SUPPLEMENTARY INFORMATION
|
|
- Joseph McKenzie
- 5 years ago
- Views:
Transcription
1 DOI:./n BJ RAS:ER Herrnz et l Supplementry Figure HFFF RAS:ER.. mrna Expression..... ILα ILβ IL IL CCL INH VEGF mrna Expression..... ILα ILβ IL IL CCL INH VEGF + OHT Torin NVP-BEZ + OHT shmtor. shmtor. mtor ILα ILα ILβ ILβ IL IL pebp T/T ps S/S pebp T/T ps S/S.. Intensity (.u.).... mrna Expression pebp T/T ps S/S mtor. mtor Intensity (.u.)..... IL IL! IL" Downregulte ftors Upregulte ftors Torin NVP-BEZ Common rugs 7 Common rugs 9 9 shmtor shmtor shmtor shmtor shmtor Supplementry Figure mtor inhiition regultes the SASP.. mtor inhiition regultes the SASP in ifferent firolsts strins. BJ ER:RAS n HFFF ER:RAS ells were generte n inue with OHT to tivte ER:RAS in the presene of nm rpmyin, nm Torin or. nm NVP-BEZ. Seven ys fter OHT inution, RNA ws ollete n qpcrs performe to etet the expression of selete SASP omponents. Dt re men ± s.. from n= inepenent experiments. Stuent s T-test ws performe to ompre the ifferent tretments to the ontrol (R+) with P <.. V, ells expressing n empty ; R, ells infete with plnc-er:ras.,. mtor inhiition () or mtor knokown () ereses phosphoryltion of S (ps S/S ) n eifebp (pebp T/T ) proteins n inhiits the inution of the SASP. Inite immunostinings were performe 7 ys fter OHT inution. Fol hnges of normlise vlues n representtive imges re shown. Error rs represent the ± s.. from n= inepenent experiments. Stuent s T-test ws performe to ompre trete/shmtor ells to the ontrol senesent ells. P <.. Sle r, mm.. Venn igrms representing the overlp n signifine for the SASP omponents ownregulte or upregulte y multiple mtor inhiitors n mtor knokown. The signifine of overlp ws ompute using hypergeometri test. P <.; P <.. For rw t, see Supplementry Tle 7. Mmilln Pulishers Limite. All rights reserve
2 . p INK p CIP Supplementry Figure Intensity (.u.).... V+ R+ R+ R+Torin R+NVP-BEZ Intensity % Positive (.u.) Cells. pink SA-β-Gl % Positive ells CDKNA BrU Intensity (.u.) p INK pink Dy p CIP CDKNA Dy - OHT Torin NVP-BEZ Torin NVP-BEZ OHT) Torin NVP-BEZ OHT CylinD) GAPDH) IMR9 ER:RAS e f Torin OHT 7 9 Torin +OHT, ps S/S, S, pebp T/T, EBP, GAPDH, Intensity (.u.) Intensity (.u.) IL IL! IL" pebp T/T ps S/S V+ R+ R+Torin Supplementry Figure mtor inhiition ontrols the senesene phenotype n ell prolifertion.. mtor inhiition ereses the levels of ell yle inhiitors involve in senesene. p INK n p CIP levels were mesure in the sme experimentl settings s in Supplementry Fig.. Error rs represent the ± s.. from n= inepenent experiments.. mtor knokown results in erese SA--Gl tivity, p INK n p CIP levels ut ells remin rreste. BrU inorportion n senesene mrkers were etermine fter 7 ys of inution with OHT. Dt re men ± s.. from n= (BrU) n n= (SA--Gl, p n p) inepenent experiments. Stuent s T-test ws performe to ompre the shmtor ells with the senesent ontrols (shct+oht). P <.; P <.: n.s, non signifint.. mtor inhiition ereses prolifertion of norml IMR9 humn firolsts n oes not resue OIS rrest. IMR9 ER:RAS ells ( x ) were trete s inite n prolifertion ws ssesse y CV stining fter ys in ulture. Imges re representtive of inepenent experiments.. Cylin D expression levels were ssesse y immunolot. IMR9 ER:RAS ells were left untrete or trete with OHT n the inite rugs for or ys. Dt re representtive of n= inepenent experiments. e-f. These figures re n extension of the experiment presente in Fig. -. Torin inhiition of mtor ownstrem trgets (ps S/S, pebp T/T ) n the SASP ws ssesse y immunolot (e) n IF (f) 7 ys fter OHT inution. Immunolots re representtive of n= inepenent experiments. Error rs represent the ± s.. from n= inepenent experiments. Stuent s T-test ws performe to ompre Torin trete ells to the ontrol. P <.. Unproesse originl sns of lots re shown in Supplementry Fig. 9. For rw t, see Supplementry Tle 7. Mmilln Pulishers Limite. All rights reserve
3 +OHT EBP DN 7 Supplementry Figure EBP p-ebp T/ Intensity (.u.) EBP-DN. EBP pebp T/T mrna Expression Vetor EBP-DN Mok Gy Irrition Gy Irrition IL IL CCL ILβ IMR9 ER:RAS + OHT O.D. O.D. Torin Seimenttion O.D..... S S Mono Polysomes S 7 9 Frtion numer % EEF mrna % EEF mrna.%.% 7 9 Frtions Torin 9.% 7.7% 7 9 Frtions e % mrna in Polysomes InhiinA MMP Torin Torin MMP MMP Torin Torin Supplementry Figure Effet of mtor n EBP on the trnsltion of SASP omponents.. This is n extension of Figure -. Expression of n eifebp phospho-mutnt (EBP DN) results in erese phosphorylte EBP Thr/ levels. (right) Fol hnges of normlise intensity vlues otine from EBP n EBP Thr/ immunostinings Dt re men ± s.. from n= inepenent experiments. (left) Imges from representtive experiment re shown. Dt re men ± s.. from n= inepenent experiments. For ll grphs, stuent s T-test ws performe to ompre ells expressing EBP DN to the ontrol (+ ). P <.. Sle r, mm.. Expression of EBP DN impirs the SASP levels oserve in irrite IMR9 firolsts. RNA ws ollete ys fter irrition ( Gy). Dt re men ± s.. from n= inepenent experiments. Stuent s T-test ws performe to ompre the ells expressing EBP-DN to the ontrol ( irrite). P <.; P <.. Profile of mtor-regulte trnsltion in OIS. Representtive polysome profiles of IMR9 ER:RAS ells (inute for ys with OHT) trete with or nm Torin for h from n= inepenent experiments.. A shemti representtion of the position of frtions ( ), monosomes n polysomes is shown together with representtive profile for EEF mrna ( nonil mtor trget). Dt re representtive of inepenent experiments. e Polysome nlysis of mrnas for SASP omponents. Grphs show the perentge of the inite mrnas in tively trnslting polysomes uring OIS. Error rs represent the ± s.. from n= inepenent experiments. Stuent s T-test ws performe to ompre Torin trete ells to the -trete ontrols. P <.; P <.; P <.; N.S, non signifint. For rw t, see Supplementry Tle 7. Mmilln Pulishers Limite. All rights reserve
4 S U P P L E M E N TA R Y I N F O R M AT I O N Herrnz et l Supplementry Figure + + Control Torin To rin IMR9&ER:RAS& BJs$ER:RAS$ IMR9&ER:RAS& ER:RAS inution (ys) BJ#ER:RAS# ER:RAS inution (ys) Dys% D pmapkapkt& MAPKAPK& MAPKAPK& MAPKAPK& pakts7& pakts7& pakts7& pakts& pakts& pakts& AKT& AKT& AKT& pss/s& pss/s& pss/s& S& S& S& pebpt/t& pebpt/t& pebpt/t& EBP& EBP& EBP& GAPDH&.. Dys mrna Expression D D D D D D Dys MK MK ps ps pebp pebp reltive intensity (.u.). HFFF ER:RAS MK ps pebp D GAPDH& HFFF ER:RAS HFFF ER:RAS. D D D D D D MK ps pebp D Dys D D D shct(-oht) shct(+oht) shmk. shmk... MK ps pebp.. Dys% IL IL ILα ILβ CCL Supplementry Figure MAPKAKP regultes the SASP.. Polysome istriution of MKK mrna. Error rs represent the ± s.. from n= inepenent experiments. Stuent s T-test ws performe to ompre Torin trete ells to the ontrol., non signifint.. Relte to Fig e. Smples were immunolotte with Streptviin-HRP to etet AHA-Biotin ontining proteins. Smples purifie y pull own with streptviin es (Strept. PD) were nlyse. Dt re representtive of inepenent experiments.. MAPKAPK protein levels orrelte with mtor signlling. IMR9 ER:RAS, BJ ER:RAS n HFFF ER:RAS ells were left untrete or trete with OHT for the inite times. (Top) Immunolots were performe with the inite ntioies. Dt re representtive of inepenent experiments. INHBA VEGFC shmk.' pmapkapkt& Reltive intensity (.u.) pmapkapkt& reltive intensity (.u.) Reltive intensity (.u.) reltive intensity (.u.) ZFPL&. ZFPL& GAPDH& ZFPL& HFFF ER:RAS Dys% shmk.' shctrl' HFFF$ER:RAS$ ER:RAS inution (ys) HFFF$ER:RAS$ Reltive intensity (.u.) Co nt ro l % mrna in Polysomes + Streptviin-HRP % mrna in Polysomes - CHX AHA-Biotin MKK Torin Strept. PD MAPKAPK' GAPDH' MK (Bottom) Quntifition of MK, pss/s n pebpt/t lots. Error rs represent the ± s.. from n= inepenent experiments.. MAPKAPK inhiition prevents SASP inution. IMR9 ER:RAS ells were infete with the inite hirpins n further trete with OHT. RNA n protein extrts were ollete t y fter inution. Expression of the SASP n MK were ssesse y qrt-pcr (left) or immunolotting (right). Immunolot imges re representtive of inepenent experiments. Error rs represent the ± s.. from n= inepenent experiments. Stuent s T-test ws performe to ompre ells efiient for MK to ontrol ells (shct + OHT ). P <.; P <.; P <.; N.S, non signifint. Unproesse originl sns of lots re shown in Supplementry Fig. 9. For rw t, see Supplementry Tle 7. Mmilln Pulishers Limite. All rights reserve
5 e g Averge Pek Are ZPL Ative: - Degre ARE mrnas - Unstle protein pzfpl Ser Rp + OHT RAS GV p-mk j S S9 S ZPL S! S9! S! ZFPL FPRRHSVTLPS RDRSFSEGGER LQHSFSFAGFP ZFPL FLRRHSASNLH RDRSFSENGDR LHHSLSFSGFP S7! S! S9! Intive: INACTIVE - Stilize y ining to -- S! S9! S! ZFPL FPRRHSVTLPS ----FSEGGER LQHSFSFAGFP TTP WGSSGPWSLSP PGFAPLAPRLG LRQSISFSGLP S! S! S7! mmttp TGRSTSLVEGR LRQSISFSGLP http PGRSTSLVEGR LRQSISFSGLP >> TTP Mut (SA,S S! S! h Vetor Chrterize+MK+ phosposites+ ZFPL WT MUT TTP Vetor WT MUT ZFPL GAPDH TTP GAPDH i -- ZFPL (long exp.) p-hsp7 S HSP7 GAPDH SASP omponents own in ll onitions NTD BOLA CCL CCL CSF CSF CXCL ERAP GROA OHT!!"! +! +! +! +! ZFPL (short exp.) p-zfpl A A9 A ZPL Mut Constitutively tive - Degre ARE mrnas - Cnnot e intivte y MK - Highly Unstle IL IL IL INHBA NAMPT POSTN TFPI TSP f l Density Herrnz et l Supplementry Figure MK inh. III.... k mtor inh. Up % mrna in Polyosome p u p u. Men ARE Sore ZFPL Control Upregulte ftors ZFPL Mut mtor inh Density Common Down in mtor inh. & ZFPL Mut..... Torin Vetor ZFPL Mut IL IL CCL ILβ IL IL ILα ILβ CCL INHBA Mok Gy Irrition Gy Irrition EV - OHT EV + OHT ZFPL WT ZFPL MUT ZFPL WT ZFPL MUT TTP WT TTP MUT Men ARE Sore Supplementry Figure ZFPL regultes the SASP.. Serine in ZFPL ws ientifie s n mtor inhiition-sensitive phosphosite y phosphoproteomis (n=).. Extension of Fig. Speifiity of MK inhiitor III is prove y the erese in the phosphoryltion of HSP7 (phsp7 S ) ownstrem trget of MK.. Shemti moel of ZFPL tivity n protein stility.. Perentge of ZFPL mrna in trnslting polysomes uring OIS (n=) e. IMR9 ER:RAS ells were infete s inite. Immunostinings were performe 7 ys fter OHT inution. Fol hnges of normlise intensity vlues re shown (n=). f. SASP genes expression ws mesure in IMR9 firolsts ys fter irrition ( Gy) (n=). g. Designe TTP n ZFPL phosphomutnts. Sine MK phosphosites for TTP n ZFPL hve not een esrie, we ligne their sequenes with tht of ZFPL iming to ientify puttive MK phosphosites. In the se of TTP, homology is low. However, the two known MK phosphosites in the murine form of TTP 9 re onserve in the humn form. h-i. IMR9 ER:RAS ells were infete with ZFPL wt/mut, ZFPL wt/mut or TTP wt/mut. OIS ws inue y OHT for 7 ys. (h) Protein levels were nlyse y immunolot in presene of MG ( mm, h). (i) Expression of SASP genes ws mesure y qrt-pcr (n=). j. SASP omponents etete in our proteomis nlysis tht re ownregulte y mtorinhiitors, shmtor n ZFPL Mut. k. Venn igrms representing the overlp etween SASP ftors upregulte y mtor inhiitors, shmtor n ZFPL Mut. l. ARE Sore for SASP ftors: (left) ommonly upregulte y shmtor n mtor inhiitors; (right) ommonly ownregulte y shmtor, mtor inhiitors n ZFPL Mut. Plots enote ARE sore istriution for rnom omintions of n 7 mrnas respetively. Sttistil signifine ws lulte y using: (,e,j n i: Stuent s t-test) (k: hypergeometri test), (l: permuttion tests). P <.; P <.; P <.; n.s, non signifint. Error rs represent mens ± s.. For n h t re representtive of inepenent experiments. n represents numer of inepenent experiments. For unproesse originl sns of lots, see Supplementry Fig. 9. For rw t, see Supplementry Tle 7. Mmilln Pulishers Limite. All rights reserve
6 ARE Sore Rnom Set Enrihment sore FRIDMAN_SENESCENCE_UP NES =-.7 FDR q=. ZFPL Mut vs ER:RAS Enrihment sore SASP_COMPONENTS NES =-.97 FDR q=. ZFPL Mut vs ER:RAS ARE sore FC < FC < FC <. FC < FC <. < < - < -. < - < -. log (mut/ras) 9. Expression +OHT.... h h TIMP h h 7h ng/ml Dox. ng/ml Dox. TRE-ZFPL Mut ng/ml Dox. % Positive ells h h BrU BrU BrU h h 7h 7h nm Dox. nm Dox. nm Dox. e BrU BrU f +'OHT' Vetor' Vetor' p' % Positive ells % Positive ells Vetor Vetor p + OHT Vetor ZFPLMut Vetor' ZFPL Mut' 9%# %# 7%# 7#%# #%# %# SA-β-Gl Supplementry Figure Further hrteriztion of the role of ZFPL in the regultion of the SASP.. GSEAs of signture ssoite with senesen n (left) SASP omponents upregulte in OIS (right) in the gene expression profile (GEP) of IMR9 ER:RAS ells expressing ZFPL Mut versus ontrol IMR9 ER:RAS, oth OHT-trete. NES, normlize enrihment sore; FDR, flse isovery rte. GEPs were otine y performing trnsriptome sequening (RNA-Seq) of the forementione onitions in n= inepenent experiments. RNAs were ollete 7 ys fter inution with OHT.. Men ARE sore of genes own regulte (t the inite log of fol hnges) when ompring IMR9 ER:RAS ells expressing ZFPL Mut versus ontrol IMR9 ER:RAS, re shown in lue. The istriution of men ARE sores erive from simultions of rnom genes with equl smple size re shown in re.. This is n extension of Figure. The expression of TIMP ws monitore y qrt-pcr t the inite times. Dt re for representtive experiment of n= inepenent experiments. Bru inorportion ws mesure y IF t the inite times. Error rs represent the ± s.. from n= inepenent experiments. Stuent s T-test ws performe to ompre ZFPL Mut expressing ells (ng/ml oxyyline) to the ontrol senesent ells (, +OHT). P <..e-f. IMR9 ER:RAS expressing TRE-FLAG-ZFPL Mut (or empty ) n the reverse trnstivtor (rtta-m) were further infete with enoing the oing sequene of p. After ys of inution with OHT, the expression of ZFPL Mut ws inue with ng/ml oxyyline for 7h n (e) the % of BrU positive ells or (f) SA--Gl positive ells ws quntifie. Error rs represent the ± s.. from n= inepenent experiments. SA--Gl stinings re representtive of n= inepenent experiments. Stuent s T-test ws performe to ompre the inite onitions P<.;, non signifint. For rw t, see Supplementry Tle 7. Mmilln Pulishers Limite. All rights reserve
7 Herrnz et l Supplementry Figure 7 EMT inution CM IMR9 ER:RAS -/+ mtorinh. T7D EMT CM: + OHT Torin NVP-BEZ. E-herin Brightfiel IF:E-herin Intensity (.u.)..... Rpm. Torin NVP +OHT 9 SNAIL 9 ZEB SNAIL ZEB SNAIL ZEB SNAIL ZEB +$OHT%.. -OHT Vetor / Vetor Vetor / Vetor Vetor / p +OHT ZFPL Mut / Vetor ZFPL Mut / p E-herin TRE&EV$/$EV$ TRE&EV$/$EV$ TRE&EV$/$CDKNA&CDS$ TRE&ZFPL Mut$ /$EV$ TRE&ZFPL Mut$ /$CDKNA$CDS$ Fol Chnge... IF:$E&CADHERIN$. Supplementry Figure 7 The prrine protumorigeni effets of senesent ells re sensitive to mtor inhiition n ZFPL tivity.. mtor inhiition prevents the EMT inue y the CM of senesent ells. IMR9 ER:RAS ells were trete s inite in the sheme. CM ws ollete 7 ys fter OHT inution (mtor inhiitors were remove from the mei t y, see methos for etils) n T7D ells ulture in tht CM. h fter, E-Cherin expression ws monitore y IF. Fol hnges of normlise intensity vlues (top) n representtive pitures re shown (ottom). Dt re men ± s.. from n= inepenent experiments. Stuent s T-test ws performe to ompre trete ells to the ontrol. P <.. Sle r, mm.. Both inhiition of mtor signlling n expression of ZFPL Mut prevent the upregultion of EMT-speifi trnsription ftors y the CM of senesent ells. IMR9 ER:RAS ells were trete s inite in the sheme in (). CM ws ollete fter 7 ys. T7D ells were ulture in tht CM for the inite times n proesse for RNA extrtion. Snil n Ze levels were mesure y qrt-pcr. In the se of the CM of IMR9 ER:RAS trete with mtor inhiitors, t re men ± s.. from n= inepenent experiments. For the rest of the experiments t re from representtive experiment of n= inepenent experiments.. CM of oth proliferting n rreste ZFPL Mut expressing ells is unle to trigger omplete EMT. The inite ells were trete with OHT for 9 ys. Expression of TRE-ZFPL Mut ws tivte for the lst 7h. CM ws then ollete n T7D ells were ulture in tht CM for h. E-Cherin expression ws monitore y IF. Fol hnges of normlise intensity vlues (right) n representtive pitures re shown (left). Dt re men ± s.. from n= inepenent experiments.. Stuent s T-test ws performe to ompre ZFPL Mut ells to the ontrol (Vetor/ Vetor). P <.. Sle r, mm. For rw t, see Supplementry Tle Mmilln Pulishers Limite. All rights reserve
8 % % Positive positive ells mrna reltive expression (fol) IMR9 ER:RAS CM -/+ TRE-ZFPL Mut (-/+ CDKNA CDS)..... P=. Prrine senesene BrU IL IL P=. P=.9 IMR9 P=.9 -OHT +OHT Senesene Vetor / Vetor Vetor / Vetor Vetor / p ZFPL Mut / Vetor ZFPL Mut / p Herrnz et l Supplementry Figure Prrine senesene Co-ulture & IMR9 BrU IMR9 ER:RAS +/- shmtor + IMR9 & Conitione IMR9 ER:RAS: mei -OHT +OHT shmtor. shmtor. MAPKAPK& pebp T/T& EBP& GAPDH& e. tretment MCP RANTES CXCL CTACK NRsGV - 9 Nrs Nrs+ ells NRs g NRs GV NRs tretment p Cip Nrs+ ells p+ ells p+ ells Nrs NRs% p Cip p Ink p Ink f. mrna Reltive expression (fol)... P=. P=. P=. P=.. CD B CD KlrD Supplementry Figure mtor inhiition lunts the tumour suppressive effets of the SASP.. CMs of IMR9 ER:RAS expressing TRE- ZFPL Mut (or empty ) n p (or empty ) were ollete 7h fter inution of ZFPL Mut n 9 ys fter OHT ition. The effet CMs h on BrU inorportion n SASP inution in IMR9 wt ells ws evlute y IF. Grphs show t from n= inepenent experiments. IMR9 wt ells were o-ulture with the inite IMR9 ER:RAS ells with or without OHT. BrU inorportion ws monitore y IF. Dt re representtive of n= inepenent experiments.. This figure is relte to Fig.. Expression of the inite trnsripts ws quntifie y qrt-pcr., n= mie;, n= mie. P vlues re inlue.. Expression of MAPKAPK, EBP n phosphoryltion of EBP were nlyse y immunolot. Quntifition is presente in Fig. e. Nrs GV trnsposons were oelivere into mouse livers through hyroynmi injetion (Dy ). Mie were srifie ys lter n Nrs+ ells were ounte (n= mie per onition) Mie were trete with rrier or rugs from ys prior Nrs GV injetion. Representtive imges of the setions quntifie re shown (x). Sle r, mm. f. Quntifition of the expression of immune ell mrkers y qrt- PCR in livers of the inite mie ys fter NrsGV injetion., n= mie; rpmyin, n = mie. CD, T ell mrker; B, B ell mrker; CD, mrophge mrker; KlrD, NK ell mrker. P vlues re inlue. g. Nrs GV trnsposons were o-elivere into mie s expline efore. Mie were srifie ys lter. Mie were only trete with rrier or rpmyin ys post-injetion Nrs+ (left), p Cip + (entre) n p Ink + (right) ells on liver setions were ounte (n= mie per onition). Representtive imges of setions quntifie in the top pnel re shown in the ottom. Sle r, mm. Sttistil signifine ws lulte using Stuent s t-test, P <.; P <.; P <.; n.s, non signifint. Error rs represent mens ± s.. For unproesse originl sns of lots, see Supplementry Fig. 9. For rw t, see Supplementry Tle 7. Mmilln Pulishers Limite. All rights reserve
9 S U P P L E M E N TA R Y I N F O R M AT I O N Full sns of immunolots use in figures (I) Fig. / Fig. e GAPDH HSP7 S Fig./S EBP Fig. f Fig. Fig. i GAPDH HSP7 Supplementry Figure 9 Full sns of immunolots use in figures. 9 Mmilln Pulishers Limite. All rights reserve
10 S U P P L E M E N TA R Y I N F O R M AT I O N Full sns of immunolots use in figures (II) Fig. S Fig. Fig. S (IMR9) Fig. Se Fig. S (BJ) Supplementry Figure 9 ontinue Mmilln Pulishers Limite. All rights reserve
11 S U P P L E M E N TA R Y I N F O R M AT I O N Full sns of immunolots use in figures (III) Fig. S (HFFF) Fig. S pakts7 MK Fig. Fig. e Fig. Sg Supplementry Figure 9 ontinue Mmilln Pulishers Limite. All rights reserve
12 Supplementry Tles Legens Tle S Effet of the hemil ompouns in the levels of key SASP omponents. Tle S Seretome proteomis t for mtor inhiitors (normlise vlues). Tle S Phosphoproteomi t. Tle S Chemil ompouns use in the rug sreen. Tle S Primers, Tqmn proes n RNAi sequenes use in this stuy. Tle S Antioies use in this stuy. Tle S7 Rw t. Mmilln Pulishers Limite. All rights reserve
SUPPLEMENTARY INFORMATION
{ OI: 1.138/n31 Srifie n nlyze APs on week 1 s of iet 1 4 6 High-ft iet BrU High-ft iet BrU 4 High-ft iet BrU 6 High-ft iet BrU Lin - Lin - : C34 + : C9 + 1 1 3 1 4 1 5 C45 1 C34 1 1 1 1 3 1 4 1 5 S-1
More informationCos7 (3TP) (K): TGFβ1(h): (K)
IP#2: IP#1: Totl Lystes luiferse tivity (K): 6-4 - (K): luiferse tivity luiferse tivity (K): 2 1 RL-: - + + + + + Sm4-3F: + - + + + + MYC-Sm3: - - - - + + TβRI-HA(T204D): - - - + - + α-ha Luiferse Ativity
More informationSUPPLEMENTARY INFORMATION
doi: 1.138/nture862 humn hr. 21q MRPL39 murine Chr.16 Mrpl39 Dyrk1A Runx1 murine Chr. 17 ZNF295 Ets2 Znf295 murine Chr. 1 COL18A1 -/- lot: nti-dscr1 IgG hevy hin DSCR1 DSCR1 expression reltive to hevy
More informationAlimonti_Supplementary Figure 1. Pten +/- Pten + Pten. Pten hy. β-actin. Pten - wt hy/+ +/- wt hy/+ +/- Pten. Pten. Relative Protein level (% )
Alimonti_Supplementry Figure 1 hy 3 4 5 3 Neo 4 5 5 Proe 5 Proe hy/ hy/ /- - 3 6 Neo β-tin d Reltive Protein level (% ) 15 1 5 hy/ /- Reltive Gene Expr. (% ) 15 1 5 hy/ /- Supplementry Figure 1 Chrteriztion
More informationSUPPLEMENTARY INFORMATION
DOI: 1.138/n2977 Numer of ells per field 6 4 2 P =.1 Orthotopi eum Normlized ventrl photon flux 1E7 1E6 1E5 1E4 1E3 1E2 n=8 n=9 1 2 3 4 5 6 Dys Dy54 1.5E5 2.4E7 d Mie with lymph node metstsis (%) 1 8 6
More informationSUPPLEMENTARY INFORMATION
% ells with ili (mrke y A-Tu) Reltive Luiferse % ells with ili (mrke y Arl13) % ells with ili DOI: 1.138/n2259 A-Tuulin Hoehst % Cilite Non-ilite -Serum 9% 8% 7% 1 6% % 4% +Serum 1 3% 2% 1% % Serum: -
More informationSUPPLEMENTARY INFORMATION
DOI: 1.138/n358 TLR2 nd MyD88 expression in murine mmmry epithelil supopultions. CD24 min plus MRU Myo-epithelil Luminl progenitor (CD61 pos ) Mture luminl (CD61 neg ) CD49f CD61 Reltive expression Krt5
More informationSUPPLEMENTARY INFORMATION
DOI:.3/n95 Thymus Kiney (kd) TA T7 T TA T7 T Hert TA T7 T: +Dox Cylin B (kd) Thymus Kiney Hert TA T5 T TA T5 T TA T5 T: +Dox Cylin B Poneu S Poneu S CnB T7 CnB T Thymus (kd) + Liver Colon + + (kd) Thymus
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:.8/nture89 4 4 Ilr -/- Ilr -/- Ilr -/- Cspse- -/- As -/- Nlrp -/- Il8 -/- Ilr -/- Supplementl figure. Inresed severity of NASH in inflmmsome-defiient mie, ut not in Ilr-defiient
More informationsupplementary information
DOI:.38/n83 k Mouse Ch8 lous 8 9 Stop CHD8L 75 CHD8L Chromoomins Helise/ATPse omin DNA ining omin 5 kd NIH 3T3 MEF 93T HeL HCT UOS SOS.. CHD8L IB: CHD8 8 5 L S Reltive mrna mount 3... Reltive mrna mount.8.
More informationSUPPLEMENTARY INFORMATION
DOI: 1.13/n7 Reltive Pprg mrna 3 1 1 Time (weeks) Interspulr Inguinl Epididyml Reltive undne..1.5. - 5 5-51 51-1 1-7 7 - - 1 1-1 Lipid droplet size ( m ) 1-3 3 - - - 1 1-1 1-1 1-175 175-3 3-31 31-5 >5
More informationSupplemental Figures and Legends
Supplementl Figures n Legens Epigeneti trgeting of Hegehog trnsriptionl output through BET romoomin inhiition Yujie Tng 1,2, Shrreh Gholmin 2, Simone Shuert 1,2, Mine I. Willrson 3, Alex Lee 4, Prtiti
More informationa3 Chains of type V collagen regulate breast tumour growth via glypican-1
Reeive 5 Aug 16 Aepte De 16 Pulishe 19 Jn 17 3 Chins of type V ollgen regulte rest tumour growth vi glypin-1 Guorui Hung 1, Goxing Ge 1,w, Vlerio Izzi & Dniel S. Greenspn 1 DOI: 1.138/nomms1351 OPEN Periellulr
More informationSupplementary Figure S1_Cottini
Supplementry Figure S1_Cottini γ-h2a.x Krp OCIMy5 KMS11 Krps62 RPMI8226 INA6-1 µm Cleve C3 γ-h2a.x DAPI Merge OCIMy5 H929 JJN3 UTMC2 KMS11 KMS12PE KMS18 KMS2 RPMI8226 INA6 U266 KMS34 Krps62 1 2 3 4 5 6
More informationnestin ironetin p75 s1 CNS SKPs Dermo-1 +ve SKPs CNS H2O SCGs Skin Di. SKPs TH SHOX2 GAPDH NCAM D H Figure S1, Immunoytohemil nlysis o SKP spheres ulture rom neontl mouse (nestin, ironetin, S-1) or rt
More informationSUPPLEMENTARY INFORMATION
oi:1.138/nture1138 Supplementl Figure 1 Inflmmtory Monoytes Host ells CCR2 CCL2 Disseminting Tumor Cells Metstsis Assoite Mrophges VEGF Extrvstion & Metstti Seeing Supplementl Figure 1 The t from this
More informationSUPPLEMENTARY INFORMATION
doi:.8/nture98 : hr NEMO :5 hr IKK IKK NF-κB p65 p5 p65/-rel NF-κB p65 p5 p65/-rel Cytoplsm Cytoplsm p65/p5 Nuleus Nuleus NEMO IKK IKK d : hr > : hr p65/-rel NF- p65 p5 Cytoplsm Cytoplsm p65/p5 p65/-rel
More informationSUPPLEMENTARY INFORMATION
Prentl doi:.8/nture57 Figure S HPMECs LM Cells Cell lines VEGF (ng/ml) Prentl 7. +/-. LM 7. +/-.99 LM 7. +/-.99 Fold COX induction 5 VEGF: - + + + Bevcizum: - - 5 (µg/ml) Reltive MMP LM mock COX MMP LM+
More information(% of adherent cells) *** PBL firm adhesion. Frequency (% ) 4 1 L 2 CXCR3 DP-2
Chemotxis (% of dded ells) PBL totl dhesion (N ells/mm 2 /1.1 6 PBL) Frequeny (% ) PBL firm dhesion Supplementry Figure 1 4 4 3 3 2 2 1.1-4 1-3 1.1.2. 1 1 8 6 4 2 Adiponetin ( g/ml) - + Adiponetin ( g/ml)
More informationSupplementary Figure S1
Supplementry Figure S Connexin4 TroponinI Merge Plsm memrne Met Intrcellulr Met Supplementry Figure S H9c rt crdiomyolsts cell line. () Immunofluorescence of crdic mrkers: Connexin4 (green) nd TroponinI
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION CD169 + MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES Ptrii Brrl, Polo Polzell, Andres Brukuer, Nio vn Rooijen, Gurdyl S.
More informationSUPPLEMENTARY INFORMATION
DOI:.38/nc393 Nnog DAPI Nnog/DAPI c-jun DAPI c-jun/dapi c e Reltive expression to Gpdh mescs ( Feeder free) mescs ( Feeder) MEFs.5 MEFs ipscs ESCs..5 p=.24 p=.483 p=.22. JunB JunD Fos Fr Fr2 ATF2 ATF3
More informationSupplementary Information
Supplementry Informtion Non-nonil prevents skeletl ging n inflmmtion y inhiiting NF-κB Bo Yu, Ji Chng, Yunsong Liu, Jiong Li, Kreen Kevork, Khli Al Hezimi, Dn T. Grves, No-Hee Prk, Cun-Yu Wng Supplementry
More informationTargeting BIG3 PHB2 interaction to overcome tamoxifen resistance in breast cancer cells
Reeive Fe 13 Aepte 15 Aug 13 Pulishe Sep 13 DOI: 1.13/nomms33 OPEN Trgeting intertion to overome tmoxifen resistne in rest ner ells Tetsuro Yoshimru 1, Msto Komtsu 1, Tisuke Mtsuo 1, Yi-An Chen, Yoihi
More informationSupplementary Figure S1
Supplementry Figure S1 - UTR m - 3HA - 2-1 hgh - 1 Uiquitin *! *! lk distl promoter m K3R/ K121R-3HA UTR hgh founder lines - HA - - founder lines TG- E1 L A2 B1 F9 G6 H4 H6 B C D2 G1 H3 J2 L - 7 IP: lk
More informationSUPPLEMENTARY INFORMATION
doi:1.138/nture1794 BR EPFs BRI1? ERECTA TMM BSKs YDA PP2A BSU1 BIN2 pbzr1/2 BZR1/2 MKK4/5/7/9 MPK3/6 SPCH Cell growth Stomtl production Supplementry Figure 1. The model of BR nd stomtl signling pthwys.
More informationOther Uses for Cluster Sampling
Other Uses for Cluster Smpling Mesure hnges in the level of n ttriute Hypothesis testing versus intervl estimtion Type I n 2 errors Power of the test Mesuring ttriute t sme time in ifferent sites Exmple:
More information% of Nestin-EGFP (+) cells
8 7 6 3 Nestin CD % of Nestin-EGFP (+) ells 8 6 Nestin-EGFP Marge Nestin-EGFP Marge Expression levels of Expression levels of Tumorigeniity y stemness markers ifferentiation markers limiting ilution assay
More informationRestoration of p53 function leads to tumour regression in vivo. p53 locus. Targeting vector DTA LSL. Targeted allele
Vol 445 8 Ferury 27 oi:1.138/nture5541 Restortion of funtion les to tumour regression in vivo Anre Ventur 1, Dvi G. Kirsh 1,2, Mrgret E. MLughlin 1, Dvi A. Tuveson 1, Jn Grimm 3, Lur Lintult 1, Jmie Newmn
More informationSupplementary Figure 1. Scheme of unilateral pyramidotomy used for detecting compensatory sprouting of intact CST axons.
() BDA 2 weeks fter Py () AAVs Cre or GFP t P1 BDA 2 weeks fter Py CSMN CST () Py t P7 or 2 months () Py t 2 months Supplementry Figure 1. Sheme of unilterl pyrmidotomy used for deteting ompenstory sprouting
More informationTitle of Experiment: Author, Institute and address:
Title of Experiment: Trsfetion of murine mrophge RAW264.7 ells with METAFECTENE PRO. Author, Institute n ress: Ptrizi Pellegtti n Frneso Di Virgilio. Deprtment of Experimentl n Dignosti Meiine, Setion
More informationN6-methyladenosine (m6a) is the most prevalent messenger
https://oi.org/8/s556-8-7- m 6 A mrna methyltion regultes tivity to promote the prolifertion n tumorigeniity of enometril ner Jun Liu,,, Mrk A. Ekert,, Bryn T. Hr,,, Song-Mei Liu,, Zhike Lu,, Kngkng Yu,,5,
More informationLHb VTA. VTA-projecting RMTg-projecting overlay. Supplemental Figure 2. Retrograde labeling of LHb neurons. a. VTA-projecting LHb
SUPPLEMENTARY INFORMATION Supplementl Figure 1 doi:10.1038/nture09742 Lterl 1.0 mm from midline mpfc BNST mpfc BNST Lterl 2.1 mm from midline LHA LHA Lterl 2.7 mm from midline SUPPLEMENTAL INFORMATION
More information100 μm. Axon growth cones. Tubulin (red) + scr (green)
Supplementary Figures mirorna-9 regulates axon extension an ranhing y targeting Map1 in mouse ortial neurons Dajas-Bailaor, F., Bonev, B., Garez, P., Stanley, P., Guillemot, F., Papalopulu, N. a 2 μm 1
More informationLesions of prefrontal cortex reduce attentional modulation of neuronal responses. and synchrony in V4
Lesions of prefrontl ortex reue ttentionl moultion of neuronl responses n synhrony in V4 Georgi G. Gregoriou,, Anrew F. Rossi, 3 Leslie G Ungerleier, 4 Roert Desimone 5 Deprtment of Bsi Sienes, Fulty of
More informationChow KD CR HFD. Fed Fast Refed
Supplementry Figure 1 Control d/d Chow KD CR Fed Fst Refed Supplementry Figure 1: Liver expression in diet nd disese models. () expression in the livers of ontrol nd d/d mie. () expression in the livers
More informationAcute and gradual increases in BDNF concentration elicit distinct signaling and functions in neurons
nd grdul increses in BDNF concentrtion elicit distinct signling nd functions in neurons Yunyun Ji,, Yun Lu, Feng Yng, Wnhu Shen, Tin Tze-Tsng Tng,, Linyin Feng, Shumin Dun, nd Bi Lu,.. - Grdul (normlized
More informationEFFECT OF SOYBEAN CYST NEMATODE ON GROWTH OF DRY BEAN. Research Report to Northarvest Bean Growers, January 19, 2009
EFFECT OF SOYBEAN CYST NEMATODE ON GROWTH OF DRY BEAN Reserh Report to Northrvest Ben Growers, Jnury 19, 29 Berlin D. Nelson, Susilo Poromrto, n Ruell Goswmi, Dept. Plnt Pthology, NDSU Ojetive: Determine
More informationTbp. Per Relative mrna levels Circadian Time. Liver weight/ body weight (%) n.s. Pernull
Liver weight/ ody weight (%) Dy Body weight (g) Reltive mrna levels Reltive mrna levels Reltive mrna levels Reltive mrna levels Dy Per1 Per2 Per3 Tp 8 2 8 2. 6 2 8 12162 Cirdin Time 3 2 1 2 1 1 8 12162
More informationTNF-α (pg/ml) IL-6 (ng/ml)
Xio, et l., Supplementry Figure 1 IL-6 (ng/ml) TNF-α (pg/ml) 16 12 8 4 1,4 1,2 1, 8 6 4 2 med Cl / Pm3CSK4 zymosn curdln Poly (I:C) LPS flgelin MALP-2 imiquimod R848 CpG TNF-α (pg/ml) IL-6 (ng/ml) 2 1.6
More informationSupplementary figure 1
Supplementry figure 1 Dy 8 post LCMV infection Vsculr Assoc. Prenchym Dy 3 post LCMV infection 1 5 6.7.29 1 4 1 3 1 2 88.9 4.16 1 2 1 3 1 4 1 5 1 5 1.59 5.97 1 4 1 3 1 2 21.4 71 1 2 1 3 1 4 1 5 1 5.59.22
More informationSUPPLEMENTARY INFORMATION
SUPPEMENTARY INFORMATION DOI: 1.138/ncb956 Norml CIS Invsive crcinom 4 months months b Bldder #1 Bldder # Bldder #3 6 months (Invsive crcinom) Supplementry Figure 1 Mouse model of bldder cncer. () Schemtic
More informationSupplementary information for: Low bone mass and changes in the osteocyte network in mice lacking autophagy in the osteoblast lineage
Supplementry informtion for: Low one mss nd chnges in the osteocyte network in mice lcking utophgy in the osteolst linege Mrilin Piemontese, Meld Onl, Jinhu Xiong, Li Hn, Jeff D. Thostenson, Mri Almeid,
More informationAbortion frequency (%) Ovary position on ear Ovary volume (mm 3 )
ortion frequeny (%) 5 1 Ovry position on er 3 1 WW WD pex Bse Ovry volume (mm 3 ) Figure S1. Ovry volume (thik lines) n ortion frequeny (thin lines) s funtion of position long the er, 15 ys fter silk emergene
More informationRegulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling
Reeive Mr Aepte Aug Publishe 8 Sep DOI:.8/nomms97 Regultion of NKT ell-meite immune responses to tumours n liver inflmmtion by mitohonril PGAM-Drp signlling Young Jun Kng, Bo-Rm Bng, Kyung Ho Hn, Lixin
More informationb-sitosterol activates Fas signaling in human breast cancer cells
ARTICLE IN PRESS Phytomeiine 14 (2007) 747 754 www.elsevier.e/phyme -Sitosterol tivtes Fs signling in humn rest ner ells A.B. Aw,, M. Chinnm, C.S. Fink, P.G. Brfor Deprtment of Exerise n Nutrition Sienes
More informationSUPPLEMENTARY INFORMATION
oi:1.138/nture1134 CS+ CS- MCH 3 OCT OCT 3 MCH CS- CS+ OCT MCH 3 MCH OCT 3 OCT vs MCH OCT vs MCH ppetitive memory (PI) A 1-1 Unpire onitioning DDC-GAL4/UAS-Trp UAS-Trp/+ -2 MCH OCT OCT MCH sugr OCT MCH
More informationRole of the EGF receptor in PPARγ-mediated sodium and water transport in human proximal tubule cells
Dietologi (213) 56:1174 1182 DOI 1.17/s125-13-2835-y ARTICLE Role of the EGF reeptor in PPARγ-meite soium n wter trnsport in humn proximl tuule ells S. S & J. Zhng & R. Yong & D. Yghoin & M. G. Wong &
More informationSupplementary Figure 1
Roles of endoplsmic reticulum stress-medited poptosis in -polrized mcrophges during mycocteril infections Supplementry informtion Yun-Ji Lim, Min-Hee Yi, Ji-Ae Choi, Jung-hwn Lee, Ji-Ye Hn, Sung-Hee Jo,
More informationDOI: 10.1038/nc2331 PCre;Ros26R 12 h induction 48 h induction Vegfr3 i EC c d ib4 24 h induction VEGFR3 e Fold chnge 1.0 0.5 P < 0.05 Vegfr3 i EC Vegfr3 Figure S1 Cre ctivtion leds to genetic deletion
More informationc-abl inhibition mitigates diet-induced obesity through improving insulin sensitivity of subcutaneous fat in mice
Dietologi (7) :9 9 DOI.7/s--- ARTICLE -Al inhiition mitigtes iet-inue oesity through improving insulin sensitivity of suutneous ft in mie Rong Wu, & Jin-gung Sun, & Ji-qiu Wng & Binhu Li & Qingsong Liu
More informationSupplementary Figure S1
Supplementry Figure S1 d MAP2 GFAP e MAP2 GFAP GFAP c f Clindin GFAP Supplementry Figure S1. Neuronl deth nd ltered strocytes in the rin of n ffected child. Neuron specific MAP2 ntiody stining in the hippocmpus
More informationA liver HIF-2α/IRS2 pathway sensitizes hepatic insulin signaling and is modulated by VEGF inhibition
A liver HIF-2α/IRS2 pthwy sensitizes hepti insulin signling n is moulte y VEGF inhiition Kevin Wei1,1, Stephnie M. Pieewiz1,1, Lis M. MGinnis1,1, Cullen M. Tniguhi2, Stnley J. Wiegn3, Keith Anerson3, Crol
More informationSUPPLEMENTARY INFORMATION
2 weeks high holesterol diet 2 weeks high holesterol diet 2 weeks high holesterol diet 2 μm Mrophges Crystls Hoehst μm Mrophges Crystls Hoehst Hoehst Crystls Mrophges 2 μm 2 μm Supplementry Fig. 1: Erly
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:1.18/nture129 ontrol-dna -DNA CD49 Blood Lung e.98 +/-.9.71 +/-.2.29+/-.1 2.9 +/-.6 Bsophils (x1 )/ml 4 Bsophils ( x1 ) d f 45. 22.5 15 75 ontrol-dna ontrol-dna -DNA -DNA
More informationSUPPLEMENTARY INFORMATION
doi:1.138/nture17 Men tumour dimeter (mm) 2 Rg2-/- 2 1 2 2 1 Control IgG!-CD8!-CD4 1 2 3 1 2 3 c Men tumour dimeter (mm) 2 2 1 d Ifnr1-/- Rg2-/- 2 2 1 Ifngr1-/- d42m1!ic 1 2 3 Dys post trnsplnt 1 2 3 Supplementry
More informationSUPPLEMENTARY INFORMATION
. Norml Physiologicl Conditions. SIRT1 Loss-of-Function S1. Model for the role of SIRT1 in the regultion of memory nd plsticity. () Our findings suggest tht SIRT1 normlly functions in coopertion with YY1,
More informationEffects of Enzyme Inducers in Therapeutic Efficacy of Rosiglitazone: An Antidiabetic Drug in Albino Rats
Asin J. Exp. Si., Vol. 21, No. 2, 2007, 00-00 Effets of Enzyme Inuers in Therpeuti Effiy of Rosiglitzone: An Antiieti Drug in Alino Rts Ann Chursi,#* P.K. Krr** A. S. Mnn* & M.D. Khry* * Deprtment of Phrmeutil
More informationOriginal article HIV-1 Tat protein impairs adipogenesis and induces the expression and secretion of proinflammatory cytokines in human SGBS adipocytes
Antivirl Therpy 2012; 17:529 540 (oi: 10.3851/IMP2021) Originl rtile HIV-1 Tt protein impirs ipogenesis n inues the expression n seretion of proinflmmtory ytokines in humn SGBS ipoytes Juliet Díz-Delfín
More informationSUPPLEMENTARY INFORMATION
doi: 10.1038/nture07679 Emryonic Stem (ES) cell Hemngiolst Flk1 + Blst Colony 3 to 3.5 Dys 3-4 Dys ES differentition Sort of Flk1 + cells Supplementry Figure 1. Chrcteristion of lst colony development.
More informationCoatomer LPAAT-γ Merge
DI: 1.138/n2273 Cotomr LPAAT-γ Mrg Tuuls (%) 1 5 < 5 nm 5~1nm 1~2nm 2~5nm >5nm 5~1nm < 5 nm 1~2nm 2~5nm >5nm 2 min 5 min 1 min 3 min < 5 nm 5~1nm 1~2nm 2~5nm >5nm < 5 nm 5~1nm 1~2nm 2~5nm >5nm Figur S1
More informationLean, but not obese, fat is enriched for a unique population of regulatory T cells that affect metabolic parameters
Len, ut not oese, ft is enrihe for unique popultion of regultory T ells tht ffet metoli prmeters Mrkus Feuerer,5, Lur Herrero 2,5, Dniel Cipollett,4,5, Afi Nz 2, Jmie Wong,5, Ali Nyer 2, Jongsoon Lee 2,
More informationRapamycin toxicity in MIN6 cells and rat and human islets is mediated by the inhibition of mtor complex 2 (mtorc2)
Dietologi (212) 55:1355 1365 DOI 1.17/s125-12-2475-7 ARTICLE myin toxiity in MIN6 ells nd rt nd humn islets is medited y the inhiition of mtor omplex 2 (mtorc2) A. D. Brlow & J. Xie & C. E. Moore & S.
More informationEFFECTS OF AN ACUTE ENTERIC DISEASE CHALLENGE ON IGF-1 AND IGFBP-3 GENE EXPRESSION IN PORCINE SKELETAL MUSCLE
Swine Dy 22 Contents EFFECTS OF AN ACUTE ENTERIC DISEASE CHALLENGE ON IGF-1 AND IGFBP-3 GENE EXPRESSION IN PORCINE SKELETAL MUSCLE B. J. Johnson, J. P. Kyser, J. D. Dunn, A. T. Wyln, S. S. Dritz 1, J.
More informationSUPPLEMENTARY INFORMATION
doi:.38/nture277 d 25 25 2 Time from sound onset (ms) 25 25 2 Time from sound onset (ms) Firing rte (spikes/s) Firing rte (spikes/s).8.6..2 e f g h.8.6..2 Frtion of neurons Frtion of neurons N = 53 2 2
More informationLETTER. A restricted cell population propagates glioblastoma growth after chemotherapy
oi:1.138/nture11287 A restrite ell popultion propgtes glioblstom growth fter hemotherpy Jin Chen 1, Ynjio Li 1, Tzong-Shiue Yu 1,2 {, Renée M. MKy 1, Dennis K. Burns 3, Steven G. Kernie 1,2 { & Luis F.
More informationCopy Number ID2 MYCN ID2 MYCN. Copy Number MYCN DDX1 ID2 KIDINS220 MBOAT2 ID2
Copy Numer Copy Numer Copy Numer Copy Numer DIPG38 DIPG49 ID2 MYCN ID2 MYCN c DIPG01 d DIPG29 ID2 MYCN ID2 MYCN e STNG2 f MYCN DIPG01 Chr. 2 DIPG29 Chr. 1 MYCN DDX1 Chr. 2 ID2 KIDINS220 MBOAT2 ID2 Supplementry
More informationInhibitory effect of p38 mitogen-activated protein kinase inhibitors on cytokine release from human macrophages
British Journl of Phrmology (26) 149, 393 44 & 26 Nture Pulishing Group All rights reserved 7 1188/6 $3. www.rjphrmol.org RESEARCH PAPER Inhiitory effet of p38 mitogen-tivted protein kinse inhiitors on
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi: 1.138/nnno.211.41 Sili nd titnium dioxide nnoprtiles use pregnny omplitions in mie Kohei Ymshit, Ysuo Yoshiok, Kzum Higshisk, Kzuy Mimur, Yuki Morishit, Mstoshi Nozki, Tokuyuki
More informationInhibition of Dexamethasone-induced Fatty Liver Development by Reducing mir-17-5p Levels
originl rtile Inhiition of Dexmethsone-inue Ftty Liver Development y Reuing -5p Levels Willim W Du,, Fengqiong Liu 3, Sze Wn Shn,, Xini Ciny M,, Shn Gupt,, Tinru Jin 4, Dvi Spner, Sergey N Krylov 5, You
More informationLETTER. Oxidative stress induces angiogenesis by activating TLR2 with novel endogenous ligands
oi:.8/nture9 Oxitive stress inues ngiogenesis y tivting TLR with novel enogenous ligns Xioxi Z. West, *, Nikoly L. Mlinin *, Alon A. Merkulov, Mir Tishenko, Bethny A. Kerr, Ernest C. Boren, Eugene A. Porez,
More informationThe GCN5-CITED2-PKA signalling module controls hepatic glucose metabolism through a camp-induced substrate switch
Reeived 6 Apr 216 Aepted 8 Sep 216 Pulished 22 Nov 216 DOI: 1.138/nomms13147 OPEN The GCN5-CITED2-PKA signlling module ontrols hepti gluose metolism through AMP-indued sustrte swith Mshito Ski 1, Tomoko
More informationTranscription factor Ets-1 links glucotoxicity to pancreatic beta cell dysfunction through inhibiting PDX-1 expression in rodent models
Dietologi () 9: DOI.7/s ARTICLE Trnsription ftor Ets links gluotoxiity to pnreti et ell ysfuntion through inhiiting PDX expression in roent moels Fng Chen & Min Sh & Ynyng Wng & Tijun Wu & Wei Shn & Ji
More informationSUPPLEMENTARY INFORMATION
DOI: 1.138/nc286 Figure S1 e f Medium DMSO AktVIII PP242 Rp S6K1-I Gr1 + + + + + + Strvtion + + + + + IB: Akt-pT38 IB: Akt K-pT389 K IB: Rptor Gr1 shs6k1-a shs6k1-b shs6k1-c shrictor shrptor Gr1 c IB:
More informationER-α36 mediates cisplatin resistance in breast cancer cells through EGFR/HER-2/ ERK signaling pathway
Zhu et l. Journl of Experimentl & Clinil Cner Reserh (218) 37:123 https://oi.org/1.1186/s134618798z RESEARCH Open Aess ERα36 meites ispltin resistne in rest ner ells through /HER2/ signling pthwy Linlin
More informationYAP transcriptionally regulates COX-2 expression and GCCSysm-4 (G-4), a dual YAP/COX-2 inhibitor, overcomes drug resistance in colorectal cancer
Li et l. Journl of Experimentl & Clinil Cner Reserh (7) 36:44 DOI.86/s346-7-6-3 RESEARCH Open Aess trnsriptionlly regultes expression nd GCCSysm-4 (G-4), dul / inhiitor, overomes drug resistne in oloretl
More informationProvider How To. Software Process Service Results
Softwre Proess Servie Results Provier How To Copyright Glenwoo Systems LLC 2010. The informtion herein remins the property of Glenwoo Systems LLC. This informtion my not e reprinte or uplite, n is governe
More informationTNF-a Downregulates Filaggrin and Loricrin through c-jun N-terminal Kinase: Role for TNF-a Antagonists to Improve Skin Barrier
ORIGINAL ARTICLE TNF- Downregultes Filggrin nd Loririn through -Jun N-terminl Kinse: Role for TNF- Antgonists to Improve Skin Brrier Byung Eui Kim, Mihel D. Howell,, Emm Guttmn,, Ptrii M. Gilleudeu, Irm
More informationSonic Hedgehog promotes proliferation of Notch-dependent monociliated choroid plexus tumour cells
Soni Hedgehog promotes prolifertion of Noth-dependent monoilited horoid plexus tumour ells Li Li, Ktie B. Grusm,2, Jun Wng 3, Melody P. Lun 4,5, Jsmin Ohli 6, Hrt G. W. Lidov 4, Moni L. Clihio 4, Erling
More informationMacrophage mtorc1 disruption reduces inflammation and insulin resistance in obese mice
Dietologi (1) 7:393 DOI 1.17/s-1-33- ARTICLE Mrophge mtorc1 isruption reues inflmmtion n insulin resistne in oese mie Hongfeng Jing & Mrit Westerterp & Chunjiong Wng & Yi Zhu & Ding Ai Reeive: 1 April
More informationFates-shifted is an F box protein that targets Bicoid for degradation and regulates developmental fate determination in Drosophila embryos
ARTICLES Ftes-shifted is n F ox protein tht trgets Bioid for degrdtion nd regultes developmentl fte determintion in Drosophil emryos Juno Liu 1 nd Jun M 1,2,3 Bioid (Bd) is morphogeneti protein tht instruts
More informationDocosapentaenoic Acid (22:5n-3) Downregulates mrna Expression of Pro-inflammatory Factors in LPS-activated Murine Macrophage Like RAW264.
Journl of Oleo Siene Copyright 217 y Jpn Oil Chemists Soiety oi : 1.565/jos.ess17111 Doospentenoi Ai (22:5n-3) Downregultes mrna Expression of Pro-inflmmtory Ftors in LPS-tivte Murine Mrophge Like RAW264.7
More informationRoquin binds inducible costimulator mrna and effectors of mrna decay to induce micrornaindependent post-transcriptional repression
ins inuile ostimultor mrna n effetors of mrna ey to inue mirornainepenent post-trnsriptionl repression Elke Glsmher 1, Ki P Hoefig 1,3, Kthrin U Vogel 1,3, Niol Rth 1, Lirui Du 1, Christine Wolf 1, Eliseth
More informationShear behaviour of regular and irregular rock joints under cyclic conditions
Pper No. 69 ISMS 2016 Sher ehviour of regulr n irregulr rok joints uner yli onitions S. M. Mhi Niktr, *, K. Seshgiri Ro, Amit Kumr Shrivstv Deprtment of Civil Engineering, Inin Institute of Tehnology Delhi,
More information* * * * * liver kidney ileum. Supplementary Fig.S1
Supplementry Fig.S1 liver kidney ileum Fig.S1. Orlly delivered Fexrmine is intestinlly-restricted Mice received vehicle or Fexrmine (100mg/kg) vi per os (PO) or intrperitonel (IP) injection for 5 dys (n=3/group).
More informationAMPK maintains energy homeostasis and survival in cancer cells via. regulating p38/pgc-1α-mediated mitochondrial biogenesis
SUPPLEMENTARY INFORMATION AMPK mintins energy homeostsis nd survivl in cncer cells vi regulting p38/pgc-1α-medited mitochondril iogenesis Blkrishn Chue 1, Prmnnd Mlvi 1, Shivendr Vikrm Singh 1, Noshd Mohmmd
More informationPDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis
Supplementry Informtion PDGF-BB secreted y preosteoclsts induces ngiogenesis during coupling with osteogenesis Hui Xie, Zhung Cui, Long Wng, Zhuying Xi, Yin Hu, Lingling Xin, Chngjun Li, Ling Xie, Jnet
More informationMicrotubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome
Supplementry Informtion Microtuule-driven sptil rrngement of mitochondri promotes ctivtion of the NLRP3 inflmmsome Tkum Misw 1,2, Michihiro Tkhm 1,2, Ttsuy Kozki 1,2, Hnn Lee 1,2, Jin Zou 1,2, Ttsuy Sitoh
More informationDGCR8 is essential for microrna biogenesis and silencing of embryonic stem cell self-renewal
7 Nture Pulishing Group http://www.nture.om/nturegenetis DGCR is essentil for mirorna iogenesis n silening of emryoni stem ell self-renewl Yngming Wng, Rostislv Mevi, Collin Melton, Ruolf Jenish & Roert
More informationFarnesoid X receptor inhibits glucagon-like peptide-1 production by enteroendocrine L cells
Reeive 27 Mr 215 Aepte 25 My 215 Pulishe 2 Jul 215 DOI: 1.138/nomms8629 Frnesoi X reeptor inhiits glugon-like peptie-1 proution y enteroenorine L ells Mohme-Smi Trelsi 1,2,3,4, Mehi Doui 1,2,3,4, Jnne
More informationPTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia
A RT I C L E S PTP1B ontrols non-mitohonril oxygen onsumption y regulting to promote tumour survivl uring hypoxi Roert S. Bnh 1,2,3, Cterin Iorio 2,11, Rihr Mrotte 2,11, Yng Xu 1,2,3,11, Dn Cojori 1,2,
More informationSUPPLEMENTARY INFORMATION
~ 5 % ltion > 99 % ltion 25 2 15 5 Gly yemi (mmol/l) 3 7 15 3 Time fter -ell ltion (dys) Survivl (%) & ~ 5 % ltion 75 > 99% ltion 5 25 25 5 (dys) 7.4 d 1.25 lood ph 73 7.3 7.2 7.1 7. 7d ody weight h nge
More informationARTICLES. Mediators of vascular remodelling co-opted for sequential steps in lung metastasis
Vol 1 April 7 doi:1.13/nture57 ARTICLES Meditors of vsulr remodelling o-opted for sequentil steps in lung metstsis Gorv P. Gupt 1, Don X. Nguyen 1, Anne C. Ching 1,, Pul D. Bos 1, Juliet Y. Kim 1, Cristin
More informationThe microrna mir-31 inhibits CD8 + T cell function in chronic viral infection
A rt i l e s The mirorna mir-3 inhiits CD8 + T ell funtion in hroni virl infetion Howell F Moffett, Adm N R Crtwright, Hye-Jung Kim, Jernej Gode, Json Pyrdol, Trmo Äijö 3, Gustvo J Mrtinez,6, Anjn Ro,
More informationThe Hippo/YAP pathway interacts with EGFR signaling and HPV oncoproteins to regulate cervical cancer progression
Reserh Artile The Hippo/ pthwy interts with EGFR signling nd HPV onoproteins to regulte ervil ner progression Chuno He 1,, Dgn Mo 1,3, Guohu Hu 1,, Xingmin Lv 1, Xingheng Chen, Peter C Angeletti 5, Jixin
More informationInterplay of LRRK2 with chaperone-mediated autophagy
Interply of with hperone-medited utophgy Smnth J Orenstein,, Sheng-Hn Kuo,, Inmuld Tsset,,, Espernz Aris,, Hiroshi Kog,, Irene Fernndez-Crs, Etty Cortes,5, Lwrene S Honig,5, Willim Duer 6, Antonell Consiglio,7,
More informationPellino3 targets the IRF7 pathway and facilitates autoregulation of TLR3- and viral-induced expression of type I interferons
Pellino3 trgets the pthwy n filittes utoregultion of TLR3- n virl-inue expression of type I interferons Jku Sienienko 1,, Ruihri Jkson 1,, Mrk Mellett 1,, Nezir Delgi 1, Shuo Yng 1, Bingwei Wng 1, Lis
More informationRNAi Targeting CXCR4 Inhibits Tumor Growth Through Inducing Cell Cycle Arrest and Apoptosis
originl rtile RNAi Trgeting CXCR4 Inhiits Tumor Growth Through Induing Cell Cyle Arrest nd Apoptosis To Yu 1,2, Yingying Wu 2, Yi Hung 1,2, Chorn Yn 1, Ying Liu 1, Zongsheng Wng 3, Xioyi Wng 1, Yuming
More information*** *** *** *** T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. T-cells T-ALL. Relative ATP content. Relative ATP content RLU RLU
RLU Events 1 1 1 Luciferin (μm) T-cells T-ALL 1 1 Time (min) T-cells T-ALL 1 1 1 1 DCF-DA Reltive ATP content....1.1.. T-cells T-ALL RLU 1 1 T-cells T-ALL Luciferin (μm) 1 1 Time (min) c d Control e DCFH-DA
More informationParathyroid hormone related peptide is a naturally occurring, protein kinase A dependent angiogenesis inhibitor
Prthyroi hormone relte peptie is nturlly ourring, protein kinse A epenent ngiogenesis inhiitor MANJIRI M. BAKRE 1, YUHONG ZHU 1, HONG YIN 1, DOUG W. BURTON 2, ROBERT TERKELTAUB 2, LEONARD J. DEFTOS 2 &
More information