Olios sjostedti Lessert, 1921 (Araneae: Sparassidae): first description of the female and first records from South Africa and Botswana

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1 African Invertebrates Vol. 55 (2) Pages Pietermaritzburg 25 July 2014 Olios sjostedti Lessert, 1921 (Araneae: Sparassidae): first description of the female and first records from South Africa and Botswana Peter Jäger Arachnology, Research Institute Senckenberg, Senckenberganlage 25, D Frankfurt am Main, Germany; ABSTRACT Olios sjostedti Lessert, 1921, originally described from Mount Kilimanjaro, Tanzania, is now recorded from South Africa and Botswana as well. A redescription of the male is provided and the female described for the first time. KEY WORDS: Afrotropical region, South Africa, Botswana, Tanzania, Sparassidae, Olios, Taxonomy, redescription, huntsman spiders. INTRODUCTION The genus Olios Walckenaer, 1837 currently consists of 246 species and six subspecies (Platnick 2014). Jäger and Kunz (2005) diagnosed males in an identification key by their distal embolic coil and the presence of tegular apophyses. Material from South Africa revealed an Olios species with a simple embolus not having a distal coil. It was identified as Olios sjostedti Lessert, 1921, which had been previously described only from Tanzania. A redescription of the male is provided and the female described for the first time. The specimens represent the first records in South Africa and Botswana, and generally the second report of geographical occurrence for this species. The new localities are approximately 2400 to 2700 kilometres southwest to south of the type locality. MATERIAL AND METHODS Material was examined and drawn while in 70 % denatured ethanol utilising a Leica MZ16 stereomicroscope with a camera lucida attachment. Copulatory organs were dissected and kept in micro-vials within the original tubes. Left male palps in ventral view are used for describing the origin points of the embolus, apophyses and conductor on the tegulum. Abbreviations: ALE anterior lateral eyes; AME anterior median eyes; AW anterior width of dorsal shield of prosoma; d dorsal; DK serial field number of material collected by Dirk Kunz; OL opisthosoma length; OW opisthosoma width; p prolateral; PJ serial number for Sparassidae examined by Peter Jäger; PL prosoma length; PLE posterior lateral eyes; PME posterior median eyes; PW prosoma width; r retrolateral; RTA retrolateral tibial apophysis; SD serial number of Sparassidae with tissue/dna samples available; v ventral; I IV referring to leg numbers. Museum collections (curators given in parentheses): MHNG Museum d Histoire Naturelle, Geneva, Switzerland (Peter Schwendinger); NMBA National Museum, Bloemfontein, South Africa (Leon Lotz); NHRM Naturhistoriska Riksmuseet, Stockholm, Sweden (Gunvi Lindberg, Torbjörn Kronestedt); urn:lsid:zoobank.org:pub:03063cc3-ce4b-4c3f-81cf-2dfb6f911806

2 202 AFRICAN INVERTEBRATES, vol. 55 (2), 2014 PPRI Plant Protection Research Institute, Pretoria, South Africa (Ansie Dippenaar- Schoeman, Petro Marais); RMCA Royal Museum for Central Africa, Tervuren, Belgium (Rudy Jocqué); SMF Senckenberg Research Institute, Frankfurt am Main, Germany (Julia Altmann, Peter Jäger). TAXONOMY Sparassidae Bertkau, 1872 Sparassinae Bertkau, 1872 Olios Walckenaer, 1837 Olios sjostedti Lessert, 1921 Figs 1 16 Olios sjöstedti: Lessert 1921: 391, figs (description of male); Roewer 1955: 696. Olios sjœstedti: Bonnet 1958: Olios sjostedti: Platnick [N.B.: according to the ICZN (1999: article ), letters with umlauts, i.e. ö, ü and ä, are only changed to oe, ue and ae, respectively, when based on a German word; since Yngve Sjöstedt ( ) was a Swedish zoologist, the scientific name has to be sjostedti ]. Diagnosis: Small to medium-sized Sparassinae, total length : ; Males can be distinguished from all other Olios spp. by the simple and only slightly curved embolus without a distal coil or bend (Figs 2 3). The arrangement of two tegular apophyses arising proximally from the tegulum in a 6- to 7-o clock position is characteristic (Figs 1 3). Females, like those of many Olios species, are not easy to diagnose. The asymmetrical arrangement of the internal duct system might be one important char acter (Fig. 9). In the cleared epigyne in ventral view, the fertilisation ducts appear as an asymmetrical, heart-shaped structure. The epigyne does not have a distinct median slit and there is only a small posterior incision and dark triangular region around the copulatory openings (Figs 8, 15). Male. Redescription: (PJ 3418): PL 4.0, PW 4.6, AW 2.4, OL 5.0, OW 3.3. Eye diameters: AME 0.31, ALE 0.23, PME 0.21, PLE Eye interdistances: AME AME 0.17, AME ALE 0.19, PME PME 0.41, PME PLE 0.49, AME PME 0.26, ALE PLE 0.25, clypeus height at AME 0.06, clypeus height at ALE Leg measurements: palp 5.3 (1.6, 0.7, 0.9, -, 2.1); leg I 21.7 (5.9, 2.5, 5.9, 5.7, 1.7); leg II 23.5 (6.5, 2.6, 6.4, 6.3, 1.7); leg III 17.0 (5.1, 1.9, 4.6, 4.1, 1.3); leg IV 17.7 (5.2, 1.8, 4.6, 4.7, 1.4). Leg formula: II-I-IV-III. Spination: palp: 130, 000, 0000 (bristles present on patella and tibia); Femur I III 323, IV 320; Patella I IV 000; Tibia I-II, III 2124(3), IV 210(2)4; Metatarsus I III 2024, IV Metatarsus IV with ventral distal spine in sparse distal scopula, metatarsi I III without such a spine. Cheliceral furrow with 2 anterior and 4 posterior teeth, the two median posterior teeth fused; with 5 long bristles at posterior base of fang (Fig. 7). Copulatory organ: As in diagnosis. Embolus and conductor arising from tegulum in 7-o clock position, conductor widened distally (Figs 2 3). Embolus tip slightly widened (Figs 5 6). Two apophyses at base of embolus, the one closer to embolus prolaterad, pointed, and partly hidden behind second apophysis, the proximal one laminar with slightly serrated margin (Figs 1 3). Bulbal parts behind embolus and conductor partially

3 Olios sjostedti from South Africa and Botswana 203 Figs 1 7. Olios sjostedti Lessert, 1921: (1 2, 4 5) holotype male from Tanzania; (3, 6 7) male from South Africa. (1 4) Left male palp: (1) prolateral view, (2 3) ventral view, (4) retrolateral view; (5 6) embolus tip, retrolateral view; (7) left chelicerae, ventral view (bristles are indicated by their insertion points). Abbreviations: drta dorsal part of retrolateral tibial apophysis, E embolus, TA1/2 tegular apophysis 1/2, vrta ventral part of retrolateral tibial apophysis. membranous. Sperm duct only very slightly undulating (Figs 2 3). Cymbium tip as long as or slightly longer than conductor width. RTA complex, with strongly sclerotised and distinctly bent dorsal part and soft, spoon-shaped ventral part (Figs 2 4).

4 204 AFRICAN INVERTEBRATES, vol. 55 (2), 2014 Figs Olios sjostedti Lessert, 1921, female from South Africa: (8) epigyne, ventral view; (9) vulva, dorsal view; (10) schematic course of internal duct system, dorsal view (open circle copulatory orifice, arrow fertilisation duct in direction of the uterus externus); (11) right chelicerae, ventral view, mirrored (bristles are indicated by their insertion points). Abbreviations: CD copulatory duct, FD fertilisation duct, PC posterior coils between spermathecae and fertilisation ducts, SP spermathecae. Colouration in ethanol: Yellowish brown with dark brown patterning (Figs 12 13). Dor sal prosoma with patches behind eye region, one patch at anterior end of fovea. Ventral prosoma including ventral coxae and femora pale yellowish brown, without pattern. Chelicerae only marginally as well as labium and gnathocoxae slightly darker. Legs and palps yellowish brown, darker distally, partly with indistinct dots and small patches. Dorsal opisthosoma with vivid pattern consisting of partly fused patches along heart and more distinctly along posterior midline, with irregular dots laterally. Ventral opisthosoma pale yellowish brown with few dark patches or spots in front of spinnerets.

5 Olios sjostedti from South Africa and Botswana 205 Figs Olios sjostedti Lessert, 1921, (12 13) male and (14 15) female from South Africa: habitus (12, 14) dorsal view; (13, 15) ventral view. Note the ectoparasitic insect larva attached to the anterior opisthosoma on the female (14, arrow). Female. Description: (PJ 3425): PL 3.9, PW 4.4, AW 2.8, OL 7.8, OW 5.3. Eye diameters: AME 0.26, ALE 0.23, PME 0.23, PLE Eye interdistances: AME AME 0.20, AME ALE 0.21, PME PME 0.40, PME PLE 0.53, AME PME 0.23, ALE PLE 0.30, clypeus height at AME 0.11, clypeus height at ALE Leg measurements: palp 4.1 (1.2, 0.5, 0.9, -, 1.5); leg I 15.5 ( , 3.9, 3.9, 1.3); leg II 16.7 (4.8, 2.1, 4.3, 4.1, 1.4); leg III 11.8 (3.7, 1.5, 2.8, 2.7, 1.1); leg IV 12.3 (3.8, 1.4, 3.1, 2.9, 1.1). Leg formula: II-I-IV-III. Spination: palp: 130, 000, 0010, 1003 (bristles present on patella and tibia); Femur I 2(1)23, II III 323, IV 320(1); Patella I IV 000; Tibia I 1024, II 2024, III 2023, IV 2003; Metatarsus I III 2024, IV 3037(8). Metatarsus IV with ventral distal spine in sparse distal scopula, metatarsi I III without such a spine. Cheliceral furrow with 2 anterior and 4 posterior teeth; with 4 long bristles at posterior base of fang. Palpal claw with 8 9 teeth. Copulatory organ: As in diagnosis. Epigynal field as long as wide in its posterior part, bellshaped, with a number of small muscle sigilla marginally, without slit sense organs (Fig. 8). Copulatory ducts well developed, with smooth surface and wide lumen. Mem branous spermathecae elongated, wrinkled and asymmetrically displaced, leading to narrow posterior coils where fertilisation ducts start (Fig. 9); shimmer through cuticle in ventral view of uncleared epigyne (Fig. 8). Fertilisation ducts narrow with narrow lumen and smooth surface, their anterior part hidden in dorsal view by copulatory ducts (Fig. 9). Colouration in ethanol: As in male, but dorsal prosoma with patches fused with darker eye region to bands, excluding the foveal anterior patch and lateral patches. Chelicerae

6 206 AFRICAN INVERTEBRATES, vol. 55 (2), 2014 brown, dark brown distally (Fig. 14). Gnathocoxae and labium darker brown proximally (Fig. 15). Variation: : PL , OL Spination: Femur IV 321; Tibia IV Number of bristles at fang base: 3 7. Chelicerae with 3 posterior teeth, close together, barely fused. Membranous parts of bulb, conductor (tip) and RTA may vary in shape and according to dehydration status. The shape and arrangement of the two tegular apophyses vary slightly. Some specimens are generally lighter, i.e. with less pigmentation (e.g., PJ 3419, 3420): patches or spots in front of spinnerets or patterning on lateral opisthosoma may be absent. : PL , OL Spination: Femur IV 321 or 210, Tibia III 2022 or 2024, IV 2004 or Number of bristles at fang base: 3 6. Chelicerae with 3 posterior teeth, situated close together (Fig. 11); basal posterior tooth can be much smaller than other posterior teeth or of similar size. Some epigynes with mating plug. Dorsal prosoma has patches fused to an almost circular pattern and additional, separated lateral patches (Fig. 14). Type material examined: Holotype : (PJ 3417): Tanzania: Kibonoto, Zone des cultures, October, Sjöstedt leg. 1905/1906, (NHRM: with right palp; MHNG: left palp); examined. Note: The type locality was noted as Kibonoto. Today it is spelled Kibongoto and is located 2.7 km ENE of Sanya Juu. Data received from Google Earth: 03 10'42.91"S 37 05'35.23"E, 1360 m. Other material examined (11, 1 subadult, 14 ): SOUTH AFRICA: Kwazulu-Natal: Tembe Elephant Park: 1 (PJ 3418), 'S 'E, 100 m, sand forest, vegetation, Charles Haddad leg. 2004, DK 160, SD 42 (SMF). 1 subadult (PJ 3422) with bulbal structures developed under cuticle, same data as previous, DK 159, SD 292 (SMF). 1 (PJ 3423), same data as previous, DK 157, SD 45 (NMBA). 1 (PJ 3424), same data as previous, DK 158 (SMF). 1 (PJ 3436), same data as previous, DK 162, SD 44 (SMF). 1 (PJ 3487), same data as previous, DK 154, SD 299 (SMF). 1 (PJ 3432), same data as previous, DK 291, SD 318 (SMF). 1 (PJ 3435), same data as previous, DK 161, SD 41 (SMF). 1 (PJ 3486), same data as previous, DK 155 (SMF). Ndumo Game Reserve (post-excursion AFRAS 2005): 1 (PJ 3419), 'S 'E, 48 m, savannah, behind bark of Acacia xanthophloea (locality, Shokwe Pan ), Charles Haddad leg. 06.ii.2005, DK 287, SD 315 (SMF). 1 (PJ 3421), same data as previous but Dirk Kunz leg. 06.ii.2005, SD 314, DK 283 (RMCA). 1 (PJ 3433) with egg sac incl. 95 (prae)larvae, same data as previous but Dirk Kunz leg. 06.ii.2005, DK 285 (SMF). 1 (PJ 3420), 'S 'E, 35 m, savannah, behind bark of A. xanthophloea (locality, Shokwe Pan ), Dirk Kunz leg. 06.ii.2005, SD 313, DK 282 (PPRI). 1 (PJ 3426), same data as previous but Dirk Kunz leg. 09.ii.2005, DK 277, SD 312 (SMF). 1 (PJ 3425), 'S 'E, 28 m, savannah, behind bark of A. xanthophloea (locality, Shokwe Pan ), Dirk Kunz leg. 09.ii.2005, DK 308 (SMF). 1 (PJ 3427), 'S 'E, 35 m, savannah (locality Banzi Pan ), Dirk Kunz leg. 08.ii.2005, DK 277 (PPRI). 1 (PJ 3434), 'S 'E, 14 m (locality Banzi Pan ), Charles Haddad leg. 08.ii.2005, DK 296 (SMF). 1 (PJ 3428), 'S 'E, 29 m, savannah, behind bark of A. xanthophloea (locality Nyamiti Pan ), Charles Haddad, Milan Rezac, Franticek Stahlavsky, Benson Muramba, Dirk Kunz leg. 06.ii.2005, DK 271 (RMCA). 1 (PJ 3485), same data as previous but 'S 'E, 93 m, leg. 05.ii.2005, DK 265 (SMF). 1 (PJ 3429), 'S 'E, 40 m, savannah, under stone (locality Crocodile Farm ), Milan Rezac leg. 10.ii.2005, DK 322 (NMBA). 1 (PJ 3437), same data as previous but Charles Haddad leg. 10.ii.2005, DK 310 (SMF). 1 (PJ 3430), 'S 'E, 17 m, savannah, under stone (locality Ezikebheni ), Charles Haddad & Dirk Kunz leg. 07.ii.2005, DK 291, SD 318 (SMF). 1 (PJ 3431), 'S 'E, 16 m, on A. xanthophloea (locality Hotwe Pan ), Charles Haddad leg. 10.ii.2005, DK 312 (SMF). 1 Ophathe Game Reserve, 30.ix. 04.x.2008, pit traps, C. Haddad, S. Oliver & C. Hanekom leg. (PPRI, NCA 2008/4247). Gauteng: Pretoria, 1, 10.x.1997, in garden, B. Fourie leg. (PPRI, NCA 97/132). Botswana: 1, Okavango Delta Shakawe Fishing Camp, 29.vi.2006, C. Haddad leg. (PPRI, NCA 2007/984). Distribution: Known from the type locality in Tanzania (Kibonoto, Mount Kilimanjaro), from South Africa (northern KwaZulu-Natal: Ndumo Game Reserve, Tembe Elephant Park, Ophathe Game Reserve; Gauteng: Pretoria) and Botswana (Okavango Delta) (Fig. 16). Natural History: Olios sjostedti lives in forests and savannahs from sea level (northeastern South Africa) to 1400 m (Tanzania). Spiders have been recorded behind tree bark, in

7 Olios sjostedti from South Africa and Botswana 207 Fig. 16. Records of Olios sjostedti Lessert, 1921 in Africa: triangle type locality; Circles new records given in the present paper. vegetation and under stones. All of the males and females from South Africa were found in February, except for two males in October; in Botswana, one male was collected at the end of June. An egg sac of one female contained 95 praelarvae and larvae. Two females had an ecto-parasitic insect larva attached to their anterior opisthosoma, possibly Hymenoptera: Ichneumonidae (Fig. 14: arrow). Relationships: The male embolus of O. sjostedti is unique and completely different from all other embolus types within the genus. The simple structure might represent a secondary simplification, since other bulbal structures like the two tegular apophyses and the RTA seem to represent rather derived (complex) character states. A similar bauplan of the RTA with a strongly sclerotised dorsal part and a membranous ventral part as well as the two tegular apophyses are known for Olios claviger (Pocock, 1901) (Jäger & Kunz 2005: figs ), O. correvoni Lesert, 1921, O. darlingi (Pocock, 1901), O. faesi Lessert, 1933, and O. kassenjicola Strand, 1906, all of which exhibit an embolus with a characteristic distal apophysis and coil. Especially from the complex structure of the ventral RTA in combination with the particular shape of the dorsal RTA, a close relationship between O. sjostedti and the species listed above cannot be excluded. Females of O. sjostedti lack the distinct median slit that is usually widened to a V -shaped structure anteriorly, characters present in females of the abovementioned species (cf. Jäger & Kunz 2005: fig. 336). However, internal structures in O. sjostedti females follow the same bauplan as in other Olios species: from the medially situated copulatory orifices, ducts lead to membranous spermathecae and after one or more coils, back to the posteriorly situated fertilisation ducts. Thus, this species cannot be placed unambiguously in one particular species-group within Olios.

8 208 AFRICAN INVERTEBRATES, vol. 55 (2), 2014 ACKNOWLEDGEMENTS Dirk Kunz and Charles Haddad collected most of the material used in this study, Ansie Dippenaar- Schoeman sent data from the PPRI collection, and Charles Haddad commented on an earlier version of the manuscript. Peter Schwendinger (MHNG) loaned type material and Torbjorn Kronestedt supported my studies as curator in NRHM. The European Union-funded project SYNTHESYS provided financial support for a stay in Stock holm at NHRM. One referee made helpful comments and Burgert Muller handled the manuscript in the review process. Thanks to all of these persons and institutions for their generous support. REFERENCES Bonnet, P Bibliographia araneorum 2 (4). Toulouse: Douladoure, pp ICZN [International Commission on Zoological Nomenclature] International code of zoological nomenclature. Fourth edition. London: International Trust for Zoological Nomenclature. Jäger, P. & Kunz, D An illustrated key to genera of African huntsman spiders (Arachnida, Araneae, Sparassidae). Senckenbergiana Biologica 85 (2): Lessert, R. de Araignées du Kilimandjaro et du Merou (suite). IV. Clubionidae. Revue Suisse de Zoologie 28: Platnick, N.I The world spider catalog. New York: American Museum of Natural History. ( web.archive.org/web/ / accessed 19/02/2013) The world spider catalog, Version 15. New York: American Museum of Natural History. ( accessed 22/07/2014). DOI: /db.iz Roewer, C. F Katalog der Araneae von 1758 bis 1940, bzw Bruxelles: Institut Royal des Sciences Naturelles de Belgique, 2: