Molecular Cartography:
|
|
- Sheena Sutton
- 6 years ago
- Views:
Transcription
1 Molecular Cartography: Moving Towards Combined Topographical and Chemical Imaging Using AFM and Mass Spectrometry Olga S. Ovchinnikova Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory
2 What is Molecular Cartography? 2 Managed by UT-Battelle
3 Why do molecular chemical imaging? PET Scan of human brain RAMAN Imaging of pharmaceuticals 3 Managed by UT-Battelle MALDI MS Imaging of tissue
4 Need for spatially resolved information 4 Managed by UT-Battelle
5 Overview Goal: Conceive, implement, study, and advance an imaging platform that can combine measurements of spatially resolved topographic and other physical features of a surface, under real world conditions, with precise molecular characterization of the chemical species at those spatial locations a.k.a., Molecular Cartography Concept: Combined atmospheric pressure proximal probe atomic force microscopy (AFM) and thermal desorption/ionization mass spectrometry (TD/I-MS) Experimental Progression: Implement and study proximal probe TD/I-MS with probes from millimeter to low micrometer size Enable TD/I-MS using traditional AFM platform with proximal probe capable of nanometer scale TD 5 Managed by UT-Battelle
6 Established and Emerging Atmospheric Pressure Surface Sampling/Ionization Techniques 6 Managed by UT-Battelle Addressing the Challenges to Enable Spatially Resolved Molecular Chemical Analysis of Interfaces Under Real World Conditions Van Berkel, Pasilis and Ovchinnikova, J. Mass Spectrom. 28, 43,
7 Auxiliary AP-Surface Methods: Sampling/Ionization Stirring/Heating Labile by Oscillatory Motion 7 Managed by UT-Battelle Atomic Molecular Molecules Reactive Separation Simplicity Topographic Liquid/Gas Jet Liquid Extractive Thermal Laser Force Limits 1 Å 1 nm 1 nm nm 1 μm 1 μm μm 1 mm 1 mm Jet Based Probing Extractive Probing Thermal Probing Laser Probing mm AFM Probing
8 Proximal Probe Thermal Desorption with a Secondary Ionization: Experimental Set-Up 3. Thermally desorbed species are ionized by interaction with ionic species from ESI or APCI source 2. Desorbed molecules are vacuumed into the sampling cone 4. Molecular I.D. (Full scan, SIM, or SRM) 1. Spatially resolved thermal desorption with heated probe
9 Proximal Probe TD/I-MS: Experimental Set-Up Heated probe tips of various sizes and shapes for different applications Probe adjustable up to 35 o C Computer controlled sample stage Camera to monitor probe to sample position (1) Array of Heated Probe Tips Width=.5mm 9 Managed by UT-Battelle (2) (3) (4) Width=.2mm Width=1.6mm Thickness=.7mm Width=2.4mm Thickness=.8mm
10 Detection Limits for Compounds from TLC Plates Using TD/I-MS The Detection Limits of Compounds for the TD/I-MS technique are highly dependent of the compound s Vapor Pressure TNT 1 ng Pharmaceuticals Explosives Herbicides Acetaminophen ng Dyes Compound detection limits were done using thin layer chromatography (TLC) plates Sudan Red 7B 25 ng 1 Managed by UT-Battelle
11 Proximal Probe TD/I-MS: Analytes Separated on and Sampled from a NP-HPTLC Plate D 5 (a ) (b ) Developed Plate Total Ion Current 1.6 mm chisel point TD probe Relative Intensity Managed by UT-Battelle 5 (M +H ) + m/z 152 m/z (M +N a) + m/z 23 m/z m/z Distance (mm) (M +H )+ m/z 195 (c ) (d ) (e ) Caffeine (M+H) + = m/z 195 APCI + Acetaminophen (M+H) + = m/z 152 APCI + Aspirin (3) (M+Na) + = m/z 23 ESI + Ovchinnikova and Van Berkel. Rapid Commun. Mass Spectrom., 21, 43, ESI APCI Compounds (active ingredients in Excedrin tablet) illustrate versatility of the technique Alternating scan to scan ionization by ESI and APCI Simultaneous detection of compounds with disparate ionization properties
12 Micrometer Scale TD/SI-MS 12 Managed by UT-Battelle
13 Micrometer Resolved Spot Sampling Relative Intensity Caffeine, APCI+ (M+H) + = m/z 195 Acetaminophen, APCI+ (M+H) + = m/z 152 Aspirin, ESI+ (M+Na) + = m/z 23 TIC Time (min) Excedrin Tablet Thermal desorption sampling holes ~25 µm in diameter Spot to spot chemical variation consistent with other chemical imaging (i.e. Raman) Ability to use different ionizations simultaneously
14 Resolution Testing of MicroTD Imaging Optical Image Chemical Image 5 um (a) (b) 1 mm 45 um 5 μm 5 μm 5 um (c) (d) 5 μm 2 um 25 μm 25μm 14 Managed by UT-Battelle Ovchinnikova, Kertesz and Van Berkel. Anal. Chem, 21, Submitted
15 Influence of Scan Direction on Resolution Optical Image Chemical Image 5 um (a) (b) 5 μm 5 μm 5 um 8 um 2 um 25 μm 25 μm (c) 8 um 5 um 5 um 2 um (d) Current resolution limit with current set-up is 5 μm 25 μm 15 Managed by UT-Battelle 25 μm
16 Nanometer Scale TD/SI-MS 16 Managed by UT-Battelle
17 Marriage of Mass Spectrometry with Microscopy Chemical specificity Physical properties and nanometer resolution + =? New Tool for Studying Systems at a Sub-micron Level!!! 17 Managed by UT-Battelle
18 What is Atomic Force Microscopy-AFM? AFM is a very high-resolution type of scanning probe microscope A microscale cantilever with a sharp tip (probe) at its end that is used to scan the specimen surface. When the tip is brought into proximity of a sample surface, forces between the tip and the sample lead to a deflection of the cantilever. 18 Managed by UT-Battelle
19 Local Nano-Thermal Analysis with an AFM Pre-Heating Scan Post-Heating Scan 4 C PMMA and SAN Polymer Blend 3 C 35 C 19 Managed by UT-Battelle Nano heating probes allow for rapid heating and cooling and precise temperature control.
20 Nanoscale Physical and Chemical Imaging via Proximal Probe Thermal Desorption/Mass Spectrometry 5. Combine AFM and MS information 4. Detect targeted molecules with a mass spectrometer 2. Image area with AFM to obtain topography and physical information 1. Locate area of interest on the tissue 3. Spatially Desorb areas of interest 2 Managed by UT-Battelle
21 Proximal Probe Thermal Desorption with a Secondary Ionization: Nanometer Scale Spot Sampling Atomic Force Microscope (AFM) cantilever used as thermal desorption proximal probe 5 Tip on surface 25 C m/z Tip removed from surface 1 μm 5 35 C m/z Tip on surface 35 C m/z Cantilever can be heated in a controlled fashion to 35 C at very rapid heating rates Surface is 1 μm caffeine thin film Caffeine (M+H) + = m/z 195 ESI + Caffeine only observed when the AFM probe is heating and engaged on the surface
22 Heated AFM tip lowered and held on caffeine thin film surface 1. Heater is turned on above surface 8 Relative Abundance Tip is engaged onto surface from 1 μm and heats the surface. Material desorbs Time (min) (SRM) of caffeine 195 m/z -> 138 m/z as tip is lowered onto surface and held there 22 Managed by UT-Battelle Single Reaction Monitoring (SRM) allows to only monitor the fragmentation transition of caffeine and therefore allows to significantly lower the noise
23 Size of Nano-thermal Holes Topography ~25 nm (a) (a) AFM topography of caffeine thin film surface after local thermal desorption with heated AFM tip (b) The holes are 25 nm wide x nm deep. This corresponds to approximately 8 Attomols. ~25 nm (b) 25 μm crater 2 μm crater ~ nm 2 μm crater 2 nm crater Region we are working in Attomoles of material sampled!
24 Proximal Probe Thermal Desorption with a Secondary Ionization: Nanometer Scale Spatial Resolution Array of holes created from locally heating surface Heater ON and lowered to surface Corresponding chronogram from SRM of caffeine (m/z ) 8 Hot tip Cold tip 1. Heater is turned on at 1 μm 2. Hot tip is slowly lowered on surface 3. Hot tip is held on surface for 3 s 4. Heater is turned off and tip is removed from surface 5. Tip moves to next point (2 s programmed delay between engagement of surface of subsequent holes Relative Intensity Heater OFF and retracted from surface Time (min)
25 Nanomechanical Measurements of Plant Cell Walls with AFM Map correlating Nanomechanical Properties of 5 nm x 5 nm area Nanomechanical studies of cells can reveal information about cell stiffness and structure by measuring the response of the cells to excitations supplied through the AFM tip. Optical Image of Cells in Poplar Stem Cross section in an AFM set-up AFM tip nm 2 μm Cell Wall dimensions 3 μm x 4 μm 5 x 5 nm area of top of cell wall Correlating 15 μm x 15 μm topography overlaid with AFM phase data of a plant cell wall
26 26 Managed by UT-Battelle Conclusion and Future Plans We have developed a platform for surface spot sampling chemical imaging via thermal desorption with a secondary ionization capable of millimeter to nanometer spatial resolution Spot or line sampling, or chemical imaging has been successfully demonstrated at different length scales Spot and line scans on HP-TLC plates at millimeter resolution Chemical imaging of printed inks on paper at micrometer resolution Spot sampling from thin-films on glass at nanometer resolution Combined TD spot sampling and topographic imaging of a surface at the 25 nm resolution Future Combined AFM and MS chemical imaging at higher resolution Image the distribution of small molecules in tissues and materials and correlate chemical information to physical properties
27 Acknowledgments Gary Van Berkel and Vilmos Kertesz of the Organic and Biological Mass Spectrometry Group, ORNL Maxim Nikiforov, Stephen Jesse, and Sergei Kalinin from the Center for Nanophase Material Sciences (CNMS) ORNL Michael Balogh from Waters Corporation for Beta-test TQD Instrument DOE Basic Energy Sciences 27 Managed by UT-Battelle For more information about TD/I-MS and the Mass Spectrometry Surface Sampling and Imaging Center
LECTURE 3. Ionization Techniques for Mass Spectrometry
LECTURE 3 Ionization Techniques for Mass Spectrometry Jack Henion, Ph.D. Emeritus Professor, Analytical Toxicology Cornell University Ithaca, NY 14850 Lecture 3, Page 1 Contents Electron ionization (EI)
More informationLOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016
LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry
More informationIon Source. Mass Analyzer. Detector. intensity. mass/charge
Proteomics Informatics Overview of spectrometry (Week 2) Ion Source Analyzer Detector Peptide Fragmentation Ion Source Analyzer 1 Fragmentation Analyzer 2 Detector b y Liquid Chromatography (LC)-MS/MS
More informationMethods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University
Methods in Mass Spectrometry Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Sample Engineering Chemistry Biology Life Science Medicine Industry IDF / Police Sample
More informationQuadrupole and Ion Trap Mass Analysers and an introduction to Resolution
Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution A simple definition of a Mass Spectrometer A Mass Spectrometer is an analytical instrument that can separate charged molecules according
More informationPolymer Additive Analysis by EI and APCI
Polymer Additive Analysis by EI and APCI Kate Yu, Eric Block LC/MS Waters Corporation Introduction to polymer additive analysis by LC/MS. Electron Ionization (EI) Advantages Classical spectra Library searchable
More informationSupporting Information
Supporting Information Wiley-VCH 2006 69451 Weinheim, Germany Tissue Imaging at Atmospheric Pressure using Desorption Electrospray Ionization (DESI) Mass Spectrometry Justin M. Wiseman, Demian R. Ifa,
More informationAn Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27
An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27 Top-down HRAM Bioanalysis of Native Proteins/Molecules Relative Abundance 100
More informationMass spectrometry imaging. What does MS imaging offer?
BMG 744 Mass spectrometry imaging Stephen Barnes, PhD With sincere acknowledgments to David Stella, PhD and Kyle A. Floyd, MS, former students in the Barnes Laboratory (2005 2012) and Kevin Schey, PhD,
More informationAn optical dosimeter for the selective detection of gaseous phosgene with ultra-low detection limit
Supporting information for An optical dosimeter for the selective detection of gaseous phosgene with ultra-low detection limit Alejandro P. Vargas, Francisco Gámez*, Javier Roales, Tània Lopes-Costa and
More informationIntroduction to LC/MS/MS
Trends in 2006 Introduction to LC/MS/MS By Crystal Holt, LC/MS Product Specialist, Varian Inc. Toxicology laboratories Increased use of LC/MS Excellent LD Cheaper (still expensive) Much more robust Solves
More informationAutomated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222
Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Joan Stevens, Ph.D.; Luke Roenneburg; Tim Hegeman; Kevin Fawcett
More informationFundamental Studies of Matrix-Assisted Laser Desorption/Ionization, Using Time-of-Flight Mass Spectrometry to Identify Biological Molecules
UCRL-ID-126246 Fundamental Studies of Matrix-Assisted Laser Desorption/Ionization, Using Time-of-Flight Mass Spectrometry to Identify Biological Molecules D. Eades, D. Wruck, H. Gregg November 11, 1996
More informationBiological Mass Spectrometry. April 30, 2014
Biological Mass Spectrometry April 30, 2014 Mass Spectrometry Has become the method of choice for precise protein and nucleic acid mass determination in a very wide mass range peptide and nucleotide sequencing
More informationDetection of oxygenated polycyclic aromatic hydrocarbons (oxy-pahs) in APCI mode with a single quadrupole mass spectrometer
APPLICATION NOTE 72819 Detection of oxygenated polycyclic aromatic hydrocarbons (oxy-pahs) in APCI mode with a single quadrupole mass spectrometer Author Maria Grübner; Thermo Fisher Scientific, Germering,
More informationAFM In Liquid: A High Sensitivity Study On Biological Membranes
University of Wollongong Research Online Faculty of Science - Papers (Archive) Faculty of Science, Medicine and Health 2006 AFM In Liquid: A High Sensitivity Study On Biological Membranes Michael J. Higgins
More informationMALDI-TOF. Introduction. Schematic and Theory of MALDI
MALDI-TOF Proteins and peptides have been characterized by high pressure liquid chromatography (HPLC) or SDS PAGE by generating peptide maps. These peptide maps have been used as fingerprints of protein
More informationThe vapour of imidazolium based ionic liquids: a mass spectrometry study
The vapour of imidazolium based ionic liquids: a mass spectrometry study A. Deyko, K. R. J. Lovelock*, P. Licence, R. G. Jones School of Chemistry The University of Nottingham University Park Nottingham
More informationSupporting Information. Evolution of atomically precise silver clusters to superlattices
Copyright WILEY-VCH Verlag GmbH & Co. KGaA, 69469 Weinheim, Germany, 2012. Supporting Information for Part. Part. Sys. Charact., DOI: 10.1002/ppsc.((please add manuscript number)) Evolution of atomically
More informationMass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010
Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Introduction, Ionization Methods Mass Analyzers, Ion Activation Methods
More informationSCS Mass Spectrometry Laboratory
SCS Mass Spectrometry Laboratory Contact Information Staff 31 Noyes Laboratory (8:00-5:00 M-F) 217-333-2545 http://scs.illinois.edu/massspec/ Furong Sun (frs@illinois.edu) Furong Sun Director Training
More informationIonization Methods. Neutral species Charged species. Removal/addition of electron(s) Removal/addition of proton(s)
Ionization Methods Neutral species Charged species Removal/addition of electron(s) M + e - (M +. )* + 2e - electron ionization Removal/addition of proton(s) M + (Matrix)-H MH + + (Matrix) - chemical ionization
More informationO O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions
Simplifying Qual/Quan Analysis in Discovery DMPK using UPLC and Xevo TQ MS Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION The determination of the drug metabolism
More informationMALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,
MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents
More informationSequence Identification And Spatial Distribution of Rat Brain Tryptic Peptides Using MALDI Mass Spectrometric Imaging
Sequence Identification And Spatial Distribution of Rat Brain Tryptic Peptides Using MALDI Mass Spectrometric Imaging AB SCIEX MALDI TOF/TOF* Systems Patrick Pribil AB SCIEX, Canada MALDI mass spectrometric
More informationFundamentals of Soft Ionization and MS Instrumentation
Fundamentals of Soft Ionization and MS Instrumentation Ana Varela Coelho varela@itqb.unl.pt Mass Spectrometry Lab Analytical Services Unit Index Mass spectrometers and its components Ionization methods:
More informationComparison of Full Scan MS2 and MS3 Linear Ion Trap Approaches for Quantitation of Vitamin D
Comparison of Full Scan MS2 and MS3 Linear Ion Trap Approaches for Quantitation of Vitamin D Julie A. Horner 1, Marta Kozak 1, Subodh Nimkar 1, and August A. Specht 1 1 Thermo Fisher Scientific, San Jose,
More informationCAMAG TLC-MS INTERFACE
CAMAG TLC-MS INTERFACE 93.1 249.2 40 30 97.1 20 10 250.2 0 200 400 m/z WORLD LEADER IN PLANAR-CHROMATOGRAPHY Identification and elucidation of unknown substances by hyphenation of TLC / HPTLC and MS The
More informationFuture Directions: Tissue and Cell Imaging Robert C. Murphy
LIPID MAPS Lipidomics Workshop April 19, 2009 www.lipidmaps.org m/z 806 (16:0a/22:6-PC) [M+H] + Future Directions: Tissue and Cell Imaging Robert C. Murphy 16:0/22:6 PC m/z 806.4 Department of Pharmacology
More informationTargeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur
Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS Kayleigh Arthur K.Arthur@lboro.ac.uk Introduction LC-MS is a highly used technique for untargeted profiling analyses
More informationRapid, Simple Impurity Characterization with the Xevo TQ Mass Spectrometer
Robert Plumb, Michael D. Jones, and Marian Twohig Waters Corporation, Milford, MA, USA INTRODUCTION The detection and characterization of impurities and degradation products of an active pharmaceutical
More informationMolecular pathology with desorption electrospray ionization (DESI) - where we are and where we're going
Molecular pathology with desorption electrospray ionization (DESI) - where we are and where we're going Dr. Emrys Jones Waters Users Meeting ASMS 2015 May 30th 2015 Waters Corporation 1 Definition: Seek
More informationINTRODUCTION TO MALDI IMAGING
INTRODUCTION TO MALDI IMAGING Marten F. Snel, Emmanuelle Claude, Thérèse McKenna, and James I. Langridge Waters Corporation, Manchester, UK INT RODUCTION The last few years have seen a rapid increase in
More informationCONTENT. i iv ix. SVKM s NMIMS, School of Pharmacy and Technology Management
CONTENT Chapter No. Title Page No. Abbreviations List of Figures List of Tables 1 Introduction 1 1.1 Practical aspects 4 1.2 Stability-indicating assay method (SIAM) 5 1.3 Regulatory aspects 6 1.4 Techniques
More informationAgilent s LC/MS Portfolio and Applications Examples
Agilent s LC/MS Portfolio and Applications Examples Sean Orlowicz LC/MS Product Specialist Agilent Technologies 31August Agilent s > 40 year heritage in mass spectrometry 1971, 5930A GC/MS 1971-2011 2005,
More informationThree Dimensional Mapping and Imaging of Neuropeptides and Lipids in Crustacean Brain
Three Dimensional Mapping and Imaging of Neuropeptides and Lipids in Crustacean Brain Using the 4800 MALDI TOF/TOF Analyzer Ruibing Chen and Lingjun Li School of Pharmacy and Department of Chemistry, University
More informationAgilent 6410 Triple Quadrupole LC/MS. Sensitivity, Reliability, Value
Agilent 64 Triple Quadrupole LC/MS Sensitivity, Reliability, Value Sensitivity, Reliability, Value Whether you quantitate drug metabolites, measure herbicide levels in food, or determine contaminant levels
More informationHiroya Hidaka *1), Masaki Takiwaki 2), Mine Yamashita 2), Shinya Otsuki 1), Kenji Kawasaki 3), Mitsutoshi Sugano 3) and Takayuki Honda 4)
Mild acid hydrolysis of sphingolipids yields lysosphingolipids: a matrix-assisted laser desorption and ionization time-of-flight mass spectrometry study Hiroya Hidaka *1), Masaki Takiwaki 2), Mine Yamashita
More informationNAS Workshop on the Industrialization of Biology
High throughput chemical characterization using mass spectrometry NAS Workshop on the Industrialization of Biology Jonathan V. Sweedler University of Illinois Urbana, IL USA May 2014 Funding: DOE, NSF
More informationMass Spectrometry. Actual Instrumentation
Mass Spectrometry Actual Instrumentation August 2017 See also http://www.uni-bielefeld.de/chemie/analytik/ms f additional infmation 1. MALDI TOF MASS SPECTROMETRY ON THE ULTRAFLEX 2 2. ESI MASS SPECTROMETRY
More informationMetabolomics: quantifying the phenotype
Metabolomics: quantifying the phenotype Metabolomics Promises Quantitative Phenotyping What can happen GENOME What appears to be happening Bioinformatics TRANSCRIPTOME What makes it happen PROTEOME Systems
More informationRaman spectroscopy methods for detecting and imaging supported lipid bilayers
Spectroscopy 24 (2010) 113 117 113 DOI 10.3233/SPE-2010-0426 IOS Press Raman spectroscopy methods for detecting and imaging supported lipid bilayers Claire S. Sweetenham and Ioan Notingher Nanoscience
More informationMass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications
- Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications Adapted from Mass Spectrometry in Biotechnology Gary Siuzdak,, Academic Press 1996 1 Introduction
More informationImaging Mass Microscope
Imaging Mass Microscope imscope C146-E220 Introducing the New Era of Imaging Mass Spectrometry Imaging mass spectrometry is a revolutionary new technology. The instrument is a combination of an optical
More informationAgilent 7700x ICP-MS
Agilent 7700x ICP-MS Metals analysis Mid ppt to low ppb detection range Option to detect in four different gas modes to gain optimum detection of multiple metals or interferences in a sample Autosampler
More informationAnalyzing Phenyl Ureas and Carbamates with HPLC/API-MS. Presented by Chen-Kai Meng, Ph.D. Applications Chemist
Analyzing Phenyl Ureas and Carbamates with HPLC/API-MS Presented by Chen-Kai Meng, Ph.D. Applications Chemist Outline Overview of ESI, APPI, and APCI sources Comparing ESI, APPI, and APCI results Identifying
More informationMatrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry
Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Time-of-Flight Mass Spectrometry. Basic principles An attractive feature of the time-of-flight (TOF) mass spectrometer is its
More informationNew Instruments and Services
New Instruments and Services http://planetorbitrap.com/orbitrap fusion Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion
More informationThe J105 SIMS. A New Instrument for 3-Dimensional Imaging and Analysis. Paul Blenkinsopp, Ionoptika Ltd
The J105 SIMS A New Instrument for 3-Dimensional Imaging and Analysis Paul Blenkinsopp, Ionoptika Ltd The J105 SIMS Why a new ToF Mass Spectrometer? The J105 ToF has been designed to allow us to separate
More informationMass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector
Mass Spectrometry MALDI-TOF ESI/MS/MS Mass spectrometer Basic components Ionization source Mass analyzer Detector 1 Principles of Mass Spectrometry Proteins are separated by mass to charge ratio (limit
More informationQuantitative Analysis of Vit D Metabolites in Human Plasma using Exactive System
Quantitative Analysis of Vit D Metabolites in Human Plasma using Exactive System Marta Kozak Clinical Research Applications Group Thermo Fisher Scientific San Jose CA Clinical Research use only, Not for
More informationMass Spectrometry Introduction
Mass Spectrometry Introduction Chem 744 Spring 2013 What MS is and is not MS is NOT a spectroscopic method. Molecules are not absorbing EM radiation MS is the generation, separation and characterization
More informationBruker Daltonics. autoflex III smartbeam. The Standard in MALDI-TOF Performance MALDI-TOF/TOF. think forward
Bruker Daltonics autoflex III smartbeam The Standard in MALDI-TOF Performance think forward MALDI-TOF/TOF Designed for a Routine High Level of Performance The autoflex III smartbeam brings the power of
More informationMS-IMS (MALDI-IMAGING)?
MS-IMS (MALDI-IMAGING)? Protein Chemistry/Proteomics and Peptide Synthesis and Array Unit Biomedicum Helsinki and Haartman Institute E-Mail: marc.baumann@helsinki.fi (http://research.med.helsinki.fi/corefacilities/proteinchem)
More informationChemical and topographical 3D surface profiling using atmospheric pressure LDI and MALDI MS imaging
Chemical and topographical 3D surface profiling using atmospheric pressure LDI and MALDI MS imaging Mario Kompauer, Sven Heiles & Bernhard Spengler Spengler Lab (University of Giessen) Abstract Mass spectrometry
More informationSmall Molecule Science: Experimental designs for achieving ultra trace analysis
Small Molecule Science: Experimental designs for achieving ultra trace analysis Michael P. Balogh Principal scientist Waters Corporation 211 Waters Corporation 1 www.cosmoscience.org The Society for Small
More informationUsing Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies.
Using Software Tools to Improve the Detection of Impurities Application Note Christine Miller Introduction The analysis of raw materials and finished products for or impurities presents a challenge in
More informationEdgar Naegele. Abstract
Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast LC/TOF mass spectrometry Application ote Edgar aegele Abstract A recent trend in
More informationMALDI-IMS (MATRIX-ASSISTED LASER DESORPTION/IONIZATION
MALDI-IMS (MATRIX-ASSISTED LASER DESORPTION/IONIZATION IMAGING MASS SPECTROMETER) IN TISSUE STUDY YANXIAN CHEN MARCH 8 TH, WEDNESDAY. SEMINAR FOCUSING ON What is MALDI imaging mass spectrometer? How does
More informationChemical Surface Transformation 1
Chemical Surface Transformation 1 Chemical reactions at Si H surfaces (inorganic and organic) can generate very thin films (sub nm thickness up to µm): inorganic layer formation by: thermal conversion:
More informationChapter 12: Mass Spectrometry: molecular weight of the sample
Structure Determination: hapter 12: Mass Spectrometry- molecular weight of the sample; formula hapter 12: Infrared Spectroscopy- indicated which functional groups are present hapter 13: Nuclear Magnetic
More informationAuthors. Abstract. Introduction. Environmental
Determination of Ultratrace Amitrol in Water Samples by in situ Derivatization-Solid Phase Extraction-Liquid Chromatography-Mass Selective Detector Application Environmental Authors Gerd Vanhoenacker,
More information2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry
Dr. Sanjeeva Srivastava 1. Fundamental of Mass Spectrometry Role of MS and basic concepts 2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry 2 1 MS basic concepts Mass spectrometry - technique
More informationIdentification of Steroids in Water by Ion Trap LC/MS/MS Application
Identification of Steroids in Water by Ion Trap LC/MS/MS Application Environmental Author Paul Zavitsanos and Christine Miller Agilent Technologies, Inc. Centerville Road Wilmington, DE 9- USA Abstract
More informationMASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010
MASS SPECTROMETRY BASED METABOLOMICS Pavel Aronov ABRF2010 Metabolomics Research Group March 21, 2010 Types of Experiments in Metabolomics targeted non targeted Number of analyzed metabolites is limited
More informationShotgun Proteomics MS/MS. Protein Mixture. proteolysis. Peptide Mixture. Time. Abundance. Abundance. m/z. Abundance. m/z 2. Abundance.
Abundance Abundance Abundance Abundance Abundance Shotgun Proteomics Protein Mixture 1 2 3 MS/MS proteolysis m/z 2 3 Time µlc m/z MS 1 m/z Peptide Mixture m/z Block Diagram of a Mass Spectrometer Sample
More informationProtein Analysis using Electrospray Ionization Mass Spectroscopy *
OpenStax-CNX module: m38341 1 Protein Analysis using Electrospray Ionization Mass Spectroscopy * Wilhelm Kienast Andrew R. Barron This work is produced by OpenStax-CNX and licensed under the Creative Commons
More informationCharacterization of Noncovalent Complexes of Polyelectrolytes by Mass Spectrometry
The University of Akron IdeaExchange@UAkron Honors Research Projects The Dr. Gary B. and Pamela S. Williams Honors College Spring 2015 Characterization of Noncovalent Complexes of Polyelectrolytes by Mass
More informationSupplementary Figure 1. Sample preparation schematic. First (Stage I), square islands of MoO 3 are prepared by either photolithography followed by
Supplementary Figure 1. Sample preparation schematic. First (Stage I), square islands of MoO 3 are prepared by either photolithography followed by thermal evaporation and liftoff or by a process where
More informationAnalysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF
Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF Application Note 219 Joan Stevens, PhD; Luke Roenneburg; Kevin Fawcett (Gilson,
More informationImaging MS: Present and Future of Multimodal Studies
1 Workshop Report Imaging MS: Present and Future of Multimodal Studies Wednesday 8th of June, 5:45-7:00, Room 304, level 3 Presiding: Vilmos Kertesz Oak Ridge National Laboratory, Oak Ridge, TN, U.S.A.
More informationQuantitative Analysis of Drugs of Abuse in Urine using UHPLC Coupled to Accurate Mass AxION 2 TOF Mass Spectrometer
application Note Liquid Chromatography/ Mass Spectrometry Authors Sharanya Reddy Blas Cerda PerkinElmer, Inc. Shelton, CT USA Quantitative Analysis of Drugs of Abuse in Urine using UHPLC Coupled to Accurate
More informationSupplementary Figure S1 Black silicon and dragonfly wing nanotopography.
Supplementary Figure S1 Black silicon and dragonfly wing nanotopography. Representative low-magnification scanning electron micrographs of a) Black silicon (bsi) and b) Diplacodes bipunctata dragonfly
More information1. Sample Introduction to MS Systems:
MS Overview: 9.10.08 1. Sample Introduction to MS Systems:...2 1.1. Chromatography Interfaces:...3 1.2. Electron impact: Used mainly in Protein MS hard ionization source...4 1.3. Electrospray Ioniztion:
More informationReversible Formation of Ag 44 from Selenolates
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2014 Supporting information for the paper Reversible Formation of Ag 44 from Selenolates Indranath
More informationAutomated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System
Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System Keywords Introduction Application Note PHA0413 High Pressure Liquid Chromatography
More informationOMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT
OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT PA/PH/OMCL (10) 86 2R QUALIFICATION OF EQUIPMENT ANNEX 7: QUALIFICATION OF MASS SPECTROMETERS Full document title and reference Document
More informationThe time has come: SINGLE CELL western blotting. Parrinello Natalia TJC
The time has come: SINGLE CELL western blotting Parrinello Natalia TJC 08.07.2014 To fully understand diverse and often rare behaviors in complex cell populations, researchers need analytical tools that:
More informationApplication of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices
Application ote #LCMS-2 esquire series Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices Introduction The simple monitoring
More informationIon Suppression Minimized in LC/MSD
Welcome to our E-Seminar: Ion Suppression Minimized in LC/MSD Outline What is ion/signal suppression and why Approaches to minimize suppression Different ionization process ESI source design Fruit and
More informationSolution range. Superior accuracy in surface interaction analysis
Solution range Superior accuracy in surface interaction analysis [ Q-SENSE ] Explore the nanoscale world with Q-Sense Based on established and powerful quartz crystal microbalance with dissipation (QCM-D)
More informationMASS SPECTROMETRY IN METABOLOMICS
For personal use only. Please do not reuse or reproduce without the author s permission MASS SPECTRMETRY IN METABLMICS Pavel Aronov Stanford Mass Spectrometry Users Meeting August 21, 2008 rigin of Metabolomics
More informationBioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS
Bioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS Jenny Chen, Hongxia Wang, Zhiqi Hao, Patrick Bennett, and Greg Kilby Thermo Fisher
More informationTHE continuous growth in data storage is met by the
IEEE TRANSACTIONS ON MAGNETICS, VOL. 52, NO. 2, FEBRUARY 2016 1100105 Optical and Thermal Behaviors of Plasmonic Bowtie Aperture and Its NSOM Characterization for Heat-Assisted Magnetic Recording Xianfan
More informationProtein directed assembly of lipids
Protein directed assembly of lipids D. Nordin, O. Yarkoni, L. Donlon, N. Savinykh, and D.J. Frankel SUPPLEMENTARY MATERIAL Materials and Methods Supported bilayer preparation 1,2-dioleoyl-sn-glycero-3-phosphocholine
More informationNew Solvent Grade Targeted for Trace Analysis by UHPLC-MS
New Solvent Grade Targeted for Trace Analysis by UHPLC-MS Subhra Bhattacharya, Deva H. Puranam, and Stephen C. Roemer Thermo Fisher Scientific Fisher Chemical, One Reagent Lane, Fair Lawn, NJ Material
More informationWhat You Can t See Can Hurt You. How MS/MS Specificity Can Bite Your Backside
What You Can t See Can Hurt You How MS/MS Specificity Can Bite Your Backside Johan van den Heever, Tom Thompson, and Don Noot Agri-Food Laboratories Branch Advances in Trace rganic Residue Analysis early
More informationRobust and Fast Analysis of Tobacco-Specific Nitrosamines by LC-MS/MS
Application Note 242 Robust and Fast Analysis of Tobacco-Specific Nitrosamines by LC-MS/MS INTRODUCTION Tobacco-specific nitrosamines (TSNA) are a group of carcinogens found only in tobacco products. They
More informationPTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System
Application Note LC/MS PTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System Purpose This application note describes an automated workflow
More informationSingle Quadrupole Compact Mass Spectrometer for Liquid Sample
Single Quadrupole Compact Mass Spectrometer for Liquid Sample YL INSTRUMENT CO., LTD. YL9900 MS quickly provides essential information and improves the chemist's workflow. Mordern organic labs have become
More informationLipid Bilayer Based Binding Surfaces for Nucleic Acids
Lipid Bilayer Based Binding Surfaces for Nucleic Acids Caitlin Kreutz Major: Biology Mentor: Emin Oroudjev Faculty Advisor: Helen Hansma Funding By: National Institutes of Health July 28 th, 2005 Main
More informationMore structural information with MS n
PRODUCT SPECIFICATIONS The LTQ XL linear ion trap mass spectrometer More structural information with MS n The LTQ XL linear ion trap mass spectrometer delivers more structural information faster and with
More informationMass spectrometry Technologies in Lipid chemistry
Mass spectrometry Technologies in Lipid chemistry Rabah Soliymani University Of Helsinki Protein Chemistry Unit Biomedicum Helsinki Rabah.soliymani@helsinki.fi Complex_&_dynamic_mixtures (few copies to
More informationMass Spectrometry Infrastructure
Mass Spectrometry Infrastructure Todd Williams, Ph.D. Director KU Mass Spectrometry and Analytical Proteomics Laboratory Mass Spectrometry Lab B025 Malott Hall Mission The Mass Spectrometry and analytical
More informationAnalysis of Pesticides (II) Metribuzin & their metabolites in Rice Jun Yonekubo, Nihon Waters, JAPAN
Waters Alliance LC/MS System Analytical Conditions Chromatograms Mass Spectra Calibration Quantitation Analysis of Pesticides (II) Metribuzin & their metabolites in Rice Jun Yonekubo,, JAPAN Key Words
More informationQuantitative Mass Spectrometry Imaging of Molecules in Biological Systems.
1 Quantitative Mass Spectrometry Imaging of Molecules in Biological Systems Ingela Lanekoff 1 * and Julia Laskin 2 * 1) Dept. of Chemistry BMC, Uppsala University, Box 599, 751 24 Uppsala, Sweden 2) Physical
More informationSolving practical problems. Maria Kuhtinskaja
Solving practical problems Maria Kuhtinskaja What does a mass spectrometer do? It measures mass better than any other technique. It can give information about chemical structures. What are mass measurements
More informationComprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids
PO-CON1576E Comprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids HPLC 2015 PSB-MULTI-06 Yoshiyuki WATABE, Tetsuo IIDA, Daisuke NAKAYAMA, Kanya TSUJII, Saki
More informationDART MSI of drugs of abuse in hair
DART MSI of drugs of abuse in hair Wilco Duvivier, Teris van Beek, Michel Nielen October 5, 2017 Forensic hair evidence Forensically interesting: Many compounds accumulate in hair Prolonged detectability
More informationStrengthening Results. Advanced Chromatography with Mass Detection
Strengthening Results Advanced Chromatography with Mass Detection UV Detection Why Not Use UV For Everything? Identification Difficult selectivity in complex matrices Sensitivity Need sufficient molar
More information