Dendritic Cell - T Cell Immune Network

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1 w w w. i m g e n e x. c o m BRIDGING INNATE & ADAPTIVE IMMUNITY Dendritic Cell - T Cell Immune Network Signaling Pathways IMGENEX provides the most extensive portfolio for in depth analysis of the Dendritic Cell - T Cell network including: IL-10 IL-35 TGF T H 17 IL-17A IL-17E IL-17F IL-21 Cell Analysis - Flow Cytometry Signaling Pathway Molecules Cytokines and Inflammatory Mediators Stable Reporter Cell Lines TLR Agonists and Antagonists Pathogen Treg IL-2 TGF IL-6 IL-21 IL-23 TGF HLA-DR PerCP-Cy5.5 hlmax LinFITC pdc Engulfment CD4 + T cell TCR MHC II TLRs NF- B CD80/86 CD28 IL-12 IFN IgG1 AF647 CD123 AF647 pdc/tlr9 Proinflammatory Cytokines (IL-1, IL-12, IFN, TNF ) DC IL-2 IL-4 T H 1 IL-4 IL-5 IL-13 IL-25 T H 2 IFN IL-2 TNF IgG1 PE TLR9 PE Human PBMCs stained with hlmax Human Lineage Marker/HLA-DR Antibody Mix (IMG-6628K); CD123, Human Plasmacytoid DC Identification Kit (IMG-6629K); TLR9 PE Human Plasmacytoid DC/TLR9 Investigation Kit (IMG-6630K) Dendritic Cells hlmax Human Lineage Marker/HLA-DR Antibody Mix Plasmacytoid Dendritic Cell (pdc) Identification Kit Plasmacytoid Dendritic Cell (pdc)/tlr9 Investigation Kit pdc/idc CD14 CD207 CD40 BDCA-2/CD303 CD80 DC-SIGN/CD209 CD83 CD11b CD86 CD11c CD123 T Cells, T H 17 & Treg CD3 GITRL CD4 IL-17 CD8 IL-23 Receptor CD25 IL-33 CD40L inkt CD127/IL7R FOXP3 Folate Receptor 4 FOXP3 2 GARP GPR83 GITR ROR /ROR (t) TLR Ligands/Agonists NF- B Pathways TM ActivELISA Kits for Cytokines, Chemokines + Inflammatory Mediators TLR & TLR/NF- B SEAPorter Reporter Cell Lines TM Please visit us at Toll-free (888) Fax (858) info@imgenex.com w w w. i m g e n e x. c o m

2 The MACSQuant Analyzer A new milestone in flow cytometry Excite and Inspire. Compact Fits into a standard laminar flow hood Capable 7 fluorescence channels and absolute cell counting on up to 96 samples Consistent Reliable results, time and time again Convenient Save time with automated instrument setup, sample labeling, and data acquisition Cutting-edge Rare cells analysis easily accomplished with the MACSQuant Column Miltenyi Biotec B.V. Postbus AD Utrecht The Netherlands macs@miltenyibiotec.nl Customer service Netherlands Phone Fax Customer service Belgium Phone Fax Customer service Luxembourg Phone Fax Unless otherwise specifically indicated, Miltenyi Biotec products are for research use only and not for diagnostic or therapeutic use. MACSQuant and MACS are registered trademarks of Miltenyi Biotec GmbH. Copyright 2010 Miltenyi Biotec. All rights reserved.

3 IMMUNOLOGY2011 We set out to bring you superior performance from a flow cytometer. Attune AC O U S T I C FO C U S I N G CY TO M ET E R Save the Date! May 13 17, 2011 San Francisco, California Jolene Bradford Associate Director Research & Development Flow Cytometry Systems We succeeded. The new Applied Biosystems Attune Acoustic Focusing Cytometer is unlike traditional flow cytometers. It uses high-frequency sound waves to precisely focus cells before they pass through the point of interrogation. Now get: ǩ Amazing separation of signal peaks with more precise measurement ǩ Better rare-event detection, up to 20 million counts per run ǩ Rapid sample rates without sacrificing data quality Find out how acoustics is revolutionizing cytometry. Visit us at For research, forensic or paternity use only. Not intended for any animal or human therapeutic or diagnostic use. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners Life Technologies Corporation. The American Association of Immunologists

4 New BD LSRFortessa Cell Analyzer THE journal OF Immunology Introducing the VOL. 185 NO. 3 August 1, 2010 Pages Introducing the BD LSRFortessa The ultimate tool for choice, power, and consistency in flow cytometry, the BD LSRFortessa cell analyzer is an affordable solution, innovatively designed to meet your ever-advancing needs. Available with up to 4 lasers including blue, red, violet, and ultraviolet, the instrument can accommodate the detection of up to 18 colors simultaneously, ready to meet assay requirements today and tomorrow. Of course, the BD LSRFortessa comes with innovation built in. Using patented technologies such as the gel-coupled cuvette and octagon and trigon detection systems, you get unrivaled sensitivity and resolution in a benchtop-sized device. And if you need a custom configuration to meet advanced requirements, the BD special order program offers an unparalleled range of color, wavelength, and power choices. Find out how you can keep your lab in a perpetual state of the art at bdbiosciences.com/fortessa. Innovation is built in. You can depend on it. bdbiosciences.com/fortessa Class I (1) Laser Product. For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company BD BD Biosciences 2350 Qume Drive San Jose, CA bdbiosciences.com Volume 185 l No. 3 l August 1, 2010

5 Get a GRIP: An AAI program designed to help new investigators prepare their NIH grant proposals AAI is pleased to offer a program to match new PI s with established PI s who have significant, successful grant writing careers. The Grant Review for Immunologists Program (GRIP) invites new PI s to submit an outline or NIH-style abstract to the GRIP coordinator who, with the assistance of a small volunteer subcommittee, will attempt to match the topic of the proposal with the research experience of an established PI. Matches will be made as quickly as possible to allow new PI s to meet upcoming NIH grant deadlines. Participation is strictly voluntary and is not intended to supplant internal mentoring programs. GRIP is now accepting both new PI and established PI participants. Please send your CV and a brief description of either your potential research project (new PI s) or grant reviewing experience (established PI s) to infoaai@aai.org (please write GRIP in the subject line). Program details at The Next Generation of Microplates Introducing the Optimiser, a revolutionary breakthrough in microtiter plate technology from Siloam Biosciences, Inc. The Optimiser provides the versatility to extend the value of your samples, maximize the sensitivity of your assays and increase the throughput of your lab with decreased assay time. Compare Optimiser to Traditional 96 Well Plates Optimiser 96 Well Sample volume* 5 μl μl Assay time < 30 min < 10 pg/ml 4-12 hrs ~150 pg/ml Wash cycle NO traditional wash Assay reagent cost*** $5.38/plate 3x wash/step $71/plate Sensitivity** * Min vol is 5 μl; extra vol for high sensitivity mode ** For off-the-shelf IL-6 assay with 100 μl sample volume *** For sandwich assay with chemiluminescence detection Siloam Biosciences, Inc. 417 Northland Blvd Cincinnati, OH USA PH Fax

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