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1 SUPPLEMENTARY INFORMATION doi:1.18/nture129 ontrol-dna -DNA CD49 Blood Lung e.98 +/ / / /-.6 Bsophils (x1 )/ml 4 Bsophils ( x1 ) d f ontrol-dna ontrol-dna -DNA -DNA Bone mrrow. +/ /-. IgE Bsophils (x1 ) 4 15 ontrol-dna -DNA Supplementry Figure 1. promotes peripherl sophili nd Th2 ytokine responses. BALB/ mie were injeted (i.v.) with ontrol DNA plsmid (ontrol-dna) or enoding plsmid (-DNA), nd () lood, () lungs nd (e) one mrrow were hrvested 2-21 dys post-plsmid injetion, proessed nd stined for sophils. Flow ytometry plots re gted on live, NBNT ells. Bsophils were identified s NBNT, CD49+, IgE+, + nd -Kit-. (,d,f), Totl sophil numers for eh omprtment were determined. Results re representtive of two seprte experiments of 5 mie per group. Sttistil nlysis ws performed using two-tiled students t test (, p<.5), (, p<.1), (, p<.1). 1
2 RESEARCH SUPPLEMENTARY INFORMATION d 5 4 IL-5 IL-1 15 IgE Bsophils (1 ) 175 (pg/ml ) 2 (pg/ml ) 75 (pg/ml ) 15 e Nive INF Nive INF + sos g + sos + sos Nive Infeted Time (d) sos RELM β f h 2 Worm urden Nive Infeted Infeted + sos 1 + sos Supplementry Figure 2. Bsophil-restrited R expression is suffiient to reover Th2 ell-dependent immunity to helminth infetion. (), or Tslpr-/- mie were infeted with Trihuris muris, nd spleni sophil popultions were quntified on dy 21 post-infetion. Results re ompiled from seprte experiments. (-h), or Tslpr-/- were infeted with T. muris. One group of infeted Tslpr-/- mie reeived 1-1 x 1 R-suffiient sophils (+ sos). Mesenteri LN ells were isolted nd stimulted with T. muris ntigen olleted from dult worms tht were ultured for 4 hours in medi. Antigen-speifi prodution of () IL-5 nd () IL-1 ws determined y ELISA. (d), Serum IgE levels, (e, f) golet ell hyperplsi nd intestinl muin prodution (PAS stining), (g) luminl seretion of RELMβ nd (h) worm urdens were determined on dy 21 post-infetion. (), Results re ompiled from seprte experiments. (-h), Results re representtive of two seprte experiments. ( mie n=1, Tslpr-/- n=1, Tslpr-/- mie tht reeived 1-1 x 1 sophils n=6.) Sle rs, 1 μm. Sttistil nlysis ws performed using two-tiled students t test (, p<.5), (,p<.1). (IgE levels etween Tslpr-/- nd Tslpr-/- + sos, p=.8). 2
3 SUPPLEMENTARY INFORMATION RESEARCH SSC mst ells Medi. sophils 89.7 d.84 mst ells CD e 6. f.88 mst ells CD, CD8,CD4, CD19 -Kit g 1 Bsophils 1 h Mst Cells ell numer 1 1 ell numer Medi Medi Supplementry Figure. seletively expnds sophil ut not mst ell popultions from one mrrowresident ells. Bone mrrow ws tken from mie nd ultured in the presene of () medi, () or (e) for 5 dys. (, d, f), Bsophils were identified s NBNT, CD49+, +, -Kit-. Mst ells were identified s NBNT, +, -Kit+. (g), Totl numers of sophils nd (h) mst ells were quntified on dy 5 post-ulture. (), Results re representtive of three seprte experiments. (g, h), Cell numers were ompiled from three seprte experiments.
4 RESEARCH SUPPLEMENTARY INFORMATION -Kit BP.76 FMO BP / Tslpr BP SSC IL-7Rα NBNT one mrrow BPs Kit R sort CD4 Input ell 5 dy ulture Medi d CD4 CD e 1 post ulture f Medi live ells Medi Supplementry Figure 4. ts diretly on one mrrow-resident preursor ells. (), Bsophil preursors from the one mrrow of or Tslpr-/- nimls were identified using BD FACS Ari II flow ytometer s NBNT, CD4+, +, -Kit-. Bsophil preursor expression levels of IL-7Rα (shded) were ompred to fluoresene minus one (FMO) ontrols. Bsophil preursor expression levels of R (shded) were ompred to sophil preursors in the one mrrow of Tslpr-/- mie. (), Bsophil preursors (NBNT, CD4+, +, -Kit-) were sorted using BD FACS Ari II flow ytometer nd 1 x 1 ells were ultured for five dys in the presene of medi, or. (,d), Cells were susequently stined nd nlyzed using BD LSR II flow ytometer for, CD49 nd CD4 on dy 5 post-ulture. Itliized numers represent MFI of CD49 stining. (e), Totl ells reovered on dy 5 post-ulture in the presene of medi, or. (f), Cytology of ells fter 5 dy ulture in the presene of medi, or ws performed. (-d, f), Results re representtive of t lest three seprte experiments. (e), Results re ompiled from three seprte experiments. Sle r, 1 μm. 4
5 SUPPLEMENTARY INFORMATION RESEARCH sophils % survivl 2 1 Medi Spleen % NBNT omprtment Csf2r2 / Csf2r x Csf2r2 / Csf2r + isotype ontrol Csf2r2 / Csf2r + nti- Bone Mrrow % NBNT omprtment 1..5 Csf2r2 / Csf2r x Csf2r2 / Csf2r + isotype ontrol Csf2r2 / Csf2r + nti- Supplementry Figure 5. nd promote mture sophil survivl. (), Bsophils were sort-purified from the lood nd spleens of mie nd ultured over-night in medi lone, or. Bsophil survivl ws mesured flow ytometrilly y exlusion of the viility dye 7-AAD. Results re ompiled from three seprte experiments. (), Spleen nd () one mrrow ells were isolted from, Csf2r2-/-/ Csf2r-/- x Tslpr-/- mie or Csfr22-/-/ Csf2r-/- mie treted with either n isotype ontrol or neutrlizing ntiody over 7 dy period. Perentges of sophils in the NBNT omprtment re illustrted. Bsophils were identified s NBNT, CD49+, Fε RI+ nd -Kit-. Results re representtive of two seprte experiments of mie per group. 5
6 RESEARCH SUPPLEMENTARY INFORMATION Monoyte nd dendriti ell mturtion (1) Mtrix metlloproteinses (2) TNFα signling () Linolei id metolism(4) Arhidoni id metolism (5) Cell ommunition (6) Cell dhesion moleules (7) Enrihment Sore Gene List (1) Gene List (2) Gene List () Gene List (4) Gene List (5) Gene List (6) Gene List (7) C1QB (1226) MRC1 (175) FABP4 (1177) CCL22 (2299) LPL (16956) PPIC (198) GATM (6792) CD74 (16149) CCL17 (2295) MMP12 (1781) IARS (15148) ACAA2 (5258) CD86 (12524) CCR7 (12775) SPP1 (275) GDF15 (2886) TRIB2 (21741) PDLIM4 (794) SLA (2491) HSD11B1 (1548) MMP1 (1786) MMP8 (1794) MMP9 (1795) MMP19 (5822) TIMP (21859) MMP12 (1781) TIMP1 (21857) TIMP2 (21858) TSPAN (5644) CCNC (5181) TGFBR (21814) PSMB8 (1691) CCL2 (2296) PRDX1 (18477) SOD2 (2656) CXCL1 (14825) ACLY (4112) RNASEN (14) SFA (7562) PSMB7 (19177) ITGB2 (16414) KIT (1659) NFKBIA (185) PLA2G (27625) ALOX15 (11687) PLA2G1 (26565) PLA2G12B (6986) PLA2G5 (18784) CYP2E1 (116) PTGDS (19215) DHRS4 (282) PLA2G1B (18778) PTGIS (1922) GPX2 (14776) GPX5 (1478) CBR (19857) PLA2G2D (18782) PTGES (64292) LTA4H (1699) PLA2G (27625) ALOX15 (11687) GPX6 (75512) PTGS2 (19225) PLA2G1 (26565) PLA2G12B (6986) PLA2G5 (18784) CYP2E1 (116) KRT9 (2794) INA (22618) KRT77 (4622) KRT17 (16667) ITGB4 (192897) COL11A1 (12814) LAMA (16774) KRT2 (94179) LAMC2 (16782) LAMB2 (16779) COL2A1 (12824) LMNB2 (1697) KRT78 (211) GJB5 (14622) COLA1 (12825) KRT6B (16688) DSC1 (155) COL6A1 (128) COL4A2 (12827) KRT72 (15866) CD6 (12511) NRXN1 (18189) CD22 (1248) MPZ (17528) CLDN (1279) PTPRC (19264) CD8 (12519) CDH4 (12561) JAM (8964) CD4 (1254) SDC2 (15529) PDCD1 (18566) PVRL2 (19294) PVRL1 (5825) ITGA4 (1641) JAM2 (6774) NRXN (18191) CLDN5 (12741) CDH5 (12562) NLGN2 (216856) Supplementry Figure 6. Heterogeneity of sophil popultions reveled y genome-wide trnsriptionl profiling. Bone mrrow ws tken from mie nd ultured in the presene of or for 5 dys. NBNT, CD49+, +, -Kit- sophils were sorted, RNA ws extrted nd smples were nlyzed y mirorry. Gene set enrihment nlysis (GSEA) ws performed with softwre ville t nd illustrted enrihment of oordinted gene sets tht orrespond to previously defined iologil proesses. List of genes for eh iologil proess numered in (Fig. 4) tht ontriute to the enrihment sore s outlined t EntrezGene ession numers for eh gene re in prenthesis. 6
7 SUPPLEMENTARY INFORMATION RESEARCH Reltive expression Supplementry 7. mpt1 mpt2 mpt5 mpt Th2 Th2 Th2 Th Reltive expression mpt7 mpt8 mpt Th2 Th2 Th2 Degrnultion β-hexosminidse (U) 2 1 mst ell ontrol mst ell sophil ontrol -eliited -eliited Supplementry Figure 7. - nd -eliited sophils exhiit phenotypi nd funtionl heterogeneity. (), Bone mrrow ws tken from mie nd ultured in the presene of or for 5 dys. NBNT, CD49+, Fε RI+, -Kit- sophils were sort-purified, nd RNA ws isolted. Th2 ells were isolted from the mesenteri LN of T. muris infeted mie on dy 21 post-infetion nd used s negtive expression ontrol. Reltive gene expression ws normlized to one mrrow-derived mst ells tht were grown in nd stem ell ftor (SCF) for 8 weeks. Rel time PCR ws performed on four iologil replites of sophils. (), Bone mrrow ws tken from mie nd ultured in the presene of or for 5 dys. NBNT, CD49+, CD2R+, -Kit- sophils were sorted nd rested for 24 hours. Bone mrrow derived mst ells were grown in the presene of nd SCF for 8 weeks. Cells were inuted with 1 μg/ml of nti-dinitrophenol (nti-dnp) IgE, wshed, nd stimulted with 1 ng/ml DNP-onjugted humn serum lumin. Superntnts were tested for β-hexosmindse y inuting with p-nitrophenyl-n-etyl-ß- D-gluosmide nd mesuring sorne t 45 nm. 7
8 RESEARCH SUPPLEMENTARY INFORMATION -eliited sophils -eliited sophils 125 IL-1α 9 IL-4 4 IL TNFα (ng/ml) CCL2 CCL CCL4 CCL CCL9.6 CCL11. CCL12 25 CCL22 (ng/ml) CXCL Supplementry Figure 8. - nd -eliited sophils exhiit differentil pilities to respond to, IL-18 nd. Bone mrrow ws tken from mie nd ultured in the presene of or for 5 dys. NBNT, CD49+, Fε RI+, -Kit- -eliited sophils (gry rs) or -eliited sophils (lk rs) were sorted nd stimulted for 12 hours with medi lone,, IL-18 or. Cell free superntnts were removed nd ssyed for () IL-1α, IL-4, IL-6, TNFα, ()CCL2, CCL, CCL4, CCL7, CCL9, CCL11, CCL12, CCL22 nd () CXCL2 y multi-nlyte pnel (Rodent v2.), ( Results from three iologil replites re shown. Sttistil nlysis ws performed using two-tiled students t test (, p<.5), (, p<.1). 8
9 SUPPLEMENTARY INFORMATION RESEARCH 2 Er 2 Serum CD49 MC9 Isotype nti %sophils /mg of tissue 1 MC9 1 MC9 Csf2r2 -/- / Csf2r -/ IgE %sophils 6 Isotype α MC9 Isotype α MC9 d e f MC9 MC CD49 BS-TRECK CD49 IL IL Bs-TRECK MC9 Bs-TRECK MC9 Supplementry Figure 9. regultes in vivo sophil responses independently of. (), mie were treted topilly on the er with ethnol () or MC9 for dys, nd lol prodution of in the er nd serum levels of were determined y ELISA. (), mie or (), Csfr22-/-/ Csf2r-/- mie were treted topilly on the er with ethnol or MC9 for four dys. NBNT, CD49+, +, -Kit- sophil responses in the er were determined fter tretment with isotype or nti- ntiody. (), results re representtive of three seprte experiments of mie per group. (,), results re representtive of two seprte experiments of mie per group. (d), mie were treted topilly on the er with ethnol or MC9 for 7 dys. (e), BS-TRECK mie were treted topilly on the er with ethnol or MC9 for 7 dys. All mie were treted with diphtheri toxin (i.p.). NBNT, CD49+, +, -Kit- sophil responses were determined on dy 7 post-mc9. (f), Drining LNs were stimulted with nti-cd nd nti-cd28 for 48 hours, nd IL-4 nd IL-5 prodution were quntified y ELISA. Results re representtive of two seprte experiments. (Ethnol n=6, MC9 n =6, Bs-TREK n=5). 9
10 RESEARCH SUPPLEMENTARY INFORMATION one mrrow lood tissue IgE-ntigen lssi sophil responses R R R R R R sophil progenitors R R IgE mture sophils effetor moleules histmine leukotrienes prostoglndins -ssoited llergi disese R R R R R R sophil progenitors IgE R R R mture sophils R R R R R R rrier surfe effetor moleules IL-4 IL-6 Cxl2 CCL12 CCL Supplementl Figure 1. Proposed model of -dependent sophil development nd funtion. (), -dependent development of lssil sophils ours in the one mrrow. promotes the prolifertion, mturtion nd susequent exit of sophils into the periphery. One in the periphery, promotes mture sophil survivl nd surfe-ound IgE is loded vi inding. In the ontext of n inflmmtory response, ntigen-speifi IgE+ sophils migrte into inflmed tissues. Antigen inds surfe-ound IgE triggering rosslinking, degrnultion nd prodution of histmines, leukotrienes nd prostglndins. () Bsophil mturtion in individuls suffering from -ssoited llergi diseses ours when inds the R nd inds the R on sophil preursors, promoting their prolifertion, mturtion nd susequent exit into the periphery. One in the periphery, sophil survivl is enhned vi -R signling, while -R signling indues surfe expression of the R. Although surfe-ound IgE is loded vi, -tivted sophils do not degrnulte in response to rosslinking. In the ontext of inflmmtion t rrier surfes, -eliited sophils migrte to inflmed tissue where they enounter, triggering their exggerted prodution of IL-4, IL-6, CxCl2, CCL12 nd CCL. 1
Supplementary Figure S1
Supplementry Figure S1 - UTR m - 3HA - 2-1 hgh - 1 Uiquitin *! *! lk distl promoter m K3R/ K121R-3HA UTR hgh founder lines - HA - - founder lines TG- E1 L A2 B1 F9 G6 H4 H6 B C D2 G1 H3 J2 L - 7 IP: lk
RESEARCH ARTICLE. Supplemental Figure 5
11.5 2 2 11. RESEARCH ARTICLE RBC ( 1 12 /L) 1.5 1. 9.5 PLT ( 1 9 /L) 1 16 14 HGB (g/l) 19 1 17 16 9. 12 4 4 46 Cellulr & Moleulr Immunology dvne online pulition, PCV (%) 44 MCV (fl) 46 44 ; doi:1.13/mi.214.16
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SUPPLEMENTARY INFORMATION doi:.8/nture89 4 4 Ilr -/- Ilr -/- Ilr -/- Cspse- -/- As -/- Nlrp -/- Il8 -/- Ilr -/- Supplementl figure. Inresed severity of NASH in inflmmsome-defiient mie, ut not in Ilr-defiient
(% of adherent cells) *** PBL firm adhesion. Frequency (% ) 4 1 L 2 CXCR3 DP-2
Chemotxis (% of dded ells) PBL totl dhesion (N ells/mm 2 /1.1 6 PBL) Frequeny (% ) PBL firm dhesion Supplementry Figure 1 4 4 3 3 2 2 1.1-4 1-3 1.1.2. 1 1 8 6 4 2 Adiponetin ( g/ml) - + Adiponetin ( g/ml)
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doi: 1.138/nture862 humn hr. 21q MRPL39 murine Chr.16 Mrpl39 Dyrk1A Runx1 murine Chr. 17 ZNF295 Ets2 Znf295 murine Chr. 1 COL18A1 -/- lot: nti-dscr1 IgG hevy hin DSCR1 DSCR1 expression reltive to hevy
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Alimonti_Supplementry Figure 1 hy 3 4 5 3 Neo 4 5 5 Proe 5 Proe hy/ hy/ /- - 3 6 Neo β-tin d Reltive Protein level (% ) 15 1 5 hy/ /- Reltive Gene Expr. (% ) 15 1 5 hy/ /- Supplementry Figure 1 Chrteriztion
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{ OI: 1.138/n31 Srifie n nlyze APs on week 1 s of iet 1 4 6 High-ft iet BrU High-ft iet BrU 4 High-ft iet BrU 6 High-ft iet BrU Lin - Lin - : C34 + : C9 + 1 1 3 1 4 1 5 C45 1 C34 1 1 1 1 3 1 4 1 5 S-1
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Supplementry figure 1 Dy 8 post LCMV infection Vsculr Assoc. Prenchym Dy 3 post LCMV infection 1 5 6.7.29 1 4 1 3 1 2 88.9 4.16 1 2 1 3 1 4 1 5 1 5 1.59 5.97 1 4 1 3 1 2 21.4 71 1 2 1 3 1 4 1 5 1 5.59.22
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The Amerin Soiety of Gene Therpy originl rtile Overoming Immune Tolerne Aginst Multiple Myelom With Lentivirl Clnexin-engineered Dendriti Cells Shuhong Hn 1, Bei Wng 1, Mtthew J Cotter 1, Li-Jun Yng 2,
DOI: 10.1038/nc2331 PCre;Ros26R 12 h induction 48 h induction Vegfr3 i EC c d ib4 24 h induction VEGFR3 e Fold chnge 1.0 0.5 P < 0.05 Vegfr3 i EC Vegfr3 Figure S1 Cre ctivtion leds to genetic deletion
Supplementary Figure 1
doi: 1.138/nture6188 SUPPLEMENTARY INFORMATION Supplementry Figure 1 c CFU-F colonies per 1 5 stroml cells 14 12 1 8 6 4 2 Mtrigel plug Neg. MCF7/Rs MDA-MB-231 * * MCF7/Rs-Lung MDA-MB-231-Lung MCF7/Rs-Kidney
... Activated T cells regulate bone loss and joint destruction in adjuvant arthritis through osteoprotegerin ligand. immunology letters to nature
Supplementry informtion is ville in Nture s World-Wide We site (http:// www.nture.om) or s pper opy from the London editoril offie of Nture. Aknowledgements Supported in prt y grnts from the NIH (A.A.,
Supplementary Figure 1
Roles of endoplsmic reticulum stress-medited poptosis in -polrized mcrophges during mycocteril infections Supplementry informtion Yun-Ji Lim, Min-Hee Yi, Ji-Ae Choi, Jung-hwn Lee, Ji-Ye Hn, Sung-Hee Jo,
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SUPPEMENTARY INFORMATION DOI: 1.138/ncb956 Norml CIS Invsive crcinom 4 months months b Bldder #1 Bldder # Bldder #3 6 months (Invsive crcinom) Supplementry Figure 1 Mouse model of bldder cncer. () Schemtic
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doi: 10.1038/nture07679 Emryonic Stem (ES) cell Hemngiolst Flk1 + Blst Colony 3 to 3.5 Dys 3-4 Dys ES differentition Sort of Flk1 + cells Supplementry Figure 1. Chrcteristion of lst colony development.
Regulatory T cells prevent catastrophic autoimmunity throughout the lifespan of mice
Regultory T ells prevent tstrophi utoimmunity throughout the lifespn of mie Jeong M Kim 1, Jeffrey P Rsmussen 1 & Alexnder Y Rudensky 1,2 Mie lking the trnsription ftor ( ) lk regultory T (T reg ) ells
Inhibitory effect of p38 mitogen-activated protein kinase inhibitors on cytokine release from human macrophages
British Journl of Phrmology (26) 149, 393 44 & 26 Nture Pulishing Group All rights reserved 7 1188/6 $3. www.rjphrmol.org RESEARCH PAPER Inhiitory effet of p38 mitogen-tivted protein kinse inhiitors on
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doi:.38/nture99 Dy Dy Dy 3 Dy 4 Bleed INDO INDO INDO INDO INDO INDO INDO INDO 3 7 CFU-GM/ml lood 6 4 3 Indomethcin Biclein + Indo + Bcln c WBC PMN LYMPH MONO RBC PLT Ctrl 7.4±.9.4±.8.4±.39.37±.6 8.88±.
Identification of TROP2 (TACSTD2), an EpCAM-Like Molecule, as a Specific Marker for TGF-b1-Dependent Human Epidermal Langerhans Cells
ORIGINAL ARTICLE Identifition of (TACSTD2), n -Like Moleule, s Speifi Mrker for TGF-1-Dependent Humn Epiderml Lngerhns Cells Gregor Eisenwort 1, Jennifer Jurkin 1, Night Ysmin 1, Thoms Buer 1, Bernhrd
Toll-Like Receptor Activation during Cutaneous Allergen Sensitization Blocks Development of Asthma through IFN-Gamma-Dependent Mechanisms
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Anti-Inflammatory Activity of Methanol Extract and Fractions from Alchemilla kiwuensis Engl. on LPS Activated Macrophages
Aville online on www.ijppr.om Interntionl Journl of Phrmognosy nd Phytohemil Reserh 217; 9(4); 473-481 DOI numer: 1.25258/phyto.v9i2.8117 Reserh Artile ISSN: 975-4873 Anti-Inflmmtory Ativity of Methnol
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DOI:./n BJ RAS:ER Herrnz et l Supplementry Figure HFFF RAS:ER.. mrna Expression..... ILα ILβ IL IL CCL INH VEGF mrna Expression..... ILα ILβ IL IL CCL INH VEGF + OHT Torin NVP-BEZ + OHT shmtor. shmtor.
Supplemental Figures and Legends
Supplementl Figures n Legens Epigeneti trgeting of Hegehog trnsriptionl output through BET romoomin inhiition Yujie Tng 1,2, Shrreh Gholmin 2, Simone Shuert 1,2, Mine I. Willrson 3, Alex Lee 4, Prtiti
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doi:10.1038/nture09663 Scrmle shnlrp3 shcsp1 IL-1β (p17) IL-1β (pg/ml) 2000 1500 1000 500 Wt Nlrp3-/- Ipf-/- 0 APDC IL-1β (p17) Supplementl Figure 1. Mitochondril ROS cn trigger NLRP3 inflmmsome ctivtion,
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doi:1.138/nture17 Men tumour dimeter (mm) 2 Rg2-/- 2 1 2 2 1 Control IgG!-CD8!-CD4 1 2 3 1 2 3 c Men tumour dimeter (mm) 2 2 1 d Ifnr1-/- Rg2-/- 2 2 1 Ifngr1-/- d42m1!ic 1 2 3 Dys post trnsplnt 1 2 3 Supplementry
Origin of Triple hi and Triple lo T reg cells Triple hi and Triple lo T reg cells present in the thymus (Fig. 2a) could represent CD4 + GITR CD4 PD-1
Affinity for self ntigen selets with distint funtionl properties Len Wyss 1,2, Brin D Stdinski 3, Crolyn G King 1, Sonj Shllenerg 4, Nihols I MCrthy, Jun Young Lee 6,7, Krsten Kretshmer 4,8, Luigi M Terrino
Thioredoxin-interacting protein links oxidative stress to inflammasome activation
A rt i l e s Thioredoxin-interting protein links oxidtive stress to inflmmsome tivtion Rongin Zhou 1, Aury Trdivel 1, Bernrd Thorens 2, Inpyo Choi 3 & Jürg Tshopp 1 29 Nture Ameri, In. All rights reserved.
Ulk λ PPase. 32 P-Ulk1 32 P-GST-TSC2. Ulk1 GST (TSC2) : Ha-Ulk1 : AMPK. WB: Ha (Ulk1) : Glu. h CON - Glu - A.A WB: LC3 AMPK-WT AMPK-DKO
DOI: 10.1038/ncb2152 C.C + - + - : Glu b Ulk1 - - + λ PPse c AMPK + - + + : ATP P-GST-TSC2 WB: Flg (Ulk1) WB Ulk1 WB: H (Ulk1) GST (TSC2) C.C d e WT K46R - + - + : H-Ulk1 : AMPK - + - + + + AMPK H-Ulk1
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SUPPLEMENTARY INFORMATION doi:1.138/nture1188 1mM CCl 2 (min) 3 4 6 CCl 2 (mm) for 4min.1. 1 (mm) Pro- d WT GdCl 3 R-68 -/- P2x7r -/- -/- Csp1 -/- WT -/- P2x7r -/- -/- Csp1 -/- Csp1 (p2) (p17) Pro-Csp1
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SUPPLEMENTARY INFORMATION doi: 1.138/nnno.211.41 Sili nd titnium dioxide nnoprtiles use pregnny omplitions in mie Kohei Ymshit, Ysuo Yoshiok, Kzum Higshisk, Kzuy Mimur, Yuki Morishit, Mstoshi Nozki, Tokuyuki
Essential role of NKT cells producing IL-4 and IL-13 in the development of allergen-induced airway hyperreactivity
Essentil role of NKT ells produing IL-4 nd IL-13 in the development of llergen-indued irwy hyperretivity OMID AKBARI 1, PHILIPPE STOCK 1, EVERETT MEYER 1, MITCHELL KRONENBERG 2, STEPHANE SIDOBRE 2, TOSHINORI
Intestinal epithelial tuft cells initiate type 2 mucosal immunity to helminth parasites. Naive. Naive. Naive
doi:1.138/nture16527 Intestinl epithelil tuft ells initite type 2 muosl immunity to helminth prsites Frnçois Gere 1,2,3, Emmnuelle Sidot 1,2,3, Dnielle J. Smyth 4, Mkoto Ohmoto 5, Ihiro Mtsumoto 5, Vlérie
TNF-a Downregulates Filaggrin and Loricrin through c-jun N-terminal Kinase: Role for TNF-a Antagonists to Improve Skin Barrier
ORIGINAL ARTICLE TNF- Downregultes Filggrin nd Loririn through -Jun N-terminl Kinse: Role for TNF- Antgonists to Improve Skin Brrier Byung Eui Kim, Mihel D. Howell,, Emm Guttmn,, Ptrii M. Gilleudeu, Irm
% cells forming Neurospheres 81 ± 6 % 0 % 2.6 ± 0.7 % 76 ± 8 % 0 % 3.4 ± 0.6 % 83 ± 5 % 0 % 2.4 ± 0.9 % 89 ± 5 % 3 ± 1.5 % Total 10, ± 6 % 0 %
Bo et l., Suppl. Tle 1 Supplementl Tle 1. Neurosphere formtion nd tumorigencity is enriched within the tumour cell popultions derived from humn primry glioms nd gliom xenogrfts. GBM smples or Gliom xenogrfts
nestin ironetin p75 s1 CNS SKPs Dermo-1 +ve SKPs CNS H2O SCGs Skin Di. SKPs TH SHOX2 GAPDH NCAM D H Figure S1, Immunoytohemil nlysis o SKP spheres ulture rom neontl mouse (nestin, ironetin, S-1) or rt
AMPK maintains energy homeostasis and survival in cancer cells via. regulating p38/pgc-1α-mediated mitochondrial biogenesis
SUPPLEMENTARY INFORMATION AMPK mintins energy homeostsis nd survivl in cncer cells vi regulting p38/pgc-1α-medited mitochondril iogenesis Blkrishn Chue 1, Prmnnd Mlvi 1, Shivendr Vikrm Singh 1, Noshd Mohmmd
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doi:0.08/nture078 RNse VifHA VifHA βctin 6 Cell lyste IP: ntiha MG VifHA VifHA β ctin 6 7 Cell lyste IP: ntiha Supplementry Figure. Effect of RNse nd MG tretment on the Vif interction., RNse tretment does
Cytomegalovirus and tumor stress-surveillance by human γδ T cell receptor binding to Endothelial Protein C Receptor
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Icos-/ CD3 Icos Y181F OT-2 T cells doi:1.138/nture158 IgD Supplementry Figure 1. is required for folliculr locliztion of ctivted helper T cells. Icos nd Icos-/- OT-2 T cells were retrovirlly trnsduced
Erucin Exerts Anti-Inflammatory Properties in Murine Macrophages and Mouse Skin: Possible Mediation through the Inhibition of NFκB Signaling
Int. J. Mol. Si. 213, 14, 2564-2577; doi:1.339/ijms1412564 Artile OPEN ACCESS Interntionl Journl of Moleulr Sienes ISSN 1422-67 www.mdpi.om/journl/ijms Eruin Exerts Anti-Inflmmtory Properties in Murine
Capsid-specific T-cell Responses to Natural Infections With Adeno-associated Viruses in Humans Differ From Those of Nonhuman Primates
originl rtile See pge 1923 Cpsi-speifi T-ell Responses to Nturl Infetions With Aeno-ssoite Viruses in Humns Differ From Those of Nonhumn Primtes Hu Li 1, Mrio O Lsro 1, Bei Ji 1,2, Shih Wen Lin 1,3, Lriss
EFFECTS OF AN ACUTE ENTERIC DISEASE CHALLENGE ON IGF-1 AND IGFBP-3 GENE EXPRESSION IN PORCINE SKELETAL MUSCLE
Swine Dy 22 Contents EFFECTS OF AN ACUTE ENTERIC DISEASE CHALLENGE ON IGF-1 AND IGFBP-3 GENE EXPRESSION IN PORCINE SKELETAL MUSCLE B. J. Johnson, J. P. Kyser, J. D. Dunn, A. T. Wyln, S. S. Dritz 1, J.
Bacterial Pili exploit integrin machinery to promote immune activation and efficient blood-brain barrier penetration
Reeived Jun Aepted Aug Pulished 6 Sep DOI:.8/nomms7 Bteril Pili exploit integrin mhinery to promote immune tivtion nd effiient lood-rin rrier penetrtion Anirn Bnerjee, Brndon J. Kim,, Ellese M. Crmon,,
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DOI:.38/nc393 Nnog DAPI Nnog/DAPI c-jun DAPI c-jun/dapi c e Reltive expression to Gpdh mescs ( Feeder free) mescs ( Feeder) MEFs.5 MEFs ipscs ESCs..5 p=.24 p=.483 p=.22. JunB JunD Fos Fr Fr2 ATF2 ATF3
PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis
Supplementry Informtion PDGF-BB secreted y preosteoclsts induces ngiogenesis during coupling with osteogenesis Hui Xie, Zhung Cui, Long Wng, Zhuying Xi, Yin Hu, Lingling Xin, Chngjun Li, Ling Xie, Jnet
BTLA is a lymphocyte inhibitory receptor with similarities to CTLA-4 and PD-1
BTLA is lymphoyte inhiitory reeptor with similrities to CTLA-4 nd PD-1 Norihiko Wtne 1,5, My Gvrieli 1, John R Sedy 1, Jinfei Yng 1,5, Frnes Fllrino 2, Susn K Loftin 1, Mihelle A Hurhl 1, Ntlie Zimmermn
Supplementary Figure S1
Supplementry Figure S1 d MAP2 GFAP e MAP2 GFAP GFAP c f Clindin GFAP Supplementry Figure S1. Neuronl deth nd ltered strocytes in the rin of n ffected child. Neuron specific MAP2 ntiody stining in the hippocmpus
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SUPPLEMEARY IFORMAIO doi:./nture correction to Supplementry Informtion Adenom-linked rrier defects nd microil products drive IL-/IL-7-medited tumour growth Sergei I. Grivennikov, Kepeng Wng, Dniel Mucid,
Supplementary Information. SAMHD1 Restricts HIV-1 Infection in Resting CD4 + T Cells
Supplementry Informtion SAMHD Restricts HIV- Infection in Resting CD T Cells Hnn-Mri Blduf,2,, Xioyu Pn,, Elin Erikson,2, Srh Schmidt, Wqo Dddch 3, Mnj Burggrf, Kristin Schenkov, In Amiel,2, Guido Wnitz
Activation of Akt as a Mechanism for Tumor Immune Evasion
The Amerin Soiety of Gene Therpy originl rtile Ativtion of Akt s Mehnism for Tumor Immune Evsion Kyung Hee Noh 1, Te Heung Kng 1, Jin Hee Kim 1, Sr I Pi 2, Ken Y Lin 3, Chien-Fu Hung 4, T-C Wu 4 7 nd Te
ARTICLES. Mediators of vascular remodelling co-opted for sequential steps in lung metastasis
Vol 1 April 7 doi:1.13/nture57 ARTICLES Meditors of vsulr remodelling o-opted for sequentil steps in lung metstsis Gorv P. Gupt 1, Don X. Nguyen 1, Anne C. Ching 1,, Pul D. Bos 1, Juliet Y. Kim 1, Cristin
a3 Chains of type V collagen regulate breast tumour growth via glypican-1
Reeive 5 Aug 16 Aepte De 16 Pulishe 19 Jn 17 3 Chins of type V ollgen regulte rest tumour growth vi glypin-1 Guorui Hung 1, Goxing Ge 1,w, Vlerio Izzi & Dniel S. Greenspn 1 DOI: 1.138/nomms1351 OPEN Periellulr
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doi:10.1038/nture09973 Plsm Memrne Phgosome TLR1/2/4 ROS Mitochondrion ROS OXPHOS Complex I ROS TRAF6 NADPH Oxidse Supplementry Figure 1 Model detiling the roles of mitochondril ROS in mcrophge cteril
Unidirectional transfer of microrna-loaded exosomes from T cells to antigen-presenting cells
Reeived 9 Aug Aepted Mr Pulished 9 Apr DOI:.8/nomms8 Unidiretionl trnsfer of mirorna-loded exosomes from T ells to ntigen-presenting ells Mrí Mittelrunn,, Cristin Gutiérrez-Vázquez,, Crolin Villrroy-Beltri,
Type II monocytes modulate T cell-mediated central nervous system autoimmunity
Type II monocytes modulte T cell-medited centrl nervous system utoimmunity Mrtin S. Weer, Thoms Prod homme, Swsn Youssef, Shnnon E. Dunn, Cynthi D. Rundle, Lind Lee, Jun C. Ptrroyo, Olf Stüve, Rymond A.
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doi:0.08/nture0987 SUPPLEMENTARY FIGURE Structure of rbbit Xist gene. Exons re shown in boxes with romn numbers, introns in thin lines. Arrows indicte the locliztion of primers used for mplifiction. WWW.NATURE.COM/NATURE
YAP transcriptionally regulates COX-2 expression and GCCSysm-4 (G-4), a dual YAP/COX-2 inhibitor, overcomes drug resistance in colorectal cancer
Li et l. Journl of Experimentl & Clinil Cner Reserh (7) 36:44 DOI.86/s346-7-6-3 RESEARCH Open Aess trnsriptionlly regultes expression nd GCCSysm-4 (G-4), dul / inhiitor, overomes drug resistne in oloretl
DNA released from dying host cells mediates aluminum adjuvant activity
DNA relesed from dying host cells medites luminum djuvnt ctivity Thoms Mrichl 1, Keiichi Oht 2, Denis Bedoret 1, Clire Mesnil 1, Ctherine Stel 1, Kouji Koiym 2,3, Pierre Lekeux 1, Cevyir Con 2, Shizuo
Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone
Reeived 2 De 215 Aepted 9 Nov 216 Pulished 14 De 216 DOI: 1.138/nomms13885 OPEN Osteolsts serete to regulte ngiogenesis in one Bin Hung 1,, Wenho Wng 1,, Qinghu Li 1,, Zhenyu Wng 1,BoYn 1, Zhongmin Zhng
well for 2 h at rt. Each dot represents an individual mouse and bar is the mean ±
Supplementary data: Control DC Blimp-1 ko DC 8 6 4 2-2 IL-1β p=.5 medium 8 6 4 2 IL-2 Medium p=.16 8 6 4 2 IL-6 medium p=.3 5 4 3 2 1-1 medium IL-1 n.s. 25 2 15 1 5 IL-12(p7) p=.15 5 IFNγ p=.65 4 3 2 1
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DOI: 1.138/nc286 Figure S1 e f Medium DMSO AktVIII PP242 Rp S6K1-I Gr1 + + + + + + Strvtion + + + + + IB: Akt-pT38 IB: Akt K-pT389 K IB: Rptor Gr1 shs6k1-a shs6k1-b shs6k1-c shrictor shrptor Gr1 c IB:
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1 SUPPLEMENARY INFORMAION 13 supplementry figures, 2 supplementry tles, nd supplementry note he leukocyte integrin ntgonist Del-1 inhiits IL-17 medited inflmmtory one loss Mehmet A. Eskn 1,2, Rvi Jotwni
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X p -lu c ct ivi ty doi:.8/nture8 S CsA - THA + DAPI Merge FSK THA TUN Supplementry Figure : A. Ad-Xp luc ctivity in primry heptocytes exposed to FSK, THA, or TUN s indicted. Luciferse ctivity normlized
Type I Interferons Interfere with the Capacity of mrna Lipoplex Vaccines to Elicit Cytolytic T Cell Responses
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. Norml Physiologicl Conditions. SIRT1 Loss-of-Function S1. Model for the role of SIRT1 in the regultion of memory nd plsticity. () Our findings suggest tht SIRT1 normlly functions in coopertion with YY1,
original article Received 7 November 2008; accepted 2 March 2009; published online 24 March doi: /mt
The Amerin Soiety of Gene Therpy originl rtile Immuniztion With Adenovirusvetored Tuerulosis Vine Provides Mrkedly Improved Protetion Over Its Counterprt Aginst Pulmonry Tuerulosis Jingyu Mu 1, Mnglkumri
Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization
8 Nture Pulishing Group http://www.nture.om/ntureimmunology rystls nd luminum slts tivte the NALP3 inflmmsome through phgosoml destiliztion Veit Hornung 1,, Frnz Buernfeind,, Annett Hlle 1, Eivind O Smstd
Insulin-like Growth Factor-binding Protein-7 (IGFBP7): A Promising Gene Therapeutic for Hepatocellular Carcinoma (HCC)
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Supplementary Figure S1
Supplementry Figure S Connexin4 TroponinI Merge Plsm memrne Met Intrcellulr Met Supplementry Figure S H9c rt crdiomyolsts cell line. () Immunofluorescence of crdic mrkers: Connexin4 (green) nd TroponinI
NappHS. rrna. transcript abundance. NappHS relative con W+W 0.8. nicotine [µg mg -1 FM]
![NappHS. rrna. transcript abundance. NappHS relative con W+W 0.8. nicotine [µg mg -1 FM]](/thumbs/92/110177253.jpg "NappHS. rrna. transcript abundance. NappHS relative con W+W 0.8. nicotine [µg mg -1 FM]")
(A) W+OS 3 min 6 min con L S L S RNA loding control NppHS rrna (B) (C) 8 1 k NppHS reltive trnscript undnce 6 4.5 *** *** *** *** 3 k. + + + line 1 line (D) nicotine [µg mg -1 FM] 1..8.4. con W+W Supplementl
Evaluation of humoral immune status in porcine epidemic diarrhea virus (PEDV) infected sows under. field conditions
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