Ozone in Complementary Medicine Possible Mechanism of Ozone in Rheumatic and Inflammatory Diseases

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1 Ozone in Complementary Medicine Possible Mechanism of Ozone in Rheumatic and Inflammatory Diseases OZONOSAN 1

2 Mechanism of Action in Rheumatic Pain Syndroms Acute stage: Activation of cell metabolism, ATP-increase: rapid subsidence of edema Chronic inflammatory processes: - Immunomodulation, endogenous cytokin therapy on physiological release of eg IFN-ß and TGF-ß, - Activation of biological antioxidants and radical scavengers OZONOSAN 2

3 Immunocompetent cells Monocyten Makrophagen B-Zellen M Activated immunocompetent cells release their specific cytokines e.g. TH 1 -cells: IL-2 and IFN-y TH 2 -cells: IL- 4 and IL-10 as antagonists of IL-2 and IFN-y The activation by ozone thus means an IMMUNMODULATION Waßer OZONOSAN 3

4 Induction of IFN-ß ( in iu/ml at 42µg/ml O 3 ) as antagonist and suppressor of the TH 1 cell function IL-2 IFN-ß Control Ozone BOCCI

5 Induction of TGF-ß in full blood under Ozone application TGF-ß in pg/ml Ozon in µg/ml Bocci

6 Transforming Growth Factor TGF-ß Concentration dependent increase under Ozone released from macrophages and platelets at the site of damage/inflammation, produced from cartilage cells, autoinduction OZONOSAN 6

7 TGF-ß is Responsible for Improved Wound Healing TGF-ß stimulates the production of matrix proteins such as collagen,proteoglycans and hyaluronic acid modulates the ratio protease/proteaseinhibitors activates the surface integrines improves the cell/matrix interaction and the arrangement of matrix molecules has an inherent self-induction through the activation of different cells eg cartilage cells OZONOSAN 7

8 Ozone Activates the Biological Antioxidants and Radical Scavengers and improves the antioxidative capacity of the biological system by increase and activation of superoxidedismutase SOD, catalase, gutathionperoxidase and reductase or glucse-6-phosphate dehydrogenase removing superoxide radicals and OH-radicals, which are responsible as inflammation mediators for the degenerative sequels occurring eg in the cartilage OZONOSAN 8

9 Reactive Oxygen Spezies ROS in the biological System

10 3.1 Upregulation of the biological antioxidans system Pentose- Phospatepathway: production of antioxidative enzymes to protect the cell from oxidative dysstress

11 Enzymatic antioxydants in the biological system and their functions OZONOSAN 11

12 3.1 Upregulation of the biological antioxidans system (Bocci 2004) 250 % GSH-Px GSH-R GSH-Tr SOD

13 Mechanism of Action 3 Enzymatic Antioxydants and Radical Scavengers -such as SOD, GSH-peroxidase and GSH - reductase...- are induced and activated by Ozoneformed peroxides, thus increasing the organism`s antioxydative capacity.

14 OZONE and Prevention (León et al. 2002) Histological results correspond completely to biochemical measurements a b c Rectal Ozone Insufflation in an animal model. Glycogen Depletion in Liver Cells. a) Control b) CCl 4 -induced Glycogen-Depletion c) 15 preventive Ozone Application

15 Protection Against Hepatic Cellular Damage induced by Carbontetrachloride in an Animal Model through 10 rectal Insufflations before CCl 4 Application. (León et al. 1998) SOD (u/g) control CCl 4 O 3 +CCl 4 O 2 +CCl 4

16 Hepatic Ischemia-Reperfusion and Ozone pretreatment H 2 O 2 SOD GSH Control Ischaemia/reperfusion I/R+Ozone Peralta, Leon et al

17 Reperfusion Damage in Renal Cells (Calunga et al.2001) Control Isch/Reperf. O 2 + I/R O 3 + I/R

18 Ozone effect on the antioxidants Vitamin E and Vitamin C 120% Vitaminconcentration in % 100% 80% 60% 40% 20% Vit. E 0% 0h 0,5h 1h 1,5h 2h 2,5h 3h 3,5 4h Contacttime in hours Vit. C Cross et al

19 Mechanism of Action in Rheumatic Pain Syndroms Acute stage: Activation of cell metabolism, ATP-increase: rapid subsidence of edema Chronic inflammatory processes: - Immunomodulation, endogenous cytokin therapy on physiological release of eg IFN-ß and TGF-ß, - Activation of biological antioxidants and radical scavengers OZONOSAN 19

20 Thank you for your attention OZONOSAN 20