HC-75 Calcium Form. 305 x 7.8 mm HC-75 Calcium Form (P/N 79436)

Transcription

1 Page 1 of 5 U S A Example Applications HPLC Application Index Ordering Information Contact HPLC Support Polymeric cross-linked soft-gel columns for cation, ligand exchange separation of carbohydrates: Separate Mono and Disaccharides Separate oligomers from DP1 to DP8 Compatible with RI detectors. Product Features HC-40 One particle size: 9 µm One column internal diameter: 7.8 mm. One column material: 316 stainless steel HC-75 One particle size: 9 µm Three column internal diameters: 4.1, 7.8 and 10.0 mm One column material: 316 stainless steel Three forms: calcium, lead and hydrogen Applications HC-40 Calcium Form HC-75 Calcium Form Corn Syrup High Conversion Corn Syrup Beer HC-75 Lead Form Sugar Alcohol Standards HC-75 Hydrogen Form Sugars and Organic Acids Corn Syrup Chewing Gum Sweeteners Milk Product Sugars Plant Cell Wall Hydrolysates Glycols and Polyols High Fructose Corn Syrup Standards Apple Juice Pineapple Juice Hamilton HC-40 and HC-75 Column Benefits High Flow Rates Up to 1.2 ml/min on a 305 x 7.8 mm column with only 400 psi of back pressure on an HC-75 column. HC-40 columns can be used with flow rates up to 0.5 to 0.6 ml/min on a 305 x 7.8 mm column with only 400 psi of back pressure. Aqueous Mobile Phases Both columns use water as a mobile phase which simplifies eluent preparation and minimizes cost. Application #126 and #91 illustrate the difference in separation that HC-40 and HC-75 provide for an identical sample under the same separation conditions. HC-75 only separates the first four components, while the porous HC-40 column provides a more complete size exclusion type separation. Corn Syrup Conditions: Deionized Water. Isocratic. 80 C. Corn Syrup 305 x 7.8 mm HC-40 (P/N 79432)

2 Page 2 of 5 7. Maltooctaose + Conditions: Water. Isocratic. 80 C. Injection: 12 µl Application #121 is a high conversion corn syrup sample on HC-40. With this type of sample most of the higher oligosaccharides have been converted to glucose or fructose to increase the corn syrup's commercial value. High Conversion Corn Syrup 7. Maltoheptaose 8. Maltooctaose + Conditions: Deionized Water. Isocratic 80 C Injection: 20 µl Application #130 and #131 are both beer separations on HC-40. During the fermentation process the majority of the simple sugars are fermented into ethanol. The small differences in unfermented carbohydrates help to give each beer its distinct flavor. Brand X Beer 1. Ethanol Conditions: Deionized Water. Isocratic. 90 C Brand Y Beer 1. Ethanol Conditions: Deionized Water Isocratic. 90 C

3 Page 3 of 5 Application #125 illustrates the analysis of some sweeteners commonly used in chewing gum. Sucrose, glucose, fructose, mannitol and sorbitol are well resolved on the HC-75 calcium form column. Chewing Gum 2. Glucose 3. Fructose 4. Mannitol 5. Sorbitol Flow: 1.2 ml/min. Injection: 20 µl The wide applicability of HC-75 calcium form columns is shown in Applications #122 and #123 with the separation of milk product sugars and plant cell wall hydrolysates. Milk Product Sugars 2. Lactose 3. Glucose 4. Fructose Flow: 0.4 ml/min. Plant Cell Wall Hydrolysates 2. Glucose 3. Xylose 4. Arabinose Flow: 0.5 ml/min.

4 Page 4 of 5 In the case of glycols and polyols, an incomplete separation can be improved with the addition of up to 40% acetonitrile. Application #124 illustrates how the separation can be improved by changing the temperature and acetonitrile concentration. Glycols and Polyols 1. Glycerol 2. Ethylene Glycol 3. Propylene Glycol 4. Inositol 5. Ribitol 6. Erythritol 7. Arabitol 8. Mannitol 9. Xylitol 10. Galactitol 11. Sorbitol Conditions: 60:40 Deionized Water: Acetonitrile. Isocratic. 78 C. Flow: 1.0 ml/min. Application #117 shows the different selectivity that is possible with a lead counter ion instead of calcium. Lead form HC-75 columns are best suited for the separation of sugar alcohols. Sugar Standards 305 x 7.8 mm HC-75 Lead Form (P/N 79438) 2.5 mg/ml 2. Glucose 2 5 mg/ml 3. Fructose 2.5 mg/ml 4. Inositol 2.5 mg/ml 5. Sorbitol 2 5 mg/ml Conditions: Deionized Water. Isocratic. 80 C. Injection: 200 µl EXAMPLE APPLICATION #91 Corn Syrup on HC-40 #117 Sugar Standards #121 High Conversion Corn Syrup #122 Milk Product Sugars #123 Plant Cell Wall Hydrolysates #124 Glycols and Polyols #125 Chewing Gum #126 Corn Syrup on HC-75 #130 Brand X Beer #131 Brand Y Beer ORDERING INFORMATION HC-40 Gel-type Cation Exchange Columns Particle Size I.D. x Length µm 7.8 X 305 mm HC-75 Gel-type Cation Exchange Columns Particle Size and Resin Form

5 Page 5 of 5 I.D. x Length 9 µm Calcium 9 µm Lead 9 µm Hydrogen 4.1 x 250 mm x 100 mm x 305 mm x 250 mm Guard Columns for HC-40 and HC-75 Columns For Steel Columns Hydrogen Form Cation Exchange Starter Kit (1 holder, 1 cartridge) Hydrogen Form Cation Exchange Replacement Cartridges (2/pk) Carbonate Form Anion Exchange Starter Kit (1 holder, 1 cartridge) Carbonate Form Anion Exchange Replacement Cartridges (2/pk) Guard Column Technical Information Column Care and Storage About Hamilton Contact Quick Order Service News & Promos Copyright Hamilton Company. All Rights Reserved. Terms of Use. Privacy Statement.