Breaking the depth dependency of phototherapy with Cerenkov radiation and low-radianceresponsive
|
|
- Adele Garrett
- 5 years ago
- Views:
Transcription
1 SUPPLEMENTARY INFORMATION DOI: /NNANO Breaking the depth dependency of phototherapy with Cerenkov radiation and low-radianceresponsive nanophotosensitizers Nalinikanth Kotagiri, Gail P. Sudlow, Walter J. Akers, Samuel Achilefu* NATURE NANOTECHNOLOGY 1
2 Supplementary Discussion Interaction of diverse materials with CR In addition to TiO 2, studies have shown that some materials which are capable of absorbing energy in the nm wavelength range can harvest CR for imaging applications 1-4. For example, quantum dots (QDs) are known to generate singlet oxygen radicals upon excitation with light, albeit with a low quantum yield 5. In conjunction with CR, QDs have appreciable fluorescence quantum yield for in vitro and in vivo imaging applications. Thus, it is entirely plausible to use QDs as nanophotosentizers (NPS) for CRIT. Enhancement of TiO 2 CRIT with Tc Although TiO 2 can serve as NPS under both aerobic and hypoxic conditions, intrinsic antioxidants in cells such as superoxide dismutase (SOD) are capable of inhibiting the oxidative damage caused by radicals such as of superoxide species. This could reduce the contribution of superoxide to therapy 6,7. Thus complementing TiO 2 cytotoxicity with other sources of free radicals could further enhance the effectiveness of CRIT. To accomplish this goal, we incorporated titanocene (Tc), a photoinitiator, into the NPS. Physical characterization of TiO 2 -Tf A strong signature of carbon in the Energy-dispersive X-ray spectroscopy (EDX) spectrum of TiO 2 -Tf compared to TiO 2 alone (Supplementary Fig. 1a) confirmed the presence of Tf on the surface of TiO 2 (Supplementary Fig. 1b). Electron diffraction analysis confirmed that the crystal lattice structure of anatase TiO 2 remained unchanged as a result of processing with Tf (Supplementary Fig. 1c,d). Phase transformation between anatase and rutile forms of TiO 2 2
3 typically occur at temperatures exceeding 700 C 8. Due to the relatively mild nature of the processing used to generate TiO 2 -Tf adducts, the photocatalytic properties of anatase TiO 2 employed in this study was thus maintained. The ring measurements are as follows: TiO /nm/2 = nm = 3.51 A 8.4 1/nm/2 = nm = 2.38 A 10.51/nm/2 = nm = 1.89 A 11.81/nm/2 = nm = 1.69 A TiO 2 -Tf /nm/2 = nm = 3.51 A 8.4 1/nm/2 = nm = 2.38 A /nm/2 = nm = 1.90 A /nm/2 = nm = 1.69 A Supplementary Fig. 1. Composition and phase characterization of TiO 2 -Tf. a, EDX spectra of unprocessed TiO 2 with the peaks labelled as Ti for titanium, O for oxygen and C for carbon. 3
4 b, EDX spectra of TiO 2 -Tf with a pronounced C peak suggesting presence of the protein Tf on the surface of TiO 2. c, Electron diffraction of unprocessed TiO 2 with ring measurements matching the crystal pattern of anatase form of TiO 2, from diffraction file: d, Electron diffraction pattern of TiO 2 -Tf with ring measurements and crystal structure identical to that of TiO 2. Serum stability of TiO 2 -Tf To determine the serum stability of the TiO 2 -Tf interaction, we incubated the nanoparticles in foetal bovine serum for 24 h. We found that the amount of bound AlexaTf to TiO 2 surface did not significantly change with time (Supplementary Fig. 2a). Further analysis of data showed that serum components such as albumin, using Alexa 680 labelled BSA, did not form appreciable protein corona on the TiO 2 -Tf surface relative to pristine TiO 2 (Supplementary Fig. 2b), confirming that Tf does not readily exchange with serum proteins. Supplementary Fig. 2. Serum stability of TiO 2 -Tf NPS. a, Comparison of fluorescence intensity between TiO 2 -AlexaTf NPS incubated in foetal bovine serum for 24 h and untreated samples (ns: not significant). b, Comparison of unlabelled TiO 2 -Tf and unprocessed TiO 2 incubated with Alexa 680 labelled albumin. Values are means ± s.e.m. (experiments for each group were run in triplicates). **P <
5 Optical properties of TiO 2 and Tc Coincidentally, the excitation energy for TiO 2 (Supplementary Fig. 3a) and Tc (Supplementary Fig. 3b) is in the UV spectrum, where CR quantum efficiency (Supplementary Fig. 3c) is the highest. Supplementary Fig. 3. Spectral characterization of TiO 2 and Tc. a, Absorption spectrum of TiO 2 in water. b, Absorption spectrum of Tc in water/dmso (95/5%). c, Emission spectrum of CR from 64 Cu. d, Fluorescence spectrum of TiO 2, excited at 275 nm. CPS, counts per second. e, In vitro luminescence studies carried out with 0.1 mci/100 l of 64 Cu, 0.1 mci/100 l 64 Cu admixed with 1 mg/ml TiO 2, and 0.1 mci/100 l of 99m Tc admixed with 1mg/ml TiO 2, in each 5
6 well. Images were captured in the GFP channel ( nm) in an IVIS Lumina XR multimodal imaging system (Caliper Life Sciences Inc.) using Living Image software. In vivo blocking study using TiO 2 -Tf labelled with Alexa 680 Supplementary Fig. 4. In vivo blocking of TiO 2 -Tf uptake by HT1080 tumours. a, Organ biodistribution of TiO 2 -AlexaTf. b, Organ biodistribution of TiO 2 -AlexaTf after administration of holo-tf to block Tf receptors. c, Comparison of biodistribution of TiO 2 -AlexaTf with and without blocking. Intrinsic toxicity of TiO 2, Tc, FDG, and 64 Cu 6
7 Our cytotoxicity analysis revealed that TiO 2 NPS and Tc did not induce apoptosis at 4 g/ml and 10 g/ml, respectively (Supplementary Fig. S5a). Similarly, viability in 64 Cu ( 0.5 mci (18.5 MBq)/0.1 ml) and FDG (1 mci (37 MBq)/0.1 ml) treated cells was >95% relative to untreated control (Supplementary Fig. S5b). Supplementary Fig. 5. Assessment of the intrinsic and stimulated cytotoxicity of the photoactive products using MTS cytotoxicity assay. a, Viability of cells treated with Tc-Tf, TiO 2 -Tf and TiO 2 -PEG. The control group was considered to be 100% viable. b, Viability of cells treated with 64 Cu and FDG. HT1080 cells were used and the values are means ± s.e.m. (experiments for each group were run in triplicates). Free radicals generated by TiO 2 Hydroxyl radicals, are highly reactive short lived species, non-diffusible across cell membranes, causing a highly pronounced local therapeutic effect (~ 20 nm) that typically culminates into necrosis 9,10. In contrast, superoxide radicals are more stable species that can travel across cell membranes with a long diffusion distance of ~320 nm and result in disruptions of lipid membranes such as plasma and mitochondrial membranes leading to apoptosis 31. 7
8 Supplementary Fig. 6. Confocal imaging of TiO 2 free radicals. Images of merged bright-field and fluorescence images comparing the degree of hydroxyl radical generation caused by 0.1, 0.25, 0.5, 1 mci/100 l of 64 Cu on tumour cells with 2.5 g/ml TiO 2 -PEG after 4 h. HPF dye was used to stain the cells. The green fluorescence from cells depicts increased hydroxyl radical generation. Highest fluorescence intensity was recorded from cells incubated with 0.25 mci/100 l of 64 Cu. Scale bar, 20 m. In vitro assessment of mitochondrial membrane potential as a result of CRIT To further understand the role of free radicals and the mechanism of cell death, we evaluated whether CRIT induces alterations in mitochondrial membrane potential. There was a significant decrease in mitochondrial membrane potential in cells treated with both TiO 2 and Tc coincubated with FDG, indicating damaged and leaky membranes. Typically, damage to mitochondrial membranes initiates the intrinsic signalling pathway for apoptosis, characterized by loss of membrane potential and a cascade of events involving caspases leading to nuclear fragmentation and cell death. 8
9 Supplementary Fig. 7. Loss of mitochondrial membrane potential due to CRIT. Mitochondrial membrane potential changes detected by Mitotracker Green dye as a result of CRIT. Values are means ± s.e.m. *P < 0.05, **P < Assessment of body weight and remission after achieving CRIT induced regression of tumours 9
10 Supplementary Fig. 8. Change in murine weights in untreated and treated groups of mice. TiO 2 - PEG and chelated 64 Cu were administered intratumourally and monitored over 4 months. Assessment of off-target toxicity in liver and kidneys 10
11 Supplementary Fig. 9. Histologic images of tissues. H&E stained liver and kidney sections before and after treatment did not show significant lesions in these organs, indicating the absence of systemic toxicity from CRIT. References 1. Dothager, R.S., Goiffon, R.J., Jackson, E., Harpstrite, S. & Piwnica-Worms, D. Cerenkov radiation energy transfer (CRET) imaging: a novel method for optical imaging of PET isotopes in biological systems. PLoS ONE 5, (2010). 2. Liu, H., et al. Radiation-luminescence-excited quantum dots for in vivo multiplexed optical imaging. Small 6, (2010). 3. Carpenter, C.M., et al. Radioluminescent nanophosphors enable multiplexed smallanimal imaging. Opt. Express 20, (2012). 4. Ran, C., Zhang, Z., Hooker, J. & Moore, A. In vivo photoactivation without "light": use of Cherenkov radiation to overcome the penetration limit of light. Mol. Imaging Biol. 14, (2012). 5. Bakalova, R., Ohba, H., Zhelev, Z., Ishikawa, M. & Baba, Y. Quantum dots as photosensitizers? Nat. Biotech. 22, (2004). 6. Kono, Y. & Fridovich, I. Superoxide radical inhibits catalase. J. Biol. Chem. 257, (1982). 7. Morel, D.W., Hessler, J.R. & Chisolm, G.M. Low density lipoprotein cytotoxicity induced by free radical peroxidation of lipid. J. Lipid Res. 24, (1983). 8. Li, W., Ni, C., Lin, H., Huang, C.P. & Shah, S.I. Size dependence of thermal stability of TiO2 nanoparticles. J. Appl.Phys. 96, (2004). 9. Apel, K. & Hirt, H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55, (2004). 10. Rozhkova, E.A., et al. A high-performance nanobio photocatalyst for targeted brain cancer therapy. Nano Lett. 9, (2009). 11
Electronic Supplementary Information
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 28 Electronic Supplementary Information Nano-Engineering Safer-By-Design Nanoparticle Based Moth-Eye
More informationCell membrane penetration and mitochondrial targeting by platinumdecorated
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2016 Cell membrane penetration and mitochondrial targeting by platinumdecorated ceria nanoparticles
More informationASSESSMENT OF CELLULAR OXYGEN GRADIENTS WITH A PANEL OF PHOSPHORESCENT OXYGEN-SENSITIVE PROBES
ASSESSMENT OF CELLULAR OXYGEN GRADIENTS WITH A PANEL OF PHOSPHORESCENT OXYGEN-SENSITIVE PROBES Ruslan I. Dmitriev, Alexander V. Zhdanov, Greg Jasionek, Dmitri B. Papkovsky Biochemistry Department, University
More informationSupporting Information
Supporting Information Toward High-Efficient Red Emissive Carbon Dots: Facile Preparation, Unique Properties, and Applications as Multifunctional Theranostic Agents Shan Sun,, Ling Zhang, Kai Jiang, Aiguo
More informationB16-F10 (Mus musculus skin melanoma), NCI-H460 (human non-small cell lung cancer
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Experimental Methods Cell culture B16-F10 (Mus musculus skin melanoma), NCI-H460 (human non-small
More informationSupporting information
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2014 Supporting information Seeing the Diabetes: Visual Detection of Glucose Based on the Intrinsic
More informationSHG Nanoprobes: Advancing harmonic imaging in biology. Periklis (Laki) Pantazis
SHG Nanoprobes: Advancing harmonic imaging in biology Periklis (Laki) Pantazis fluorescence microscopy in biomedical research: impact of fluorescent proteins 1960s and 1970s 1992 and 1994 1995 1996 wt-gfp
More informationSupporting Information
Supporting Information R6G/8-AQ co-functionalized Fe 3 O 4 @SiO 2 nanoparticles for fluorescence detection of trace Hg 2+ and Zn 2+ in aqueous solution Yao Gu 1,2, Guowen Meng 1,3*, Meiling Wang 1, Qing
More informationMaterial properties that control the cytotoxicity of ZnO nanoparticles
Material properties that control the cytotoxicity of ZnO nanoparticles Alex Punnoose, Jordan Chess, Catherine Anders, John Rassmussen, Denise Wingett, Kelsey Dodge and Katie Rainey, Boise State University,
More informationAgriTitan TM. Effective, Safe, & Smart. Harvest the Power of Light To Control Bacterial Plant Disease. EcoActive Surfaces, Inc.
AgriTitan TM Effective, Safe, & Smart Harvest the Power of Light To Control Bacterial Plant Disease 1 What is AgriTitan TM? AgriTitan TM is a proprietary water-based formulation of extremely small particles
More informationbio-mof-1 DMASM Wavenumber (cm -1 ) Supplementary Figure S1 FTIR spectra of bio-mof-1, DMASMI, and bio-mof-1 DMASM.
bio-mof-1 Transmittance bio-mof-1 DMASM DMASMI 2000 1500 1000 500 Wavenumber (cm -1 ) Supplementary Figure S1 FTIR spectra of bio-mof-1, DMASMI, and bio-mof-1 DMASM. Intensity (a.u.) bio-mof-1 DMASM as
More informationSupporting Information
Supporting Information Size Tunable ZnO Nanoparticles to Enhance Electron Injection in Solution Processed QLEDs Jiangyong Pan,, Jing Chen,, Qianqian Huang, Qasim Khan, Xiang Liu, Zhi Tao, Zichen Zhang,
More informationSupplementary Fig. 1 Atomic force microscopy topography images Two-dimensional atomic force microscopy images (with an area of 1 m 1 m) of Cu and
Supplementary Fig. 1 Atomic force microscopy topography images Two-dimensional atomic force microscopy images (with an area of 1 m 1 m) of Cu and Cu(O = 5.0%) films deposited on 20-nm-thick ZnO films during
More informationSupplementary Fig. 1.
Supplementary Fig. 1. (a,b,e,f) SEM and (c,d,g,h) TEM images of (a-d) TiO 2 mesocrystals and (e-h) NiO mesocrystals. The scale bars in the panel c, d, g, and h are 500, 2, 50, and 5 nm, respectively. SAED
More informationRelative SOD1 activity. Relative SOD2 activity. Relative SOD activity (Infected:Mock) + CP + DDC
Supplementary Figure 1. SOD1 activity is significantly increased relative to SOD1 levels. SOD1 and SOD2 activities in the infected mork13 cells are shown normalised to their corresponding levels and relative
More informationEvidence of intrinsic ferromagnetism in individual dilute magnetic semiconducting nanostructures O-K. (a) Zn-L Zn-L 2,3
SUPPLEMENTARY INFORMATION Evidence of intrinsic ferromagnetism in individual dilute magnetic semiconducting nanostructures O-K (a) O-K Fe-L Co-L 2,3 2,3 Zn-L Zn-L 2,3 2,3 (b) Intensity (a. u.) 500 750
More informationElectronic Supplementary Information (ESI) A unique dansyl-based chromogenic chemosensor for rapid and ultrasensitive hydrazine detection
Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B. This journal is The Royal Society of Chemistry 2014 Electronic Supplementary Information (ESI) A unique dansyl-based chromogenic
More informationSupplementary Materials for
advances.sciencemag.org/cgi/content/full/2/12/e1601838/dc1 Supplementary Materials for General and programmable synthesis of hybrid liposome/metal nanoparticles Jin-Ho Lee, Yonghee Shin, Wooju Lee, Keumrai
More informationGraphene Quantum Dots-Band-Aids Used for Wound Disinfection
Supporting information Graphene Quantum Dots-Band-Aids Used for Wound Disinfection Hanjun Sun, Nan Gao, Kai Dong, Jinsong Ren, and Xiaogang Qu* Laboratory of Chemical Biology, Division of Biological Inorganic
More informationSUPPORTING INFORMATION
SUPPORTING INFORMATION SUPPLEMENTARY FIGURE LEGENDS Fig. S1. Separation of non-dissolved nanoparticles. Tests were conducted on the separation of non-dissolved nanoparticles added in excess to BEGM (A)
More informationFluorescent Carbon Dots as Off-On Nanosensor for Ascorbic Acid
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2014 Fluorescent Carbon Dots as Off-On Nanosensor for Ascorbic Acid Jun Gong, Xin Lu, Xueqin An*
More informationCHAPTER 5 CHARACTERIZATION OF ZINC OXIDE NANO- PARTICLES
88 CHAPTER 5 CHARACTERIZATION OF ZINC OXIDE NANO- PARTICLES 5.1 INTRODUCTION This chapter deals with the characterization of ZnO nano-particles using FTIR, XRD, PSA & SEM. The results analysis and interpretations
More informationCHAPTER 3. PHOTOCATALYTIC DEGRADATION OF METHYLENE BLUE AND ACID RED 18 DYES BY Bi-Au-ZnO
CHAPTER 3 PHOTOCATALYTIC DEGRADATION OF METHYLENE BLUE AND ACID RED 18 DYES BY Bi-Au-ZnO In this chapter, characterization of Bi-Au-ZnO and its photocatalytic activity on the degradation of Methylene Blue
More informationSupporting Information
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2018 Copyright WILEY-VCH Verlag GmbH & Co. KGaA, 69469 Weinheim, Germany, 2016. Supporting Information
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION DOI: 10.1038/NNANO.2012.80 Protein-Inorganic Hybrid Nanoflowers Jun Ge, Jiandu Lei, and Richard N. Zare Supporting Online Material Materials Proteins including albumin from bovine
More informationColor-Tunable and High-Efficiency. Dye-Encapsulated Metal-Organic Framework. (MOF) Composites Used for Smart White
Supporting Information Color-Tunable and High-Efficiency Dye-Encapsulated Metal-Organic Framework (MOF) Composites Used for Smart White LEDs Wenwei Chen a, Yixi Zhuang a, *, Le Wang b, *, Ying Lv a, Jianbin
More informationSimultaneous Blood Brain Barrier Crossing and Protection for Stroke Treatment Based on Edaravone-Loaded Ceria Nanoparticles
Supporting Information Simultaneous Blood Brain Barrier Crossing and Protection for Stroke Treatment Based on Edaravone-Loaded Ceria Nanoparticles Qunqun Bao, Ping Hu, * Yingying Xu, Tiansheng Cheng, Chenyang
More informationSupplementary Information
Supplementary Information Title: Targeted and controlled anticancer drug delivery and release with magnetoelectric nanoparticles Authors: Alexandra Rodzinski 1, Rakesh Guduru 1, Ping Liang 2,3, Ali Hadjikhani
More informationSolNanoTox. How the solubility of nanoparticles impacts on their behaviour and their potential toxicity? Valérie FESSARD
SolNanoTox How the solubility of nanoparticles impacts on their behaviour and their potential toxicity? Valérie FESSARD Program between France and Germany Up to 42 months; from March 2014 up to August
More informationIn reality the PDT mechanism requires that three things be together in the body at the same time. These three things are oxygen, chromophore
The Real Skinny on Green Light Therapy This is a dry read...but try and make it through! I've underlined a couple of sentences that I really want you take in. 1. Overview. Current phototherapy for acne
More informationSupporting information. Precise Photodynamic Therapy of Cancer via Subcellular Dynamic Tracing of Dual-loaded Upconversion Nanophotosensitizers
Supporting information Precise Photodynamic Therapy of Cancer via Subcellular Dynamic Tracing of Dual-loaded Upconversion Nanophotosensitizers Yulei Chang 1, Xiaodan Li 1,3, Li zhang 1,3, Lu Xia 1, Xiaomin
More informationEFFECT OF SOLVENTS ON PARTICLE STRUCTURE, MORPHOLOGY AND OPTICAL PROPERTIES OF ZINC OXIDE NANOPARTICLES
EFFECT OF SOLVENTS ON PARTICLE STRUCTURE, MORPHOLOGY AND OPTICAL PROPERTIES OF ZINC OXIDE NANOPARTICLES A.Vanaja 1 and K.Srinivasa Rao 2 1 Department of Physics, Lingayya s University, Old Faridabad, Haryana,
More informationOncolytic Adenovirus Complexes Coated with Lipids and Calcium Phosphate for Cancer Gene Therapy
Oncolytic Adenovirus Complexes Coated with Lipids and Calcium Phosphate for Cancer Gene Therapy Jianhua Chen, Pei Gao, Sujing Yuan, Rongxin Li, Aimin Ni, Liang Chu, Li Ding, Ying Sun, Xin-Yuan Liu, Yourong
More informationSupplementary Information for
Supplementary Information for Acid-Resistant Mesoporous Metal Organic Framework Toward Oral Insulin Delivery: Protein Encapsulation, Protection & Release Yijing Chen, Peng Li, Justin A. Modica, Riki J.
More informationAnanya Baksi CY11D042
Insulin-Directed Synthesis of Fluorescent Gold Nanoclusters: Preservation of Insulin Bioactivity and Versatility in Cell Imaging Angew. Chem. Int. Ed. 2011, 50, 7056 7060 Chien-Liang Liu, Hung-Tsung Wu,
More informationSupporting Information
Supporting Information Cancer Cell Membrane-Biomimetic Nanoprobes with Two-Photon Excitation and Near-Infrared Emission for Intravital Tumor Fluorescence Imaging Yanlin Lv 1,2,, Ming Liu 3,4,, Yong Zhang
More informationCharacterization of ZnO/TiO 2 Nanocomposites Prepared via the Sol-Gel Method
Journal of the Korean Ceramic Society Vol. 55, No., pp. 140~144, 018. https://doi.org/10.4191/kcers.018.55..10 Communication Characterization of ZnO/TiO Nanocomposites Prepared via the Sol-Gel Method Nalumaga
More informationSupporting Information
Supporting Information Development of a Highly Sensitive Fluorescence Probe for Hydrogen Peroxide Masahiro Abo,, Yasuteru Urano, Kenjiro Hanaoka,, Takuya Terai,, Toru Komatsu, and Tetsuo Nagano,, * Graduate
More informationSupporting Information. Solar Cells and the Improvement on Open-Circuit. Voltage
Supporting Information Zn 2 SnO 4 Nanowires as Photoanode for Dye Sensitized Solar Cells and the Improvement on Open-Circuit Voltage Jiajun Chen, Liyou Lu, and Wenyong Wang* Department of Physics and Astronomy,
More informationFe-doped ZnO synthesized by parallel flow precipitation process for improving photocatalytic activity
IOP Conference Series: Materials Science and Engineering PAPER OPEN ACCESS Fe-doped ZnO synthesized by parallel flow precipitation process for improving photocatalytic activity To cite this article: Q
More informationPhoto-reduction and Stabilization Capability of Molecular Weight. Fractionated Natural Organic Matter in Transformation of Silver Ion to
Supporting information Photo-reduction and Stabilization Capability of Molecular Weight Fractionated Natural Organic Matter in Transformation of Silver Ion to Metallic Nanoparticle 7 8 9 Yongguang Yin
More informationSupporting Information. Light-enhanced hypoxia-response of conjugated polymer nanocarrier. for successive synergistic photodynamic and chemo-therapy
Supporting Information Light-enhanced hypoxia-response of conjugated polymer nanocarrier for successive synergistic photodynamic and chemo-therapy Xiaolong Zhang a,d, Ming Wu a,d, Jiong Li a,c,d, Shanyou
More informationSupporting Information. Non-thermal plasma with 2-deoxy-D-glucose synergistically induces cell death by targeting glycolysis in blood cancer cells
Supporting Information Non-thermal plasma with 2-deoxy-D-glucose synergistically induces cell death by targeting glycolysis in blood cancer cells Neha Kaushik, 1 Su Jae Lee, 2 Tae Gyu Choi 3, Ku Youn Baik,
More informationMonitoring Tumor Therapy
Monitoring Tumor Therapy Zenalux Biomedical, Inc. The Zenascope was used to monitor the physiological response to a vascular disrupting agent in mouse tumors. Changes in hemoglobin concentration, hemoglobin
More informationA highly selective AIE fluorogen for lipid droplet imaging in live cells and green algae
Electronic Supporting Information highly selective IE fluorogen for lipid droplet imaging in live cells and green algae Erjing Wang, ab Engui Zhao, ab Yuning Hong, ab Jacky W. Y. Lam, ab and en Zhong Tang*
More informationSynergistic effects of antioxidative peptides from rice bran
Synergistic effects of antioxidative peptides from rice bran Pichamon Kiatwuthinon 1,*, Neeracha Lapanusorn 1, Anunyaporn Phungsom 1, Wirawan Tinanchai 1 1 Department of Biochemistry, Faculty of Science,
More informationCharacterization of ZnO:Cu Nanoparticles by Photoluminescence Technique
Characterization of ZnO:Cu Nanoparticles by Photoluminescence Technique Binildev R 1, Hareesh P S 2, Shilpa Prasad 3, Saravana Kumar 4 1,2,3 Department of Physics, Sree Narayana College Chengannur 4 Department
More informationSafer by design, transparent, UV-absorbing ZnO nanorods with minimal genotoxicity
Safer by design, transparent, UV-absorbing ZnO nanorods with minimal genotoxicity Georgios A. Sotiriou, Christa Watson, Kim M. Murdaugh, Alison Elder 1 and Philip Demokritou Center for Nanotechnology and
More informationDesign and Development of Surface Modification and Synthesis Strategies to Reduce Toxicity of Nanoparticles
Design and Development of Surface Modification and Synthesis Strategies to Reduce Toxicity of Nanoparticles Seda Keleştemur Genetics and Bioengineering Department Yeditepe University Istanbul, Turkey Outline
More informationshehab Moh Tarek ... ManarHajeer
3 shehab Moh Tarek... ManarHajeer In the previous lecture we discussed the accumulation of oxygen- derived free radicals as a mechanism of cell injury, we covered their production and their pathologic
More informationSupplementary Figure 1. EL spectral characteristics. (a)
Supplementary Figure 1. EL spectral characteristics. (a) Angular dependence of normalized light intensity at 540 nm according to ZnO layers; Lambertian emission pattern (black square), ZnO-F (red circle),
More informationRisk assessment of TiO 2 nanoparticles via oral exposure, including toxicokinetic considerations
1 Risk assessment of TiO 2 nanoparticles via oral exposure, including toxicokinetic considerations This presentation Overview main findings Relevance to safety assessment of E171 2 Overview of main findings:
More informationA549 and A549-fLuc cells were maintained in high glucose Dulbecco modified
Cell culture and animal model A549 and A549-fLuc cells were maintained in high glucose Dulbecco modified Eagle medium supplemented with 10% fetal bovine serum at 37 C in humidified atmosphere containing
More informationMetal swap between Zn 7 metallothionein 3 and amyloid β Cu protects against amyloid β toxicity
Metal swap between Zn 7 metallothionein 3 and amyloid β Cu protects against amyloid β toxicity Supplementary Information Gabriele Meloni 1, Vanessa Sonois 2,3, Tamara Delaine 2, Luc Guilloreau 2, Audrey
More informationSupporting Information For
Supporting Information For MicroRNA-Catalyzed Cancer Therapeutics Based on DNA-Programmed Nanoparticle Complex Xucheng Luo, 1 Zhi Li, 1 Ganglin Wang, 1 Xuewen He, 2,3 Xiaoqin Shen, 1 Quanhong Sun, 1 Li
More informationSupporting Information
Supporting Information H 2 O 2 -Activatable and O 2 -Evolving Nanoparticles for Highly Efficient and Selective Photodynamic Therapy against Hypoxic Tumor Cells Huachao Chen, Jiangwei Tian, Weijiang He,*
More informationSupplementary Material
Supplementary Material High Photo-Electrochemical Activity of Thylakoid-Carbon Nanotube Composites for Photosynthetic Energy Conversion Jessica O. Calkins a, Yogeswaran Umasankar a, Hugh O Neill b and
More informationSupplementary Figure S1. Statistical measurements on particle size and aspect ratio of
Supplementary Figure S1. Statistical measurements on particle size and aspect ratio of as-prepared Cd 0.5 Zn 0.5 S nanocrystals. a,b,c, Histograms of the particle size distribution of the three-type of
More informationX P. Supplementary Figure 1. Nature Medicine: doi: /nm Nilotinib LSK LT-HSC. Cytoplasm. Cytoplasm. Nucleus. Nucleus
a b c Supplementary Figure 1 c-kit-apc-eflu780 Lin-FITC Flt3-Linc-Kit-APC-eflu780 LSK Sca-1-PE-Cy7 d e f CD48-APC LT-HSC CD150-PerCP-cy5.5 g h i j Cytoplasm RCC1 X Exp 5 mir 126 SPRED1 SPRED1 RAN P SPRED1
More informationCurrent Opinions on the Human Safety of Titanium Dioxide and Zinc Oxide
Current Opinions on the Human Safety of Titanium Dioxide and Zinc Oxide J F. Nash, Ph.D. Central Product Safety The Procter & Gamble Company Cincinnati, OH 45241 nash.jf@pg.com Human Safety: Objective
More informationSupplementary Information
Supplementary Information for Chemical Synthesis of Blue-emitting Metallic Zinc Nano-hexagons Nguyen T. Mai, Trinh T. Thuy, Derrick M. Mott and Shinya Maenosono* School of Materials Science, Japan Advanced
More informationEngineering the Growth of TiO 2 Nanotube Arrays on Flexible Carbon Fibre Sheets
Engineering the Growth of TiO 2 Nanotube Arrays on Flexible Carbon Fibre Sheets Peng Chen, a Li Gu, b Xiudong Xue, a Mingjuan Li a and Xuebo Cao* a a Key Lab of Organic Synthesis of Jiangsu Province and
More informationSupporting Information. Light Emitting Photon Upconversion Nanoparticles. in the Generation of Transdermal Reactive Oxygen.
Supporting Information Light Emitting Photon Upconversion Nanoparticles in the Generation of Transdermal Reactive Oxygen Species Martin Prieto a,, Alina Y. Rwei c, d,, Teresa Alejo a, Tuo Wei c, Maria
More informationENHANCED NIR RADIATION-TRIGGERED HYPERTHERMIA BY MITOCHONDRIAL TARGETING
ENHANCED NIR RADIATION-TRIGGERED HYPERTHERMIA BY MITOCHONDRIAL TARGETING J. Am. Chem. Soc., 2015, 137, 3017-3023 Tanja Krainz Current Literature December 19, 2015 What is Hyperthermia? Hyperthermia (also
More informationof Serum Oxidation V max ) and
SUPPLEMENTAL DATA Paradoxical Effects of Serum Amyloid A on the Lipoprotein Oxidation Suggest a New Antioxidant t Function for SAA Shobini Jayaraman, Christian Haupt, and Olga Gursky Figure S1. Lipid peroxidation
More informationMechanochemical Doping of a Non-Metal Element into Zinc Oxide
Chemistry for Sustainable Development 15 (2007) 249 253 249 Mechanochemical Doping of a Non-Metal Element into Zinc Oxide J. WANG, J. F. LU, Q. W. ZHANG, S. YIN, T. SATO and F. SAITO Institute of Multidisciplinary
More informationPhotocatalytic removing of methylene blue by using of Cu-doped ZnO, Ag-doped ZnO and Cu,Ag-codoped ZnO nanostructures
Photocatalytic removing of methylene blue by using of Cu-doped ZnO, Ag-doped ZnO and Cu,Ag-codoped ZnO nanostructures Rahmatollah Rahimi*, Javad Shokrayian, Mahboubeh Rabbani Department of Chemistry, Iran
More informationSupporting Information Appendix
Repeatable and adjustable on-demand sciatic nerve block with phototriggerable liposomes Alina Y. Rwei, Jung-Jae Lee, Changyou Zhan, Qian Liu, Meryem T. Ok, Sahadev A. Shankarappa, Robert Langer, Daniel
More informationMembrane Fluidity Changes Are Associated with Benzo[a]Pyrene-Induced Apoptosis in F258 Cells
Membrane Fluidity Changes Are Associated with Benzo[a]Pyrene-Induced Apoptosis in F258 Cells Protection by Exogenous Cholesterol MORGANE GORRIA, a XAVIER TEKPLI, a ODILE SERGENT, b LAURENCE HUC, a FRANÇOIS
More informationNanopigments For Broad Spectrum Sun Protection. Patricia Aikens NAFTA Technical Services
Nanopigments For Broad Spectrum Sun Protection Patricia Aikens NAFTA Technical Services UV radiation and the skin UV absorbers Nanopigments Titanium dioxide Zinc Oxide Electromagnetic Radiation Longer
More informationSupporting Information
Electronic Supplementary Material (ESI) for Journal of Materials Chemistry A. This journal is The Royal Society of Chemistry 2014 Supporting Information Remarkable improvement in visible-light induced
More informationNature Protocols: doi: /nprot Supplementary Figure 1
Supplementary Figure 1 Influence of ZnCl 2 concentration on ph of 50 (gray) and 100 mm (orange) Tris-HCl buffer solutions. The green line represents the initial value of the ph of Tris buffers. Supplementary
More informationProtein Cleavage Due to Pro-oxidative Activity in Some Spices
Protein Cleavage Due to Pro-oxidative Activity in Some Spices Sittiwat Lertsiri Department of Biotechnology Faculty of Science, Mahidol University Phayathai, Bangkok 10400 Thailand Kanchana Dumri Department
More informationAg Nano Particles. Mapping overlay of HaCaT cells incubated with Ag NP sample: Red maps each pixel matching the spectral curves of Ag NP
Ag Nano Particles Nano-scale Microscopy & Hyperspectral Imaging Ag Nano Particles Family of Spectral Curves HaCaT cells incubated with Ag NP Hyperspectral Scanned Image: 40x objective Mapping overlay of
More informationSupplementary Material
Supplementary Material Materials and methods Enzyme assay The enzymatic activity of -glucosidase toward salicin was measured with the Miller method (Miller, 1959) using glucose as the standard. A total
More information- 1 - Cell types Monocytes THP-1 cells Macrophages. LPS Treatment time (Hour) IL-6 level (pg/ml)
Supplementary Table ST1: The dynamic effect of LPS on IL-6 production in monocytes and THP-1 cells after GdA treatment. Monocytes, THP-1 cells and macrophages (5x10 5 ) were incubated with 10 μg/ml of
More informationSol-Gel Synthesis of Zinc Oxide (ZnO) Nanoparticles: Study of Structural and Optical Properties
Journal of Sciences, Islamic Republic of Iran 26(3): 281-285 (2015) University of Tehran, ISSN 1016-1104 http://jsciences.ut.ac.ir Sol-Gel Synthesis of Zinc Oxide (ZnO) Nanoparticles: Study of Structural
More informationSupplementary Information
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2018 Supplementary Information Photoreduction Route for Cu 2 O/TiO 2 Nanotubes Junction for Enhanced
More informationAN ACTIVE SHELTER AGAINST POLLUTION V.16
AN ACTIVE SHELTER AGAINST POLLUTION V.16 THE SKIN IS CONSTANTLY EXPOSED The skin is an interface and thus is constantly exposed to environmental agents: Sun radiation Smoke Air pollution Free radicals
More informationNano-analytical electron microscopy reveals fundamental insights into human cardiovascular tissue calcification
Nano-analytical electron microscopy reveals fundamental insights into human cardiovascular tissue calcification Supplementary table 1: Aortic valve donor information (n=32). Patient age (yr) 57±11 (range:
More informationMembrane lipid peroxidation by ultrasound: Mechanism and implications
J. Biosci., Vol. 15, Number 3, September 1990, pp. 211 215. Printed in India. Membrane lipid peroxidation by ultrasound: Mechanism and implications A. K. JANA, S. AGARWAL and S. N. CHATTERJEE* Biophysics
More informationROS scavenging Mn 3 O 4 nanozymes for in vivo anti-inflammation
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2018 Supporting Information ROS scavenging Mn 3 O 4 nanozymes for in vivo anti-inflammation
More informationElectronic Supplementary Information
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2016 Electronic Supplementary Information MnO 2 -induced synthesis of fluorescent polydopamine nanparticles
More informationMultimodal Spectroscopic Tissue Scanner for diagnosis of ex vivo surgical specimens
Multimodal Spectroscopic Tissue Scanner for diagnosis of ex vivo surgical specimens Collaborating researcher: Dr. Maryann Fitzmaurice (Case Western Reserve University, Cleveland, OH), Dr. Tulio Valdez
More informationPhotoacoustic Imaging and Therapy in Biomedicine. Nicholas Tobey and Grace Yook. Optical Engineering. Dr. Kasra Daneshvar
Photoacoustic Imaging 1 Photoacoustic Imaging and Therapy in Biomedicine Nicholas Tobey and Grace Yook Optical Engineering Dr. Kasra Daneshvar July 16, 2010 Photoacoustic Imaging 2 Abstract When a pulsed
More informationMetal oxide sunscreens protect skin by absorption, not by reflection or scattering
Photodermatology, Photoimmunology & Photomedicine ORIGINAL ARTICLE Metal oxide sunscreens protect skin by absorption, not by reflection or scattering Curtis Cole 1, Thomas Shyr 2 & Hao Ou-Yang 2 1 Sun
More informationElectronic Supplementary Information
Electronic Supplementary Material (ESI) for Chemical Communications. This journal is The Royal Society of Chemistry 2018 Electronic Supplementary Information A ratiometric fluorescence probe for peroxynitrite
More informationDense and Dynamic Polyethylene Glycol Shells Cloak Nanoparticles. from Uptake by Liver Endothelial Cells for Long Blood Circulation
Dense and Dynamic Polyethylene Glycol Shells Cloak Nanoparticles from Uptake by Liver Endothelial Cells for Long Blood Circulation Hao Zhou, Zhiyuan Fan, Peter Y. Li, Junjie Deng,, Dimitrios C. Arhontoulis,
More informationRESEARCH INTEREST INTRODUCTION:
RESEARCH INTEREST INTRODUCTION: Cancer is regarded as one among the dreadful diseases. Despite of much advancement in the theranostics of cancer, the cure rate for this terrible disease without any side
More informationABSTRACT OF Ph.D. THESIS IN VIVO DETECTION OF LIGHT STRESS INDUCED REACTIVE OXYGEN SPECIES IN PLANTS. Ph.D. Thesis. Csengele Barta
ABSTRACT OF Ph.D. THESIS IN VIVO DETECTION OF LIGHT STRESS INDUCED REACTIVE OXYGEN SPECIES IN PLANTS Ph.D. Thesis Csengele Barta Supervisor: Dr. Éva Hideg Biological Research Centre of the Hungarian Academy
More informationSupplementary information - Table (1), Figures (12), and Videos (5)
Supplementary information - Table (1), Figures (12), and Videos (5) A soft, transparent, freely accessible cranial window for chronic imaging and electrophysiology Chaejeong Heo 1, Hyejin Park 1, 2, Yong-Tae
More informationSupporting Information
Supporting Information Over cd/a Efficient Green Quantum Dot Electroluminescent Device Comprising Uniquely Large-Sized Quantum Dot Ki-Heon Lee, Jeong-Hoon Lee, Hee-Don Kang, Byoungnam Park, Yongwoo Kwon,
More informationSOLAR PHOTOLYSIS AND PHOTOCATALYTIC TREATMENT OF TEXTILE INDUSTRIAL WASTEWATER
Int. J. Chem. Sci.: 8(3), 2010, 1409-1420 SOLAR PHOTOLYSIS AND PHOTOCATALYTIC TREATMENT OF TEXTILE INDUSTRIAL WASTEWATER FALAH H. HUSSEIN * and THEKRA A. ABASS a Department of Chemistry, College of Science,
More informationEstimation of protein using standard curve & Biochemistry analyzer. Experiment 1 & 2 BBT 314 ACh
Estimation of protein using standard curve & Biochemistry analyzer Experiment 1 & 2 BBT 314 ACh Introduction Proteins are an abundant component in all cells, and almost all except storage proteins are
More informationFree Radicals in Biology and Medicine
Free Radicals in Biology and Medicine 0 \ Second Edition BARRY HALLIWELL Professor of Medical Biochemistry, University of London King's College and JOHN M.C. GUTTERIDGE Senior Scientist, National Institute
More informationSupporting Information for:
Supporting Information for: A Robust Liposomal Platform for Direct Colorimetric Detection of Sphingomyelinase Enzyme and Inhibitors Margaret N. Holme, 1,2,3,4 Subinoy Rana, 1,2,3,5 Hanna M. G. Barriga,
More informationEnhanced NIR-I Emission from Water-Dispersible NIR-II Dye-sensitized Core/Active Shell Upconverting Nanoparticles
Electronic Supplementary Material (ESI) for Journal of Materials Chemistry C. This journal is The Royal Society of Chemistry 2018 Supporting Information Enhanced NIR-I Emission from Water-Dispersible NIR-II
More informationReagent-Free Electrophoretic Synthesis of Few-Atom- Thick Metal Oxide Nanosheets
Supporting Information Reagent-Free Electrophoretic Synthesis of Few-Atom- Thick Metal Oxide Nanosheets Chengyi Hou,*,, Minwei Zhang, Lili Zhang, Yingying Tang, Hongzhi Wang, and Qijin Chi*, State Key
More informationTargeted Chemoprevention of Progressive Marrow Failure in Patients with Fanconi Anemia using Quercetin, a Pilot study
Targeted Chemoprevention of Progressive Marrow Failure in Patients with Fanconi Anemia using Quercetin, a Pilot study Parinda Mehta, M.D. Division of Bone Marrow Transplantation and Immune Deficiency Cincinnati
More informationSurface-Enhanced Raman Scattering Active Gold Nanoparticles. with Enzyme-Mimicking Activities for Measuring Glucose and
Surface-Enhanced Raman Scattering Active Gold Nanoparticles with Enzyme-Mimicking Activities for Measuring Glucose and Lactate in Living Tissues Yihui Hu, Hanjun Cheng, Xiaozhi Zhao, Jiangjiexing Wu, Faheem
More information