Determination of Carbohydrates in Beverages and Milk Products by HPAE-PAD and Capillary HPAE-PAD
|
|
- Brent Preston
- 6 years ago
- Views:
Transcription
1 Determination of Carbohydrates in Beverages and Milk Products by HPAE-PAD and Capillary HPAE-PAD Terri Christison, Monika Verma, Aaron Kettle, Carl Fisher, Linda Lopez; Thermo Fisher Scientific, Sunnyvale, CA, USA
2 Overview Carbohydrates in beverage samples and milk products were directly determined using capillary format high performance anion-exchange with pulsed amperometric detection (HPAE-PAD) on a capillary reagent-free, high-pressure system., FIGURE. Conven This method eliminates the costly and labor-intensive derivitization used in other methods. Lactose and lactulose determinations are also demonstrated on the new µm resin particle, high-capacity Thermo Scientific Dionex CarboPac SA-µm mm column optimized for fast separations of mono- and di- saccharide sugars. Conven electrod Introduction Monosaccharide and disaccharide determinations are important to the food industry to ensure product formulation and product quality and to report ingredients to immune-and allergy-sensitive individuals. Because carbohydrates are poor chromophores, chemical derivitization is needed for absorption. However, derivitization is costly, labor-intensive and may cause changes in molecular configuration. High Performance Anion-Exchange chromatography with Pulsed Amperometric Detection (HPAE-PAD) is a proven sensitive method to directly and selectively determine carbohydrates. In HPAE-PAD, carbohydrates are ionized in strong base and separated by anion-exchange chromatography. The carbohydrates are detected by PAD with a gold working electrode using a four-potential waveform selective and sensitive for carbohydrates. This sensitivity allows carbohydrate analysis down to pmole concentrations or when the samples are limited. This sensitivity is moderated in beverage samples which contain g/l concentrations by minimizing the flow path combined with moderate dilution. Here we demonstrate the determinations of the different sugars used to sweeten beverages and the fast and easy determination of lactose and lactulose in milk products. This work further demonstrates the versatility of HPAE-PAD, easily optimized for low or high carbohydrate concentrations, and separations on different carbohydrate columns, and High-Pressure IC (HPIC ) and capillary HPIC systems. Experimental Results Sugars in Beverag Beverages are gen available in the man sweetening. The m or lowest cost. The Corn syrup, which i increased sweetnes hydrolyzed to gluco which can cause st Figure show the s µl sample separate FIGURE. Six c Sample Preparation The beverage samples were prepared by dilution and filtration, as appropriate. The milk products ( g/ ml water) were treated with µl each of Carrez I (potassium hexacyanoferrate(iii)) and Carrez II (zinc sulfate) solutions for deproteinization according to AOAC Method 98. and as described in AN 8. The samples were mixed, diluted to ml, centrifuged, and the supernatant filtered and treated with a Thermo Scientific Dionex OnGuard IIA sample preparation to remove anionic contaminants and neutralize the sample. Method Thermo Scientific Dionex HPIC Ion Chromatography systems used: Thermo Scientific Dionex ICS-+ ion chromatography (IC) system, standard and capillary format Dionex ICS-+ IC modules: EG Eluent Generator, DC Detector Chromatography, and DP Dual Pump Thermo Scientific Dionex ICS- Capillary HPIC system Detection: Figures to 7 sho typical capillary ga characteristic of na indicating high fruc of product sweeten fructose and gluco Electrochemical Detector and Electrochemical cell Gold on PTFE Disposable Electrode and ph,-ag/agci Reference Electrode FIGURE. Nativ PTFE Gaskets:.,., or. Autosampler: Thermo Scientific Dionex AS-AP Autosampler Software: Thermo Scientific Dionex Chromeleon Chromatography Data System Determination of Carbohydrates in Beverages and Milk Products by HPAE-PAD and Capillary HPAE-PAD Figure shows the instrument flow diagram for a capillary HPAE-PAD system. The + 7
3 Electrochemical Detector and Electrochemical cell FIGURE. Nativ Gold on PTFE Disposable Electrode and ph,-ag/agci Reference Electrode PTFE Gaskets:.,., or. Autosampler: Thermo Scientific Dionex AS-AP Autosampler 7 Software: Thermo Scientific Dionex Chromeleon Chromatography Data System Figure shows the instrument flow diagram for a capillary HPAE-PAD system. The Dionex ICS-+ system configured for mm or mm format columns has the same flow path but without the CRD and suppressor bypass modules. Glucos Figures and show four-potential waveform for detection of carbohydrates (Figure ) using a gold disposable working electrode (Figure ). This waveform is optimized to create a stable gold oxide layer resulting in highly reproducible responses. FIGURE. Flow diagram for a capillary HPAE-PAD system. H Data & System Management High-Pressure Non-Metallic Capillary Pump CRD Bypass Eluent Generator (OH or H+) FIGURE. HFC in Suppressor Bypass Regen flow in back CR-TC % % Separation Column Waste 9 Degas ED Sample Inject (Autosampler) FIGURE. Four Potential Waveform for carbohydrate determinations. Potential (V vs. Ag/AgCl) ohydrates in Beverages and Milk Products by. Time (s) Start. -. End Potential (V) Integration aron Kettle, Carl Fisher, Linda Lopez; Thermo Fisher Scientific, Sun determined using ometric detection Time (Seconds) FIGURE. Conventional and disposable gold working electrodes. FIGURE 7. Cane Working electrode ed in other Sucros 7 ew µm resin -µm mm gars. Conventional electrode Disposable electrode ood industry to to immune-and hores, chemical abor-intensive Results ometric Detection ermine and separated y PAD with a gold e for Sugars in Beverages Beverages are generally sweetened with the most available and the lowest cost sugar Figure 8 shows th available in the manufacturing region. Native sugars from fruit may be used for sensitivity and req sweetening. The manufacturer often selects the sugar for its product based on availability sweetening. or lowest cost. These sugars are sourced from either corn, Thermo sugar Scientifi cane or sugar beets. c Poster Note PN79_HPLC E_/S Corn syrup, which is primarily glucose, is enzymatically hydrolyzed to create fructose for Au Contact pad
4 food industry to s to immune-and phores, chemical labor-intensive Results erometric Detection etermine e and separated by PAD with a gold ve for mole erated in beverage combined with Sugars in Beverages Beverages are generally sweetened with the most available and the lowest cost sugar available in the manufacturing region. Native sugars from fruit may be used for sweetening. The manufacturer often selects the sugar for its product based on availability or lowest cost. These sugars are sourced from either corn, sugar cane or sugar beets. Corn syrup, which is primarily glucose, is enzymatically hydrolyzed to create fructose for increased sweetness. Beet sugar and cane sugar, which are sucrose, are partially hydrolyzed to glucose and fructose to increase sweetness and to minimize crystallization which can cause storage problems. o sweeten e in milk products. mized for low or ydrate columns, Au Contact pad Figure show the separation of carbohdyrates using capillary format HPAE-PAD,. µl sample separated on. mm i.d. columns at 8 µl/min flow rates. Figure 8 shows sensitivity and re sweetening. FIGURE 8. HFC FIGURE. Six carbohydrate standard. Column: Dionex CarboPac PA with guard,. mm Eluent Source: Thermo Scientific Dionex EGCKOH capillary Eluent: mm KOH Flow Rate:.8 ml/min Inj. Volume:. µl Column Temp.: C Detection: PAD, Au disposable, -Potential Gasket:. PTFE Samples: µm Mixed Standard ropriate. Carrez I for in AN 8. The ant filtered and aration to Peaks: tem, standard and. Fucose. Galactosamine. Glucosamine. Galactose.. Mannose Figures to 7 show the analysis of beverage samples using capillary HPAE-PAD with a typical capillary gasket of. thick. In Figure, the sample had a mixture of sugars, characteristic of native sugars. In Figure, only glucose and fructose are present indicating high fructose corn syrup (HFC) for sweetening. Figure 7 shows a typical profile of product sweetened with cane sugar (predominantly sucrose with equal parts of fructose and glucose). ector Figure 9 shows t SA-µm colum ( mm) and the s facilitates the sep FIGURE 9. Lacto C B Electrode FIGURE. Native sugars in apple cider. A Data System 7 Column: system. The s has the same drates (Figure ) optimized to ses. Dionex CarboPac PA with guard,. mm Eluent: mm KOH Flow Rate:.8 ml/min Inj. Volume:. µl Column Temp.: C Detection: PAD, Au disposable, -Potential Gasket:. PTFE Sample Prep.: -fold dilution Peaks: Data & System Management FIGURE. HFC in a carbonated beverage. Determination of Carbohydrates in Beverages and Milk Products by HPAE-PAD and Capillary HPAE-PAD %,. Void Volume. Galactose.. Mannose.. -- µm. Conclusio Capillary HPA without costly Modifying the analyzing high An RFIC syste thereby elimin Fast determin a simply 8-min Reference. Thermo Fish and Disacch
5 FIGURE. Native sugars in apple cider. 7 Column: Dionex CarboPac PA with guard,. mm Eluent: mm KOH Flow Rate:.8 ml/min Inj. Volume:. µl Column Temp.: C Detection: PAD, Au disposable, -Potential Gasket:. PTFE Sample Prep.: -fold dilution Peaks:. Void Volume -- µm. Galactose... Mannose.. FIGURE. HFC in a carbonated beverage. %, % 9 Column: Dionex CarboPac PA with guard,. mm Eluent: mm KOH Flow Rate:.8 ml/min Inj. Volume:. µl Column Temp.: C Detection: PAD, Au disposable, -Potential Gasket:. PTFE Sample Prep.: -fold dilution, degas Peaks:. 98 µm. 9 Thermo Scientifi c Poster Note PN79_HPLC E_/S
6 FIGURE 7. Cane sugar in coconut water flavored beverage 7 Column: Dionex CarboPac PA with guard,. mm Eluent: mm KOH Flow Rate:.8 ml/min Inj. Volume:. µl Column Temp.: C Detection: PAD, Au disposable, -Potential Gasket:. PTFE Sample Prep.: -fold dilution Peaks:. Void Volume -- µm. Galactose.. 7. Figure 8 shows that using a. gasket versus the. capillary gasket reduces sensitivity and requires less dilution. The resulting analysis suggest the use of HFC for sweetening. FIGURE 8. HFC in a carbonated beverage --. Gasket. %, % Column: Dionex CarboPac PA with guard,. mm Eluent: mm KOH Flow Rate:.8 ml/min Inj. Volume:. µl Column Temp.: C Detection: PAD, Au on PTFE disposable, Four-Potential Carbohydrate waveform Gasket:. PTFE Sample Prep.: -fold dilution, degas Peaks: mg/l % Ratio. Void Volume Figure 9 shows the 8 min separation of lactose and lactulose on the Dionex CarboPac SA-µm column using only mm KOH. This method utilizes the standardbore format ( mm) and the standard. thickness gasket. A high-pressure capable system facilitates the separation. Determination of Carbohydrates in Beverages and Milk Products by HPAE-PAD and Capillary HPAE-PAD
7 FIGURE 9. Lactose and lactulose in raw unpasteurized milk. C B A Instrument: Dionex ICS- + HPIC system Column: Dionex CarboPac SA-µm and guard, mm Eluent Source: Dionex EGC KOH Eluent: mm KOH Flow Rate:. ml/min Inj. Volume: µl Column Temp.: C Detection: PAD, Au on PTFE disposable, Four-potential Gasket:. thick PTFE Ref. Electrode: ph-ag/agcl Sample Prep.: Carrez digestion, centrifuge, filter, Dionex OnGuard IIA Sample: A: -fold diluted raw, unpasteurized milk B: Sample A +. mg/l lactulose C:. mg/l carbohydrate standard Peaks: A B mg/l. Galactose Lactose Lactulose --.8 Conclusion Capillary HPAE-PAD and HPAE-PAD are direct, selective and sensitive methods without costly and labor intensive derivitization Modifying the gasket type affects the sensitivity which can be advantageous when analyzing high carbohydrate concentrations An RFIC system with electrolytically generated eluent requires only adding water, thereby eliminating eluent preparation, increasing reliability, stability, and ease-of-use Fast determinations of lactose and lactulose in milk products were demonstrated using a simply 8-min isocratic separation on the Dionex CarboPac SA column References. Thermo Fisher Scientific Technical Note : Determinations of Monosaccharides and Disaccharides in Beverages by Capillary HPAE-PAD. Sunnyvale, CA,. [Online] ication%&%technical%notes/chromatography/ion%chromatography/ic% and%rfic%systems/tn--determination-monosaccharides- Disaccharides-Beverages-TN7-E.pdf (accessed April 7, ).. Thermo Fisher Scientific Technical Note : Fast Determinations of Lactose and Lactulose in Milk Products Using HPAE-PAD. Sunnyvale, CA, [Online] duct%manuals%&%specifications/chromatography/ion%chromatography/ TN_789_Rev.pdf (accessed April 7, ). 8 Thermo Fisher Scientifi c Inc. All rights reserved. ISO is a trademark of the International Standards Organization. All other trademarks are the property of Thermo Fisher Scientifi c Inc. and its subsidiaries. This information is presented as an example of the capabilities of Thermo Fisher Scientifi c Inc. products. It is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. Specifi cations, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. Africa + Australia Austria Belgium + 7 Brazil + 7 Canada China (free call domestic) 8 Denmark + 7 Europe-Other + Finland France Germany +9 8 India Italy Japan Korea +8 8 Latin America Middle East + Netherlands New Zealand Norway Thermo Fisher Scientifi c, Sunnyvale, CA USA is ISO 9:8 Certifi ed. Russia/CIS + Singapore Sweden Switzerland Taiwan UK/Ireland + USA PN79_E /S
Carl Fisher, Terri Christison, Hua Yang, Monika Verma, and Linda Lopez Thermo Fisher Scientific, Sunnyvale, CA, USA
Fast Determination of Lactose and Lactulose in Dairy Products Using a 4 μm Particle Column and High-Performance Anion-Exchange Chromatography with Carl Fisher, Terri Christison, Hua Yang, Monika Verma,
More informationDetermination of Carbohydrates in Kombucha Using HPAE-PAD
Determination of Carbohydrates in Kombucha Using HPAE-PAD Beibei Huang, Jingli Hu, and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA USA Application Note 1152 Key Words Glucose, Fructose, Sucrose,
More informationDetection and Quantification of Inorganic Arsenic in Fruit Juices by Capillary Ion Chromatography with Suppressed Conductivity Detection
Detection and Quantification of Inorganic Arsenic in Fruit Juices by Capillary Ion Chromatography with Suppressed Conductivity Detection Hua Yang, Linda Lopez Thermo Fisher Scientific, Sunnyvale, CA, USA
More informationThermo Scientific Dionex CarboPac MA1 Column
CHROMATOGRAPHY Thermo Scientific Dionex CarboPac MA Column Product Specifications HPLC columns for the analysis of weakly ionizable carbohydrates. Simple methods with pulsed amperometric detection Reduced
More informationMercury Speciation Determinations in Asian Dietary Supplements
Mercury Speciation Determinations in Asian Dietary Supplements Terri Christison, Deepali Mohindra, Frank Hoefler, and Linda Lopez, Thermo Fisher Scientific, Sunnyvale, California, USA Overview Purpose:
More informationA HPAE-PAD Chromatographic Assay for Carbohydrates in Urine as a Measure of Intestinal Permeability
A HPAE-PAD Chromatographic Assay for Carbohydrates in Urine as a Measure of Intestinal Permeability Deanna Hurum and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Overview Purpose: A high-performance
More informationThe Determination of Sugars in Molasses by High-Performance Anion Exchange with Pulsed Amperometric Detection
Application Note 9 The Determination of Sugars in Molasses by High-Performance Anion Exchange with Pulsed Amperometric Detection INTRODUCTION The accurate measurement of the amount of sugar in final molasses
More informationSialic Acid Determination in Glycoproteins: Comparison of Two Liquid Chromatography Methods. Experimental. Introduction. Method 1.
Sialic Acid Determination in Glycoproteins: Comparison of Two Liquid Chromatography Methods Deanne Hurum and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Introduction Sialic acids are critical
More informationDetermination of Plant-Derived Neutral Oligo- and Polysaccharides Using the CarboPac PA200
Application Update 15 Determination of Plant-Derived Neutral Oligo- and Polysaccharides Using the CarboPac PA2 INTRODUCTION High-performance anion-exchange chromatography with pulsed amperometric detection
More informationDetermination of Clinically Relevant Compounds using HPLC and Electrochemical Detection with a Boron-Doped Diamond Electrode
Deteration of Clinically Relevant Compounds using HPLC and Electrochemical Detection with a Boron-Doped Diamond Electrode Bruce Bailey, Marc Plante, David Thomas, Qi Zhang and Ian Acworth Thermo Fisher
More informationDetermination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography
Application Note 65 Determination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography INTRODUCTION Preservatives are commonly added to many food products, such as soda, fruit juice,
More informationRapid determination of phosphate and citrate in carbonated soft drinks using ion chromatography
APPLICATION NOTE 7250 Rapid determination of phosphate and citrate in carbonated soft drinks using ion chromatography Authors Jingli Hu and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Keywords
More informationA Rapid UHPLC Method for the Analysis of Biogenic Amines and Metabolites in Microdialysis Samples
A Rapid UHPLC Method for the Analysis of Biogenic Aes and Metabolites in Microdialysis Samples Bruce Bailey and Ian Acworth; Thermo Fisher Scientific, Chelmsford, MA Overview Purpose: To develop an Ultra
More informationRapid and Direct Analysis of Free Phytosterols by Reversed Phase HPLC with Electrochemical Detection
Rapid and Direct Analysis of Free Phytosterols by Reversed Phase HPLC with Electrochemical Detection Bruce Bailey, Marc Plante, David Thomas, Qi Zhang and Ian Acworth Thermo Fisher Scientific, Chelmsford,
More informationImproved Extraction and Analysis of Hexavalent Chromium from Soil and Water
Improved Extraction and Analysis of Hexavalent Chromium from Soil and Water Richard F. Jack, 1 Jinshui Che, 2 Lipika Basumallick, 1 and Jeffrey Rohrer 1 1 Thermo Fisher Scientific, Sunnyvale, CA, USA;
More informationNitrogen/Protein Determination in Starch by Flash Combustion using Large Sample Weight as an Alternative to the Kjeldahl Method
Nitrogen/Protein Determination in Starch by Flash Combustion using Large Sample Weight as an Alternative to the Kjeldahl Method Liliana Krotz and Guido Giazzi Thermo Fisher Scientific, Milan, Italy Overview
More informationcolumns CarboPac PA20 Column
columns CarboPac PA Column Anion-exchange columns for the analysis of mono- and disaccharides in glycoprotein therapeutics, foods, and beverages: Simple, direct approach using pulsed electrochemical detection
More informationThe Investigation of Factors Contributing to Immunosuppressant Drugs Response Variability in LC-MS/MS Analysis
The Investigation of Factors Contributing to Immunosuppressant Drugs Variability in LC-MS/MS Analysis Joseph Herman, Dayana Argoti, and Sarah Fair Thermo Fisher Scientific, Franklin, MA, USA Overview Purpose:
More informationDetermination of Ethyl Sulfate Impurity in Indinavir Sulfate Drug Using Ion Chromatography
Application Note 8 Determination of Ethyl Sulfate Impurity in INTRODUCTION Indinavir sulfate is a specific and potent inhibitor of HIV- protease and is widely used in the treatment of AIDS. Indinavir sulfate
More informationGlycoprotein monosaccharide analysis using HPAE-PAD with manually prepared eluent
TECHNICAL NOTE 75 Glycoprotein monosaccharide analysis using HPAE-PAD with manually prepared eluent Authors Sachin Patil and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Keywords Glycoprotein,
More informationGC-MS/MS Analysis of Benzodiazepines Using Analyte Protectants
GC-MS/MS Analysis of Benzodiazepines Using Analyte Protectants Jeremy Matthews, 1 Alex Chen, 2 and Flavio Bedini 1 1 Thermo Fisher Scientific, Singapore; 2 Alpha Analytical Pte Ltd, Singapore Overview
More informationDetermination of Lactose in Lactose-Free Milk Products by High-Performance. anion-exchange chromatography with. Pulsed Amperometric Detection
Determination of Lactose in Lactose-Free Milk Products by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection Application Note 48 Introduction Lactose is the major disaccharide
More informationEnhanced LC-MS Sensitivity of Vitamin D Assay by Selection of Appropriate Mobile Phase
Enhanced LC-MS Sensitivity of Vitamin D Assay by Selection of Appropriate Mobile Phase Subhra Bhattacharya and Stephen C. Roemer Thermo Fisher Scientific Global Chemicals, Fair Lawn, NJ, USA AbstrAct Liquid
More informationHigh-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring Buprenorphine and Norbuprenorphine in Urine
High-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring and in Urine Xiaolei Xie, Joe DiBussolo, Marta Kozak; Thermo Fisher Scientific, San Jose, CA Application Note 627 Key Words, norbuprenorphine,
More informationHPAE-PAD Determination of Infant Formula Sialic Acid
HPAE-PAD Determination of Infant Formula Sialic Acid Deanna Hurum and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Application Note 53 Introduction Dietary sialic acids are important for infant
More informationComparison of Full Scan MS2 and MS3 Linear Ion Trap Approaches for Quantitation of Vitamin D
Comparison of Full Scan MS2 and MS3 Linear Ion Trap Approaches for Quantitation of Vitamin D Julie A. Horner 1, Marta Kozak 1, Subodh Nimkar 1, and August A. Specht 1 1 Thermo Fisher Scientific, San Jose,
More informationHigh-Throughput Quantitative LC-MS/MS Analysis of 6 Opiates and 14 Benzodiazepines in Urine
High-Throughput Quantitative LC-MS/MS Analysis of and 14 Benzodiazepines in Urine Bill Yu, Kristine Van Natta, Marta Kozak, Thermo Fisher Scientific, San Jose, CA Application Note 588 Key Words Opiates,
More informationDayana Argoti, Kerry Hassell, Sarah J. Fair, and Joseph Herman Thermo Fisher Scientific, Franklin, MA, USA
The Utilization of Novel Platform in a LC-MS/MS Workflow for the Analysis of Vitamin D, Testosterone, Immunosuppressants, Chemotherapeutics and Cortisol Dayana Argoti, Kerry Hassell, Sarah J. Fair, and
More informationFT-Raman Surface Mapping of Remineralized Artificial Dental Caries
FT-Raman Surface Mapping of Remineralized Artificial Dental Caries I. Stangel, 1 R. Rubinovitz, 2 G.D. Arndt, 3 D. Byerly, 3 C. Theriot, 3 J.E. Kerr, 4 1 BioMat Sciences, Bethesda, MD; 2 Thermo Fisher
More informationMeasuring Phytosterols in Health Supplements by LC/MS. Marcus Miller and William Schnute Thermo Fisher Scientific, San Jose, CA, USA
Measuring Phytosterols in Health Supplements by LC/MS Marcus Miller and William Schnute Thermo Fisher Scientific, San Jose, CA, USA Overview Purpose: Develop a method for the extraction of phytosterols
More informationDetermination of Inorganic Ions and Organic Acids in Non-Alcoholic Carbonated Beverages
Application Note Determination of Inorganic Ions and Organic Acids in Non-Alcoholic Carbonated Beverages INTRODUCTION The determination of inorganic anions and cations and organic acids in non-alcoholic
More informationQuantitative Analysis of THC and Main Metabolites in Whole Blood Using Tandem Mass Spectrometry and Automated Online Sample Preparation
Quantitative Analysis of THC and Main Metabolites in Whole Blood Using Tandem Mass Spectrometry and Automated Online Sample Preparation Valérie Thibert, Bénédicte Duretz Thermo Fisher Scientific, Courtaboeuf,
More informationDetermination of Sucralose in Reduced- Carbohydrate Colas using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection
Application Update 151 Determination of Sucralose in Reduced- Carbohydrate Colas using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection INTRODUCTION Sucralose (trichlorogalactosucrose
More informationThermo Scientific Dionex CarboPac PA100 Column
CHROMTOGRPHY Thermo Scientific Dionex CarboPac P Column Product Specifications HPLC Columns for Oligosaccharide Mapping and Purification Predictable, high resolution separations of oligosaccharides released
More informationHPAE-PAD determination of carbohydrates in honey to evaluate samples for quality and adulteration
APPLICATION NOTE HPAE-PAD determination of carbohydrates in honey to evaluate samples for quality and adulteration No. 8 Manali Aggrawal and Jeffrey Rohrer, Sunnyvale, CA, USA Key words Dionex CarboPac
More informationMS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine
Application Note: 346 MS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine Gargi Choudhary and Diane Cho, Thermo Fisher Scientific, San Jose, CA Wayne Skinner and
More informationImpurity Profiling of Carbamazepine by HPLC/UV
Application Note: 52049 Impurity Profiling of Carbamazepine by HPLC/UV Terry Zhang, Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela Hypersil GOLD Carbamazepine Drug Analysis
More informationHC-75 Calcium Form. 305 x 7.8 mm HC-75 Calcium Form (P/N 79436)
Page 1 of 5 U S A Example Applications HPLC Application Index Ordering Information Contact HPLC Support Polymeric cross-linked soft-gel columns for cation, ligand exchange separation of carbohydrates:
More informationEvaluation of an LC-MS/MS Research Method for the Analysis of 33 Benzodiazepines and their Metabolites
Evaluation of an LC-MS/MS Research Method for the Analysis of 33 Benzodiazepines and their Metabolites Valérie Thibert 1, Norbert Dirsch 2, Johannes Engl 2, Martin Knirsch 2 1 Thermo Fisher Scientific,
More informationAnalysis - Carbohydrate analysis
employ a technique called ligand exchange chromatography for the separation of monosaccharides, disaccharides and oligosaccharides up to 15 glucose units long. Ligand exchange resins are highly sulfonated
More informationHigh-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Carbohydrates Analysis Application Notebook
High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Carbohydrates Analysis Application Notebook Carbohydrates Analysis by HPAE-PAD High-Performance Anion-Exchange
More informationDirect Determination of Native N-linked Glycans by UHPLC with Charged Aerosol Detection
Direct Determination of Native N-linked Glycans by UHPLC with Charged Aerosol Detection David Thomas, Ian Acworth, Bruce Bailey, Marc Plante, and Qi Zhang Thermo Fisher Scientific, Chelmsford, MA Overview
More informationBioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS
Bioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS Jenny Chen, Hongxia Wang, Zhiqi Hao, Patrick Bennett, and Greg Kilby Thermo Fisher
More informationCarboPac PA-100 Column for Oligosaccharide Analysis
columns CarboPac PA- Column for Oligosaccharide Analysis HPLC Columns for Oligosaccharide Mapping and Purification Predictable, high resolution separations of oligosaccharides released from glycoproteins
More informationAnalysis of Emulsifiers in Foods by High Pressure Liquid Chromatography and Corona Charged Aerosol Detection
Analysis of Emulsifiers in Foods by High Pressure Liquid Chromatography and Corona Charged Aerosol Detection Marc Plante, Bruce Bailey, Ian N. Acworth Thermo Fisher Scientific, Chelmsford, MA Overview
More informationDetermination of Polydextrose in Foods by AOAC Method
Application Note 147 Determination of Polydextrose in Foods by AOAC Method 2000.11 INTRODUCTION Polydextrose is a synthetic polymer of glucose having mainly α1,6 linkages and an average degree of polymerization
More informationApplication Note. Authors: C. Ledesma, M. Gibert, J.R. Gibert Ingenieria Analitica S.L. Extracts from various food products
High-Performance Anion-Exchange Chromatography coupled with Pulsed Electrochemical Detection as a powerful tool to evaluate lactose content in lactose-free labeled products Application Note Authors: C.
More informationDetermination of Sucralose Using HPAE-PAD
Application Note 59 Determination of Sucralose Using HPAE-PAD INTRODUCTION Sucralose is used in the manufacture of nonnutritive sweetened food and beverage products. Sucralose, like most carbohydrates,
More informationIC-MS Environmental Applications - Water Testing. Application Notebook
IC-MS Environmental Applications - Water Testing Application Notebook Table of Contents Environmental Sample Using IC-MS 3 Environmental Applications: Water Testing Acrylamide in Water 4 Trace Urea in
More informationThermo Fisher Scientific, Sunnyvale, CA, USA; 2 Thermo Fisher Scientific, San Jose, CA, USA
An Ultra High Resolution Glycan Column for Isomeric Separation and the Structural Identification of Labeled N-Glycans from Proteins Including Antibodies Udayanath Aich, 1 Julian Saba, 2 Rosa Viner, 2 Shanhua
More informationOligosaccharide Analysis by High-Performance Anion- Exchange Chromatography with Pulsed Amperometric Detection
Oligosaccharide Analysis by High-Performance Anion- Exchange Chromatography with Pulsed Amperometric Detection Jeff Rohrer, Ph.D. Director, Applications Development, Dionex Products 1 The world leader
More informationSugars in Honey Using HPAE-PAD: What Is the Best Column?
IC COLUMNS FOR ANALYZING SUGARS IN HONEY Sugars in Honey Using HPAE-PAD: What Is the Best Column? Katrin Hostettler, 1 Robert Brogioli, 1 Silvio Arpagaus, 1 Beate Müller-Werner, 2 Detlef Jensen 2 1 Department
More informationIon Chromatography: A Versatile Technique for the Analysis of Beer
Application Note 6 Ion Chromatography: A Versatile Technique for the Analysis of Beer INTRODUCTION Ion chromatography is an efficient technique for the analysis and quantification of ions in solution.
More informationSolving One of Chromatography s Biggest Dilemmas Proper Sealing of Chromatography Autosampler Vials
Solving One of Chromatography s Biggest Dilemmas Proper Sealing of Chromatography Autosampler Vials D. Edwards 2, D. Lennartz 1, L.Shick 2 1 Thermo Fisher Scientific, Langerwehe, Germany 2 Thermo Fisher
More informationIdentification and Quantification at ppb Levels of Common Cations and Amines by IC-MS
Application Note 269 Identification and Quantification at ppb Levels of Common Cations and Amines by IC-MS INTRODUCTION Ion chromatography (IC) has been extensively used as the preferred separation technique
More informationMono- and Disaccharides
Application Note Food/Beverage/Life Sciences The finest LC-EC Applications for Food & Beverage analysis Mono- and Phenols Bisphenol A Catechins Flavonoids Phenols Antioxidants Resveratrol Epicatechin Quercetin
More informationLC-MS/MS Method for the Determination of 21 Opiates and Opiate Derivatives in Urine
LC-MS/MS Method for the Determination of 21 Opiates and Opiate Derivatives in Urine J. Jones, S. Westwood, T. Liddicoat, L. Pereira, T. Edge Thermo Fisher Scientific, Manor Park, Runcorn, UK Overview Purpose:
More informationAnalysis of Phospholipids in Natural Samples by Normal Phase HPLC and Corona Charged Aerosol Detection
Analysis of Phospholipids in Natural Samples by Normal Phase HPLC and Corona Charged Aerosol Detection Marc Plante, Bruce Bailey, Ian N. Acworth Thermo Fisher Scientific, Chelmsford, MA, USA Overview Purpose:
More informationDirect Determination of Sialic Acids in Glycoprotein Hydrolyzates by HPAE-PAD
Direct Determination of Sialic Acids in Glycoprotein Hydrolyzates by HPAE-PAD Thermo Fisher Scientific, Inc. Application Update 80 INTRODUCTION Sialic acids are critical in determining glycoprotein bioavailability,
More informationDiscrete Industrial Analyzer System Reagents for Food and Other Sample Materials
Discrete Industrial Analyzer System Reagents for Food and Other Sample Materials Analyte Code Product name Max. nbr. of tests/kit (1 Method Sample matrix Interference filter nm (2 Test ranges Unit Acetaldehyde
More informationIntegrated Targeted Quantitation Method for Insulin and its Therapeutic Analogs
Integrated Targeted Quantitation Method for Insulin and its Therapeutic Analogs Eric Niederkofler, 1 Dobrin Nedelkov, 1 Urban Kiernan, 1 David Phillips, 1 Kemmons Tubbs, 1 Scott Peterman, 2 Bryan Krastins,
More informationSugar analysis according to the EU Regulation using amperometric detection and Metrosep Carb 2-150/4.0
Sugar analysis according to the EU Regulation using amperometric detection and Metrosep Carb 2-150/4.0 Branch Food and beverages Bottle head 6.1602.200 Adsorber tube for Dosing Unit 6.1619.000 Keywords
More informationAnalysis of Food Sugars in Various Matrices Using UPLC with Refractive Index (RI) Detection
Analysis of Food Sugars in Various Matrices Using UPLC with Refractive Index (RI) Detection Mark E. Benvenuti Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Using UPLC with RI detection enables
More informationDynamic Analysis of HIV-Human Protein-Protein Interactions During Infection
Dynamic Analysis of HIV-Human Protein-Protein Interactions During Infection Jeffrey Johnson, 1 Shannon Eliuk, 2 Amnon Golan, 1 Tasha Johnson, 1 Vlad Zabrouskov, 2 Nevan Krogan 1 1 UCSF, San Francisco,
More informationANTEC LEYDEN. Analysis of Carbohydrates in Food and Beverages using Anion-Exchange Chromatography and Pulsed Amperometric. Introduction.
X Axis Title 220_002 #03 ANTEC LEYDEN System solutions from Antec Leyden... naturally! Analysis of Carbohydrates in Food and Beverages using Anion-Exchange Chromatography and Pulsed Amperometric Detection
More informationRapid Determination of Phosphate and Citrate in Carbonated Soft Drinks Using a Reagent-Free Ion Chromatography System
Application Note 69 Rapid Determination of Phosphate and Citrate in Carbonated Soft Drinks Using a Reagent-Free Ion Chromatography System INTRODUCTION Soft drinks are complex mixtures containing a variety
More informationConfident, lower-pressure analysis of carbohydrates, alcohols, and organic acids. Agilent Hi-Plex Ligand-Exchange HPLC Columns
Confident, lower-pressure analysis of carbohydrates, alcohols, and organic acids Agilent Hi-Plex Ligand-Exchange HPLC Columns Agilent Hi-Plex Ligand-Exchange HPLC columns Satisfy your growing demands for
More informationDetermination of Clinically Relevant Compounds using HPLC and Electrochemical Detection with Boron-Doped Diamond Electrode
Deteration of Clinically Relevant Compounds using HPLC and Electrochemical Detection with Boron-Doped Diamond Electrode Bruce Bailey, Marc Plante, David Thomas, Qi Zhang and Ian Acworth Thermo Fisher Scientific,
More informationDetermination of Olive Oil Adulteration by Principal Component Analysis with HPLC Charged Aerosol Detector Data
Determination of Olive Oil Adulteration by Principal Component Analysis with HPLC Charged Aerosol Detector Data Marc Plante, Bruce Bailey and Ian N. Acworth Thermo Fisher Scientific, Chelmsford, MA, USA
More informationEnhancing Sequence Coverage in Proteomics Studies by Using a Combination of Proteolytic Enzymes
Enhancing Sequence Coverage in Proteomics Studies by Using a Combination of Proteolytic Enzymes Dominic Baeumlisberger 2, Christopher Kurz 3, Tabiwang N. Arrey, Marion Rohmer 2, Carola Schiller 3, Thomas
More informationApplication Note. Sensitive analysis of the lactose content of lactose-free labelled products using HPAEC-PAD. Summary.
Application Note Sensitive analysis of the lactose content of lactose-free labelled products using HPAEC-PAD Category Food analysis Matrix Extracts from various food products Method HPAEC-PAD Keywords
More informationThe Determination of Carbohydrates, Alcohols, and Glycols in Fermentation Broths
The Determination of Carbohydrates, Alcohols, and Glycols in Fermentation Broths Valoran Hanko and Jeffrey S. Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Application Note 122 Introduction Fermentation
More informationIncreasing Extraction Efficiency of Wet Samples Using a Novel New Polymer During Accelerated Solvent Extraction
Increasing Extraction Efficiency of Wet Samples Using a Novel New Polymer During Accelerated Solvent Extraction SM Rahmat Ullah, Kannan Srinivasan, Chris Pohl, Pranathi Perati and Alexander Zhang Thermo
More informationCarbohydrates in Food
Application Note Food & Beverage The finest LC-EC Applications for Food & Beverage analysis Carbohydrates Phenols Bisphenol A Catechins Flavonoids Phenols Antioxidants Resveratrol Epicatechin Quercetin
More informationDetermination of Polymerized Triglycerides by High Pressure Liquid Chromatography and Corona Veo Charged Aerosol Detector
Determination of Polymerized Triglycerides by High Pressure Liquid Chromatography and Corona Veo Charged Aerosol Detector Qi Zhang, Bruce Bailey, Dave Thomas, Marc Plante, and Ian Acworth Thermo Fisher
More informationAPPLICATIONS TN Determination of Sugars in Animal Feed using HPLC-ELSD
Determination of Sugars in Animal Feed using HPLC-ELSD Laura Snow, Zeshan Aqeel, Allen Misa and Scott Krepich Phenomenex, Inc., Madrid Ave., Torrance, CA 9050 USA Laura Snow Scientist- Phenologix Outside
More informationHypersil BDS and Hypersil Classical HPLC Columns
LC Columns and Accessories Hypersil BDS and Hypersil Classical HPLC Columns Exceptionally reliable and reproducible general purpose columns Exceptionally reliable robust columns that guarantee you have
More informationThermo Scientific LipidSearch Software for Lipidomics Workflows. Automated Identification and Relative. Quantitation of Lipids by LC/MS
Thermo Scientific LipidSearch Software for Lipidomics Workflows Automated Identification and Relative of Lipids by LC/MS The promise of lipidomics Lipidomics is a new field of study crucial for understanding
More informationDetermination of Phenolic Compounds in Apple Orchard Soil
Determination of Phenolic Compounds in Apple Orchard Soil Che Jinshui, Xu Qun, Liang Lina, and Jeffrey Rohrer Thermo Fisher Scientific, Shanghai, People s Republic of China; Thermo Fisher Scientific, Sunnyvale,
More informationCatalogue. Resins and Columns For High Performance Liquid Chromatography
Catalogue Resins and Columns For High Performance Liquid Chromatography Updated August 11, 2008 Contents Benson Polymeric provides premium polymeric column packing materials and prepacked columns for use
More informationSPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine
SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 709 Key Words SPE, SOLA
More information[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector
Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Mark Benvenuti, Gareth Cleland, and Jennifer Burgess Waters Corporation, Milford, MA, USA
More informationSeparation of 2AA-Labeled N-Linked Glycans from Glycoproteins on a High Resolution Mixed-Mode Column
Separation of 2AA-Labeled N-Linked Glycans from Glycoproteins on a High Resolution Mixed-Mode Column Udayanath Aich, Julian Saba, Xiaodong Liu, Jeff Rohrer, Jim Thayer and Chris Pohl, Thermo Fisher Scientific,
More informationDetermination of Plant-Derived Neutral Oligo- and Polysaccharides
Determination of Plant-Derived Neutral Oligo- and Polysaccharides Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Application Note 67 Introduction Since it was first reported that high-performance
More informationDetermination of Organic Acids in Kombucha Using a High-Pressure Ion Chromatography System
APPLICATION NOTE Determination of Organic Acids in Kombucha Using a High-Pressure Ion Chromatography System No. 1157 Beibei Huang, Jingli Hu, and Jeffrey Rohrer Keywords Dionex IonPac AS11-HC-4µm column,
More informationCharacterization of Used Cooking Oils by High Performance Liquid Chromatography and Corona Charged Aerosol Detection
Characterization of Used Cooking Oils by High Performance Liquid Chromatography and Corona Charged Aerosol Detection Marc Plante, Bruce Bailey, and Ian N. Acworth Thermo Fisher Scientific, Chelmsford,
More informationUse of a Tandem Mass Spectrometry Research Method for the Analysis of Amino Acids and Acylcarnitines in Dried Blood Spots
Use of a Tandem Mass Spectrometry Research Method for the Analysis of Amino Acids and Acylcarnitines in Dried Blood Spots Xiaolei Xie and Marta Kozak Thermo Fisher Scientific, San Jose, CA, USA Application
More informationApplication Note 178. Brian DeBorba and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA. 10 Typically, this requires the use of sample
Improved Determination of Trace Concentrations of Perchlorate in Drinking Water Using Preconcentration with Two-Dimensional Ion Chromatography and Suppressed Conductivity Detection Brian DeBorba and Jeff
More informationPIF: Precursor Ion Fingerprinting Searching for a Structurally Diagnostic Fragment Using Combined Targeted and Data Dependent MS n
Application Note: 441 PIF: Precursor Ion Fingerprinting Searching for a Structurally Diagnostic Fragment Using Combined Targeted and Data Dependent MS n Julie A. Horner, Rohan A. Thakur, Thermo Fisher
More informationAbstract for High Fructose Syrup Production. Ensymm abstract for enzymatic high fructose syrup production.
Abstract for High Fructose Syrup Production Ensymm abstract for enzymatic high fructose syrup production. 1 INTRODUCTION INVERT The food SUGAR and ABSTRACT beverage industry The depends food and heavily
More informationSensitive Analysis of Commonly Used Artificial and Natural Sweeteners Including Stevia and Their Impurities and Degradation Products
Sensitive Analysis of Commonly Used Artificial and Natural Sweeteners Including Stevia and Their Impurities and Degradation Products Ian Acworth, Chris Crafts, and Bruce Bailey, Thermo Fisher Scientific,
More informationImproving Food Process Control Using the
Improving Food Process Control Using the UPLC Amino Acid Analysis Solution Hillary B. Hewitson, Thomas E. Wheat, Diane M. Diehl INTRODUCTION Food products have different desirability and value based in
More informationA New HILIC/RP Mixed-Mode Column and Its Applications in Surfactant Analysis
A New HILIC/RP Mixed-Mode Column and Its Applications in Surfactant Analysis X. Liu, C. Pohl, Dionex Corporation, Sunnyvale, CA, USA ABSTRACT Although reversed-phase (RP) silica columns (e.g., C18 and
More informationDetermination of Trace Sodium in Cranberry Powder
Determination of Trace Sodium in Cranberry Powder Brian De Borba and Jeffrey Rohrer; Thermo Fisher Scientific Inc., Sunnyvale, CA, USA Application Note 00 Key Words Dietary Supplements, Ion Chromatography,
More informationLC-MS/MS Method for the Determination of Tenofovir from Plasma
LC-MS/MS Method for the Determination of Tenofovir from Plasma Kimberly Phipps, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 687 Key Words SPE, SOLA CX, Hypersil GOLD, tenofovir Abstract
More informationGlycanPac AXR-1 Columns
CHRMATGRAPHY GlycanPac AXR- Columns For High Resolution Glycan Analysis Product Specifications The Thermo Scientific GlycanPac AXR- columns are highperformance, silica-based HPLC columns for simultaneous
More informationRapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column
APPLICATION NOTE 21673 Rapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column Authors Aaron Lamb and Brian King, Thermo Fisher Scientific, Runcorn, UK
More informationBio-Rad Laboratories HEMOGLOBIN Testing Bio-Rad A1c. VARIANT II TURBO Link. Fully-Automated HbA 1c Testing
Bio-Rad Laboratories HEMOGLOBIN Testing Bio-Rad A1c VARIANT II TURBO Link Fully-Automated HbA 1c Testing Bio-Rad Laboratories HEMOGLOBIN Testing VARIANT II TURBO Link Efficient streamlined process Powerful
More informationBio-Rad Laboratories HEMOGLOBIN Testing Bio-Rad A1c. VARIANT II TURBO Link System. Fully-Automated HbA 1c Testing
Bio-Rad Laboratories HEMOGLOBIN Testing Bio-Rad A1c VARIANT II TURBO Link System Fully-Automated HbA 1c Testing Bio-Rad Laboratories HEMOGLOBIN Testing VARIANT II TURBO Link System Efficient streamlined
More information