Curriculum Vitae. Pittsburgh, Pittsburgh, PA USA

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1 Curriculum Vitae Personal Particulars Name: Contact information: Voice Mail: Fax: Education Nov : Aug June Nov May 1997 Sep July 1995: Sep July 1992: Wen-Xing Ding, MD, PhD, University of Pittsburgh, Pittsburgh, PA USA (412) (office) (724) (home) wed1@pitt.edu wxding@hotmail.com (412) (office) Post-doctoral research fellow Pittsburgh, PA 15261, USA (Mentor: Professor Xiao-Ming Yin) Ph.D. (Molecular Toxicology) Department of Community, Occupational and Family Medicine National University of Singapore, Singapore (Mentor: Professor Choon-Nam Ong) China Medical Board, New York (CMB) Fellow, Department of Community, Occupational and Family Medicine National University of Singapore, Singapore M. Med (Master of Medicine on Environmental Toxicology) Shanghai Medical University, China (Mentor: Professor Hui-Gang Zhu) B. Med. (Bachelor of Medicine on Preventive Medicine) Shanghai Medical University, China Professional Position and Working Experience July 2005-present: Research Assistant Professor Pittsburgh, PA 15261, USA (Projects: (1) Studies on the role of autophagy in alcohol-induced liver injury; (2) Studies on mitochondrial autophagy) Nov : Research Associate Pittsburgh, PA 15261, USA 1

2 (Project: Studies on proteasome inhibitors-induced apoptosis and autophagy in cancer therapy) Nov : Aug to Nov 2001: Jul to Jul. 1998: Post-doctoral research fellow Pittsburgh 15261, USA (Project: Studies on death-receptor activation-induced apoptosis in hepatocytes and liver) Ph.D. student Dept. of Community, Occupational and Family Medicine Faculty of Medicine, National University of Singapore (Project: Studies on the hepatotoxicity of cyanobacterial toxinmicrocystin) Research and Teaching Assistant Dept. of Environmental Health, School of Public Health, Shanghai Medical University, P.R. China (Project: Studies on the genotoxicity and mutagenicity of water pollutants from Changjiang River in China) Teaching experience and interest Jul to Jul Undergraduate student class: Environmental Toxicology Currently Teaching Interest: Mitochondrial apoptotic pathway and cell death Autophagy and cell death Honors and Awards: 1995 Science Technology Progress Awards by Shanghai Municipality, Shanghai, P.R. China 1996 Science Technology Progress Awards by Shanghai Municipality, Shanghai, P.R. China 1997 Research Scholarship, National University of Singapore, Singapore 2000 DSO Top-up Scholarship, National University of Singapore, Singapore 2003 The Pathology Post-doctoral Research Training Program (PPRTP), Department of Pathology, 2003 American Liver Foundation Postdoctoral Fellowship 2004 Third Place Award for the oral presentation at Annual Research Retreat, 2005 Liver Scholar, American Liver Foundation 2

3 Service Activities Grant Reviewer, The International Foundation for Science (IFS), October 2007 Journal Reviewer: Ad hoc reviewer for Hepatology, Free Radical Research, Toxicology, Environmental Toxicology, Journal of Biological Chemistry, Liver International, World Journal of Gastroenterology, Apoptosis Other Experience and Professional Memberships Cell Death Society 2002 American Association for the Studies of Liver Disease (AASLD ) 2004 Recent Publications (since 1998) 1. Ding, W.X., Shen, H.M., Shen, Y., Zhu, H.G. and Ong, C.N. (1998) Microcystic cyanobacteria caused mitochondrial membrane potential alteration and reactive oxygen species formation in primary cultured rat hepatocytes. Envrion. Health Perspect. 106: Ding, W.X., Shen, H.M., Zhu, H.G. and Ong, C.N. (1998) Studies on oxidative damage induced by cyanobacteria-extract in primary cultured rat hepatocytes. Environ. Res. 78: Ding W.X., Shen H.M., Zhu H.G. and Ong C.N. (1999) Evaluation of the genotoxicity and mutagenicity of microcystic cyanobacteria from China. Mutat. Res. 442: Xu, J.K., Liu, Y.F., Zhu, H.G. and Ding, W.X. (2000) The identification and toxicity of the microcystin in the waterbloom obtained from an eutrophied lake. Biomed Environ Sci.13: Ding, W.X., Shen, H.M. and Ong, C.N. (2000) Microcystic cyanobacteria extract induces cytoskeletal disruption and intracellular glutathione alteration in hepatocytes. Environ. Health Perspect. 108: Ding, W.X., Shen, H.M. and Ong, C.N. (2000) The critical role of ROS and mitochondrial membrane permeability in microcystin-lr induced rapid apoptosis in primary rat hepatocytes. HEPATOLOGY 32: Shen, H.M., Yang, C.F., Ding, W.X., Liu, J., and Ong, C.N. (2001) Oxidative stress mediated apoptotic signaling pathway in sodium selenite-treated HepG2 cells: mitochondria serve as the main target. Free Radic. Biol. Med 30: Ding, W.X., Shen, H.M. and Ong, C.N. (2001) Pivotal role of mitochondrial Ca(2+) in microcystin-induced mitochondrial permeability transition in rat hepatocytes. Biochem Biophys Res Commun. 285: Ding, W.X., Shen, H.M. and Ong, C.N. (2001) Critical role of reactive oxygen species formation in microcystin-induced cytoskeleton disruption in primary cultured hepatocytes. J Toxicol Environ Health A. 64: Ding, W.X., Shen, H.M. and Ong, C.N. (2002) Calpain Activation after Mitochondrial Permeability Transition in Microcystin-Induced Cell Death in Rat Hepatocytes. Biochem Biophys Res Commun. 291: Shen, H.M., Ding, W.X. and Ong, C.N. (2002) Intracellular glutathione is a cofactor in methylseleninic acid-induced apoptotic cell death of human hepatoma HepG2 cells. Free Radic. Biol. Med. 33:

4 12. Ding, W.X*. and Ong, C.N. (2003) Role of oxidative stress and mitochondrial changes in cyanobacteria-induced apoptosis and hepatotoxicity. FEMS Microbiol Lett. 220:1-7. Invited review. * correspondence author 13. Zhao, Y, Ding, W.X*, Qian T, Watkin S, Lemasters J.J. and Yin, X.M. (2003) Bid activates multiple mitochondrial apoptotic mechanisms in primary hepatocytes after death receptor engagement. Gastroenterology 125: *Co-first author. 14. Yin X.M. and Ding W.X. (2003) Death Receptor Activation-Induced Hepatocytes Apoptosis and Liver Injury. Current Molecular Medicine. 3: Kim, T.H.*, Zhao, Y*, Ding, W.X*., Shin, J.N., He, X., Seo Y.W., Chen, J., Rabinowich H., Amoscato, A. and Yin, X.M. (2004) Bid-cardiolipin interaction at the mitochondria contact site contributes to mitochondria cristae reorganization. Mol Biol Cell. 15: *Co-first author. 16. Ding, W.X, Ni, H.M., Defrancesca, D., Stolz, D.B., and Yin, X.M (2004) Bid-Induced Oxygen Radicals Promote Mitochondrial Dysfunction, Caspase Activation and Hepatocyte Apoptosis. HEPATOLOGY 40: Ding, W.X., and Yin X.M. (2004) Dissection of the multiple mechanisms of TNF-alphainduced apoptosis in liver injury. J Cell Mol Med Oct-Dec;8(4): Chen X, Ding, W.X. Ni, H.M., Gao, W., Shi, Y.H., Gambotto, A.A., Fan, J., Beg, A.A. and Yin, X.M. (2007) Bid-independent Mitochondria Activation in TNF{alpha}-induced Apoptosis and Liver Injury. Mol Cell Biol. 27(2): Ding, W.X. Ni, H.M., Gao, W., Hou, Y.F., Melan, M.A., Chen X, Stolz, D.B., Shao, Z.M., and Yin, X.M. (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J Biol Chem. 282(7): Ding, W.X., Ni, H.M, Chen, X., Yu, J., Zhang, L., and Yin, X.M. (2007) A coordinated action of Bax, PUMA, and p53 promotes MG132-induced mitochondria activation and apoptosis in colon cancer cells. Mol Cancer Ther. 6(3): * Cover story. 21. Ding, W.X. Ni, H.M., Gao, W., Yoshimori, T., Stolz, D.B., Ron, D., and Yin, X.M. (2007) Linking of Autophagy to Ubiquitin-Proteasome System Is Important for the Regulation of Endoplasmic Reticulum Stress and Cell Viability. Am J Pathol. 171(2): Ding, W.X*. Ni, H.M.,and Yin, X.M. (2007) Absence of bax switched MG132-induced apoptosis to non-apoptotic cell death that is suppressed by transcriptional and translational inhibitors. Apoptosis. * correspondence author. 23. Ding, W.X., and Yin, X.M. (2008) Sorting, recognition and activation of the misfolded protein degradation pathways through macroautophagy and the proteasome. Autophagy. Mar-Apr;4(2): Yin, X.M., Ding, W.X., and Gao, W. (2008) Autophagy in the liver. Hepatology. May;47(5): Review. 25. Gao, W., Ding, W.X., Stolz, D., and Yin, X.M. (2008) Induction of macroautophagy by exogenously introduced calcium. Autophagy. May 30;4(6). 26. Shi, Y.H., Ding, W.X., Zhou, J., He, J.Y., Xu, Y., Gambotto, A.A., Rabinowich, H., Fan, J. and Yin, X.M. (2008) Expression of X-linked inhibitor-of-apoptosis protein in hepatocellular carcinoma promotes metastasis and tumor recurrence. Hepatology. Aug; 48(2):

5 27. Ni, H.M., Chen, X, Ding, W.X., Schuchmann, M., and Yin, X.M. (2008) Differential Roles of JNK in ConA/GalN and ConA-Induced Liver Injury in Mice. Am J Pathol. Oct;173(4): Ding, W.X., and Yin, X.M. (2008) Analyzing macroautophagy in hepatocytes and the liver. Methods in Enzymology. In press. Publications (in Chinese, before 1998) 1. Ding, W.X., Jiang, S, Yao, J and Zhu, H.G. Studies on Mutagenicity of Water in Changjiang. Shanghai Environmental Sciences (9). 2. Ding, W.X., Nie, N and Zhu, H.G. Transformation of Water Samples in Changjiang River With Syrian Hamester Embryo Cells. China Public Health (6). 3. Ding, W.X., Nie, N and Zhu, H.G. Detection of genotoxicity of water in Changjang River by using UDS test. China Environmental Science (4). 4. Ding, W.X. and Zhu, H.G. The effects of Changjiang water on the micronuclei formation in SHE cells. Shanghai Journal of Preventive Medicine (12). 5. Zhang J., Wu Z., Zhang, Y., Ding, W.X. and Zhu, H.G. Evaluation of Genotoxicity of Source Water and Tap Water Samples by Using Mouse Micronucleus Test. Shanghai Environmental Sciences (2). 6. Zhang J., Wu Z., Zhang, Y., Ding, W.X. and Zhu, H.G. Studies on the mutagenicity of tap water and source water in G city. JOURNAL OF ENVIRONMENT AND HEALTH Ren, T., Ding W..X and Song, H. Analysis of the contamination and toxicity of algae from Dalan lake in Dongguan city. GUANGDONG JOURNAL OF HEALTH AND EPIDEMIC PREVENT (3). 8. Ding, W.X., Shen, H.M., Zhu, H.G and Ong C.N. Hepatotoxicity and Formation of Reactive Oxygen Species Induced by Microcystis Aeruginosa Toxin. Chinese Journal of Preventive Medicine (5). C. Book Chapters Yin, X.M., Ding, W.X. and Zhao, Y.G. Bcl-2 family proteins, the master regulators of apoptosis. Humana Press Inc., Totowa, NJ. 2003: The essential of apoptosis: A guide for Basic and Clinical Research. Edited by Yin, X.M and Dong Z. D. Oral Presentations at International Scientific Conferences 1. Ni, H.M., Chen, X.Y, Ding, W.X.* Yin, X.M. Bid-independent Mitochondria Activation in TNF{alpha}-induced Apoptosis and Liver Injury. Experimental Biology April 28-May 3. Washington DC, USA. Oral presentation*. 2. Ding, W.X., Ni, H.M., Chen X., Stolz, D., and Yin, X.M. Caspase-inhibition switched TNF-alpha-induced apoptosis to autophagic cell death in hepatocytes. Oral presentation at AASLD Annual Meeting 2007, Nov 2-5. Boston, MS, USA. Oral presentation*. 5

6 E. Presentations at International Scientific Conferences 1. Ding, W.X., Shen, H.M. and Ong, C.N. Critical role of mitochondrial Ca 2+ and reactive oxygen species in microcystin-induced mitochondrial permeability transition in rat hepatocytes. 51 st Annual Meeting of American Association for the Study of Liver Diseases (AASLD), October, 27-31, Dallas, Texas. 2. Ding, W.X., Shen, H.M. and Ong, C.N. Regulation of reactive oxygen species production in microcystin treated rat hepatocytes. 7 th Annual Meeting of the Oxygen Society, Novermber 16-20, 2000, San Diego, California. 3. Ding, W.X. and Yin, X.M. Critical role of reactive oxygen species in death-receptor induced apoptosis in primary cultured mouse hepatocytes. Keystone Symposia-Molecular mechanisms of apoptosis (B6). Feb 8-13, Banff, Alberta, Canada. 4. Zhao, Y, Ding WX, Kuharsky D.K., and Yin, X.M. Dissection of Bid-mediated mitochondrial events in primary hepatocytes following death receptor activation. FASEB Office of Scientific Meetings and Conferences. Experimental Biology April 11-15, San Diego, California. 5. Yin X.M, Ding WX. An orchestrated activation of multiple mitochondria apoptotic events by Bid. Oral presentation at Keystone Symposia-Apoptosis in Biochemistry and Structure Biology (J6). Feb 3-8, Keystone, CO, USA. 6. Ding, W.X., Ni, H.M., Defrancesca, D., Stolz, D.B., and Yin, X.M.TNF-α and anti-fas induced reactive oxygen species requires bid for generation and compromise mitochondria structures and functions. Abstract presented at Keystone Symposia- Apoptosis in Biochemistry and Structure Biology (J6). Feb 3-8, Keystone, CO, USA. 7. Ding, W.X. and Yin, X.M. Critical role of Reactive Oxygen Species in the Regulation of Bax Translocation Following Death Receptor Activation June 8-10, Department of Pathology Annual Research Retreat, Seven Spring, Pittsburgh, PA. (Selected as oral presentation and Third Place Award). 8. Ni, H.M., Ding, W.X., Defrancesca, D and Yin, X.M. Bid Dependent Reactive Oxygen Species Production Promotes TNF-α and Anti-Fas-Induced Apoptosis by Multiple Mechanisms. Cell Death. Gordon Research Conference. Aug 29-Sept Big Sky, MT, USA. 9. Ding, W.X., Ni, H.M., Chen X., and Yin, X.M. Critical role of Reactive Oxygen Species in the Regulation of Bax Translocation Following Death Receptor Activation. Abstract presented at AASLD Annual Meeting 2005 Nov 11-15, San Francisco, CA, USA. 10. Yin, X.M., Ding, W.X., Gao, W., Ni, H,M., Chen, X.Y., Stolz, D.B. Control of ER stress by autophagy. Abstract presented at Keystone Symposia April 15-20, Monterey, CA, USA. 11. Ni, H.M., Chen, X.Y, Ding, W.X.* Yin, X.M. Bid-independent Mitochondria Activation in TNF{alpha}-induced Apoptosis and Liver Injury. Experimental Biology April 28-May 3. Washington DC, USA. Oral presentation*. 12. Ding, W.X., Ni, H.M., Chen X., Stolz, D., and Yin, X.M. Caspase-inhibition switched TNF-alpha-induced apoptosis to autophagic cell death in hepatocytes. Oral presentation at AASLD Annual Meeting 2007, Nov 2-5. Boston, MS, USA. Oral presentation*. 13. Ding, W.X., Ni, H.M., Chen X., Gao, W., and Yin, X.M. Simultaneously inhibition of autophagy and proteasome preferentially kills tumor cells. Gordon Research Conference. 6

7 Autophagy in Stress, Development & Disease. January 6-11, 2008 Ventura Beach Marriott Ventura, CA. 14. Ding, W.X., Ni, H.M., Chen X., Gao, W., and Yin, X.M. Suppression of autophagy enhanced proteasome inhibitor-induced cell death selectively in cancer cells. Abstract submitted to Experimental Biology April New Orleans. F. Research Support Completed: Liver Scholar, American Liver Foundation (Salary Support only) 7/01/2005-6/30/2008 American Liver Foundation Post-Doctoral Fellowship 7/01/2003-6/30/2004 Post-Doctoral Training Grant 1/01/ /30/2004 Pending NIH R21 The role and mechanism of mitochondria turn over in alcohol-induced liver injury $22,500 under revision, Project Period Begin Date: 12/01/9 NIH R21 The role of autophagy in alcohol-induced liver injury $22,500, under review, Project Period Begin Date: 07/01/2009 NIH R01 The role of macroautophagy in alcoholic liver injury Project Period Begin Date: 07/01/2009 $125,000, under review, DOD Peer Reviewed Medical Research Program Role of Macroautophagy in Alcohol-induced Liver Injury $196,364, under review Project Period Begin Date: 07/01/