Development of a VP6 subunit rotavirus vaccine A dual role of VP6 as a vaccine antigen and an adjuvant
|
|
- Reynard Lamb
- 5 years ago
- Views:
Transcription
1 30 August 2018, Minsk 13TH INTERNATIONAL ROTAVIRUS SYMPOSIUM Development of a VP6 subunit rotavirus vaccine A dual role of VP6 as a vaccine antigen and an adjuvant Dr. Vesna Blazevic Head of Laboratory Vaccine Research Center University of Tampere 1
2 Rotavirus (RV) VP6 RV VP6 Triple-layered RV particle: 1. Inner capsid VP2 2. Intermediate capsid VP6 Highly conserved protein Groups (A-H) Subgroups (SGI, II, I+II, non I/II) 3. Outer capsid VP7 with VP4 spikes -> a dual role of VP6 as a subunit rotavirus vaccine and an adjuvant in a combination norovirus (NoV) + RV vaccine candidate developed by our laboratory 2
3 Rationales for a non-live subunit RV vaccine No adverse issues of live RV vaccines No competition of uptake with enteric viruses in the gut Live RV vaccines (RV1 and RV5) show lower efficacy in Africa and Asia Live RV vaccines show decline of efficacy in the 2nd year in developing countries Genetic background of the population not critical (e.g. secretors/nonsecretors) 3
4 Combination NoV+RV vaccine: recombinant NoV virus-like particles (VLPs) + RV VP6 Non-live subunit vaccine against childhood gastroenteritis All vaccine antigens are oligomeric proteins produced in insect cells by recombinant baculovirus expression vector system (BEVS) NoV VP1 RV VP6 VP2 roteins/rota%20cut-away.gif ~100 nm capsid protein VP1 BEVS capsid protein VP6 + capsid VP2 or or 100 nm 100 nm NoV VLPs ~30-40 nm VP6 nanotubes (VP6T) ~75 nm, µm long (ph 7) VP6 nanospheres (VP6S) ~75 nm (ph 4) Double-layered 2/6-VLPs ~65 nm M. Estes et al., J Virology 1987 (rvp6 produced in BV expression system) 4
5 Trivalent combination vaccine formulations -extensively studied in our laboratory NoV GII.4 VLPs + GI.3 VLPs + RV VP6 oligomers (VP6T or VP6S or dlvlps) GII.4 VLPs GII.4 VLPs GI.3 VLPs GI.3 VLPs RV rvp6 VP6 T dlvlps a representative of GI and GII NoVs no external adjuvants! 5
6 The combination vaccine induces strong NoV and RV type-specific and cross-reactive immunity PLOS One Jul 26;8(7):e VP6-specific serum IgG VP6-specific mucosal IgG VP6-specific mucosal IgA OD 490nm 2.5 Trivalent (GII-4+GI-3+VP6) OD 490nm VP6 IM VP6 IN Control IM Control IN VP6 IM VP6 IN Control IM Control IN Sample dilution Sample dilution Strong cross-reactive serum antibodies to heterologous RV strains Low levels of IgA in feces of IM immunized mice Rapid increase after live RV challenge 6
7 VP6 protects mice from live RV EDIM challenge RV antigen shedding OD 450nm VP6 IN VP6 IM Control 66 % reduction 65 % reduction Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Day 8 Days post-challenge Protection correlated with VP6- specific serum IgA levels >50 % reduction in RV antigen shedding in the stool considered significant protection from virus challenge 7
8 Mechanisms of VP6 protection No traditional neutralizing antibodies, instead non-serotype specific protective immunity VP6-specific cytotoxic T lymphocytes (CD8 + T cells) (H Greenberg s group) VP6-specific CD4 + T cells (R Ward s group) Direct cytotoxic mechanism and anti-viral cytokines 50 polymeric/secretory IgA antibodies (H Greenberg, J Crowe) Intracellular neutralization by VP6-specific IgA antibodies following transcytosis VP6-specific piga inhibits RV replication at the transcription level by blocking channels on dlrv particles and preventing RV mrna release (Aiyegbo et al. 2013, 2014) 8
9 Correlates of protection in children VP6 RV-specific serum IgA antibody levels were shown to correlate to RV1 and RV5 vaccines efficacy (Patel et al. 2013) VP6-specific serum IgA in children years Serum IgA against rvp r=0.683 (p<0.0001) Serum IgA against Wa (Lappalainen et al. 2016) Early live bovine RV vaccine RIT4237 induced the strongest response against VP6 (Svensson et al. 1987) Could VP6 induced immunity be responsible for most of the protection against severe RV AGE? 9
10 VP6 adjuvant effect on NoV immunity Study design VLP IM T µg NoV VLP + VP6 VLP VP6T/S + co-delivery (mix) NoV GII.4-specific serum IgG NoV GII.4 -specific T cell responses OD 490nm µg GII-4 10µg GII-4 0.3µg GII µg VP6 1:100 1:200 1:400 1:800 1:1600 1:3200 1:6400 1: : : : : : : Serum Dilution IFN-γ SFC / 10 6 splenocytes (Blazevic et al. 2015, Malm et al. 2017) 0 GII.4 peptide pool OVA peptide 0.3µg VLP 0.3µg VLP + 10µg VP6T 0.3µg VLP + 10µg VP6S Experimental Group Ctrl 10
11 RV VP6 acts as a delivery vehicle for NoV VLPs 0.3 µg GII.4 VLP 10 µg VP6 T 3.0 NoV GII.4 -specific IgG titers co-delivery 1h between injections contralateral sites OD 490 nm T Serum dilution IM (Malm et al. 2017) 11
12 VP6 is internalized by antigen presenting cells (APC) RAW ibmdc RV VP6 CM Anti-VP6 FITC Anti-VP6-FITC Intracellular staining with anti-vp6-fitc ab shows that VP6 is internalized by macrophages and dendritic cells (DC) 12
13 RV VP6 activates macrophages and DC CM VP6 TNF-a (pg/ml) TNF-α production by RAW cells CM BSA 50 ug/ml VP6 100 ug/ml 5000 Mean fluorescence intensity (MFI) CM BSA VP6 VP6 % increase MHC II CD IL-6 (pg/ml) 0 IL-6 production by JAWSII cells 3000 CM BSA 50 ug/ml 2000 VP6 100 ug/ml 1000 increased cell surface expression of antigen presentation and co-stimulatory molecules 0 24h (Malm et al. 2016) Induced cytokine production by macrophages and DC 13
14 Conclusions VP6 VP6 T VP6 S RV VP6-specific immune response protects from heterologous RV challenge in mice Importance of non-serotype-specific immunity induced by VP6 in protection from RV infection RV VP6 acts as an adjuvant for norovirus VLPs RV VP6 activates antigen presenting cells (APC) RV VP6 is an excellent subunit vaccine candidate against RV 14
15 Acknowledgements Vaccine Research Center University of Tampere, Finland Suvi Heinimäki Maria Malm Kirsi Tamminen Eeva Jokela Sanna Kavén Nina Koivisto Timo Vesikari Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, México Ana Ruth Pastor Laura A. Palomares Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Morelos, México Vanessa López-Guerrero Fernando Esquivel-Guadarrama Thank you 15
Status of Vaccine Research and Development for Norovirus Prepared for WHO PD-VAC
Status of Vaccine Research and Development for Norovirus Prepared for WHO PD-VAC I. About the Disease and Pathogen Basic information on pathogen, including transmission, estimated global disease burden
More informationNoroviruses. Duncan Steele Bill & Melinda Gates Foundation. Acknowledgements: Ben Lopman and Umesh Parashar, CDC Megan Carey and Julia Bosch, BMGF
Noroviruses Duncan Steele Bill & Melinda Gates Foundation Acknowledgements: Ben Lopman and Umesh Parashar, CDC Megan Carey and Julia Bosch, BMGF 1 Global norovirus burden Globally, norovirus is associated
More informationESCMID Online Lecture Library
Vaccines against norovirus state of the art, trials in children and adults Hugues Bogaerts MD global vaccine consultant at H+B 3rd ESCMID Conference on Vaccines 1 Between Jan and 22 Dec 2014, 689 outbreaks
More informationFinnish Vaccine Trial Network Collaboration with NordicPedMed?
2nd Nordic Conference on Pediatric Medicines Helsinki 21 May 2018 Finland Finnish Vaccine Trial Network Collaboration with NordicPedMed? Tampere Prof. Timo Vesikari Vaccine Research Center University of
More informationDevelopment of T cell immunity to norovirus and rotavirus in children under five years of age
https://helda.helsinki.fi Development of T cell immunity to norovirus and rotavirus in children under five years of age Malm, Maria 2019-03-01 Malm, M, Hyoty, H, Knip, M, Vesikari, T & Blazevic, V 2019,
More informationMultiple consecutive norovirus infections in the first 2 years of life
DOI 10.1007/s00431-015-2591-8 SHORT COMMUNICATION Multiple consecutive norovirus infections in the first 2 years of life Vesna Blazevic 1 & Maria Malm 1 & Marjo Salminen 1 & Sami Oikarinen 2 & Heikki Hyöty
More informationLongitudinal Studies of Neutralizing Antibody Responses to Rotavirus in Stools and Sera of Children following Severe Rotavirus Gastroenteritis
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, Nov. 1998, p. 897 901 Vol. 5, No. 6 1071-412X/98/$04.00 0 Copyright 1998, American Society for Microbiology. All Rights Reserved. Longitudinal Studies of
More informationRotavirus capsid VP6 tubular and spherical nanostructures act as local adjuvants when co-delivered with norovirus VLPs
Clinical and Experimental Immunology ORIGINAL ARTICLE doi:10.1111/cei.12977 Rotavirus capsid VP6 tubular and spherical nanostructures act as local adjuvants when co-delivered with norovirus VLPs M. Malm,
More informationZheng, BJ; Du, LY; Zhao, GY; Lin, YP; Sui, HY; Chan, C; Ma, S; Guan, Y; Yuen, KY. Citation Hong Kong Medical Journal, 2008, v. 14 suppl. 4, p.
Title Studies of SARS virus vaccines Author(s) Zheng, BJ; Du, LY; Zhao, GY; Lin, YP; Sui, HY; Chan, C; Ma, S; Guan, Y; Yuen, KY Citation Hong Kong Medical Journal, 2008, v. 14 suppl. 4, p. 39-43 Issued
More informationThe humoral immune responses to IBV proteins.
The humoral immune responses to IBV proteins. E. Dan Heller and Rosa Meir The Hebrew University of Jerusalem, Israel COST FA1207 meeting WG2 + WG3, Budapest, Jan. 2015 1 IBV encodes four major structural
More informationFoot and Mouth Disease Vaccine Research and Development in India
Foot and Mouth Disease Vaccine Research and Development in India R.Venkataramanan Indian Veterinary Research Institute, Hebbal, Bangalore 560 024 Foot and Mouth Disease in India Present Status Large Susceptible
More informationGastroenteritis Viruses Prof. Mary K. Estes
Gastroenteritis Viruses Mary K. Estes, Ph.D. Professor of Molecular Virology and Microbiology and Medicine-GI, BCM Director, Texas Medical Center DDC 1 Outline Gastrointestinal viruses Rotaviruses Noroviruses
More informationRAPID COMMUNICATION. Integrin 2 1 Mediates the Cell Attachment of the Rotavirus Neuraminidase-Resistant Variant nar3
Virology 278, 50 54 (2000) doi:10.1006/viro.2000.0660, available online at http://www.idealibrary.com on RAPID COMMUNICATION Integrin 2 1 Mediates the Cell Attachment of the Rotavirus Neuraminidase-Resistant
More informationSupporting Information
Supporting Information Valkenburg et al. 10.1073/pnas.1403684111 SI Materials and Methods ELISA and Microneutralization. Sera were treated with Receptor Destroying Enzyme II (RDE II, Accurate) before ELISA
More informationHigh Serum Levels of Norovirus Genotype Specific Blocking Antibodies Correlate With Protection From Infection in Children
MAJOR ARTICLE High Serum Levels of Norovirus Genotype Specific Blocking Antibodies Correlate With Protection From Infection in Children Maria Malm, 1 Hanni Uusi-Kerttula, 1 Timo Vesikari, 1,2 and Vesna
More informationViral Agents of Paediatric Gastroenteritis
Viral Agents of Paediatric Gastroenteritis Dr Carl Kirkwood -------------------- Enteric Virus Research Group Murdoch Childrens Research Institute Royal Children s Hospital Victoria. WHO Collaborating
More informationRabies virus-like particles expressed in HEK293 cells
Engineering Conferences International ECI Digital Archives Vaccine Technology IV Proceedings Spring 5-21-2012 Rabies virus-like particles expressed in HEK293 cells Diego Fontana Cell Culture Laboratory
More informationCationic Liposome-DNA Complexes as Immunomodulators and Vaccine Adjuvants
Cationic Liposome-DNA Complexes as Immunomodulators and Vaccine Adjuvants September 29, 28 Platform Technology Proprietary Cationic Lipid-DNA Complexes Cationic/Neutral Lipid + DNA = JVRS-1 JVRS-1 + Antigen
More informationVIRAL AGENTS CAUSING GASTROENTERITIS
VIRAL AGENTS CAUSING GASTROENTERITIS VIRAL AGENTS CAUSING GASTROENTERITIS Pathogens discussed in our lectures 1. Rotavirus 2. Enteric adenoviruses 3. Caliciviruses 4. Astroviruses 5. Toroviruses Viruses
More informationProphylactic HPV Vaccines. Margaret Stanley Department of Pathology Cambridge
Prophylactic HPV Vaccines Margaret Stanley Department of Pathology Cambridge 8kb double stranded DNA viruses, absolutely host and tissue specific, Can t grow virus in tissue culture Classified by genotype
More informationStudying Repeated Immunization in an Animal Model. Kanta Subbarao Laboratory of Infectious Diseases, NIAID
Studying Repeated Immunization in an Animal Model Kanta Subbarao Laboratory of Infectious Diseases, NIAID Animal models in Influenza Research Commonly used Mice Ferrets Guinea pigs Non human primates Less
More informationA Multicistronic DNA Vaccine Induces Significant Protection against Tuberculosis in Mice and Offers Flexibility in the Expressed Antigen Repertoire.
Company LOGO A Multicistronic DNA Vaccine Induces Significant Protection against Tuberculosis in Mice and Offers Flexibility in the Expressed Antigen Repertoire. Fayaz Ahmad Mir, Stefan H. E. Kaufmann,
More informationAntigen Presentation to T lymphocytes
Antigen Presentation to T lymphocytes Immunology 441 Lectures 6 & 7 Chapter 6 October 10 & 12, 2016 Jessica Hamerman jhamerman@benaroyaresearch.org Office hours by arrangement Antibodies and T cell receptors
More informationGenetic and mechanistic Determinants of Rotavirus Host Range Restriction
Genetic and mechanistic Determinants of Rotavirus Host Range Restriction Harry Greenberg MD and the Greenberg Lab Stanford University School of Medicine Eleventh International Rotavirus Symposium New Delhi,
More informationClinical Trials of Pandemic Vaccines: Key Issues. John Treanor University of Rochester Rochester, NY
Clinical Trials of Pandemic Vaccines: Key Issues John Treanor University of Rochester Rochester, NY Inactivated vaccine approach Proven technology Used successfully in 1957 and 1968 Abundant efficacy data
More informationImmune Response to Rotavirus Polypeptides after Vaccination with Heterologous Rotavirus Vaccines (RIT 4237, RRV-1)
J gen Virol (1987), 68, 1993 1999 Printed in Great Britain 1993 Key words: rotavirus/vaccine/immune response Immune Response to Rotavirus Polypeptides after Vaccination with Heterologous Rotavirus Vaccines
More informationAnalysis of Host Range Restriction Determinants in the Rabbit Model: Comparison of Homologous and Heterologous Rotavirus Infections
JOURNAL OF VIROLOGY, Mar. 1998, p. 2341 2351 Vol. 72, No. 3 0022-538X/98/$04.00 0 Copyright 1998, American Society for Microbiology Analysis of Host Range Restriction Determinants in the Rabbit Model:
More informationSupplementary Figures
Inhibition of Pulmonary Anti Bacterial Defense by IFN γ During Recovery from Influenza Infection By Keer Sun and Dennis W. Metzger Supplementary Figures d a Ly6G Percentage survival f 1 75 5 1 25 1 5 1
More informationThe Adaptive Immune Responses
The Adaptive Immune Responses The two arms of the immune responses are; 1) the cell mediated, and 2) the humoral responses. In this chapter we will discuss the two responses in detail and we will start
More informationThe Adaptive Immune Response. B-cells
The Adaptive Immune Response B-cells The innate immune system provides immediate protection. The adaptive response takes time to develop and is antigen specific. Activation of B and T lymphocytes Naive
More informationMedical Virology Immunology. Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University
Medical Virology Immunology Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University Human blood cells Phases of immune responses Microbe Naïve
More informationIOM Immunization Safety Review 11/12/2001. Immunological Competition and the Infant Immune Response to Vaccines
IOM Immunization Safety Review 11/12/2001 Immunological Competition and the Infant Immune Response to Vaccines Richard Insel University of Rochester Goals Neonatal and Infant Immune System Broad Effects
More informationVaccines against Rotavirus & Norovirus. Umesh D. Parashar CDC, Atlanta, GA
TM Vaccines against Rotavirus & Norovirus Umesh D. Parashar CDC, Atlanta, GA 1 Rotavirus is the Leading Cause Of Severe Diarrhea in Children
More informationPrevalence of norovirus GII-4 antibodies in Finnish children
Prevalence of norovirus GII- antibodies in Finnish children Kirsi Nurminen, Vesna Blazevic, Leena Huhti, Sirpa Räsänen, Tiia Koho, Vesa P. Hytönen, Timo Vesikari To cite this version: Kirsi Nurminen, Vesna
More informationSafety and Immunogenicity of a Parenterally Administered Rotavirus VP8 Subunit Vaccine in Healthy Adults
Safety and Immunogenicity of a Parenterally Administered Rotavirus VP8 Subunit Vaccine in Healthy Adults Stanley Cryz 1, Clayton Harro 2, Monica McNeal 3, Nicole Meyer 3, Barbara DeNearing 2, Alicia Cage
More informationSupporting Information
Supporting Information Sui et al..7/pnas.997 Pre-CLP CM9 LA9 SL Tat# Pol Vif % Tetramer + CD + CD + Vac+IL- +IL- Vac Fig. S. Frequencies of six different CD + CD + Mamu-A*-tetramer + cells were measured
More informationSubunit Rotavirus Vaccine Administered Parenterally to Rabbits Induces Active Protective Immunity
JOURNAL OF VIROLOGY, Nov. 1998, p. 9233 9246 Vol. 72, No. 11 0022-538X/98/$04.00 0 Copyright 1998, American Society for Microbiology. All Rights Reserved. Subunit Rotavirus Vaccine Administered Parenterally
More information24 26 January 2013, Hong Kong SAR, CHINA. TITLE from VIEW and SLIDE MASTER February 27, 2013
The first WHO integrated meeting on development and clinical trials of influenza vaccines that induce broadly protective and long-lasting immune responses 24 26 January 2013, Hong Kong SAR, CHINA 1 TITLE
More informationTransforming The Approach to Vaccines and Protein- Based Therapeutics. Alain Doucet, Ph.D. Manager, Process Development, Medicago
Transforming The Approach to Vaccines and Protein- Based Therapeutics Alain Doucet, Ph.D. Manager, Process Development, Medicago 2018-08-15 1 Medicago Overview 2 Novel Technologies 3 Robust Pipeline 2
More informationGastroenteritis and viral infections
Gastroenteritis and viral infections A Large number of viruses are found in the human gut; these include some that are associated with gastroenteritis Rotaviruses Adenoviruses 40/41 Caliciviruses Norwalk-like
More informationHeterotypic Protection and Induction of a Broad Heterotypic Neutralization Response by Rotavirus-Like Particles
JOURNAL OF VIROLOGY, June 1999, p. 4813 4822 Vol. 73, No. 6 0022-538X/99/$04.00 0 Copyright 1999, American Society for Microbiology. All Rights Reserved. Heterotypic Protection and Induction of a Broad
More informationVaccines in the Pipeline: Norovirus and Respiratory Syncytial Virus (RSV)
National Center for Immunization & Respiratory Diseases Vaccines in the Pipeline: Norovirus and Respiratory Syncytial Virus (RSV) Aron J. Hall, DVM, MSPH, Dipl ACVPM (RSV slides courtesy of Sue Gerber,
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationSusceptibility to Rotavirus Infections. Mary K. Estes, PhD Baylor College of Medicine
13 th International Rotavirus Symposium Session V: Advances in rotavirus immunology and virology Minsk, Belarus August 30, 2018 Susceptibility to Rotavirus Infections Mary K. Estes, PhD Baylor College
More informationScott Abrams, Ph.D. Professor of Oncology, x4375 Kuby Immunology SEVENTH EDITION
Scott Abrams, Ph.D. Professor of Oncology, x4375 scott.abrams@roswellpark.org Kuby Immunology SEVENTH EDITION CHAPTER 13 Effector Responses: Cell- and Antibody-Mediated Immunity Copyright 2013 by W. H.
More informationAGAINST VIRAL INFECTIONS. Identify the types of immunity involve in the mechanisms of protection against viral infections.
LECTURE: 02 Title: THE IMMUNOLOGICAL PROTECTIVE MECHANISMS AGAINST VIRAL INFECTIONS LEARNING OBJECTIVES: The student should be able to: Identify the types of immunity involve in the mechanisms of protection
More informationCorrelates of Vaccine-induced Immunity
Correlates of Vaccine-induced Immunity by 9/22/2010 10:42 AM Annecy Correlates 2010 Stanley A. Plotkin References: 1) Plotkin SA. 2001. Immunologic correlates of protection induced by vaccination. Ped.
More informationRotavirus Virus-Like Particles Administered Mucosally Induce Protective Immunity
JOURNAL OF VIROLOGY, Nov. 1997, p. 8707 8717 Vol. 71, No. 11 0022-538X/97/$04.00 0 Copyright 1997, American Society for Microbiology Rotavirus Virus-Like Particles Administered Mucosally Induce Protective
More informationROTAVIRUS VACCINES. Virology
ROTAVIRUS VACCINES Virology Rotavirus is a triple-layers viral particle belonging to the Reoviridae family. It contains 11 segments of double-stranded RNA, of which 6 are structural and 5 are non-structural
More informationPrinciple of the FluoroSpot assay. Anti-tag mab-green. Streptavidin-Red. Detection mab-tag. Detection mab-biotin. Analyte. Analyte.
FluoroSpot 1 The principle objective of the FluoroSpot assay is the simultaneous measurement of dual cytokine secretion at the single cell level. This is accomplished by using a mixture of monoclonal antibodies
More informationReplicating measles-shiv vaccine induces long term preservation of central memory CD4 cells in the gut of macaques challenged with SHIV89.
Replicating measles-shiv vaccine induces long term preservation of central memory CD4 cells in the gut of macaques challenged with SHIV89.6P Frédéric Tangy Viral Genomics and Vaccination Laboratory Measles
More informationRecombinant adeno-associated virus vectors induce functionally impaired transgene product specific CD8 + T cells in mice
Recombinant adeno-associated virus vectors induce functionally impaired transgene product specific CD8 + T cells in mice Shih-Wen Lin,, Marcio O. Lasaro, Hildegund C.J. Ertl J Clin Invest. 2007;117(12):3958-3970.
More information10th International Rotavirus Symposium Bangkok, Thailand
Rotavirus Host Range Restriction and Innate Immunity: Mechanisms of Vaccine Attenuation Harry Greenberg MD Stanford University 10th International Rotavirus Symposium Bangkok, Thailand 09/19/12 B dsrna
More informationFoot and Mouth Disease Control, A vaccine perspective. John Barlow, DVM PhD
Foot and Mouth Disease Control, A vaccine perspective John Barlow, DVM PhD john.barlow@uvm.edu May 28, 2015 Countries in which FMD was reported to the OIE between 1990 and 2002 Antigenic variation is common
More informationGOVX-B11: A Clade B HIV Vaccine for the Developed World
GeoVax Labs, Inc. 19 Lake Park Drive Suite 3 Atlanta, GA 3 (678) 384-72 GOVX-B11: A Clade B HIV Vaccine for the Developed World Executive summary: GOVX-B11 is a Clade B HIV vaccine targeted for use in
More informationSupplemental Table I.
Supplemental Table I Male / Mean ± SEM n Mean ± SEM n Body weight, g 29.2±0.4 17 29.7±0.5 17 Total cholesterol, mg/dl 534.0±30.8 17 561.6±26.1 17 HDL-cholesterol, mg/dl 9.6±0.8 17 10.1±0.7 17 Triglycerides,
More informationAs outlined under External contributions (see appendix 7.1), the group of Prof. Gröne at the
3 RESULTS As outlined under External contributions (see appendix 7.1), the group of Prof. Gröne at the DKFZ in Heidelberg (Dept. of Cellular and Molecular pathology) contributed to this work by performing
More informationReoviruses. Virion. Genome. Genes and proteins. Viruses and hosts. Diseases. Distinctive characteristics
Reoviruses Virion Genome Genes and proteins Viruses and hosts Diseases Distinctive characteristics Virion Naked icosahedral capsid (T=13), diameter 60-85 nm Capsid consists of two or three concentric protein
More informationEmerging Viruses. Part IIb Follow Up from Part I Vaccines and Inhibitors
Emerging Viruses Part IIb Follow Up from Part I Vaccines and Inhibitors Cellular Responses to Viral Invasion: Restriction Factors Cells fight viral infection using a series of restriction factors Restriction
More informationUpdate on Norovirus Vaccine Development
Update on Norovirus Vaccine Development Commander Dennis J. Faix, MD, MPH Military Population Health Naval Health Research Center San Diego, California, USA Commander Mark S. Riddle, MD, MPH&TM, DrPH Enteric
More informationLa risposta immune all infezione da virus ebola. Chiara Agrati, PhD
La risposta immune all infezione da virus ebola Chiara Agrati, PhD Pathogenetic mechanisms This virus infection is able to: - disable the immune system, preventing an effective protective immune response
More informationPD1-based DNA vaccine amplifies HIV-1 GAG-specific CD8 + T cells in mice
Research article PD1-based DNA vaccine amplifies HIV-1 GAG-specific CD8 + T cells in mice Jingying Zhou, 1 Allen K.L. Cheung, 1 Zhiwu Tan, 1 Haibo Wang, 1 Wenbo Yu, 1 Yanhua Du, 1 Yuanxi Kang, 1 Xiaofan
More informationAntigen Presentation and T Lymphocyte Activation. Abul K. Abbas UCSF. FOCiS
1 Antigen Presentation and T Lymphocyte Activation Abul K. Abbas UCSF FOCiS 2 Lecture outline Dendritic cells and antigen presentation The role of the MHC T cell activation Costimulation, the B7:CD28 family
More information% of live splenocytes. STAT5 deletion. (open shapes) % ROSA + % floxed
Supp. Figure 1. a 14 1 1 8 6 spleen cells (x1 6 ) 16 % of live splenocytes 5 4 3 1 % of live splenocytes 8 6 4 b 1 1 c % of CD11c + splenocytes (closed shapes) 8 6 4 8 6 4 % ROSA + (open shapes) % floxed
More informationSupporting Online Material for
www.sciencemag.org/cgi/content/full/1175194/dc1 Supporting Online Material for A Vital Role for Interleukin-21 in the Control of a Chronic Viral Infection John S. Yi, Ming Du, Allan J. Zajac* *To whom
More informationBBS 2711 Virology. Virus Vaccines
BBS 2711 Virology Virus Vaccines Dr Paul Young, Department of Microbiology & Parasitology. p.young@mailbox.uq.edu.au Virus Vaccines First vaccine developed by Jenner in late 1700's against smallpox virus
More informationTCR, MHC and coreceptors
Cooperation In Immune Responses Antigen processing how peptides get into MHC Antigen processing involves the intracellular proteolytic generation of MHC binding proteins Protein antigens may be processed
More informationVaccine Development for Respiratory Viruses in Children. James E. Gern MD Professor of Pediatrics and Medicine University of Wisconsin Madison USA
Vaccine Development for Respiratory Viruses in Children James E. Gern MD Professor of Pediatrics and Medicine University of Wisconsin Madison USA Speaker Disclosure In accordance with the policy of the
More informationAdaptive Immunity: Specific Defenses of the Host
17 Adaptive Immunity: Specific Defenses of the Host SLOs Differentiate between innate and adaptive immunity, and humoral and cellular immunity. Define antigen, epitope, and hapten. Explain the function
More informationSupplementary Figures
Supplementary Figures Supplementary Figure 1. NKT ligand-loaded tumour antigen-presenting B cell- and monocyte-based vaccine induces NKT, NK and CD8 T cell responses. (A) The cytokine profiles of liver
More informationProf. Ibtesam Kamel Afifi Professor of Medical Microbiology & Immunology
By Prof. Ibtesam Kamel Afifi Professor of Medical Microbiology & Immunology Lecture objectives: At the end of the lecture you should be able to: Enumerate features that characterize acquired immune response
More informationACTIVATION AND EFFECTOR FUNCTIONS OF CELL-MEDIATED IMMUNITY AND NK CELLS. Choompone Sakonwasun, MD (Hons), FRCPT
ACTIVATION AND EFFECTOR FUNCTIONS OF CELL-MEDIATED IMMUNITY AND NK CELLS Choompone Sakonwasun, MD (Hons), FRCPT Types of Adaptive Immunity Types of T Cell-mediated Immune Reactions CTLs = cytotoxic T lymphocytes
More informationHuman Immunodeficiency Virus
Human Immunodeficiency Virus Virion Genome Genes and proteins Viruses and hosts Diseases Distinctive characteristics Viruses and hosts Lentivirus from Latin lentis (slow), for slow progression of disease
More informationHCV Vaccine where do we stand? U. Spengler University of Bonn
HCV Vaccine where do we stand? U. Spengler University of Bonn THE IDEAL GOAL: The vaccine should induce sterilizing immunity A MORE REALISTIC GOAL: Prevention of chronic persistent infection Feinstone
More informationAdaptive Immune Response Day 2. The Adaptive Immune Response
Adaptive Immune Response Day 2 Chapter 16 The Adaptive Immune Response 1 The B cell receptor vs. the T cell receptor. The B cell receptor vs. the T cell receptor. 2 Which T cells have CD4 and which have
More informationCellular Immune response. Jianzhong Chen, Ph.D Institute of immunology, ZJU
Cellular Immune response Jianzhong Chen, Ph.D Institute of immunology, ZJU Concept of adaptive immune response T cell-mediated adaptive immune response I. Concept of immune response A collective and coordinated
More informationCover Page. The handle holds various files of this Leiden University dissertation
Cover Page The handle http://hdl.handle.net/1887/35908 holds various files of this Leiden University dissertation Author: Soema, Peter Title: Formulation of influenza T cell peptides : in search of a universal
More informationFMD VACCINE AND VACCINATION. Ahmad Al-Majali Dean, Faculty of Vet Medicine JUST Jordan
FMD VACCINE AND VACCINATION Ahmad Al-Majali Dean, Faculty of Vet Medicine JUST Jordan General Considerations on FMD vaccination The currently used FMD vaccines are killed virus preparations that are pure,
More informationExperience of Pentavalent Human-bovine Reassortant Rotavirus Vaccine Among Healthy Infants in Taiwan
ORIGINAL ARTICLE Experience of Pentavalent Human-bovine Reassortant Rotavirus Vaccine Among Healthy Infants in Taiwan Chien-Chih Chang, 1 Mei-Hwei Chang, 1 * Tzou-Yen Lin, 2 Hong-Chang Lee, 3 Wu-Shiun
More informationChapter 22: The Lymphatic System and Immunity
Bio40C schedule Lecture Immune system Lab Quiz 2 this week; bring a scantron! Study guide on my website (see lab assignments) Extra credit Critical thinking questions at end of chapters 5 pts/chapter Due
More informationAnti-infectious Immunity
Anti-infectious Immunity innate immunity barrier structures Secretory molecules Phagocytes NK cells Anatomical barriers 1. Skin and mucosa barrier 2.hemo-Spinal Fluid barrier 3. placental barrier Phagocytic
More informationUpdate on Pentavalent Human-Bovine Rotavirus Vaccine. Michelle Goveia, MD, MPH Medical Director MSD Vaccines India, September 2014
Update on Pentavalent Human-Bovine Rotavirus Vaccine Michelle Goveia, MD, MPH Medical Director MSD Vaccines India, September 2014 Outline Characteristics of RotaTeq and its worldwide use Recent data evaluating
More informationNeutrophils Macrophages CD4+ T-cells CD8+ T-cells B-cells None
Question 3 a) Cells present antigens on MHC I and MHC II molecules. The following table lists several different antigen types, and two different cell types that will present the antigen. For each box,
More informationCurrent Strategies in HIV-1 Vaccine Development Using Replication-Defective Adenovirus as a Case Study
Note: I have added some clarifying comments to the slides -- please click on Comments under View to see them. Current Strategies in HIV-1 Vaccine Development Using Replication-Defective Adenovirus as a
More informationPreventive and therapeutic HIV vaccines. Markus Bickel Infektiologikum Frankfurt
Preventive and therapeutic HIV vaccines Markus Bickel Infektiologikum Frankfurt No conflicts to declare Disclosures Background FAQ: by patients and colleagues Publication about promising results, especially
More informationThere are 2 major lines of defense: Non-specific (Innate Immunity) and. Specific. (Adaptive Immunity) Photo of macrophage cell
There are 2 major lines of defense: Non-specific (Innate Immunity) and Specific (Adaptive Immunity) Photo of macrophage cell Development of the Immune System ery pl neu mφ nk CD8 + CTL CD4 + thy TH1 mye
More informationCD4 + T-cell-inducing HIV vaccines may have an impact on viral control
CD4 + T-cell-inducing HIV vaccines may have an impact on viral control Clues from cytokines produced by CD4 + T-cells from HIVinfected patients and vaccinated seronegative volunteers Eva Van Braeckel 1,
More information1. Overview of Adaptive Immunity
Chapter 17A: Adaptive Immunity Part I 1. Overview of Adaptive Immunity 2. T and B Cell Production 3. Antigens & Antigen Presentation 4. Helper T cells 1. Overview of Adaptive Immunity The Nature of Adaptive
More informationNANO 243/CENG 207 Course Use Only
L4: Nanomedicine in Immunotherapy April 12, 2018 Branches of the Immune System Body has two main branches of immunity, innate and adaptive. Innate immunity is the first line of defense. Phagocytes such
More information4. Th1-related gene expression in infected versus mock-infected controls from Fig. 2 with gene annotation.
List of supplemental information 1. Graph of mouse weight loss during course of infection- Line graphs showing mouse weight data during course of infection days 1 to 10 post-infections (p.i.). 2. Graph
More informationDirect ex vivo characterization of human antigen-specific CD154 + CD4 + T cells Rapid antigen-reactive T cell enrichment (Rapid ARTE)
Direct ex vivo characterization of human antigen-specific CD154 + CD4 + T cells Rapid antigen-reactive T cell enrichment (Rapid ARTE) Introduction Workflow Antigen (ag)-specific T cells play a central
More informationTowards an HIV Cure Pre-Conference Symposium 20 & 21 July 2012
Towards an HIV Cure Pre-Conference Symposium 20 & 21 July 2012 Your logo Natural control of HIV infection is associated with an isotype switched IgG antibody response to HIV Gag antigens in patients with
More informationPutting it Together. Stephen Canfield Secondary Lymphoid System. Tonsil Anterior Cervical LN s
Putting it Together Stephen Canfield smc12@columbia.edu Secondary Lymphoid System Tonsil Anterior Cervical LN s Axillary LN s Mediastinal/Retroperitoneal LN s Thoracic Duct Appendix Spleen Inguinal LN
More informationImmune system and diabetes. Chairmen: J. Belkhadir (Morocco) N.M. Lalic (Serbia)
Immune system and diabetes Chairmen: J. Belkhadir (Morocco) N.M. Lalic (Serbia) Autoimmunity and prevention of type 1 diabetes R. Mallone (France) Autoimmunity and Prevention of Type 1 Diabetes Roberto
More informationCELL BIOLOGY - CLUTCH CH THE IMMUNE SYSTEM.
!! www.clutchprep.com CONCEPT: OVERVIEW OF HOST DEFENSES The human body contains three lines of against infectious agents (pathogens) 1. Mechanical and chemical boundaries (part of the innate immune system)
More informationD2 inhibits TLR2- initiated 12p40 transcription (-) TLR2 PGN MDP. MyD88 IRAK ECSIT TRAF6 NIK. Smallest unit of PGN muramyl dipeptide IKK.
D2 inhibits TLR2- initiated 12p40 transcription CARD CARD NOD2 LRR RICK/Rip2 NIK MDP TRAF6 PGN TLR2 MyD88 IRAK ECSIT (-) IKK Smallest unit of PGN muramyl dipeptide IκB NF-κB atanabe et al, 2004 NF-κB IL-12p40
More informationImproving global human health through norovirus virus-like particle manufacturing
Engineering Conferences International ECI Digital Archives Vaccine Technology VI Proceedings 6-15-2016 Improving global human health through norovirus virus-like particle manufacturing Scot Shepard Takeda
More informationSupplemental Figure 1
Supplemental Figure 1 1a 1c PD-1 MFI fold change 6 5 4 3 2 1 IL-1α IL-2 IL-4 IL-6 IL-1 IL-12 IL-13 IL-15 IL-17 IL-18 IL-21 IL-23 IFN-α Mut Human PD-1 promoter SBE-D 5 -GTCTG- -1.2kb SBE-P -CAGAC- -1.kb
More informationGenetically engineered structure-based vaccine for bluetongue disease
Vet. Ital., 40 (4), 594-600 Genetically engineered structure-based vaccine for bluetongue disease P. Roy Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine,
More information