In vivo prediction of anti-tumor effect of 3- bromopyruvate in Hepatocellular Carcinoma using Tc-99m labeled annexin-v imaging

Transcription

1 In vivo prediction of anti-tumor effect of 3- bromopyruvate in Hepatocellular Carcinoma using Tc-99m labeled annexin-v imaging Won Kim, Jung-Hwan Yoon*, Gi Jeong Cheon, Tae Sup Lee, Chung Yong Kim Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea

2 BACKGROUND intracellular energy generation mechanism normoxic condition O 2 dependent mitochondrial ATP generating system Normal cell salvage pathway hypoxic condition glycolytic system Hexokinase II Cancer cell HCC hypoxia HIF-1α

3 Hexokinase II inhibitor as a new chemotherapeutic agent in HCC tumor cell glucose 3-BP glucose-6-p glucose F-6-P glucose ATP ATP FDP HK Br O - C H 2 O O pyruvate ADP+Pi ATP 3-Bromopyruvate lactate lactate Geschwind JF, et al.,cancer RESEARCH 62 (2002)

4 Hypoxia stimulates HCC cell growth through Hexokinase II induction. Huh-BAT % change of optical densities Huh-BAT day Hypoxia Normoxia Hypoxia HIF-1α hexokinase I hexokinase II mcl-1 HSP70 actin Yoon JH, et al.,journal of Hepatology 42 (2005) 120KD 100KD 100KD 40KD 70KD 43KD

5 HK II inhibitor suppresses celluar growth in hypoxic condition more than normoxic condition by inducing apoptotic cell death. % change of optical densities Huh-BAT Hypoxia Normoxia 3-BP um % change of optical densities Necrosis Apoptosis hr DAPI stain Yoon JH, et al.,journal of Hepatology 42 (2005)

6 HK II inhibitor induces apoptotic cell death through activation of mitochondrial apoptotic signaling cascades. 3-BP min capsase 8 bid Huh-BAT, cytosol 40KD 24KD cytochrome c 10 15KD 0 3-BP AIF Smac/DIABLO capsase 3 actin Yoon JH, et al.,journal of Hepatology 42 (2005) 67KD 22KD 17KD 43KD Confocal microscopy, Nuclear translocation of AIF min

7 Death Receptor FADD DISC 3-BP Caspase 8/10 Bid t-bid Mitochondria Apoptosis Apaf-1 Procaspase 9 Cytochrome c Caspase 9 Effector Caspases

8 Aim (1) To analyze which mitochnodrial apoptotic signaling is activated by HK II inhibitor, specifically focused on PTPC Hypothesis OM PBR VDAC HK CK 3-BP OM cytochrome c PBR 3-BP VDAC CK HK IM ANT CyD IM ANT CyD Permeability Transition Pore Complex (PTPC)

9 Which mitochondrial apoptotic signaling is activated by HK II inhibitor, focused on PTPC? Process Method Huh-BAT + 3-BP Immunoprecipitation of PTPC using VDAC Ab Mitochondrial extract Hexokinase II Immunoblot for hexokinase II

10 Aim (2) To prove anti-tumor effect of HK II inhibitor in in vivo apoptosis model of by using Tc-99m-HYNIC Annexin V imaging

11 Anti-tumor effect of 3-BP in in vivo model of HCC Process Method Day 0 C3H Mouse hepatoma model MH134 cells (2.5x10 5 /0.1mL i.d. to flank) 3-BP i.p. for 5 days Control 3-BP 5mg/kg 3-BP 10mg/kg Day 14 Comparison of tumor volumes TUNEL staining Tc-99m-HYNIC Annexin V scan

12 Results Immunoprecipitation of PTPC using anti-sera for VDAC & Immunoblot for hexokinase II cell lysate 3-BP cell lysate 3-BP 113.5KD 97KD 96KD HKII 113.5KD 97KD 96KD Hexokinase II (100KD)

13 Pretreatment Posttreatment Control 3-BP 10mg/kg

14 The change of TV in control group mean tumor volume control 3BP 5mg/kg 3BP 10mg/kg tumor volume before after pretreatment tumor volume posttretment tumor volume The change of TV in 3-BP 5mg/kg The change of TV in 3-BP 10mg/kg tumor volume tumor volume pretreatment tumor volume posttretment tumor volume pretreatment tumor volume posttretment tumor volume

15 Control 3-BP 10mg/kg H&E TUNEL

16 Apoptotic index according to 3-BP concentration 3.00 control 3BP5 3BP10 P<0.05

17 99m Tc-HYNIC-Annexin V image in 3-BP treatment Con 1 Con 2 Tx1d Tx3d 99m Tc-HYNIC-Annexin V (50 Ci/ 2 g/ 0.1 ml/ head) injected into tail vein of mice Gamma camera images were obtained at 1 h post injection

18 Tumor-to backgroud uptake ratio (UR) Con 1 Con 2 Tx1d Tx3d Tumor Volume(mm 3 ) Con 1 Con 2 Tx1d Tx3d Region-of-interest(ROI) count T Con 1 Con 2 Tx1d Tx3d Counterpart thigh muscle

19 Summary Hexokinase II inhibitor induced the activation of mitochondrial apoptotic signaling cascades by dissociating HK II from PTPC. Apoptosis-inducing anti-tumor effect of 3-BP was demonstrated in in vivo model of HCC by apoptotic imaging using Tc-99m-HYNIC annexin V.