3D Cell Culture Quick Reference Ordering Guide

Transcription

1 3D Cell Culture Quick Reference Ordering Guide Permeable Supports Qty/ Qty/ Transwell inserts in 6-well plates, 24 mm, 0.4 µm pore PC membrane, sterile Transwell inserts in 6-well plates, 24 mm, 3.0 µm pore PC membrane, sterile Transwell inserts in 6-well plates, 24 mm, 8.0 µm pore PC membrane, sterile Transwell inserts in 12-well plates, 12 mm, 0.4 µm pore PC membrane, sterile Transwell inserts in 12-well plates, 12 mm, 3.0 µm pore PC membrane, sterile Transwell inserts in 24-well plates, 6.5 mm, 0.4 µm pore PC membrane, sterile Transwell inserts in 24-well plates, 6.5 mm, 3.0 µm pore PC membrane, sterile Transwell inserts in 24-well plates, 6.5 mm, 5.0 µm pore PC membrane, sterile Transwell inserts in 24-well plates, 6.5 mm, 8.0 µm pore PC membrane, sterile Transwell inserts in 100 mm dish, 75 mm, 0.4 µm pore PC membrane, sterile Transwell inserts in 100 mm dish, 75 mm, 3.0 µm pore PC membrane, sterile Transwell clear inserts in 6-well plates, 24 mm, 0.4 µm pore PET membrane, sterile Transwell clear inserts in 6-well plates, 24 mm, 3.0 µm pore PET membrane, sterile Transwell clear inserts in 12-well plates, 12 mm, 0.4 µm pore PET membrane, sterile Transwell clear inserts in 12-well plates, 12 mm, 3.0 µm pore PET membrane, sterile Transwell clear inserts in 24-well plates, 6.5 mm, 0.4 µm pore PET membrane, sterile Transwell clear inserts in 24-well plates, 6.5 mm, 3.0 µm pore PET membrane, sterile Transwell clear inserts in 24-well plates, 6.5 mm, 8.0 µm pore PET membrane, sterile Transwell-COL Collagen-coated inserts and 6-well plates, 24 mm, with 0.4 µm pore PTFE membrane, sterile Transwell-COL Collagen-coated inserts and 6-well plates, 24 mm, with 3.0 µm pore PTFE membrane, sterile Transwell-COL Collagen-coated inserts and 12-well plates, 12 mm, 0.4 µm pore PTFE membrane, sterile Transwell-COL Collagen-coated inserts and 12-well plates, 12 mm, 3.0 µm pore PTFE membrane, sterile Transwell-COL Collagen-coated inserts and 24-well plates, 6.5 mm, 0.4 µm pore PTFE membrane, sterile Transwell-COL Collagen-coated inserts and 24-well plates, 6.5 mm, 3.0 µm pore PTFE membrane, sterile Netwell inserts in 6-well plates, 24 mm with 74 µm mesh size with PET membrane, sterile Netwell inserts in 6-well plates, 24 mm with 440 µm mesh size with PET membrane, sterile Netwell inserts in 12-well plates, 15 mm with 74 µm mesh size with PET membrane, sterile Netwell inserts in 12-well plates, 15 mm with 440 µm mesh size with PET membrane, sterile Netwell reagent tray, black, nonsterile Netwell reagent tray, white, nonsterile Netwell 6-well carrier kit for 24 mm inserts, nonsterile Netwell 12-well carrier kit for 15 mm inserts, nonsterile Snapwell PC inserts in 6-well plates, 12 mm with 0.4 µm pore membrane, sterile Snapwell clear PET inserts in 6-well plates, 12 mm with 0.4 µm pore membrane, sterile HTS Transwell-96 well insert, receiver plate and lid, individual, with 8.0 µm pore PET membrane, sterile HTS Transwell-24 well inserts, bulk, with 0.4 µm pore PET membrane, sterile HTS Transwell-24 well inserts, individual, with 0.4 µm pore PET membrane, sterile HTS Transwell-96 system, reservoir, and receiver plates with 2 lids, PET with 1.0 µm pore PET membrane, sterile HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PC with 0.4 µm pore, sterile HTS Transwell-96 receiver plate with lid, standard TC-treated, sterile HTS Transwell-96 reservoir plate media stabilizer and lid, not treated, sterile HTS Transwell-96 well inserts, receiver plate and lid, bulk, PET, with 8.0 µm pore, sterile HTS Transwell-96 well inserts, receiver plate and lid, individual, PC, with 3.0 µm pore, sterile HTS Transwell-96 well inserts, receiver plate and lid, bulk, PC, with 3.0 µm pore, sterile HTS Transwell-96 well inserts, receiver plate and lid, bulk, PC, with 5.0 µm pore, sterile HTS Transwell-96 well inserts, receiver plate and lid, individual, PC, with 5.0 µm pore, sterile HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PC, with 0.4 µm pore, sterile HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PET, sterile HTS Transwell nontreated reservoir plate, clear, sterile HTS Transwell-24, individual, PC, with 0.4 µm pore, sterile HTS Transwell-24, bulk, PC, with 0.4 µm pore, sterile HTS Transwell-24, individual, PC, with 3.0 µm pore, sterile HTS Transwell-24, bulk, PC, with 3.0 µm pore, sterile 12 12

2 Permeable Supports (Continued) Qty/ Qty/ HTS Transwell -96 black receiver plate with lid, standard TC-treated, sterile HTS Transwell-96 white receiver plate, and lid, standard TC-treated, sterile HTS Transwell-96 system reservoir and receiver plates with 2 lids, PET with 0.4 µm pore, sterile Falcon inserts for 6-well plates with 0.4 μm pore transparent PET membrane, sterile Falcon inserts for 6-well plates with 1.0 µm transparent PET membrane, sterile Falcon inserts for 6-well plates with 3.0 µm transparent PET membrane, sterile Falcon inserts for 6-well plates with 8.0 µm transparent PET membrane, sterile Falcon inserts for 12-well plates with 0.4 µm transparent PET membrane, sterile Falcon inserts for 12-well plates with 1.0 µm transparent PET membrane, sterile Falcon inserts for 12-well plates with 3.0 µm transparent PET membrane, sterile Falcon inserts for 12-well plates with 8.0 µm transparent PET membrane Falcon inserts for 24-well plates with 0.4 µm transparent PET membrane, sterile Falcon inserts for 24-well plates with 1.0 µm transparent PET membrane, sterile Falcon inserts for 24-well plates with 3.0 µm transparent PET membrane, sterile Falcon inserts for 24-well plates with 8.0 µm transparent PET membrane, sterile Falcon inserts for 6-well plates with 0.4 µm translucent high density PET membrane, sterile Falcon inserts for 6-well plates with 3.0 µm translucent high density PET membrane, sterile Falcon inserts for 12-well plates with 0.4 µm translucent high density PET membrane, sterile Falcon inserts for 12-well plates with 3.0 µm translucent high density PET membrane, sterile Falcon inserts for 24-well plates with 0.4 µm translucent high density PET membrane, sterile Falcon inserts for 24-well plates with 3.0 µm translucent high density PET membrane, sterile Falcon inserts for 24-well plates with 0.4 µm translucent high density PET membrane, sterile Falcon 6-well TC-treated PS cell culture insert companion plate, with lid, sterile Falcon 12-well TC-treated PS cell culture insert companion plate, with lid, sterile Falcon 24-well TC-treated PS cell culture insert companion plate, with lid, sterile 1 50 BD Falcon 96-well insert system with 1.0 μm pore PET membrane, with feeder tray and lid 1 1 BD Falcon 96-well insert system with 1.0 μm pore PET membrane, with feeder tray and lid 5 5 BD FluoroBlok 24 multiwell insert system with 3.0 μm pore PET membrane, sterile 1 1 BD FluoroBlok 24 multiwell insert system with 3.0 μm pore PET membrane, sterile FluoroBlok inserts for 24-well plates, with 3.0 μm pore PET membrane, sterile FluoroBlok inserts for 24-well plates, with 8.0 μm pore PET membrane, sterile 8 48 BD FluoroBlok 24 multiwell insert system with 8.0 μm pore PET membrane, sterile 1 1 BD FluoroBlok 24 multiwell insert system with 8.0 μm pore PET membrane, sterile 5 5 BD FluoroBlok 96-well multiwell insert system with 3.0 μm pore PET membrane, sterile 1 1 BD FluoroBlok 96-well multiwell insert system with 3.0 μm pore PET membrane, sterile 5 5 BD FluoroBlok 96-well multiwell insert system with 8.0 μm pore PET membrane, sterile 1 1 BD FluoroBlok 96-well multiwell insert system with 8.0 μm pore PET membrane, sterile Falcon 24-well multiwell insert system with 1.0 μm pore high density PET membrane Falcon 24-well multiwell insert system with 1.0 μm pore PET membrane, with feeder tray and lid Falcon HTS 24-well multiwell insert system with 3.0 μm pore high density PET membrane, sterile 1 1 BD Falcon HTS 24-well multiwell insert system with 3.0 μm pore PET membrane, with 24-well plate and lid, sterile Falcon HTS 24-well multiwell insert system with 8.0 μm pore PET membrane, with 24-well plate and lid, sterile 1 1 BD Falcon HTS 24-well multiwell insert system with 8.0 μm pore PET membrane, with 24-well plate and lid, sterile 5 5 BD Falcon 24-well PS feeder tray, with lid, sterile 5 5 BD Falcon 96-well PS feeder tray, with lid, sterile 5 5 BD Falcon 96 square well angled-bottom microplate and lid, not treated, sterile 5 5 BD Falcon 96 square well PS flat-bottom microplate, with lid, sterile Falcon 96-well insert system with 1.0 µm PET membrane, with square well angled-bottom microplate 5 5 and lid, sterile Vialed Surfaces Qty/ Qty/ Matrigel basement membrane matrix (5 x 5 ml) Matrigel basement membrane (10 x 10 ml) Matrigel basement membrane matrix, LDEV-free, 5 ml Matrigel basement membrane matrix, LDEV-free, 10 ml Matrigel basement membrane matrix, LDEV-free, 50 ml Matrigel basement membrane matrix, phenol red-free, LDEV-free, 10 ml Matrigel hesc-qualified matrix (5 x 5 ml) 1 5 BD Matrigel hesc-qualified matrix, LDEV-free, 5 ml Matrigel hesc-qualified matrix, LDEV-free (50 ml) Matrigel Growth Factor Reduced (GFR) basement membrane matrix, LDEV-free, 5 ml Matrigel GFR basement membrane matrix, LDEV-free, 10 ml 1 1 2

3 Vialed Surfaces (Continued) Qty/ Qty/ Matrigel GFR basement membrane matrix, phenol red-free, LDEV-free, 10 ml Matrigel GFR basement membrane, phenol red-free, LDEV-free (5 x 10 ml) Matrigel GFR basement membrane, phenol red-free, LDEV-free (10 x 10 ml) Matrigel GFR basement membrane, LDEV-free (5 x 10 ml) Matrigel GFR basement membrane, LDEV-free (10 x 10 ml) Matrigel basement membrane matrix high concentration (HC), LDEV-free, 10 ml Matrigel matrix HC, phenol red-free, LDEV-free, 10 ml Matrigel matrix HC, GFR, LDEV-free, 10 ml * Collagen I, bovine, 30 mg Collagen I, rat tail, 100 mg Collagen I, rat tail, 1g (10 x 100 mg) Collagen I, high concentration, rat tail, 100 mg Ultrapure Laminin, mouse, 1 mg Laminin, mouse, 1 mg Laminin/Entactin high concentration, 10.5 mg Extracellular matrix, human, 1 mg PuraMatrix Peptide Hydrogel, 5 ml 1 1 *Available only in the United States. Pre-coated Cultureware BioCoat Matrigel matrix 6-well clear flat-bottom TC-treated multiwell plate, with lid BioCoat Matrigel matrix 6-well clear flat-bottom TC-treated multiwell plate for hepatocytes, with lid BioCoat Matrigel matrix 12-well clear flat-bottom TC-treated multiwell plate, with lid BioCoat Matrigel matrix 24-well clear flat-bottom multiwell plate, with lid BioCoat Matrigel matrix 48-well clear flat-bottom multiwell plate, with lid BioCoat Matrigel matrix 35 mm culture dishes 4 8 Kits Endothelial cell culture medium kit 1 Kit BioCoat endothelial cell growth environment kit 1 Kit Hepatocyte culture media kit, 500 ml 1 Kit Intestinal epithelium differentiation environment kit 1 Kit Intestinal differentiation media pack kit 1 Kit Ultra-Low Attachment Surface Cultureware Costar 6-well clear flat bottom Ultra-Low Attachment (ULA) surface multiple well plates, individually wrapped, sterile Costar 24-well clear flat bottom ULA surface multiple well plates, individually wrapped, sterile well clear flat bottom ULA surface microplate, individually wrapped, with lid, sterile well black/clear round-bottom ULA surface, spheroid microplate, 1 5 bulk packed, with lid, sterile well black/clear round-bottom ULA surface spheroid microplate, individually packed, with lid, sterile well clear round bottom ULA surface microplate, individually wrapped, with lid, sterile well black round bottom Polystyrene ULA surface microplate, with lid, sterile well black/clear round-bottom ULA surface spheroid microplate, with lid, sterile well black/clear round-bottom ULA surface spheroid microplate, with lid, sterile BC 384-well black/clear round-bottom ULA surface spheroid microplate, bulk, with lid, sterile, with generic barcodes mm ULA surface culture dish mm ULA surface culture dish cm² rectangular canted neck ULA surface cell culture flask with vent cap cm² U-shaped canted neck ULA surface cell culture flask with vent cap 4 24 CoolCell Systems CoolCell, purple CoolCell LX, purple cell freezing container, for 12 x 1 ml or 2 ml cryogenic vials CoolCell LX, green cell freezing container, for 12 x 1 ml or 2 ml cryogenic vials CoolCell LX, orange cell freezing container, for 12 x 1 ml or 2 ml cryogenic vials CoolCell LX, pink cell freezing container, for 12 x 1 ml or 2 ml cryogenic vials CoolCell 5 ml LX, purple cell freezing container, for 12 x 3.5 ml to 5 ml cryogenic vials CoolCell FTS30, purple freezing container, for 30 x 1 ml or 2 ml cryogenic vials CoolCell FTS30, orange freezing container, for 30 x 1 ml or 2 ml cryogenic vials CoolCell FTS30, green freezing container, for 30 x 1 ml or 2 ml cryogenic vials CoolCell FTS30, pink freezing container, for 30 x 1 ml or 2 ml cryogenic vials CoolCell SV2 freezing container, for 12 x 2 ml serum vials CoolCell SV10 freezing container, for 6 x 10 ml serum vials 1 3

4 CoolCell Accessories Filler vials for CoolCell freezing container to use when freezing less than a full batch of vials, 2 ml size, 6 fits CoolCell, CoolCell LX, and CoolCell FTS30 containers Filler vials for CoolCell freezing container to use when freezing less than a full batch of vials, 5 ml size, 6 fits CoolCell 5 ml LX container Removable cryogenic vial module for CoolCell FTS Cryogenic vial grippers, assorted colors 5 CoolBox CoolBox XT, purple cooling and freezing workstation, single capacity CoolBox XT, green cooling and freezing workstation, single capacity CoolBox XT, orange cooling and freezing workstation, single capacity CoolBox XT, pink cooling and freezing workstation, single capacity CoolBox 2XT, purple cooling and freezing workstation, double capacity CoolBox 2XT, green cooling and freezing workstation, double capacity CoolBox 2XT, orange cooling and freezing workstation, double capacity CoolBox 2XT, pink cooling and freezing workstation, double capacity XT Starter complete, low profile, open platform ice-free cooler XT Starter holder only open platform ice free cooler 1 CoolBox Accessories XT Cooling core for CoolBox XT workstations, for 0.5 C to 4 C XT Freezing core for CoolBox XT workstations, for below 0 C Extension collar for CoolBox XT, purple Extension collar for CoolBox XT, green Extension collar for CoolBox XT, orange Extension collar for CoolBox XT, pink Extension collar for CoolBox 2XT, purple Extension collar for CoolBox 2XT, green Extension collar for CoolBox 2XT, orange Extension collar for CoolBox 2XT, pink C to 8 C adhesive strip LCD temperature display Elastic sleeves for CoolRack modules 4 CoolRack CoolRack M6, gray, holds 6 x 1.5 or 2 ml microcentrifuge tubes CoolRack M6, green, holds 6 x 1.5 or 2 ml microcentrifuge tubes CoolRack M6, orange, holds 6 x 1.5 or 2 ml microcentrifuge tubes CoolRack M15, gray, holds 15 x 1.5 or 2 ml microcentrifuge tubes CoolRack M15, green, holds 15 x 1.5 or 2 ml microcentrifuge tubes CoolRack M15, orange, holds 15 x 1.5 or 2 ml microcentrifuge tubes CoolRack XT M24, holds 24 x 1.5 or 2 ml microcentrifuge tubes CoolRack M30, gray, holds 30 x 1.5 or 2 ml microcentrifuge tubes CoolRack M30, green, holds 30 x 1.5 or 2 ml microcentrifuge tubes CoolRack M30, orange, holds 30 x 1.5 or 2 ml microcentrifuge tubes CoolRack M90, gray, holds 90 x 1.5 or 2 ml microcentrifuge tubes CoolRack M96, gray, holds 96 x 1.5 or 2 ml microcentrifuge tubes with A-H and 1-12 row and column indexing CoolRack 500 µl M30-PF, gray, holds 30 x 500 µl microcentrifuge tubes, tapered wells for conical tubes CoolRack M15-PF, gray, holds 15 x 1.5 ml microcentrifuge tubes, tapered wells for conical tubes CoolRack M30-PF, gray, holds 30 x 1.5 ml microcentrifuge tubes, tapered wells for conical tubes CoolRack CF15, holds 15 cryogenic or FACS tubes CoolRack XT CFT24, holds 24 cryogenic or FACS tubes, with gripping wells for one-hand vial opening/closing CoolRack CF45, holds 45 cryogenic or FACS tubes CoolRack CFT30, holds 30 cryogenic or FACS tubes, with gripping wells for one-hand vial opening/closing CoolRack XT PCR96 for PCR 12-strip tubes CoolRack XT-M-PCR, holds 12 x 1.5 or 2 ml microcentrifuge tubes and PCR 6-strip tubes CoolRack XT PCR384 for 384-well microplate CoolRack 9 6x 0.5 ml, for 96 x 0.5 ml 2D tubes CoolRack 96 x 1 ml, for 96 x 1.4 ml 2D tubes 1 4

5 CoolRack (Continued) CoolRack SV2 holds 12 x 5 ml injectable ampules CoolRack SV10 holds 12 x 10 ml injectable ampules CoolRack XT 5 ml holds 12 x 5 ml centrifuge tubes CoolRack 15 ml, holds 9 x 15 ml conical centrifuge tube CoolRack 50 ml, holds 4 x 50 ml conical centrifuge tube CoolRack 250 ml, holds 1 x 250 ml conical centrifuge tube CoolRack 250 ml, holds 1 x 250 ml Easy Grip storage bottle CoolRack VS13, holds 9 x 13 x 75 mm blood tubes CoolRack V16, holds 9 x 16 x 100 mm blood tubes CoolRack V13, holds 9 x 13 x 100 mm blood tubes CoolRack L, lightweight insulated module for 12 x 15 ml centrifuge tubes CoolRack LV, lightweight, insulated module for 12 x 13 mm or 16 mm diameter tubes CoolSink XT96F, 96-well flat-bottom microplate module CoolSink XT 96U, 96-well U-bottom microplate module CoolSink LX55, 55 ml reagent reservoir module ThermalTray SLP, low profile for liquid nitrogen ThermalTray LP, medium-profile for ice ThermalTray HP, high-profile for water bath 1 Media CV 500 ml DMEM [+] 4.5 g/l glucose, L-glutamine, sodium pyruvate CV 500 ml DMEM [+] 4.5 g/l glucose, L-glutamine [-] sodium pyruvate CV 500 ml DMEM [+] 4.5 g/l glucose, sodium pyruvate [-] L-glutamine CV 500 ml DMEM/F12 50:50 mix [-] L-glutamine CV 500 ml DMEM/Ham s F-12 50/50 mix [+] L-glutamine, 15 mm HEPES CV 500 ml DMEM/Ham s F-12 50/50 mix [+] L-glutamine [-] phenol red CV 500 ml Iscoves Modification of DMEM [+] 25 mm HEPES, [-] ß-thioglycerol, ß-mercaptoethanol, 6 L-glutamine CV 500 ml Iscoves Modification of DMEM [+] L-glutamine, 25 mm HEPES [-] ß-thioglycerol, 6 ß-mercaptoethanol CV 500 ml Ham s F-12 Medium [+] L-glutamine CV 500 ml Medium 199 (Mod.) [+] Earle s salts, L-glutamine CV 500 ml MEM alpha medium [+] Earle s salts, ribonucleosides, deoxyribonucleosides, L-glutamine CV 500 ml MEM alpha medium [+] Earle s salts [-] ribonucleosides, deoxyribonucleosides, L-glutamine CV 500 ml MEM [+] Earle s salts, L-glutamine CV 500 ml MEM [+] 1.5 g/l sodium bicarbonate, NEAA, L-glutamine, sodium pyruvate CV 500 ml MEM [+] Earle s salts [-] L-glutamine CV 500 ml MEM [+] Earle s salts [-] L-glutamine, phenol red CV 500 ml RPMI 1640 [+] L-glutamine CV 500 ml RPMI 1640 [-] L-glutamine CV 500 ml RPMI 1640 [-] L-glutamine, phenol red CV 500 ml RPMI 1640 [+] L-glutamine, 25 mm HEPES CV 500 ml RPMI 1640 [+] 25 mm HEPES [-] L-glutamine 6 Reagents Cat. No. Cat. No. Description Qty/Pk Qty/Cs CQC Dimethyl Sulfoxide, 250 ml Cell Recovery Solution, 100 ml Calcein AM fluorescent dye, 500 µg (10 x 50 µg) Calcein AM fluorescent dye, 1 mg DiIC12(3) fluorescent dye, 100 mg Dispase, 100 ml 1 1 Serum and Protein-free Media CV SF medium, serum-free/low-protein medium, 1X with L-glutamine CV SF medium, serum-free/low-protein medium, 1X with L-glutamine, 1 g/l BSA without phenol red CV PF medium, serum-free/low-protein medium, 1X without L-glutamine 6 5

6 Supplements CI glutagro supplement CI Trace Elements A CI Trace Elements B CI Trace Elements C CI MEM Amino acids CI MEM Nonessential amino acids CR Insulin-Transferrin-Selenium (ITS) CV Fetal Bovine Serum (FBS), regular S Nerve Growth Factor (NGF), mouse natural (10 µg) Epidermal Growth Factor (EGF), human recombinant, 1 mg (10 x 10 µg) Vascular Endothelial Growth Factor (VEGF), human recombinant, 10 µg Endothelial Cell Growth Supplement (ECGS), 100 mg 1 Buffers CV Phosphate-Buffered Saline (PBS), 1X without calcium and magnesium CV Hanks Balanced Salt Solution (HBSS), 1X with calcium and magnesium CV HBSS, 1X with calcium and magnesium without phenol red CV HBSS, 1X without calcium and magnesium CV HBSS, 1X without calcium, magnesium, and phenol red CV Dulbecco s Phosphate-Buffered Saline (DPBS), 1X with calcium and magnesium CV DPBS, 1X without calcium and magnesium 6 Warranty/Disclaimer: Unless otherwise specified, all products are for research use only. Not intended for use in diagnostic or therapeutic procedures. Not for use in humans. Life Sciences makes no claims regarding the performance of these products for clinical or diagnostic applications. For additional product or technical information, visit vwr.com/corning, call , or contact your representative. For a listing of trademarks, visit All other trademarks are the property of their respective owners Lit. No WREV 2018 Incorporated. All rights reserved. 5/18 CLS-DIV-008