14.15 Lecture Protein-bound lipid peroxidation products: structures and functions K. Uchida (Japan)

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1 PROGRAM FRIDAY 16TH JUNE Opening Remarks U.M. Marinari, G. Poli Session I LIPID PEROXIDATION PRODUCTS AND ANALYTICAL PROCEDURES Chairmen: P. Eckl, C. Spickett Protein-bound lipid peroxidation products: structures and functions K. Uchida (Japan) Mass spectrometric strategies for identification of peptides and proteins modified by alfa, beta-unsaturated aldehydes G. Aldini (Italy) ) Structural characterization of protein adducts generated during non-enzymatic oxidation of linoleic acid L. Sayre 2) Markers of lipid peroxidation in plasma of rats treated with ferric-nitrilotriacetate, in relation to kidney and liver modifications G. Corona 3) Enzyme-linked immunosorbent assay for 4-hydroxynonenal-histidine conjugates S. Borovic 4) Synthesis and characterization of 4-hydroxinonenal (HNE) derivates A. Repetto Coffee break Session II BIOLOGICAL TARGETS OF HNE Chairmen: A. Pompella, Juan Vina 16.45

2 Hepatocellular ProteinTargets of 4-HNE Modification D. Petersen (USA) ) HNE-modified proteins: formation, distribution and fate T. Grune 2) Comparison of the yelds of 4-hydroxy-2-nonenal adduct to glutathione and to DNA in human cells O. Falletti 3) Differential sensivity of astrocytes and cerebral endothelial cells towards oxidative stress H. Jaksch 4) Oral Communication N. Traverso Posters SATURDAY 17TH JUNE 2006 Session III METABOLISM AND DETOXIFICATION Chairmen: T. Grune, J. Schaur Role of HNE and its metabolites in signalling Y. Awasthi (USA) ) Analysis of HNE and its metabolities: Chiral and LC-ESI-MS applications M. Picklo 2) Higher circulating 4-hydroxinonenal-protein thioether adducts (4HNE-P) correlate with more severe diastolic dysfunction in Spontaneously Hypertensive Rats C. Asselin 3) HNE produced by malarial pigment hemozoin and parasitized RBCs generates HNEprotein adducts and decrease RBC deformability O. Skorokhod 2

3 4) Trans-4-hydroxy-2-nonenal inhibits transcription in mammalian cells and activates transcription-coupled repair pathway L. Maddakuri Coffee break Session IV SIGNAL TRANSDUCTION AND GENE EXPRESSION Chairmen:E. Albano, H.J. Forman Resveratrol protects against 4-hydroxynonenal-induced apoptosis by blocking JNK and c- UN/AP-1 signaling H. Basaga (Turkey) ) Import and signaling of oxidized phospholipids in vascular smooth muscle cells A. Hermetter 2) Oxidized phospholipids modulate inflammatory processes relevant to atherosclerosis C. Erridge 3) Up-regulation of TGFbeta1 by 9-oxononanoyl-cholesterol in U937 cells P. Gamba 4) HNE activates the EpREs in glutamate cysteine ligase subunits through activation of Nrf2 and c-jun in human bronchial epithelial cells. H.J. Forman Lunch & Posters Session V CARCINOGENESIS AND TUMOR GROWTH Chairmen: M. U. Dianzani, N. Zarkovic Lipid peroxide mediated DNA damage in colonic stem cells. J. Lunec ) Both ω-3 and ω-6 polynsaturated fatty acids decrease growth of the A549 human lung 3

4 tumor cells through lipid peroxidation induction R. A. Canuto 2) Mechanisms of telomerase activity and htert expression inhibition by HNE in human leukemic cells S. Pizzimenti 3) DHN-MA, the major unrinary metabolite of HNE is a new marker of colon cancer risk associated with heme intake F. Gueraud 4) Histological distribution of acrolein-2 deoxyadenosine in humane colon adenomas, carcinomas and non-malignant colon epithelium. K. Zarkovic Coffee break & Posters Hermann Esterbauer AWARD Gala Dinner SUNDAY 18TH JUNE 2006 Session VI PATHOPHYSIOLOGY Chairmen: M. Comporti, Josè Vina Transcriptional activation of antioxidant stress genes in vascular cells G. Mann (UK) ) Genomic and proteinomic studies in mammary gland during the lactation/weaning cycle. Role of oxidative stress J. Vina 2) Mitochondria peroxide synthesis and CPT-1 nitrosylation during nash development: a model of oxidative stress diseases. G. Serviddio 3) Hydroxynonenal and oxidized LDL modulate platelet-derived growth factor receptor- 4

5 {beta} in vascular cells and atherosclerotic lesions. A. Negre - Salvayre 4) Occurrence of isoprostane receptors activating fibrogenic effects on hepatic stellate cells M. Comporti 5) Oxidized phospholipids in apoptotic cells as possible trigger for anti-phospholipid antibody formation in chronic liver disease M. Vidali 6) Oxidative stress is involved in the development of cyctic fibrosis-related diabetes (CFRD) T. Ntimbane Closing Remarks Farewell Reception 5