerised fructans) AOAC AACC ntl AOAC AACC ntl All foods (1) Polydextrose AOAC AACC ntl All foods (1) Trans-galacto-oligo saccharides AOAC AACC ntl All foods (1) Resistant starch (Recommended for RS3) AOAC AACC ntl Enzymatic Enzymatic & HPAEC- PAD Enzymatic & colorimetric HPAEC-PAD HPAEC-PAD Enzymatic Type Type Type Type Type Type

29 CXS Other methods (2) that have not been subjected to interlaboratory evaluation under AOAC international guidelines Yeast nsoluble glucans and mannans of yeast cell wall (for yeast cell cell wall wall only) Eurasyp (European association for specialty yeast product) LM Bonanno. Biospringer online version : ue.home.screen. All foods Fructo-oligosaccharides (monomeric units<5) Ouarné et al in Complex Carbohydrates in Foods. Edited by S. Sungsoo, L. Prosky & M. Dreher. Marcel Dekker nc, New York All foods Non-starch polysaccharides (NSP) (3) Englyst H.N, Quigley M.E., Hudson G. (1994) Determination of dietary fibre as non-starch polysaccharides with gas-liquid chromatographic high performance liquid chromatographic or spectrophotometric measurement of constituent sugars Analyst 119, Chemical & HPAEC-PAD HPAEC-PAD Gas-Liquid Chromatography Type V Type V Type V (1) Users should consult the description of each method for the food matrices that were the subject of interlaboratory study in the Official methods of Analysis of AOAC nternational. (2) Two issues are left for national authorities: to include monomeric units 3-9 and which isolated or synthetic compounds have physiological benefit. (Refer to the Guidelines for Nutrition Labelling (CXG ). (3) Quantitation lost for resistant starch. Refer to specific methods. (4) Quantitation lost for inulin, resistant starch, polydextrose and resistant maltodextrins. Refer to specific methods.

30 CXS Commodity Provisions Method Principle Type Ascorbic acid-l (additives) FUMA 17A HPLC Ascorbic acid-l (additives) SO Fluorescence spectrometry V Ascorbic acid-l (additives) AOAC ndophenol method FUMA 17 SO Carbon dioxide (additives and FUMA 42 Titrimetry V processing aids) (back-titration after precipitation) Cellobiose FUMA 4 Capillary gas chromatography V Citric acid 13 (additives) AOAC HPLC Citric acid 5 (additives) EN 1137 Enzymatic determination FUMA 22 Glucose and fructose EN HPLC (permitted ingredients) FUMA 67 NMKL 148 Glucose-D and fructose-d EN 1140 Enzymatic determination (permitted ingredients) FUMA 55 HFCS & HS in apple juice Determination of HFCS & HS by Capillary CAP GC Method V (permitted ingredients) GC method JAOAC 84, 486 (2001) Malic acid (additives) AOAC Enzymatic determination and HPLC Malic acid-d EN Enzymatic determination FUMA 64 Malic acid-d in apple juice AOAC HPLC Malic acid-l EN 1138 Enzymatic determination FUMA 21 Pectin (additives) FUMA 26 Precipitation/photometry Benzoic acid and its salts; FUMA 63 HPLC sorbic acid and its salts NMKL 124 Benzoic acid and its salts SO 5518, SO 6560 Spectrometry Preservatives in fruit juices (sorbic acid and its salts) SO 5519 Spectrometry 13 All juices except citrus based juices

31 CXS Quinic, malic & citric acid in cranberry juice cocktail and apple juice (permitted ingredients and additives) Determination of quinic, malic and citric acid in cranberry juice cocktail and apple juice AOAC Saccharin NMKL 122 Liquid chromatography Soluble solids AOAC EN FUMA 8 SO 2173 Sucrose (permitted EN ingredients) Sucrose (permitted ingredients) FUMA 56 EN FUMA 67 NMKL 148 HPLC ndirect by refractometry Enzymatic determination Sulphur dioxide (additives) Optimized Monier Williams AOAC Titrimetry after distillation FUMA 7A NMKL 132 Sulphur dioxide (additives) NMKL 135 Enzymatic determination Sulphur dioxide (additives) SO 5522, SO 5523 Titrimetry after distillation Tartaric acid in grape juice EN HPLC (additives) FUMA 65 Total nitrogen EN FUMA 28 Digestion/titration Sections 3.2 Quality Criteria and 3.3 Authenticity 14 Determination of acetic acid EN 12632; FUMA 66 Determination of alcohol (ethanol) FUMA 52 Detection of anthocyanins FUMA 71 Determination of ash in fruit products AOAC ; EN 1135; FUMA 9 Detection of beet sugar in fruit juices AOAC HPLC Enzymatic determination Enzymatic determination HPLC Deuterium NMR Verification of Composition, Quality and Authenticity Fruit juices and nectars should be subject to testing for authenticity, composition, and quality where applicable and where required. The analytical methods used should be those found in Section 9, Methods of Analysis and Sampling. The verification of a sample s authenticity / quality can be assessed by comparison of data for the sample, generated using appropriate methods included in the standard, with that produced for fruit of the same type and from the same region, allowing for natural variations, seasonal changes and for variations occurring due to processing.

32 CXS Determination of benzoic acid as a marker in orange juice AOAC Determination of C 13 /C 12 ratio of ethanol derived from fruit juices JAOAC 79, No. 1, 1996, Determination of carbon stable isotope ratio of apple juice AOAC JAOAC 64, 85 (1981) Determination of carbon stable isotope ratio of orange juice AOAC Determination of carotenoid, total/individual groups EN 12136; FUMA 59 Determination of centrifugable pulp EN 12134; FUMA 60 Determination of chloride (expressed as sodium chloride) EN FUMA 37 Determination of chloride in vegetable juice AOAC (Codex general method) SO 3634 Determination of essential oils (Scott titration AOAC FUMA 45* Determination of essential oils (in citrus fruit) (volume determination)* SO 1955 Determination of fermentability FUMA 18 Determination of formol number EN 1133 FUMA 30 Determination of free amino acids EN FUMA 57 Determination of fumaric acid FUMA 72 HPLC Stable isotope mass spectrometry Stable isotope mass spectrometry Stable isotope mass spectrometry Spectrophotometry Centrifugation/% value Electrochemical titrimetry Titration (Scott) distillation, titration Distillation and direct reading of the volume determination Microbiological method Potentiometric titration Liquid Chromatography HPLC

33 CXS Determination of glucose fructose and saccharose EN FUMA 67 NMKL 148 Determination of gluconic acid FUMA 76 Determination of glycerol FUMA 77 Determination of hesperidin and naringin EN FUMA 58 Determination of hydroxymethylfurfural FUMA 69 Determination of hydroxymethylfurfural SO 7466 Determination of isocitric acid-d FUMA 54 Determination of Lactic acid- D and L EN FUMA 53 Determination of L-malic/total malic acid ratio in apple juice AOAC Determination of naringin and neohesperidin in orange juice AOAC Determination of ph-value NMKL 179 EN 1132 FUMA 11 SO 1842 Determination of phosphorus/phosphate EN 1136 FUMA No 50 Determination of proline by photometry nonspecific determination EN 1141 FUMA 49 Determination of relative density EN 1131 (1993); FUMA 01 & FU Method No General sheet (1971) Determination of Relative density FUMA 01A HPLC Enzymatic determination Enzymatic determination HPLC HPLC Spectrometry Enzymatic determination Enzymatic determination Enzymatic determination and HPLC HPLC Potentiometry Photometric determination Photometry Pycnometry Densitometry V

34 CXS Determination of sodium, potassium, calcium, magnesium in fruit juices EN 1134 FUMA 33 Determination of sorbitol-d FUMA62 Determination of stable carbon isotope ratio in the pulp of fruit juices ENV Analytica Chimica Acta 340 (1997) Determination of stable carbon isotope ratio of sugars from fruit juices ENV Analytica Chimica Acta.271 (1993) Determination of stable hydrogen isotope ratio of water from fruit juices ENV Determination of stable oxygen isotope ratio in fruit juice water ENV Detection of starch AOAC FUMA 73 Determination of sugar beet derived syrups in frozen concentrated orange juice δ 18 O Measurements in Water AOAC Determination of titrable acids, total EN FUMA 03 SO 750 Determination of total dry matter (vacuumoven drying at 70 C)* EN FUMA 61 Determination of total solids (Microwave oven drying)* AOAC Determination of Vitamin C (dehydro-ascorbic acid and ascorbic acid) AOAC Atomic Absorption Spectroscopy Enzymatic determination Stable isotope mass spectrometry Stable isotope mass spectrometry Stable isotope mass spectrometry Stable isotope mass spectrometry Colorimetric Oxygen isotope ratio analysis Titrimetry Gravimetric determination Gravimetric determination Microfluorometry * Because there is no numerical value in the Standard duplicate Type methods have been included which may lead to different results.

35 CXS Milk and Milk Products Milk products ron NMKL 139 AOAC (Codex general method) Atomic absorption spectrophotometry Milk products ron NMKL 161 / Atomic absorption AOAC spectrophotometry Milk products ron AOAC nductively Coupled Plasma optical emission spectrophotometry Milk products ron SO 6732 DF 103 Photometry (bathophenanthroline) V Milk and Milk Products Melamine SO/TS DF/RM 230 LC-MS/MS V Milk products (products not completely soluble in ammonia) Blend of evaporated skimmed milk and vegetable fat Blend of evaporated skimmed milk and vegetable fat Blend of evaporated skimmed milk and vegetable fat Blend of evaporated skimmed milk and vegetable fat Reduced fat blend of evaporated skimmed milk and vegetable fat Reduced fat blend of evaporated skimmed milk and vegetable fat Reduced fat blend of evaporated skimmed milk and vegetable fat Reduced fat blend of evaporated skimmed milk and vegetable fat Blend of skimmed milk and vegetable fat in powdered form Milk fat SO DF (Weibull-Berntrop) Total fat SO 1737 DF 13 (Röse-Gottlieb) Milk solids-not-fat 15 (MSNF) SO 6731 DF 21 and SO 1737 DF 13 Calculation from total solids content and fat content (Röse-Gottlieb) Milk protein in MSNF 15 SO DF 20-1 Titrimetry (Kjeldahl) V Milk protein in MSNF 15 AOAC 991/20 Titrimetry (Kjeldahl) V Total fat SO 1737 DF 13 (Röse-Gottlieb) Milk solids-not-fat (MSNF) SO 6731 DF 21 and SO 1737 DF 13 Calculation from total solids content and fat content (Röse-Gottlieb) Milk protein in MSNF 15 SO DF 20-1/ Titrimetry (Kjeldahl) V Milk protein in MSNF 15 AOAC Titrimetry (Kjeldahl) V Total fat SO 1736 DF 9 (Röse-Gottlieb) 15 Milk total solids and Milk solids-not-fat (MSNF) content include water of crystallization of lactose

36 CXS Milk and Milk Products Blend of skimmed milk and vegetable fat in powdered form Blend of skimmed milk and vegetable fat in powdered form Blend of skimmed milk and vegetable fat in powdered form Reduced fat blend of skimmed milk powder and vegetable fat in powdered form Reduced fat blend of skimmed milk powder and vegetable fat in powdered form Reduced fat blend of skimmed milk powder and vegetable fat in powdered form Reduced fat blend of skimmed milk powder and vegetable fat in powdered form Blend of sweetened condensed skimmed milk and vegetable fat Blend of sweetened condensed skimmed milk and vegetable fat Blend of sweetened condensed skimmed milk and vegetable fat Blend of sweetened condensed skimmed milk and vegetable fat Blend of sweetened condensed skimmed milk and vegetable fat Reduced fat blend of sweetened condensed skimmed milk and vegetable fat Reduced fat blend of sweetened condensed skimmed milk and vegetable fat Water 16 SO 5537 DF 26, drying at 87 C Milk protein in MSNF 15 SO DF 20-1/ Titrimetry (Kjeldahl) V Milk protein in MSNF 15 Total fat SO 1736 DF 9 (Röse-Gottlieb) Water 16 SO 5537 DF 26, drying at 87 C Milk protein in MSNF 15 SO DF 20-1 Titrimetry (Kjeldahl) V Milk protein in MSNF 15 AOAC Titrimetry (Kjeldahl) V Total fat SO 1737 DF 13 (Röse-Gottlieb) Sucrose SO 2911 DF 35 Polarimetry V Milk solids-not-fat (MSNF) SO 6734 DF 15 Calculation from total solids content, V fat content and sugar content Milk protein in MSNF 15 SO DF 20-1/ Titrimetry (Kjeldahl) V Milk protein in MSNF 15 AOAC Titrimetry (Kjeldahl) V Total fat SO 1737 DF 13 (Röse-Gottlieb) Milk solids-not-fat (MSNF) SO 6734 DF 15 Calculation from total solids content and sugar content V 16 Water content excluding the crystallized water bound to lactose (generally known as moisture content )