GC/MS BATCH NUMBER: J10105
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1 GC/MS BATCH NUMBER: J10105 ESSENTIAL OIL: JASMINE ABSOLUTE BOTANICAL NAME: JASMINUM SAMBAC ORIGIN: INDIA KEY CONSTITUENTS PRESENT IN THIS BATCH OF JASMINE ABSOLUTE OIL % (3E,6E)-α-FARNESENE 12.0 BENZYL ALCOHOL 11.3 BENZYL ACETATE 7.4 LINALOOL 6.7 (3Z)-HEXENYL BENZOATE 6.1 METHYL ANTHRANILATE 5.9 (9Z)-TRICOSENE 4.0 α-linolenic ACID 2.5 INDOLE 1.9 BENZENEACETONITRILE 1.8 METHYL Α-LINOLENATE 1.8 PALMITIC ACID 1.5 PHENYLETHYL ALCOHOL 1.4 SQUALENE 1.1 GERMACRENE D-4-OL 1.1 BENZYL α-linolenate 1.1 Comments from Robert Tisserand: Sophisticated, fresh-green floral odor quality. Constituents are in expected amounts nd St S. Twin Falls, ID * * planttherapy.com facebook.com/planttherapy * planttherapy.com/blog
2 Date : February 07, 2018 CERTIFICATE OF ANALYSIS - GC PROFILING SAMPLE IDENTIFICATION Internal code : 18B01-PTH14-1-CC Customer identification : Jasmine Absolute - India - J R Type : Essential oil Source : Jasminum sambac Customer : Plant Therapy ANALYSIS Method: PC-PA J19 - Analysis of the composition of an essential oil, or other volatile liquid, by FAST GC-FID (in French); identifications validated by GC-MS. Analyst : Alexis St-Gelais, M. Sc., chimiste Analysis date : February 07, 2018 Checked and approved by : Alexis St-Gelais, M. Sc., chimiste Note: This report may not be published, including online, without the written consent from Laboratoire PhytoChemia. This report is digitally signed, it is only considered valid if the digital signature is intact. Page 1/12
3 PHYSICOCHEMICAL DATA Physical aspect: Orange brownish liquid Refractive index: ± (20 C) CONCLUSION No adulterant, contaminant or diluent has been detected using this method. Please take note that since some compounds are fully retained on the DB-Wax column, a systematic deviation in the percentages between both columns is induced; prefer the percentage on the DB-5 column for reference. Page 2/12
4 ANALYSIS SUMMARY Identification DB-5 (%) DB-WAX (%) Classe Ethanol Aliphatic alcohol Acetic acid tr 0.01 Aliphatic acid Ethyl acetate Aliphatic ester Hexan-3-ol Aliphatic alcohol Hexan-2-ol Aliphatic alcohol (3E)-Hexenol tr 0.01 Aliphatic alcohol (3Z)-Hexenol Aliphatic alcohol (2E)-Hexenol Aliphatic alcohol Hexanol Aliphatic alcohol (3Z)-Hexenyl formate tr Aliphatic ester Prenyl acetate tr 0.01 Aliphatic ester Benzaldehyde Simple phenolic (3Z)-Hexenyl acetate Aliphatic ester Hexyl acetate Aliphatic ester (2E)-Hexenyl acetate Aliphatic ester Hexanoic acid Aliphatic acid Benzyl alcohol 9.57* Simple phenolic (Z)-β-Ocimene [9.57]* tr Monoterpene (E)-β-Ocimene Monoterpene cis-linalool oxide (fur.) Monoterpenic alcohol Benzyl formate Phenolic ester trans-linalool oxide (fur.) Monoterpenic alcohol Methyl benzoate Phenolic ester Linalool Monoterpenic alcohol Phenylethyl alcohol Simple phenolic Benzeneacetonitrile Simple phenolic Benzyl acetate * Phenolic ester Ethyl benzoate [6.36]* 0.14* Phenolic ester cis-linalool oxide (pyr.) [6.36]* 0.01 Monoterpenic alcohol Unknown Unknown trans-linalool oxide (pyr.) * Monoterpenic alcohol (3Z)-Hexenyl butyrate Aliphatic ester Methyl salicylate 0.28 [13.77]* Phenolic ester Benzoic acid 0.31 Simple phenolic (3Z)-Hexenyl 2-methylbutyrate Aliphatic ester (3Z)-Hexenyl isovalerate * Aliphatic ester Phenylethyl acetate Phenolic ester Unknown 0.33* 0.36 Unknown Geraniol [0.33]* 0.09 Monoterpenic alcohol (E)-Isogeraniol? Monoterpenic alcohol Ethyl salicylate Phenolic ester Phenylacetic acid? [0.15] Phenolic acid Unknown 0.21 Unknown 2,6-Dimethyl-1,7-octadiene-3,6-diol * Monoterpenic alcohol Indole 1.57* 1.87 Indole 1-Nitro-2-phenylethane [1.57]* 0.22* Simple phenolic (E)-Cinnamyl alcohol Phenylpropanoid Methyl anthranilate * Phenolic ester Page 3/12
5 Eugenol Phenylpropanoid 8-Hydroxylinalool isomer 0.29* 0.26 Monoterpenic alcohol Neryl acetate [0.29]* 0.11 Monoterpenic ester Butyl benzoate 0.04* 0.01 Phenolic ester α-copaene [0.04]* [0.01]* Sesquiterpene Methyl (E)-cinnamate Phenylpropanoid ester β-cubebene 0.05* 0.01 Sesquiterpene (3Z)-Hexenyl hexanoate? [0.05]* Aliphatic ester (3Z)-Hexenyl (3Z)-hexenoate 0.15* 0.10 Aliphatic ester β-elemene [0.15]* 0.09 Sesquiterpene (Z)-Jasmone Jasmonate Dimethyl anthranilate * Phenolic ester Ethyl anthranilate 0.02 Phenolic ester β-caryophyllene Sesquiterpene α-humulene 0.07* [0.14]* Sesquiterpene (E)-Cinnamyl acetate [0.07]* [0.08]* Phenylpropanoid ester (E)-β-Farnesene Sesquiterpene Oxindole? 0.03 Indole γ-muurolene Sesquiterpene Germacrene D Sesquiterpene Bicyclogermacrene 0.16 [7.36]* Sesquiterpene epi-cubebol Sesquiterpenic alcohol (3Z,6E)-α-Farnesene 0.26* 0.15 Sesquiterpene α-muurolene [0.26]* [7.36]* Sesquiterpene (3E,6E)-α-Farnesene 12.22* [13.77]* Sesquiterpene γ-cadinene [12.22]* 0.26 Sesquiterpene δ-cadinene Sesquiterpene 10-epi-Cubebol? 0.03 Sesquiterpenic alcohol α-cadinene Sesquiterpene Methyl N-formylanthranilate Phenolic ester Hexenyl benzoate isomer 0.05 [0.22]* Phenolic ester (E)-Nerolidol Sesquiterpenic alcohol (3Z)-Hexenyl benzoate * Phenolic ester Hexyl benzoate 1.61* 0.11 Phenolic ester Germacrene D-4-ol [1.61]* [2.81]* Sesquiterpenic alcohol (2E)-Hexenyl benzoate [1.61]* 0.41 Phenolic ester Spathulenol 0.10 [5.79]* Sesquiterpenic alcohol Methyl N-acetylanthranilate Phenolic ester Ledol Sesquiterpenic alcohol τ-cadinol 0.19* 0.12 Sesquiterpenic alcohol τ-muurolol [0.19]* 0.12 Sesquiterpenic alcohol α-muurolol Sesquiterpenic alcohol α-cadinol Sesquiterpenic alcohol Unknown 0.11 Unknown (3E,5E)-7-Hydroxyfarnesene 0.10 Sesquiterpenic alcohol Shyobunol Sesquiterpenic alcohol Unknown Unknown (2E,6E)-Farnesol Sesquiterpenic alcohol (E)-Coniferyl alcohol 0.15 Phenylpropanoid Oplopanone * Sesquiterpenic alcohol Unknown 0.37 [0.47]* Unknown Benzyl benzoate * Phenolic ester Page 4/12
6 Unknown 0.14 Unknown (2E,6E)-Farnesyl acetate Sesquiterpenic ester Phenylethyl benzoate Phenolic ester Methyl palmitate Aliphatic ester meta-camphorene 0.04 [6.78]* Diterpene Palmitic acid 1.20* 1.51 Aliphatic acid para-camphorene [1.20]* 0.01 Diterpene (E,E)-Geranyllinalool Diterpenic alcohol (E)-Cinnamyl benzoate 0.20 Phenylpropanoid ester Methyl linoleate Aliphatic ester Methyl α-linolenate Aliphatic ester Methyl stearate Aliphatic ester α-linolenic acid Aliphatic acid Ethyl α-linolenate? 0.10 [0.54]* Aliphatic ester Docosene isomer Alkene Unknown 0.16 Unknown (9Z)-Eicosenol? 0.13 Aliphatic alcohol (9Z)-Tricosene Alkene Methyl arachidate Aliphatic ester Tetracosene isomer 0.29 Alkene Unknown 0.39 Unknown Benzyl palmitate 0.22 Phenolic ester Benzyl oleate 0.14* Phenolic ester Benzyl linoleate [0.14]* Phenolic ester Benzyl α-linolenate 1.08 Phenolic ester Squalene Triterpene 2,3-Oxidosqualene 0.95 Triterpenic ether α-tocopherol? 0.06 Tocopherol Unknown 1.67 Unknown Unknown 4.01 Unknown (3Z)-Hexenyl propionate? 0.01 Aliphatic ester Total identified 79.15% 85.57% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied Page 5/12
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10 FULL ANALYSIS DATA Identification Column DB-5 Column DB-WAX R.T R.I % R.T R.I % Ethanol Acetic acid tr Ethyl acetate Hexan-3-ol Hexan-2-ol (3E)-Hexenol tr (3Z)-Hexenol (2E)-Hexenol Hexanol (3Z)-Hexenyl formate tr Prenyl acetate tr Benzaldehyde (3Z)-Hexenyl acetate Hexyl acetate (2E)-Hexenyl acetate Hexanoic acid Benzyl alcohol 4.69* (Z)-β-Ocimene 4.69* 1038 [9.57] tr (E)-β-Ocimene cis-linalool oxide (fur.) Benzyl formate trans-linalool oxide (fur.) Methyl benzoate Linalool Phenylethyl alcohol Benzeneacetonitrile Benzyl acetate * Ethyl benzoate 6.71* 1167 [6.36] 9.40* cis-linalool oxide (pyr.) 6.71* 1167 [6.36] Unknown [m/z 43, 69 (35), 41 (26), 83 (25), 57 (22) (1)] trans-linalool oxide (pyr.) * (3Z)-Hexenyl butyrate Methyl salicylate * 1716 [13.77] Benzoic acid (3Z)-Hexenyl 2- methylbutyrate (3Z)-Hexenyl * Page 9/12
11 isovalerate Phenylethyl acetate Unknown [m/z 91, 117 (80), 90 (42), 8.04* (29) (5)] Geraniol 8.04* 1255 [0.33] (E)-Isogeraniol? Ethyl salicylate Phenylacetic acid? [0.15] Unknown [m/z 91, 117 (98), 90 (65), 89 (29), 65 (29), 118 (26), 135 (23)] ,6-Dimethyl-1,7- octadiene-3,6-diol * Indole 8.58* Nitro-2- phenylethane 8.58* 1290 [1.57] 14.34* (E)-Cinnamyl alcohol Methyl anthranilate * Eugenol Hydroxylinalool 9.61* isomer Neryl acetate 9.61* 1363 [0.29] Butyl benzoate 9.68* α-copaene 9.68* 1368 [0.04] 7.25* 1446 [0.01] Methyl (E)- cinnamate β-cubebene 9.89* (3Z)-Hexenyl hexanoate? 9.89* 1383 [0.05] (3Z)-Hexenyl (3Z)- hexenoate 9.94* β-elemene 9.94* 1386 [0.15] (Z)-Jasmone Dimethyl * anthranilate Ethyl anthranilate β-caryophyllene α-humulene 10.73* * 1612 [0.14] (E)-Cinnamyl 10.73* 1444 [0.07] 14.78* 2086 [0.08] acetate (E)-β-Farnesene Oxindole? γ-muurolene Germacrene D Bicyclogermacrene * 1678 [7.36] epi-cubebol (3Z,6E)-α-Farnesene 11.40* α-muurolene 11.40* 1494 [0.26] 10.22* 1678 [7.36] (3E,6E)-α-Farnesene 11.62* * 1716 [13.77] Page 10/12
12 γ-cadinene 11.62* 1512 [12.22] δ-cadinene epi-Cubebol? α-cadinene Methyl N- formylanthranilate Hexenyl benzoate isomer * 2044 [0.22] (E)-Nerolidol (3Z)-Hexenyl benzoate * Hexyl benzoate 12.37* Germacrene D-4-ol 12.37* 1570 [1.61] 13.83* 1995 [2.81] (2E)-Hexenyl benzoate 12.37* 1570 [1.61] Spathulenol * 2064 [5.79] Methyl N acetylanthranilate Ledol τ-cadinol 13.17* τ-muurolol 13.17* 1634 [0.19] α-muurolol α-cadinol Unknown [m/z 99, 161 (100), 43 (92), 204 (74), 71 (73), 121 (65)...] (3E,5E)-7- Hydroxyfarnesene Shyobunol Unknown [m/z 99, 43 (47), 161 (42), 71 (39), 204 (31), 121 (28)...] (2E,6E)-Farnesol (E)-Coniferyl alcohol Oplopanone * Unknown [m/z 105, 77 (42), 69 (29), 161 (19), 83 (16)...] * 2467 [0.47] Benzyl benzoate * Unknown [m/z 43, 159 (79), 93 (49), 119 (48), 161 (40), 187 (36) ? (2)] (2E,6E)-Farnesyl acetate Phenylethyl benzoate Methyl palmitate meta-camphorene * 2166 [6.78] Page 11/12
13 Palmitic acid 16.98* para-camphorene 16.98* 1974 [1.20] (E,E)-Geranyllinalool (E)-Cinnamyl benzoate Methyl linoleate Methyl α-linolenate Methyl stearate α-linolenic acid Ethyl α-linolenate? * 2541 [0.54] Docosene isomer Unknown [m/z 190, 158 (100), 253 (68), 193 (58), 220 (51)] (9Z)-Eicosenol? (9Z)-Tricosene Methyl arachidate Tetracosene isomer Unknown [m/z 219, 218 (99), 217 (50), 108 (31), 220 (17), 216 (14)] Benzyl palmitate Benzyl oleate 23.86* Benzyl linoleate 23.86* 2743 [0.14] Benzyl α-linolenate Squalene ,3-Oxidosqualene α-tocopherol? Unknown [m/z 322, 245 (66), 122 (37), 204 (34), 321 (30), 323 (26)...] Unknown [m/z 245, 246 (20), 243 (14), 217 (12), 218 (9), 91 (7), 244 (6)] (3Z)-Hexenyl propionate? Total identified 79.15% 85.57% Total reported 86.84% 86.67% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total : Peaks apexes were resolved, but peaks overlapped and were summed for analysis tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied R.T.: Retention time (minutes) R.I.: Retention index Page 12/12
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