Medicinal Chemistry. Antiviral Agents

Transcription

1 Medicinal Chemistry Antiviral Agents

2 Classical antiviral agents More than 50 years have elapsed since the discovery of the first antiviral agents, i.e. methisazone and 5-iodo-2 -deoxyuridine. In contrast to the evolution in other fields, the antiviral chemotherapy has evolved very slowly at the start. The reasons therefore are multiple: close association between the replicative cycle of the virus and the metabolism of the cell; the intracellular location of the virus; viruses possess considerable fewer virus-associated or -encoded enzymes than bacteria; effective vaccines have been developed for the prevention of some severe viral infections; and antiviral research is a high-risk enterprise for industry. However, the interest in antiviral chemotherapy has been boosted considerably since the identification of HIV (human immuno deficiency virus) as the causative agent of the acquired immune deficiency syndrome (AIDS). Medicinal Chemistry Page 2

3 Ribavirin o Q 1-10-r--' OH o <X:~H N N~ - o (JC; H Il-Pl& K.r Q p N. OH Medicinal Chemistry Page 18

4 Classical antiviral agents 5-Iodo-2 -deoxyuridine (IdUrd, IDU) (16.1) has a structure that is very similar to that of the natural nucleoside thymidine (16.2). The van der Waals radius of an iodo group is somewhat larger than that of a methyl group, and the pka of 5-iodouracil, the 6-membered heterocyclic unit of 16.1, is about 1.5 units lower than that of thymine, the corresponding heterocyclic unit of This results from the inductive effect of the iodo group in the 5-position. These slight differences are apparently sufficient for IdUrd to become a rather selective antiviral agent. W.H. Prusoff synthesized IdUrd first in 1959 by iodination of 2 -deoxyuridine with iodine/nitric acid. IdUrd is active against the multiplication of Herpes simplex virus type 1 (HSV-1), Herpes simplex virus type 2 (HSV-2) and vaccinia virus (VV) in υitro and has proven efficacious in the treatment of herpes eye infections (i.e. herpetic keratitis). Its toxicity, however, does not allow systemic use. Medicinal Chemistry Page 4

5 Classical antiviral agents Also there is a great resemblance in the structures of thymidine and 3 -azido-3 - deoxythymidine (zidovudine, AZT) (16.3). Here, the 3 -position is substituted with an azido group. This compound was the first to be approved by the FDA for the treatment of AIDS patients. J.P. Horwitz synthesized it first in 1964 starting from thymidine. Also, the modes of action of IdUrd and zidovudine are quite similar. They have to be metabolized intracellularly to their 5 -triphosphate derivatives and these triphosphates then interact with (viral) DNA synthesis. These two nucleoside analogs (IDU and AZT), which resemble very well their natural counterpart, have had a tremendous impact on antiviral research. IDU has long been a model compound for the design of new and more selective antiherpes agents. The advent of AIDS, the identification of a retro virus as the causative agent of the disease and the observation that its replication can be blocked by simple nucleosides, gave an important incentive to the search for new antiviral agents. We will see how selectivity can be obtained by interference with virus specific targets. The identification of specific, virus-encoded, enzymes has proved the key step in the design of new antiviral compounds. In the design of new nucleoside analogs, targeted at viral DNA synthesis, the discovery of HPMPC [(S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine] and PMPA [(R)-9-(2- phosphonylmethoxypropyl)adenine] as potent inhibitors of CMV (cytomegalo virus) and HIV, respectively, could be considered as important progress. Medicinal Chemistry Page 5

6 Classical antiviral agents HPMPC PMPA Their antiviral activity clearly indicates that mimicking nucleoside metabolites, e.g. nucleoside monophosphates, can overcome at least the first step of intracellular phosphorylation. It also proves that such phosphonate analogs can be taken up by the cell sufficiently well to exhibit their antiviral action. This brings us one step nearer to the target (viral DNA) site. Medicinal Chemistry Page 6

7 Classical antiviral agents Nucleosides are naturally occurring molecules, which play a crucial role in cell multiplication and function. As a consequence, cells contain a whole battery of enzymes for the anabolism and catabolism of nucleosides. All of these enzymes are potential targets for the action of the modified nucleosides, and this can lead to premature death of the cell. Especially the interaction of the inhibitor with normal cellular DNA may be hazardous in that it could lead to mutagenicity, carcinogenicity or teratogenicity. Moreover, good in vitro antiviral activity not necessarily predicts equivalent in vivo activity. These considerations make the design of new nucleoside antivirals both a difficult and challenging task. Medicinal Chemistry Page 7

8 Base-modified pyrimidine nucleosides as antiherpes agents The intracellular metabolism and mode of action of IdUrd can be presented as follows: I. IdUrd can be phosphorylated by both cellular and virus-encoded thymidine kinases. II. However, IdUrd is phosphorylated more efficiently by the HSVencoded thymidine kinase than by the cellular thymidine kinase, which explains its (modest) selectivity as an antiherpes agent. IdUrd 5 -monophosphate is then phosphorylated to the diphosphate. III. and triphosphate. IV. IdUrd can be incorporated in both cellular and viral DNA. This incorporation impairs the subsequent transcription and replication processes and is believed to be the major reason for the activity and toxicity of IdUrd. As also evident from the above reaction scheme (Scheme 16.1), IdUrd (16.1) is a substrate for thymidine phosphorylase (iv) and for thymidylate synthase V. Both processes lead to deactivation (of IdUrd). Together with the feedback inhibition of the phosphorylated products on the regulatory enzymes of nucleotide biosynthesis, the general biochemical reaction scheme as depicted for IdUrd also holds for most other pyrimidine nucleoside analogs, and could explain their antiviral activity and toxicity. Medicinal Chemistry Page 8

9 Base-modified pyrimidine nucleosides as antiherpes agents A crucial enzyme in the anabolism of pyrimidine 2 -deoxynucleosides is the thymidine kinase, which phosphorylates the nucleoside to its 5 -monophosphate derivative. Some herpes viruses (i.e. HSV-1, HSV-2) and also Varicella-zoster virus (VZV) encode for their own thymidine kinase. Introduction of a substituent in the 5-position of the pyrimidine ring has led to compounds with higher affinity for the virusencoded enzyme than for the cellular enzyme, and thus greater selectivity as antiviral agents. Pertinent examples of this second generation of antiviral compounds are 5-ethyl-2 -deoxyuridine (EtdUrd) and, even more so, 5-(E) bromovinyl-2 - deoxyuridine (BVdUrd, BVDU). Medicinal Chemistry Page 9

10 Base-modified pyrimidine nucleosides as antiherpes agents There is a marked difference in the phosphorylation capacity of the thymidine kinases of different herpes viruses. While the HSV-1-encoded thymidine kinase is capable of converting BVdUrd to its 5 - monophosphate and further onto its 5 -diphosphate, the HSV-2-encoded thymidine kinase is unable to further phosphorylate BvdUrd monophosphate onto its diphosphate. This differential behavior in phosphorylation may explain the differences found in the activity of BVdUrd against HSV-1 and HSV-2. The mode of binding of BVdUrd to the HSV-1 thymidine kinase has been determined. The 3-NH and 4-CO groups of the pyrimidine moiety interact with Gln-125. The bulky 5-substituent occupies a pocket available in the neighborhood of residues Trp-88, Tyr-132, Arg- 163 and Ala-167. A change in conformation of one residue (Tyr-132) is needed for accommodation of the bromovinyl group. The binding mode of the deoxyribose moiety of BVdUrd is similar to that of dthd. Medicinal Chemistry Page 10

11 Base-modified pyrimidine nucleosides as antiherpes agents The BVdUrd is superior in potency to any other antiherpes agent against both HSV-1 and VZV infections, i.e. it is 1000-fold more active in υitro against VZV than acyclovir, the most commonly used drug for the treatment of VZV infections. The VZV is responsible for primary (varicella or chickenpox) and recurrent (zoster and shingles) infections, following reactivation of the virus. BVdUrd (brivudin) is currently the most potent antiviral agent on the market for the treatment of VZV infections. EtdUrd BvdUrd/BVDU 5-F-2 -deoxyuridine 5-F3Me-2 -deoxyuridine Medicinal Chemistry Page 11

12 Sugar-modified purine nucleosides 9-(β-D-Arabinofuranosyl)adenine (ara-a) is a naturally occurring nucleoside, which was synthesized 8 years before it was isolated. Ara-A is an antiviral agent with a multiple mode of action. Theoretically, drugs that have multiple modes of action are most likely to avoid drug-virus resistance but, they may also have the highest risk for toxic side effects. The relative role of the different actions in the overall antiviral activity of ara-a is not well known. Ara-A is phosphorylated to its monophosphate and further to its di- and triphosphate. This triphosphate inhibits DNA polymerases, which could explain the activity of ara-a against DNA viruses. Ara-A can also be incorporated into both host cell DNA and viral DNA. Further-more, ara-a inhibits methyltransferase reactions presumably through inhibition of 5-adenosylhomocysteine hydrolase and accumulation of S- adenosylhomocysteine. The latter acts as a product inhibitor of transmethylation reactions such as those involved in the maturation of viral mrna. Ara-A has been used for the treatment of HSV-1 encephalitis and Herpes zoster in immunocompromised patients, but is now surpassed by acyclovir for this purpose. A major disadvantage of ara- A is that it is promptly deaminated in υiυo by adenosine deaminase, converting the amino group into an oxo group. The resulting hypoxanthine analog has markedly reduced antiviral activity as compared to ara-a. Medicinal Chemistry Page 12

13 Sugar-modified purine nucleosides The search for inhibitors of the adenosine deamination reaction has led to the discovery at the Wellcome Research Laboratories of 9-(2-hydroxyethoxymethyl) guanine (acyclovir) as an antiviral agent. This compound, whose action is surprisingly similar to that of the aforementioned pyrimidine nucleoside analogs, has oriented research in the direction of the acyclic nucleoside analogs. This research has yielded a number of active congeners, i.e. 9-(1,3-dihydroxy2-propoxymethyl)guanine (ganciclovir) and penciclovir. As of today, acyclovir has remained the gold standard for the treatment of HSV infections, whereas ganciclovir is used in the treatment of CMV infections, and both penciclovir (as its prodrug form, famciclovir) and acyclovir (as its prodrug form, valaciclovir) for the treatment of VZV infections. Medicinal Chemistry Page 13

14 Sugar-modified purine nucleosides All of these compounds can be considered as analogs of 2 -deoxyguanosine or carbocyclic 2 -deoxyguanosine (υide infra) from which the 2 -carbon (ganciclovir, penciclovir) or both the 2 - and 3 -carbons (acyclovir) have been deleted. The antiviral activity of acyclovir was discovered by accident, whereas ganciclovir was the result of a structure-activity design starting from acyclovir as the model compound. From a structural viewpoint, ganciclovir is more closely related to 2 -deoxyguanosine than is acyclovir. The antiviral activity of acyclovir can be explained by the same biochemical reaction scheme as presented for IdUrd. There are, however, subtle differences that explain the greater selectivity of acyclovir. Acyclovir is phosphorylated to its monophosphate by a virusspecific thymidine/deoxycytidine kinase, which actually recognizes acyclovir as a deoxycytidine analog. Viruses, which encode for such an enzyme (HSV-1, HSV-2, VZV, but not CMV) are susceptible to the antiviral action of acyclovir. Although the natural substrates for this enzyme are pyrimidine nucleosides, it apparently accepts purine derivatives as substrates. The structure of the complex of HSV-1 thymidine kinase with acyclovir, ganciclovir and penciclovir has been determined by X-ray crystallography. The guanine moiety of all three compounds lay in a similar location, with hydrogen bond pairing being made with Gln-125 via the 1-NH and 6-CO groups. In uninfected cells, phosphorylation occurs to a limited extent. The monophosphate of acyclovir is phosphorylated to the diphosphate by GMP kinase and further to its triphosphate by various cellular enzymes. Medicinal Chemistry Page 14

15 Sugar-modified purine nucleosides The triphosphate of acyclovir is a competitive inhibitor of dgtp for the viral DNA polymerase and can also function as a substrate resulting in the incorporation of acyclovir into DNA and chain termination. Acyclovir is given orally or intravenously in the treatment of HSV and VZV infections, and topically in the treatment of HSV infections (i.e. herpetic keratitis and herpes labialis). For oral use, acyclovir, because of its limited oral bioavailability is now substituted by its oral prodrug form, valaciclovir. As compared to acyclovir, ganciclovir is more easily phosphorylated in CMV infected cells by the virus-encoded (UL 97) protein kinase and its triphosphate has a fivefold greater affinity than ACV (acyclovir) triphosphate for CMV DNA polymerase. Ganciclovir can be incorporated both internally and at the 3 -terminal end of DNA. Ganciclovir is active against HSV-1, HSV-2, VZV, CMV and EBV. It is fairly toxic for the bone marrow (neutropenia). Its clinical use is restricted to the treatment of CMV infections in immunocompromised patients. Penciclovir has the same antiviral spectrum as acyclovir. As compared to acyclovir, penciclovir leads to higher triphosphate concentrations in virus-infected cells and its antiviral activity persists for a longer time after removal of the compound. In fact, after removal of acyclovir, antiviral activity rapidly disappears. Not only penciclovir, but also BVdUrd and ganciclovir show persist-ent antiviral activity after the drugs have been removed from the medium. This is due to the greater stability of their triphosphates as compared to that of acyclovir triphosphate. Medicinal Chemistry Page 15

16 Sugar-modified purine nucleosides (Synthesis of Acyclovir) Acyclovir, 2-amino-1,9-dihydro-9-[(2-hydroxyethoxy)methyl]-6H-purin-6-one (36.1.5), is synthesized by alkylating guanine with 1-benzoyloxy-2-chloromethoxyethane in triethylamine. The hydroxyl and amino groups of guanine are previously protected with a trimethylsilyl group by being treated with hexamethyldisilazane. After hydrolysis the resulting product with water, 9-(2-benzoyloxymethoxymethyl)guanine (36.1.4) is isolated. Treating this with a methanol solution of ammonia removes the benzoyl protecting group from the hydroxyethoxymethyl fragment, giving acyclovir. Medicinal Chemistry Page 16

17 Anti-HCV - Ribavirin In contrast with the preceding compounds, ribavirin has a broad spectrum activity against RNA and DNA viruses both in υitro and in υiυo. R.K.Robins first synthesized ribavirin. The structural requirements for the broad-spectrum antiviral activity of ribavirin are very stringent. The compound shows its greatest potency against myxo (influenza) and paramyxo (respiratory syncytial) virus infections. Ribavirin also shows activity against some hemorrhagic fever viruses such as Lassa, Machupo, Pichinde, Rift Valley and Hantaviruses. Therapeutic efficacy has been demonstrated with ribavirin, given as a small-particle aerosol, in infants suffering from respiratory syncytial virus (RSV) infection. The compound has been approved for the treatment of RSV infections, as well as human hepatitis C virus (HCV) infections, for the latter only in combination with interferons. The mode of action of ribavirin is multipronged and may also vary from one virus to another. Ribavirin (5 -monophosphate) can be considered as an analog of AICAR which is a precursor of both AMP and GMP. As has been elucidated by X-ray crystallography studies, there is a nice resemblence between ribavirin and guanosine; by rotating the amide group, also good resemblence is found between ribavirin and adenosine Medicinal Chemistry Page 17

18 Ribavirin o Q 1-10-r--' OH o <X:~H N N~ - o (JC; H Il-Pl& K.r Q p N. OH Medicinal Chemistry Page 18

19 Synthesis of Ribavirin Ribavirin, 1-β-D-ribofuranosyl-1H-1,2,4-triazol-3-carboxamide ( ), is synthesized by reacting methyl ester of 1,2,4-triazol-3-carboxylic acid with O-1,2,3, 5-tetraacetyl-β-D-ribofuranose to make methyl ester of 1-O-2,3,5-tetraacetyl-β-Dribofuranosyl-1,2,4-triazol-3-carboxylic acid ( ), which is treated with an ammonia solution of methanol to simultaneously dezacylate the carbohydrate part and amidation of the carboxyl part of the product to give ribavirin [29 37]. Medicinal Chemistry Page 19