MIRCEN Activities on Preservation and Utilization of Microbial Resources

Transcription

1 MIRCEN Activities on Preservation and Utilization of Microbial Resources Aparat Mahakhant Microbiological Resources Centre (MIRCEN) Thailand Institute of Scientific and Technological Research (TISTR)

2 Introduction to MIRCEN, TISTR Microbial Culture Collection (Bangkok MIRCEN-UNESCO) 1976 Non-pathogenic: bacteria, yeast, filamentous fungi ACC at TISTR 1995 Biofertilizer Project Useful microbial strains agriculture, industry, environment

3 Functions of MIRCEN, TISTR Services Research - Research relevant to the collection - Applied research on utilization of microbial resources

4 Main services Culture supply Deposit of microorganisms public & safe deposit Preservation of strains Identification of strains Isolation and purification of strains Training service

5 Number of strains available for service Groups Number of strains Algae 429 Bacteria 730 Fungi 383 Yeast 516 Total 2,058

6 Number of strains service Customers: Year Research institute, universities Industries SME setc.

7 Research conducted by MIRCEN, TISTR 1. Research relevant to the collection 1.1 Survey & collection of microbial strains from terrestrial & aquatic ecosystems e.g. gliding bacteria and microalgae. 1.2 Taxonomy of microbial strains using morphology, biochemical test & 16s rdna 1.3 Long-term preservation of microbial strains. esp. microalgae

8 Research conducted by MIRCEN, TISTR (cont.) Development of long-term preservation techniques for microalgae Cryopreservation Gelatin disc Encapsulation Freeze-dried

9 Development of long-term preservation techniques for microalgae (cont.) Viability of microalgal strains Class Characteristics No. of tested Gelatin disc Encapsulation Freeze-dried strains S 1 SM 2 Cyanophyceae Filamentous (N 2 -fixing) Filamentous (non-n 2 -fixing) Chlorophyceae Colony Unicellular Total (0) (11) (9.3) (8) 1 10% skim milk was used as cryoprotectant 2 10% skim milk + 1% monosodium glutamate was used as cryoprotectant 3 ( ) % of survival strains

10 Development of long-term preservation techniques for microalgae (cont.) Viability of microalgal strains after cryopreservation Number of Survival Strains Class Characteristics No. of tested strain 24 hr 12 m 10%G 1 10%M 2 5%D 3 10%D 10%G 10%M 5%D 10%D Cyanophyceae Filamentous (N 2 -fixing) Filamentous (non-n 2 -fixing) Chlorophyceae Colony Unicellular Total (95) 4 (100) (100) (99) (80) (74) (82) (84) 1 glycerol, 2 methanol, 3 dimethyl sulfoxide, 4 ( ) % of survival strains

11 Research conducted by MIRCEN, TISTR 2. Applied research Agriculture Biofertilizer Alginure Algotech Co., Ltd. Screening of N 2 -fixing BGA tolerant acid soil saline soil arid area

12 2. Applied research Agriculture Agroactive compound insecticide Cotton ball worm & Beet army worm Hapalosiphon TISTR 8252 Hapalindole

13 2. Applied research Agriculture Agroactive compound fungicide Calothrix sp. TISTR 8906 puwainaphycin

14 2. Applied research Agriculture Agroactive compound plant hormone Calothrix sp. TISTR 8906

15 2. Applied research Agriculture Soil conditioner

16 2. Applied research Food Hed Lap Nostoc commune Nutritional Value moisture 10.19% protein 20.26% fat 0.02% dietary fiber 43.00% calcium 3.55% ash 16.20% iron 0.28% vitamin A 2.31% etc. (incl. microbial, heavy metals, toxicity)

17 2. Applied research Food (cont.) Food products from Hed Lap IP -1 patent - 9 pitty patents

18 2. Applied research Paint Industry Research on the establishment of Thailand standard test method for determining the resistance of exterior emulsion paint to algae On-site arid mountainous Laboratory industrial rubber tree Phormidium angustissimum TISTR 8979 Nostoc paludosum TISTR 8978 Chlorococcum sp. TISTR 8973 seaside

19 Research conducted by MIRCEN, TISTR (cont.) 2. Applied research - Environment Biocontrol of toxic algal blooms in freshwater by freshwater calm (Chamberlainia hainesiana) Microcystis aeruginosa at 10 3 cells/ml Removal > 90% large size (20 cm) > 80% small size (10 cm)

20 Race-way ponds 10,000-25,000 L

21 Thank you very much Center for Research and Development Strategy (CRDS) Japan Science and Technology Agency (JST) Prof. Dr. Mokoto M. Watanabe, NIES All the audiences