ANALYTICAL SCALE AGILENT LCMS SYSTEMS OPERATIONAL QUALIFICATION

Transcription

1 ANALYTICAL SCALE AGILENT LCMS SYSTEMS OPERATIONAL QUALIFICATION Overview This document describes the test program for qualifying Agilent LCMS systems. The following tables list all MS source-specific OQ tests. Source abbreviations used in this document include: AJST: CI: DSAJST: DSCI: DSES: ES: MM: MM(CI): MM(EI): PI: AP-ESI with Agilent Jet Stream Technology AP-CI Dual spray AJST Dual spray AP-CI Dual spray API-ES API-ES Multimode API-ES and AP-CI modes Multimode AP-CI mode only Multimode API-ES mode only AP-PI Key: Fixed HPLC setpoints/limits Variance allowed Single Quadrupole: Standard QQ Test Suite Test Setpoints and Parameters Limits Model(s) and/or Source(s) Vacuum Verification N/A High vacuum 2E-6 torr and 2E-5 torr (pos. and neg. modes) Response Linearity Model-specific; see Appendix: Masses for section Injection volume on column: 5 l* Evaluated mass is 311 m/z All used masses ± 0.2 m/z Coeff. of determination (r2) Injection Precision Area RSD 10.00% Height RSD 10.00% Injection Carry Over Area carry over 1.00% Height carry over 1.00% Signal to Noise Signal to noise 400 Signal to noise 20 Signal to noise 100 * 1 ul for 6150B Series w/ AJST AJST, CI, ES, MM, PI G6150A w/ajst CI, MM(CI) ESI, MM(ES), PI Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 1 of 10

2 Triple Quadrupole: Standard OQ Test Suite Test Setpoints and Parameters Limits Model(s) and/or Source(s) Vacuum Verification N/A 2.7E-5 torr/3.4e-5 torr 1.8E-5 torr/2.7e-5 torr 2.5E-5 torr / 3.5E-5 torr 1.5E-5 torr/2.7e-5 torr 4.45E-5 torr / 6.0E-5 torr 2.7E-5 torr / 4.2E-5 torr (pos. and neg. modes, Q1 and Q3) Model-specific; see Appendix: Masses for section Used masses ± 0.2 m/z Response Linearity Injection volume on column: 5 l* Coeff. of det. (r2) Injection Precision Evaluated mass is 156 m/z Area RSD 10.00% Injection Precision (Negative Mode; optional test, extra fee) Area RSD 10.00% Injection Carry Over Area carry over 1.00% Height carry over 1.00% Signal to Noise S/N 1,000 S/N 2,000 S/N 400 S/N 100 S/N 1,000 S/N 100 S/N 20 * 1 ul for the 6460A Series w/ AJST; 0.5 ul for the G6490A and G6495A w/ajst 6410, K w/o HotBox 6410, K w/ HotBox A, 6460A AJST, ES, MM G6490 w/ AJST G6495 w/ AJST G6490, G6495 w/ ESI, MM(ES) G6490, G6495 w/ CI, MM(CI), PI G6490A w/ AJST Other QQQs w/ AJST, ES, MM(ES), PI Other QQQs w/ci, MM(CI) Ion Trap: Standard OQ Test Suite Test Setpoints and Parameters Limits Model(s) and/or Source(s) Vacuum Verification N/A High vacuum 8E-6 torr and 2E-5 torr (Pos. and neg. modes) Model-specific; see Appendix: Masses for section Used masses ± 0.2 m/z Response Linearity Injection volume on column: 5 ul* Coeff. of det. (r2) Injection Precision Evaluated mass is most abundant: Area RSD 10.00% 156, 218, or 245 m/z Injection Carry Over Area carry over 1.00% Height carry over 1.00% * For CI source, use 20 ul ES Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 2 of 10

3 TOF: Standard OQ Test Suite Test Setpoints and Parameters Limits Vacuum Verification N/A High vacuum 7E-8 torr and 6E-7 torr (pos. and neg. modes) Model-specific; see Appendix: Masses for Scan Verification section Used masses ± 3.0 ppm Model(s) and/or Source(s) AJST, CI, DSAJST, DSCI, DSES, MM Response Linearity Injection volume on column: Coeff. of det. (r2): AJST, DSAJST, DSES Injection Precision 20 ul* Area RSD 10.00% Injection Carry Over Area carry over 1.00% Evaluated mass is m/z Height carry over 1.00% Signal to Noise S/N 100 S/N 20 * 10 ul for 6230 Series w/ AJST, DSAJST AJST, DSAJST, DSES AJST, DSAJST, DSES, ES, MM(ES) CI, DSCI, MM(CI) Q-TOF: Standard OQ Test Suite Test Vacuum Verification (two zones) (pos. and neg. modes, two zones) Setpoints and Parameters N/A Model-specific; see Appendix: Masses for section Limits High vacuum 8E-6 torr 5.5E-5 torr (quad) High vacuum 8E-6 torr 6.5E-5 torr (quad) High vacuum 8E-6 torr 4E-5 torr (quad) High vacuum 7E-8 torr 5E-7 torr (TOF) Used masses ± 0.2 m/z (quad) Used masses: ± 3.0 ppm (TOF) Response Linearity Injection volume on Coeff. of det. (r2) Injection Precision column: 20 ul* Area RSD: 20.00% Injection Carry Over Area carry over 1.00% Evaluated mass is m/z Height carry over 1.00% Signal to Noise S/N 100 S/N 20 S/N 10 * 10 ul for 6530 Series, 6540 Series w/ AJST, DSAJST; 1 ul for 6550 Series w/ajst, DSAJST Model(s) and/or Source(s) G6550 G6538, G6540, G6545 Other Q-TOFs AJST, DSAJST, DSES AJST, DSAJST, DSES G6550 w/ AJST, DSAJST Other Q-TOFs w/ AJST, DSAJST Other Q-TOFs w/ CI, DSCI, ES, MM Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 3 of 10

4 Test Design and Rationale Many GMP/GLP enforcement agency inspectors now ask firms to provide a risk assessment of their equipment and computer systems plus a science-based rationale for subsequent validation and qualification testing. GENERAL RISK STATEMENT: HPLC, LCMS, UHPLC, UHPLC_MS, GC, or GCMS system used for raw material testing or final drug product / medical device testing in GMP or used in formal GLP studies will likely fall into a HIGH RISK category. This risk assessment will imply the need for IQ & OQ & on-going qualification. ANY USER SPECIFIC RISK ANALYSIS SUPERCEDES THIS GENERAL RISK STATEMENT. The rest of this section outlines the science-based rationale for each test in the Agilent hardware OQ plus a brief test design and procedure description. The recommended set of hardware OQ tests described in this EQP derives from Agilent s interpretation of FDA, USP, and GAMP4 guidelines and other authoritative expert literature. OQ test design incorporates both modular and holistic testing, which is a proven and regulatory acceptable approach. Direct metrology is used to test pump flow rates and thermal-controlled column compartment and autosampler modules. Holistic chemical testing is used for the evaluation of the following critical instrument characteristics: linearity, precision, signal to noise, and carry over. Certified reference standards and calibrated traceable thermometers and digital flow meters are used. Considering the number of setpoints, parameters, and conditions of each recommended OQ test, the proven concepts of worst case, range, and representative have been applied. If a property or characteristic is known to have its worst performance at one end of a range of use, this is the setpoint that should be tested and other setpoints are not required. If a property or characteristic has no known worst case, testing at the high and low points of the range of use is required. If there are too many possible use cases and conditions to realistically test (and none is a worst case), a representative sample for test is the best approach. Vacuum Verification Description: A stable, high vacuum is required for high-sensitivity mass spectrometry. Procedure: Multiple readings of the vacuum system are taken and an automated comparison of these values to the known acceptable values is made. Passing this test is a pre-requisite for the following tests. Description: Calibration of mass range is critical in qualitative mass spectrometry. Procedure: The built-in Agilent autotune is performed to determine the proper calibration of the MS and ensure that masses are correctly reported across the entire mass range of the instrument. Response Linearity Description: Knowledge of the response curve is critical for quantitative analysis. Procedure: A sulfa drug mix standard of four sulfonamide drugs is injected into the system at five concentrations representing a wide range for LCMS. The ions monitored are appropriate to the system type. The calculated RSQ best-fit regression line and plot of the response curve provides the statistics required to evaluate the instrument s overall response curve. This allows users to set appropriate calibration ranges and limits in their quantitative application methods. Injection Precision Description: System precision is critical for accuracy of quantitation. Autosampler performance and MS ionization contribute to LCMS system precision. Autosampler precision is challenged in the standard LC module tests using a UV detector. A repeat precision test in MS mode further challenges the precision of source ionization and MS detection. Procedure: A blank injection followed by six repeat injections of the sulfa drug mix followed by a final blank injection is made. The % RSD of the six injections is calculated to provide precision statistics. Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 4 of 10

5 Carry Over Description: Low carry over from a previous injection is critical for accuracy of quantitative and reliability of qualitative analysis. Autosampler performance and MS condition contribute to LCMS carry over. Autosampler carryover is challenged in the standard LC module tests using a UV detector. A repeat carry over test in MS mode further challenges the full LCMS system carry over performance. Procedure: A blank injection followed by single injection of the highest concentration standard followed by a blank injection. The last blank injection is evaluated for carry over and the result expressed as a percentage of the value for the standard injection. Signal to Noise Description: Sensitivity of MS detection is an important performance feature in quantitative and qualitative analysis. A signalto-noise value of representative compounds and appropriate ions at known concentration provides sensitivity statistics. Procedure: For all newly installed Agilent LCMS systems, a reserpine chemical standard is injected as part of the instrument checkout test to provide a starting sensitivity reading. The reserpine signal-to-noise result is provided separately to the OQ report but can be attached to the OQ report if required. For OQ at installation and ongoing OQ/recalibration, the signal-to-noise value of the sulfa drug mix is reported at the ion of interest. System performance over time can be evaluated by repeating this OQ test at suitable intervals. Updated: February Information, descriptions, and specifications in this publication are subject to change without notice. Agilent Technologies, Inc Published in USA Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 5 of 10

6 Appendix: Masses for Single Quadrupole G6110A/G6120A/G6120B/G6120V/G1946B/G1946C/G1956A with ES source m/z m/z G6110A/G6120A/G6120B/G1946B/G1946C/G1956A with CI, MM, or PI source m/z m/z m/z G6130A/G6130B/G1946D/G1956B with ES source m/z m/z m/z m/z G6130A/G6130B/G1946D/G1956B with CI, MM, or PI source m/z m/z m/z m/z m/z G6140A/G6150B with ES source m/z m/z N/A m/z G6140A/G6150B with MM source m/z m/z N/A m/z G6140A/G6150B with CI or PI source m/z m/z m/z N/A m/z Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 6 of 10

7 Triple Quadrupole: Q1 and Q3 G6410A with ES source m/z G6410A-2K/G6410B/G6420A with ES source m/z G6430A with ES source m/z G6460A/C with ES source m/z m/z G6490A with ES source G6495A with ES source m/z m/z m/z G6410A with MM source m/z Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 7 of 10

8 m/z G6410A-2K/G6410B/G6420A with MM source m/z m/z G6430A with MM source m/z m/z G6460A/C with MM source m/z m/z m/z Ion Trap ES source m/z m/z m/z N/A m/z Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 8 of 10

9 TOF ES, DSAJST, or DSES source ppm ppm ppm ppm ppm ppm ppm ppm ppm ppm CI or DSCI source ppm ppm ppm ppm ppm ppm ppm ppm MM source ppm ppm ppm ppm ppm ppm ppm ppm ppm ppm Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 9 of 10

10 Q-TOF: Quad and TOF Zones DSAJST. DSES. or ES source m/z m/z m/z m/z m/z m/z m/z m/z m/z m/z Agilent Technologies Inc. 2015: no reproduction, translation, or use without permission Page 10 of 10