Your single-source laboratory solution. FISH Probe Library
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1 Your single-source laboratory solution. FISH Probe Library
2 Alphabetical List by Probe with Clinical Indication Probe Clinical Indication 1p36 (TP73)/19q13 (GLTSCR) 1q21 (CKS1B) 5q (CSF1R/RPS14) 7q (MDFIC) 13q14 (DLEU) 20q (PTPRT) 21q ALK ATM BCL2 BCL6 CBFB, inv(16) CCND1 (CYCLIN D1) CCND1/IgH, t(11;14) CDKN2A (p16) (9p21) CKS1B (1p/1q ) Centromere 4 Enumeration Probe Centromere 6 Enumeration Probe Centromere 7 Enumeration Probe Centromere 8 Enumeration Probe Centromere 9 Enumeration Probe Centromere 10 Enumeration Probe Centromere 12 Enumeration Probe Centromere 15 Enumeration Probe Centromere 17 Enumeration Probe DDIT3 (CHOP) EGFR ETV6/RUNX1 (TEL/AML), t(12;21) EWSR1 FGFR1 Follicular lymphoma, Oligodendroglioma AML, MDS AML, MDS, MPN, CLL, MDS, MPN MDS, MPN B-ALL (adult), AML, T-cell anaplastic lymphoma CLL B-ALL (adult and pediatric), AML, CML AML CLL, Mantle cell lymphoma, CLL, Mantle cell lymphoma, B-ALL (adult), Melanoma MDS, MPD, MPN MPN, CLL Myxoid/Round cell liposarcoma Glioma, B-ALL, iamp ALL Ewing sarcoma, PNET 1
3 Probe Clinical Indication FGFR3/IGH, t(4;14) FOXO1 (FKHR) HER2 IGH IGH/BCL2, t(14;18) IGH/MAF, t(14;16) IGH/MYC, t(8;14) MALT1, t(11;18) MECOM (EVI1) MET MYB MYC MYCN PDGFRA PDGFRB PML/RARA, t(15;17) PTEN RARA RB1 (13q14) RET ROS1 RREB1 RUNX1T1/RUNX1 (ETO/AML), t(8;21) SS18 (SYT) TCF3 (E2A) TCR TP53 (17p deletion) UroVysion XX/XY Alveolar rhabdomyosarcoma Breast, Gastric cancer B-cell lymphoma Burkitt lymphoma, High grade B-cell lymphoma B-ALL (adult and pediatric), AML Marginal zone lymphoma AML, MDS Melanoma Burkitt lymphoma, High grade B-cell lymphoma Neuroblastoma APL Glioma APL Retinoblastoma Melanoma AML Synovial sarcoma T-cell leukemia AML, CLL, MDS, Bladder cancer Bone marrow transplant *includes Multiple myeloma and MGUS 2
4 Hematology/Oncology Profiles Disease Probes Included B-ALL (Adult) B-ALL (Pediatric) AML CLL MDS MPN/CML MPN with Eosinophilia Multiple Myeloma Aggressive B-cell Lymphoma 21q Chromosome 6 MYC CDKN2A (p16) Centromere 4 Centromere 10 Centromere 17 ETV6/RUNX1, t(12;21) TCF3 (E2A) 5q 7q CBFB, inv(16) PML/RARA, t(15;17) RUNX1T1/RUNX1, t(8;21) 13q14 (DLEU) ATM (11q) CCND1/IGH, t(11;14)** Centromere 12 TP53 (17p) 5q 7q 20q Centromere 8 13q14 (DLEU) 20q CDKN2A (Centromere 9) Centromere 8 FGFR1 PDGFRA PDGFRB 13q14 (DLEU) CCND1/IGH, t(11;14) Centromere 7 Centromere 9 Centromere 15 CKS1B (1q21) FGFR3/IGH, t(4;14) IGH/MAF, t(14;16) TP53 (17p) BCL2 BCL6 MYC **used as differential 3
5 Solid Tumor Probes Disease Probe Breast Cancer HER2 Bladder Cancer UroVysion Colorectal Cancer Gastric Cancer Glioma Lung Cancer (Non-Small Cell) Melanoma Neuroblastoma Retinoblastoma EGFR HER2 1p/19q EGFR PTEN ALK MET RET ROS1 CCND1 Centromere 6 MYB RREB1 MYCN RB1 (13q14) Sarcomas Ewing Sarcoma Alveolar Rhabdomyosarcoma Synovial Sarcoma Myxoid/Round Cell Liposarcoma EWSR1 FOXO1 (FKHR) SS18 (SYT) DDIT3 (CHOP) 4
6 Probes Suitable for FFPE Specimens Probe Clinical Indication 1p36 (TP73)/19q13 (GLTSCR) ALK BCL2 BCL6 CBFB, inv(16) CCND1 (CYCLIN D1) CCND1/IgH, t(11;14) DDIT3 (CHOP) EGFR ETV6/RUNX1 (TEL/AML), t(12;21) EWSR1 FOXO1 (FKHR) HER2 IGH IGH/BCL2, t(14;18) IGH/MYC, t(8;14) MALT1, t(11;18) MET MYC MYCN PDGFRA PDGFRB PTEN RB1 (13q14) RET ROS1 SS18 (SYT) TCR XX/XY Follicular lymphoma, Oligodendroglioma, T-cell anaplastic lymphoma B-ALL (adult and pediatric), CML AML CLL, Mantle cell lymphoma, CLL, Mantle cell lymphoma, Myxoid/Round cell liposarcoma Glioma, B-ALL Ewing sarcoma, PNET Alveolar rhabdomyosarcoma Breast, Gastric cancer B-cell lymphoma Follicular lymphoma Burkitt lymphoma, High grade B-cell lymphoma B-ALL (adult and pediatric), AML Marginal zone lymphoma Burkitt lymphoma, High grade B-cell lymphoma Neuroblastoma Glioma Retinoblastoma Synovial sarcoma T-cell leukemia Bone marrow transplant Formalin-fixed, paraffin-embedded *includes Multiple myeloma and MGUS 5
7 Specimen Requirements Type Preferred Alternate Blood Optimal: 10 ml (adult) or 3-5 ml (pediatric) in green-top (heparin) tube Acceptable: Lavender-top (EDTA) tube Bone Marrow Optimal: 1-3 ml in green-top (heparin) tube Fixed-cell pellet from cytogenetic analysis Slides prepared from a cytogenetic analysis Fixed-cell pellet from cytogenetic analysis Acceptable: Lavender-top (EDTA) tube Slides prepared from a cytogenetic analysis Tissue Formalin-fixed, paraffin-embedded (FFPE) tissue block 4-5 unstained slides, cut to 4-5 microns thick, air-dried overnight Specimens should be maintained at room temperature. For more specific specimen requirements for individual tests, please refer to 6
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