C146-E121A LCMS Shimadzu Liquid Chromatograph Mass Spectrometer

Transcription

1 146-E121 LMS-2020 Shimadzu Liquid hromatograph Mass Spectrometer

2 Seeing is elieving. LMS-2020

3 LMS-2020 Shimadzu Liquid hromatograph Mass Spectrometer Seeing is elieving. uilding an L/MS system that employs an MS as an L detector can effectively reduce the limitations of L analysis. In addition to this, reducing those limitations to the greatest extent possible requires an MS that can handle ultra high speed analysis. The new "LMS-2020" single quadrupole ultra-fast L/MS, boldly addresses these requirements. It is capable of providing more data points and faster scans than any previous quadrupole LMS instrument, allowing it to handle the fastest small-particle and high efficiency columns. ontents P 4 - UFL Quality: Three Points for Ultra-fast, High-sensitivity MS Measurements P 8 - Ultra-fast UFL / MS Measurements using UFswitching / UFscanning P 9 - Hardware Features that to Powerfully Support Three UFs P10- Software LabSolutions LMS Ver.5 P12- Open Solution P13- Diverse Ionization Methods Expand the Range of pplications

4 Features UFL Quality From HPL to UFL. Then to UFL/MS. From HPL to UFL UFL achieves excellent speed and resolution, while offering the high precision not available with conventional HPL and expandability options. Ultra Fast Not only high-speed analysis, but increased overall speed through rapid sample injection and fully automatic analysis functions. Unquestionable Fidelity UFL offers exceptional injection reproducibility as well as ultra high-speed operation. In terms of minimizing sample carryover, essential in L/MS analysis, the LMS-2020 stays ahead of the competition. Ultra Flexible overs an extensive range from ultra-fast analysis to conventional HPL and semi-preparative analysis. 4

5 Ultra Fast - UFscanning, UFswitching, and 5

6 Speed is Power Greater speed. Greater sensitivity. Ionization Probe Desolvation Line Skimmer Entrance Lens Pre-rod Octopole Ion Source Qarray Ultra-fast UFswitching Rapid 15-millisecond positive/negative ionization switching Positive-ion measurement SIM 2H Polarity switching time 15 msec Negative-ion measurement SIM 2H Polarity switching time 15 msec Positive-ion measurement SIM 2H To detect both positive and negative ions, analysis is performed while switching between the positive and negative ionization modes. The LMS-2020 adopts a high-voltage power supply featuring novel technology (patent pending) to achieve an ultra-fast polarity switching time of just 15 ms. Sample: 6

7 ccurate mass analysis of sharp chromatographic peaks obtained by UFL requires ultra-fast MS detection capabilities. The LMS-2020 offers UFswitching for rapid switching between the positive and negative ionization modes and UFscanning for ultra-fast scan measurements to capture the sharpest UFL peaks. Main-rod Detector Enhances sensitivity by preventing ion divergence Quadrupole Ultra-fast UFsensitivity Superior sensitivity from UFL The newly developed Qarray Optics achieves superior sensitivity, reproducibility, and linearity. Ultra-fast UFscanning 15,000 u/sec fast scanning speed ontrols the voltage applied to the Quadrupole according to the scan speed and m/z. This new technology (patent pending) maintains resolution and achieves high ion transmittance even at high scanning speeds. alibration curve 7

8 UFsensitivity UFscanning & UFswitching Necessity of UFswitching and UFscanning for ultra-fast analysis Ultra-fast detection (MS measurement) is required for ultra-fast analysis with elution of six components per minute, for example. The UFswitching and UFscanning functions permit the required ultra-fast mass spectrometry. Polarity switching time 15 msec Polarity switching time 15 msec Positive-ion measurement 15,000 u/sec Negative-ion measurement 15,000 u/sec Positive-ion measurement 15,000 u/sec Examples of ionization in positive and negative modes MS Spectra of entazone MS Spectra of Dymuron MS Spectra of arpropamid 8

9 Hardware Features that to Powerfully Support Three UFs Toughness against dirty samples In order to check the toughness of the LMS-2020 against dirty samples, plasma samples simply precipitated with only acetonitrile were injected 2,500 times over 10 days (1μL volume per injection). Excellent reproducibility of peak area was demonstrated and its RSD was 2.26%. Easy Maintenance The desolvation line (DL) that introduces the sample from the ion source into the vacuum can be installed and uninstalled without breaking the vacuum, which dramatically enhances ease-of-maintenance. DL Vacuum region %RSD2.26 Internal standard nalysis time 6 min 2500 injections over 10 days Retaining the vacuum even if the DL is uninstalled reating Fragment Ions by In-source ID In-source ID (collision-induced dissociation) is effective for confirming the molecular weight of synthetic compounds and for the quantification of impurities. MS hromatogram for Erythromycin Measurements DL=0V, Qarray D=0V Erythromycin (major component) Magnified view Erythromycin (major component) Using in-source ID allows the generation of fragment ions. This example shows the composition of impurities in erythromycin estimated from fragment ions generated by in-source ID. The multi-sequence mode permits several other methods within a single analysis, such as ID, positive/negative ion switching modes, and SN/SIM modes. Precisely setting the parameters reduces the risk of erroneous evaluations and enhances the reliability of analysis results. MS Spectra (Normal mode) DL=0V, Qarray D=0V MS Spectra (In-source ID mode) DL=0V, Qarray D=60V Erythromycin (major component) Peak1 85-(8)-Hydroxy-Erythromycin 9

10 Software LabSolutions LMS Ver.5 Powerful support for UFL/LMS-2020 high-speed performance. This software maximizes analysis performance. Rapidly analyzes huge volumes of data in browser windows. The comprehensive, clear display provides a stress-free working environment. omparison of ontrol and Target Multiple data items are displayed sequentially on the same screen. To view the diverse information in a data file in the optimal layout for comparison, the data can be browsed like flipping through the pages of a book to discover differences between the data. ontrol Target Peak table Peak Integration Manual peak integration can be conducted on both L and MS chromatograms simultaneously. oth the peak table and MS spectrum table are displayed. The peak table and chromatograms/spectra are interlinked for easier operation. MS spectrum table Manual peak integration bar 10

11 Optimization of nalysis Parameters utomatically searches and sets the voltages that affect the ion transmittance (DL/Qarray voltage) to the optimal values for the target compounds. SIM Table Enter the target m/z Method Optimization Window Specify the conditions to search for the optimal voltage utomatic analysis execution Method Optimization Result Window The Search results of the optimal voltage value are displayed SIM Table The optimal voltage value is set into the SIM table automatically nalytical conditions are optimized 11

12 Open Solution reate an Open ccess Environment Using Internet Explorer heck nalysis Results Using Internet Explorer Running the optional Open Solution software on a P with LabSolutions LMS installed makes it possible to check analysis results and print reports from any networked P. Special software installation on each P and additional license contracts are not required. LN UFL Laboratory LMS LMS-2020 Office hromatogram and spectra can be magnified and contracted. Printing and PDF file creation are possible. lick on a peak in the mass chromatogram to display the spectrum at that point. hromatogram (MS) Mass spectrum Sample rack lick on a peak in the PD data to display the spectrum at that point. hromatogram (L) UV spectrum lick on a target peak in the peak table. The corresponding peak in the chromatogram is highlighted in color, and the spectrum for the peak is also displayed. Peak table Target MS table When the target compound is detected, it is flagged with a green chekmark in the target MS table. lick on a target vial to display the result (chromatogram, spectrum, and peak table). 12

13 Ionization Methods Diverse Ionization Methods Expand the Range of pplications Selecting the ionization method appropriate for the target compound achieves superior analysis results. LMS-2020 offers PI and DUIS in addition to ESI. Diverse ionization methods support a wide range of applications. Selecting the most appropriate Ionization Method ompounds Properties DN Proteins Peptides mino acids Macromolecules arbohydrates Triglycerides romatic hydrocarbons liphatic hydrocarbons Polar Non-polar Volatile Non-volatile Thermostable Thermolabile ESI ESI PI PI DUIS DUIS Functional groups cid lcohol ldehyde lkane lkyne mino arbonyl Ester Ether Phenol ESI PI DUIS Extremely suitable Suitable nalysis possible with appropriate parameters Inherently unsuitable Ionization Options PI-2020 Sample injection While the water-soluble vitamins thiamine and riboflavin can be detected by ESI, they are virtually undetectable by PI. onversely, the fat-soluble vitamin calciferol can be detected by PI but ESI does not offer adequate detection sensitivity. DUIS-2020 allows detection of a mixture of compounds suited to ESI or PI, without missing any compounds. MS hromatograms orona needle DUIS-2020 ESI probe MS Spectra from DUIS Measurements Thiamine Riboflavin alciferol orona needle Mixed Sample of Three Water-soluble/Fat-soluble Vitamins 1. Thiamine: m/z 265; cation, generated by ionization, water-soluble vitamin 2. Riboflavin: m/z 377; protonated molecule, water-soluble vitamin 3. alciferol: m/z 397; protonated molecule, fat-soluble vitamin 13

14 JQ-0376 Founded in 1875, Shimadzu orporation, a leader in the development of advanced technologies, has a distinguished history of innovation built on the foundation of contributing to society through science and technology. We maintain a global network of sales, service, technical support and applications centers on six continents, and have established long-term relationships with a host of highly trained distributors located in over 100 countries. For information about Shimadzu, and to contact your local office, please visit our Web site at SHIMDZU ORPORTION. International Marketing Division 3. Kanda-Nishikicho 1-chome, hiyoda-ku, Tokyo , Japan Phone: 81(3) Fax. 81(3) URL The contents of this brochure are subject to change without notice. Printed in Japan NS