Cover Letter. Reviewer 1:
|
|
- Cory Horn
- 5 years ago
- Views:
Transcription
1 Cover Letter Michael Yang, M.D., Ph.D. Managing Editor of Cancer Research Frontiers 1188 Willis Ave, #109, Albertson, NY 11507, USA Phone: Dear Dr. Yang, Thank you for your response to our manuscript. We found the comments to be very helpful to improve our content and have made most of the changes requested. We appreciate your consideration of our revised manuscript. Sincerely, Lori Millner, Ph.D., NRCC Reviewer 1: Major concerns: 1. I see a major conflict between the title and the content of the MS. The title does not specifies that the whole analysis was performed on breast cancer cells. This issue can be easily resolved by formulating the title more precisely. More importantly, however, the study analyzes breast cancer cell lines spiked into normal blood. This is handled as a surrogate for circulating tumor cells (CTC) throughout the MS. However, unlike the title suggests, these cells are not equal to CTCs. This issue is a week point of the study. The reviewer makes an excellent point regarding the title of the MS. We have changed the title to better reflect what was actually done in this study. 1. (continued) As a proof of principle, the authors should consider to verify their method on blood samples of breast cancer patients. Based on the fact that CTCs are not detectable in a very high percentage of metastatic breast cancer patients by the conventional EpCam capturing method, the comparative analysis of 2-3 cancer patients might be sufficient to proof the feasibility of the method in detecting non-epithelial CTCs. While we agree that providing patient sample data would further prove the feasibility of the method, the purpose of this manuscript is to introduce a novel method using data from a surrogate system, heterogeneous breast cancer cell lines. Some of the cell lines chosen do not express EpCAM (MDA-MB-231 and subset of SKBR-3) and serve as proof of concept that the method does capture these non-epithelial types of CTCs. This was stated and has been further clarified in the introduction found in lines
2 2. Authors should justify the clinical relevance of the number of breast cancer cells spiked into the blood before isolation (50 into 5 ml). Based on the very rare presence of CTCs in the blood, a sensitivity of 1 CTC per ml blood might be required for clinical application. Authors might want to show sensitivity of the system within this range or bring in data justifying the higher cell numbers they used. The reviewer correctly points out the need for extreme sensitivity. Although the gold standard (CellSearch) considers a positive test to be just 5 or greater cells in 7.5 mls of whole blood for patient samples, it is not ascertainable how many CTCs were actually present. Most spiking experiments use well above this using up to 100 [1], 200 [2], or 300 cells [3]. Additionally, there have been reports of up to hundreds of CTCs per patient sample [4]. 3. Authors should justify the relevance and sufficiency of the selected markers for the identification of breast CTCs, first of all for the positive identification of non-epithelial CTCs. In addition, they should comment on the possible presence of cells with NO positive marker detection (CD45-, EpCam-, HER2-, CD44-, Hoechst+), if they occurred in their analysis and how the method would handle these cells. Non-epithelial cells are retained in our enrichment step because we use a negative depletion method that removes unwanted leukocytes. We used a combination of EpCAM, HER2, and CD44 for positive identification. EpCAM was chosen for identification because it is a commonly used extracellular marker for epithelial CTC detection. In addition to epithelial markers we used 2 additional markers, HER2 and CD44, that are likely to be expressed on more aggressive or non-epithelial CTCs. HER2 was chosen because it s expression has been detected on CTCs in early and metastatic cancer and is not dependent on the primary tumor HER2 status [5, 6]. In several studies, HER2 expression was found more frequently on CTCs than the primary tumor, and expression on CTCs has been associated with poor clinical outcome of early breast cancer [6-8]. CD44 was chosen because it is involved in tumorigenesis, and it s presence on CTCs has been associated with lower overall survival than those with no CD44 expression [9]. CD44 has been described as a marker of tumor-initiating cells and high expression has been observed on a subset of metastasis-initiating CTCs [10, 11]. This explanation has been added in lines to further clarify the use of these markers. Other markers can easily be added to this method but for our feasibility studies, we used only the 3 mentioned. 4. Authors analyzed the heterogeneity of a single cell (SKBR3) line in re-isolated cells by staining for the markers EpCam, HER2 and CD44. Are these obtained percentages in accordance with a FACS analysis of this cell line using the same markers? A FACS analysis has confirmed the presence of SKBR-3 with low EpCAM expression but not the presence of the very rare subpopulation (EpCAM-, HER2-, CD44+) likely because there were so few cells, less than 10 out of 3,000 cells. It is difficult to differentiate these from false positive readings with cytometry. It is easier to positively identify these cells using microscopy because you can visualize the cell and confirm an intact nucleus as well as where the desired marker is expressed.
3 Minor concerns: 1. Abbreviations are not consequently introduced. Authors should check that abbreviations are introduced at first time use. Abbreviations FDA (p2, row48), PBMC (p2, row49), NGS (p3, row70) and WGA (p9, row197) are affected. List might be incomplete. The abbreviations were introduced at their first use as suggested in each line. 2. The authors should consider to reorganize the Introduction part and keep methodology and result description as brief as possible. E.g. last paragraph at p.3 is far too detailed, referring to result tables and figures should be avoided. Some detail in the last paragraph on page 3 lines was removed and added to the methods section (65-66) and the references to figures and tables were removed. To shorten, some detail was removed altogether (80-82). 3. Sentence in row is not clear, what the authors mean. This sentence has been clarified (now in row 92-93). 4. Figure 2 is not clear. According legend figure shows a comparison in the cell capture efficiency of EpCam pos selection vs. negative depletion. In text, the efficiencies for negative depletion are around 80%. What the % Negative on the diagram than stands for? The % negative on the figure refers to the amount of the desired cells (SKBR or MDA) that ended up in the negative (unexpected) fraction. Typically, analysis would only be performed on the positive fraction so we d lose any cells in the negative fraction. This has been clarified in the figure legend and in the text line 241 and There is a mistake in row 240. The marker should be CD44 and not CD45. This has been corrected. Reviewer 2: This is a very interesting process to identify circulating breast cancer tumor cells and agree it is in an interesting way to both identify and be able to deep sequence the cells identified. The technology to use single cell DEPArray to identify CTC is interesting and has not been done much in the literature. However, I do question that the markers selected would actually improve identification of CTCs as still using EPCAM and just adding HER2 and CD44. For particularly breast cancer which is so heterogenous, these markers would not be enough particularly to identify the most aggressive triple negative markers. There are many more EMT markers which could be tested and adding a few more stains may better identify heterogeneous targets. Our justification for the use of these markers of negative outcome and aggression has been clarified under reviewer 1 s comment 3.
4 Would have liked to see a study showing identification of a panel of different breast cancer cell lines and even discussion of looking at dissociated tumor tissue to see if could identify CTCs. This would speak to being able to extend this. We haven t looked at dissociated tumor tissue, but we have identified and differentiated a 4 cell line panel shown below. 4 cell line model MCF-7 HCC SKBR MDA The article however is well written and the technique is very interesting. Would show that it can identify a wider array of breast cancer cell lines or indeed tumor cells and then would be a more complete study. (END) References 1. Xu, L., et al., Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System. PLoS One, (9): p. e Ghazani, A.A., et al., Sensitive and direct detection of circulating tumor cells by multimarker micro-nuclear magnetic resonance. Neoplasia, (5): p Harb, W., et al., Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qpcr Assay. Transl Oncol, (5): p Uenosono, Y., et al., Clinical significance of circulating tumor cells in peripheral blood from patients with gastric cancer. Cancer, (22): p Kallergi, G., et al., Expression of truncated human epidermal growth factor receptor 2 on circulating tumor cells of breast cancer patients. Breast Cancer Res, : p. 113.
5 6. Fehm, T., et al., HER2 status of circulating tumor cells in patients with metastatic breast cancer: a prospective, multicenter trial. Breast Cancer Res Treat, (2): p Wulfing, P., et al., HER2-positive circulating tumor cells indicate poor clinical outcome in stage I to III breast cancer patients. Clin Cancer Res, (6): p Ignatiadis, M., et al., Prognostic value of the molecular detection of circulating tumor cells using a multimarker reverse transcription-pcr assay for cytokeratin 19, mammaglobin A, and HER2 in early breast cancer. Clin Cancer Res, (9): p Ascolani, G., A. Occhipinti, and P. Lio, Modelling circulating tumour cells for personalised survival prediction in metastatic breast cancer. PLoS Comput Biol, (5): p. e Zhang, L., et al., The identification and characterization of breast cancer CTCs competent for brain metastasis. Sci Transl Med, (180): p. 180ra Baccelli, I., et al., Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay. Nat Biotechnol, (6): p
Title: Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles
Author's response to reviews Title: Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles Authors: Julia Tchou (julia.tchou@uphs.upenn.edu) Andrew V Kossenkov (akossenkov@wistar.org)
More informationTitle: Persistent tumor cells in bone marrow of early breast cancer patients after primary surgery are associated with inferior outcome
Author's response to reviews Title: Persistent tumor cells in bone marrow of early breast cancer patients after primary surgery are associated with inferior outcome Authors: Kjersti Tjensvoll (ktje@sus.no)
More informationTitle: MYBBP1A suppresses breast cancer tumorigenesis by enhancing the p53 dependent anoikis
Author's response to reviews Title: MYBBP1A suppresses breast cancer tumorigenesis by enhancing the p53 dependent anoikis Authors: Kensuke Akaogi (kensuke.akaogi@gmail.com) Wakana Ono (wakana315@gmail.com)
More informationA Novel CTC-Detecting Technique Using TelomeScan and Its Clinical Applications
A Novel CTC-Detecting Technique Using TelomeScan and Its Clinical Applications Yasuo Urata CEO and President Oncolys BioPharma Inc. February 16, 2013 Telomere Length is a Limiting Factor for Cell Replication
More informationNature Methods: doi: /nmeth Supplementary Figure 1
Supplementary Figure 1 Finite-element analysis of cell cluster dynamics in different cluster trap architectures. (a) Cluster-Chip (b) Filter (c) A structure identical to the Cluster-Chip except that one
More informationCTC in clinical studies: Latest reports on GI cancers
CTC in clinical studies: Latest reports on GI cancers François-Clément Bidard, MD PhD GI cancers are characterized by Multimodal treatment strategies Treatments are adapted to tumor burden & prognosis
More informationScientific Editing Report
Acknowledge editing support International publication guidelines such as ICMJE guidelines state that all non-author contributions, including editing, should be acknowledged. If you are satisfied with the
More informationHigh Sensitivity Immunomagnetic CTC Isolation as Compared to Alternative Isolation Methods
High Sensitivity Immunomagnetic CTC Isolation as Compared to Alternative Isolation Methods 1. Introduction: An overview of CTC isolation methods 2. Challenges for direct comparisons of CTC recovery 3.
More informationChallenges for use of CTCs as a Diagnostic. Farideh Z. Bischoff, Ph.D. Interim CSO Sr. Director, Translational Clinical Development Biocept, Inc.
Challenges for use of CTCs as a Diagnostic Farideh Z. ischoff, Ph.D. Interim CSO Sr. Director, Translational Clinical Development iocept, Inc. Current Technology for CTC Testing Existing CTC testing platform
More informationTitle: Synuclein Gamma Predicts Poor Clinical Outcome in Colon Cancer with Normal Levels of Carcinoembryonic Antigen
Author's response to reviews Title: Synuclein Gamma Predicts Poor Clinical Outcome in Colon Cancer with Normal Levels of Carcinoembryonic Antigen Authors: Caiyun Liu (liucaiyun23@yahoo.com.cn) Bin Dong
More informationThe CellCollector TM technology
The CellCollector TM technology In vivo isolation of circulating tumor cells by the CellCollector TM system Dr. Klaus Lücke (CEO) Unmet medical need in oncology: Access to tumor cells not only at the time
More informationEarly dissemination in prostate cancer
Early dissemination in prostate cancer Miodrag Guzvic, University of Regensburg, Germany Adjuvant Palliative M0 Initiation Diagnosis Surgery Metastasis Death Intervention window to delay or prevent metastasis
More informationTitle: Prediction of HIV-1 virus-host protein interactions using virus and host sequence motifs
Author's response to reviews Title: Prediction of HIV-1 virus-host protein interactions using virus and host sequence motifs Authors: Perry PE Evans (evansjp@mail.med.upenn.edu) Will WD Dampier (wnd22@drexel.edu)
More informationBelow, we included the point-to-point response to the comments of both reviewers.
To the Editor and Reviewers: We would like to thank the editor and reviewers for careful reading, and constructive suggestions for our manuscript. According to comments from both reviewers, we have comprehensively
More informationTitle:Fulvic acid attenuates homocysteine-induced cyclooxygenase-2 expression in human monocytes
Author's response to reviews Title:Fulvic acid attenuates homocysteine-induced cyclooxygenase-2 expression Authors: Shao-Ju Chien (csjdc@adm.cgmh.org.tw) Te-Chuan Chen (ma4671@gmail.com) Hsing-Chun Kuo
More informationCTC molecular characterization: Are we ready to move forward with clinical testing?
CTC molecular characterization: Are we ready to move forward with clinical testing? Michail Ignatiadis MD, PhD Jules Bordet Institute, Université Libre de Bruxelles Brussels, Belgium Breast cancer: Diagnostics
More informationAuthor's response to reviews
Author's response to reviews Title: Estimation of groin recurrence risk in patients with squamous cell vulvar carcinoma by the assessment of marker gene expression in the lymph nodes. Authors: Magdalena
More informationAuthor's response to reviews
Author's response to reviews Title: Pneumococcal vaccination and otitis media in Australian Aboriginal infants: comparison of two birth cohorts before and after introduction of vaccination Authors: Grant
More informationTitle: Small cell carcinoma arising in Barrett's esophagus: a case report and review of the literature
Author's response to reviews Title: Small cell carcinoma arising in Barrett's esophagus: a case report and review of the literature Authors: Haridimos Markogiannakis (markogiannakis@easy.com) Dimitrios
More informationRE: Title: Practical fecal calprotectin cut-off value for Japanese patients with ulcerative colitis
September 10, 2018 Professor Xue-Jiao Wang, MD Science Editor Editorial Office 'World Journal of Gastroenterology' RE: 40814 Title: Practical fecal calprotectin cut-off value for Japanese patients with
More informationCirculating tumor cells as biomarker for hormonal treatment in breast and prostate cancer. Michal Mego
National Cancer Institute, Slovakia Translational Research Unit Circulating tumor cells as biomarker for hormonal treatment in breast and prostate cancer Michal Mego 2 nd Department of Oncology, Faculty
More informationThe Presence and Persistence of Resistant and Stem Cell- Like Tumor Cells as a Major Problem in Ovarian Cancer
Welcome! The Presence and Persistence of Resistant and Stem Cell- Like Tumor Cells as a Major Problem in Ovarian Cancer Prof. Sabine Kasimir-Bauer Department of Gynecology and Obstetrics University Hospital
More information- 1 - Reviewers' comments: Reviewer #1 (Remarks to the Author):
Reviewers' comments: Reviewer #1 (Remarks to the Author): Summary: In this manuscript, the authors characterized cellular and transcriptional heterogeneity of breast cancer using single-cell RNA sequencing.
More informationDNA Methylation of Tumor Suppressor and Metastasis Suppressor Genes in Circulating Tumor Cells and corresponding Circulating Tumor DNA
DNA Methylation of Tumor Suppressor and Metastasis Suppressor Genes in Circulating Tumor Cells and corresponding Circulating Tumor DNA Maria Chimonidou 1, Areti Strati 1, Nikos Malamos 2, Vasilis Georgoulias
More informationDear Dr. Villanueva,
22-12-2017 Dear Dr. Villanueva, We would like to thank you for your interest in our paper and the opportunity to resubmit our manuscript Living network meta-analysis for reducing research waste: an empirical
More informationTitle: Prevalence of sexual, physical and emotional abuse in the Norwegian Mother and Child Cohort Study
Author's response to reviews Title: Prevalence of sexual, physical and emotional abuse in the Norwegian Mother and Child Cohort Study Authors: Marie F Sorbo (marie.flem.sorbo@ntnu.no) Hilde Grimstad (hilde.grimstad@ntnu.no)
More informationSupplementary Figures
Supplementary Figures Supplementary Figure 1 DOT1L regulates the expression of epithelial and mesenchymal markers. (a) The expression levels and cellular localizations of EMT markers were confirmed by
More informationComprehensive isolation, identification, and nucleic acid analysis of single breast cancer cells: CTC-isoTECH
Comprehensive isolation, identification, and nucleic acid analysis of single breast cancer cells: CTC-isoTECH Lori M. Millner 1,2*, Lindsay N. Strotman 1,2, Kevin S. Goudy 1,2, Mark W. Linder 1,2, Roland
More informationAuthor's response to reviews
Author's response to reviews Title: Intensive post-operative follow-up of breast cancer patients with tumour markers: CEA, TPA or CA15.3 vs MCA and MCA-CA15.3 vs CEA-TPA-CA15.3 panel in the early detection
More informationSkin squamous cell carcinoma propagating cells increase with tumour progression and invasiveness
Manuscript EMBO-2012-83706 Skin squamous cell carcinoma propagating cells increase with tumour progression and invasiveness Gaëlle Lapouge, Benjamin Beck, Dany Nassar, Christine Dubois, Sophie Dekoninck
More informationDisclosure. Summary. Circulating DNA and NGS technology 3/27/2017. Disclosure of Relevant Financial Relationships. JS Reis-Filho, MD, PhD, FRCPath
Circulating DNA and NGS technology JS Reis-Filho, MD, PhD, FRCPath Director of Experimental Pathology, Department of Pathology Affiliate Member, Human Oncology and Pathogenesis Program Disclosure of Relevant
More informationQuantification of early stage lesions for loss of p53 should be shown in the main figures.
Reviewer #1 (Remarks to the Author): Expert in prostate cancer The manuscript "Clonal dynamics following p53 loss of heterozygosity in Kras-driven cancers" uses a number of novel genetically engineered
More informationTitle: Oral administration of PPC enhances antigen-specific CD8+ T cell responses while reducing IgE levels in sensitized mice.
Author's response to reviews Title: Oral administration of PPC enhances antigen-specific CD8+ T cell responses while reducing IgE levels in sensitized mice. Authors: Mike Burrows (mburrows@tampabayresearch.org)
More informationCirculating Tumor Cells in non- Metastatic Triple Negative Breast Cancer
Circulating Tumor Cells in non- Metastatic Triple Negative Breast Cancer Carolyn Hall, Ph.D. Department of Surgical Oncology The University of Texas MD Anderson Cancer Center Triple Negative Breast Cancer
More informationTitle: Co-morbidities, complications and causes of death among people with femoral neck fracture - A three-year follow-up study.
Author s response to reviews Title: Co-morbidities, complications and causes of death among people with femoral neck fracture - A three-year follow-up study. Authors: Monica Berggren (monica.langstrom@umu.se)
More informationBulfoni et al. Breast Cancer Research (2016) 18:30 DOI /s
Bulfoni et al. Breast Cancer Research (2016) 18:30 DOI 10.1186/s13058-016-0687-3 RESEARCH ARTICLE In patients with metastatic breast cancer the identification of circulating tumor cells in epithelial-to-mesenchymal
More informationCirculating Tumor Cells (CTC) Technologies
Table of Contents MIR036 I. SCOPE AND METHODOLOGY Scope of the Study Analytics and data presented in this report pertain to several parameters such as - Research Methodology This report is uniquely researched
More informationCochrane Breast Cancer Group
Cochrane Breast Cancer Group Version and date: V3.2, September 2013 Intervention Cochrane Protocol checklist for authors This checklist is designed to help you (the authors) complete your Cochrane Protocol.
More informationAuthor s response to reviews
Author s response to reviews Title: Prevalence of chronic hepatitis B virus infection and infrastructure for its diagnosis in Madagascar: implication for the WHO's elimination strategy Authors: Soa Fy
More informationAuthor's response to reviews
Author's response to reviews Title: Diabetes duration and health-related quality of life in individuals with onset of diabetes in the age group 15-34 years - a Swedish population-based study using EQ-5D
More informationPage 4. Line 7 and 8. Do these stats refer to children worldwide? Please clarify.
Reviewer s report Title: Management of severe acute malnutrition by cow milk in resource contraints settings: experience of the Nutritional Centre of the University Clinics of Graben Version: 0 Date: 27
More informationAuthor's response to reviews
Author's response to reviews Title:microRNA alterations in mammary epithelial stem cells: a crucial contributing factor towards breast cancer risk reduction in case of early pregnancy Authors: Sushmita
More informationCellecta Overview. Started Operations in 2007 Headquarters: Mountain View, CA
Cellecta Overview Started Operations in 2007 Headquarters: Mountain View, CA Focus: Development of flexible, scalable, and broadly parallel genetic screening assays to expedite the discovery and characterization
More informationCell-free tumor DNA for cancer monitoring
Learning objectives Cell-free tumor DNA for cancer monitoring Christina Lockwood, PhD, DABCC, DABMGG Department of Laboratory Medicine 1. Define circulating, cell-free tumor DNA (ctdna) 2. Understand the
More informationThese comments are an attempt to summarise the discussions at the manuscript meeting. They are not an exact transcript.
Dear dr. Weber, We would like to thank you for the review of our manuscript entitled Cervical screening with an interval beyond five years requires different rescreen times for HPV-negative and HPVpositive,
More informationLa biopsia liquida. Aldo Scarpa. Anatomia Patologica e ARC-NET Centro di Ricerca Applicata sul Cancro
La biopsia liquida Aldo Scarpa Anatomia Patologica e ARC-NET Centro di Ricerca Applicata sul Cancro Azienda Ospedaliera Universitaria Integrata di Verona Obstacles to precision oncology Genomic heterogeneity
More informationUNIVERSITY OF CALIFORNIA, LOS ANGELES
UNIVERSITY OF CALIFORNIA, LOS ANGELES BERKELEY DAVIS IRVINE LOS ANGELES MERCED RIVERSIDE SAN DIEGO SAN FRANCISCO UCLA SANTA BARBARA SANTA CRUZ DEPARTMENT OF EPIDEMIOLOGY SCHOOL OF PUBLIC HEALTH CAMPUS
More informationAmare Nigatu
Author s response to reviews Title: Self-reported acute pesticide intoxication in Ethiopia. Authors: Amare Nigatu (Amare.Nigatu@uib.no;Amare.Nigatu@igs.uib.no) Magne Bråtveit (Magne.Bratveit@uib.no) Bente
More informationTitle: Peritumoral Vascular Invasion and NHERF1 expression define an immunophenotype of grade 2 invasive breast cancer associated with poor prognosis
Author's response to reviews Title: Peritumoral Vascular Invasion and NHERF1 expression define an immunophenotype of grade 2 invasive breast cancer associated with poor prognosis Authors: Andrea Malfettone
More informationTitle: Treatment adherence among sputum smear-positive pulmonary tuberculosis patients in mountainous areas in China
Author's response to reviews Title: Treatment adherence among sputum smear-positive pulmonary tuberculosis patients in mountainous areas in China Authors: Song Yao (ys506506@yahoo.com.cn) Wen-Hui Huang
More informationAuthor s response to reviews
Author s response to reviews Title: Association between Charlson comorbidity index score and outcome in patients with stage IIIB-IV non-small cell lung cancer Authors: Lei Zhao (zhaoleilei0507@126.com)
More informationSupplemental Table S1
Supplemental Table S. Tumorigenicity and metastatic potential of 44SQ cell subpopulations a Tumorigenicity b Average tumor volume (mm ) c Lung metastasis d CD high /4 8. 8/ CD low /4 6./ a Mice were injected
More informationIntegrated platform for liquid biopsy-based personalized cancer medicine
Integrated platform for liquid biopsy-based personalized cancer medicine Dr. Bernhard Polzer Fraunhofer ITEM-Regensburg Personalized Tumor Therapy Personalized cancer therapy Primary tumor single tumor
More informationYoungnam Cho. National Cancer Center Biomarker Branch
Youngnam Cho National Cancer Center Biomarker Branch Contents 1. Liquid Biopsy 2. Circulating Tumor Cells from Blood 3. Cell-free DNA from Blood 1. Liquid biopsy Cancer Diagnosis IMAGING TISSUE BIOPSY
More informationAuthor's response to reviews
Author's response to reviews Title: Measles transmission from an anthroposophic community to the general population, Germany 2008 - Effect of early intervention on size and duration of measles clusters
More informationInvestigator Initiated Study Proposal Form
Please submit completed form to IISReview@KCI1.com Date Submitted Name & Title Institution Address Phone Number Email Address Principal Investigator / Institution YES NO Multi Center Study Acelity Product(s)
More informationAdnaNews. News from the meeting on Advances in Circulating Tumor Cells (ACTC), Reythymnon, Crete, Greece October 8-11, 2014.
News from the meeting on Advances in Circulating Tumor Cells (ACTC), Reythymnon, Crete, Greece October 8-11, 2014. The ACTC meeting (www.actc2014.org) is together with the ISMRC meeting one of the most
More informationTitle: Non-small cell lung carcinoma in an adolescent, manifested by acute paraplegia due to spinal metastases - case report
Author's response to reviews Title: Non-small cell lung carcinoma in an adolescent, manifested by acute paraplegia due to spinal metastases - case report Authors: Ulrike Ackert (ulrike_ackert@hotmail.com)
More informationAuthor's response to reviews
Author's response to reviews Title: Corticosteroid co-treatment induces resistance to chemotherapy in surgical resections, xenografts, and established cell lines of pancreatic cancer Authors: Chengwen
More informationTitle: Quality of life in childhood epilepsy with lateralized focus
Author's response to reviews Title: Quality of life in childhood epilepsy with lateralized focus Authors: Krystyna A. Mathiak (krystyna.mathiak@psych.uw.edu.pl) Malgorzata Luba (malgosia.luba@gmail.com)
More informationTitle: Nuclear entrapment and extracellular depletion of PCOLCE is associated with muscle degeneration in oculopharyngeal muscular dystrophy
Author's response to reviews Title: Nuclear entrapment and extracellular depletion of PCOLCE is associated with muscle degeneration in oculopharyngeal muscular dystrophy Authors: Vered Raz (v.raz@lumc.nl)
More informationTitle: Prevalence and incidence of multiple sclerosis in central Poland,
Author s response to reviews Title: Prevalence and incidence of multiple sclerosis in central Poland, 2010-2014 Authors: Waldemar Brola (wbrola@wp.pl) Piotr Sobolewski (piotrsobolewski@poczta.onet.pl)
More informationMatrix metalloproteinase 1 and circulating tumor cells in early breast cancer.
National Cancer Institute Slovakia Translational Research Unit Slovakia Matrix metalloproteinase 1 and circulating tumor cells in early breast cancer. Mego M 1, 2, Karaba M 2, Cierna Z 1, Janega P 1, Minarik
More informationPEER REVIEW HISTORY ARTICLE DETAILS TITLE (PROVISIONAL)
PEER REVIEW HISTORY BMJ Open publishes all reviews undertaken for accepted manuscripts. Reviewers are asked to complete a checklist review form (http://bmjopen.bmj.com/site/about/resources/checklist.pdf)
More informationTitle:Video-confidence: a qualitative exploration of videoconferencing for psychiatric emergencies
Author's response to reviews Title:Video-confidence: a qualitative exploration of videoconferencing for psychiatric emergencies Authors: Marianne V Trondsen (marianne.trondsen@telemed.no) Stein Roald Bolle
More informationTitle: Effects of short-term heart rate variability biofeedback on long-term abstinence in alcohol dependent patients - a one-year follow up
Author s response to reviews Title: Effects of short-term heart rate variability biofeedback on long-term abstinence in alcohol dependent patients - a one-year follow up Authors: Ana Isabel Penzlin (ana.penzlin@ppcr.org)
More informationSSM signature genes are highly expressed in residual scar tissues after preoperative radiotherapy of rectal cancer.
Supplementary Figure 1 SSM signature genes are highly expressed in residual scar tissues after preoperative radiotherapy of rectal cancer. Scatter plots comparing expression profiles of matched pretreatment
More informationTitle:DNA Methylation Subgroups and the CpG Island Methylator Phenotype in Gastric Cancer: A Comprehensive Profiling Approach
Author's response to reviews Title:DNA Methylation Subgroups and the CpG Island Methylator Phenotype in Gastric Cancer: A Comprehensive Profiling Approach Authors: Marie Loh (m.loh@imperial.ac.uk) Natalia
More informationTitle: Cystatin E/M suppresses legumain activity and invasion of human melanoma
Author's response to reviews Title: Cystatin E/M suppresses legumain activity and invasion of human melanoma Authors: Jon J Briggs JJB (jbriggs33@yahoo.com) Mads H Haugen MHH (Mads.Haugen@rr-research.no)
More informationTitle: Protocol-based management of older adults with hip fractures in Delhi, India: a feasibility study
Reviewer s report Title: Protocol-based management of older adults with hip fractures in Delhi, India: a feasibility study Version: 0 Date: 16 Nov 2015 Reviewer: Cristin Ryan Reviewer's report: This paper
More informationTitle: The effect of Breast Cancer Awareness Month on Internet search activity - a comparison with awareness campaigns for lung and prostate cancer
Author's response to reviews Title: The effect of Breast Cancer Awareness Month on Internet search activity - a comparison with awareness campaigns for lung and prostate cancer Authors: Ronan W Glynn (ronanglynn@doctors.net.uk)
More informationSurvey Results Q1. How would you best describe your organization?
Survey Results Q1. How would you best describe your organization? Q2. How high would you rate the priority of Circulating Tumor Cells in you organization? Q3. What do you think is the biggest challenge
More informationSeptember 23, The Role of In Vitro Diagnostic Tests in Pediatric Master Protocol Development
The Role of In Vitro Diagnostic Tests in Pediatric Master Protocol Development September 23, 2016 Anand Pathak, MD, PhD, MPH Medical Officer Molecular Genetics Branch Division of Molecular Genetics and
More informationAuthor s response to reviews:
Author s response to reviews Title: Is Zimbabwe ready to transition from anonymous unlinked sero-surveillance to using Prevention of Mother to Child Transmission of HIV (PMTCT) program data for HIV Surveillance?:
More informationITERATIVELY TRAINING CLASSIFIERS FOR CIRCULATING TUMOR CELL DETECTION
ITERATIVELY TRAINING CLASSIFIERS FOR CIRCULATING TUMOR CELL DETECTION Yunxiang Mao 1, Zhaozheng Yin 1, Joseph M. Schober 2 1 Missouri University of Science and Technology 2 Southern Illinois University
More informationTITLE: Selective Oncolytic Therapy for Hypoxic Breast Cancer Cells. CONTRACTING ORGANIZATION: Ordway Research Institute Albany, New York 12208
AD Award Number: W81XWH-06-1-0586 TITLE: Selective Oncolytic Therapy for Hypoxic Breast Cancer Cells PRINCIPAL INVESTIGATOR: Michael T. Fasullo, Ph.D. CONTRACTING ORGANIZATION: Ordway Research Institute
More informationAuthor's response to reviews
Author's response to reviews Title: Wnt1 is epistatic to Id2 in modeling mammary gland development and in causing mammary tumors Authors: Susan Marino (smarino@cmgm.stanford.edu) Claire Romelfanger (clairefrog@yahoo.com)
More informationTechnology. Depletion. Bat-El Tayar. Rony Basilaa. Pazit Enker-Ohana. Ofer Klein. 13, 2015 /jab.v3n2.7081
Depletion of CD45 as Pre-Enrichment Stepp for Rare Cells Detection Using a Novel Immunomagnetic Technology Abuelhija Mahmoud, Ph.D (Corresponding author) Biological Research Department, Ziv Medical Center
More informationAuthor's response to reviews
Author's response to reviews Title: Diagnostic accuracy of point-of-care testing for acute coronary syndromes, heart failure and thromboembolic events in primary care: a cluster-randomised controlled trial
More informationManuscript ID BMJ R1 entitled "Education and coronary heart disease: a Mendelian randomization study"
BMJ - Decision on Manuscript ID BMJ.2017.03 7504.R1 Body: 11-May-2017 Dear Dr. Tillmann Manuscript ID BMJ.2017.037504.R1 entitled "Education and coronary heart disease: a Mendelian randomization study"
More informationIdentifying genomic signatures in circulating breast tumour cells
Identifying genomic signatures in circulating breast tumour cells 9 th ISMRC 2013, Paris, France September 25th, 2013 NISHA KANWAR PhD Candidate Department of Laboratory Medicine and Pathobiology University
More informationSUPPLEMENTARY INFORMATION
doi:.38/nature8975 SUPPLEMENTAL TEXT Unique association of HOTAIR with patient outcome To determine whether the expression of other HOX lincrnas in addition to HOTAIR can predict patient outcome, we measured
More informationTips on Successful Writing and Getting Published Rita F. Redberg, MD, MSc, FACC, FAHA Professor of Medicine Editor, JAMA Internal Medicine
Tips on Successful Writing and Getting Published Rita F. Redberg, MD, MSc, FACC, FAHA Professor of Medicine Editor, JAMA Internal Medicine Rita F. Redberg, MD, MSc and 1 Helpful Hints!!! Correct journal
More informationDetection of the Circulating Tumor Cells in Cancer Patients
Detection of the Circulating Tumor Cells in Cancer Patients Athanasios Armakolas; Zacharoula Panteleakou; Adrianos Nezos; Aikaterini Tsouma; Maria Skondra; Peter Lembessis; Nikolaos Pissimissis; Michael
More information논문투고및투고후소통하기 : 영문교정작업, 실제논문투고하기, revision 답변달기, query form 작성하기
Apr 28, 2018 9:20-9:40 JGO Workshop 논문투고및투고후소통하기 : 영문교정작업, 실제논문투고하기, revision 답변달기, query form 작성하기 연세의대이정윤 좋은아이디어를얻기위하여, 타인으로부터유익한암시를받을때가있음. 새로운아이디어는두사람이상의지식및아이디어를함께모을때생겨나는경우가있음. 토론은잘못의발견에유익한방법임. 혼자독립하여동료와이야기를나누지않는연구자는틀린길을걸어가다많은시간을낭비하게되는경우가있음.
More informationAward Number: W81XWH TITLE: Characterizing an EMT Signature in Breast Cancer. PRINCIPAL INVESTIGATOR: Melanie C.
AD Award Number: W81XWH-08-1-0306 TITLE: Characterizing an EMT Signature in Breast Cancer PRINCIPAL INVESTIGATOR: Melanie C. Bocanegra CONTRACTING ORGANIZATION: Leland Stanford Junior University Stanford,
More informationAssessment performed on Tuesday, July 29, 2014, at Lions Gate Hospital, North Vancouver
Assessors report for ciqc Run 37: BRAF V600E (April 2014) Assessors: B Gilks, R Wolber, K Ung, P Tavassoli, J Garratt and J Won (recorder) Assessment performed on Tuesday, July 29, 2014, at Lions Gate
More informationAuthor's response to reviews
Author's response to reviews Title:Therapeutic effect of intravesical administration of paclitaxel solubilized with poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) in an orthotopic
More informationIntroduction. Structure
SOPs/Instructions for Authors and templates for standard ESMO Clinical Practice Guidelines (CPGs) and ESMO-MCBS Scores ESMO Guidelines Committee, October 2015, latest revision April 2018 Introduction The
More informationReviewers' comments: Reviewer #1 (Remarks to the Author):
Reviewers' comments: Reviewer #1 (Remarks to the Author): In this study the authors analysed 18 deep penetrating nevi for oncogenic genomic changes (single nucleotide variations, insertions/deletions,
More informationTitle: Intention-to-treat and transparency of related practices in randomized, controlled trials of anti-infectives
Author s response to reviews Title: Intention-to-treat and transparency of related practices in randomized, controlled trials of anti-infectives Authors: Robert Beckett (rdbeckett@manchester.edu) Kathryn
More informationReviewers' comments: Reviewer #1 (Remarks to the Author):
Reviewers' comments: Reviewer #1 (Remarks to the Author): In the manuscript Rational Combination of CXCL11-Expressing Oncolytic Virus and PD-L1 Blockade Works Synergistically to Enhance Therapeutic Efficacy
More informationTitle:Spousal diabetes as a diabetes risk factor: A systematic review and meta-analysis
Author's response to reviews Title:Spousal diabetes as a diabetes risk factor: A systematic review and meta-analysis Authors: Aaron Leong (aaron.leong@mail.mcgill.ca) Elham Rahme (elham.rahme@mcgill.ca)
More informationCancers of unknown primary : Knowing the unknown. Prof. Ahmed Hossain Professor of Medicine SSMC
Cancers of unknown primary : Knowing the unknown Prof. Ahmed Hossain Professor of Medicine SSMC Definition Cancers of unknown primary site (CUPs) Represent a heterogeneous group of metastatic tumours,
More informationAmyotrophic Lateral Sclerosis: Developing Drugs for Treatment Guidance for Industry
Amyotrophic Lateral Sclerosis: Developing Drugs for Treatment Guidance for Industry DRAFT GUIDANCE This guidance document is being distributed for comment purposes only. Comments and suggestions regarding
More informationPEER REVIEW HISTORY ARTICLE DETAILS VERSION 1 - REVIEW
PEER REVIEW HISTORY BMJ Paediatrics Open publishes all reviews undertaken for accepted manuscripts. Reviewers are asked to complete a checklist review form and are provided with free text boxes to elaborate
More informationTitle: Home Exposure to Arabian Incense (Bakhour) and Asthma Symptoms in Children: A Community Survey in Two Regions in Oman
Author's response to reviews Title: Home Exposure to Arabian Incense (Bakhour) and Asthma Symptoms in Children: A Community Survey in Two Regions in Oman Authors: Omar A Al-Rawas (orawas@squ.edu.om) Abdullah
More informationTitle: A Prospective Study of Dietary Selenium Intake and Risk of Type 2 Diabetes
Author's response to reviews Title: A Prospective Study of Dietary Selenium Intake and Risk of Type 2 Diabetes Authors: Saverio Stranges (S.Stranges@warwick.ac.uk) Sabina Sieri (Sabina.Sieri@istitutotumori.mi.it)
More informationTitle: Socioeconomic conditions and number of pain sites in women
Author's response to reviews Title: Socioeconomic conditions and number of pain sites in women Authors: Finn E Skjeldestad (fisk@fhi.no) Toril Rannestad (Toril.Rannestad@hist.no) Version: 2 Date: 17 January
More information2. Could you insert a reference, proving your statement on p. 5, l. 66/67?
Author s response to reviews Title: Representative survey on idiopathic environmental intolerance attributed to electromagnetic fields in Taiwan and comparison with the international literature Authors:
More information