Eurofins Carbohydrate Competence Centre: Your partner for sweet analyses

Transcription

1 Eurofins Carbohydrate Competence Centre: Your partner for sweet analyses Dr. Jeroen van Soest BU manager Dr. Yannis Vrasidas Scientific support manager Santiago, Chili,

2 Carbohydrate Competence Centre Expertise BU Manager: Dr. Jeroen van Soest specialist in carbohydrates & food-feed-pharma-non-food products PhD on starch bioplastics > 100 publications/patents/standards in the field winner several prizes e.g. 2 cereal prizes, bioplastic Oskar. CCC lead scientist & co-author: Dr. Yannis Vrasidas carbohydrates and modification prebiotics & dietary fibres & HMOs pharma and food

3 Outline Eurofins Group & Carbohydrate Competence Centre Topic 1 9 Tuesday 9:20-9:40 (SESIÓN PARALELA 5 SALÓN CORDILLERA) Reemplazo de ingredientes críticos por fibra dietaria. Cómo la interacción de ingredientes incide en las mediciones y dificultades observadas. = Replacement of critical ingredients with dietary fiber. How the ingredients affects the measurements and difficulties observed. Topic 2 Tuesday 14:50-15:10 (SESIÓN PARALELA 9 SALÓN CORDILLERA) Un ejemplo: el desarrollo del método de Fibra Dietaria relacionada a alimentos funcionales con ingredientes destinados a reducir nutrientes. Polialcoholes, inulinas, edulcorantes u otros. Apoyo en la legislación Unión Europea. = An example: Dietary Fiber methods related to functional foods with nutrient reducing ingredients, e.g. polyalcohols, inulins, sweeteners or others. Consequences for (European Union) legislation.

4 Eurofins Group Leading international group of laboratories Providing analytical testing services Areas that have strong impact in human health Founded in 1987 Network of 310 laboratories in 39 countries Over 130,000 reliable analytical methods Over 30,000 employees

5 Carbohydrate Competence Centre History & Facts 34 employees, 28 FTE (analysts & scientists) with strong expertise in - Carbohydrate, Food, Analytical Chemistry & Analysis Wide portfolio of routine & specialty analyses - > 80 tests analyses / month - based on (inter)national standard methods (AOAC, ISO, AACC) - in-house methods / national and international clients Development of new methods Member or active in international leading commissions - AOAC / SPIFAN recently 1 st action standard AOAC (Inulin-Fructan) method with Nestle - ISO / IDF / NEN developing new sugar profile with ISO-IDF & industrial partners Strong collaboration with universities & technical colleges

6 Portfolio Main Segments / Markets Segments / Markets Food general Feed Ingredients Dairy (milk, yoghurt, ice cream,..) Infant formula / Baby food Bakery (bread, biscuits, ) & Pasta Beverages (beer, energy drinks, juice, coffee-tea,..) Carbohydrates All kind of carbohydrates, labeling sugars, reducing & digestible carbohydrates, sucralose/alditols Gelatinized starch, galactomannan, hydrocolloids, raff/stach/verb Inulin/FOS, GOS, b-glucans, starch, pectins Lactose (free), hydrocolloids, label & specialty sugars Inulin/FOS, GOS, sialic acid, dietary fibre, sugars incl. galactose, palatinose, lactulose, polydextrose, inositol Starch, resistant starch, dietary fibre, malto-oligosaccharides, betaglucans, pectins, amylose/amylopectin, b-glucans, sweeteners, hydrocolloids, coffee sugars

7 DF Topic 1 Replacement of critical ingredients with dietary fiber. How the ingredients affects the measurements and difficulties observed. Dietary fibres: What? Why? How? see our Folder on DF 9:20-9:40

8 Fibre components

9 What? Markets (examples) Ingredients Final products Retail Products (examples) Bakery Pasta Beverages Dairy Infant & Baby food Healthy food products

10 What? Components / sources DIETARY FIBRE (DF) High Molecular Weight (HMWDF) Resistant starch (RS) Low Molecular Weight (LMWDF and prebiotic) Insoluble HMW (IHMWDF) - Cellulose - Lignin - Hemicellulose (insoluble pentosans) - Insoluble pectin - b-glucans (yeast/moulds) -... Soluble HMW (SHMWDF) - Hydrocolloids - carrageenan - gums (Soluble) pentosans - Soluble pectins - Cereal b-glucan RS 1 Physical inaccessible starch - RS 2 Resistant starch granules - RS 3 Retrograded starch - RS4 Chemically modified starch - Inulin - Fructose oligosaccharide (FOS) - Galactose oligosaccharide (GOS) - Polydextrose - Resistant maltodextrin -...

11 Dietary Fibre Analysis

12 Why do we need (to analyze) DF? Health benefits Blood sugar control; Diabetes Cholesterol control Weight loss & management Reduce risk of heart disease Prebiotic activity Dietary fibre content on product label The more the better

13 Complications by different investigations Different analytical methods applied for quantifying the amount of dietary fibre Possible double estimation of dietary fibre components if different methods combined Country-to-country differences in the dietary fibre composition e.g. South vs Northern European diets regarding the physiological effects Country-to-country differences in legislation UK recommended 18 g/day DF intake (as NSP by the Englyst method) Other countries g/day DF intake (as AOAC fibre)

14 How? DF = Complex landscape total high and low molar weight dietary fibre AOAC RS1 RS2 RS4 Resistant starch AOAC RS3 Classical total dietary fibre AOAC Classical soluble high molar mass dietary fibre AOAC Inulin/FOS AOAC & AOAC Polydextrose AOAC Galactooligosaccharide AOAC Resistant maltodextrin AOAC

15 How? Complexity DF: Various extraction routes DF Pancreatic amylase/amg treatment Protease treatment Ethanol precipitation AOAC Classical dietary fibre AOAC Resistant starch (37 C) RS remains present as DF Residue Filtration Filtrate AOAC DF containing supplemented resistant maltodextrin Gravimetry IHMWDF + SHMWDF Including resistant starch Removal ethanol Desalting GPC-RI LMWDF 2 analytical results

16 AOAC (instead of AOAC ) The new total dietary fibre determination Pancreatic amylase/amg treatment Protease treatment residue filtration filtrate Ethanol precipitation residue filtration filtrate Removal ethanol desalting gravimetry gravimetry GPC-RI IHMWDF + RS1 + RS2 + RS3 + (partly) RS4 SHMWDF + (partly) RS4 LMWDF

17 Dietary fibre and prebiotic analyses Dietary fiber analyses Method Type DF included in the analysis AOAC HMWDF AOAC ihmwdf (incl. RS3) + shmwdf AOAC HMWDF (incl. RS) + LMWDF AOAC ihmwdf (incl. RS) + shmwdf + LMWDF AOAC Resistant starch AOAC b-glucan Prebiotic analyses Method Dedicated prebiotic AOAC Inulin/FOS AOAC Inulin/FOS AOAC Polydextrose AOAC Galacto-oligosaccharides (GOS) AOAC Resistant maltodextrin Specific methods

18 Portfolio Dietary Fibres Analyte Matrix Method based on Classical Dietary Fibre Food AOAC Classical Dietary Fibre (soluble & insoluble) Food AOAC Total Dietary fibre (incl. LMWDF) Food AOAC Total Dietary fibre (incl. LMWDF) Food (high starch content) AOAC revised Total Dietary fibre (soluble, insoluble & LMWDF) Food AOAC Total Dietary fibre (cont. suppl. resistant maltodextrin) Food AOAC Pectin (as uronic acid) Food/Feed in-house b-glucan (cereals/grains) Food/Feed AOAC b-glucan (yeasts/moulds) Ingredients in-house Resistant starch Food/Feed AOAC Various hydrocolloids Food/Feed In-house methods

19 Suggested decision tree for DF analysis Does the sample contain natural low molar weight dietary fibre or added dietary fibre ingredient NO YES, added fibre ingredients YES, natural low molar weight dietary fibres UNKNOWN With natural HMWDF With RS With GOS With Resistant maltodextrin With Inulin/FOS With Polydextrose With RS3 With RS1, RS2 or RS4 AOAC or AOAC AOAC AOAC (+ AOAC if %GOS in DF needed) AOAC AOAC or AOAC

20 Summarizing Various definitions and currently accepted dietary fibre definition (EC directive 2008/100/EC) Which (food) components are considered as dietary fibre Health benefits related to dietary fiber consumption Difficulty to correctly determine all components in the dietary fibre by one method and the disadvantages of combining several methods. Decision tree for dietary fibre analysis

21 Carbohydrate Competence Centre Thank you contact us website

22 Questions