BIO360 Quiz #1. September 14, Name five of the six Hallmarks of Cancer (not emerging hallmarks or enabling characteristics ): (5 points)

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1 Name: BIO360 Quiz #1 September 14, Name five of the six Hallmarks of Cancer (not emerging hallmarks or enabling characteristics ): (5 points) 2. The controversial hypothesis that only a small subset of tumor cells are capable of self-renewal and differentiation into other tumor cell types is called the hypothesis. 3. Immortalized cell lines show which ones of the following traits? Circle all that apply. (1.5 points) (2 points) Anchorage-dependence Has passed the crisis point Causes tumors when injected into an animal Is less-differentiated than cells in an animal Contact inhibition Requires FBS (fetal bovine serum) to grow 4. The image on the right illustrates which one of the following processes? A. Microevolution B. Mitotic recombination C. Gain-of-function mutation D. Focus formation E. Translocation (1.5 points)

2 Name: BIO360 Quiz #2 September 21, By definition, epigenetic changes lead to a phenotype, are not encoded in DNA sequence and (2 points) 2. In a genomic cancer dataset, we find a large number of loss-of-function mutations clustered in one specific gene. This gene would be classified as A. cytogenetic. (2 points) B. a driver. C. a loss of heterozygosity. D. a passenger. E. an oncogene. 3. What is the Restriction Point? (2 points) 4. In the original screen to isolate cdc mutants, why was it essential to look for temperature sensitive mutants? (2 points) 5. The Bäumer paper examines the Inca1 protein. They chose this protein to investigate because they had previously shown that it binds to which other protein? (2 points)

3 Name: BIO360 Quiz #3 September 28, Identify the one statement that correctly identifies a use of each one of the techniques listed below. Each answer may be used once, more than once or not at all. (1 point each) A Used to determine if cells can grow in an anchorageindependent fashion. qrt-pcr B Used to purify an unmodified protein from a cell lysate. GST fusion protein C Used to quantify the amount of protein in a cell lysate. Western blot D Used to quantify the amount of mrna in a cell lysate. Immunoprecipitation E Used to quickly purify fusion proteins on glutathione beads. FACS F Used to determine the genotype of an ES cell line. Southern blot G Used to measure the ability of two proteins to bind each other. H Used to separate cells based on fluorescence. 2. Small GTPase proteins (like Ras) need a second protein to become activated. What is the name of this type of protein? (2 points; the standard abbreviation is acceptable) 3. Which one of the following is NOT a common mechanism of oncogene activation? (2 points) A. Promoter methylation B. Gene mutation C. Gene amplification D. Fusion transcriptions from chromosomal rearrangements E. Novel regulatory sequences from chromosomal rearrangements

4 Name: BIO360 Quiz #4 October 5, A portion of Figure 2 from the Aranda et al. paper is shown, in which FGFR1 is overexpressed to activate a signal transduction pathway. (2 points each) 1A. What is the Flag in this experiment? 1B. Which western blot is a positive control, showing that the Flag-Spry2 protein was successfully immunoprecipitated? 1C. Which western blot is a positive control, showing that the signal transduction pathway was successfully activated? 2. Name the two reasons that make lacz a good reporter gene in human cells. (2 points) 3. Which domain or motif is NOT a common DNA binding domain? (2 points) A. Zinc finger B. Homeodomain C. Helix-loop-helix D. Winged helix (forkhead) domain E. SH3

5 Name: BIO360 Quiz #5 October 12, 2012 Name any one protein from the list below that meets each of the criteria listed. Each answer may be used once, more than once or not at all. 1 point each. This protein is a subunit of TFIID. This protein is a substrate for a HDAC. This protein heterodimerizes with E2F-1, allowing it to bind it s enhancer. When this receptor protein is activated by it s ligand, it leads to the activation of a caspase cascade. Mdm2 binds to this protein and destabilizes it. This protein is a substrate for ATM. This protein is a protease, catalyzing the destruction of other proteins. This protein s gene overlaps the Ink4a gene but is read in an alternative reading frame. When released from mitochondria, this protein binds Apaf-1 to help initiate apoptosis. Inheriting loss-of-function allele in one of two genes encoding this protein causes the disease familial retinoblastoma. Apaf-1 ARF ATM Caspase-9 Cdk1 Cdc25 Cyclin D Cytochrome c DP-1 Fas FasL Histone H3 Mdm2 p53 prb TAF4

6 Name: BIO360 Quiz #6 October 19, Identify the one statement that correctly identifies a use of each one of the techniques listed below. Each answer may be used once, more than once or not at all. (1 point each) A Used to determine if a specific protein binds to a specific region of DNA in vivo. FACS B Used to determine if cells can grow in an anchorageindependent fashion. BrdU labeling C At what subcellular locations is my epitope-tagged protein? Chromatin Immunoprecipitation RNAi (or sirna) D E Used to determine if two proteins bind each other in vivo. Used to separate mature B cells from other cells in a population. Co-immunoprecipitation F Used to purify an unmodified protein from a cell lysate. Immunofluorescence (IF) G Used to decrease (or knock down) expression of a gene. Microarray (or genechip) H Labels all cells that are in S phase. Annexin V staining I Used to determine which genes (out of many thousands) are activated or repressed under two different conditions. J Labels cells that are undergoing apoptosis. 2. What happens when a neutrophin binds it s Trk receptor? Each statement below has two italicized outcomes. Circle the one that correctly describes what happens when a Trk receptor is activated. ½ point each Apoptosis is initiated/inhibited. The Death Induced Signaling Complex (DISC) is formed/not formed. Cytochrome c is retained in/released from the mitochondria. Akt phosphorylates/acetylates target proteins like

7 Name: BIO360 Quiz #7 October 26, points each 1. PI3K phosphorylates phosphatidyl inositol, a membrane phospholipid, producing PIP 3 from PIP 2. What enzyme dephosphorylates PIP 3? 2. The diagram on the right helps to explain what happens during cellular senescence. Label the vertical axis. 3. Wildtype mouse embryo fibroblasts begin to senesce when cultured for many cell divisions. If we begin with mouse embryo fibroblasts that are p53 -/-, will they senesce? Why or why not? 4. ALT relies upon which phenomenon? A. Transcription B. Alternative Splicing C. Kinase Cascade D. Recombination E. Oxidative phosphorylation 5. Using the words below, fill in the two blanks to complete the title of today s paper: inhibits glioma development by targeting Astrocytes EGFR GBM INK4A/ARF MicroRNA-146a Notch1 p53 PTEN

8 Name: BIO360 Quiz #8 November 2, In the paper we discussed, Mei et al. showed that overexpression of mirna-146 could act like a tumor suppressor by affecting both proliferation and differentiation. Fill in the chart below to indicate 1) if mirna-146 increased or decreased each process and 2) one method that was used to measure each process. (1 point each) Increased or Decreased? Method Proliferation Differentiation 2. Fill in the blanks. 1 point each Oxygen is used as a substrate by the enzyme to modify proline amino acids on the transcription factor only when the cell is in conditions. The specific modification is which causes the transcription factor to be bound by pvhl, polyubiquitinated and destroyed. 3. Which class of enzyme directly binds to a substrate protein and transfers a ubiquitin to a lysine side chain of the substrate? (2 points) A. Angiostatin B. Ubiquitin activating enzyme C. Ubiquitin ligase D. 5 1 Integrin E. Ubiquitin conjugating enzyme

9 Name: BIO360 Quiz #9 November 9, point each 1. Cakouros et al. were studying senescence in bone marrow-derived mesenchymal stem cells. We discussed the fact that these cells are multipotent, not totipotent. What s a multipotent stem cell and how is that different from a totipotent one? 2. The ability to invade through the basal lamina is correlated with A. transcriptional silencing of E-Cadherin. B. ankoisis. C. activation of MMPs. D. repression of the transcription factor, Twist. E. changing of which integrins are expressed. 3. Failure to enforce M-phase checkpoints can lead to A. aneuploidy. B. nucleotide alkylation. C. genetic instability. D. translocations. E. proofreading. 4. In cells showing the Warburg effect, which process is dramatically activated? A. Tricarboxylic acid cycle (TCA) B. Oxygen uptake C. Glycolysis D. Oxphos E. mtor-raptor 5. Which one of the following is not a phase of the cancer immunoediting process? A. Equilibrium B. Escape C. Engagement D. Elimination